                         SEQUENCE LISTING

<110>  Cambrex IEP GmbH
 
<120>  Biocatalytic process for the production of (R)-3-Quinuclidinol

<130>  MB

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  256
<212>  PRT
<213>  Mycobacterium vanbaalenii

<400>  1

Met Thr Arg Thr Val Val Val Thr Gly Ala Gly Ser Gly Ile Gly Arg 
1               5                   10                  15      


Ala Ile Ala Asn Thr Leu Ala Gln Arg Asn Trp Arg Val Val Val Thr 
            20                  25                  30          


Asp Val Asn Ala Ala Ala Ala Ser Glu Val Ala Ala Ala Leu Pro Asn 
        35                  40                  45              


Pro Pro Ala Gly His Glu Ser Ala Gln Leu Asp Val Thr Ser Pro Glu 
    50                  55                  60                  


Asn Ala Ala Ala Val Ala Ser Asp Val Ser Glu Arg Leu Gly Leu Asp 
65                  70                  75                  80  


Ala Trp Val Ser Asn Ala Gly Ile Ser Phe Met Arg Arg Phe Leu Asp 
                85                  90                  95      


Ala Pro Ile Glu Arg Tyr Glu Gln Thr Met Asp Val Asn Leu Lys Gly 
            100                 105                 110         


Val Phe Val Cys Gly Gln Ala Ala Ala Arg Glu Met Val Arg Ala Gly 
        115                 120                 125             


Ile Pro Gly Met Ile Val Asn Thr Ala Ser Met Ala Gly Lys Gln Gly 
    130                 135                 140                 


Arg Val Pro Phe Leu Ser Asp Tyr Val Ala Ser Lys Phe Gly Val Val 
145                 150                 155                 160 


Gly Leu Thr Gln Ala Met Ala Tyr Glu Leu Gly Glu His Gly Ile Thr 
                165                 170                 175     


Val Asn Cys Val Cys Pro Gly Phe Val Glu Thr Pro Met Gln Ser Arg 
            180                 185                 190         


Glu Leu Gln Trp Glu Ala Glu Leu Arg Gly Thr Thr Pro Asp Gly Val 
        195                 200                 205             


Arg Ala Met Met Ile Ala Asp Thr Pro Leu Gly Arg Leu Glu Gln Ala 
    210                 215                 220                 


Asp Asp Val Ala Arg Ala Val Ala Phe Leu Leu Ser Asp Asp Ala Arg 
225                 230                 235                 240 


Phe Ile Thr Gly Glu Ala Leu Ala Val Asn Gly Gly Ala Tyr Met Asp 
                245                 250                 255     


<210>  2
<211>  771
<212>  DNA
<213>  Mycobacterium vanbaalenii

<400>  2
atgacgagaa ctgtggtcgt gaccggagcg ggatccggta tcggacgagc gatcgccaac       60

acactggcgc aacgaaattg gcgggtggtg gtcaccgacg tcaatgccgc cgccgcaagc      120

gaggtcgcag cggcactgcc gaatccgccc gcaggccatg aatccgcaca actggacgtc      180

acctcccccg agaacgcggc cgccgtggca tccgacgtct cggagcggct cgggctcgac      240

gcgtgggtga gcaacgccgg catctcgttc atgcgccgct tcctcgacgc ccccatcgag      300

cgctacgagc agaccatgga cgtgaatctc aagggcgtgt tcgtgtgcgg gcaggccgcg      360

gcgcgggaga tggtgcgcgc cggcattccc gggatgatcg tgaacaccgc ctcgatggcg      420

ggcaagcagg gccgggtgcc gttcctgtcg gactacgtcg cctcgaaatt cggggtggtc      480

gggttgaccc aggcgatggc ctacgaactc ggcgagcatg gcatcacagt caactgcgtg      540

tgccctggct ttgtcgaaac ccccatgcag tcaagggaat tgcagtggga ggccgagctg      600

cgtggcacca cccccgacgg tgtccgcgcc atgatgatcg ccgacacgcc gctgggtcgc      660

cttgagcagg ccgacgacgt cgcccgcgcc gtggccttcc tgctctccga cgacgcccgt      720

ttcatcaccg gcgaagcgct cgccgtcaac ggcggcgcct acatggactg a               771


<210>  3
<211>  264
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  3

Met Ser Thr Pro Ala Asn Ser Thr Pro Asp Thr Pro Ser Thr Val Val 
1               5                   10                  15      


Val Thr Gly Ala Gly Ser Gly Ile Gly Arg Ala Ile Ala Thr Thr Leu 
            20                  25                  30          


Ala Glu Arg Gly Trp Arg Val Val Ala Thr Asp Val Asp Val Ala Ala 
        35                  40                  45              


Ala Asp Glu Thr Ala Ser Ser Leu Ser Gly Ser Gly His Glu Ser Ala 
    50                  55                  60                  


Arg Leu Asp Val Thr Asp Pro Ala Gln Ala Ser Ala Leu Ala Asp Asp 
65                  70                  75                  80  


Val Ala Asp Arg Ile Gly Leu Gly Ala Trp Val Ser Asn Ala Gly Ile 
                85                  90                  95      


Ser Ala Met Ala His Phe Val Asp Ile Ser Ala Glu Gln Leu Asp Arg 
            100                 105                 110         


Ser Leu Asp Val Asn Leu Lys Gly Val Phe Phe Cys Gly Gln Ala Ala 
        115                 120                 125             


Ala Arg Ala Met Ile Arg Thr Gly Val Arg Gly Thr Ile Val Asn Thr 
    130                 135                 140                 


Ala Ser Met Ala Ala Lys Gln Gly Arg Val Pro Phe Leu Ala Asp Tyr 
145                 150                 155                 160 


Val Ala Ser Lys Phe Gly Val Leu Gly Leu Thr Gln Ala Met Ala Phe 
                165                 170                 175     


Glu Leu Ala Ala His Gly Ile Thr Val Asn Ser Val Cys Pro Gly Phe 
            180                 185                 190         


Val Ala Thr Pro Met Gln Thr Arg Glu Leu Glu Trp Glu Ala Lys Leu 
        195                 200                 205             


Ser Gly Ser Thr Pro Asp Gly Val Arg Gln Ser Trp Ile Asp Ala Thr 
    210                 215                 220                 


Pro Leu Gly Arg Leu Gln Thr Pro Asp Asp Val Ala Arg Ala Val Ala 
225                 230                 235                 240 


Phe Leu Val Ser Glu Asp Ala Arg Phe Ile Thr Gly Glu Ala Leu Ser 
                245                 250                 255     


Val Asn Gly Gly Ala Tyr Met Asp 
            260                 


<210>  4
<211>  795
<212>  DNA
<213>  Mycobacterium smegmatis

<400>  4
atgagcaccc ccgcgaacag cactcccgac acccccagca ccgtcgtcgt caccggcgcc       60

ggatccggca tcggccgcgc gatcgccacc acgctcgccg aacgcggctg gcgtgtggtc      120

gccaccgacg tcgacgtcgc cgcggccgac gagacggcct catccctgag cggcagcggc      180

cacgagtcgg cgcgtctcga cgtcaccgat cccgcgcagg cgtccgcact ggccgacgac      240

gtcgccgacc gcatcgggct gggggcctgg gtcagcaacg cgggcatctc cgcgatggcg      300

cacttcgtcg acatctccgc cgaacaactc gaccgcagtc tcgacgtcaa cctcaagggc      360

gtgttcttct gtggtcaggc cgcggcccgc gccatgatcc gcaccggtgt gcgcggcacg      420

atcgtcaaca ccgcatcgat ggcggcaaag cagggccggg taccgttcct cgcggactat      480

gtcgcgtcca agttcggcgt gctgggactc acccaggcga tggccttcga acttgccgca      540

cacggcatca cggtcaacag tgtctgcccc ggtttcgtgg caacaccgat gcagacacga      600

gaattggagt gggaggcgaa actcagcggc tcgacgcccg acggcgtacg ccagtcctgg      660

atcgacgcga cgccgctggg ccgcctgcaa actcccgacg acgtcgcccg cgcggtggcg      720

ttcctggtct ccgaagatgc ccgcttcatc accggagagg cgctgtcggt caacggcggt      780

gcctacatgg actga                                                       795


<210>  5
<211>  259
<212>  PRT
<213>  Caldilinea aerophila

<400>  5

Met Arg Leu Arg Gly Lys Ile Ile Gly Ile Thr Gly Ala Gly Ser Gly 
1               5                   10                  15      


Ile Gly Lys Ala Ala Ala Leu Ala Leu Ala Arg Glu Gly Ala Ala Leu 
            20                  25                  30          


Ala Val Thr Asp Phe Asn Leu Asp Trp Ala Gln Gln Thr Ala Ala Asp 
        35                  40                  45              


Ile Arg Ser Gln Gly Gly Glu Ala Thr Ala Trp Arg Leu Asp Val Thr 
    50                  55                  60                  


Asp Gln Ala Glu Ala Glu Arg Val Leu Gln Ala Leu Ile Asp Arg Tyr 
65                  70                  75                  80  


Gly Arg Leu Asp Val Trp Val Asn Asn Ala Gly Val Ser Thr Met Asn 
                85                  90                  95      


Arg Phe Val Asp Leu Thr Glu Arg Asp Trp Asp Tyr Asn Met Asn Val 
            100                 105                 110         


Asn Ala Lys Gly Val Phe Leu Cys Ser Gln Val Ala Ala Arg Arg Met 
        115                 120                 125             


Ile Ala Gln Gly Gly Gly Lys Ile Ile Asn Val Ala Ser Met Ala Gly 
    130                 135                 140                 


Lys Arg Gly Asn Ala Pro Phe Leu Ala His Tyr Val Ala Ser Lys Phe 
145                 150                 155                 160 


Ala Val Ile Gly Leu Thr Gln Ala Met Ala Gly Glu Leu Ala Pro Tyr 
                165                 170                 175     


His Ile Thr Val Asn Ala Val Cys Pro Gly Tyr Val Arg Thr Ser Met 
            180                 185                 190         


Gln Glu Arg Glu Val Glu Trp Glu Ala Met Leu Arg Gly Val Thr Pro 
        195                 200                 205             


Glu Ala Val Arg Gln Leu Tyr Ile Gln Asp Thr Pro Leu Arg Arg Leu 
    210                 215                 220                 


Glu Thr Pro Glu Asp Val Ala Lys Val Ile Val Phe Leu Ala Ser Glu 
225                 230                 235                 240 


Asp Ala Asp Phe Ile Thr Gly Glu Ala Ile Asn Val Asn Gly Gly Ala 
                245                 250                 255     


Trp Met Asp 
            


<210>  6
<211>  780
<212>  DNA
<213>  Caldilinea aerophila

<400>  6
atgcgactca ggggcaaaat tatcggcatc accggcgctg gttcaggcat tggcaaggcg       60

gcggctctag cgctggcgcg ggagggcgcc gcattggccg tgacggactt caacctggat      120

tgggcgcagc aaacagccgc agacatccgc agccagggcg gcgaggcgac agcatggcgg      180

cttgacgtga ccgaccaggc cgaggcagag cgcgtgctgc aggcgctgat cgaccgttac      240

ggccggctcg acgtctgggt gaacaacgca ggcgtctcga ccatgaaccg ctttgttgac      300

ctcactgagc gggactggga ctataacatg aacgtcaacg ccaaaggcgt ttttctctgc      360

agccaggtcg ccgcccgacg catgatcgcc caaggaggtg ggaagatcat caacgtcgcc      420

tccatggcag gcaaacgagg caacgcacct ttcctggctc attatgttgc cagcaaattt      480

gctgtaatcg ggctgacgca agcgatggcg ggcgaactgg cgccttatca catcaccgtc      540

aacgccgttt gccctggcta tgtgcgcaca tctatgcaag agcgagaggt ggagtgggag      600

gcaatgctgc gtggggttac acccgaagcg gtgcgacaac tgtatatcca ggatacacct      660

ctgcgacgcc tcgaaacacc cgaagatgtg gcgaaagtca ttgtttttct tgcttcagag      720

gacgcagact tcatcacggg cgaagcgatt aacgtcaacg gcggcgcgtg gatggattaa      780


<210>  7
<211>  266
<212>  PRT
<213>  Starkea novella

<400>  7

Met Ile Pro Ala Thr Ala Pro Thr Pro Val Tyr Pro Glu Leu Ser Gly 
1               5                   10                  15      


Arg Leu Ala Phe Val Thr Gly Ala Ala Thr Gly Ile Gly Arg Ala Ile 
            20                  25                  30          


Ala Thr Ala Leu Ala Arg Gln Gly Val Arg Val Ala Ile Gly Asp Ile 
        35                  40                  45              


Asn Leu Gly Ala Ala Glu Asp Ala Ala Ala Ala Ile Gly Gly Gly Ala 
    50                  55                  60                  


Val Ala Val Glu Val Asp Val Arg Arg Arg Ala Ser Val Glu Ala Ala 
65                  70                  75                  80  


Phe Ala Arg Val Leu Glu Leu Leu Gly Gly Cys Asp Leu Leu Val Ala 
                85                  90                  95      


Asn Ala Gly Val Ser Thr Met Gln Ala Ala Leu Glu Ile Thr Asp Gln 
            100                 105                 110         


Glu Trp Asp Phe Asn Phe Asp Val Asn Thr Arg Gly Val Phe Leu Thr 
        115                 120                 125             


Asn Gln Ile Val Ala Arg His Phe Val Ala Thr Gly Lys Gly Cys Ile 
    130                 135                 140                 


Val Asn Thr Ala Ser Leu Ala Ala Lys Val Gly Ala Pro Leu Leu Ala 
145                 150                 155                 160 


His Tyr Ser Ala Ser Lys Phe Ala Val Leu Gly Trp Thr Gln Ala Leu 
                165                 170                 175     


Ala Arg Glu Leu Ala Pro Lys Gly Ile Arg Val Asn Ala Val Cys Pro 
            180                 185                 190         


Gly Phe Val Ala Thr Gly Met Gln Ser Arg Glu Val Gln Trp Glu Ala 
        195                 200                 205             


Thr Leu Arg Gly Val Thr Pro Gln Arg Val Ile Asp Asp Tyr Ile Ala 
    210                 215                 220                 


Gln Thr Pro Leu Gly Arg Leu Glu Gln Pro Glu Asp Val Ala Asp Val 
225                 230                 235                 240 


Val Val Phe Leu Cys Ser Glu Gln Ala Arg Phe Met Thr Gly Gln Gly 
                245                 250                 255     


Val Asn Val Thr Gly Gly Val Tyr Thr Thr 
            260                 265     


<210>  8
<211>  801
<212>  DNA
<213>  Starkea novella

<400>  8
atgatccccg cgaccgcccc gacgcccgtc taccccgaac tttccggccg gctggccttc       60

gtgacggggg cggcgacggg catcgggcgt gccatcgcca cggctctggc gcgccagggc      120

gtgcgggtcg cgatcggcga catcaatctc ggcgcggccg aggacgcggc ggcggcgatc      180

ggcggcggtg ccgtggcggt cgaggtggat gtgcgccggc gcgcctccgt cgaggcggcg      240

ttcgcccgcg tgctggagtt gctcggcggc tgcgacctgc tggtcgccaa tgccggcgtc      300

tcgaccatgc aggcggcgct cgagatcacc gaccaggagt gggacttcaa cttcgacgtc      360

aacacccgcg gcgtgttcct caccaaccag atcgtcgccc ggcatttcgt ggcgaccggc      420

aagggctgca tcgtcaacac cgcctcgctc gccgccaagg tcggggcgcc gctgctggcg      480

cattactcgg cctccaaatt cgccgtcctc ggctggacgc aggcgctggc ccgcgagctc      540

gcccccaagg gcatccgcgt caatgccgtc tgcccgggct tcgtggcgac cggcatgcag      600

tcgcgcgagg tgcagtggga ggcgacgctg cgcggcgtga ccccgcagcg cgtgatcgac      660

gactacatcg cccagacccc gctcggccgg ctggagcagc cggaggacgt cgccgacgtg      720

gtggtgttcc tgtgctcgga gcaggcccgc ttcatgaccg ggcagggcgt caacgtcacc      780

ggcggcgtct acacgacctg a                                                801


<210>  9
<211>  264
<212>  PRT
<213>  Oceanithermus profundus

<400>  9

Met Leu Glu Lys Arg Thr Ala Leu Ile Thr Gly Ala Gly Gly Gly Ile 
1               5                   10                  15      


Gly Ala Ala Val Ala His Arg Leu Ala Arg Glu Gly Ala Lys Val Trp 
            20                  25                  30          


Val Thr Asp Arg Asp Leu Asp Ala Ala Glu Ala Thr Ala Ala Ala Ile 
        35                  40                  45              


Arg Glu Ala Gly Gly Arg Ala Arg Ala Arg Arg Val Asp Val Thr Arg 
    50                  55                  60                  


Arg Glu Glu Leu Glu Ala Ala Cys Ala Ala Ala Tyr Ala Glu Asp Gly 
65                  70                  75                  80  


Arg Val Asp Leu Val Val Ala Asn Ala Gly Val Ser Thr Met Arg Pro 
                85                  90                  95      


Phe Leu Glu Leu Thr Asp Glu Asp Trp Ala Phe Asn Phe Asp Val Asn 
            100                 105                 110         


Ala Arg Gly Thr Phe Tyr Thr Leu Gln Thr Phe Ala Arg Arg Met Lys 
        115                 120                 125             


Asp Gln Ala Pro Met Pro Gly Ser Ser Leu Arg Gly Lys Leu Ile Ala 
    130                 135                 140                 


Val Ala Ser Met Ala Ala Arg Gln Ala Ala Pro Trp Leu Ala His Tyr 
145                 150                 155                 160 


Ser Ala Ser Lys Phe Ala Val Leu Gly Leu Val Gln Ala Ala Ala Lys 
                165                 170                 175     


Glu Leu Ala Pro Phe Arg Ile Thr Val Asn Ala Val Asn Pro Gly Phe 
            180                 185                 190         


Val Lys Thr Ser Met Gln Glu Arg Glu Ile Ala Trp Glu Ala Arg Leu 
        195                 200                 205             


Arg Gly Thr Thr Pro Glu Ala Val Val Ala Asp Tyr Leu Ala Gln Thr 
    210                 215                 220                 


Pro Leu Gly Arg Leu Glu Arg Pro Glu Asp Val Ala Gly Val Val Ala 
225                 230                 235                 240 


Phe Leu Ala Gly Pro Asp Ala Asp Phe Ile Thr Gly Glu Ala Val Glu 
                245                 250                 255     


Val Asn Gly Gly Ala Trp Ile Phe 
            260                 


<210>  10
<211>  795
<212>  DNA
<213>  Oceanithermus profundus

<400>  10
atgcttgaga aaagaacggc actgatcacc ggcgccggcg gcggcatcgg cgccgccgtg       60

gcccaccggc tcgcgcgcga aggcgccaag gtctgggtca cggaccgcga cctcgacgcc      120

gcggaggcga cggccgcggc catccgggag gcgggcgggc gcgcccgcgc gcggcgcgtg      180

gacgtcaccc gccgggaaga gctcgaggcc gcctgcgcag cggcctacgc cgaggacggg      240

cgcgtcgacc tggtggtggc gaacgccggc gtgagcacga tgcgcccctt cctggagctg      300

accgacgagg actgggcgtt caacttcgac gtcaacgccc gcgggacctt ctacaccctg      360

cagaccttcg cccggcgcat gaaggaccag gcgccgatgc cgggaagctc gctgcggggg      420

aagctgatcg ccgtggccag catggcggcg cgccaggcgg ccccctggct ggcccactac      480

tccgcctcga agttcgcggt gctggggctg gtgcaggcgg cggcgaagga gctcgccccc      540

ttccggatca ccgtcaacgc ggtgaacccg ggcttcgtca agacctcgat gcaggaacgc      600

gaaatcgcct gggaggcgcg gctgcggggc accacccccg aggcggtcgt cgccgactac      660

ctggcccaga ccccgctggg acggctggaa aggccggagg acgtcgccgg cgtggtggct      720

ttcctggccg gccccgacgc cgacttcatc accggcgagg ccgtggaggt gaacggcggg      780

gcctggatct tctga                                                       795


