                                SEQUENCE LISTING

<110> Stafford, Ryan
      Thanos, Christopher D.
      Sutro Biopharma, Inc.

<120> The Use of Lambda-Gam Protein in
  Ribosomal Display Technology
  

<130> 91200-906425

<140> WO Not yet assigned
<141> Not yet assigned  

<150> US 61/815,951       
<151> 2013-04-25  

<160> 52

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 98
<212> PRT
<213> Escherichia coli

<220> 
<223> E. coli strain K-12 substrain BW2952 host-nuclease
      inhibitor protein gam, GAM, lambda GamS

<400> 1
Met Asn Ala Tyr Tyr Ile Gln Asp Arg Leu Glu Ala Gln Ser Trp Ala
 1               5                  10                  15      
Arg His Tyr Gln Gln Leu Ala Arg Glu Glu Lys Glu Ala Glu Leu Ala
            20                  25                  30          
Asp Asp Met Glu Lys Gly Ile Pro Gln His Leu Phe Glu Ser Leu Cys
        35                  40                  45              
Ile Asp His Leu Gln Arg His Gly Ala Ser Lys Lys Ser Ile Thr Arg
    50                  55                  60                  
Ala Phe Asp Asp Asp Val Glu Phe Gln Glu Arg Met Ala Glu His Ile
65                  70                  75                  80  
Arg Tyr Met Val Glu Thr Ile Ala His His Gln Val Asp Ile Asp Ser
                85                  90                  95      
Glu Val
        


<210> 2
<211> 324
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic E. coli strain K-12 substrain BW2952
      host-nuclease inhibitor protein gam, GAM, lambda
      GamS with His tag, GamSHis gene

<400> 2
atgaacgctt attacattca ggatcgtctt gaggctcaga gctgggcgcg tcactaccag 60
cagctcgccc gtgaagagaa agaggcagaa ctggcagacg acatggaaaa agggatcccc 120
cagcacctgt ttgaatcgct atgcatcgat catttgcaac gccacggggc cagcaaaaaa 180
tccattaccc gtgcgtttga tgacgatgtt gagtttcagg agcgcatggc agaacacatc 240
cggtacatgg ttgaaaccat tgctcaccac caggttgata ttgattcaga ggtagggggt 300
tctcatcatc atcatcatca ttaa                                        324

<210> 3
<211> 107
<212> PRT
<213> Artificial Sequence

<220> 
<223> synthetic E. coli strain K-12 substrain BW2952
      host-nuclease inhibitor protein gam, GAM, lambda
      GamS with His tag, GamSHis protein

<400> 3
Met Asn Ala Tyr Tyr Ile Gln Asp Arg Leu Glu Ala Gln Ser Trp Ala
 1               5                  10                  15      
Arg His Tyr Gln Gln Leu Ala Arg Glu Glu Lys Glu Ala Glu Leu Ala
            20                  25                  30          
Asp Asp Met Glu Lys Gly Ile Pro Gln His Leu Phe Glu Ser Leu Cys
        35                  40                  45              
Ile Asp His Leu Gln Arg His Gly Ala Ser Lys Lys Ser Ile Thr Arg
    50                  55                  60                  
Ala Phe Asp Asp Asp Val Glu Phe Gln Glu Arg Met Ala Glu His Ile
65                  70                  75                  80  
Arg Tyr Met Val Glu Thr Ile Ala His His Gln Val Asp Ile Asp Ser
                85                  90                  95      
Glu Val Gly Gly Ser His His His His His His
            100                 105         


<210> 4
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H1a For for Fab HC library construction

<400> 4
cgcggcaagc ggttttaaca ttavtrrtwm ykmtatccac tgggtgcgtc aagcac     56

<210> 5
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H1a Rev for Fab HC library construction

<400> 5
gtgcttgacg cacccagtgg atakmrkway yabtaatgtt aaaaccgctt gccgcg     56

<210> 6
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H1bFor for Fab HC library construction

<400> 6
cgcggcaagc ggttttaaca ttavtrrtwm ykggatccac tgggtgcgtc aagcac     56

<210> 7
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H1b Rev for Fab HC library construction

<400> 7
gtgcttgacg cacccagtgg atccmrkway yabtaatgtt aaaaccgctt gccgcg     56

<210> 8
<211> 77
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H2a For for Fab HC library construction

<400> 8
gggtaagggc ctggaatggg ttgstdggat tdmtccgdmt rrcggtdmta ccdactatgc 60
ggatagcgtg aaaggcc                                                77

<210> 9
<211> 77
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H2a Rev for Fab HC library construction

<400> 9
ggcctttcac gctatccgca tagthggtak haccgyyakh cggakhaatc chascaaccc 60
attccaggcc cttaccc                                                77

<210> 10
<211> 77
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H2b For for Fab HC library construction

<400> 10
gggtaagggc ctggaatggg ttgstdhtat tdmtccgdmt rrcggtdmta ccdactatgc 60
ggatagcgtg aaaggcc                                                77

<210> 11
<211> 77
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H2b Rev for Fab HC library construction

<400> 11
ggcctttcac gctatccgca tagthggtak haccgyyakh cggakhaata dhascaaccc 60
attccaggcc cttaccc                                                77

<210> 12
<211> 77
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H2c For for Fab HC library construction

<400> 12
gggtaagggc ctggaatggg ttgstgaaat tdmtccgdmt rrcggtdmta ccdactatgc 60
ggatagcgtg aaaggcc                                                77

<210> 13
<211> 77
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H2c Rev for Fab HC library construction

<400> 13
ggcctttcac gctatccgca tagthggtak haccgyyakh cggakhaatt tcascaaccc 60
attccaggcc cttaccc                                                77

<210> 14
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H3a For for Fab HC library construction

<220> 
<221> modified_base  
<222> (1)...(80)
<223> n = g, a, c or t

<400> 14
gcagaggaca ctgccgtcta ctattgtgcg cgcnnknnkn nknnknnknn knnknnkatg 60
gactactggg gccagggtac                                             80

<210> 15
<211> 80
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H3a Rev for Fab HC library construction

<220> 
<221> modified_base  
<222> (1)...(80)
<223> n = g, a, c or t

<400> 15
gtaccctggc cccagtagtc catmnnmnnm nnmnnmnnmn nmnnmnngcg cgcacaatag 60
tagacggcag tgtcctctgc                                             80

<210> 16
<211> 82
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H3b For for Fab HC library construction

<220> 
<221> modified_base  
<222> (1)...(82)
<223> n = g, a, c or t

<400> 16
gcagaggaca ctgccgtcta ctattgtgcg cgcnnknnkn nknnknnknn knnknnkttt 60
gactactggg gccagggtac gc                                          82

<210> 17
<211> 82
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H3b Rev for Fab HC library construction

<220> 
<221> modified_base  
<222> (1)...(82)
<223> n = g, a, c or t

<400> 17
gcgtaccctg gccccagtag tcaaamnnmn nmnnmnnmnn mnnmnnmnng cgcgcacaat 60
agtagacggc agtgtcctct gc                                          82

<210> 18
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer HER2_FAB_HC_STOP! RP for PCR and RT-PCR

<400> 18
ttattaacaa caagatttcg gctccacct                                   29

<210> 19
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer HER2_FAB_LC_STOP! RP for PCR and RT-PCR

<400> 19
ttattagcac tcaccgcggt taaa                                        24

<210> 20
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer FAB FP for PCR and RT-PCR

<400> 20
atggaagttc aattagtaga aagcggcg                                    28

<210> 21
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer FAB RP for PCR and RT-PCR

<400> 21
acaagatttc ggctccacct tcttg                                       25

<210> 22
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer Fc FP for PCR and RT-PCR

<400> 22
caagaaggtg gagccgaaat cttgt                                       25

<210> 23
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer Fc RP for PCR and RT-PCR

<400> 23
cttacccggg gacagggaca ag                                          22

<210> 24
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer HC FP for PCR and RT-PCR

<400> 24
tatacatatg gaagttcaat tagtagaaag cggcg                            35

<210> 25
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer HC RP for PCR and RT-PCR

<400> 25
ttttgtcgac cttacccggg gacagggaca ag                               32

<210> 26
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer Univ TolA RP for PCR and RT-PCR

<400> 26
ttcagttgcc gctttctttc                                             20

<210> 27
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer HER2 HCFAB Inner FP for PCR and RT-PCR

<400> 27
gagatatatc catggaagtt caattagtag aaagc                            35

<210> 28
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer HER2 HCFAB Inner RP for PCR and RT-PCR

<400> 28
gaggcgatat aaagcttaca agatttcgg                                   29

<210> 29
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer TolA Internal RP for PCR and RT-PCR

<400> 29
ctttggcggc ttctgctt                                               18

<210> 30
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer T7F for scFv VH library construction

<400> 30
atacgaaatt aatacgactc actataggga gaccacaacg g                     41

<210> 31
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer TolAR for scFv VH library construction

<400> 31
ttcagttgcc gctttctttc                                             20

<210> 32
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer TolAkR for scFv VH library construction

<400> 32
ccgcacacca gtaaggtgtg cggtttcagt tgccgctttc tttct                 45

<210> 33
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H1Fa for scFv VH library construction

<400> 33
cgcagcctct ggtttcaaca ttavtrrtwm ykmtatccac tgggtccgtc aggc       54

<210> 34
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H1Fb for scFv VH library construction

<400> 34
cgcagcctct ggtttcaaca ttavtrrtwm ykggatccac tgggtccgtc aggc       54

<210> 35
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H1Ra for scFv VH library construction

<400> 35
gcctgacgga cccagtggat akmrkwayya btaatgttga aaccagaggc tgcg       54

<210> 36
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H1Rb for scFv VH library construction

<400> 36
gcctgacgga cccagtggat ccmrkwayya btaatgttga aaccagaggc tgcg       54

<210> 37
<211> 76
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H2Fa for scFv VH library construction

<400> 37
gggtaaaggc ctggaatggg ttgstdggat tdmtccgdmt rrcggtdmta cgdactacgc 60
cgatagcgtg aaaggc                                                 76

<210> 38
<211> 76
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H2Fb for scFv VH library construction

<400> 38
gggtaaaggc ctggaatggg ttgstdhtat tdmtccgdmt rrcggtdmta cgdactacgc 60
cgatagcgtg aaaggc                                                 76

<210> 39
<211> 76
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H2Fc for scFv VH library construction

<400> 39
gggtaaaggc ctggaatggg ttgstgaaat tdmtccgdmt rrcggtdmta cgdactacgc 60
cgatagcgtg aaaggc                                                 76

<210> 40
<211> 76
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H2Ra for scFv VH library construction

<400> 40
gcctttcacg ctatcggcgt agthcgtakh accgyyakhc ggakhaatcc hascaaccca 60
ttccaggcct ttaccc                                                 76

<210> 41
<211> 76
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H2Rb for scFv VH library construction

<400> 41
gcctttcacg ctatcggcgt agthcgtakh accgyyakhc ggakhaatad hascaaccca 60
ttccaggcct ttaccc                                                 76

<210> 42
<211> 76
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H2Rc for scFv VH library construction

<400> 42
gcctttcacg ctatcggcgt agthcgtakh accgyyakhc ggakhaattt cascaaccca 60
ttccaggcct ttaccc                                                 76

<210> 43
<211> 79
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H3Fa for scFv VH library construction

<220> 
<221> modified_base  
<222> (1)...(79)
<223> n = g, a, c or t

<400> 43
cggaagatac cgccgtttat tactgtgcgc gcnnknnknn knnknnknnk nnknnkatgg 60
actactgggg tcagggcac                                              79

<210> 44
<211> 81
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H3Fb for scFv VH library
      construction

<220> 
<221> modified_base  
<222> (1)...(81)
<223> n = g, a, c or t

<400> 44
cggaagatac cgccgtttat tactgtgcgc gcnnknnknn knnknnknnk nnknnktttg 60
actactgggg tcagggcacc c                                           81

<210> 45
<211> 79
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H3Ra for scFv VH library construction

<220> 
<221> modified_base  
<222> (1)...(79)
<223> n = g, a, c or t

<400> 45
gtgccctgac cccagtagtc catmnnmnnm nnmnnmnnmn nmnnmnngcg cgcacagtaa 60
taaacggcgg tatcttccg                                              79

<210> 46
<211> 81
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer H3Rb for scFv VH library construction

<220> 
<221> modified_base  
<222> (1)...(81)
<223> n = g, a, c or t

<400> 46
gggtgccctg accccagtag tcaaamnnmn nmnnmnnmnn mnnmnnmnng cgcgcacagt 60
aataaacggc ggtatcttcc g                                           81

<210> 47
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer VLF for scFv VH library construction

<400> 47
gatatatcca tggatattca aatgacccaa tctc                             34

<210> 48
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer VHR for scFv VH library construction

<400> 48
cgatataaag cttggaactg acggtaacc                                   29

<210> 49
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer VLR for scFv VH library construction

<400> 49
gagattgggt catttgaata tccatggata tatc                             34

<210> 50
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer VHF for scFv VH library construction

<400> 50
ggttaccgtc agttccaagc tttatatcg                                   29

<210> 51
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer BsaI-VLF for scFv VH library construction

<400> 51
aaaaaggtct cctatggata ttcaaatgac ccaatctccg tcttc                 45

<210> 52
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer BsaI-VHR for scFv VH library construction

<400> 52
aaaaaaggtc tccccgcgga actgacggta accagggtgc c                     41


