                               SEQUENCE LISTING

<110> PRESIDENT AND FELLOWS OF HARVARD COLLEGE
 
<120> GENETIC REPROGRAMMING OF BACTERIAL BIOFILMS

<130> 002806-076751-PCT

<140> PCT/US14/35095
<141> 2014-04-23

<150> 61/814,908
<151> 2013-04-23

<160> 22    

<170> PatentIn version 3.5

<210> 1
<211> 151
<212> PRT
<213> Escherichia coli

<400> 1
Met Lys Leu Leu Lys Val Ala Ala Ile Ala Ala Ile Val Phe Ser Gly 
1               5                   10                  15      


Ser Ala Leu Ala Gly Val Val Pro Gln Tyr Gly Gly Gly Gly Asn His 
            20                  25                  30          


Gly Gly Gly Gly Asn Asn Ser Gly Pro Asn Ser Glu Leu Asn Ile Tyr 
        35                  40                  45              


Gln Tyr Gly Gly Gly Asn Ser Ala Leu Ala Leu Gln Thr Asp Ala Arg 
    50                  55                  60                  


Asn Ser Asp Leu Thr Ile Thr Gln His Gly Gly Gly Asn Gly Ala Asp 
65                  70                  75                  80  


Val Gly Gln Gly Ser Asp Asp Ser Ser Ile Asp Leu Thr Gln Arg Gly 
                85                  90                  95      


Phe Gly Asn Ser Ala Thr Leu Asp Gln Trp Asn Gly Lys Asn Ser Glu 
            100                 105                 110         


Met Thr Val Lys Gln Phe Gly Gly Gly Asn Gly Ala Ala Val Asp Gln 
        115                 120                 125             


Thr Ala Ser Asn Ser Ser Val Asn Val Thr Gln Val Gly Phe Gly Asn 
    130                 135                 140                 


Asn Ala Thr Ala His Gln Tyr 
145                 150     


<210> 2
<211> 513
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 2
atgaaacttt taaaagtagc agcaattgca gcaatcgtat tctccggtag cgctctggca       60

ggtgttgttc ctcagtacgg cggcggcggt aaccacggtg gtggcggtaa taatagcggc      120

ccaaattctg agctgaacat ttaccagtac ggtggcggta actctgcact tgctctgcaa      180

actgatgccc gtaactctga cttgactatt acccagcatg gcggcggtaa tggtgcagat      240

gttggtcagg gctcagatga cagctcaatc gatctgaccc aacgtggctt cggtaacagc      300

gctactcttg atcagtggaa cggcaaaaat tctgaaatga cggttaaaca gttcggtggt      360

ggcaacggtg ctgcagttga ccagactgca tctaactcct ccgtcaacgt gactcaggtt      420

ggctttggta acaacgcgac cgctcatcag tacggcagcg gtggttctgg cgcgcacatc      480

gttatggttg acgcgtacaa accgaccaaa tga                                   513


<210> 3
<211> 170
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
Met Lys Leu Leu Lys Val Ala Ala Ile Ala Ala Ile Val Phe Ser Gly 
1               5                   10                  15      


Ser Ala Leu Ala Gly Val Val Pro Gln Tyr Gly Gly Gly Gly Asn His 
            20                  25                  30          


Gly Gly Gly Gly Asn Asn Ser Gly Pro Asn Ser Glu Leu Asn Ile Tyr 
        35                  40                  45              


Gln Tyr Gly Gly Gly Asn Ser Ala Leu Ala Leu Gln Thr Asp Ala Arg 
    50                  55                  60                  


Asn Ser Asp Leu Thr Ile Thr Gln His Gly Gly Gly Asn Gly Ala Asp 
65                  70                  75                  80  


Val Gly Gln Gly Ser Asp Asp Ser Ser Ile Asp Leu Thr Gln Arg Gly 
                85                  90                  95      


Phe Gly Asn Ser Ala Thr Leu Asp Gln Trp Asn Gly Lys Asn Ser Glu 
            100                 105                 110         


Met Thr Val Lys Gln Phe Gly Gly Gly Asn Gly Ala Ala Val Asp Gln 
        115                 120                 125             


Thr Ala Ser Asn Ser Ser Val Asn Val Thr Gln Val Gly Phe Gly Asn 
    130                 135                 140                 


Asn Ala Thr Ala His Gln Tyr Gly Ser Gly Gly Ser Gly Ala His Ile 
145                 150                 155                 160 


Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
                165                 170 


<210> 4
<211> 1914
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 4
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60

atggcaaatc ttaatgggac gctgatgcag tattttgaat ggtacatgcc caatgacggc      120

caacattgga agcgcttgca aaacgactcg gcatatttgg ctgaacacgg tattactgcc      180

gtctggattc ccccggcata taagggaacg agccaagcgg atgtgggcta cggtgcttac      240

gacctttatg atttagggga gtttcatcaa aaagggacgg ttcggacaaa gtacggcaca      300

aaaggagagc tgcaatctgc gatcaaaagt cttcattccc gcgacattaa cgtttacggg      360

gatgtggtca tcaaccacaa aggcggcgct gatgcgaccg aagatgtaac cgcggttgaa      420

gtcgatcccg ctgaccgcaa ccgcgtaatt tcaggagaac acccaattaa agcctggaca      480

cattttcatt ttccggggcg cggcagcaca tacagcgatt ttaaatggca ttggtaccat      540

tttgacggaa ccgattggga cgagtcccga aagctgaacc gcatctataa gtttcaagga      600

aaggcttggg attgggaagt ttccaatgaa aacggcaact atgattattt gatgtatgcc      660

gacatcgatt atgaccatcc tgatgtcgca gcagaaatta agagatgggg cacttggtat      720

gccaatgaac tgcaattgga cggtttccgt cttgatgctg tcaaacacat taaattttct      780

tttttgcggg attgggttaa tcatgtcagg gaaaaaacgg ggaaggaaat gtttacggta      840

gctgaatatt ggcagaatga cttgggcgcg ctggaaaact atttgaacaa aacaaatttt      900

aatcattcag tgtttgacgt gccgcttcat tatcagttcc atgctgcatc gacacaggga      960

ggcggctatg atatgaggaa attgctgaac ggtacggtcg tttccaagca tccgttgaaa     1020

tcggttacat ttgtcgataa ccatgataca cagccggggc aatcgcttga gtcgactgtc     1080

caaacatggt ttaagccgct tgcttacgct tttattctca caagggaatc tggataccct     1140

caggttttct acggggatat gtacgggacg aaaggagact cccagcgcga aattcctgcc     1200

ttgaaacaca aaattgaacc gatcttaaaa gcgagaaaac agtatgcgta cggagcacag     1260

catgattatt tcgaccacca tgacattgtc ggctggacaa gggaaggcga cagctcggtt     1320

gcaaattcag gtttggcggc attaataaca gacggacccg gtggggcaaa gcgaatgtat     1380

gtcggccggc aaaacgccgg tgagacatgg catgacatta ccggaaaccg ttcggagccg     1440

gttgtcatca attcggaagg ctggggagag tttcacgtaa acggcgggtc ggtttcaatt     1500

tatgttcaaa gaggcggcgg ttctgattac gacatcccaa cgaccgaaaa cctgtatttt     1560

cagggcgcca tggttgatac cttatcaggt ttatcaagtg agcaaggtca gtccggtgat     1620

atgacaattg aagaagatag tgctacccat attaaattct caaaacgtga tgaggacggc     1680

aaagagttag ctggtgcaac tatggagttg cgtgattcat ctggtaaaac tattagtaca     1740

tggatttcag atggacaagt gaaagatttc tacctgtatc caggaaaata tacatttgtc     1800

gaaaccgcag caccagacgg ttatgaggta gcaactgcta ttacctttac agttaatgag     1860

caaggtcagg ttactgtaaa tggcaaagca actaaaggtg acgctcatat ttaa           1914


<210> 5
<211> 637
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 5
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Ala Asn Leu Asn Gly Thr Leu Met Gln Tyr Phe 
            20                  25                  30          


Glu Trp Tyr Met Pro Asn Asp Gly Gln His Trp Lys Arg Leu Gln Asn 
        35                  40                  45              


Asp Ser Ala Tyr Leu Ala Glu His Gly Ile Thr Ala Val Trp Ile Pro 
    50                  55                  60                  


Pro Ala Tyr Lys Gly Thr Ser Gln Ala Asp Val Gly Tyr Gly Ala Tyr 
65                  70                  75                  80  


Asp Leu Tyr Asp Leu Gly Glu Phe His Gln Lys Gly Thr Val Arg Thr 
                85                  90                  95      


Lys Tyr Gly Thr Lys Gly Glu Leu Gln Ser Ala Ile Lys Ser Leu His 
            100                 105                 110         


Ser Arg Asp Ile Asn Val Tyr Gly Asp Val Val Ile Asn His Lys Gly 
        115                 120                 125             


Gly Ala Asp Ala Thr Glu Asp Val Thr Ala Val Glu Val Asp Pro Ala 
    130                 135                 140                 


Asp Arg Asn Arg Val Ile Ser Gly Glu His Pro Ile Lys Ala Trp Thr 
145                 150                 155                 160 


His Phe His Phe Pro Gly Arg Gly Ser Thr Tyr Ser Asp Phe Lys Trp 
                165                 170                 175     


His Trp Tyr His Phe Asp Gly Thr Asp Trp Asp Glu Ser Arg Lys Leu 
            180                 185                 190         


Asn Arg Ile Tyr Lys Phe Gln Gly Lys Ala Trp Asp Trp Glu Val Ser 
        195                 200                 205             


Asn Glu Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Tyr 
    210                 215                 220                 


Asp His Pro Asp Val Ala Ala Glu Ile Lys Arg Trp Gly Thr Trp Tyr 
225                 230                 235                 240 


Ala Asn Glu Leu Gln Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His 
                245                 250                 255     


Ile Lys Phe Ser Phe Leu Arg Asp Trp Val Asn His Val Arg Glu Lys 
            260                 265                 270         


Thr Gly Lys Glu Met Phe Thr Val Ala Glu Tyr Trp Gln Asn Asp Leu 
        275                 280                 285             


Gly Ala Leu Glu Asn Tyr Leu Asn Lys Thr Asn Phe Asn His Ser Val 
    290                 295                 300                 


Phe Asp Val Pro Leu His Tyr Gln Phe His Ala Ala Ser Thr Gln Gly 
305                 310                 315                 320 


Gly Gly Tyr Asp Met Arg Lys Leu Leu Asn Gly Thr Val Val Ser Lys 
                325                 330                 335     


His Pro Leu Lys Ser Val Thr Phe Val Asp Asn His Asp Thr Gln Pro 
            340                 345                 350         


Gly Gln Ser Leu Glu Ser Thr Val Gln Thr Trp Phe Lys Pro Leu Ala 
        355                 360                 365             


Tyr Ala Phe Ile Leu Thr Arg Glu Ser Gly Tyr Pro Gln Val Phe Tyr 
    370                 375                 380                 


Gly Asp Met Tyr Gly Thr Lys Gly Asp Ser Gln Arg Glu Ile Pro Ala 
385                 390                 395                 400 


Leu Lys His Lys Ile Glu Pro Ile Leu Lys Ala Arg Lys Gln Tyr Ala 
                405                 410                 415     


Tyr Gly Ala Gln His Asp Tyr Phe Asp His His Asp Ile Val Gly Trp 
            420                 425                 430         


Thr Arg Glu Gly Asp Ser Ser Val Ala Asn Ser Gly Leu Ala Ala Leu 
        435                 440                 445             


Ile Thr Asp Gly Pro Gly Gly Ala Lys Arg Met Tyr Val Gly Arg Gln 
    450                 455                 460                 


Asn Ala Gly Glu Thr Trp His Asp Ile Thr Gly Asn Arg Ser Glu Pro 
465                 470                 475                 480 


Val Val Ile Asn Ser Glu Gly Trp Gly Glu Phe His Val Asn Gly Gly 
                485                 490                 495     


Ser Val Ser Ile Tyr Val Gln Arg Gly Gly Gly Ser Asp Tyr Asp Ile 
            500                 505                 510         


Pro Thr Thr Glu Asn Leu Tyr Phe Gln Gly Ala Met Val Asp Thr Leu 
        515                 520                 525             


Ser Gly Leu Ser Ser Glu Gln Gly Gln Ser Gly Asp Met Thr Ile Glu 
    530                 535                 540                 


Glu Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly 
545                 550                 555                 560 


Lys Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys 
                565                 570                 575     


Thr Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu 
            580                 585                 590         


Tyr Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr 
        595                 600                 605             


Glu Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val 
    610                 615                 620                 


Thr Val Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile 
625                 630                 635         


<210> 6
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (3)..(3)
<223> Any amino acid

<400> 6
Leu Pro Xaa Thr Gly 
1               5   


<210> 7
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 7
Gly Ser Gly Gly Ser Gly 
1               5       


<210> 8
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 8
His His His His His His 
1               5       


<210> 9
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Glu Pro Leu Gln Leu Lys Met 
1               5           


<210> 10
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 10
Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210> 11
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 11
His Ser Ser Tyr Trp Tyr Ala Phe Asn Asn Lys Thr 
1               5                   10          


<210> 12
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 12
Ala Tyr Ser Ser Gly Ala Pro Pro Met Pro Pro Phe 
1               5                   10          


<210> 13
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 13
Leu Arg Arg Ser Ser Glu Ala His Asn Ser Ile Val 
1               5                   10          


<210> 14
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 14
Pro Trp Ile Pro Thr Pro Arg Pro Thr Phe Thr Gly 
1               5                   10          


<210> 15
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 15
Pro Pro Pro Trp Leu Pro Tyr Met Pro Pro Trp Ser 
1               5                   10          


<210> 16
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 16
Asn Pro Tyr His Pro Thr Ile Pro Gln Ser Val His 
1               5                   10          


<210> 17
<211> 13
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 17
Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
1               5                   10              


<210> 18
<211> 18
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 18
Cys Gly Pro Ala Gly Asp Ser Ser Gly Val Asp Ser Arg Ser Val Gly 
1               5                   10                  15      


Pro Cys 
        


<210> 19
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 19
Lys Cys Thr Ser Asp Gln Asp Glu Gln Phe Ile Pro Lys Gly Cys Ser 
1               5                   10                  15      


Lys Gly Ser Gly Gly Ser Gly 
            20              


<210> 20
<211> 37
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 20
Asp Thr Ala Ser Asp Ala Ala Ala Ala Ala Ala Leu Thr Ala Ala Asn 
1               5                   10                  15      


Ala Lys Ala Ala Ala Glu Leu Thr Ala Ala Asn Ala Ala Ala Ala Ala 
            20                  25                  30          


Ala Ala Thr Ala Arg 
        35          


<210> 21
<211> 59
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 21
Gly Gly Thr Ile Trp Thr Gly Lys Gly Leu Gly Leu Gly Leu Gly Leu 
1               5                   10                  15      


Gly Leu Gly Ala Trp Gly Pro Ile Ile Leu Gly Val Val Gly Ala Gly 
            20                  25                  30          


Ala Val Tyr Ala Tyr Met Lys Ser Arg Asp Ile Glu Ser Ala Gln Ser 
        35                  40                  45              


Asp Glu Glu Val Glu Leu Arg Asp Ala Leu Ala 
    50                  55                  


<210> 22
<211> 17
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 22
Lys Cys Thr Ser Asp Gln Asp Glu Gln Phe Ile Pro Lys Gly Cys Ser 
1               5                   10                  15      


Lys 
    


