                               SEQUENCE LISTING

<110> AMIDEBIO, LLC
 
<120> CHEMICALLY AND THERMODYNAMICALLY STABLE INSULIN ANALOGUES AND 
      IMPROVED METHODS FOR THEIR PRODUCTION

<130> 39538-706.602

<140> PCT/US2014/034411
<141> 2014-04-16

<150> 61/813,174
<151> 2013-04-17

<160> 39    

<170> PatentIn version 3.5

<210> 1
<211> 31
<212> PRT
<213> Homo sapiens

<400> 1
Glu Ala Glu Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly Gly Pro 
1               5                   10                  15      


Gly Ala Gly Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu Gln 
            20                  25                  30      


<210> 2
<211> 20
<212> PRT
<213> Homo sapiens

<400> 2
Gly Ile Val Glu Gln Cys Cys Ser Ile Cys Ser Leu Tyr Gln Leu Glu 
1               5                   10                  15      


Asn Tyr Cys Asn 
            20  


<210> 3
<211> 30
<212> PRT
<213> Homo sapiens

<400> 3
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210> 4
<211> 58
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (1)..(2) 
<223> May or may not be present

<220>
<221> MOD_RES
<222> (3)..(3) 
<223> Asn, Ala, Lys, Asp or absent

<220>
<221> MOD_RES
<222> (31)..(32) 
<223> May or may not be present

<220>
<221> MOD_RES
<222> (33)..(33) 
<223> Tyr or Val

<220>
<221> MOD_RES
<222> (34)..(34) 
<223> May or may not be present

<220>
<221> MOD_RES
<222> (35)..(35) 
<223> Gly or Asp

<220>
<221> MOD_RES
<222> (36)..(36) 
<223> Asp or Gly

<220>
<221> MOD_RES
<222> (37)..(37) 
<223> Val or Lys

<220>
<221> MOD_RES
<222> (58)..(58)
<223> Asn, Gly, Ala, Asp or Gln

<400> 4
Phe Val Xaa Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg 
            20                  25                  30          


Xaa Pro Xaa Xaa Xaa Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys 
        35                  40                  45              


Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Xaa 
    50                  55              


<210> 5
<211> 58
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 5
Met Thr Thr Thr Tyr Asp Arg Arg Phe Thr Thr Thr Gln Pro Arg Tyr 
1               5                   10                  15      


Arg Gln Asp Asp Arg Ser Arg Tyr Asp Gln Gln Thr Arg Ser Gln Ser 
            20                  25                  30          


Thr Ser Arg Thr Leu Ala Ile Ile Ala Leu Leu Pro Val Gly Gly Ile 
        35                  40                  45              


Leu Leu Gly Leu Arg Asn Arg Gly Ile Thr 
    50                  55              


<210> 6
<211> 58
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 6
Met Thr Thr Thr Tyr Asp Arg Arg Phe Thr Thr Thr Gln Pro Arg Tyr 
1               5                   10                  15      


Arg Gln Asp Asp Arg Ser Arg Tyr Asp Gln Gln Thr Arg Ser Gln Ser 
            20                  25                  30          


Thr Ser Arg Thr Leu Ala Ile Ile Ala Leu Leu Pro Val Gly Gly Ile 
        35                  40                  45              


Leu Leu Gly Leu Arg Asn Arg Gly Ile Thr 
    50                  55              


<210> 7
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 7
Met Pro Ser Asp Lys Pro His His Lys Lys His His Lys Lys His His 
1               5                   10                  15      


Ser Ser Gly Ser 
            20  


<210> 8
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 8
Gly Glu Glu Thr Leu Trp 
1               5       


<210> 9
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Asn Lys Gly Ile Thr Trp 
1               5       


<210> 10
<211> 58
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 10
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg 
            20                  25                  30          


Tyr Pro Gly Asp Val Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys 
        35                  40                  45              


Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Gly 
    50                  55              


<210> 11
<211> 58
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 11
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg 
            20                  25                  30          


Tyr Pro Asp Gly Val Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys 
        35                  40                  45              


Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Gly 
    50                  55              


<210> 12
<211> 57
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 12
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Tyr 
            20                  25                  30          


Pro Asp Gly Val Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser 
        35                  40                  45              


Leu Tyr Gln Leu Glu Asn Tyr Cys Gly 
    50                  55          


<210> 13
<211> 56
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 13
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Val 
            20                  25                  30          


Asp Gly Val Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu 
        35                  40                  45              


Tyr Gln Leu Glu Asn Tyr Cys Gly 
    50                  55      


<210> 14
<211> 55
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 14
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Val Asp 
            20                  25                  30          


Gly Lys Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
        35                  40                  45              


Gln Leu Glu Asn Tyr Cys Gly 
    50                  55  


<210> 15
<211> 55
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 15
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Tyr Asp 
            20                  25                  30          


Gly Lys Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr 
        35                  40                  45              


Gln Leu Glu Asn Tyr Cys Gly 
    50                  55  


<210> 16
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(2) 
<223> May or may not be present

<220>
<221> MOD_RES
<222> (3)..(3) 
<223> Tyr or Val

<220>
<221> MOD_RES
<222> (4)..(4) 
<223> May or may not be present

<220>
<221> MOD_RES
<222> (5)..(5) 
<223> Gly or Asp

<220>
<221> MOD_RES
<222> (6)..(6) 
<223> Asp or Gly

<220>
<221> MOD_RES
<222> (7)..(7) 
<223> Val or Lys

<400> 16
Arg Arg Xaa Pro Xaa Xaa Xaa 
1               5           


<210> 17
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(2) 
<223> May or may not be present

<220>
<221> MOD_RES
<222> (3)..(3) 
<223> Tyr or Val

<220>
<221> MOD_RES
<222> (4)..(4) 
<223> May or may not be present

<220>
<221> MOD_RES
<222> (7)..(7) 
<223> Val or Lys

<400> 17
Arg Arg Xaa Pro Gly Asp Xaa 
1               5           


<210> 18
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(2) 
<223> May or may not be present

<220>
<221> MOD_RES
<222> (3)..(3) 
<223> Tyr or Val

<220>
<221> MOD_RES
<222> (4)..(4) 
<223> May or may not be present

<220>
<221> MOD_RES
<222> (7)..(7) 
<223> Val or Lys

<400> 18
Arg Arg Xaa Pro Asp Gly Xaa 
1               5           


<210> 19
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(2) 
<223> May or may not be present

<220>
<221> MOD_RES
<222> (3)..(3) 
<223> Tyr or Val

<220>
<221> MOD_RES
<222> (4)..(4) 
<223> May or may not be present

<400> 19
Arg Arg Xaa Pro Asp Gly Val 
1               5           


<210> 20
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(2) 
<223> May or may not be present

<220>
<221> MOD_RES
<222> (3)..(3) 
<223> Tyr or Val

<220>
<221> MOD_RES
<222> (4)..(4) 
<223> May or may not be present

<400> 20
Arg Arg Xaa Pro Asp Gly Lys 
1               5           


<210> 21
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 21
Arg Arg Tyr Pro Gly Asp Val 
1               5           


<210> 22
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 22
Arg Arg Tyr Pro Asp Gly Val 
1               5           


<210> 23
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 23
Arg Arg Val Asp Gly Val 
1               5       


<210> 24
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 24
Arg Tyr Pro Asp Gly Val 
1               5       


<210> 25
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 25
Arg Val Asp Gly Val 
1               5   


<210> 26
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 26
Val Asp Gly Lys 
1               


<210> 27
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 27
Tyr Asp Gly Lys 
1               


<210> 28
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(2) 
<223> May or may not be present

<220>
<221> MOD_RES
<222> (3)..(3) 
<223> Tyr or Val

<220>
<221> MOD_RES
<222> (4)..(4) 
<223> May or may not be present

<220>
<221> MOD_RES
<222> (5)..(5) 
<223> Gly or Asp

<220>
<221> MOD_RES
<222> (6)..(6) 
<223> Asp or Gly

<220>
<221> MOD_RES
<222> (7)..(7) 
<223> Any amino acid

<400> 28
Arg Arg Xaa Pro Xaa Xaa Xaa 
1               5           


<210> 29
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (5)..(5)
<223> Any amino acid

<400> 29
Tyr Pro Gly Asp Xaa 
1               5   


<210> 30
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> misc_feature
<222> (1)..(10)
<223> This sequence may encompass 5-10 "Lys" residues, wherein
      some positions may be absent

<400> 30
Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys 
1               5                   10  


<210> 31
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> misc_feature
<222> (1)..(10)
<223> This sequence may encompass 5-10 "His" residues, wherein
      some positions may be absent

<400> 31
His His His His His His His His His His 
1               5                   10  


<210> 32
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> misc_feature
<222> (1)..(10)
<223> This sequence may encompass 5-10 "Glu" residues, wherein
      some positions may be absent

<400> 32
Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu 
1               5                   10  


<210> 33
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> misc_feature
<222> (1)..(10)
<223> This sequence may encompass 5-10 "Arg" residues, wherein
      some positions may be absent

<400> 33
Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg 
1               5                   10  


<210> 34
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 34
His His His His His His 
1               5       


<210> 35
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xLys tag

<400> 35
Lys Lys Lys Lys Lys Lys 
1               5       


<210> 36
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xGlu tag

<400> 36
Glu Glu Glu Glu Glu Glu 
1               5       


<210> 37
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xArg tag

<400> 37
Arg Arg Arg Arg Arg Arg 
1               5       


<210> 38
<211> 21
<212> PRT
<213> Homo sapiens

<400> 38
Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210> 39
<211> 30
<212> PRT
<213> Homo sapiens

<400> 39
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  



