
                                SEQUENCE LISTING

<110> Whitehead Institute for Biomedical Research

<120> Methods Of Mutating, Modifying Or
  Modulating Nucleic Acid In A Cell Or Nonhuman Mammal
  

<130> 0399.2052-004

<140> PCT/US2014/034387   
<141> 2014-04-16  

<150> US 61/812,720       
<151> 2013-04-16  

<150> US 61/824,920       
<151> 2013-05-17  

<150> US 61/858,437       
<151> 2013-07-25  

<150> US 61/865,888       
<151> 2013-08-14  

<160> 488

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1441
<212> PRT
<213> Unknown

<220> 
<223> dCas9TA

<400> 1
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Asp Ala Leu Asp Asp Phe
        1395                1400                1405            
Asp Leu Asp Met Leu Pro Ala Asp Ala Leu Asp Asp Phe Asp Leu Asp
    1410                1415                1420                
Met Leu Pro Ala Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Pro
1425                1430                1435               1440 
Gly
    


<210> 2
<211> 1631
<212> PRT
<213> Unknown

<220> 
<223> dCas9Apobec1

<400> 2
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Ser Ser Glu Thr Gly
        1395                1400                1405            
Pro Val Ala Val Asp Pro Thr Leu Arg Arg Arg Ile Glu Pro His Glu
    1410                1415                1420                
Phe Glu Val Phe Phe Asp Pro Arg Glu Leu Arg Lys Glu Thr Cys Leu
1425                1430                1435               1440 
Leu Tyr Glu Ile Asn Trp Gly Gly Arg His Ser Val Trp Arg His Thr
                1445                1450                1455    
Ser Gln Asn Thr Ser Asn His Val Glu Val Asn Phe Leu Glu Lys Phe
            1460                1465                1470        
Thr Thr Glu Arg Tyr Phe Arg Pro Asn Thr Arg Cys Ser Ile Thr Trp
        1475                1480                1485            
Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys Ser Arg Ala Ile Thr Glu
    1490                1495                1500                
Phe Leu Ser Arg His Pro Tyr Val Thr Leu Phe Ile Tyr Ile Ala Arg
1505                1510                1515               1520 
Leu Tyr His His Thr Asp Gln Arg Asn Arg Gln Gly Leu Arg Asp Leu
                1525                1530                1535    
Ile Ser Ser Gly Val Thr Ile Gln Ile Met Thr Glu Gln Glu Tyr Cys
            1540                1545                1550        
Tyr Cys Trp Arg Asn Phe Val Asn Tyr Pro Pro Ser Asn Glu Ala Tyr
        1555                1560                1565            
Trp Pro Arg Tyr Pro His Leu Trp Val Lys Leu Tyr Val Leu Glu Leu
    1570                1575                1580                
Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys Leu Lys Ile Leu Arg Arg
1585                1590                1595               1600 
Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile Thr Leu Gln Thr Cys His
                1605                1610                1615    
Tyr Gln Arg Ile Pro Pro His Leu Leu Trp Ala Thr Gly Leu Lys
            1620                1625                1630    


<210> 3
<211> 1774
<212> PRT
<213> Unknown

<220> 
<223> dCas9Cdk9

<400> 3
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Ala Lys Gln Tyr Asp
        1395                1400                1405            
Ser Val Glu Cys Pro Phe Cys Asp Glu Val Thr Lys Tyr Glu Lys Leu
    1410                1415                1420                
Ala Lys Ile Gly Gln Gly Thr Phe Gly Glu Val Phe Lys Ala Lys His
1425                1430                1435               1440 
Arg Gln Thr Gly Gln Lys Val Ala Leu Lys Lys Val Leu Met Glu Asn
                1445                1450                1455    
Glu Lys Glu Gly Phe Pro Ile Thr Ala Leu Arg Glu Ile Lys Ile Leu
            1460                1465                1470        
Gln Leu Leu Lys His Glu Asn Val Val Asn Leu Ile Glu Ile Cys Arg
        1475                1480                1485            
Thr Lys Ala Ser Pro Tyr Asn Arg Cys Lys Gly Ser Ile Tyr Leu Val
    1490                1495                1500                
Phe Asp Phe Cys Glu His Asp Leu Ala Gly Leu Leu Ser Asn Val Leu
1505                1510                1515               1520 
Val Lys Phe Thr Leu Ser Glu Ile Lys Arg Val Met Gln Met Leu Leu
                1525                1530                1535    
Asn Gly Leu Tyr Tyr Ile His Arg Asn Lys Ile Leu His Arg Asp Met
            1540                1545                1550        
Lys Ala Ala Asn Val Leu Ile Thr Arg Asp Gly Val Leu Lys Leu Ala
        1555                1560                1565            
Asp Phe Gly Leu Ala Arg Ala Phe Ser Leu Ala Lys Asn Ser Gln Pro
    1570                1575                1580                
Asn Arg Tyr Thr Asn Arg Val Val Thr Leu Trp Tyr Arg Pro Pro Glu
1585                1590                1595               1600 
Leu Leu Leu Gly Glu Arg Asp Tyr Gly Pro Pro Ile Asp Leu Trp Gly
                1605                1610                1615    
Ala Gly Cys Ile Met Ala Glu Met Trp Thr Arg Ser Pro Ile Met Gln
            1620                1625                1630        
Gly Asn Thr Glu Gln His Gln Leu Ala Leu Ile Ser Gln Leu Cys Gly
        1635                1640                1645            
Ser Ile Thr Pro Glu Val Trp Pro Asn Val Asp Lys Tyr Glu Leu Phe
    1650                1655                1660                
Glu Lys Leu Glu Leu Val Lys Gly Gln Lys Arg Lys Val Lys Asp Arg
1665                1670                1675               1680 
Leu Lys Ala Tyr Val Arg Asp Pro Tyr Ala Leu Asp Leu Ile Asp Lys
                1685                1690                1695    
Leu Leu Val Leu Asp Pro Ala Gln Arg Ile Asp Ser Asp Asp Ala Leu
            1700                1705                1710        
Asn His Asp Phe Phe Trp Ser Asp Pro Met Pro Ser Asp Leu Lys Gly
        1715                1720                1725            
Met Leu Ser Thr His Leu Thr Ser Met Phe Glu Tyr Leu Ala Pro Pro
    1730                1735                1740                
Arg Arg Lys Gly Ser Gln Ile Thr Gln Gln Ser Thr Asn Gln Ser Arg
1745                1750                1755               1760 
Asn Pro Ala Thr Thr Asn Gln Thr Glu Phe Glu Arg Val Phe
                1765                1770                


<210> 4
<211> 2126
<212> PRT
<213> Unknown

<220> 
<223> dCas9CycT

<400> 4
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Glu Gly Glu Arg Lys
        1395                1400                1405            
Asn Asn Asn Lys Arg Trp Tyr Phe Thr Arg Glu Gln Leu Glu Asn Ser
    1410                1415                1420                
Pro Ser Arg Arg Phe Gly Val Asp Ser Asp Lys Glu Leu Ser Tyr Arg
1425                1430                1435               1440 
Gln Gln Ala Ala Asn Leu Leu Gln Asp Met Gly Gln Arg Leu Asn Val
                1445                1450                1455    
Ser Gln Leu Thr Ile Asn Thr Ala Ile Val Tyr Met His Arg Phe Tyr
            1460                1465                1470        
Met Ile Gln Ser Phe Thr Gln Phe His Arg Tyr Ser Met Ala Pro Ala
        1475                1480                1485            
Ala Leu Phe Leu Ala Ala Lys Val Glu Glu Gln Pro Lys Lys Leu Glu
    1490                1495                1500                
His Val Ile Lys Val Ala His Thr Cys Leu His Pro Gln Glu Ser Leu
1505                1510                1515               1520 
Pro Asp Thr Arg Ser Glu Ala Tyr Leu Gln Gln Val Gln Asp Leu Val
                1525                1530                1535    
Ile Leu Glu Ser Ile Ile Leu Gln Thr Leu Gly Phe Glu Leu Thr Ile
            1540                1545                1550        
Asp His Pro His Thr His Val Val Lys Cys Thr Gln Leu Val Arg Ala
        1555                1560                1565            
Ser Lys Asp Leu Ala Gln Thr Ser Tyr Phe Met Ala Thr Asn Ser Leu
    1570                1575                1580                
His Leu Thr Thr Phe Ser Leu Gln Tyr Thr Pro Pro Val Val Ala Cys
1585                1590                1595               1600 
Val Cys Ile His Leu Ala Cys Lys Trp Ser Asn Trp Glu Ile Pro Val
                1605                1610                1615    
Ser Thr Asp Gly Lys His Trp Trp Glu Tyr Val Asp Ala Thr Val Thr
            1620                1625                1630        
Leu Glu Leu Leu Asp Glu Leu Thr His Glu Phe Leu Gln Ile Leu Glu
        1635                1640                1645            
Lys Thr Pro Ser Arg Leu Lys Arg Ile Arg Asn Trp Arg Ala Tyr Gln
    1650                1655                1660                
Ala Ala Met Lys Thr Lys Pro Asp Asp Arg Gly Ala Asp Glu Asn Thr
1665                1670                1675               1680 
Ser Glu Gln Thr Ile Leu Asn Met Ile Ser Gln Thr Ser Ser Asp Thr
                1685                1690                1695    
Thr Ile Ala Gly Leu Met Ser Met Ser Thr Ala Ser Thr Ser Ala Val
            1700                1705                1710        
Pro Ser Leu Pro Ser Ser Glu Glu Ser Ser Ser Ser Leu Thr Ser Val
        1715                1720                1725            
Asp Met Leu Gln Gly Glu Arg Trp Leu Ser Ser Gln Pro Pro Phe Lys
    1730                1735                1740                
Leu Glu Ala Ala Gln Gly His Arg Thr Ser Glu Ser Leu Ala Leu Ile
1745                1750                1755               1760 
Gly Val Asp His Ser Leu Gln Gln Asp Gly Ser Ser Ala Phe Gly Ser
                1765                1770                1775    
Gln Lys Gln Ala Ser Lys Ser Val Pro Ser Ala Lys Val Ser Leu Lys
            1780                1785                1790        
Glu Tyr Arg Ala Lys His Ala Glu Glu Leu Ala Ala Gln Lys Arg Gln
        1795                1800                1805            
Leu Glu Asn Met Glu Ala Asn Val Lys Ser Gln Tyr Ala Tyr Ala Ala
    1810                1815                1820                
Gln Asn Leu Leu Ser His Asp Ser His Ser Ser Val Ile Leu Lys Met
1825                1830                1835               1840 
Pro Ile Glu Ser Ser Glu Asn Pro Glu Arg Pro Phe Leu Asp Lys Ala
                1845                1850                1855    
Asp Lys Ser Ala Leu Lys Met Arg Leu Pro Val Ala Ser Gly Asp Lys
            1860                1865                1870        
Ala Val Ser Ser Lys Pro Glu Glu Ile Lys Met Arg Ile Lys Val His
        1875                1880                1885            
Ser Ala Gly Asp Lys His Asn Ser Ile Glu Asp Ser Val Thr Lys Ser
    1890                1895                1900                
Arg Glu His Lys Glu Lys Gln Arg Thr His Pro Ser Asn His His His
1905                1910                1915               1920 
His His Asn His His Ser His Arg His Ser His Leu Gln Leu Pro Ala
                1925                1930                1935    
Gly Pro Val Ser Lys Arg Pro Ser Asp Pro Lys His Ser Ser Gln Thr
            1940                1945                1950        
Ser Thr Leu Ala His Lys Thr Tyr Ser Leu Ser Ser Thr Leu Ser Ser
        1955                1960                1965            
Ser Ser Ser Thr Arg Lys Arg Gly Pro Pro Glu Glu Thr Gly Ala Ala
    1970                1975                1980                
Val Phe Asp His Pro Ala Lys Ile Ala Lys Ser Thr Lys Ser Ser Leu
1985                1990                1995               2000 
Asn Phe Pro Phe Pro Pro Leu Pro Thr Met Thr Gln Leu Pro Gly His
                2005                2010                2015    
Ser Ser Asp Thr Ser Gly Leu Pro Phe Ser Gln Pro Ser Cys Lys Thr
            2020                2025                2030        
Arg Val Pro His Met Lys Leu Asp Lys Gly Pro Pro Gly Ala Asn Gly
        2035                2040                2045            
His Asn Ala Thr Gln Ser Ile Asp Tyr Gln Asp Thr Val Asn Met Leu
    2050                2055                2060                
His Ser Leu Leu Ser Ala Gln Gly Val Gln Pro Thr Gln Ala Pro Ala
2065                2070                2075               2080 
Phe Glu Phe Val His Ser Tyr Gly Glu Tyr Met Asn Pro Arg Ala Gly
                2085                2090                2095    
Ala Ile Ser Ser Arg Ser Gly Thr Thr Asp Lys Pro Arg Pro Pro Pro
            2100                2105                2110        
Leu Pro Ser Glu Pro Pro Pro Pro Leu Pro Pro Leu Pro Lys
        2115                2120                2125    


<210> 5
<211> 1714
<212> PRT
<213> Unknown

<220> 
<223> dCas9EnR

<400> 5
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Leu Glu Asp Arg Cys Ser
        1395                1400                1405            
Pro Gln Ser Ala Pro Ser Pro Ile Thr Leu Gln Met Gln His Leu His
    1410                1415                1420                
His Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Met Gln His Leu
1425                1430                1435               1440 
His Gln Leu Gln Gln Leu Gln Gln Leu His Gln Gln Gln Leu Ala Ala
                1445                1450                1455    
Gly Val Phe His His Pro Ala Met Ala Phe Asp Ala Ala Ala Ala Ala
            1460                1465                1470        
Ala Ala Ala Ala Ala Ala Ala Ala Ala His Ala His Ala Ala Ala Leu
        1475                1480                1485            
Gln Gln Arg Leu Ser Gly Ser Gly Ser Pro Ala Ser Cys Ser Thr Pro
    1490                1495                1500                
Ala Ser Ser Thr Pro Leu Thr Ile Lys Glu Glu Glu Ser Asp Ser Val
1505                1510                1515               1520 
Ile Gly Asp Met Ser Phe His Asn Gln Thr His Thr Thr Asn Glu Glu
                1525                1530                1535    
Glu Glu Ala Glu Glu Asp Asp Asp Ile Asp Val Asp Val Asp Asp Thr
            1540                1545                1550        
Ser Ala Gly Gly Arg Leu Pro Pro Pro Ala His Gln Gln Gln Ser Thr
        1555                1560                1565            
Ala Lys Pro Ser Leu Ala Phe Ser Ile Ser Asn Ile Leu Ser Asp Arg
    1570                1575                1580                
Phe Gly Asp Val Gln Lys Pro Gly Lys Ser Ile Glu Asn Gln Ala Ser
1585                1590                1595               1600 
Ile Phe Arg Pro Phe Glu Ala Asn Arg Ser Gln Thr Ala Thr Pro Ser
                1605                1610                1615    
Ala Phe Thr Arg Val Asp Leu Leu Glu Phe Ser Arg Gln Gln Gln Ala
            1620                1625                1630        
Ala Ala Ala Ala Ala Thr Ala Ala Met Met Leu Glu Arg Ala Asn Phe
        1635                1640                1645            
Leu Asn Cys Phe Asn Pro Ala Ala Tyr Pro Arg Ile His Glu Glu Ile
    1650                1655                1660                
Val Gln Ser Arg Leu Arg Arg Ser Ala Ala Asn Ala Val Ile Pro Pro
1665                1670                1675               1680 
Pro Met Ser Ser Lys Met Ser Asp Ala Asn Pro Glu Lys Ser Ala Leu
                1685                1690                1695    
Gly Ser Met Gln Pro Lys Leu Glu Gln Lys Leu Ile Ser Glu Glu Asp
            1700                1705                1710        
Leu Asn
        


<210> 6
<211> 1567
<212> PRT
<213> Unknown

<220> 
<223> dCas9Gadd45a

<400> 6
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Thr Leu Glu Glu Phe
        1395                1400                1405            
Ser Ala Ala Glu Gln Lys Thr Glu Arg Met Asp Thr Val Gly Asp Ala
    1410                1415                1420                
Leu Glu Glu Val Leu Ser Lys Ala Arg Ser Gln Arg Thr Ile Thr Val
1425                1430                1435               1440 
Gly Val Tyr Glu Ala Ala Lys Leu Leu Asn Val Asp Pro Asp Asn Val
                1445                1450                1455    
Val Leu Cys Leu Leu Ala Ala Asp Glu Asp Asp Asp Arg Asp Val Ala
            1460                1465                1470        
Leu Gln Ile His Phe Thr Leu Ile Arg Ala Phe Cys Cys Glu Asn Asp
        1475                1480                1485            
Ile Asn Ile Leu Arg Val Ser Asn Pro Gly Arg Leu Ala Glu Leu Leu
    1490                1495                1500                
Leu Leu Glu Asn Asp Ala Gly Pro Ala Glu Ser Gly Gly Ala Ala Gln
1505                1510                1515               1520 
Thr Pro Asp Leu His Cys Val Leu Val Thr Asn Pro His Ser Ser Gln
                1525                1530                1535    
Trp Lys Asp Pro Ala Leu Ser Gln Leu Ile Cys Phe Cys Arg Glu Ser
            1540                1545                1550        
Arg Tyr Met Asp Gln Trp Val Pro Val Ile Asn Leu Pro Glu Arg
        1555                1560                1565        


<210> 7
<211> 2044
<212> PRT
<213> Unknown

<220> 
<223> dCas9p300HAT

<400> 7
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Tyr Arg Gln Asp Pro Glu
        1395                1400                1405            
Ser Leu Pro Phe Arg Gln Pro Val Asp Pro Gln Leu Leu Gly Ile Pro
    1410                1415                1420                
Asp Tyr Phe Asp Ile Val Lys Ser Pro Met Asp Leu Ser Thr Ile Lys
1425                1430                1435               1440 
Arg Lys Leu Asp Thr Gly Gln Tyr Gln Glu Pro Trp Gln Tyr Ile Asp
                1445                1450                1455    
Asp Ile Trp Leu Met Phe Asn Asn Ala Trp Leu Tyr Asn Arg Lys Thr
            1460                1465                1470        
Ser Arg Val Tyr Lys Tyr Cys Ser Lys Leu Ser Glu Val Phe Glu Gln
        1475                1480                1485            
Glu Ile Asp Pro Val Met Gln Ser Leu Gly Tyr Cys Cys Gly Arg Lys
    1490                1495                1500                
Leu Glu Phe Ser Pro Gln Thr Leu Cys Cys Tyr Gly Lys Gln Leu Cys
1505                1510                1515               1520 
Thr Ile Pro Arg Asp Ala Thr Tyr Tyr Ser Tyr Gln Asn Arg Tyr His
                1525                1530                1535    
Phe Cys Glu Lys Cys Phe Asn Glu Ile Gln Gly Glu Ser Val Ser Leu
            1540                1545                1550        
Gly Asp Asp Pro Ser Gln Pro Gln Thr Thr Ile Asn Lys Glu Gln Phe
        1555                1560                1565            
Ser Lys Arg Lys Asn Asp Thr Leu Asp Pro Glu Leu Phe Val Glu Cys
    1570                1575                1580                
Thr Glu Cys Gly Arg Lys Met His Gln Ile Cys Val Leu His His Glu
1585                1590                1595               1600 
Ile Ile Trp Pro Ser Gly Phe Val Cys Asp Gly Cys Leu Lys Lys Thr
                1605                1610                1615    
Ala Arg Thr Arg Lys Glu Asn Lys Leu Ser Ala Lys Arg Leu Pro Ser
            1620                1625                1630        
Thr Arg Leu Gly Thr Phe Leu Glu Asn Arg Val Asn Asp Phe Leu Arg
        1635                1640                1645            
Arg Gln Asn His Pro Glu Ser Gly Glu Val Thr Val Arg Val Val His
    1650                1655                1660                
Ala Ser Asp Lys Thr Val Glu Val Lys Pro Gly Met Lys Ala Arg Phe
1665                1670                1675               1680 
Val Asp Ser Gly Glu Met Ala Glu Ser Phe Pro Tyr Arg Thr Lys Ala
                1685                1690                1695    
Leu Phe Ala Phe Glu Glu Ile Asp Gly Val Asp Leu Cys Phe Phe Gly
            1700                1705                1710        
Met His Val Gln Glu Tyr Gly Ser Asp Cys Pro Pro Pro Asn Gln Arg
        1715                1720                1725            
Arg Val Tyr Ile Ser Tyr Leu Asp Ser Val His Phe Phe Arg Pro Lys
    1730                1735                1740                
Cys Leu Arg Thr Ala Val Tyr His Glu Ile Leu Ile Gly Tyr Leu Glu
1745                1750                1755               1760 
Tyr Val Lys Lys Leu Gly Tyr Thr Thr Gly His Ile Trp Ala Cys Pro
                1765                1770                1775    
Pro Ser Glu Gly Asp Asp Tyr Ile Phe His Cys His Pro Pro Asp Gln
            1780                1785                1790        
Lys Ile Pro Lys Pro Lys Arg Leu Gln Glu Trp Tyr Lys Lys Met Leu
        1795                1800                1805            
Asp Lys Ala Val Ser Glu Arg Ile Val His Asp Tyr Lys Asp Ile Leu
    1810                1815                1820                
Lys Gln Ala Thr Glu Asp Arg Leu Thr Ser Ala Lys Glu Leu Pro Tyr
1825                1830                1835               1840 
Phe Glu Gly Asp Phe Trp Pro Asn Val Leu Glu Glu Ser Ile Lys Glu
                1845                1850                1855    
Leu Glu Gln Glu Glu Glu Glu Arg Lys Arg Glu Glu Asn Thr Ser Asn
            1860                1865                1870        
Glu Ser Thr Asp Val Thr Lys Gly Asp Ser Lys Asn Ala Lys Lys Lys
        1875                1880                1885            
Asn Asn Lys Lys Thr Ser Lys Asn Lys Ser Ser Leu Ser Arg Gly Asn
    1890                1895                1900                
Lys Lys Lys Pro Gly Met Pro Asn Val Ser Asn Asp Leu Ser Gln Lys
1905                1910                1915               1920 
Leu Tyr Ala Thr Met Glu Lys His Lys Glu Val Phe Phe Val Ile Arg
                1925                1930                1935    
Leu Ile Ala Cys Pro Ala Pro Asn Ser Leu Pro Pro Ile Val Asp Pro
            1940                1945                1950        
Asp Pro Leu Ile Pro Cys Asp Leu Met Asp Gly Arg Asp Ala Phe Leu
        1955                1960                1965            
Thr Leu Ala Arg Asp Lys His Leu Glu Phe Ser Ser Leu Arg Arg Ala
    1970                1975                1980                
Gln Trp Ser Thr Met Cys Met Leu Val Glu Leu His Thr Gln Ser Gln
1985                1990                1995               2000 
Asp Arg Phe Val Tyr Thr Cys Asn Glu Cys Lys His His Val Glu Thr
                2005                2010                2015    
Arg Trp His Cys Thr Val Cys Glu Asp Tyr Asp Leu Cys Ile Thr Cys
            2020                2025                2030        
Tyr Asn Thr Lys Asn His Asp His Lys Met Glu Lys
        2035                2040                


<210> 8
<211> 2075
<212> PRT
<213> Unknown

<220> 
<223> dCas9mTet1CD

<400> 8
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Glu Ala Ala Pro Cys Asp
        1395                1400                1405            
Cys Asp Gly Gly Thr Gln Lys Glu Lys Gly Pro Tyr Tyr Thr His Leu
    1410                1415                1420                
Gly Ala Gly Pro Ser Val Ala Ala Val Arg Glu Leu Met Glu Thr Arg
1425                1430                1435               1440 
Phe Gly Gln Lys Gly Lys Ala Ile Arg Ile Glu Lys Ile Val Phe Thr
                1445                1450                1455    
Gly Lys Glu Gly Lys Ser Ser Gln Gly Cys Pro Val Ala Lys Trp Val
            1460                1465                1470        
Ile Arg Arg Ser Gly Pro Glu Glu Lys Leu Ile Cys Leu Val Arg Glu
        1475                1480                1485            
Arg Val Asp His His Cys Ser Thr Ala Val Ile Val Val Leu Ile Leu
    1490                1495                1500                
Leu Trp Glu Gly Ile Pro Arg Leu Met Ala Asp Arg Leu Tyr Lys Glu
1505                1510                1515               1520 
Leu Thr Glu Asn Leu Arg Ser Tyr Ser Gly His Pro Thr Asp Arg Arg
                1525                1530                1535    
Cys Thr Leu Asn Lys Lys Arg Thr Cys Thr Cys Gln Gly Ile Asp Pro
            1540                1545                1550        
Lys Thr Cys Gly Ala Ser Phe Ser Phe Gly Cys Ser Trp Ser Met Tyr
        1555                1560                1565            
Phe Asn Gly Cys Lys Phe Gly Arg Ser Glu Asn Pro Arg Lys Phe Arg
    1570                1575                1580                
Leu Ala Pro Asn Tyr Pro Leu His Asn Tyr Tyr Lys Arg Ile Thr Gly
1585                1590                1595               1600 
Met Ser Ser Glu Gly Ser Asp Val Lys Thr Gly Trp Ile Ile Pro Asp
                1605                1610                1615    
Arg Lys Thr Leu Ile Ser Arg Glu Glu Lys Gln Leu Glu Lys Asn Leu
            1620                1625                1630        
Gln Glu Leu Ala Thr Val Leu Ala Pro Leu Tyr Lys Gln Met Ala Pro
        1635                1640                1645            
Val Ala Tyr Gln Asn Gln Val Glu Tyr Glu Glu Val Ala Gly Asp Cys
    1650                1655                1660                
Arg Leu Gly Asn Glu Glu Gly Arg Pro Phe Ser Gly Val Thr Cys Cys
1665                1670                1675               1680 
Met Asp Phe Cys Ala His Ser His Lys Asp Ile His Asn Met His Asn
                1685                1690                1695    
Gly Ser Thr Val Val Cys Thr Leu Ile Arg Ala Asp Gly Arg Asp Thr
            1700                1705                1710        
Asn Cys Pro Glu Asp Glu Gln Leu His Val Leu Pro Leu Tyr Arg Leu
        1715                1720                1725            
Ala Asp Thr Asp Glu Phe Gly Ser Val Glu Gly Met Lys Ala Lys Ile
    1730                1735                1740                
Lys Ser Gly Ala Ile Gln Val Asn Gly Pro Thr Arg Lys Arg Arg Leu
1745                1750                1755               1760 
Arg Phe Thr Glu Pro Val Pro Arg Cys Gly Lys Arg Ala Lys Met Lys
                1765                1770                1775    
Gln Asn His Asn Lys Ser Gly Ser His Asn Thr Lys Ser Phe Ser Ser
            1780                1785                1790        
Ala Ser Ser Thr Ser His Leu Val Lys Asp Glu Ser Thr Asp Phe Cys
        1795                1800                1805            
Pro Leu Gln Ala Ser Ser Ala Glu Thr Ser Thr Cys Thr Tyr Ser Lys
    1810                1815                1820                
Thr Ala Ser Gly Gly Phe Ala Glu Thr Ser Ser Ile Leu His Cys Thr
1825                1830                1835               1840 
Met Pro Ser Gly Ala His Ser Gly Ala Asn Ala Ala Ala Gly Glu Cys
                1845                1850                1855    
Thr Gly Thr Val Gln Pro Ala Glu Val Ala Ala His Pro His Gln Ser
            1860                1865                1870        
Leu Pro Thr Ala Asp Ser Pro Val His Ala Glu Pro Leu Thr Ser Pro
        1875                1880                1885            
Ser Glu Gln Leu Thr Ser Asn Gln Ser Asn Gln Gln Leu Pro Leu Leu
    1890                1895                1900                
Ser Asn Ser Gln Lys Leu Ala Ser Cys Gln Val Glu Asp Glu Arg His
1905                1910                1915               1920 
Pro Glu Ala Asp Glu Pro Gln His Pro Glu Asp Asp Asn Leu Pro Gln
                1925                1930                1935    
Leu Asp Glu Phe Trp Ser Asp Ser Glu Glu Ile Tyr Ala Asp Pro Ser
            1940                1945                1950        
Phe Gly Gly Val Ala Ile Ala Pro Ile His Gly Ser Val Leu Ile Glu
        1955                1960                1965            
Cys Ala Arg Lys Glu Leu His Ala Thr Thr Ser Leu Arg Ser Pro Lys
    1970                1975                1980                
Arg Gly Val Pro Phe Arg Val Ser Leu Val Phe Tyr Gln His Lys Ser
1985                1990                1995               2000 
Leu Asn Lys Pro Asn His Gly Phe Asp Ile Asn Lys Ile Lys Cys Lys
                2005                2010                2015    
Cys Lys Lys Val Thr Lys Lys Lys Pro Ala Asp Arg Glu Cys Pro Asp
            2020                2025                2030        
Val Ser Pro Glu Ala Asn Leu Ser His Gln Ile Pro Ser Arg Val Ala
        2035                2040                2045            
Ser Thr Leu Thr Arg Asp Asn Val Val Thr Val Ser Pro Tyr Ser Leu
    2050                2055                2060                
Thr His Val Ala Gly Pro Tyr Asn Arg Trp Val
2065                2070                2075


<210> 9
<211> 1600
<212> PRT
<213> Unknown

<220> 
<223> dCas9hACIDA

<400> 9
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Asp Ser Leu Leu Met
        1395                1400                1405            
Asn Arg Arg Lys Phe Leu Tyr Gln Phe Lys Asn Val Arg Trp Ala Lys
    1410                1415                1420                
Gly Arg Arg Glu Thr Tyr Leu Cys Tyr Val Val Lys Arg Arg Asp Ser
1425                1430                1435               1440 
Ala Thr Ser Phe Ser Leu Asp Phe Gly Tyr Leu Arg Asn Lys Asn Gly
                1445                1450                1455    
Cys His Val Glu Leu Leu Phe Leu Arg Tyr Ile Ser Asp Trp Asp Leu
            1460                1465                1470        
Asp Pro Gly Arg Cys Tyr Arg Val Thr Trp Phe Thr Ser Trp Ser Pro
        1475                1480                1485            
Cys Tyr Asp Cys Ala Arg His Val Ala Asp Phe Leu Arg Gly Asn Pro
    1490                1495                1500                
Asn Leu Ser Leu Arg Ile Phe Thr Ala Arg Leu Tyr Phe Cys Glu Asp
1505                1510                1515               1520 
Arg Lys Ala Glu Pro Glu Gly Leu Arg Arg Leu His Arg Ala Gly Val
                1525                1530                1535    
Gln Ile Ala Ile Met Thr Phe Lys Asp Tyr Phe Tyr Cys Trp Asn Thr
            1540                1545                1550        
Phe Val Glu Asn His Glu Arg Thr Phe Lys Ala Trp Glu Gly Leu His
        1555                1560                1565            
Glu Asn Ser Val Arg Leu Ser Arg Gln Leu Arg Arg Ile Leu Leu Pro
    1570                1575                1580                
Leu Tyr Glu Val Asp Asp Leu Arg Asp Ala Phe Arg Thr Leu Gly Leu
1585                1590                1595               1600 


<210> 10
<211> 3036
<212> PRT
<213> Unknown

<220> 
<223> dCas9hDMNT1

<400> 10
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Pro Ala Arg Thr Ala
        1395                1400                1405            
Pro Ala Arg Val Pro Thr Leu Ala Val Pro Ala Ile Ser Leu Pro Asp
    1410                1415                1420                
Asp Val Arg Arg Arg Leu Lys Asp Leu Glu Arg Asp Ser Leu Thr Glu
1425                1430                1435               1440 
Lys Glu Cys Val Lys Glu Lys Leu Asn Leu Leu His Glu Phe Leu Gln
                1445                1450                1455    
Thr Glu Ile Lys Asn Gln Leu Cys Asp Leu Glu Thr Lys Leu Arg Lys
            1460                1465                1470        
Glu Glu Leu Ser Glu Glu Gly Tyr Leu Ala Lys Val Lys Ser Leu Leu
        1475                1480                1485            
Asn Lys Asp Leu Ser Leu Glu Asn Gly Ala His Ala Tyr Asn Arg Glu
    1490                1495                1500                
Val Asn Gly Arg Leu Glu Asn Gly Asn Gln Ala Arg Ser Glu Ala Arg
1505                1510                1515               1520 
Arg Val Gly Met Ala Asp Ala Asn Ser Pro Pro Lys Pro Leu Ser Lys
                1525                1530                1535    
Pro Arg Thr Pro Arg Arg Ser Lys Ser Asp Gly Glu Ala Lys Arg Ser
            1540                1545                1550        
Arg Asp Pro Pro Ala Ser Ala Ser Gln Val Thr Gly Ile Arg Ala Glu
        1555                1560                1565            
Pro Ser Pro Ser Pro Arg Ile Thr Arg Lys Ser Thr Arg Gln Thr Thr
    1570                1575                1580                
Ile Thr Ser His Phe Ala Lys Gly Pro Ala Lys Arg Lys Pro Gln Glu
1585                1590                1595               1600 
Glu Ser Glu Arg Ala Lys Ser Asp Glu Ser Ile Lys Glu Glu Asp Lys
                1605                1610                1615    
Asp Gln Asp Glu Lys Arg Arg Arg Val Thr Ser Arg Glu Arg Val Ala
            1620                1625                1630        
Arg Pro Leu Pro Ala Glu Glu Pro Glu Arg Ala Lys Ser Gly Thr Arg
        1635                1640                1645            
Thr Glu Lys Glu Glu Glu Arg Asp Glu Lys Glu Glu Lys Arg Leu Arg
    1650                1655                1660                
Ser Gln Thr Lys Glu Pro Thr Pro Lys Gln Lys Leu Lys Glu Glu Pro
1665                1670                1675               1680 
Asp Arg Glu Ala Arg Ala Gly Val Gln Ala Asp Glu Asp Glu Asp Gly
                1685                1690                1695    
Asp Glu Lys Asp Glu Lys Lys His Arg Ser Gln Pro Lys Asp Leu Ala
            1700                1705                1710        
Ala Lys Arg Arg Pro Glu Glu Lys Glu Pro Glu Lys Val Asn Pro Gln
        1715                1720                1725            
Ile Ser Asp Glu Lys Asp Glu Asp Glu Lys Glu Glu Lys Arg Arg Lys
    1730                1735                1740                
Thr Thr Pro Lys Glu Pro Thr Glu Lys Lys Met Ala Arg Ala Lys Thr
1745                1750                1755               1760 
Val Met Asn Ser Lys Thr His Pro Pro Lys Cys Ile Gln Cys Gly Gln
                1765                1770                1775    
Tyr Leu Asp Asp Pro Asp Leu Lys Tyr Gly Gln His Pro Pro Asp Ala
            1780                1785                1790        
Val Asp Glu Pro Gln Met Leu Thr Asn Glu Lys Leu Ser Ile Phe Asp
        1795                1800                1805            
Ala Asn Glu Ser Gly Phe Glu Ser Tyr Glu Ala Leu Pro Gln His Lys
    1810                1815                1820                
Leu Thr Cys Phe Ser Val Tyr Cys Lys His Gly His Leu Cys Pro Ile
1825                1830                1835               1840 
Asp Thr Gly Leu Ile Glu Lys Asn Ile Glu Leu Phe Phe Ser Gly Ser
                1845                1850                1855    
Ala Lys Pro Ile Tyr Asp Asp Asp Pro Ser Leu Glu Gly Gly Val Asn
            1860                1865                1870        
Gly Lys Asn Leu Gly Pro Ile Asn Glu Trp Trp Ile Thr Gly Phe Asp
        1875                1880                1885            
Gly Gly Glu Lys Ala Leu Ile Gly Phe Ser Thr Ser Phe Ala Glu Tyr
    1890                1895                1900                
Ile Leu Met Asp Pro Ser Pro Glu Tyr Ala Pro Ile Phe Gly Leu Met
1905                1910                1915               1920 
Gln Glu Lys Ile Tyr Ile Ser Lys Ile Val Val Glu Phe Leu Gln Ser
                1925                1930                1935    
Asn Ser Asp Ser Thr Tyr Glu Asp Leu Ile Asn Lys Ile Glu Thr Thr
            1940                1945                1950        
Val Pro Pro Ser Gly Leu Asn Leu Asn Arg Phe Thr Glu Asp Ser Leu
        1955                1960                1965            
Leu Arg His Ala Gln Phe Val Val Glu Gln Val Glu Ser Tyr Asp Glu
    1970                1975                1980                
Ala Gly Asp Ser Asp Glu Gln Pro Ile Phe Leu Thr Pro Cys Met Arg
1985                1990                1995               2000 
Asp Leu Ile Lys Leu Ala Gly Val Thr Leu Gly Gln Arg Arg Ala Gln
                2005                2010                2015    
Ala Arg Arg Gln Thr Ile Arg His Ser Thr Arg Glu Lys Asp Arg Gly
            2020                2025                2030        
Pro Thr Lys Ala Thr Thr Thr Lys Leu Val Tyr Gln Ile Phe Asp Thr
        2035                2040                2045            
Phe Phe Ala Glu Gln Ile Glu Lys Asp Asp Arg Glu Asp Lys Glu Asn
    2050                2055                2060                
Ala Phe Lys Arg Arg Arg Cys Gly Val Cys Glu Val Cys Gln Gln Pro
2065                2070                2075               2080 
Glu Cys Gly Lys Cys Lys Ala Cys Lys Asp Met Val Lys Phe Gly Gly
                2085                2090                2095    
Ser Gly Arg Ser Lys Gln Ala Cys Gln Glu Arg Arg Cys Pro Asn Met
            2100                2105                2110        
Ala Met Lys Glu Ala Asp Asp Asp Glu Glu Val Asp Asp Asn Ile Pro
        2115                2120                2125            
Glu Met Pro Ser Pro Lys Lys Met His Gln Gly Lys Lys Lys Lys Gln
    2130                2135                2140                
Asn Lys Asn Arg Ile Ser Trp Val Gly Glu Ala Val Lys Thr Asp Gly
2145                2150                2155               2160 
Lys Lys Ser Tyr Tyr Lys Lys Val Cys Ile Asp Ala Glu Thr Leu Glu
                2165                2170                2175    
Val Gly Asp Cys Val Ser Val Ile Pro Asp Asp Ser Ser Lys Pro Leu
            2180                2185                2190        
Tyr Leu Ala Arg Val Thr Ala Leu Trp Glu Asp Ser Ser Asn Gly Gln
        2195                2200                2205            
Met Phe His Ala His Trp Phe Cys Ala Gly Thr Asp Thr Val Leu Gly
    2210                2215                2220                
Ala Thr Ser Asp Pro Leu Glu Leu Phe Leu Val Asp Glu Cys Glu Asp
2225                2230                2235               2240 
Met Gln Leu Ser Tyr Ile His Ser Lys Val Lys Val Ile Tyr Lys Ala
                2245                2250                2255    
Pro Ser Glu Asn Trp Ala Met Glu Gly Gly Met Asp Pro Glu Ser Leu
            2260                2265                2270        
Leu Glu Gly Asp Asp Gly Lys Thr Tyr Phe Tyr Gln Leu Trp Tyr Asp
        2275                2280                2285            
Gln Asp Tyr Ala Arg Phe Glu Ser Pro Pro Lys Thr Gln Pro Thr Glu
    2290                2295                2300                
Asp Asn Lys Phe Lys Phe Cys Val Ser Cys Ala Arg Leu Ala Glu Met
2305                2310                2315               2320 
Arg Gln Lys Glu Ile Pro Arg Val Leu Glu Gln Leu Glu Asp Leu Asp
                2325                2330                2335    
Ser Arg Val Leu Tyr Tyr Ser Ala Thr Lys Asn Gly Ile Leu Tyr Arg
            2340                2345                2350        
Val Gly Asp Gly Val Tyr Leu Pro Pro Glu Ala Phe Thr Phe Asn Ile
        2355                2360                2365            
Lys Leu Ser Ser Pro Val Lys Arg Pro Arg Lys Glu Pro Val Asp Glu
    2370                2375                2380                
Asp Leu Tyr Pro Glu His Tyr Arg Lys Tyr Ser Asp Tyr Ile Lys Gly
2385                2390                2395               2400 
Ser Asn Leu Asp Ala Pro Glu Pro Tyr Arg Ile Gly Arg Ile Lys Glu
                2405                2410                2415    
Ile Phe Cys Pro Lys Lys Ser Asn Gly Arg Pro Asn Glu Thr Asp Ile
            2420                2425                2430        
Lys Ile Arg Val Asn Lys Phe Tyr Arg Pro Glu Asn Thr His Lys Ser
        2435                2440                2445            
Thr Pro Ala Ser Tyr His Ala Asp Ile Asn Leu Leu Tyr Trp Ser Asp
    2450                2455                2460                
Glu Glu Ala Val Val Asp Phe Lys Ala Val Gln Gly Arg Cys Thr Val
2465                2470                2475               2480 
Glu Tyr Gly Glu Asp Leu Pro Glu Cys Val Gln Val Tyr Ser Met Gly
                2485                2490                2495    
Gly Pro Asn Arg Phe Tyr Phe Leu Glu Ala Tyr Asn Ala Lys Ser Lys
            2500                2505                2510        
Ser Phe Glu Asp Pro Pro Asn His Ala Arg Ser Pro Gly Asn Lys Gly
        2515                2520                2525            
Lys Gly Lys Gly Lys Gly Lys Gly Lys Pro Lys Ser Gln Ala Cys Glu
    2530                2535                2540                
Pro Ser Glu Pro Glu Ile Glu Ile Lys Leu Pro Lys Leu Arg Thr Leu
2545                2550                2555               2560 
Asp Val Phe Ser Gly Cys Gly Gly Leu Ser Glu Gly Phe His Gln Ala
                2565                2570                2575    
Gly Ile Ser Asp Thr Leu Trp Ala Ile Glu Met Trp Asp Pro Ala Ala
            2580                2585                2590        
Gln Ala Phe Arg Leu Asn Asn Pro Gly Ser Thr Val Phe Thr Glu Asp
        2595                2600                2605            
Cys Asn Ile Leu Leu Lys Leu Val Met Ala Gly Glu Thr Thr Asn Ser
    2610                2615                2620                
Arg Gly Gln Arg Leu Pro Gln Lys Gly Asp Val Glu Met Leu Cys Gly
2625                2630                2635               2640 
Gly Pro Pro Cys Gln Gly Phe Ser Gly Met Asn Arg Phe Asn Ser Arg
                2645                2650                2655    
Thr Tyr Ser Lys Phe Lys Asn Ser Leu Val Val Ser Phe Leu Ser Tyr
            2660                2665                2670        
Cys Asp Tyr Tyr Arg Pro Arg Phe Phe Leu Leu Glu Asn Val Arg Asn
        2675                2680                2685            
Phe Val Ser Phe Lys Arg Ser Met Val Leu Lys Leu Thr Leu Arg Cys
    2690                2695                2700                
Leu Val Arg Met Gly Tyr Gln Cys Thr Phe Gly Val Leu Gln Ala Gly
2705                2710                2715               2720 
Gln Tyr Gly Val Ala Gln Thr Arg Arg Arg Ala Ile Ile Leu Ala Ala
                2725                2730                2735    
Ala Pro Gly Glu Lys Leu Pro Leu Phe Pro Glu Pro Leu His Val Phe
            2740                2745                2750        
Ala Pro Arg Ala Cys Gln Leu Ser Val Val Val Asp Asp Lys Lys Phe
        2755                2760                2765            
Val Ser Asn Ile Thr Arg Leu Ser Ser Gly Pro Phe Arg Thr Ile Thr
    2770                2775                2780                
Val Arg Asp Thr Met Ser Asp Leu Pro Glu Val Arg Asn Gly Ala Ser
2785                2790                2795               2800 
Ala Leu Glu Ile Ser Tyr Asn Gly Glu Pro Gln Ser Trp Phe Gln Arg
                2805                2810                2815    
Gln Leu Arg Gly Ala Gln Tyr Gln Pro Ile Leu Arg Asp His Ile Cys
            2820                2825                2830        
Lys Asp Met Ser Ala Leu Val Ala Ala Arg Met Arg His Ile Pro Leu
        2835                2840                2845            
Ala Pro Gly Ser Asp Trp Arg Asp Leu Pro Asn Ile Glu Val Arg Leu
    2850                2855                2860                
Ser Asp Gly Thr Met Ala Arg Lys Leu Arg Tyr Thr His His Asp Arg
2865                2870                2875               2880 
Lys Asn Gly Arg Ser Ser Ser Gly Ala Leu Arg Gly Val Cys Ser Cys
                2885                2890                2895    
Val Glu Ala Gly Lys Ala Cys Asp Pro Ala Ala Arg Gln Phe Asn Thr
            2900                2905                2910        
Leu Ile Pro Trp Cys Leu Pro His Thr Gly Asn Arg His Asn His Trp
        2915                2920                2925            
Ala Gly Leu Tyr Gly Arg Leu Glu Trp Asp Gly Phe Phe Ser Thr Thr
    2930                2935                2940                
Val Thr Asn Pro Glu Pro Met Gly Lys Gln Gly Arg Val Leu His Pro
2945                2950                2955               2960 
Glu Gln His Arg Val Val Ser Val Arg Glu Cys Ala Arg Ser Gln Gly
                2965                2970                2975    
Phe Pro Asp Thr Tyr Arg Leu Phe Gly Asn Ile Leu Asp Lys His Arg
            2980                2985                2990        
Gln Val Gly Asn Ala Val Pro Pro Pro Leu Ala Lys Ala Ile Gly Leu
        2995                3000                3005            
Glu Ile Lys Leu Cys Met Leu Ala Lys Ala Arg Glu Ser Ala Ser Ala
    3010                3015                3020                
Lys Ile Lys Glu Glu Glu Ala Ala Lys Asp Ile Asp
3025                3030                3035    


<210> 11
<211> 2316
<212> PRT
<213> Unknown

<220> 
<223> dCas9hDNMT3a

<400> 11
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Pro Ala Met Pro Ser
        1395                1400                1405            
Ser Gly Pro Gly Asp Thr Ser Ser Ser Ala Ala Glu Arg Glu Glu Asp
    1410                1415                1420                
Arg Lys Asp Gly Glu Glu Gln Glu Glu Pro Arg Gly Lys Glu Glu Arg
1425                1430                1435               1440 
Gln Glu Pro Ser Thr Thr Ala Arg Lys Val Gly Arg Pro Gly Arg Lys
                1445                1450                1455    
Arg Lys His Pro Pro Val Glu Ser Gly Asp Thr Pro Lys Asp Pro Ala
            1460                1465                1470        
Val Ile Ser Lys Ser Pro Ser Met Ala Gln Asp Ser Gly Ala Ser Glu
        1475                1480                1485            
Leu Leu Pro Asn Gly Asp Leu Glu Lys Arg Ser Glu Pro Gln Pro Glu
    1490                1495                1500                
Glu Gly Ser Pro Ala Gly Gly Gln Lys Gly Gly Ala Pro Ala Glu Gly
1505                1510                1515               1520 
Glu Gly Ala Ala Glu Thr Leu Pro Glu Ala Ser Arg Ala Val Glu Asn
                1525                1530                1535    
Gly Cys Cys Thr Pro Lys Glu Gly Arg Gly Ala Pro Ala Glu Ala Gly
            1540                1545                1550        
Lys Glu Gln Lys Glu Thr Asn Ile Glu Ser Met Lys Met Glu Gly Ser
        1555                1560                1565            
Arg Gly Arg Leu Arg Gly Gly Leu Gly Trp Glu Ser Ser Leu Arg Gln
    1570                1575                1580                
Arg Pro Met Pro Arg Leu Thr Phe Gln Ala Gly Asp Pro Tyr Tyr Ile
1585                1590                1595               1600 
Ser Lys Arg Lys Arg Asp Glu Trp Leu Ala Arg Trp Lys Arg Glu Ala
                1605                1610                1615    
Glu Lys Lys Ala Lys Val Ile Ala Gly Met Asn Ala Val Glu Glu Asn
            1620                1625                1630        
Gln Gly Pro Gly Glu Ser Gln Lys Val Glu Glu Ala Ser Pro Pro Ala
        1635                1640                1645            
Val Gln Gln Pro Thr Asp Pro Ala Ser Pro Thr Val Ala Thr Thr Pro
    1650                1655                1660                
Glu Pro Val Gly Ser Asp Ala Gly Asp Lys Asn Ala Thr Lys Ala Gly
1665                1670                1675               1680 
Asp Asp Glu Pro Glu Tyr Glu Asp Gly Arg Gly Phe Gly Ile Gly Glu
                1685                1690                1695    
Leu Val Trp Gly Lys Leu Arg Gly Phe Ser Trp Trp Pro Gly Arg Ile
            1700                1705                1710        
Val Ser Trp Trp Met Thr Gly Arg Ser Arg Ala Ala Glu Gly Thr Arg
        1715                1720                1725            
Trp Val Met Trp Phe Gly Asp Gly Lys Phe Ser Val Val Cys Val Glu
    1730                1735                1740                
Lys Leu Met Pro Leu Ser Ser Phe Cys Ser Ala Phe His Gln Ala Thr
1745                1750                1755               1760 
Tyr Asn Lys Gln Pro Met Tyr Arg Lys Ala Ile Tyr Glu Val Leu Gln
                1765                1770                1775    
Val Ala Ser Ser Arg Ala Gly Lys Leu Phe Pro Val Cys His Asp Ser
            1780                1785                1790        
Asp Glu Ser Asp Thr Ala Lys Ala Val Glu Val Gln Asn Lys Pro Met
        1795                1800                1805            
Ile Glu Trp Ala Leu Gly Gly Phe Gln Pro Ser Gly Pro Lys Gly Leu
    1810                1815                1820                
Glu Pro Pro Glu Glu Glu Lys Asn Pro Tyr Lys Glu Val Tyr Thr Asp
1825                1830                1835               1840 
Met Trp Val Glu Pro Glu Ala Ala Ala Tyr Ala Pro Pro Pro Pro Ala
                1845                1850                1855    
Lys Lys Pro Arg Lys Ser Thr Ala Glu Lys Pro Lys Val Lys Glu Ile
            1860                1865                1870        
Ile Asp Glu Arg Thr Arg Glu Arg Leu Val Tyr Glu Val Arg Gln Lys
        1875                1880                1885            
Cys Arg Asn Ile Glu Asp Ile Cys Ile Ser Cys Gly Ser Leu Asn Val
    1890                1895                1900                
Thr Leu Glu His Pro Leu Phe Val Gly Gly Met Cys Gln Asn Cys Lys
1905                1910                1915               1920 
Asn Cys Phe Leu Glu Cys Ala Tyr Gln Tyr Asp Asp Asp Gly Tyr Gln
                1925                1930                1935    
Ser Tyr Cys Thr Ile Cys Cys Gly Gly Arg Glu Val Leu Met Cys Gly
            1940                1945                1950        
Asn Asn Asn Cys Cys Arg Cys Phe Cys Val Glu Cys Val Asp Leu Leu
        1955                1960                1965            
Val Gly Pro Gly Ala Ala Gln Ala Ala Ile Lys Glu Asp Pro Trp Asn
    1970                1975                1980                
Cys Tyr Met Cys Gly His Lys Gly Thr Tyr Gly Leu Leu Arg Arg Arg
1985                1990                1995               2000 
Glu Asp Trp Pro Ser Arg Leu Gln Met Phe Phe Ala Asn Asn His Asp
                2005                2010                2015    
Gln Glu Phe Asp Pro Pro Lys Val Tyr Pro Pro Val Pro Ala Glu Lys
            2020                2025                2030        
Arg Lys Pro Ile Arg Val Leu Ser Leu Phe Asp Gly Ile Ala Thr Gly
        2035                2040                2045            
Leu Leu Val Leu Lys Asp Leu Gly Ile Gln Val Asp Arg Tyr Ile Ala
    2050                2055                2060                
Ser Glu Val Cys Glu Asp Ser Ile Thr Val Gly Met Val Arg His Gln
2065                2070                2075               2080 
Gly Lys Ile Met Tyr Val Gly Asp Val Arg Ser Val Thr Gln Lys His
                2085                2090                2095    
Ile Gln Glu Trp Gly Pro Phe Asp Leu Val Ile Gly Gly Ser Pro Cys
            2100                2105                2110        
Asn Asp Leu Ser Ile Val Asn Pro Ala Arg Lys Gly Leu Tyr Glu Gly
        2115                2120                2125            
Thr Gly Arg Leu Phe Phe Glu Phe Tyr Arg Leu Leu His Asp Ala Arg
    2130                2135                2140                
Pro Lys Glu Gly Asp Asp Arg Pro Phe Phe Trp Leu Phe Glu Asn Val
2145                2150                2155               2160 
Val Ala Met Gly Val Ser Asp Lys Arg Asp Ile Ser Arg Phe Leu Glu
                2165                2170                2175    
Ser Asn Pro Val Met Ile Asp Ala Lys Glu Val Ser Ala Ala His Arg
            2180                2185                2190        
Ala Arg Tyr Phe Trp Gly Asn Leu Pro Gly Met Asn Arg Pro Leu Ala
        2195                2200                2205            
Ser Thr Val Asn Asp Lys Leu Glu Leu Gln Glu Cys Leu Glu His Gly
    2210                2215                2220                
Arg Ile Ala Lys Phe Ser Lys Val Arg Thr Ile Thr Thr Arg Ser Asn
2225                2230                2235               2240 
Ser Ile Lys Gln Gly Lys Asp Gln His Phe Pro Val Phe Met Asn Glu
                2245                2250                2255    
Lys Glu Asp Ile Leu Trp Cys Thr Glu Met Glu Arg Val Phe Gly Phe
            2260                2265                2270        
Pro Val His Tyr Thr Asp Val Ser Asn Met Ser Arg Leu Ala Arg Gln
        2275                2280                2285            
Arg Leu Leu Gly Arg Ser Trp Ser Val Pro Val Ile Arg His Leu Phe
    2290                2295                2300                
Ala Pro Leu Lys Glu Tyr Phe Ala Cys Val Ile Asp
2305                2310                2315    


<210> 12
<211> 2172
<212> PRT
<213> Unknown

<220> 
<223> dCas9hDNMT3b

<400> 12
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Lys Gly Asp Thr Arg
        1395                1400                1405            
His Leu Asn Gly Glu Glu Asp Ala Gly Gly Arg Glu Asp Ser Ile Leu
    1410                1415                1420                
Val Asn Gly Ala Cys Ser Asp Gln Ser Ser Asp Ser Pro Pro Ile Leu
1425                1430                1435               1440 
Glu Ala Ile Arg Thr Pro Glu Ile Arg Gly Arg Arg Ser Ser Ser Arg
                1445                1450                1455    
Leu Ser Lys Arg Glu Val Ser Ser Leu Leu Ser Tyr Thr Gln Asp Leu
            1460                1465                1470        
Thr Gly Asp Gly Asp Gly Glu Asp Gly Asp Gly Ser Asp Thr Pro Val
        1475                1480                1485            
Met Pro Lys Leu Phe Arg Glu Thr Arg Thr Arg Ser Glu Ser Pro Ala
    1490                1495                1500                
Val Arg Thr Arg Asn Asn Asn Ser Val Ser Ser Arg Glu Arg His Arg
1505                1510                1515               1520 
Pro Ser Pro Arg Ser Thr Arg Gly Arg Gln Gly Arg Asn His Val Asp
                1525                1530                1535    
Glu Ser Pro Val Glu Phe Pro Ala Thr Arg Ser Leu Arg Arg Arg Ala
            1540                1545                1550        
Thr Ala Ser Ala Gly Thr Pro Trp Pro Ser Pro Pro Ser Ser Tyr Leu
        1555                1560                1565            
Thr Ile Asp Leu Thr Asp Asp Thr Glu Asp Thr His Gly Thr Pro Gln
    1570                1575                1580                
Ser Ser Ser Thr Pro Tyr Ala Arg Leu Ala Gln Asp Ser Gln Gln Gly
1585                1590                1595               1600 
Gly Met Glu Ser Pro Gln Val Glu Ala Asp Ser Gly Asp Gly Asp Ser
                1605                1610                1615    
Ser Glu Tyr Gln Asp Gly Lys Glu Phe Gly Ile Gly Asp Leu Val Trp
            1620                1625                1630        
Gly Lys Ile Lys Gly Phe Ser Trp Trp Pro Ala Met Val Val Ser Trp
        1635                1640                1645            
Lys Ala Thr Ser Lys Arg Gln Ala Met Ser Gly Met Arg Trp Val Gln
    1650                1655                1660                
Trp Phe Gly Asp Gly Lys Phe Ser Glu Val Ser Ala Asp Lys Leu Val
1665                1670                1675               1680 
Ala Leu Gly Leu Phe Ser Gln His Phe Asn Leu Ala Thr Phe Asn Lys
                1685                1690                1695    
Leu Val Ser Tyr Arg Lys Ala Met Tyr His Ala Leu Glu Lys Ala Arg
            1700                1705                1710        
Val Arg Ala Gly Lys Thr Phe Pro Ser Ser Pro Gly Asp Ser Leu Glu
        1715                1720                1725            
Asp Gln Leu Lys Pro Met Leu Glu Trp Ala His Gly Gly Phe Lys Pro
    1730                1735                1740                
Thr Gly Ile Glu Gly Leu Lys Pro Asn Asn Thr Gln Pro Glu Asn Lys
1745                1750                1755               1760 
Thr Arg Arg Arg Thr Ala Asp Asp Ser Ala Thr Ser Asp Tyr Cys Pro
                1765                1770                1775    
Ala Pro Lys Arg Leu Lys Thr Asn Cys Tyr Asn Asn Gly Lys Asp Arg
            1780                1785                1790        
Gly Asp Glu Asp Gln Ser Arg Glu Gln Met Ala Ser Asp Val Ala Asn
        1795                1800                1805            
Asn Lys Ser Ser Leu Glu Asp Gly Cys Leu Ser Cys Gly Arg Lys Asn
    1810                1815                1820                
Pro Val Ser Phe His Pro Leu Phe Glu Gly Gly Leu Cys Gln Thr Cys
1825                1830                1835               1840 
Arg Asp Arg Phe Leu Glu Leu Phe Tyr Met Tyr Asp Asp Asp Gly Tyr
                1845                1850                1855    
Gln Ser Tyr Cys Thr Val Cys Cys Glu Gly Arg Glu Leu Leu Leu Cys
            1860                1865                1870        
Ser Asn Thr Ser Cys Cys Arg Cys Phe Cys Val Glu Cys Leu Glu Val
        1875                1880                1885            
Leu Val Gly Thr Gly Thr Ala Ala Glu Ala Lys Leu Gln Glu Pro Trp
    1890                1895                1900                
Ser Cys Tyr Met Cys Leu Pro Gln Arg Cys His Gly Val Leu Arg Arg
1905                1910                1915               1920 
Arg Lys Asp Trp Asn Val Arg Leu Gln Ala Phe Phe Thr Ser Asp Thr
                1925                1930                1935    
Gly Leu Glu Tyr Glu Ala Pro Lys Leu Tyr Pro Ala Ile Pro Ala Ala
            1940                1945                1950        
Arg Arg Arg Pro Ile Arg Val Leu Ser Leu Phe Asp Gly Ile Ala Thr
        1955                1960                1965            
Gly Tyr Leu Val Leu Lys Glu Leu Gly Ile Val Gly Lys Tyr Val Ala
    1970                1975                1980                
Ser Glu Val Cys Glu Glu Ser Ile Ala Val Gly Thr Val Lys His Glu
1985                1990                1995               2000 
Gly Asn Ile Lys Tyr Val Asn Asp Val Arg Asn Ile Thr Lys Lys Asn
                2005                2010                2015    
Ile Glu Glu Trp Gly Pro Phe Asp Leu Val Ile Gly Gly Ser Pro Cys
            2020                2025                2030        
Asp Leu Ser Asn Val Asn Pro Ala Arg Lys Gly Leu Tyr Glu Gly Thr
        2035                2040                2045            
Gly Arg Leu Phe Phe Glu Phe Tyr His Leu Leu Asn Tyr Ser Arg Pro
    2050                2055                2060                
Lys Glu Gly Asp Asp Arg Pro Phe Phe Trp Met Phe Glu Asn Val Val
2065                2070                2075               2080 
Ala Met Lys Val Gly Asp Lys Arg Asp Ile Ser Arg Phe Leu Glu Cys
                2085                2090                2095    
Asn Pro Val Met Ile Asp Ala Ile Lys Val Ser Ala Ala His Arg Ala
            2100                2105                2110        
Arg Tyr Phe Trp Gly Asn Leu Pro Gly Met Asn Arg Ile Phe Gly Phe
        2115                2120                2125            
Pro Val His Tyr Thr Asp Val Ser Asn Met Gly Arg Gly Ala Arg Gln
    2130                2135                2140                
Lys Leu Leu Gly Arg Ser Trp Ser Val Pro Val Ile Arg His Leu Phe
2145                2150                2155               2160 
Ala Pro Leu Lys Asp Tyr Phe Ala Cys Glu Ile Asp
                2165                2170        


<210> 13
<211> 2576
<212> PRT
<213> Unknown

<220> 
<223> dCas9hG9a

<400> 13
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr
    530                 535                 540                 
Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser
545                 550                 555                 560 
Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg
                565                 570                 575     
Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu
            580                 585                 590         
Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala
        595                 600                 605             
Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp
    610                 615                 620                 
Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu
625                 630                 635                 640 
Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys
                645                 650                 655     
Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg
            660                 665                 670         
Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly
        675                 680                 685             
Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser
    690                 695                 700                 
Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser
705                 710                 715                 720 
Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly
                725                 730                 735     
Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile
            740                 745                 750         
Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys
        755                 760                 765             
Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg
    770                 775                 780                 
Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met
785                 790                 795                 800 
Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys
                805                 810                 815     
Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu
            820                 825                 830         
Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp
        835                 840                 845             
Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser
    850                 855                 860                 
Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp
865                 870                 875                 880 
Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys
                885                 890                 895     
Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr
            900                 905                 910         
Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser
        915                 920                 925             
Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg
    930                 935                 940                 
Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr
945                 950                 955                 960 
Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr
                965                 970                 975     
Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr
            980                 985                 990         
Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu
        995                 1000                1005            
Asn Ala Val Val Gly Thr Ala Leu Lys Lys Tyr Pro Lys Leu Glu Ser
    1010                1015                1020                
Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile
1025                1030                1035               1040 
Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe
                1045                1050                1055    
Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn
            1060                1065                1070        
Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly
        1075                1080                1085            
Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val
    1090                1095                1100                
Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr
1105                1110                1115               1120 
Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys
                1125                1130                1135    
Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe
            1140                1145                1150        
Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu
        1155                1160                1165            
Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile
    1170                1175                1180                
Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu
1185                1190                1195               1200 
Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu
                1205                1210                1215    
Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu
            1220                1225                1230        
Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser
        1235                1240                1245            
Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys
    1250                1255                1260                
Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His
1265                1270                1275               1280 
Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys
                1285                1290                1295    
Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr
            1300                1305                1310        
Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile
        1315                1320                1325            
His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr
    1330                1335                1340                
Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val
1345                1350                1355               1360 
Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr
                1365                1370                1375    
Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys Lys Arg
            1380                1385                1390        
Lys Val Glu Ala Ser Gly Pro Ala Met Ala Ala Ala Ala Gly Ala Ala
        1395                1400                1405            
Ala Ala Ala Ala Ala Glu Gly Glu Ala Pro Ala Glu Met Gly Ala Leu
    1410                1415                1420                
Leu Leu Glu Lys Glu Thr Arg Gly Ala Thr Glu Arg Val His Gly Ser
1425                1430                1435               1440 
Leu Gly Asp Thr Pro Arg Ser Glu Glu Thr Leu Pro Lys Ala Thr Pro
                1445                1450                1455    
Asp Ser Leu Glu Pro Ala Gly Pro Ser Ser Pro Ala Ser Val Thr Val
            1460                1465                1470        
Thr Val Gly Asp Glu Gly Ala Asp Thr Pro Val Gly Ala Thr Pro Leu
        1475                1480                1485            
Ile Gly Asp Glu Ser Glu Asn Leu Glu Gly Asp Gly Asp Leu Arg Gly
    1490                1495                1500                
Gly Arg Ile Leu Leu Gly His Ala Thr Lys Ser Phe Pro Ser Ser Pro
1505                1510                1515               1520 
Ser Lys Gly Gly Ser Cys Pro Ser Arg Ala Lys Met Ser Met Thr Gly
                1525                1530                1535    
Ala Gly Lys Ser Pro Pro Ser Val Gln Ser Leu Ala Met Arg Leu Leu
            1540                1545                1550        
Ser Met Pro Gly Ala Gln Gly Ala Ala Ala Ala Gly Ser Glu Pro Pro
        1555                1560                1565            
Pro Ala Thr Thr Ser Pro Glu Gly Gln Pro Lys Val His Arg Ala Arg
    1570                1575                1580                
Lys Thr Met Ser Lys Pro Gly Asn Gly Gln Pro Pro Val Pro Glu Lys
1585                1590                1595               1600 
Arg Pro Pro Glu Ile Gln His Phe Arg Met Ser Asp Asp Val His Ser
                1605                1610                1615    
Leu Gly Lys Val Thr Ser Asp Leu Ala Lys Arg Arg Lys Leu Asn Ser
            1620                1625                1630        
Gly Gly Gly Leu Ser Glu Glu Leu Gly Ser Ala Arg Arg Ser Gly Glu
        1635                1640                1645            
Val Thr Leu Thr Lys Gly Asp Pro Gly Ser Leu Glu Glu Trp Glu Thr
    1650                1655                1660                
Val Val Gly Asp Asp Phe Ser Leu Tyr Tyr Asp Ser Tyr Ser Val Asp
1665                1670                1675               1680 
Glu Arg Val Asp Ser Asp Ser Lys Ser Glu Val Glu Ala Leu Thr Glu
                1685                1690                1695    
Gln Leu Ser Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu
            1700                1705                1710        
Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Asp Glu Glu Ser Gly
        1715                1720                1725            
Asn Gln Ser Asp Arg Ser Gly Ser Ser Gly Arg Arg Lys Ala Lys Lys
    1730                1735                1740                
Lys Trp Arg Lys Asp Ser Pro Trp Val Lys Pro Ser Arg Lys Arg Arg
1745                1750                1755               1760 
Lys Arg Glu Pro Pro Arg Ala Lys Glu Pro Arg Gly Val Ser Asn Asp
                1765                1770                1775    
Thr Ser Ser Leu Glu Thr Glu Arg Gly Phe Glu Glu Leu Pro Leu Cys
            1780                1785                1790        
Ser Cys Arg Met Glu Ala Pro Lys Ile Asp Arg Ile Ser Glu Arg Ala
        1795                1800                1805            
Gly His Lys Cys Met Ala Thr Glu Ser Val Asp Gly Glu Leu Ser Gly
    1810                1815                1820                
Cys Asn Ala Ala Ile Leu Lys Arg Glu Thr Met Arg Pro Ser Ser Arg
1825                1830                1835               1840 
Val Ala Leu Met Val Leu Cys Glu Thr His Arg Ala Arg Met Val Lys
                1845                1850                1855    
His His Cys Cys Pro Gly Cys Gly Tyr Phe Cys Thr Ala Gly Thr Phe
            1860                1865                1870        
Leu Glu Cys His Pro Asp Phe Arg Val Ala His Arg Phe His Lys Ala
        1875                1880                1885            
Cys Val Ser Gln Leu Asn Gly Met Val Phe Cys Pro His Cys Gly Glu
    1890                1895                1900                
Asp Ala Ser Glu Ala Gln Glu Val Thr Ile Pro Arg Gly Asp Gly Val
1905                1910                1915               1920 
Thr Pro Pro Ala Gly Thr Ala Ala Pro Ala Pro Pro Pro Leu Ser Gln
                1925                1930                1935    
Asp Val Pro Gly Arg Ala Asp Thr Ser Gln Pro Ser Ala Arg Met Arg
            1940                1945                1950        
Gly His Gly Glu Pro Arg Arg Pro Pro Cys Asp Pro Leu Ala Asp Thr
        1955                1960                1965            
Ile Asp Ser Ser Gly Pro Ser Leu Thr Leu Pro Asn Gly Gly Cys Leu
    1970                1975                1980                
Ser Ala Val Gly Leu Pro Leu Gly Pro Gly Arg Glu Ala Leu Glu Lys
1985                1990                1995               2000 
Ala Leu Val Ile Gln Glu Ser Glu Arg Arg Lys Lys Leu Arg Phe His
                2005                2010                2015    
Pro Arg Gln Leu Tyr Leu Ser Val Lys Gln Gly Glu Leu Gln Lys Val
            2020                2025                2030        
Ile Leu Met Leu Leu Asp Asn Leu Asp Pro Asn Phe Gln Ser Asp Gln
        2035                2040                2045            
Gln Ser Lys Arg Thr Pro Leu His Ala Ala Ala Gln Lys Gly Ser Val
    2050                2055                2060                
Glu Ile Cys His Val Leu Leu Gln Ala Gly Ala Asn Ile Asn Ala Val
2065                2070                2075               2080 
Asp Lys Gln Gln Arg Thr Pro Leu Met Glu Ala Val Asn Asn His Leu
                2085                2090                2095    
Glu Val Ala Arg Tyr Met Val Gln Arg Gly Gly Cys Val Tyr Ser Lys
            2100                2105                2110        
Glu Glu Asp Gly Ser Thr Cys Leu His His Ala Ala Lys Ile Gly Asn
        2115                2120                2125            
Leu Glu Met Val Ser Leu Leu Leu Ser Thr Gly Gln Val Asp Val Asn
    2130                2135                2140                
Ala Gln Asp Ser Gly Gly Trp Thr Pro Ile Ile Trp Ala Ala Glu His
2145                2150                2155               2160 
Lys His Ile Glu Val Ile Arg Met Leu Leu Thr Arg Gly Ala Asp Val
                2165                2170                2175    
Thr Leu Thr Asp Asn Glu Glu Asn Ile Cys Leu His Trp Ala Ser Phe
            2180                2185                2190        
Thr Gly Ser Ala Ala Ile Ala Glu Val Leu Leu Asn Ala Arg Cys Asp
        2195                2200                2205            
Leu His Ala Val Asn Tyr His Gly Asp Thr Pro Leu His Ile Ala Ala
    2210                2215                2220                
Arg Glu Ser Tyr His Asp Cys Val Leu Leu Phe Leu Ser Arg Gly Ala
2225                2230                2235               2240 
Asn Pro Glu Leu Arg Asn Lys Glu Gly Asp Thr Ala Trp Asp Leu Thr
                2245                2250                2255    
Pro Glu Arg Ser Asp Val Trp Phe Ala Leu Gln Leu Asn Arg Lys Leu
            2260                2265                2270        
Arg Leu Gly Val Gly Asn Arg Ala Ile Arg Thr Glu Lys Ile Ile Cys
        2275                2280                2285            
Arg Asp Val Ala Arg Gly Tyr Glu Asn Val Pro Ile Pro Cys Val Asn
    2290                2295                2300                
Gly Val Asp Gly Glu Pro Cys Pro Glu Asp Tyr Lys Tyr Ile Ser Glu
2305                2310                2315               2320 
Asn Cys Glu Thr Ser Thr Met Asn Ile Asp Arg Asn Ile Thr His Leu
                2325                2330                2335    
Gln His Cys Thr Cys Val Asp Asp Cys Ser Ser Ser Asn Cys Leu Cys
            2340                2345                2350        
Gly Gln Leu Ser Ile Arg Cys Trp Tyr Asp Lys Asp Gly Arg Leu Leu
        2355                2360                2365            
Gln Glu Phe Asn Lys Ile Glu Pro Pro Leu Ile Phe Glu Cys Asn Gln
    2370                2375                2380                
Ala Cys Ser Cys Trp Arg Asn Cys Lys Asn Arg Val Val Gln Ser Gly
2385                2390                2395               2400 
Ile Lys Val Arg Leu Gln Leu Tyr Arg Thr Ala Lys Met Gly Trp Gly
                2405                2410                2415    
Val Arg Ala Leu Gln Thr Ile Pro Gln Gly Thr Phe Ile Cys Glu Tyr
            2420                2425                2430        
Val Gly Glu Leu Ile Ser Asp Ala Glu Ala Asp Val Arg Glu Asp Asp
        2435                2440                2445            
Ser Tyr Leu Phe Asp Leu Asp Asn Lys Asp Gly Glu Val Tyr Cys Ile
    2450                2455                2460                
Asp Ala Arg Tyr Tyr Gly Asn Ile Ser Arg Phe Ile Asn His Leu Cys
2465                2470                2475               2480 
Asp Pro Asn Ile Ile Pro Val Arg Val Phe Met Leu His Gln Asp Leu
                2485                2490                2495    
Arg Phe Pro Arg Ile Ala Phe Phe Ser Ser Arg Asp Ile Arg Thr Gly
            2500                2505                2510        
Glu Glu Leu Gly Phe Asp Tyr Gly Asp Arg Phe Trp Asp Ile Lys Ser
        2515                2520                2525            
Lys Tyr Phe Thr Cys Gln Cys Gly Ser Glu Lys Cys Lys His Ser Ala
    2530                2535                2540                
Glu Ala Ile Ala Leu Glu Gln Ser Arg Leu Ala Arg Leu Asp Pro His
2545                2550                2555               2560 
Pro Glu Leu Leu Pro Glu Leu Gly Ser Leu Pro Pro Val Asn Thr Asp
                2565                2570                2575    


<210> 14
<211> 1458
<212> PRT
<213> Unknown

<220> 
<223> dCas9VP64

<400> 14
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr
    530                 535                 540                 
Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser
545                 550                 555                 560 
Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg
                565                 570                 575     
Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu
            580                 585                 590         
Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala
        595                 600                 605             
Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp
    610                 615                 620                 
Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu
625                 630                 635                 640 
Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys
                645                 650                 655     
Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg
            660                 665                 670         
Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly
        675                 680                 685             
Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser
    690                 695                 700                 
Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser
705                 710                 715                 720 
Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly
                725                 730                 735     
Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile
            740                 745                 750         
Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys
        755                 760                 765             
Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg
    770                 775                 780                 
Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met
785                 790                 795                 800 
Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys
                805                 810                 815     
Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu
            820                 825                 830         
Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp
        835                 840                 845             
Ile Asn Arg Leu Ser Asp Tyr Asp Val Ala Ile Val Pro Gln Ser Phe
    850                 855                 860                 
Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys
865                 870                 875                 880 
Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys
                885                 890                 895     
Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln
            900                 905                 910         
Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu
        915                 920                 925             
Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln
    930                 935                 940                 
Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys
945                 950                 955                 960 
Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu
                965                 970                 975     
Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys
            980                 985                 990         
Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn
        995                 1000                1005            
Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser
    1010                1015                1020                
Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile
1025                1030                1035               1040 
Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe
                1045                1050                1055    
Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn
            1060                1065                1070        
Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly
        1075                1080                1085            
Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val
    1090                1095                1100                
Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr
1105                1110                1115               1120 
Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys
                1125                1130                1135    
Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe
            1140                1145                1150        
Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu
        1155                1160                1165            
Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile
    1170                1175                1180                
Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu
1185                1190                1195               1200 
Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu
                1205                1210                1215    
Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu
            1220                1225                1230        
Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser
        1235                1240                1245            
Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys
    1250                1255                1260                
Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His
1265                1270                1275               1280 
Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys
                1285                1290                1295    
Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr
            1300                1305                1310        
Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile
        1315                1320                1325            
His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr
    1330                1335                1340                
Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val
1345                1350                1355               1360 
Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr
                1365                1370                1375    
Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys Lys Arg
            1380                1385                1390        
Lys Val Glu Ala Ser Gly Pro Ala Gly Ser Gly Arg Ala Asp Ala Leu
        1395                1400                1405            
Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe
    1410                1415                1420                
Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp
1425                1430                1435               1440 
Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Tyr
                1445                1450                1455    
Ile Asp
        


<210> 15
<211> 1486
<212> PRT
<213> Unknown

<220> 
<223> dCas9VP96

<400> 15
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Gly Ser Gly Arg Ala Asp
        1395                1400                1405            
Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp
    1410                1415                1420                
Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp
1425                1430                1435               1440 
Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met
                1445                1450                1455    
Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser
            1460                1465                1470        
Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Tyr Ile Asp
        1475                1480                1485    


<210> 16
<211> 1537
<212> PRT
<213> Unknown

<220> 
<223> dCas9VP160

<400> 16
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Gly Ser Gly Arg Ala Asp
        1395                1400                1405            
Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp
    1410                1415                1420                
Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp
1425                1430                1435               1440 
Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met
                1445                1450                1455    
Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser
            1460                1465                1470        
Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu
        1475                1480                1485            
Asp Asp Phe Asp Leu Asp Met Leu Gly Asp Ala Leu Asp Asp Phe Asp
    1490                1495                1500                
Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met
1505                1510                1515               1520 
Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Tyr Ile
                1525                1530                1535    
Asp
    


<210> 17
<211> 1787
<212> PRT
<213> Unknown

<220> 
<223> dCas9hINI1

<400> 17
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Met Met Met Ala Leu
        1395                1400                1405            
Ser Lys Thr Phe Gly Gln Lys Pro Val Lys Phe Gln Leu Glu Asp Asp
    1410                1415                1420                
Gly Glu Phe Tyr Met Ile Gly Ser Glu Val Gly Asn Tyr Leu Arg Met
1425                1430                1435               1440 
Phe Arg Gly Ser Leu Tyr Lys Arg Tyr Pro Ser Leu Trp Arg Arg Leu
                1445                1450                1455    
Ala Thr Val Glu Glu Arg Lys Lys Ile Val Ala Ser Ser His Gly Lys
            1460                1465                1470        
Lys Thr Lys Pro Asn Thr Lys Asp His Gly Tyr Thr Thr Leu Ala Thr
        1475                1480                1485            
Ser Val Thr Leu Leu Lys Ala Ser Glu Val Glu Glu Ile Leu Asp Gly
    1490                1495                1500                
Asn Asp Glu Lys Tyr Lys Ala Val Ser Ile Ser Thr Glu Pro Pro Thr
1505                1510                1515               1520 
Tyr Leu Arg Glu Gln Lys Ala Lys Arg Asn Ser Gln Trp Val Pro Thr
                1525                1530                1535    
Leu Pro Asn Ser Ser His His Leu Asp Ala Val Pro Cys Ser Thr Thr
            1540                1545                1550        
Ile Asn Arg Asn Arg Met Gly Arg Asp Lys Lys Arg Thr Phe Pro Leu
        1555                1560                1565            
Cys Phe Asp Asp His Asp Pro Ala Val Ile His Glu Asn Ala Ser Gln
    1570                1575                1580                
Pro Glu Val Leu Val Pro Ile Arg Leu Asp Met Glu Ile Asp Gly Gln
1585                1590                1595               1600 
Lys Leu Arg Asp Ala Phe Thr Trp Asn Met Asn Glu Lys Leu Met Thr
                1605                1610                1615    
Pro Glu Met Phe Ser Glu Ile Leu Cys Asp Asp Leu Asp Leu Asn Pro
            1620                1625                1630        
Leu Thr Phe Val Pro Ala Ile Ala Ser Ala Ile Arg Gln Gln Ile Glu
        1635                1640                1645            
Ser Tyr Pro Thr Asp Ser Ile Leu Glu Asp Gln Ser Asp Gln Arg Val
    1650                1655                1660                
Ile Ile Lys Leu Asn Ile His Val Gly Asn Ile Ser Leu Val Asp Gln
1665                1670                1675               1680 
Phe Glu Trp Asp Met Ser Glu Lys Glu Asn Ser Pro Glu Lys Phe Ala
                1685                1690                1695    
Leu Lys Leu Cys Ser Glu Leu Gly Leu Gly Gly Glu Phe Val Thr Thr
            1700                1705                1710        
Ile Ala Tyr Ser Ile Arg Gly Gln Leu Ser Trp His Gln Lys Thr Tyr
        1715                1720                1725            
Ala Phe Ser Glu Asn Pro Leu Pro Thr Val Glu Ile Ala Ile Arg Asn
    1730                1735                1740                
Thr Gly Asp Ala Asp Gln Trp Cys Pro Leu Leu Glu Thr Leu Thr Asp
1745                1750                1755               1760 
Ala Glu Met Glu Lys Lys Ile Arg Asp Gln Asp Arg Asn Thr Arg Arg
                1765                1770                1775    
Met Arg Arg Leu Ala Asn Thr Ala Pro Ala Trp
            1780                1785        


<210> 18
<211> 1982
<212> PRT
<213> Unknown

<220> 
<223> dCas9hMBD4

<400> 18
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Gly Thr Thr Gly Leu
        1395                1400                1405            
Glu Ser Leu Ser Leu Gly Asp Arg Gly Ala Ala Pro Thr Val Thr Ser
    1410                1415                1420                
Ser Glu Arg Leu Val Pro Asp Pro Pro Asn Asp Leu Arg Lys Glu Asp
1425                1430                1435               1440 
Val Ala Met Glu Leu Glu Arg Val Gly Glu Asp Glu Glu Gln Met Met
                1445                1450                1455    
Ile Lys Arg Ser Ser Glu Cys Asn Pro Leu Leu Gln Glu Pro Ile Ala
            1460                1465                1470        
Ser Ala Gln Phe Gly Ala Thr Ala Gly Thr Glu Cys Arg Lys Ser Val
        1475                1480                1485            
Pro Cys Gly Trp Glu Arg Val Val Lys Gln Arg Leu Phe Gly Lys Thr
    1490                1495                1500                
Ala Gly Arg Phe Asp Val Tyr Phe Ile Ser Pro Gln Gly Leu Lys Phe
1505                1510                1515               1520 
Arg Ser Lys Ser Ser Leu Ala Asn Tyr Leu His Lys Asn Gly Glu Thr
                1525                1530                1535    
Ser Leu Lys Pro Glu Asp Phe Asp Phe Thr Val Leu Ser Lys Arg Gly
            1540                1545                1550        
Ile Lys Ser Arg Tyr Lys Asp Cys Ser Met Ala Ala Leu Thr Ser His
        1555                1560                1565            
Leu Gln Asn Gln Ser Asn Asn Ser Asn Trp Asn Leu Arg Thr Arg Ser
    1570                1575                1580                
Lys Cys Lys Lys Asp Val Phe Met Pro Pro Ser Ser Ser Ser Glu Leu
1585                1590                1595               1600 
Gln Glu Ser Arg Gly Leu Ser Asn Phe Thr Ser Thr His Leu Leu Leu
                1605                1610                1615    
Lys Glu Asp Glu Gly Val Asp Asp Val Asn Phe Arg Lys Val Arg Lys
            1620                1625                1630        
Pro Lys Gly Lys Val Thr Ile Leu Lys Gly Ile Pro Ile Lys Lys Thr
        1635                1640                1645            
Lys Lys Gly Cys Arg Lys Ser Cys Ser Gly Phe Val Gln Ser Asp Ser
    1650                1655                1660                
Lys Arg Glu Ser Val Cys Asn Lys Ala Asp Ala Glu Ser Glu Pro Val
1665                1670                1675               1680 
Ala Gln Lys Ser Gln Leu Asp Arg Thr Val Cys Ile Ser Asp Ala Gly
                1685                1690                1695    
Ala Cys Gly Glu Thr Leu Ser Val Thr Ser Glu Glu Asn Ser Leu Val
            1700                1705                1710        
Lys Lys Lys Glu Arg Ser Leu Ser Ser Gly Ser Asn Phe Cys Ser Glu
        1715                1720                1725            
Gln Lys Thr Ser Gly Ile Ile Asn Lys Phe Cys Ser Ala Lys Asp Ser
    1730                1735                1740                
Glu His Asn Glu Lys Tyr Glu Asp Thr Phe Leu Glu Ser Glu Glu Ile
1745                1750                1755               1760 
Gly Thr Lys Val Glu Val Val Glu Arg Lys Glu His Leu His Thr Asp
                1765                1770                1775    
Ile Leu Lys Arg Gly Ser Glu Met Asp Asn Asn Cys Ser Pro Thr Arg
            1780                1785                1790        
Lys Asp Phe Thr Gly Glu Lys Ile Phe Gln Glu Asp Thr Ile Pro Arg
        1795                1800                1805            
Thr Gln Ile Glu Arg Arg Lys Thr Ser Leu Tyr Phe Ser Ser Lys Tyr
    1810                1815                1820                
Asn Lys Glu Ala Leu Ser Pro Pro Arg Arg Lys Ala Phe Lys Lys Trp
1825                1830                1835               1840 
Thr Pro Pro Arg Ser Pro Phe Asn Leu Val Gln Glu Thr Leu Phe His
                1845                1850                1855    
Asp Pro Trp Lys Leu Leu Ile Ala Thr Ile Phe Leu Asn Arg Thr Ser
            1860                1865                1870        
Gly Lys Met Ala Ile Pro Val Leu Trp Lys Phe Leu Glu Lys Tyr Pro
        1875                1880                1885            
Ser Ala Glu Val Ala Arg Thr Ala Asp Trp Arg Asp Val Ser Glu Leu
    1890                1895                1900                
Leu Lys Pro Leu Gly Leu Tyr Asp Leu Arg Ala Lys Thr Ile Val Lys
1905                1910                1915               1920 
Phe Ser Asp Glu Tyr Leu Thr Lys Gln Trp Lys Tyr Pro Ile Glu Leu
                1925                1930                1935    
His Gly Ile Gly Lys Tyr Gly Asn Asp Ser Tyr Arg Ile Phe Cys Val
            1940                1945                1950        
Asn Glu Trp Lys Gln Val His Pro Glu Asp His Lys Leu Asn Lys Tyr
        1955                1960                1965            
His Asp Trp Leu Trp Glu Asn His Glu Lys Leu Ser Leu Ser
    1970                1975                1980        


<210> 19
<211> 1812
<212> PRT
<213> Unknown

<220> 
<223> dCas9hTDG

<400> 19
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Glu Ala Glu Asn Ala
        1395                1400                1405            
Gly Ser Tyr Ser Leu Gln Gln Ala Gln Ala Phe Tyr Thr Phe Pro Phe
    1410                1415                1420                
Gln Gln Leu Met Ala Glu Ala Pro Asn Met Ala Val Val Asn Glu Gln
1425                1430                1435               1440 
Gln Met Pro Glu Glu Val Pro Ala Pro Ala Pro Ala Gln Glu Pro Val
                1445                1450                1455    
Gln Glu Ala Pro Lys Gly Arg Lys Arg Lys Pro Arg Thr Thr Glu Pro
            1460                1465                1470        
Lys Gln Pro Val Glu Pro Lys Lys Pro Val Glu Ser Lys Lys Ser Gly
        1475                1480                1485            
Lys Ser Ala Lys Ser Lys Glu Lys Gln Glu Lys Ile Thr Asp Thr Phe
    1490                1495                1500                
Lys Val Lys Arg Lys Val Asp Arg Phe Asn Gly Val Ser Glu Ala Glu
1505                1510                1515               1520 
Leu Leu Thr Lys Thr Leu Pro Asp Ile Leu Thr Phe Asn Leu Asp Ile
                1525                1530                1535    
Val Ile Ile Gly Ile Asn Pro Gly Leu Met Ala Ala Tyr Lys Gly His
            1540                1545                1550        
His Tyr Pro Gly Pro Gly Asn His Phe Trp Lys Cys Leu Phe Met Ser
        1555                1560                1565            
Gly Leu Ser Glu Val Gln Leu Asn His Met Asp Asp His Thr Leu Pro
    1570                1575                1580                
Gly Lys Tyr Gly Ile Gly Phe Thr Asn Met Val Glu Arg Thr Thr Pro
1585                1590                1595               1600 
Gly Ser Lys Asp Leu Ser Ser Lys Glu Phe Arg Glu Gly Gly Arg Ile
                1605                1610                1615    
Leu Val Gln Lys Leu Gln Lys Tyr Gln Pro Arg Ile Ala Val Phe Asn
            1620                1625                1630        
Gly Lys Cys Ile Tyr Glu Ile Phe Ser Lys Glu Val Phe Gly Val Lys
        1635                1640                1645            
Val Lys Asn Leu Glu Phe Gly Leu Gln Pro His Lys Ile Pro Asp Thr
    1650                1655                1660                
Glu Thr Leu Cys Tyr Val Met Pro Ser Ser Ser Ala Arg Cys Ala Gln
1665                1670                1675               1680 
Phe Pro Arg Ala Gln Asp Lys Val His Tyr Tyr Ile Lys Leu Lys Asp
                1685                1690                1695    
Leu Arg Asp Gln Leu Lys Gly Ile Glu Arg Asn Met Asp Val Gln Glu
            1700                1705                1710        
Val Gln Tyr Thr Phe Asp Leu Gln Leu Ala Gln Glu Asp Ala Lys Lys
        1715                1720                1725            
Met Ala Val Lys Glu Glu Lys Tyr Asp Pro Gly Tyr Glu Ala Ala Tyr
    1730                1735                1740                
Gly Gly Ala Tyr Gly Glu Asn Pro Cys Ser Ser Glu Pro Cys Gly Phe
1745                1750                1755               1760 
Ser Ser Asn Gly Leu Ile Glu Ser Val Glu Leu Arg Gly Glu Ser Ala
                1765                1770                1775    
Phe Ser Gly Ile Pro Asn Gly Gln Trp Met Thr Gln Ser Phe Thr Asp
            1780                1785                1790        
Gln Ile Pro Ser Phe Ser Asn His Cys Gly Thr Gln Glu Gln Glu Glu
        1795                1800                1805            
Glu Ser His Ala
    1810        


<210> 20
<211> 2123
<212> PRT
<213> Unknown

<220> 
<223> dCas9hTET1CD

<400> 20
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Glu Leu Pro Thr Cys Ser
        1395                1400                1405            
Cys Leu Asp Arg Val Ile Gln Lys Asp Lys Gly Pro Tyr Tyr Thr His
    1410                1415                1420                
Leu Gly Ala Gly Pro Ser Val Ala Ala Val Arg Glu Ile Met Glu Asn
1425                1430                1435               1440 
Arg Tyr Gly Gln Lys Gly Asn Ala Ile Arg Ile Glu Ile Val Val Tyr
                1445                1450                1455    
Thr Gly Lys Glu Gly Lys Ser Ser His Gly Cys Pro Ile Ala Lys Trp
            1460                1465                1470        
Val Leu Arg Arg Ser Ser Asp Glu Glu Lys Val Leu Cys Leu Val Arg
        1475                1480                1485            
Gln Arg Thr Gly His His Cys Pro Thr Ala Val Met Val Val Leu Ile
    1490                1495                1500                
Met Val Trp Asp Gly Ile Pro Leu Pro Met Ala Asp Arg Leu Tyr Thr
1505                1510                1515               1520 
Glu Leu Thr Glu Asn Leu Lys Ser Tyr Asn Gly His Pro Thr Asp Arg
                1525                1530                1535    
Arg Cys Thr Leu Asn Glu Asn Arg Thr Cys Thr Cys Gln Gly Ile Asp
            1540                1545                1550        
Pro Glu Thr Cys Gly Ala Ser Phe Ser Phe Gly Cys Ser Trp Ser Met
        1555                1560                1565            
Tyr Phe Asn Gly Cys Lys Phe Gly Arg Ser Pro Ser Pro Arg Arg Phe
    1570                1575                1580                
Arg Ile Asp Pro Ser Ser Pro Leu His Glu Lys Asn Leu Glu Asp Asn
1585                1590                1595               1600 
Leu Gln Ser Leu Ala Thr Arg Leu Ala Pro Ile Tyr Lys Gln Tyr Ala
                1605                1610                1615    
Pro Val Ala Tyr Gln Asn Gln Val Glu Tyr Glu Asn Val Ala Arg Glu
            1620                1625                1630        
Cys Arg Leu Gly Ser Lys Glu Gly Arg Pro Phe Ser Gly Val Thr Ala
        1635                1640                1645            
Cys Leu Asp Phe Cys Ala His Pro His Arg Asp Ile His Asn Met Asn
    1650                1655                1660                
Asn Gly Ser Thr Val Val Cys Thr Leu Thr Arg Glu Asp Asn Arg Ser
1665                1670                1675               1680 
Leu Gly Val Ile Pro Gln Asp Glu Gln Leu His Val Leu Pro Leu Tyr
                1685                1690                1695    
Lys Leu Ser Asp Thr Asp Glu Phe Gly Ser Lys Glu Gly Met Glu Ala
            1700                1705                1710        
Lys Ile Lys Ser Gly Ala Ile Glu Val Leu Ala Pro Arg Arg Lys Lys
        1715                1720                1725            
Arg Thr Cys Phe Thr Gln Pro Val Pro Arg Ser Gly Lys Lys Arg Ala
    1730                1735                1740                
Ala Met Met Thr Glu Val Leu Ala His Lys Ile Arg Ala Val Glu Lys
1745                1750                1755               1760 
Lys Pro Ile Pro Arg Ile Lys Arg Lys Asn Asn Ser Thr Thr Thr Asn
                1765                1770                1775    
Asn Ser Lys Pro Ser Ser Leu Pro Thr Leu Gly Ser Asn Thr Glu Thr
            1780                1785                1790        
Val Gln Pro Glu Val Lys Ser Glu Thr Glu Pro His Phe Ile Leu Lys
        1795                1800                1805            
Ser Ser Asp Asn Thr Lys Thr Tyr Ser Leu Met Pro Ser Ala Pro His
    1810                1815                1820                
Pro Val Lys Glu Ala Ser Pro Gly Phe Ser Trp Ser Pro Lys Thr Ala
1825                1830                1835               1840 
Ser Ala Thr Pro Ala Pro Leu Lys Asn Asp Ala Thr Ala Ser Cys Gly
                1845                1850                1855    
Phe Ser Glu Arg Ser Ser Thr Pro His Cys Thr Met Pro Ser Gly Arg
            1860                1865                1870        
Leu Ser Gly Ala Asn Ala Ala Ala Ala Asp Gly Pro Gly Ile Ser Gln
        1875                1880                1885            
Leu Gly Glu Val Ala Pro Leu Pro Thr Leu Ser Ala Pro Val Met Glu
    1890                1895                1900                
Pro Leu Ile Asn Ser Glu Pro Ser Thr Gly Val Thr Glu Pro Leu Thr
1905                1910                1915               1920 
Pro His Gln Pro Asn His Gln Pro Ser Phe Leu Thr Ser Pro Gln Asp
                1925                1930                1935    
Leu Ala Ser Ser Pro Met Glu Glu Asp Glu Gln His Ser Glu Ala Asp
            1940                1945                1950        
Glu Pro Pro Ser Asp Glu Pro Leu Ser Asp Asp Pro Leu Ser Pro Ala
        1955                1960                1965            
Glu Glu Lys Leu Pro His Ile Asp Glu Tyr Trp Ser Asp Ser Glu His
    1970                1975                1980                
Ile Phe Leu Asp Ala Asn Ile Gly Gly Val Ala Ile Ala Pro Ala His
1985                1990                1995               2000 
Gly Ser Val Leu Ile Glu Cys Ala Arg Arg Glu Leu His Ala Thr Thr
                2005                2010                2015    
Pro Val Glu His Pro Asn Arg Asn His Pro Thr Arg Leu Ser Leu Val
            2020                2025                2030        
Phe Tyr Gln His Lys Asn Leu Asn Lys Pro Gln His Gly Phe Glu Leu
        2035                2040                2045            
Asn Lys Ile Lys Phe Glu Ala Lys Glu Ala Lys Asn Lys Lys Met Lys
    2050                2055                2060                
Ala Ser Glu Gln Lys Asp Gln Ala Ala Asn Glu Gly Pro Glu Gln Ser
2065                2070                2075               2080 
Ser Glu Val Asn Glu Leu Asn Gln Ile Pro Ser His Lys Ala Leu Thr
                2085                2090                2095    
Leu Thr His Asp Asn Val Val Thr Val Ser Pro Tyr Ala Leu Thr His
            2100                2105                2110        
Val Ala Gly Pro Tyr Asn His Trp Val Ile Asp
        2115                2120            


<210> 21
<211> 1775
<212> PRT
<213> Unknown

<220> 
<223> dCas9hPRMT1

<400> 21
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Ala Ala Ala Glu Ala
        1395                1400                1405            
Ala Asn Cys Ile Met Glu Asn Phe Val Ala Thr Leu Ala Asn Gly Met
    1410                1415                1420                
Ser Leu Gln Pro Pro Leu Glu Glu Val Ser Cys Gly Gln Ala Glu Ser
1425                1430                1435               1440 
Ser Glu Lys Pro Asn Ala Glu Asp Met Thr Ser Lys Asp Tyr Tyr Phe
                1445                1450                1455    
Asp Ser Tyr Ala His Phe Gly Ile His Glu Glu Met Leu Lys Asp Glu
            1460                1465                1470        
Val Arg Thr Leu Thr Tyr Arg Asn Ser Met Phe His Asn Arg His Leu
        1475                1480                1485            
Phe Lys Asp Lys Val Val Leu Asp Val Gly Ser Gly Thr Gly Ile Leu
    1490                1495                1500                
Cys Met Phe Ala Ala Lys Ala Gly Ala Arg Lys Val Ile Gly Ile Glu
1505                1510                1515               1520 
Cys Ser Ser Ile Ser Asp Tyr Ala Val Lys Ile Val Lys Ala Asn Lys
                1525                1530                1535    
Leu Asp His Val Val Thr Ile Ile Lys Gly Lys Val Glu Glu Val Glu
            1540                1545                1550        
Leu Pro Val Glu Lys Val Asp Ile Ile Ile Ser Glu Trp Met Gly Tyr
        1555                1560                1565            
Cys Leu Phe Tyr Glu Ser Met Leu Asn Thr Val Leu Tyr Ala Arg Asp
    1570                1575                1580                
Lys Trp Leu Ala Pro Asp Gly Leu Ile Phe Pro Asp Arg Ala Thr Leu
1585                1590                1595               1600 
Tyr Val Thr Ala Ile Glu Asp Arg Gln Tyr Lys Asp Tyr Lys Ile His
                1605                1610                1615    
Trp Trp Glu Asn Val Tyr Gly Phe Asp Met Ser Cys Ile Lys Asp Val
            1620                1625                1630        
Ala Ile Lys Glu Pro Leu Val Asp Val Val Asp Pro Lys Gln Leu Val
        1635                1640                1645            
Thr Asn Ala Cys Leu Ile Lys Glu Val Asp Ile Tyr Thr Val Lys Val
    1650                1655                1660                
Glu Asp Leu Thr Phe Thr Ser Pro Phe Cys Leu Gln Val Lys Arg Asn
1665                1670                1675               1680 
Asp Tyr Val His Ala Leu Val Ala Tyr Phe Asn Ile Glu Phe Thr Arg
                1685                1690                1695    
Cys His Lys Arg Thr Gly Phe Ser Thr Ser Pro Glu Ser Pro Tyr Thr
            1700                1705                1710        
His Trp Lys Gln Thr Val Phe Tyr Met Glu Asp Tyr Leu Thr Val Lys
        1715                1720                1725            
Thr Gly Glu Glu Ile Phe Gly Thr Ile Gly Met Arg Pro Asn Ala Lys
    1730                1735                1740                
Asn Asn Arg Asp Leu Asp Phe Thr Ile Asp Leu Asp Phe Lys Gly Gln
1745                1750                1755               1760 
Leu Cys Glu Leu Ser Cys Ser Thr Asp Tyr Arg Met Arg Ile Asp
                1765                1770                1775


<210> 22
<211> 1770
<212> PRT
<213> Unknown

<220> 
<223> dCas9hSET7

<400> 22
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Asp Ser Asp Asp Glu
        1395                1400                1405            
Met Val Glu Glu Ala Val Glu Gly His Leu Asp Asp Asp Gly Leu Pro
    1410                1415                1420                
His Gly Phe Cys Thr Val Thr Tyr Ser Ser Thr Asp Arg Phe Glu Gly
1425                1430                1435               1440 
Asn Phe Val His Gly Glu Lys Asn Gly Arg Gly Lys Phe Phe Phe Phe
                1445                1450                1455    
Asp Gly Ser Thr Leu Glu Gly Tyr Tyr Val Asp Asp Ala Leu Gln Gly
            1460                1465                1470        
Gln Gly Val Tyr Thr Tyr Glu Asp Gly Gly Val Leu Gln Gly Thr Tyr
        1475                1480                1485            
Val Asp Gly Glu Leu Asn Gly Pro Ala Gln Glu Tyr Asp Thr Asp Gly
    1490                1495                1500                
Arg Leu Ile Phe Lys Gly Gln Tyr Lys Asp Asn Ile Arg His Gly Val
1505                1510                1515               1520 
Cys Trp Ile Tyr Tyr Pro Asp Gly Gly Ser Leu Val Gly Glu Val Asn
                1525                1530                1535    
Glu Asp Gly Glu Met Thr Gly Glu Lys Ile Ala Tyr Val Tyr Pro Asp
            1540                1545                1550        
Glu Arg Thr Ala Leu Tyr Gly Lys Phe Ile Asp Gly Glu Met Ile Glu
        1555                1560                1565            
Gly Lys Leu Ala Thr Leu Met Ser Thr Glu Glu Gly Arg Pro His Phe
    1570                1575                1580                
Glu Leu Met Pro Gly Asn Ser Val Tyr His Phe Asp Lys Ser Thr Ser
1585                1590                1595               1600 
Ser Cys Ile Ser Thr Asn Ala Leu Leu Pro Asp Pro Tyr Glu Ser Glu
                1605                1610                1615    
Arg Val Tyr Val Ala Glu Ser Leu Ile Ser Ser Ala Gly Glu Gly Leu
            1620                1625                1630        
Phe Ser Lys Val Ala Val Gly Pro Asn Thr Val Met Ser Phe Tyr Asn
        1635                1640                1645            
Gly Val Arg Ile Thr His Gln Glu Val Asp Ser Arg Asp Trp Ala Leu
    1650                1655                1660                
Asn Gly Asn Thr Leu Ser Leu Asp Glu Glu Thr Val Ile Asp Val Pro
1665                1670                1675               1680 
Glu Pro Tyr Asn His Val Ser Lys Tyr Cys Ala Ser Leu Gly His Lys
                1685                1690                1695    
Ala Asn His Ser Phe Thr Pro Asn Cys Ile Tyr Asp Met Phe Val His
            1700                1705                1710        
Pro Arg Phe Gly Pro Ile Lys Cys Ile Arg Thr Leu Arg Ala Val Glu
        1715                1720                1725            
Ala Asp Glu Glu Leu Thr Val Ala Tyr Gly Tyr Asp His Ser Pro Pro
    1730                1735                1740                
Gly Lys Ser Gly Pro Glu Ala Pro Glu Trp Tyr Gln Val Glu Leu Lys
1745                1750                1755               1760 
Ala Phe Gln Ala Thr Gln Gln Lys Ile Asp
                1765                1770


<210> 23
<211> 1550
<212> PRT
<213> Unknown

<220> 
<223> dCas9SID4x

<400> 23
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Ala Ser Pro Lys Lys Lys
        1395                1400                1405            
Arg Lys Val Glu Ala Ser Gly Ser Gly Met Asn Ile Gln Met Leu Leu
    1410                1415                1420                
Glu Ala Ala Asp Tyr Leu Glu Arg Arg Glu Arg Glu Ala Glu His Gly
1425                1430                1435               1440 
Tyr Ala Ser Met Leu Pro Gly Ser Gly Met Asn Ile Gln Met Leu Leu
                1445                1450                1455    
Glu Ala Ala Asp Tyr Leu Glu Arg Arg Glu Arg Glu Ala Glu His Gly
            1460                1465                1470        
Tyr Ala Ser Met Leu Pro Gly Ser Gly Met Asn Ile Gln Met Leu Leu
        1475                1480                1485            
Glu Ala Ala Asp Tyr Leu Glu Arg Arg Glu Arg Glu Ala Glu His Gly
    1490                1495                1500                
Tyr Ala Ser Met Leu Pro Gly Ser Gly Met Asn Ile Gln Met Leu Leu
1505                1510                1515               1520 
Glu Ala Ala Asp Tyr Leu Glu Arg Arg Glu Arg Glu Ala Glu His Gly
                1525                1530                1535    
Tyr Ala Ser Met Leu Pro Ser Arg Ser Arg Ile Asp Ile Asp
            1540                1545                1550


<210> 24
<211> 1788
<212> PRT
<213> Unknown

<220> 
<223> dCas9hsSssIM

<400> 24
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Ser Lys Val Glu Asn
        1395                1400                1405            
Lys Thr Lys Lys Leu Arg Val Phe Glu Ala Phe Ala Gly Ile Gly Ala
    1410                1415                1420                
Gln Arg Lys Ala Leu Glu Lys Val Arg Lys Asp Glu Tyr Glu Ile Val
1425                1430                1435               1440 
Gly Leu Ala Glu Trp Tyr Val Pro Ala Ile Val Met Tyr Gln Ala Ile
                1445                1450                1455    
His Asn Asn Phe His Thr Lys Leu Glu Tyr Lys Ser Val Ser Arg Glu
            1460                1465                1470        
Glu Met Ile Asp Tyr Leu Glu Asn Lys Thr Leu Ser Trp Asn Ser Lys
        1475                1480                1485            
Asn Pro Val Ser Asn Gly Tyr Trp Lys Arg Lys Lys Asp Asp Glu Leu
    1490                1495                1500                
Lys Ile Ile Tyr Asn Ala Ile Lys Leu Ser Glu Lys Glu Gly Asn Ile
1505                1510                1515               1520 
Phe Asp Ile Arg Asp Leu Tyr Lys Arg Thr Leu Lys Asn Ile Asp Leu
                1525                1530                1535    
Leu Thr Tyr Ser Phe Pro Cys Gln Asp Leu Ser Gln Gln Gly Ile Gln
            1540                1545                1550        
Lys Gly Met Lys Arg Gly Ser Gly Thr Arg Ser Gly Leu Leu Trp Glu
        1555                1560                1565            
Ile Glu Arg Ala Leu Asp Ser Thr Glu Lys Asn Asp Leu Pro Lys Tyr
    1570                1575                1580                
Leu Leu Met Glu Asn Val Gly Ala Leu Leu His Lys Lys Asn Glu Glu
1585                1590                1595               1600 
Glu Leu Asn Gln Trp Lys Gln Lys Leu Glu Ser Leu Gly Tyr Gln Asn
                1605                1610                1615    
Ser Ile Glu Val Leu Asn Ala Ala Asp Phe Gly Ser Ser Gln Ala Arg
            1620                1625                1630        
Arg Arg Val Phe Met Ile Ser Thr Leu Asn Glu Phe Val Glu Leu Pro
        1635                1640                1645            
Lys Gly Asp Lys Lys Pro Lys Ser Ile Lys Lys Val Leu Asn Lys Ile
    1650                1655                1660                
Val Ser Glu Lys Asp Ile Leu Asn Asn Leu Leu Lys Tyr Asn Leu Thr
1665                1670                1675               1680 
Glu Phe Lys Lys Thr Lys Ser Asn Ile Asn Lys Ala Ser Leu Ile Gly
                1685                1690                1695    
Tyr Ser Lys Phe Asn Ser Glu Gly Tyr Val Tyr Asp Pro Glu Phe Thr
            1700                1705                1710        
Gly Pro Thr Leu Thr Ala Ser Gly Ala Asn Ser Arg Ile Lys Ile Lys
        1715                1720                1725            
Asp Gly Ser Asn Ile Arg Lys Met Asn Ser Asp Glu Thr Phe Leu Tyr
    1730                1735                1740                
Met Gly Phe Asp Ser Gln Asp Gly Lys Arg Val Asn Glu Ile Glu Phe
1745                1750                1755               1760 
Leu Thr Glu Asn Gln Lys Ile Phe Val Ala Gly Asn Ser Ile Ser Val
                1765                1770                1775    
Glu Val Leu Glu Ala Ile Ile Asp Lys Ile Gly Gly
            1780                1785            


<210> 25
<211> 1706
<212> PRT
<213> Unknown

<220> 
<223> dCas9hsSssIMA

<400> 25
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Ser Lys Val Glu Asn
        1395                1400                1405            
Lys Thr Lys Lys Leu Arg Val Phe Glu Ala Phe Ala Gly Ile Gly Ala
    1410                1415                1420                
Gln Arg Lys Ala Leu Glu Lys Val Arg Lys Asp Glu Tyr Glu Ile Val
1425                1430                1435               1440 
Gly Leu Ala Glu Trp Tyr Val Pro Ala Ile Val Met Tyr Gln Ala Ile
                1445                1450                1455    
His Asn Asn Phe His Thr Lys Leu Glu Tyr Lys Ser Val Ser Arg Glu
            1460                1465                1470        
Glu Met Ile Asp Tyr Leu Glu Asn Lys Thr Leu Ser Trp Asn Ser Lys
        1475                1480                1485            
Asn Pro Val Ser Asn Gly Tyr Trp Lys Arg Lys Lys Asp Asp Glu Leu
    1490                1495                1500                
Lys Ile Ile Tyr Asn Ala Ile Lys Leu Ser Glu Lys Glu Gly Asn Ile
1505                1510                1515               1520 
Phe Asp Ile Arg Asp Leu Tyr Lys Arg Thr Leu Lys Asn Ile Asp Leu
                1525                1530                1535    
Leu Thr Tyr Ser Phe Pro Cys Gln Asp Leu Ser Gln Gln Gly Ile Gln
            1540                1545                1550        
Lys Gly Met Lys Arg Gly Ser Gly Thr Arg Ser Gly Leu Leu Trp Glu
        1555                1560                1565            
Ile Glu Arg Ala Leu Asp Ser Thr Glu Lys Asn Asp Leu Pro Lys Tyr
    1570                1575                1580                
Leu Leu Met Glu Asn Val Gly Ala Leu Leu His Lys Lys Asn Glu Glu
1585                1590                1595               1600 
Glu Leu Asn Gln Trp Lys Gln Lys Leu Glu Ser Leu Gly Tyr Gln Asn
                1605                1610                1615    
Ser Ile Glu Val Leu Asn Ala Ala Asp Phe Gly Ser Ser Gln Ala Arg
            1620                1625                1630        
Arg Arg Val Phe Met Ile Ser Thr Leu Asn Glu Phe Val Glu Leu Pro
        1635                1640                1645            
Lys Gly Asp Lys Lys Pro Lys Ser Ile Lys Lys Val Leu Asn Lys Ile
    1650                1655                1660                
Val Ser Glu Lys Asp Ile Leu Asn Asn Leu Leu Lys Tyr Asn Leu Thr
1665                1670                1675               1680 
Glu Phe Lys Lys Thr Lys Ser Asn Ile Asn Lys Ala Ser Leu Ile Gly
                1685                1690                1695    
Tyr Ser Lys Phe Asn Ser Glu Gly Tyr Val
            1700                1705    


<210> 26
<211> 1548
<212> PRT
<213> Unknown

<220> 
<223> dCas9hsSssIMB

<400> 26
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Glu Phe Val Glu Leu Pro
        1395                1400                1405            
Lys Gly Asp Lys Lys Pro Lys Ser Ile Lys Lys Val Leu Asn Lys Ile
    1410                1415                1420                
Val Ser Glu Lys Asp Ile Leu Asn Asn Leu Leu Lys Tyr Asn Leu Thr
1425                1430                1435               1440 
Glu Phe Lys Lys Thr Lys Ser Asn Ile Asn Lys Ala Ser Leu Ile Gly
                1445                1450                1455    
Tyr Ser Lys Phe Asn Ser Glu Gly Tyr Val Tyr Asp Pro Glu Phe Thr
            1460                1465                1470        
Gly Pro Thr Leu Thr Ala Ser Gly Ala Asn Ser Arg Ile Lys Ile Lys
        1475                1480                1485            
Asp Gly Ser Asn Ile Arg Lys Met Asn Ser Asp Glu Thr Phe Leu Tyr
    1490                1495                1500                
Met Gly Phe Asp Ser Gln Asp Gly Lys Arg Val Asn Glu Ile Glu Phe
1505                1510                1515               1520 
Leu Thr Glu Asn Gln Lys Ile Phe Val Ala Gly Asn Ser Ile Ser Val
                1525                1530                1535    
Glu Val Leu Glu Ala Ile Ile Asp Lys Ile Gly Gly
            1540                1545            


<210> 27
<211> 43
<212> DNA
<213> Unknown

<220> 
<223> targeting site in Tet1

<400> 27
agtgtggctg ctgtcaggga gctcatggag actaggtgag gaa                   43

<210> 28
<211> 44
<212> DNA
<213> Unknown

<220> 
<223> targeting site in Tet2

<400> 28
cacgtgaaag tgccaacaga tatccaggct gcagaatcgg agaa                  44

<210> 29
<211> 45
<212> DNA
<213> Unknown

<220> 
<223> targeting site in Tet3

<400> 29
ggcaaggagg ggaagagttc tcgaggctgt cccatcgcca agtgg                 45

<210> 30
<211> 56
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet1 (clone #14)

<400> 30
gaccaagtgt ggctgctgtc agggagctca tggagactag gtgaggaact ctgctt     56

<210> 31
<211> 55
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet1 (clone #14)

<400> 31
gaccaagtgt ggctgctgtc agggagtcat ggagactagg tgaggaactc tgctt      55

<210> 32
<211> 57
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet1 (clone #14)

<400> 32
gaccaagtgt ggctgctgtc agggagcctc atggagacta ggtgaggaac tctgctt    57

<210> 33
<211> 56
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet2 (clone #14)

<400> 33
agaaacacgt gaaagtgcca acagatatcc aggctgcaga atcggagaac cacgcc     56

<210> 34
<211> 41
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet2 (clone #14)

<400> 34
agaaacacgt gaaagtgcca acagaatcgg agaaccacgc c                     41

<210> 35
<211> 53
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet2 (clone #14)

<400> 35
agaaacacgt gaaagtgcca acagtccagg ctgcagaatc ggagaaccac gcc        53

<210> 36
<211> 56
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet3 (clone #14)

<400> 36
tcatctacac gggcaaggag gggaagagtt ctcgaggctg tcccatcgcc aagtgg     56

<210> 37
<211> 45
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet3 (clone #14)

<400> 37
tcatctacac gggcaaggag gggaagatgt cccatcgcca agtgg                 45

<210> 38
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet3 (clone #14)

<400> 38
tcatctacac gggcaaggag                                             20

<210> 39
<211> 53
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet1 (clone #41)

<400> 39
gaccaagtgt ggctgctgtc agggagctgg agactaggtg aggaactctg ctt        53

<210> 40
<211> 47
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet1 (clone #41)

<400> 40
gaccaagtgt ggctgctgtc agggagacta ggtgaggaac tctgctt               47

<210> 41
<211> 47
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet2 (clone #41)

<400> 41
agaaacacgt gaaagtgctc caggctgcag aatcggagaa ccacgcc               47

<210> 42
<211> 57
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet2 (clone #41)

<400> 42
agaaacacgt gaaagtgcca acagatattc caggctgcag aatcggagaa ccacgcc    57

<210> 43
<211> 56
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet3 (clone #41)

<400> 43
atctacacgg gcaaggaggg gaagagttct cgaggctgtc ccatcgccaa gtgggt     56

<210> 44
<211> 32
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet3 (clone #41)

<400> 44
atctacacgg gcaaggaggg gaagagtgta gt                               32

<210> 45
<211> 48
<212> DNA
<213> Unknown

<220> 
<223> targeted site in mutant allele of Tet3 (clone #41)

<400> 45
atctacacgg gcaaggaggg gaagaggctg tcccatcgcc aagtgggt              48

<210> 46
<211> 47
<212> DNA
<213> Unknown

<220> 
<223> targeted sequence of Tet1 #2

<400> 46
gaccaagtgt ggctgctgtc atggagacta ggtgaggaac tctgctt               47

<210> 47
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> targeted sequence of Tet2 #4

<400> 47
agaaacacgt gaaagtgcca acag                                        24

<210> 48
<211> 26
<212> DNA
<213> Unknown

<220> 
<223> Tet1 mutant allele from double mutant mouse #9

<400> 48
tggagactag gtgaggaact ctgctt                                      26

<210> 49
<211> 57
<212> DNA
<213> Unknown

<220> 
<223> Tet2 mutant allele from double mutant mouse #9

<400> 49
agaaacacgt gaaagtgcca acagataatc caggctgcag aatcggagaa ccacgcc    57

<210> 50
<211> 32
<212> DNA
<213> Unknown

<220> 
<223> Tet1 mutant allele from double mutant mouse #10

<400> 50
gacccatgga gactaggtga ggaactctgc tt                               32

<210> 51
<211> 47
<212> DNA
<213> Unknown

<220> 
<223> Tet1 mutant allele from double mutant mouse #10

<400> 51
gaccaagtgt ggctgctgtc agggagacta ggtgaggaac tctgctt               47

<210> 52
<211> 48
<212> DNA
<213> Unknown

<220> 
<223> Tet2 mutant allele from double mutant mouse #10

<400> 52
agaaacacgt gaaagtgcca acaggctgca gaatcggaga accacgcc              48

<210> 53
<211> 57
<212> DNA
<213> Unknown

<220> 
<223> Tet2 mutant allele from double mutant mouse #10

<400> 53
agaaacacgt gaaagtgcca acagattctc caggctgcag aatcggagaa ccacgcc    57

<210> 54
<211> 43
<212> DNA
<213> Unknown

<220> 
<223> oligo targeting site of Tet1

<400> 54
agtgtggctg ctgtcaggga gctcatggag actaggtgag gaa                   43

<210> 55
<211> 43
<212> DNA
<213> Unknown

<220> 
<223> oligo targeting site of Tet1

<400> 55
agtgtggctg ctgtcaggga attcatggag actaggtgag gaa                   43

<210> 56
<211> 44
<212> DNA
<213> Unknown

<220> 
<223> oligo targeting site of Tet2

<400> 56
cacgtgaaag tgccaacaga tatccaggct gcagaatcgg agaa                  44

<210> 57
<211> 44
<212> DNA
<213> Unknown

<220> 
<223> oligo targeting site of Tet2

<400> 57
cacgtgaaag tgccaacaga attccaggct gcagaatcgg agaa                  44

<210> 58
<211> 56
<212> DNA
<213> Unknown

<220> 
<223> allele of Tet1 in mouse #5

<400> 58
gaccaagtgt ggctgctgtc agggaattca tggagactag gtgaggaact ctgctt     56

<210> 59
<211> 56
<212> DNA
<213> Unknown

<220> 
<223> allele of Tet2 in mouse #5

<400> 59
agaaacacgt gaaagtgcca acagaattcc aggctgcaga atcggagaac cacgcc     56

<210> 60
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> allele of Tet1 in mouse #7

<400> 60
gactaggtga ggaactctgc tt                                          22

<210> 61
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> allele of Tet2 in mouse #7

<400> 61
gctgcagaat cggagaacca cgcc                                        24

<210> 62
<211> 42
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence in Sry

<400> 62
gaatgcattt atggtgtggt cccgtggtga gaggcacaag tt                    42

<210> 63
<211> 38
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence in Uty

<400> 63
tactgctgcc ctaggtaatg aggaaaagaa aatggcgg                         38

<210> 64
<211> 38
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence in Uty

<400> 64
ccgccatttt cttttcctca ttacctaggg cagcagta                         38

<210> 65
<211> 43
<212> DNA
<213> Unknown

<220> 
<223> Tet3 allele of surviving Tet3 targeted mouse #14

<400> 65
tcatctacac gggcaaggag gggaagatcc catcgccaag tgg                   43

<210> 66
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> Tet1 targeting site

<400> 66
ggctgctgtc agggagctca tgg                                         23

<210> 67
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> Tet1 off target site 1810013L24Rik

<400> 67
ctgtttggtc agggagctca agg                                         23

<210> 68
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> Tet1 off target site Lipg

<400> 68
gggccaagtc agggagctca agg                                         23

<210> 69
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> Tet1 off target site Olfr339

<400> 69
gtttagtgtc agggagctca ggg                                         23

<210> 70
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> Tet2 targeting site

<400> 70
gaaagtgcca acagatatcc agg                                         23

<210> 71
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> Tet2 off target site Ubrf

<400> 71
gcaaagacca acagatatcc agg                                         23

<210> 72
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> Tet2 off target site AKi16506

<400> 72
aggaaaccca acagatatcc cgg                                         23

<210> 73
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> Tet2 off target site Cyp2c55

<400> 73
ccacctccca acagatatcc tgg                                         23

<210> 74
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> Tet2 off target site E430025E21Rik

<400> 74
gagataacca acagatatcc tgg                                         23

<210> 75
<211> 46
<212> DNA
<213> Unknown

<220> 
<223> Tet1 sequence from embryo #2

<400> 75
gaccaagtgt ggctgctgca tggagactag gtgaggaact ctgctt                46

<210> 76
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> 20 bp sequence of Tet1

<400> 76
ggctgctgtc agggagctca                                             20

<210> 77
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> 20 bp sequence of Tet2

<400> 77
gaaagtgcca acagatatcc                                             20

<210> 78
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> 20 bp sequence of Tet3

<400> 78
aaggagggga agagttctcg                                             20

<210> 79
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> 20 bp sequence of Sry

<400> 79
gcatttatgg tgtggtcccg                                             20

<210> 80
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> 20 bp sequence of Uty

<400> 80
tttcttttcc tcattaccta                                             20

<210> 81
<211> 102
<212> RNA
<213> Unknown

<220> 
<223> Tet1

<400> 81
ggcugcuguc agggagcuca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc 60
cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uu                    102

<210> 82
<211> 102
<212> RNA
<213> Unknown

<220> 
<223> Tet2

<400> 82
gaaagugcca acagauaucc guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc 60
cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uu                    102

<210> 83
<211> 103
<212> RNA
<213> Unknown

<220> 
<223> Tet3

<400> 83
gaaggagggg aagaguucuc gguuuuagag cuagaaauag caaguuaaaa uaaggcuagu 60
ccguuaucaa cuugaaaaag uggcaccgag ucggugcuuu uuu                   103

<210> 84
<211> 102
<212> RNA
<213> Unknown

<220> 
<223> Sry

<400> 84
gcauuuaugg uguggucccg guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc 60
cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uu                    102

<210> 85
<211> 103
<212> RNA
<213> Unknown

<220> 
<223> Uty

<400> 85
guuucuuuuc cucauuaccu aguuuuagag cuagaaauag caaguuaaaa uaaggcuagu 60
ccguuaucaa cuugaaaaag uggcaccgag ucggugcuuu uuu                   103

<210> 86
<211> 83
<212> PRT
<213> Unknown

<220> 
<223> HMG box sequence of human Sry

<400> 86
Asp Arg Val Lys Arg Pro Met Ile Ile Ala Phe Ile Val Trp Ser Arg
 1               5                  10                  15      
Asp Gln Arg Arg Lys Met Ala Leu Glu Ile Ile Pro Arg Met Arg Ile
            20                  25                  30          
Ile Ser Glu Ile Ser Lys Gln Leu Gly Tyr Gln Trp Lys Met Leu Thr
        35                  40                  45              
Glu Ala Glu Lys Trp Pro Phe Phe Gln Glu Ala Gln Lys Leu Gln Ala
    50                  55                  60                  
Met His Arg Glu Lys Tyr Pro Ile Ile Tyr Lys Tyr Arg Pro Arg Arg
65                  70                  75                  80  
Lys Ala Lys
            


<210> 87
<211> 84
<212> PRT
<213> Unknown

<220> 
<223> HMG box sequence of mouse Sry

<400> 87
Gly His Val Lys Arg Pro Met Ile Ile Ala Phe Met Val Trp Ser Arg
 1               5                  10                  15      
Gly Glu Arg His Lys Leu Ala Gln Gln Ile Ile Pro Asp Met Gln Ile
            20                  25                  30          
Ile Thr Glu Ile Ser Lys Gln Leu Gly Cys Arg Trp Lys Ser Leu Thr
        35                  40                  45              
Glu Ala Glu Lys Arg Pro Phe Phe Gln Glu Ala Gln Arg Leu Lys Ile
    50                  55                  60                  
Leu His Arg Glu Lys Tyr Pro Ile Ile Tyr Lys Tyr Gln Pro His Arg
65                  70                  75                  80  
Arg Ala Lys Val
                


<210> 88
<211> 84
<212> PRT
<213> Unknown

<220> 
<223> HMG box sequence of chimpanzee Sry

<400> 88
Asp Arg Val Lys Arg Pro Met Ile Ile Ala Phe Phe Val Trp Ser Arg
 1               5                  10                  15      
Asp Gln Arg Arg Lys Met Ala Leu Glu Ile Ile Pro Arg Met Arg Ile
            20                  25                  30          
Ile Ser Glu Ile Ser Lys Gln Leu Gly Tyr Gln Trp Lys Met Leu Thr
        35                  40                  45              
Glu Ala Glu Lys Trp Pro Phe Phe Gln Glu Ala Gln Lys Leu Gln Ala
    50                  55                  60                  
Met His Arg Glu Lys Tyr Pro Ile Ile Tyr Lys Tyr Arg Pro Arg Arg
65                  70                  75                  80  
Lys Ala Ile Ile
                


<210> 89
<211> 83
<212> PRT
<213> Unknown

<220> 
<223> HMG box sequence of Hylobates Sry

<400> 89
Asp Arg Val Lys Arg Pro Met Ile Ile Ala Phe Ile Val Trp Ser Arg
 1               5                  10                  15      
Asp Gln Arg Arg Lys Met Ala Leu Glu Ile Ile Pro Lys Met Arg Ile
            20                  25                  30          
Ile Ser Glu Ile Ser Lys Gln Leu Gly Tyr Arg Trp Lys Met Leu Thr
        35                  40                  45              
Glu Ala Glu Lys Trp Pro Phe Phe Gln Glu Ala Gln Lys Leu Gln Ala
    50                  55                  60                  
Met His Arg Glu Lys Tyr Pro Ile Ile Tyr Lys Tyr Arg Pro Arg Arg
65                  70                  75                  80  
Lys Ala Lys
            


<210> 90
<211> 83
<212> PRT
<213> Unknown

<220> 
<223> HMG box sequence of baboon Sry

<400> 90
Asp Arg Val Lys Arg Pro Met Ile Ile Ala Phe Ile Val Trp Ser Arg
 1               5                  10                  15      
Asp Gln Arg Arg Lys Met Ala Leu Glu Ile Ile Pro Lys Met Arg Ile
            20                  25                  30          
Ile Ser Glu Ile Ser Lys Gln Leu Gly Tyr Gln Trp Lys Met Leu Thr
        35                  40                  45              
Glu Ala Asp Lys Trp Pro Phe Phe Gln Glu Ala Gln Lys Leu Gln Ala
    50                  55                  60                  
Met His Arg Glu Lys Tyr Pro Ile Ile Tyr Lys Tyr Arg Pro Arg Arg
65                  70                  75                  80  
Lys Ala Lys
            


<210> 91
<211> 84
<212> PRT
<213> Unknown

<220> 
<223> HMG box sequence of Calitrix Sry

<400> 91
Ile Ile Arg Val Lys Arg Pro Met Ile Ala Phe Ile Val Trp Ser Arg
 1               5                  10                  15      
Asp Gln Arg Arg Lys Met Ala Val Glu Ile Ile Pro Asp Met Arg Ile
            20                  25                  30          
Ile Ser Glu Ile Ser Lys Arg Leu Gly Tyr Gln Trp Lys Leu Leu Thr
        35                  40                  45              
Glu Ala Glu Lys Trp Pro Phe Phe Gln Glu Ala Gln Lys Leu Gln Ala
    50                  55                  60                  
Met His Arg Glu Lys Tyr Pro Ile Ile Tyr Lys Tyr Arg Pro Arg Arg
65                  70                  75                  80  
Lys Ala Ile Ile
                


<210> 92
<211> 62
<212> DNA
<213> Unknown

<220> 
<223> recognition sequence within HMG domain of Sry

<400> 92
ccccatgaat gcatttatgg tgtggtcccg tggtgagagg cacaagttgg cccagcagaa 60
tc                                                                62

<210> 93
<211> 62
<212> DNA
<213> Unknown

<220> 
<223> recognition sequence within HMG domain of Sry

<400> 93
gattctgctg ggccaacttg tgcctctcac cacgggacca caccataaat gcattcatgg 60
gg                                                                62

<210> 94
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> targeted mutation allele 1 of Sry with deletions
      induced by TALENS

<400> 94
ccccatgaat gcagcagaat c                                           21

<210> 95
<211> 51
<212> DNA
<213> Unknown

<220> 
<223> targeted mutation allele 2 of Sry with deletions
      induced by TALENS

<400> 95
ccccatgaat gcatttatgg tgtgagaggc acaagttggc ccagcagaat c          51

<210> 96
<211> 47
<212> DNA
<213> Unknown

<220> 
<223> targeted mutation allele 3 of Sry with deletions
      induced by TALENS

<400> 96
ccccatgaat gcatttatgg tgtggcacaa gttggcccag cagaatc               47

<210> 97
<211> 28
<212> DNA
<213> Unknown

<220> 
<223> targeting site of Sox2 codon

<400> 97
gtcgcacatg ggcaaacctg agggctgg                                    28

<210> 98
<211> 35
<212> DNA
<213> Unknown

<220> 
<223> targeting site of Sox2 codon

<400> 98
gcacactgcc cctgtcgcac atgtgagggc tggac                            35

<210> 99
<211> 34
<212> DNA
<213> Unknown

<220> 
<223> p2A-mCherry reporter sequence

<400> 99
ctccaccagg tgaaatatga gacttacgca acat                             34

<210> 100
<211> 34
<212> DNA
<213> Unknown

<220> 
<223> p2A-mCherry reporter sequence

<400> 100
atgttgcgta agtctcatat ttcacctggt ggag                             34

<210> 101
<211> 35
<212> DNA
<213> Unknown

<220> 
<223> eGFP sequence

<400> 101
aaagaagctc agtgatgctg ttgatcagga gcctg                            35

<210> 102
<211> 38
<212> DNA
<213> Unknown

<220> 
<223> targeting site in intron 2 of Mecp2

<400> 102
tacagtatgc tagcataact agttatccta gggaagtt                         38

<210> 103
<211> 34
<212> DNA
<213> Unknown

<220> 
<223> targeting site in intron 2 of Mecp2

<400> 103
gcttgaccca aggatacagt atcctaggga agtt                             34

<210> 104
<211> 33
<212> DNA
<213> Unknown

<220> 
<223> targeting site in intron 3 of Mecp2

<400> 104
gacccccaag tactagacct cactccttca gtt                              33

<210> 105
<211> 33
<212> DNA
<213> Unknown

<220> 
<223> targeting site in intron 3 of Mecp2

<400> 105
aactgaagga gtgaggtcta gtacttgggg gtc                              33

<210> 106
<211> 37
<212> DNA
<213> Unknown

<220> 
<223> R1 oligo donor sequence

<400> 106
ggtctagtat aactagttat gaattcactt gggggtc                          37

<210> 107
<211> 65
<212> DNA
<213> Unknown

<220> 
<223> WT sequence of exon 3 in mice

<400> 107
gcttgaccca aggatacagt atcctaggga agttccccca agtactagac ctcactcctt 60
cagtt                                                             65

<210> 108
<211> 67
<212> DNA
<213> Unknown

<220> 
<223> WT sequence of exon 3 in mice

<400> 108
aactgaagga gtgaggtcta gtacttgggg gtcaacttcc ctaggatact gtatccttgg 60
gtcaagc                                                           67

<210> 109
<211> 40
<212> DNA
<213> Unknown

<220> 
<223> mutant sequence of exon 3 in mice

<400> 109
gcttgaccca aggatacagt aagacctcac tccttcagtt                       40

<210> 110
<211> 40
<212> DNA
<213> Unknown

<220> 
<223> mutant sequence of exon 3 in mice

<400> 110
gcttgaccca aggatacaac tagacctcac tccttcagtt                       40

<210> 111
<211> 32
<212> DNA
<213> Unknown

<220> 
<223> mutant sequence of exon 3 in mice

<400> 111
gcttgaccca aggatacctc actccttcag tt                               32

<210> 112
<211> 43
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence in exon 4 of Tet1

<400> 112
agtgtggctg ctgtcaggga gctcatggag actaggtgag gaa                   43

<210> 113
<211> 36
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence in exon 4 of Tet1

<400> 113
gtcagggagg aattcataac tttatctcat ggagac                           36

<210> 114
<211> 44
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence in exon 3 of Tet2

<400> 114
cacgtgaaag tgccaacaga tatccaggct gcagaatcgg agaa                  44

<210> 115
<211> 36
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence in exon 3 of Tet2

<400> 115
ccaacagatg aattcataac tttatatcca ggctgc                           36

<210> 116
<211> 36
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence at Mecp2 intron 2

<400> 116
tacagtatgc tagcataact ttatcctagg gaagtt                           36

<210> 117
<211> 34
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence at Mecp2 intron 2

<400> 117
gcttgaccca aggatacagt atcctaggga agtt                             34

<210> 118
<211> 34
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence at Mecp2 intron 2

<400> 118
aaatctttgg gccccagctt gacccaagga taca                             34

<210> 119
<211> 40
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence at Mecp2 intron 2

<400> 119
ccccagcttg agctagcata actttatccc aaggatacag                       40

<210> 120
<211> 42
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence at Mecp2 intron 2

<400> 120
gtggtggatt aggaattcat aactttatgt ctgggtacca ac                    42

<210> 121
<211> 33
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence at Mecp2 intron 3

<400> 121
gaatgtttgg tggtggatta ggtctgggta cca                              33

<210> 122
<211> 33
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence at Mecp2 intron 3

<400> 122
gacccccaag tactagacct cactccttca gtt                              33

<210> 123
<211> 33
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence at Mecp2 intron 3

<400> 123
aactgaagga gtgaggtcta gtacttgggg gtc                              33

<210> 124
<211> 37
<212> DNA
<213> Unknown

<220> 
<223> targeting sequence at Mecp2 intron 3

<400> 124
ggtctagtat aactagttat gaattcactt gggggtc                          37

<210> 125
<211> 13
<212> PRT
<213> Unknown

<220> 
<223> activation peptide from VP16

<400> 125
Ala Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Pro
 1               5                  10              


<210> 126
<211> 11
<212> PRT
<213> Unknown

<220> 
<223> activation peptide from VP16

<400> 126
Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu
 1               5                  10      


<210> 127
<211> 45
<212> DNA
<213> Unknown

<220> 
<223> Talen recognition sequence of Sry pair1

<400> 127
tggcccagca gaatcccagc atgcaaaata cagagatcag caagc                 45

<210> 128
<211> 45
<212> DNA
<213> Unknown

<220> 
<223> Talen recognition sequence of Sry pair1

<400> 128
gcttgctgat ctctgtattt tgcatgctgg gattctgctg ggcca                 45

<210> 129
<211> 28
<212> PRT
<213> Unknown

<220> 
<223> amino acid sequence of RVD

<400> 129
Asn Gly Asn Asn Asn Asn His Asp His Asp His Asp Asn Ile Asn Asn
 1               5                  10                  15      
His Asp Asn Ile Asn Asn Asn Ile Asn Ile Asn Gly
            20                  25              


<210> 130
<211> 32
<212> PRT
<213> Unknown

<220> 
<223> amino acid sequence of RVD

<400> 130
Asn Asn His Asp Asn Gly Asn Gly Asn Asn His Asp Asn Gly Asn Asn
 1               5                  10                  15      
Asn Ile Asn Gly His Asp Asn Gly His Asp Asn Gly Asn Asn Asn Gly
            20                  25                  30          


<210> 131
<211> 50
<212> DNA
<213> Unknown

<220> 
<223> Talen recognition sequence of Sry2 Pair 2

<400> 131
gaatgcattt atggtgtggt cccgtggtga gaggcacaag ttggcccagc            50

<210> 132
<211> 50
<212> DNA
<213> Unknown

<220> 
<223> Talen recognition sequence of Sry2 Pair 2

<400> 132
gctgggccaa cttgtgcctc tcaccacggg accacaccat aaatgcattc            50

<210> 133
<211> 34
<212> PRT
<213> Unknown

<220> 
<223> amino acid sequence of RVD

<400> 133
Asn Asn Asn Ile Asn Ile Asn Gly Asn Asn His Asp Asn Ile Asn Gly
 1               5                  10                  15      
Asn Gly Asn Gly Asn Ile Asn Gly Asn Asn Asn Asn Asn Gly Asn Asn
            20                  25                  30          
Asn Gly
        


<210> 134
<211> 38
<212> PRT
<213> Unknown

<220> 
<223> amino acid sequence of RVD

<400> 134
Asn Asn His Asp Asn Gly Asn Asn Asn Asn Asn Asn His Asp His Asp
 1               5                  10                  15      
Asn Ile Asn Ile His Asp Asn Gly Asn Gly Asn Asn Asn Gly Asn Asn
            20                  25                  30          
His Asp His Asp Asn Gly
        35              


<210> 135
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of CRISPR sgRNAs

<220> 
<221> misc_feature   
<222> 1, 2, 3, 4, 5, 6, 7
<223> n = A,T,C or G

<400> 135
nnnnnnnmmm mmmmmmmmmm                                             20

<210> 136
<211> 16
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of CRISPR sgRNAs

<400> 136
mmmmmmmmmm mmmagg                                                 16

<210> 137
<211> 16
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of CRISPR sgRNAs

<400> 137
mmmmmmmmmm mmmcgg                                                 16

<210> 138
<211> 16
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of CRISPR sgRNAs

<400> 138
mmmmmmmmmm mmmggg                                                 16

<210> 139
<211> 16
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of CRISPR sgRNAs

<400> 139
mmmmmmmmmm mmmtgg                                                 16

<210> 140
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of Tet1

<400> 140
ggctgctgtc agggagctca tgg                                         23

<210> 141
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of Tet1

<400> 141
ctgtttggtc agggagctca agg                                         23

<210> 142
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of Tet1

<400> 142
gggccaagtc agggagctca agg                                         23

<210> 143
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of Tet1

<400> 143
gtttagtgtc agggagctca ggg                                         23

<210> 144
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of Tet2

<400> 144
gaaagtgcca acagatatcc agg                                         23

<210> 145
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of Tet2

<400> 145
gcaaagacca acagatatcc agg                                         23

<210> 146
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of Tet2

<400> 146
aggaaaccca acagatatcc cgg                                         23

<210> 147
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of Tet2

<400> 147
ccacctccca acagatatcc tgg                                         23

<210> 148
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> potential target sequence of Tet2

<400> 148
gagataacca acagatatcc tgg                                         23

<210> 149
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide to clone sgRNA for Tet1

<400> 149
caccggctgc tgtcagggag ctca                                        24

<210> 150
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide to clone sgRNA for Tet1

<400> 150
aaactgagct ccctgacagc agcc                                        24

<210> 151
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide to clone sgRNA for Tet2

<400> 151
caccgaaagt gccaacagat atcc                                        24

<210> 152
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide to clone sgRNA for Tet2

<400> 152
aaacggatat ctgttggcac tttc                                        24

<210> 153
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide to clone sgRNA for Tet3

<400> 153
caccgaagga ggggaagagt tctcg                                       25

<210> 154
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide to clone sgRNA for Tet3

<400> 154
aaaccgagaa ctcttcccct ccttc                                       25

<210> 155
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide to clone sgRNA for Sry

<400> 155
caccgcattt atggtgtggt cccg                                        24

<210> 156
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide to clone sgRNA for Sry

<400> 156
aaaccgggac cacaccataa atgc                                        24

<210> 157
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide to clone sgRNA for Uty

<400> 157
caccgtttct tttcctcatt accta                                       25

<210> 158
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide to clone sgRNA for Uty

<400> 158
aaactaggta atgaggaaaa gaaac                                       25

<210> 159
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for surveyor assay and
      RFLP of Tet1

<400> 159
ttgttctctc ctctgactgc                                             20

<210> 160
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for surveyor assay and
      RFLP of Tet1

<400> 160
tgattgatca aataggcctg c                                           21

<210> 161
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for surveyor assay and
      RFLP of Tet2

<400> 161
cagatgctta ggccaatcaa g                                           21

<210> 162
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for surveyor assay and
      RFLP of Tet2

<400> 162
agaagcaaca cacatgaaga tg                                          22

<210> 163
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for surveyor assay and
      RFLP of Tet3

<400> 163
ccacctctga gcgcagagtg                                             20

<210> 164
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for surveyor assay and
      RFLP of Tet3

<400> 164
gatgaacaca gttcctgaca g                                           21

<210> 165
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for surveyor assay and
      RFLP of Sry

<400> 165
gtctgtcttt gtctgtctgt c                                           21

<210> 166
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for surveyor assay and
      RFLP of Sry

<400> 166
gggtatttct ctctgtgtag g                                           21

<210> 167
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for surveyor assay and
      RFLP of Uty

<400> 167
gagttcttct tgcgttcacc                                             20

<210> 168
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for surveyor assay and
      RFLP of Uty

<400> 168
aatgagcact ttcagagtag g                                           21

<210> 169
<211> 44
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for making template of
      Cas9

<400> 169
taatacgact cactataggg agaatggact ataaggacca cgac                  44

<210> 170
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for making template of
      Cas9

<400> 170
gcgagctcta ggaattctta c                                           21

<210> 171
<211> 37
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for making template of
      Tet1

<400> 171
ttaatacgac tcactatagg ctgctgtcag ggagctc                          37

<210> 172
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for making template of
      Tet1

<400> 172
aaaagcaccg actcggtgcc                                             20

<210> 173
<211> 39
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for making template of
      Tet2

<400> 173
ttaatacgac tcactatagg aaagtgccaa cagatatcc                        39

<210> 174
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for making template of
      Tet2

<400> 174
aaaagcaccg actcggtgcc                                             20

<210> 175
<211> 40
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for making template of
      Tet3

<400> 175
ttaatacgac tcactatagg aaggagggga agagttctcg                       40

<210> 176
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for making template of
      Tet3

<400> 176
aaaagcaccg actcggtgcc                                             20

<210> 177
<211> 126
<212> DNA
<213> Unknown

<220> 
<223> oligonucleotide for HDR-mediated repiar for Tet1

<400> 177
aaagaaaaag gcccatatta tacacacctt ggggcaggac caagtgtggc tgctgtcagg 60
gaattcatgg agactaggtg aggaactctg cttcccgcta acccattctt cccggtgacc 120
tggctc                                                            126

<210> 178
<211> 126
<212> DNA
<213> Unknown

<220> 
<223> oligonucleotide for HDR-mediated repiar for Tet2

<400> 178
tcactctgtg actataaggc tctgactctc aagtcacaga aacacgtgaa agtgccaaca 60
gaattccagg ctgcagaatc ggagaaccac gcccgagctg cagagcctca agcaaccaaa 120
agcaca                                                            126

<210> 179
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> guide sequence targeting TetO

<400> 179
caccgctttt ctctatcact gata                                        24

<210> 180
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> guide sequence targeting TetO

<400> 180
aaactatcag tgatagagaa aagc                                        24

<210> 181
<211> 34
<212> DNA
<213> Unknown

<220> 
<223> forward primer for mCdk9

<400> 181
aaaaaaggcc ggccatggcc aagcagtacg actc                             34

<210> 182
<211> 35
<212> DNA
<213> Unknown

<220> 
<223> reverse primer for mCdk9

<400> 182
aaaaaagaat tctcagaaga cacgttcaaa ttccg                            35

<210> 183
<211> 37
<212> DNA
<213> Unknown

<220> 
<223> forward primer for mCyct

<400> 183
aaaaaaggcc ggccatggag ggagagagga agaacaa                          37

<210> 184
<211> 40
<212> DNA
<213> Unknown

<220> 
<223> reverse primer for mCycT

<400> 184
aaaaaagaat tctcacttag gaagaggtgg aagtggtgga                       40

<210> 185
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward primer for mGapdh

<400> 185
tgtgtccgtc gtggatctga                                             20

<210> 186
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse primer for mGapdh

<400> 186
cctgcttcac caccttcttg a                                           21

<210> 187
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> forward primer for mNanog

<400> 187
ttgcttacaa gggtctgcta ct                                          22

<210> 188
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse primer for mNanog

<400> 188
actggtagaa gaatcagggc t                                           21

<210> 189
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of Tet binding site-sgTet

<400> 189
gcttttctct atcactgata ggg                                         23

<210> 190
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of Tet binding site - sgTet

<400> 190
caccgctttt ctctatcact gata                                        24

<210> 191
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of Tet binding site - sgTet

<400> 191
aaactatcag tgatagagaa aagc                                        24

<210> 192
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mNanog promoter - sgmNanog-1

<400> 192
gtaatgcaaa agaagctgta agg                                         23

<210> 193
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mNanog promoter - sgmNanog-1

<400> 193
caccgtaatg caaaagaagc tgta                                        24

<210> 194
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mNanog promoter - sgmNanog-1

<400> 194
aaactacagc ttcttttgca ttac                                        24

<210> 195
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mNanog promoter - sgmNanog-2

<400> 195
gatctctagt gggaagtttc agg                                         23

<210> 196
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mNanog promoter - sgmNanog-2

<400> 196
caccgatctc tagtgggaag tttc                                        24

<210> 197
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mNanog promoter - sgmNanog-2

<400> 197
aaacgaaact tcccactaga gatc                                        24

<210> 198
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mNanog promoter - sgmNanog-3

<400> 198
gctcttcaca ttgggaaacc tgg                                         23

<210> 199
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mNanog promoter - sgmNanog-3

<400> 199
caccgctctt cacattggga aacc                                        24

<210> 200
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mNanog promoter - sgmNanog-3

<400> 200
aaacggtttc ccaatgtgaa gagc                                        24

<210> 201
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mNanog promoter - sgmNanog-4

<400> 201
gagtgtttaa attaatgtag agg                                         23

<210> 202
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mNanog promoter - sgmNanog-4

<400> 202
caccgagtgt ttaaattaat gtag                                        24

<210> 203
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mNanog promoter - sgmNanog-4

<400> 203
aaacctacat taatttaaac actc                                        24

<210> 204
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mNanog promoter - sgmNanog-5

<400> 204
gagtttcacg tacccgagac tgg                                         23

<210> 205
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mNanog promoter - sgmNanog-5

<400> 205
caccgagttt cacgtacccg agac                                        24

<210> 206
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mNanog promoter - sgmNanog-5

<400> 206
aaacgtctcg ggtacgtgaa actc                                        24

<210> 207
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mNanog promoter - sgmNanog-6

<400> 207
gcttctgtgt ataagcagag agg                                         23

<210> 208
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mNanog promoter - sgmNanog-6

<400> 208
caccgcttct gtgtataagc agag                                        24

<210> 209
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mNanog promoter - sgmNanog-6

<400> 209
aaacctctgc ttatacacag aagc                                        24

<210> 210
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mNanog promoter - sgmNanog-7

<400> 210
gcgttaaaaa gccgcacttt tgg                                         23

<210> 211
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mNanog promoter - sgmNanog-7

<400> 211
caccgcgtta aaaagccgca cttt                                        24

<210> 212
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mNanog promoter - sgmNanog-7

<400> 212
aaacaaagtg cggcttttta acgc                                        24

<210> 213
<211> 14
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mNanog promoter - sgmNanog-8

<400> 213
gtctgtagaa agaa                                                   14

<210> 214
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mNanog promoter - sgmNanog-8

<400> 214
caccgtctgt agaaagaatg gaag                                        24

<210> 215
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mNanog promoter - sgmNanog-8

<400> 215
aaaccttcca ttctttctac agac                                        24

<210> 216
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of Target_Sox2_Stop

<400> 216
tgcccctgtc gcacatgtga ggg                                         23

<210> 217
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT1_Sox2_Stop

<400> 217
tacccttgtt gcacatgtga agg                                         23

<210> 218
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT2_Sox2_Stop

<400> 218
ttcccatgta gcacatgtga ggg                                         23

<210> 219
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT3_Sox2_Stop

<400> 219
tccccctgtc acacatgtgg tgg                                         23

<210> 220
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT4_Sox2_Stop

<400> 220
tgcacctgtg gcacatgtgg ggg                                         23

<210> 221
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT5_Sox2_Stop

<400> 221
tgccacagtt gcacatgtga ggg                                         23

<210> 222
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT6_Sox2_Stop

<400> 222
tgccactgtt gcaaatgtga ggg                                         23

<210> 223
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT7_Sox2_Stop

<400> 223
tgcctctgtc acagatgtga cgg                                         23

<210> 224
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT8_Sox2_Stop

<400> 224
tgcctctgtc tcacatgtgc tgg                                         23

<210> 225
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT9_Sox2_Stop

<400> 225
tgcctctgtc gctcatggga tgg                                         23

<210> 226
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT10_Sox2_Stop

<400> 226
tgcccatgtc ccacatggga tgg                                         23

<210> 227
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT11_Sox2_Stop

<400> 227
tgcccctctg tcacatgtga agg                                         23

<210> 228
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT12_Sox2_Stop

<400> 228
tgcccctgtc atacatgtgg agg                                         23

<210> 229
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT13_Sox2_Stop

<400> 229
tgcccctgtc tcccatgtgc tgg                                         23

<210> 230
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of Target_Nanog_Stop

<400> 230
cgtaagtctc atatttcacc tgg                                         23

<210> 231
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT1_Nanog_Stop

<400> 231
tgtaagtctc atatttcacc tgg                                         23

<210> 232
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT2_Nanog_Stop

<400> 232
cccaagtctc atttttcacc agg                                         23

<210> 233
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT3_Nanog_Stop

<400> 233
gataaggatc atatttcacc cgg                                         23

<210> 234
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT4_Nanog_Stop

<400> 234
tgcaattttc atatttcacc tgg                                         23

<210> 235
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT5_Nanog_Stop

<400> 235
ggtcatcctc atatttcacc agg                                         23

<210> 236
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT6_Nanog_Stop

<400> 236
tgttattcac atatttcacc tgg                                         23

<210> 237
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT7_Nanog_Stop

<400> 237
tgtgagtagc atatttcacc tgg                                         23

<210> 238
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT8_Nanog_Stop

<400> 238
tgtaaataac atatttcacc cgg                                         23

<210> 239
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT9_Nanog_Stop

<400> 239
catagagctc atatttcacc agg                                         23

<210> 240
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of Target_Mecp2_L2

<400> 240
cccaaggata cagtatccta ggg                                         23

<210> 241
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT1_Mecp2_L2

<400> 241
cccaaggatg ctttatccta agg                                         23

<210> 242
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT2_Mecp2_L2

<400> 242
cccatggata gagtagccta agg                                         23

<210> 243
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT3_Mecp2_L2

<400> 243
cccaagaata cagtgtgcta agg                                         23

<210> 244
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT4_Mecp2_L2

<400> 244
cccaaggaca caggatccca agg                                         23

<210> 245
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of Target_Mecp2_R1

<400> 245
aggagtgagg tctagtactt ggg                                         23

<210> 246
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT1_Mecp2_R1

<400> 246
aggggtgagt tctagtactt cgg                                         23

<210> 247
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT2_Mecp2_R1

<400> 247
tggagtgagg tcttgtactt ggg                                         23

<210> 248
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> off target sequence of OT3_Mecp2_R1

<400> 248
aggagtctgg gctagtactt ggg                                         23

<210> 249
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Tet1

<400> 249
caccggctgc tgtcagggag ctca                                        24

<210> 250
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Tet1

<400> 250
aaactgagct ccctgacagc agcc                                        24

<210> 251
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Tet2

<400> 251
caccgaaagt gccaacagat atcc                                        24

<210> 252
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Tet2

<400> 252
aaacggatat ctgttggcac tttc                                        24

<210> 253
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Sox2

<400> 253
caccgtgccc ctgtcgcaca tgtga                                       25

<210> 254
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Sox2

<400> 254
aaactcacat gtgcgacagg ggcac                                       25

<210> 255
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Nanog

<400> 255
caccgcgtaa gtctcatatt tcacc                                       25

<210> 256
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Nanog

<400> 256
aaacggtgaa atatgagact tacgc                                       25

<210> 257
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Oct4

<400> 257
caccgctcag tgatgctgtt gatc                                        24

<210> 258
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Oct4

<400> 258
aaacgatcaa cagcatcact gagc                                        24

<210> 259
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Mecp2 L1

<400> 259
caccgttggg ccccagcttg accca                                       25

<210> 260
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Mecp2 L1

<400> 260
aaactgggtc aagctggggc ccaac                                       25

<210> 261
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Mecp2 L2

<400> 261
caccgcccaa ggatacagta tccta                                       25

<210> 262
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Mecp2 L2

<400> 262
aaactaggat actgtatcct tgggc                                       25

<210> 263
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Mecp2 R1

<400> 263
caccgaggag tgaggtctag tactt                                       25

<210> 264
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Mecp2 R1

<400> 264
aaacaagtac tagacctcac tcctc                                       25

<210> 265
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Mecp2 R2

<400> 265
caccgtttgg tggtggatta ggtct                                       25

<210> 266
<211> 25
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Mecp2 R2

<400> 266
aaacagacct aatccaccac caaac                                       25

<210> 267
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Tet1

<400> 267
ttgttctctc ctctgactgc                                             20

<210> 268
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Tet1

<400> 268
tgattgatca aataggcctg c                                           21

<210> 269
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Tet2

<400> 269
cagatgctta ggccaatcaa g                                           21

<210> 270
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Tet2

<400> 270
agaagcaaca cacatgaaga tg                                          22

<210> 271
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Sox2

<400> 271
acatgatcag catgtacctc c                                           21

<210> 272
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Sox2

<400> 272
taatttggat gggattggtg g                                           21

<210> 273
<211> 18
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for V5

<400> 273
acatgggcaa gcccatcc                                               18

<210> 274
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Mecp2 L1, L2

<400> 274
aatgtgccac tttaacagca c                                           21

<210> 275
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Mecp2 L1, L2

<400> 275
ttctgatgtt tctgctttgc c                                           21

<210> 276
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Mecp2 R1, R2

<400> 276
aagcatgagc cactacaacc                                             20

<210> 277
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Mecp2 R1, R2

<400> 277
cttgctcaga agccaaaaca g                                           21

<210> 278
<211> 44
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Cas9

<400> 278
taatacgact cactataggg agaatggact ataaggacca cgac                  44

<210> 279
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Cas9

<400> 279
gcgagctcta ggaattctta c                                           21

<210> 280
<211> 37
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Tet1 sgRNA

<400> 280
ttaatacgac tcactatagg ctgctgtcag ggagctc                          37

<210> 281
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Tet1 sgRNA

<400> 281
aaaagcaccg actcggtgcc                                             20

<210> 282
<211> 39
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Tet2 sgRNA

<400> 282
ttaatacgac tcactatagg aaagtgccaa cagatatcc                        39

<210> 283
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Tet2 sgRNA

<400> 283
aaaagcaccg actcggtgcc                                             20

<210> 284
<211> 39
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Sox2 sgRNA

<400> 284
ttaatacgac tcactatagt gcccctgtcg cacatgtga                        39

<210> 285
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Sox2 sgRNA

<400> 285
aaaagcaccg actcggtgcc                                             20

<210> 286
<211> 39
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Nanog sgRNA

<400> 286
ttaatacgac tcactatagc gtaagtctca tatttcacc                        39

<210> 287
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Nanog sgRNA

<400> 287
aaaagcaccg actcggtgcc                                             20

<210> 288
<211> 39
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Oct4

<400> 288
ttaatacgac tcactatagg ctcagtgatg ctgttgatc                        39

<210> 289
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Oct4

<400> 289
aaaagcaccg actcggtgcc                                             20

<210> 290
<211> 39
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Mecp2-L1 sgRNA

<400> 290
ttaatacgac tcactatagt tgggccccag cttgaccca                        39

<210> 291
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Mecp2-L1 sgRNA

<400> 291
aaaagcaccg actcggtgcc                                             20

<210> 292
<211> 39
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Mecp2-L2 sgRNA

<400> 292
ttaatacgac tcactatagc ccaaggatac agtatccta                        39

<210> 293
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Mecp2-L2 sgRNA

<400> 293
aaaagcaccg actcggtgcc                                             20

<210> 294
<211> 39
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Mecp2-R1 sgRNA

<400> 294
ttaatacgac tcactataga ggagtgaggt ctagtactt                        39

<210> 295
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Mecp2-R1 sgRNA

<400> 295
aaaagcaccg actcggtgcc                                             20

<210> 296
<211> 39
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide sequence for cloning sgRNA
      for Mecp2-R2 sgRNA

<400> 296
ttaatacgac tcactatagt ttggtggtgg attaggtct                        39

<210> 297
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide sequence for cloning sgRNA
      for Mecp2-R2 sgRNA

<400> 297
aaaagcaccg actcggtgcc                                             20

<210> 298
<211> 160
<212> DNA
<213> Unknown

<220> 
<223> Tet1-loxP sequence used for HDR-mediated repair

<400> 298
gaaaaaggcc catattatac acaccttggg gcaggaccaa gtgtggctgc tgtcagggag 60
gaattcataa cttcgtataa tgtatgctat acgaagttat ctcatggaga ctaggtgagg 120
aactctgctt cccgctaacc cattcttccc ggtgacctgg                       160

<210> 299
<211> 160
<212> DNA
<213> Unknown

<220> 
<223> Tet2-loxP sequence used for HDR-mediated repair

<400> 299
ctctgtgact ataaggctct gactctcaag tcacagaaac acgtgaaagt gccaacagat 60
gaattcataa cttcgtataa tgtatgctat acgaagttat atccaggctg cagaatcgga 120
gaaccacgcc cgagctgcag agcctcaagc aaccaaaagc                       160

<210> 300
<211> 162
<212> DNA
<213> Unknown

<220> 
<223> Sox2-v5 sequence used for HDR-mediated repair

<400> 300
taccagagcg gcccggtgcc cggcacggcc attaacggca cactgcccct gtcgcacatg 60
ggcaagccca tccccaaccc cctgctgggc ctggacagca cctgagggct ggactgcgaa 120
ctggagaagg ggagagattt tcaaagagat acaagggaat tg                    162

<210> 301
<211> 160
<212> DNA
<213> Unknown

<220> 
<223> Mecp2-L1-loxP sequence used for HDR-mediated
      repair

<400> 301
tgtttgacca atatcaccag caacctaaag ctgttaagaa atctttgggc cccagcttga 60
gctagcataa cttcgtataa tgtatgctat acgaagttat cccaaggata cagtatccta 120
gggaagttac caaaatcaga gatagtatgc agcagccagg                       160

<210> 302
<211> 160
<212> DNA
<213> Unknown

<220> 
<223> Mecp2-L2-loxP sequence used for HDR-mediated
      repair

<400> 302
ccagcaacct aaagctgtta agaaatcttt gggccccagc ttgacccaag gatacagtat 60
gctagcataa cttcgtataa tgtatgctat acgaagttat cctagggaag ttaccaaaat 120
cagagatagt atgcagcagc caggggtctc atgtgtggca                       160

<210> 303
<211> 160
<212> DNA
<213> Unknown

<220> 
<223> Mecp2-R1-loxP sequence used for HDR-mediated
      repair

<400> 303
ccactcctct gtactccctg gcttttccac aatccttaaa ctgaaggagt gaggtctagt 60
ataacttcgt atagcataca ttatacgaag ttatgaattc acttgggggt cattgggcta 120
gactgaatat ctttggttgg tacccagacc taatccacca                       160

<210> 304
<211> 160
<212> DNA
<213> Unknown

<220> 
<223> Mecp2-R2-loxP sequence used for HDR-mediated
      repair

<400> 304
ccaaaaaggc tggacaccat gccttggtta aaatggagga atgttttggt ggtggattag 60
gaattcataa cttcgtataa tgtatgctat acgaagttat gtctgggtac caaccaaaga 120
tattcagtct agcccaatga cccccaagta ctagacctca                       160

<210> 305
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT1_Sox2_Stop

<400> 305
atgacatgac ctaagtaaac cc                                          22

<210> 306
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT1_Sox2_Stop

<400> 306
ctccactctg tactaggcac                                             20

<210> 307
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT2_Sox2_Stop

<400> 307
tgatggtttt tggtgactgc c                                           21

<210> 308
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT2_Sox2_Stop

<400> 308
gacagatcat agatagaaaa ttg                                         23

<210> 309
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT3_Sox2_Stop

<400> 309
aaactgaggc acagagtctg                                             20

<210> 310
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT3_Sox2_Stop

<400> 310
gtgacgaagc cactttgacc                                             20

<210> 311
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT4_Sox2_Stop

<400> 311
caccttaggt tcatggcatt c                                           21

<210> 312
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT4_Sox2_Stop

<400> 312
gatggatcag tgattaagag c                                           21

<210> 313
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT5_Sox2_Stop

<400> 313
accatgatgg actgtaccat c                                           21

<210> 314
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT5_Sox2_Stop

<400> 314
catggacgtc attactagat g                                           21

<210> 315
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT6_Sox2_Stop

<400> 315
ttcctcgaag atgaaatgat t                                           21

<210> 316
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT6_Sox2_Stop

<400> 316
cagtgtgcag actctgagag                                             20

<210> 317
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT7_Sox2_Stop

<400> 317
atgtgccaca caaggcaggc                                             20

<210> 318
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT7_Sox2_Stop

<400> 318
gcaaaacctc tgaaagttga c                                           21

<210> 319
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT8_Sox2_Stop

<400> 319
ttcctgtcct ggcttccttc                                             20

<210> 320
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT8_Sox2_Stop

<400> 320
gcactagttg tcacgtgatg                                             20

<210> 321
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT9_Sox2_Stop

<400> 321
gactcagatt tccaagccat g                                           21

<210> 322
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT9_Sox2_Stop

<400> 322
acatctctga gctctaagcc                                             20

<210> 323
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT10_Sox2_Stop

<400> 323
tgccatgtgc tgtgttcacc                                             20

<210> 324
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT10_Sox2_Stop

<400> 324
ttgatattta agacagggtc tc                                          22

<210> 325
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT11_Sox2_Stop

<400> 325
gtaaggaatg taagaactct tg                                          22

<210> 326
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT11_Sox2_Stop

<400> 326
aattctcaac tgaggaatac tg                                          22

<210> 327
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT12_Sox2_Stop

<400> 327
tctcagacag aaacgctgtg                                             20

<210> 328
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT12_Sox2_Stop

<400> 328
gacttgatat gccaggatga g                                           21

<210> 329
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT13_Sox2_Stop

<400> 329
agctgacaga agacgatgag                                             20

<210> 330
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT13_Sox2_Stop

<400> 330
taaacccaag caaaggtcat g                                           21

<210> 331
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT1_Nanog_Stop

<400> 331
gctggtgaga tggctcagtg                                             20

<210> 332
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT1_Nanog_Stop

<400> 332
gtcttaacct gcttatagca ac                                          22

<210> 333
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT2_Nanog_Stop

<400> 333
agatcccatt acggatggtt g                                           21

<210> 334
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT2_Nanog_Stop

<400> 334
ggacactcac caatgtttgg                                             20

<210> 335
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT3_Nanog_Stop

<400> 335
tagattatct agtgtgttcc ac                                          22

<210> 336
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT3_Nanog_Stop

<400> 336
agtttcagtg ctcagagcac                                             20

<210> 337
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT4_Nanog_Stop

<400> 337
gacactttct aagtgggctt g                                           21

<210> 338
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT4_Nanog_Stop

<400> 338
gttaagggac agtgaatatc c                                           21

<210> 339
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT5_Nanog_Stop

<400> 339
tcccatctac cctctgactg                                             20

<210> 340
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT5_Nanog_Stop

<400> 340
gcctgaagaa aagaaggtcc                                             20

<210> 341
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT6_Nanog_Stop

<400> 341
tctgaggtga gcaaagcatg                                             20

<210> 342
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT6_Nanog_Stop

<400> 342
aatccaccat gtcttccgtg                                             20

<210> 343
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT7_Nanog_Stop

<400> 343
caattttctc agtgaggtag g                                           21

<210> 344
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT7_Nanog_Stop

<400> 344
cttgttcagt gcattgctgc                                             20

<210> 345
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT8_Nanog_Stop

<400> 345
tctcttcaga aaagagtagg c                                           21

<210> 346
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT8_Nanog_Stop

<400> 346
gttggcaact gcactctgtg                                             20

<210> 347
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT9_Nanog_Stop

<400> 347
agctcatgca tgctgagctg                                             20

<210> 348
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT9_Nanog_Stop

<400> 348
aacttcaagt ggaactgctt g                                           21

<210> 349
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT1_Mecp2_Left2

<400> 349
cacacacaca ctgaataaaa tg                                          22

<210> 350
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT1_Mecp2_Left2

<400> 350
aagctggctt tgagcaggac                                             20

<210> 351
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT2_Mecp2_Left2

<400> 351
tagtcactta tgtttactcc tc                                          22

<210> 352
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT2_Mecp2_Left2

<400> 352
gtgatgccag cagttggcag                                             20

<210> 353
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT3_Mecp2_Left2

<400> 353
tcactttccc tcagtactcc                                             20

<210> 354
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT3_Mecp2_Left2

<400> 354
caagtatcat tctctgaaca ag                                          22

<210> 355
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT4_Mecp2_Left2

<400> 355
gaactttgag acagggtctc                                             20

<210> 356
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT4_Mecp2_Left2

<400> 356
gacagagcag cttggccttc                                             20

<210> 357
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT1_Mecp2_Right1

<400> 357
gcagcaccag tggaatatta c                                           21

<210> 358
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT1_Mecp2_Right1

<400> 358
gcctattgat gaatctgccc                                             20

<210> 359
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT2_Mecp2_Right1

<400> 359
acagatgcag ccactcacag                                             20

<210> 360
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT2_Mecp2_Right1

<400> 360
gtccaagtca cttctcccac                                             20

<210> 361
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT3_Mecp2_Right1

<400> 361
tccgacaatg gtttatgtct g                                           21

<210> 362
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT3_Mecp2_Right1

<400> 362
agatactagc agtgcagctg                                             20

<210> 363
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT4_Mecp2_Right1

<400> 363
gttcctgctg gttttgtttc g                                           21

<210> 364
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT4_Mecp2_Right1

<400> 364
tagaccaatc tacaaccaca g                                           21

<210> 365
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT5_Mecp2_Right1

<400> 365
tgctgtgaaa ctcaggcatg                                             20

<210> 366
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT5_Mecp2_Right1

<400> 366
cttctaagac aagccagaaa g                                           21

<210> 367
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT6_Mecp2_Right1

<400> 367
cggcataaac ctcccattag                                             20

<210> 368
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT6_Mecp2_Right1

<400> 368
ctctgtgctt gtaaggcaaa c                                           21

<210> 369
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT7_Mecp2_Right1

<400> 369
gccagacaat aattcccaag                                             20

<210> 370
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT7_Mecp2_Right1

<400> 370
ctgatattgc tactgctaac c                                           21

<210> 371
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT8_Mecp2_Right1

<400> 371
ccattgtgaa agtgggatgc                                             20

<210> 372
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT8_Mecp2_Right1

<400> 372
ggctgctctc gtaaacaaaa c                                           21

<210> 373
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT9_Mecp2_Right1

<400> 373
gtcactctca tgtgcaggtg                                             20

<210> 374
<211> 21
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT9_Mecp2_Right1

<400> 374
ctagcacttg ggaagcaaat g                                           21

<210> 375
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> forward oligonucleotide for off-target analysis of
      OT10_Mecp2_Right1

<400> 375
ctaatcacac ttctacaagc tg                                          22

<210> 376
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse oligonucleotide for off-target analysis of
      OT10_Mecp2_Right1

<400> 376
agagaggctc caattgttag                                             20

<210> 377
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> forward primer for Sox2

<400> 377
cacctacagc atgtcctact cg                                          22

<210> 378
<211> 22
<212> DNA
<213> Unknown

<220> 
<223> reverse primer for Sox2

<400> 378
ggttttctcc atgctgtttc tt                                          22

<210> 379
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward primer for IL1RN

<400> 379
gaccctctgg gagaaaatcc                                             20

<210> 380
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse primer for IL1RN

<400> 380
gtccttgcaa gtatccagca                                             20

<210> 381
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward primer for Oct4

<400> 381
gctcgagaag gatgtggtcc                                             20

<210> 382
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse primer for Oct4

<400> 382
cgttgtgcat agtcgctgct                                             20

<210> 383
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> forward primer for GAPDH

<400> 383
cgagatccct ccaaaatcaa                                             20

<210> 384
<211> 20
<212> DNA
<213> Unknown

<220> 
<223> reverse primer for GAPDH

<400> 384
atccacagtc ttctgggtgg                                             20

<210> 385
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence for Tet binding site - sgTetO

<400> 385
gcttttctct atcactgata ggg                                         23

<210> 386
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of Tet binding site - sgTetO

<400> 386
caccgctttt ctctatcact gata                                        24

<210> 387
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of Tet binding site - sgTetO

<400> 387
aaactatcag tgatagagaa aagc                                        24

<210> 388
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mutant version of  sgTetO -
      sgTetOMut

<400> 388
gcttttcttt atcattggta ggg                                         23

<210> 389
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mutant version of  sgTetO -
      sgTetOMut

<400> 389
caccgctttt ctttatcatt ggta                                        24

<210> 390
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mutant version of  sgTetO -
      sgTetOMut

<400> 390
aaactaccaa tgataaagaa aagc                                        24

<210> 391
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mouse Nanog - sgNanog-1

<400> 391
gtaatgcaaa agaagctgta agg                                         23

<210> 392
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mouse Nanog - sgNanog-1

<400> 392
caccgtaatg caaaagaagc tgta                                        24

<210> 393
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mouse Nanog - sgNanog-1

<400> 393
aaactacagc ttcttttgca ttac                                        24

<210> 394
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mouse Nanog - sgNanog-2

<400> 394
gatctctagt gggaagtttc agg                                         23

<210> 395
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mouse Nanog - sgNanog-2

<400> 395
caccgatctc tagtgggaag tttc                                        24

<210> 396
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mouse Nanog - sgNanog-2

<400> 396
aaacgaaact tcccactaga gatc                                        24

<210> 397
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mouse Nanog - sgNanog-3

<400> 397
gtctgtagaa agaatggaag agg                                         23

<210> 398
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mouse Nanog - sgNanog-3

<400> 398
caccgtctgt agaaagaatg gaag                                        24

<210> 399
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mouse Nanog - sgNanog-3

<400> 399
aaaccttcca ttctttctac agac                                        24

<210> 400
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mouse Nanog - sgNanog-4

<400> 400
gctcttcaca ttgggaaacc tgg                                         23

<210> 401
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mouse Nanog - sgNanog-4

<400> 401
caccgctctt cacattggga aacc                                        24

<210> 402
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mouse Nanog - sgNanog-4

<400> 402
aaacggtttc ccaatgtgaa gagc                                        24

<210> 403
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mouse Nanog - sgNanog-5

<400> 403
gcgttaaaaa gccgcacttt tgg                                         23

<210> 404
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mouse Nanog - sgNanog-5

<400> 404
caccgcgtta aaaagccgca cttt                                        24

<210> 405
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mouse Nanog - sgNanog-5

<400> 405
aaacaaagtg cggcttttta acgc                                        24

<210> 406
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mouse Nanog - sgNanog-6

<400> 406
gagtgtttaa attaatgtag agg                                         23

<210> 407
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mouse Nanog - sgNanog-6

<400> 407
caccgagtgt ttaaattaat gtag                                        24

<210> 408
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mouse Nanog - sgNanog-6

<400> 408
aaacctacat taatttaaac actc                                        24

<210> 409
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of mouse Nanog - sgNanog-7

<400> 409
gagtttcacg tacccgagac tgg                                         23

<210> 410
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of mouse Nanog - sgNanog-7

<400> 410
caccgagttt cacgtacccg agac                                        24

<210> 411
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of mouse Nanog - sgNanog-7

<400> 411
aaacgtctcg ggtacgtgaa actc                                        24

<210> 412
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of IL1RN binding site -  sgIL1RN-1

<400> 412
gcacctcaga gagtacaagg agg                                         23

<210> 413
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of IL1RN binding site -  sgIL1RN-1

<400> 413
caccgcacct cagagagtac aagg                                        24

<210> 414
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of IL1RN binding site -  sgIL1RN-1

<400> 414
aaacccttgt actctctgag gtgc                                        24

<210> 415
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of IL1RN binding site -  sgIL1RN-2

<400> 415
gggctgactt gatgccaagc agg                                         23

<210> 416
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of IL1RN binding site -  sgIL1RN-2

<400> 416
caccgggctg acttgatgcc aagc                                        24

<210> 417
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of IL1RN binding site-sgIL1RN-2

<400> 417
aaacgcttgg catcaagtca gccc                                        24

<210> 418
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of IL1RN binding site-sgIL1RN-3

<400> 418
ggtttccagg agggtgactc agg                                         23

<210> 419
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of IL1RN binding site-sgIL1RN-3

<400> 419
caccggtttc caggagggtg actc                                        24

<210> 420
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of IL1RN binding site-sgIL1RN-3

<400> 420
aaacgagtca ccctcctgga aacc                                        24

<210> 421
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of IL1RN binding site-sgIL1RN-4

<400> 421
gggttcttat ctgcgtaaga tgg                                         23

<210> 422
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of IL1RN binding site-sgIL1RN-4

<400> 422
caccgggttc ttatctgcgt aaga                                        24

<210> 423
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of IL1RN binding site-sgIL1RN-4

<400> 423
aaactcttac gcagataaga accc                                        24

<210> 424
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of IL1RN binding site-sgIL1RN-5

<400> 424
gattgggaac aagccagaca agg                                         23

<210> 425
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of IL1RN binding site-sgIL1RN-5

<400> 425
caccgattgg gaacaagcca gaca                                        24

<210> 426
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of IL1RN binding site-sgIL1RN-5

<400> 426
aaactgtctg gcttgttccc aatc                                        24

<210> 427
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of IL1RN binding site-sgIL1RN-6

<400> 427
gatatgcttt tgagggacct agg                                         23

<210> 428
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of IL1RN binding site-sgIL1RN-6

<400> 428
caccgatatg cttttgaggg acct                                        24

<210> 429
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of IL1RN binding site-sgIL1RN-6

<400> 429
aaacaggtcc ctcaaaagca tatc                                        24

<210> 430
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of SOX2 binding site-sgSOX2-1

<400> 430
ggggagagga ggaggggagg cgg                                         23

<210> 431
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of SOX2 binding site-sgSOX2-1

<400> 431
caccggggag aggaggaggg gagg                                        24

<210> 432
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of SOX2 binding site-sgSOX2-1

<400> 432
aaaccctccc ctcctcctct cccc                                        24

<210> 433
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of SOX2 binding site-sgSOX2-2

<400> 433
gagagaggca aactggaatc agg                                         23

<210> 434
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of SOX2 binding site-sgSOX2-2

<400> 434
caccgagaga ggcaaactgg aatc                                        24

<210> 435
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of SOX2 binding site-sgSOX2-2

<400> 435
aaacgattcc agtttgcctc tctc                                        24

<210> 436
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of SOX2 binding site-sgSOX2-3

<400> 436
gcatgtgacg ggggctgtca ggg                                         23

<210> 437
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of SOX2 binding site-sgSOX2-3

<400> 437
caccgcatgt gacgggggct gtca                                        24

<210> 438
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of SOX2 binding site-sgSOX2-3

<400> 438
aaactgacag cccccgtcac atgc                                        24

<210> 439
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of SOX2 binding site-sgSOX2-4

<400> 439
gctgccgggt tttgcatgaa agg                                         23

<210> 440
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of SOX2 binding site-sgSOX2-4

<400> 440
caccgctgcc gggttttgca tgaa                                        24

<210> 441
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of SOX2 binding site-sgSOX2-4

<400> 441
aaacttcatg caaaacccgg cagc                                        24

<210> 442
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of SOX2 binding site-sgSOX2-5

<400> 442
gccggccgcg cgggggaggc cgg                                         23

<210> 443
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of SOX2 binding site-sgSOX2-5

<400> 443
caccgccggc cgcgcggggg aggc                                        24

<210> 444
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of SOX2 binding site-sgSOX2-5

<400> 444
aaacgcctcc cccgcgcggc cggc                                        24

<210> 445
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of SOX2 binding site-sgSOX2-6

<400> 445
ggcaggcgag gagggggagg agg                                         23

<210> 446
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of SOX2 binding site-sgSOX2-6

<400> 446
caccggcagg cgaggagggg gagg                                        24

<210> 447
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of SOX2 binding site-sgSOX2-6

<400> 447
aaaccctccc cctcctcgcc tgcc                                        24

<210> 448
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of SOX2 binding site-sgSOX2-7

<400> 448
gtatcccctc tcgcagcaac agg                                         23

<210> 449
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of SOX2 binding site-sgSOX2-7

<400> 449
caccgtatcc cctctcgcag caac                                        24

<210> 450
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of SOX2 binding site-sgSOX2-7

<400> 450
aaacgttgct gcgagagggg atac                                        24

<210> 451
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of SOX2 binding site-sgSOX2-8

<400> 451
gcagggtact taaatgagga tgg                                         23

<210> 452
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of SOX2 binding site-sgSOX2-8

<400> 452
caccgcaggg tacttaaatg agga                                        24

<210> 453
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of SOX2 binding site-sgSOX2-8

<400> 453
aaactcctca tttaagtacc ctgc                                        24

<210> 454
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of SOX2 binding site-sgSOX2-9

<400> 454
gcagctaagg tgcgggggtg ggg                                         23

<210> 455
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of SOX2 binding site-sgSOX2-9

<400> 455
caccgcagct aaggtgcggg ggtg                                        24

<210> 456
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of SOX2 binding site-sgSOX2-9

<400> 456
aaaccacccc cgcaccttag ctgc                                        24

<210> 457
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of SOX2 binding site-sgSOX2-10

<400> 457
ggctgtccaa ctcgtatttc tgg                                         23

<210> 458
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of SOX2 binding site-sgSOX2-10

<400> 458
caccggctgt ccaactcgta tttc                                        24

<210> 459
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of SOX2 binding site-sgSOX2-10

<400> 459
aaacgaaata cgagttggac agcc                                        24

<210> 460
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of OCT4 binding site-sgOCT4-1

<400> 460
gaaggaaggc gccccaagcc ggg                                         23

<210> 461
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of OCT4 binding site-sgOCT4-1

<400> 461
caccgaagga aggcgcccca agcc                                        24

<210> 462
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of OCT4 binding site-sgOCT4-1

<400> 462
aaacggcttg gggcgccttc cttc                                        24

<210> 463
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of OCT4 binding site-sgOCT4-2

<400> 463
ggtgaaatga gggcttgcga agg                                         23

<210> 464
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of OCT4 binding site-sgOCT4-2

<400> 464
caccggtgaa atgagggctt gcga                                        24

<210> 465
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of OCT4 binding site-sgOCT4-2

<400> 465
aaactcgcaa gccctcattt cacc                                        24

<210> 466
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of OCT4 binding site-sgOCT4-3

<400> 466
ggccccgccc cctggatggg tgg                                         23

<210> 467
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of OCT4 binding site-sgOCT4-3

<400> 467
caccggcccc gccccctgga tggg                                        24

<210> 468
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of OCT4 binding site-sgOCT4-3

<400> 468
aaaccccatc cagggggcgg ggcc                                        24

<210> 469
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of OCT4 binding site-sgOCT4-4

<400> 469
ggggggagaa actgaggcga agg                                         23

<210> 470
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of OCT4 binding site-sgOCT4-4

<400> 470
caccgggggg agaaactgag gcga                                        24

<210> 471
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of OCT4 binding site-sgOCT4-4

<400> 471
aaactcgcct cagtttctcc cccc                                        24

<210> 472
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of OCT4 binding site-sgOCT4-5

<400> 472
ggtggtggca atggtgtctg tgg                                         23

<210> 473
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of OCT4 binding site-sgOCT4-5

<400> 473
caccggtggt ggcaatggtg tctg                                        24

<210> 474
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of OCT4 binding site-sgOCT4-5

<400> 474
aaaccagaca ccattgccac cacc                                        24

<210> 475
<211> 23
<212> DNA
<213> Unknown

<220> 
<223> target sequence of OCT4 binding site-sgOCT4-6

<400> 475
gacacaactg gcgcccctcc agg                                         23

<210> 476
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> forward oligo of OCT4 binding site-sgOCT4-6

<400> 476
caccgacaca actggcgccc ctcc                                        24

<210> 477
<211> 24
<212> DNA
<213> Unknown

<220> 
<223> reverse oligo of OCT4 binding site-sgOCT4-6

<400> 477
aaacggaggg gcgccagttg tgtc                                        24

<210> 478
<211> 1763
<212> PRT
<213> Unknown

<220> 
<223> dCas9VP160-2A-puro

<400> 478
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Gly Ser Gly Arg Ala Asp
        1395                1400                1405            
Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp
    1410                1415                1420                
Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp
1425                1430                1435               1440 
Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met
                1445                1450                1455    
Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser
            1460                1465                1470        
Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu
        1475                1480                1485            
Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe
    1490                1495                1500                
Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp
1505                1510                1515               1520 
Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Tyr
                1525                1530                1535    
Ile Asp Arg Ser Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys
            1540                1545                1550        
Gly Asp Val Glu Glu Asn Pro Gly Pro Arg Leu Glu Met Thr Glu Tyr
        1555                1560                1565            
Lys Pro Thr Val Arg Leu Ala Thr Arg Asp Asp Val Pro Arg Ala Val
    1570                1575                1580                
Arg Thr Leu Ala Ala Ala Phe Ala Asp Tyr Pro Ala Thr Arg His Thr
1585                1590                1595               1600 
Val Asp Pro Asp Arg His Ile Glu Arg Val Thr Glu Leu Gln Glu Leu
                1605                1610                1615    
Phe Leu Thr Arg Val Gly Leu Asp Ile Gly Lys Val Trp Val Ala Asp
            1620                1625                1630        
Asp Gly Ala Ala Val Ala Val Trp Thr Thr Pro Glu Ser Val Glu Ala
        1635                1640                1645            
Gly Ala Val Phe Ala Glu Ile Gly Pro Arg Met Ala Glu Leu Ser Gly
    1650                1655                1660                
Ser Arg Leu Ala Ala Gln Gln Gln Met Glu Gly Leu Leu Ala Pro His
1665                1670                1675               1680 
Arg Pro Lys Glu Pro Ala Trp Phe Leu Ala Thr Val Gly Val Ser Pro
                1685                1690                1695    
Asp His Gln Gly Lys Gly Leu Gly Ser Ala Val Val Leu Pro Gly Val
            1700                1705                1710        
Glu Ala Ala Glu Arg Ala Gly Val Pro Ala Phe Leu Glu Thr Ser Ala
        1715                1720                1725            
Pro Arg Asn Leu Pro Phe Tyr Glu Arg Leu Gly Phe Thr Val Thr Ala
    1730                1735                1740                
Asp Val Glu Val Pro Glu Gly Pro Arg Thr Trp Cys Met Thr Arg Lys
1745                1750                1755               1760 
Pro Gly Ala
            


<210> 479
<211> 1833
<212> PRT
<213> Unknown

<220> 
<223> dCas9VP160-2A-neo

<400> 479
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Gly Ser Gly Arg Ala Asp
        1395                1400                1405            
Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp
    1410                1415                1420                
Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp
1425                1430                1435               1440 
Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met
                1445                1450                1455    
Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser
            1460                1465                1470        
Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu
        1475                1480                1485            
Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe
    1490                1495                1500                
Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp
1505                1510                1515               1520 
Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Tyr
                1525                1530                1535    
Ile Asp Arg Ser Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys
            1540                1545                1550        
Gly Asp Val Glu Glu Asn Pro Gly Pro Arg Leu Glu Thr Arg Met Gly
        1555                1560                1565            
Ser Ala Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp
    1570                1575                1580                
Val Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys
1585                1590                1595               1600 
Ser Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu
                1605                1610                1615    
Phe Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu
            1620                1625                1630        
Ala Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala
        1635                1640                1645            
Val Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly
    1650                1655                1660                
Glu Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu
1665                1670                1675               1680 
Lys Val Ser Ile Met Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp
                1685                1690                1695    
Pro Ala Thr Cys Pro Phe Asp His Gln Ala Lys His Arg Ile Glu Arg
            1700                1705                1710        
Ala Arg Thr Arg Met Glu Ala Gly Leu Val Asp Gln Asp Asp Leu Asp
        1715                1720                1725            
Glu Glu His Gln Gly Leu Ala Pro Ala Glu Leu Phe Ala Arg Leu Lys
    1730                1735                1740                
Ala Arg Met Pro Asp Gly Glu Asp Leu Val Val Thr His Gly Asp Ala
1745                1750                1755               1760 
Cys Leu Pro Asn Ile Met Val Glu Asn Gly Arg Phe Ser Gly Phe Ile
                1765                1770                1775    
Asp Cys Gly Arg Leu Gly Val Ala Asp Arg Tyr Gln Asp Ile Ala Leu
            1780                1785                1790        
Ala Thr Arg Asp Ile Ala Glu Glu Leu Gly Gly Glu Trp Ala Asp Arg
        1795                1800                1805            
Phe Leu Val Leu Tyr Gly Ile Ala Ala Pro Asp Ser Gln Arg Ile Ala
    1810                1815                1820                
Phe Tyr Arg Leu Leu Asp Glu Phe Phe
1825                1830            


<210> 480
<211> 1674
<212> PRT
<213> Unknown

<220> 
<223> dCas9p65

<400> 480
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Ser Pro Met Glu Phe Gln
        1395                1400                1405            
Tyr Leu Pro Asp Thr Asp Asp Arg His Arg Ile Glu Glu Lys Arg Lys
    1410                1415                1420                
Arg Thr Tyr Glu Thr Phe Lys Ser Ile Met Lys Lys Ser Pro Phe Ser
1425                1430                1435               1440 
Gly Pro Thr Asp Pro Arg Pro Pro Pro Arg Arg Ile Ala Val Pro Ser
                1445                1450                1455    
Arg Ser Ser Ala Ser Val Pro Lys Pro Ala Pro Gln Pro Tyr Pro Phe
            1460                1465                1470        
Thr Ser Ser Leu Ser Thr Ile Asn Tyr Asp Glu Phe Pro Thr Met Val
        1475                1480                1485            
Phe Pro Ser Gly Gln Ile Ser Gln Ala Ser Ala Leu Ala Pro Ala Pro
    1490                1495                1500                
Pro Gln Val Leu Pro Gln Ala Pro Ala Pro Ala Pro Ala Pro Ala Met
1505                1510                1515               1520 
Val Ser Ala Leu Ala Gln Ala Pro Ala Pro Val Pro Val Leu Ala Pro
                1525                1530                1535    
Gly Pro Pro Gln Ala Val Ala Pro Pro Ala Pro Lys Pro Thr Gln Ala
            1540                1545                1550        
Gly Glu Gly Thr Leu Ser Glu Ala Leu Leu Gln Leu Gln Phe Asp Asp
        1555                1560                1565            
Glu Asp Leu Gly Ala Leu Leu Gly Asn Ser Thr Asp Pro Ala Val Phe
    1570                1575                1580                
Thr Asp Leu Ala Ser Val Asp Asn Ser Glu Phe Gln Gln Leu Leu Asn
1585                1590                1595               1600 
Gln Gly Ile Pro Val Ala Pro His Thr Thr Glu Pro Met Leu Met Glu
                1605                1610                1615    
Tyr Pro Glu Ala Ile Thr Arg Leu Val Thr Gly Ala Gln Arg Pro Pro
            1620                1625                1630        
Asp Pro Ala Pro Ala Pro Leu Gly Ala Pro Gly Leu Pro Asn Gly Leu
        1635                1640                1645            
Leu Ser Gly Asp Glu Asp Phe Ser Ser Ile Ala Asp Met Asp Phe Ser
    1650                1655                1660                
Ala Leu Leu Ser Gln Ile Ser Ser Ile Asp
1665                1670                


<210> 481
<211> 1495
<212> PRT
<213> Unknown

<220> 
<223> dCas9KRAB

<400> 481
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Ala Ser Pro Lys Lys Lys
        1395                1400                1405            
Arg Lys Val Glu Ala Ser Met Asp Ala Lys Ser Leu Thr Ala Trp Ser
    1410                1415                1420                
Arg Thr Leu Val Thr Phe Lys Asp Val Phe Val Asp Phe Thr Arg Glu
1425                1430                1435               1440 
Glu Trp Lys Leu Leu Asp Thr Ala Gln Gln Ile Val Tyr Arg Asn Val
                1445                1450                1455    
Met Leu Glu Asn Tyr Lys Asn Leu Val Ser Leu Gly Tyr Gln Leu Thr
            1460                1465                1470        
Lys Pro Asp Val Ile Leu Arg Leu Glu Lys Gly Glu Glu Pro Trp Leu
        1475                1480                1485            
Val Ser Arg Gly Ser Ile Asp
    1490                1495


<210> 482
<211> 1532
<212> PRT
<213> Unknown

<220> 
<223> dCas9PCP

<400> 482
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Ile Asp Met Ser Lys Thr
        1395                1400                1405            
Ile Val Leu Ser Val Gly Glu Ala Thr Arg Thr Leu Thr Glu Ile Gln
    1410                1415                1420                
Ser Thr Ala Asp Arg Gln Ile Phe Glu Glu Lys Val Gly Pro Leu Val
1425                1430                1435               1440 
Gly Arg Leu Arg Leu Thr Ala Ser Leu Arg Gln Asn Gly Ala Lys Thr
                1445                1450                1455    
Ala Tyr Arg Val Asn Leu Lys Leu Asp Gln Ala Asp Val Val Asp Cys
            1460                1465                1470        
Ser Thr Ser Val Cys Gly Glu Leu Pro Lys Val Arg Tyr Thr Gln Val
        1475                1480                1485            
Trp Ser His Asp Val Thr Ile Val Ala Asn Ser Thr Glu Ala Ser Arg
    1490                1495                1500                
Lys Ser Leu Tyr Asp Leu Thr Lys Ser Leu Val Ala Thr Ser Gln Val
1505                1510                1515               1520 
Glu Asp Leu Val Val Asn Leu Val Pro Leu Gly Arg
                1525                1530        


<210> 483
<211> 1520
<212> PRT
<213> Unknown

<220> 
<223> dCas9MS2

<400> 483
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Met Ala Ser Asn Phe Thr
        1395                1400                1405            
Gln Phe Val Leu Val Asp Asn Gly Gly Thr Gly Asp Val Thr Val Ala
    1410                1415                1420                
Pro Ser Asn Phe Ala Asn Gly Ile Ala Glu Trp Ile Ser Ser Asn Ser
1425                1430                1435               1440 
Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser Val Arg Gln Ser Ser Ala
                1445                1450                1455    
Gln Asn Arg Lys Tyr Thr Ile Lys Val Glu Val Pro Lys Gly Ala Trp
            1460                1465                1470        
Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe Ala Thr Asn
        1475                1480                1485            
Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu Leu Lys Asp
    1490                1495                1500                
Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly Ile Tyr Ala
1505                1510                1515               1520 


<210> 484
<211> 1852
<212> PRT
<213> Unknown

<220> 
<223> dCas9VP160ER

<400> 484
Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Ser Pro Lys Lys Lys Arg
 1               5                  10                  15      
Lys Val Glu Ala Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
            20                  25                  30          
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
        35                  40                  45              
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
    50                  55                  60                  
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
65                  70                  75                  80  
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
                85                  90                  95      
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
            100                 105                 110         
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
        115                 120                 125             
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
    130                 135                 140                 
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
145                 150                 155                 160 
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
                165                 170                 175     
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
            180                 185                 190         
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
        195                 200                 205             
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
    210                 215                 220                 
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
225                 230                 235                 240 
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
                245                 250                 255     
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
            260                 265                 270         
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
        275                 280                 285             
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
    290                 295                 300                 
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
305                 310                 315                 320 
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
                325                 330                 335     
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
            340                 345                 350         
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
        355                 360                 365             
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
    370                 375                 380                 
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
385                 390                 395                 400 
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
                405                 410                 415     
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His
            420                 425                 430         
Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
        435                 440                 445             
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu
    450                 455                 460                 
Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser
465                 470                 475                 480 
Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp
                485                 490                 495     
Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile
            500                 505                 510         
Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu
        515                 520                 525             
Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu
    530                 535                 540                 
Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu
545                 550                 555                 560 
Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn
                565                 570                 575     
Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile
            580                 585                 590         
Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn
        595                 600                 605             
Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys
    610                 615                 620                 
Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val
625                 630                 635                 640 
Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
                645                 650                 655     
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys
            660                 665                 670         
Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
        675                 680                 685             
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys
    690                 695                 700                 
Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp
705                 710                 715                 720 
Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln
                725                 730                 735     
Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
            740                 745                 750         
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val
        755                 760                 765             
Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala
    770                 775                 780                 
Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg
785                 790                 795                 800 
Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu
                805                 810                 815     
Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr
            820                 825                 830         
Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu
        835                 840                 845             
Asp Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln
    850                 855                 860                 
Ser Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser
865                 870                 875                 880 
Asp Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
                885                 890                 895     
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile
            900                 905                 910         
Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
        915                 920                 925             
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr
    930                 935                 940                 
Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn
945                 950                 955                 960 
Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile
                965                 970                 975     
Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
            980                 985                 990         
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr
        995                 1000                1005            
Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu
    1010                1015                1020                
Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys
1025                1030                1035               1040 
Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr
                1045                1050                1055    
Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
            1060                1065                1070        
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu
        1075                1080                1085            
Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg
    1090                1095                1100                
Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val
1105                1110                1115               1120 
Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
                1125                1130                1135    
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly
            1140                1145                1150        
Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
        1155                1160                1165            
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu
    1170                1175                1180                
Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp
1185                1190                1195               1200 
Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile
                1205                1210                1215    
Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
            1220                1225                1230        
Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu
        1235                1240                1245            
Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys
    1250                1255                1260                
Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu
1265                1270                1275               1280 
Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe
                1285                1290                1295    
Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
            1300                1305                1310        
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn
        1315                1320                1325            
Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe
    1330                1335                1340                
Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys
1345                1350                1355               1360 
Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Pro Lys Lys
            1380                1385                1390        
Lys Arg Lys Val Glu Ala Ser Gly Pro Ala Gly Ser Gly Arg Ala Asp
        1395                1400                1405            
Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp
    1410                1415                1420                
Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp
1425                1430                1435               1440 
Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met
                1445                1450                1455    
Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser
            1460                1465                1470        
Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu
        1475                1480                1485            
Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe
    1490                1495                1500                
Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp
1505                1510                1515               1520 
Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Tyr
                1525                1530                1535    
Ile Asp Ser Ala Gly Asp Met Arg Ala Ala Asn Leu Trp Pro Ser Pro
            1540                1545                1550        
Leu Met Ile Lys Arg Ser Lys Lys Asn Ser Leu Ala Leu Ser Leu Thr
        1555                1560                1565            
Ala Asp Gln Met Val Ser Ala Leu Leu Asp Ala Glu Pro Pro Ile Leu
    1570                1575                1580                
Tyr Ser Glu Tyr Asp Pro Thr Arg Pro Phe Ser Glu Ala Ser Met Met
1585                1590                1595               1600 
Gly Leu Leu Thr Asn Leu Ala Asp Arg Glu Leu Val His Met Ile Asn
                1605                1610                1615    
Trp Ala Lys Arg Val Pro Gly Phe Val Asp Leu Thr Leu His Asp Gln
            1620                1625                1630        
Val His Leu Leu Glu Cys Ala Trp Leu Glu Ile Leu Met Ile Gly Leu
        1635                1640                1645            
Val Trp Arg Ser Met Glu His Pro Val Lys Leu Leu Phe Ala Pro Asn
    1650                1655                1660                
Leu Leu Leu Asp Arg Asn Gln Gly Lys Cys Val Glu Gly Met Val Glu
1665                1670                1675               1680 
Ile Phe Asp Met Leu Leu Ala Thr Ser Ser Arg Phe Arg Met Met Asn
                1685                1690                1695    
Leu Gln Gly Glu Glu Phe Val Cys Leu Lys Ser Ile Ile Leu Leu Asn
            1700                1705                1710        
Ser Gly Val Tyr Thr Phe Leu Ser Ser Thr Leu Lys Ser Leu Glu Glu
        1715                1720                1725            
Lys Asp His Ile His Arg Val Leu Asp Lys Ile Thr Asp Thr Leu Ile
    1730                1735                1740                
His Leu Met Ala Lys Ala Gly Leu Thr Leu Gln Gln Gln His Gln Arg
1745                1750                1755               1760 
Leu Ala Gln Leu Leu Leu Ile Leu Ser His Ile Arg His Met Ser Asn
                1765                1770                1775    
Lys Gly Met Glu His Leu Tyr Ser Met Lys Cys Lys Asn Val Val Pro
            1780                1785                1790        
Leu Tyr Asp Leu Leu Leu Glu Ala Ala Asp Ala His Arg Leu His Ala
        1795                1800                1805            
Pro Thr Ser Arg Gly Gly Ala Ser Val Glu Glu Thr Asp Gln Ser His
    1810                1815                1820                
Leu Ala Thr Ala Gly Ser Thr Ser Ser His Ser Leu Gln Lys Tyr Tyr
1825                1830                1835               1840 
Ile Thr Gly Glu Ala Glu Gly Phe Pro Ala Thr Val
                1845                1850        


<210> 485
<211> 4107
<212> DNA
<213> Unknown

<220> 
<223> wt Cas9 (s. Pyogenes)

<400> 485
atggataaga aatactcaat aggcttagat atcggcacaa atagcgtcgg atgggcggtg 60
atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc 120
cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa 180
gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt 240
tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga 300
cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga 360
aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa 420
aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat 480
atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat 540
gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct 600
attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga 660
cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat 720
ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa 780
gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg 840
caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt 900
ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca 960
atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga 1020
caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca 1080
ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta 1140
gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc 1200
aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat 1260
gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt 1320
gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg tggcaatagt 1380
cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa 1440
gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa 1500
aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt 1560
tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt 1620
tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc 1680
gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt 1740
tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt 1800
attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt 1860
ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct 1920
cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga 1980
cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta 2040
gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat 2100
agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta 2160
catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact 2220
gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt 2280
attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt 2340
atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct 2400
gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga 2460
gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatcac 2520
attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct 2580
gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa 2640
aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta 2700
acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa 2760
ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat 2820
actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct 2880
aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat 2940
taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa 3000
tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa 3060
atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct 3120
aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc 3180
cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt 3240
gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta 3300
cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt 3360
gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct 3420
tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt 3480
aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac 3540
tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa 3600
tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta 3660
caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt 3720
cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag 3780
cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt 3840
attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa 3900
ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct 3960
cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa 4020
gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt 4080
gatttgagtc agctaggagg tgactga                                     4107

<210> 486
<211> 7372
<212> DNA
<213> Unknown

<220> 
<223> dCas9VP64 on pmax expression vector

<400> 486
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60
ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120
aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180
gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240
gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300
agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360
ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420
cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480
gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540
caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600
caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 660
cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg 720
tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat 780
tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt 840
aaggaggcca tagaaactgg gcttgtcgag acagagaaga ttcttgcgtt tctgataggc 900
acctattggt cttactgaca tccactttgc ctttctctcc acagggaaaa aacaattgac 960
aagtttgtac aaaaaagcag gctccgaatt caccggtgcc accatgtacc catacgatgt 1020
tccagattac gcttcgccga agaaaaagcg caaggtcgaa gcgtccgaca agaagtacag 1080
catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa 1140
ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa 1200
cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag 1260
aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt 1320
cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct 1380
ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt 1440
ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac 1500
cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg 1560
ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat 1620
ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt 1680
ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat 1740
cgcccagctg cccggcgaga agaagaatgg cctgttcggc aacctgattg ccctgagcct 1800
gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct 1860
gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta 1920
cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct 1980
gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga 2040
cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa 2100
gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg 2160
agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac 2220
cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga 2280
caacggcagc atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca 2340
ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt 2400
ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac 2460
cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc 2520
ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa 2580
ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa 2640
agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa 2700
ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga 2760
ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg 2820
gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt 2880
cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt 2940
tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa 3000
agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct 3060
gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga 3120
cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga 3180
ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa 3240
tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga 3300
gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga 3360
gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga 3420
gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct 3480
gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca 3540
ggaactggac atcaaccggc tgtccgacta cgatgtggac gccatcgtgc ctcagagctt 3600
tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa 3660
gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct 3720
gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg 3780
cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca 3840
gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa 3900
tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt 3960
ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga 4020
cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag 4080
cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga 4140
gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt 4200
caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa 4260
cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt 4320
gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag 4380
caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg 4440
ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt 4500
ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat 4560
caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg 4620
ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct 4680
ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact 4740
ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa 4800
gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct 4860
ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa 4920
tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc 4980
cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta 5040
ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac 5100
cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg 5160
aggcgacagc cccaagaaga agagaaaggt ggaggccagc gggccggccg gatccgggcg 5220
cgccgacgcg ctggacgatt tcgatctcga catgctgggt tctgatgccc tcgatgactt 5280
tgacctggat atgttgggaa gcgacgcatt ggatgacttt gatctggaca tgctcggctc 5340
cgatgctctg gacgatttcg atctcgatat gttatatatc gattgattaa ttaagaattc 5400
gacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgagtcta 5460
gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg 5520
cgtaccggtt agcaattgtt ttttcgatga gtttggacaa accacaacta gaatgcagtg 5580
aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 5640
ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 5700
ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtactt aagaggggga 5760
gaccaaaggg cgagacgtta aggcctcacg tgacatgtga gcaaaaggcc agcaaaaggc 5820
caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 5880
gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 5940
ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 6000
cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 6060
taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 6120
cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 6180
acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 6240
aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 6300
atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 6360
atccggcaaa caaaccacgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 6420
cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 6480
tggaacgaaa actcacgtta agggattttg gtcatgccgt ctcagaagaa ctcgtcaaga 6540
aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag 6600
cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc 6660
tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt 6720
tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg 6780
ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg 6840
tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga 6900
tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt 6960
gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc 7020
cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca 7080
gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt 7140
tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac 7200
agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat 7260
agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcataata 7320
ttattgaagc atttatcagg gttcgtctcg tcccggtctc ctcccatgca tg         7372



<210> 487
<211> 7451
<212> DNA
<213> Unknown

<220> 
<223> dCas9VP96 on pmax expression vector

<400> 487
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60
ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120
aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180
gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240
gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300
agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360
ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420
cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480
gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540
caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600
caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 660
cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg 720
tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat 780
tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt 840
aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg 900
cacctattgg tcttactgac atccactttg cctttctctc cacagggaaa aaacaattga 960
caagtttgta caaaaaagca ggctccgaat tcaccggtgc caccatgtac ccatacgatg 1020
ttccagatta cgcttcgccg aagaaaaagc gcaaggtcga agcgtccgac aagaagtaca 1080
gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca 1140
aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga 1200
acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga 1260
gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct 1320
tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc 1380
tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg 1440
tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca 1500
ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg 1560
gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca 1620
tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg 1680
tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga 1740
tcgcccagct gcccggcgag aagaagaatg gcctgttcgg caacctgatt gccctgagcc 1800
tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc 1860
tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt 1920
acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc 1980
tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg 2040
acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga 2100
agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg 2160
gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca 2220
ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg 2280
acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc 2340
aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct 2400
tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga 2460
ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg 2520
cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga 2580
aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca 2640
aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa 2700
aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag 2760
aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc 2820
ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact 2880
tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt 2940
ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca 3000
aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc 3060
tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg 3120
acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag 3180
aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca 3240
atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg 3300
agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag 3360
agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag 3420
agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc 3480
tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc 3540
aggaactgga catcaaccgg ctgtccgact acgatgtgga cgccatcgtg cctcagagct 3600
ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca 3660
agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc 3720
tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag 3780
gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc 3840
agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga 3900
atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt 3960
tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg 4020
acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa 4080
gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg 4140
agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt 4200
tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa 4260
acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag 4320
tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca 4380
gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact 4440
gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg 4500
tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga 4560
tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg 4620
gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc 4680
tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac 4740
tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga 4800
agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc 4860
tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta 4920
atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg 4980
ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt 5040
actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca 5100
ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg 5160
gaggcgacag ccccaagaag aagagaaagg tggaggccag cgggccggcc ggatccgggc 5220
gcgccgacgc gctggacgat ttcgatctcg acatgctggg ttctgatgcc ctcgatgact 5280
ttgacctgga tatgttggga agcgacgcat tggatgactt tgatctggac atgctcggct 5340
ccgatgctct ggacgatttc gatctcgata tgttagggtc agacgcactg gatgatttcg 5400
accttgatat gttgggaagc gatgcccttg atgatttcga tttggatatg ttgtatatcg 5460
attgattaat taagaattcg acccagcttt cttgtacaaa gtggttgata tccagcacag 5520
tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct 5580
cctcggtctc gattctacgc gtaccggtta gcaattgttt tttcgatgag tttggacaaa 5640
ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt 5700
tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta 5760
tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat 5820
gtggtactta agagggggag accaaagggc gagacgttaa ggcctcacgt gacatgtgag 5880
caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 5940
ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 6000
cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 6060
ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 6120
tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 6180
gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 6240
ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 6300
ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 6360
gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 6420
aaagagttgg tagctcttga tccggcaaac aaaccacgct ggtagcggtg gtttttttgt 6480
ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 6540
tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgccgtc 6600
tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat 6660
accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg 6720
ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa 6780
tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac 6840
gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc 6900
gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt 6960
acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag 7020
cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg 7080
agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc 7140
agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg 7200
cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac 7260
cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg 7320
tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc 7380
atcttgttca atcataatat tattgaagca tttatcaggg ttcgtctcgt cccggtctcc 7440
tcccatgcat g                                                      7451



<210> 488
<211> 7607
<212> DNA
<213> Unknown

<220> 
<223> dCas9VP160 on pmax expression vector

<400> 488
tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60
ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120
aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180
gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 240
gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300
agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360
ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420
cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480
gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540
caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600
caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 660
cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg 720
tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat 780
tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt 840
aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg 900
cacctattgg tcttactgac atccactttg cctttctctc cacagggaaa aaacaattga 960
caagtttgta caaaaaagca ggctccgaat tcaccggtgc caccatgtac ccatacgatg 1020
ttccagatta cgcttcgccg aagaaaaagc gcaaggtcga agcgtccgac aagaagtaca 1080
gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca 1140
aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga 1200
acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga 1260
gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct 1320
tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc 1380
tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg 1440
tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca 1500
ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg 1560
gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca 1620
tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg 1680
tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga 1740
tcgcccagct gcccggcgag aagaagaatg gcctgttcgg caacctgatt gccctgagcc 1800
tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc 1860
tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt 1920
acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc 1980
tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg 2040
acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga 2100
agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg 2160
gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca 2220
ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg 2280
acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc 2340
aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct 2400
tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga 2460
ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg 2520
cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga 2580
aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca 2640
aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa 2700
aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag 2760
aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc 2820
ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact 2880
tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt 2940
ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca 3000
aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc 3060
tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg 3120
acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag 3180
aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca 3240
atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg 3300
agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag 3360
agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag 3420
agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc 3480
tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc 3540
aggaactgga catcaaccgg ctgtccgact acgatgtgga cgccatcgtg cctcagagct 3600
ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca 3660
agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc 3720
tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag 3780
gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc 3840
agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga 3900
atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt 3960
tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg 4020
acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa 4080
gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg 4140
agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt 4200
tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa 4260
acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag 4320
tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca 4380
gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact 4440
gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg 4500
tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga 4560
tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg 4620
gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc 4680
tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac 4740
tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga 4800
agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc 4860
tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta 4920
atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg 4980
ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt 5040
actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca 5100
ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg 5160
gaggcgacag ccccaagaag aagagaaagg tggaggccag cgggccggcc ggatccgggc 5220
gcgccgacgc gctggacgat ttcgatctcg acatgctggg ttctgatgcc ctcgatgact 5280
ttgacctgga tatgttggga agcgacgcat tggatgactt tgatctggac atgctcggct 5340
ccgatgctct ggacgatttc gatctcgata tgttagggtc agacgcactg gatgatttcg 5400
accttgatat gttgggaagc gatgcccttg atgatttcga cctggacatg ctcggcagcg 5460
acgccctgga cgatttcgat ctggacatgc tggggtccga tgccttggat gattttgact 5520
tggatatgct ggggagtgat gccctggacg actttgacct ggacatgctg ggctccgatg 5580
cgctcgatga cttcgatttg gatatgttgt atatcgattg attaattaag aattcgaccc 5640
agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg 5700
cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac 5760
cggttagcaa ttgttttttc gatgagtttg gacaaaccac aactagaatg cagtgaaaaa 5820
aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca 5880
ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt 5940
gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tacttaagag ggggagacca 6000
aagggcgaga cgttaaggcc tcacgtgaca tgtgagcaaa aggccagcaa aaggccagga 6060
accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 6120
acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 6180
cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 6240
acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 6300
atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 6360
agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 6420
acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 6480
gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 6540
gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 6600
gcaaacaaac cacgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 6660
aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6720
cgaaaactca cgttaaggga ttttggtcat gccgtctcag aagaactcgt caagaaggcg 6780
atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc 6840
agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata 6900
gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac 6960
catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat 7020
gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag 7080
atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt 7140
cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc 7200
agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg 7260
cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc 7320
gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt 7380
cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg 7440
gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct 7500
ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca taatattatt 7560
gaagcattta tcagggttcg tctcgtcccg gtctcctccc atgcatg               7607


