                         SEQUENCE LISTING

<110>  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
       DELMAS, Bernard
 
<120>  MUTANTS THERMOSENSIBLES DE VIRUS INFLUENZA

<130>  F539-160-PCT-HLP-ll

<150>  FR1353543
<151>  2013-04-18

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  716
<212>  PRT
<213>  Influenza A virus


<220>
<221>  domaine N-terminal
<222>  (1)..(196)

<220>
<221>  charnire
<222>  (197)..(257)

<220>
<221>  domaine C-terminal
<222>  (258)..(716)

<400>  1

Met Glu Asp Phe Val Arg Gln Cys Phe Asn Pro Met Ile Val Glu Leu 
1               5                   10                  15      


Ala Glu Lys Ala Met Lys Glu Tyr Gly Glu Asp Leu Lys Ile Glu Thr 
            20                  25                  30          


Asn Lys Phe Ala Ala Ile Cys Thr His Leu Glu Val Cys Phe Met Tyr 
        35                  40                  45              


Ser Asp Phe His Phe Ile Asp Glu Gln Gly Glu Ser Ile Val Val Glu 
    50                  55                  60                  


Leu Gly Asp Pro Asn Ala Leu Leu Lys His Arg Phe Glu Ile Ile Glu 
65                  70                  75                  80  


Gly Arg Asp Arg Thr Ile Ala Trp Thr Val Ile Asn Ser Ile Cys Asn 
                85                  90                  95      


Thr Thr Gly Ala Glu Lys Pro Lys Phe Leu Pro Asp Leu Tyr Asp Tyr 
            100                 105                 110         


Lys Lys Asn Arg Phe Ile Glu Ile Gly Val Thr Arg Arg Glu Val His 
        115                 120                 125             


Ile Tyr Tyr Leu Glu Lys Ala Asn Lys Ile Lys Ser Glu Lys Thr His 
    130                 135                 140                 


Ile His Ile Phe Ser Phe Thr Gly Glu Glu Met Ala Thr Lys Ala Asp 
145                 150                 155                 160 


Tyr Thr Leu Asp Glu Glu Ser Arg Ala Arg Ile Lys Thr Arg Leu Phe 
                165                 170                 175     


Thr Ile Arg Gln Glu Met Ala Ser Arg Gly Leu Trp Asp Ser Phe Arg 
            180                 185                 190         


Gln Ser Glu Arg Gly Glu Glu Thr Ile Glu Glu Arg Phe Glu Ile Thr 
        195                 200                 205             


Gly Thr Met Arg Lys Leu Ala Asp Gln Ser Leu Pro Pro Asn Phe Ser 
    210                 215                 220                 


Ser Leu Glu Asn Phe Arg Ala Tyr Val Asp Gly Phe Glu Pro Asn Gly 
225                 230                 235                 240 


Tyr Ile Glu Gly Lys Leu Ser Gln Met Ser Lys Glu Val Asn Ala Arg 
                245                 250                 255     


Ile Glu Pro Phe Leu Lys Ser Thr Pro Arg Pro Leu Arg Leu Pro Asp 
            260                 265                 270         


Gly Pro Pro Cys Ser Gln Arg Ser Lys Phe Leu Leu Met Asp Ala Leu 
        275                 280                 285             


Lys Leu Ser Ile Glu Asp Pro Ser His Glu Gly Glu Gly Ile Pro Leu 
    290                 295                 300                 


Tyr Asp Ala Ile Lys Cys Met Arg Thr Phe Phe Gly Trp Lys Glu Pro 
305                 310                 315                 320 


Asn Val Val Lys Pro His Glu Lys Gly Ile Asn Pro Asn Tyr Leu Leu 
                325                 330                 335     


Ser Trp Lys Gln Val Leu Ala Glu Leu Gln Asp Ile Glu Asn Glu Glu 
            340                 345                 350         


Lys Ile Pro Arg Thr Lys Asn Met Lys Lys Thr Ser Gln Leu Lys Trp 
        355                 360                 365             


Ala Leu Gly Glu Asn Met Ala Pro Glu Lys Val Asp Phe Asp Asp Cys 
    370                 375                 380                 


Lys Asp Val Gly Asp Leu Lys Gln Tyr Asp Ser Asp Glu Pro Glu Leu 
385                 390                 395                 400 


Arg Ser Leu Ala Ser Trp Ile Gln Asn Glu Phe Asn Lys Ala Cys Glu 
                405                 410                 415     


Leu Thr Asp Ser Ser Trp Ile Glu Leu Asp Glu Ile Gly Glu Asp Ala 
            420                 425                 430         


Ala Pro Ile Glu His Ile Ala Ser Met Arg Arg Asn Tyr Phe Thr Ala 
        435                 440                 445             


Glu Val Ser His Cys Arg Ala Thr Glu Tyr Ile Met Lys Gly Val Tyr 
    450                 455                 460                 


Ile Asn Thr Ala Leu Leu Asn Ala Ser Cys Ala Ala Met Asp Asp Phe 
465                 470                 475                 480 


Gln Leu Ile Pro Met Ile Ser Lys Cys Arg Thr Lys Glu Gly Arg Arg 
                485                 490                 495     


Lys Thr Asn Leu Tyr Gly Phe Ile Ile Lys Gly Arg Ser His Leu Arg 
            500                 505                 510         


Asn Asp Thr Asp Val Val Asn Phe Val Ser Met Glu Phe Ser Leu Thr 
        515                 520                 525             


Asp Pro Arg Leu Glu Pro His Lys Trp Glu Lys Tyr Cys Val Leu Glu 
    530                 535                 540                 


Val Gly Asp Met Leu Leu Arg Ser Ala Ile Gly His Val Ser Arg Pro 
545                 550                 555                 560 


Met Phe Leu Tyr Val Arg Thr Asn Gly Thr Ser Lys Ile Lys Met Lys 
                565                 570                 575     


Trp Gly Met Glu Met Arg Arg Cys Leu Leu Gln Ser Leu Gln Gln Ile 
            580                 585                 590         


Glu Ser Met Ile Glu Ala Glu Ser Ser Val Lys Glu Lys Asp Met Thr 
        595                 600                 605             


Lys Glu Phe Phe Glu Asn Lys Ser Glu Thr Trp Pro Val Gly Glu Ser 
    610                 615                 620                 


Pro Lys Gly Val Glu Glu Gly Ser Ile Gly Lys Val Cys Arg Thr Leu 
625                 630                 635                 640 


Leu Ala Lys Ser Val Phe Asn Ser Leu Tyr Ala Ser Pro Gln Leu Glu 
                645                 650                 655     


Gly Phe Ser Ala Glu Ser Arg Lys Leu Leu Leu Ile Val Gln Ala Leu 
            660                 665                 670         


Arg Asp Asn Leu Glu Pro Gly Thr Phe Asp Leu Gly Gly Leu Tyr Glu 
        675                 680                 685             


Ala Ile Glu Glu Cys Leu Ile Asn Asp Pro Trp Val Leu Leu Asn Ala 
    690                 695                 700                 


Ser Trp Phe Asn Ser Phe Leu Thr His Ala Leu Arg 
705                 710                 715     


<210>  2
<211>  726
<212>  PRT
<213>  Influenza B virus


<220>
<221>  domaine N-terminal
<222>  (1)..(195)

<220>
<221>  charnire
<222>  (196)..(254)

<220>
<221>  domaine C-terminal
<222>  (255)..(726)

<400>  2

Met Asp Thr Phe Ile Thr Arg Asn Phe Gln Thr Thr Ile Ile Gln Lys 
1               5                   10                  15      


Ala Lys Asn Thr Met Ala Glu Phe Ser Glu Asp Pro Glu Leu Gln Pro 
            20                  25                  30          


Ala Met Leu Phe Asn Ile Cys Val His Leu Glu Val Cys Tyr Val Ile 
        35                  40                  45              


Ser Asp Met Asn Phe Leu Asp Glu Glu Gly Lys Thr Tyr Thr Ala Leu 
    50                  55                  60                  


Glu Gly Gln Gly Lys Glu Gln Asn Leu Arg Pro Gln Tyr Glu Val Ile 
65                  70                  75                  80  


Glu Gly Met Pro Arg Asn Ile Ala Trp Met Val Gln Arg Ser Leu Ala 
                85                  90                  95      


Gln Glu His Gly Ile Glu Thr Pro Arg Tyr Leu Ala Asp Leu Phe Asp 
            100                 105                 110         


Tyr Lys Thr Lys Arg Phe Ile Glu Val Gly Ile Thr Lys Gly Leu Ala 
        115                 120                 125             


Asp Asp Tyr Phe Trp Lys Lys Lys Glu Lys Leu Gly Asn Ser Met Glu 
    130                 135                 140                 


Leu Met Ile Phe Ser Tyr Asn Gln Asp Tyr Ser Leu Ser Asn Glu Ser 
145                 150                 155                 160 


Ser Leu Asp Glu Glu Gly Lys Gly Arg Val Leu Ser Arg Leu Thr Glu 
                165                 170                 175     


Leu Gln Ala Glu Leu Ser Leu Lys Asn Leu Trp Gln Val Leu Ile Gly 
            180                 185                 190         


Glu Glu Asp Ile Glu Lys Gly Ile Asp Phe Lys Leu Gly Gln Thr Ile 
        195                 200                 205             


Ser Lys Leu Arg Asp Ile Ser Val Pro Ala Gly Phe Ser Asn Phe Glu 
    210                 215                 220                 


Gly Met Arg Ser Tyr Ile Asp Asn Ile Asp Pro Lys Gly Ala Ile Glu 
225                 230                 235                 240 


Arg Asn Leu Ala Arg Met Ser Pro Leu Val Ser Val Thr Pro Lys Lys 
                245                 250                 255     


Leu Lys Trp Glu Asp Leu Arg Pro Ile Gly Pro His Ile Tyr Asn His 
            260                 265                 270         


Glu Leu Pro Glu Val Pro Tyr Asn Ala Phe Leu Leu Met Ser Asp Glu 
        275                 280                 285             


Leu Gly Leu Ala Asn Met Thr Glu Gly Lys Ser Lys Lys Pro Lys Ile 
    290                 295                 300                 


Leu Ala Lys Glu Cys Leu Glu Lys Tyr Ser Thr Leu Arg Asp Gln Thr 
305                 310                 315                 320 


Asp Pro Ile Leu Ile Met Lys Ser Glu Lys Ala Asn Glu Asn Phe Leu 
                325                 330                 335     


Trp Lys Leu Trp Arg Asp Cys Val Asn Thr Ile Ser Asn Glu Glu Thr 
            340                 345                 350         


Ser Asn Glu Leu Gln Lys Thr Asn Tyr Ala Lys Trp Ala Thr Gly Asp 
        355                 360                 365             


Gly Leu Thr Tyr Gln Lys Ile Met Lys Glu Val Ala Ile Asp Asp Glu 
    370                 375                 380                 


Thr Met Tyr Gln Glu Glu Pro Lys Ile Pro Asn Lys Cys Arg Val Ala 
385                 390                 395                 400 


Ala Trp Val Gln Thr Glu Met Asn Leu Leu Ser Thr Leu Thr Ser Lys 
                405                 410                 415     


Arg Ala Leu Asp Leu Pro Glu Ile Gly Pro Asp Val Ala Pro Val Glu 
            420                 425                 430         


His Val Gly Ser Glu Arg Arg Lys Tyr Phe Val Asn Glu Ile Asn Tyr 
        435                 440                 445             


Cys Lys Ala Ser Thr Val Met Met Lys Tyr Val Leu Phe His Thr Ser 
    450                 455                 460                 


Leu Leu Asn Glu Ser Asn Ala Ser Met Gly Lys Tyr Lys Val Ile Pro 
465                 470                 475                 480 


Ile Thr Asn Arg Val Val Asn Glu Lys Gly Glu Ser Phe Asp Met Leu 
                485                 490                 495     


Tyr Gly Leu Ala Val Lys Gly Gln Ser His Leu Arg Gly Asp Thr Asp 
            500                 505                 510         


Val Val Thr Val Val Thr Phe Glu Phe Ser Ser Thr Asp Pro Arg Val 
        515                 520                 525             


Asp Ser Gly Lys Trp Pro Lys Tyr Thr Val Phe Arg Ile Gly Ser Leu 
    530                 535                 540                 


Phe Val Ser Gly Arg Glu Lys Ser Val Tyr Leu Tyr Cys Arg Val Asn 
545                 550                 555                 560 


Gly Thr Asn Lys Ile Gln Met Lys Trp Gly Met Glu Ala Arg Arg Cys 
                565                 570                 575     


Leu Leu Gln Ser Met Gln Gln Met Glu Ala Ile Val Glu Gln Glu Ser 
            580                 585                 590         


Ser Ile Gln Gly Tyr Asp Met Thr Lys Ala Cys Phe Lys Gly Asp Arg 
        595                 600                 605             


Val Asn Ser Pro Lys Thr Phe Ser Ile Gly Thr Gln Glu Gly Lys Leu 
    610                 615                 620                 


Val Lys Gly Ser Phe Gly Lys Ala Leu Arg Val Ile Phe Thr Lys Cys 
625                 630                 635                 640 


Leu Met His Tyr Val Phe Gly Asn Ala Gln Leu Glu Gly Phe Ser Ala 
                645                 650                 655     


Glu Ser Arg Arg Leu Leu Leu Leu Ile Gln Ala Leu Lys Asp Arg Lys 
            660                 665                 670         


Gly Pro Trp Val Phe Asp Leu Glu Gly Met Tyr Ser Gly Ile Glu Glu 
        675                 680                 685             


Cys Ile Ser Asn Asn Pro Trp Val Ile Gln Ser Ala Tyr Trp Phe Asn 
    690                 695                 700                 


Glu Trp Leu Gly Phe Glu Lys Glu Gly Ser Lys Val Leu Glu Ser Val 
705                 710                 715                 720 


Asp Glu Ile Met Asp Glu 
                725     


<210>  3
<211>  709
<212>  PRT
<213>  Influenza C virus


<220>
<221>  domaine N-terminal
<222>  (1)..(178)

<220>
<221>  charnire
<222>  (179)..(238)

<220>
<221>  domaine C-terminal
<222>  (239)..(709)

<400>  3

Met Ser Lys Thr Phe Ala Glu Ile Ala Glu Ala Phe Leu Glu Pro Glu 
1               5                   10                  15      


Ala Val Arg Ile Ala Lys Glu Ala Val Glu Glu Tyr Gly Asp His Glu 
            20                  25                  30          


Arg Lys Ile Ile Gln Ile Gly Ile His Phe Gln Val Cys Cys Met Phe 
        35                  40                  45              


Cys Asp Glu Tyr Leu Ser Thr Asn Gly Ser Asp Arg Phe Val Leu Ile 
    50                  55                  60                  


Glu Gly Arg Lys Arg Gly Thr Ala Val Ser Leu Gln Asn Glu Leu Cys 
65                  70                  75                  80  


Lys Ser Tyr Asp Leu Glu Pro Leu Pro Phe Leu Cys Asp Ile Phe Asp 
                85                  90                  95      


Arg Glu Glu Lys Gln Phe Val Glu Ile Gly Ile Thr Arg Lys Ala Asp 
            100                 105                 110         


Asp Ser Tyr Phe Gln Ser Lys Phe Gly Lys Leu Gly Asn Ser Cys Lys 
        115                 120                 125             


Ile Phe Val Phe Ser Tyr Asp Gly Arg Leu Asp Lys Asn Cys Glu Gly 
    130                 135                 140                 


Pro Met Glu Glu Gln Lys Leu Arg Ile Phe Ser Phe Leu Ala Thr Ala 
145                 150                 155                 160 


Ala Asp Phe Leu Arg Lys Glu Asn Met Phe Asn Glu Ile Phe Leu Pro 
                165                 170                 175     


Asp Asn Glu Glu Thr Ile Ile Glu Met Lys Lys Gly Lys Thr Phe Leu 
            180                 185                 190         


Lys Leu Arg Asp Glu Ser Val Pro Leu Pro Phe Gln Thr Tyr Glu Gln 
        195                 200                 205             


Met Lys Asp Tyr Cys Glu Lys Phe Lys Gly Asn Pro Arg Glu Leu Ala 
    210                 215                 220                 


Ser Lys Val Ser Gln Met Gln Ser Asn Ile Lys Leu Pro Ile Lys His 
225                 230                 235                 240 


Tyr Glu Gln Asn Lys Phe Arg Gln Ile Arg Leu Pro Lys Gly Pro Met 
                245                 250                 255     


Ala Pro Tyr Thr His Lys Phe Leu Met Glu Glu Ala Trp Met Phe Thr 
            260                 265                 270         


Lys Ile Ser Asp Pro Glu Arg Ser Arg Ala Gly Glu Ile Leu Ile Asp 
        275                 280                 285             


Phe Phe Lys Lys Gly Asn Leu Ser Ala Ile Arg Pro Lys Asp Lys Pro 
    290                 295                 300                 


Leu Gln Gly Lys Tyr Pro Ile His Tyr Lys Asn Leu Trp Asn Gln Ile 
305                 310                 315                 320 


Lys Ala Ala Ile Ala Asp Arg Thr Met Val Ile Asn Glu Asn Asp His 
                325                 330                 335     


Ser Glu Phe Leu Gly Gly Ile Gly Arg Ala Ser Lys Lys Ile Pro Glu 
            340                 345                 350         


Val Ser Leu Thr Gln Asp Val Ile Thr Thr Glu Gly Leu Lys Gln Ser 
        355                 360                 365             


Glu Asn Lys Leu Pro Glu Pro Arg Ser Phe Pro Lys Trp Phe Asn Ala 
    370                 375                 380                 


Glu Trp Met Trp Ala Ile Lys Asp Ser Asp Leu Thr Gly Trp Val Pro 
385                 390                 395                 400 


Met Ala Glu Tyr Pro Pro Ala Asp Asn Glu Leu Glu Asp Tyr Ala Glu 
                405                 410                 415     


His Leu Asn Lys Thr Met Glu Gly Val Leu Gln Gly Thr Asn Cys Ala 
            420                 425                 430         


Arg Glu Met Gly Lys Cys Ile Leu Thr Val Gly Ala Leu Met Thr Glu 
        435                 440                 445             


Cys Arg Leu Phe Pro Gly Lys Ile Lys Val Val Pro Ile Tyr Ala Arg 
    450                 455                 460                 


Ser Lys Glu Arg Lys Ser Met Gln Glu Gly Leu Pro Val Pro Ser Glu 
465                 470                 475                 480 


Met Asp Cys Leu Phe Gly Ile Cys Val Lys Ser Lys Ser His Leu Asn 
                485                 490                 495     


Lys Asp Asp Gly Met Tyr Thr Ile Ile Thr Phe Glu Phe Ser Ile Arg 
            500                 505                 510         


Glu Pro Asn Leu Glu Lys His Gln Lys Tyr Thr Val Phe Glu Ala Gly 
        515                 520                 525             


His Thr Thr Val Arg Met Lys Lys Gly Glu Ser Val Ile Gly Arg Glu 
    530                 535                 540                 


Val Pro Leu Tyr Leu Tyr Cys Arg Thr Thr Ala Leu Ser Lys Ile Lys 
545                 550                 555                 560 


Asn Asp Trp Leu Ser Lys Ala Arg Arg Cys Phe Ile Thr Thr Met Asp 
                565                 570                 575     


Thr Val Glu Thr Ile Cys Leu Arg Glu Ser Ala Lys Ala Glu Glu Asn 
            580                 585                 590         


Leu Val Glu Lys Thr Leu Asn Glu Lys Gln Met Trp Ile Gly Lys Lys 
        595                 600                 605             


Asn Gly Glu Leu Ile Ala Gln Pro Leu Arg Glu Ala Leu Arg Val Gln 
    610                 615                 620                 


Leu Val Gln Gln Phe Tyr Phe Cys Ile Tyr Asn Asp Ser Gln Leu Glu 
625                 630                 635                 640 


Gly Phe Cys Asn Glu Gln Lys Lys Ile Leu Met Ala Leu Glu Gly Asp 
                645                 650                 655     


Lys Lys Asn Lys Ser Ser Phe Gly Phe Asn Pro Glu Gly Leu Leu Glu 
            660                 665                 670         


Lys Ile Glu Glu Cys Leu Ile Asn Asn Pro Met Cys Leu Phe Met Ala 
        675                 680                 685             


Gln Arg Leu Asn Glu Leu Val Ile Glu Ala Ser Lys Arg Gly Ala Lys 
    690                 695                 700                 


Phe Phe Lys Ile Asp 
705                 


