                         SEQUENCE LISTING

<110>  Molecular Assembly, LLC
       Efcavitch, J. W.
       Siddiqi, Suhaib W.
 
<120>  METHODS AND APPARATUS FOR SYNTHESIZING NUCLEIC ACIDS

<130>  MOLA-001/01WO 31144/4

<150>  61/891162
<151>  2013-10-15

<150>  14/056687
<151>  2013-10-17

<150>  61/807327
<151>  2013-04-02

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  520
<212>  PRT
<213>  Bos taurus

<400>  1

Met Ala Gln Gln Arg Gln His Gln Arg Leu Pro Met Asp Pro Leu Cys 
1               5                   10                  15      


Thr Ala Ser Ser Gly Pro Arg Lys Lys Arg Pro Arg Gln Val Gly Ala 
            20                  25                  30          


Ser Met Ala Ser Pro Pro His Asp Ile Lys Phe Gln Asn Leu Val Leu 
        35                  40                  45              


Phe Ile Leu Glu Lys Lys Met Gly Thr Thr Arg Arg Asn Phe Leu Met 
    50                  55                  60                  


Glu Leu Ala Arg Arg Lys Gly Phe Arg Val Glu Asn Glu Leu Ser Asp 
65                  70                  75                  80  


Ser Val Thr His Ile Val Ala Glu Asn Asn Ser Gly Ser Glu Val Leu 
                85                  90                  95      


Glu Trp Leu Gln Val Gln Asn Ile Arg Ala Ser Ser Gln Leu Glu Leu 
            100                 105                 110         


Leu Asp Val Ser Trp Leu Ile Glu Ser Met Gly Ala Gly Lys Pro Val 
        115                 120                 125             


Glu Ile Thr Gly Lys His Gln Leu Val Val Arg Thr Asp Tyr Ser Ala 
    130                 135                 140                 


Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro Pro Leu Ala Val Lys Lys 
145                 150                 155                 160 


Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr Thr Leu Asn Asn Tyr Asn 
                165                 170                 175     


His Ile Phe Thr Asp Ala Phe Glu Ile Leu Ala Glu Asn Ser Glu Phe 
            180                 185                 190         


Lys Glu Asn Glu Val Ser Tyr Val Thr Phe Met Arg Ala Ala Ser Val 
        195                 200                 205             


Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser Met Lys Asp Thr Glu Gly 
    210                 215                 220                 


Ile Pro Cys Leu Gly Asp Lys Val Lys Cys Ile Ile Glu Glu Ile Ile 
225                 230                 235                 240 


Glu Asp Gly Glu Ser Ser Glu Val Lys Ala Val Leu Asn Asp Glu Arg 
                245                 250                 255     


Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val Phe Gly Val Gly Leu Lys 
            260                 265                 270         


Thr Ser Glu Lys Trp Phe Arg Met Gly Phe Arg Ser Leu Ser Lys Ile 
        275                 280                 285             


Met Ser Asp Lys Thr Leu Lys Phe Thr Lys Met Gln Lys Ala Gly Phe 
    290                 295                 300                 


Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val Thr Arg Ala Glu Ala Glu 
305                 310                 315                 320 


Ala Val Gly Val Leu Val Lys Glu Ala Val Trp Ala Phe Leu Pro Asp 
                325                 330                 335     


Ala Phe Val Thr Met Thr Gly Gly Phe Arg Arg Gly Lys Lys Ile Gly 
            340                 345                 350         


His Asp Val Asp Phe Leu Ile Thr Ser Pro Gly Ser Ala Glu Asp Glu 
        355                 360                 365             


Glu Gln Leu Leu Pro Lys Val Ile Asn Leu Trp Glu Lys Lys Gly Leu 
    370                 375                 380                 


Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr Phe Glu Lys Phe Lys Leu 
385                 390                 395                 400 


Pro Ser Arg Gln Val Asp Thr Leu Asp His Phe Gln Lys Cys Phe Leu 
                405                 410                 415     


Ile Leu Lys Leu His His Gln Arg Val Asp Ser Ser Lys Ser Asn Gln 
            420                 425                 430         


Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg Val Asp Leu Val Met Cys 
        435                 440                 445             


Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu Gly Trp Thr Gly Ser Arg 
    450                 455                 460                 


Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala Thr His Glu Arg Lys Met 
465                 470                 475                 480 


Met Leu Asp Asn His Ala Leu Tyr Asp Lys Thr Lys Arg Val Phe Leu 
                485                 490                 495     


Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala His Leu Gly Leu Asp Tyr 
            500                 505                 510         


Ile Glu Pro Trp Glu Arg Asn Ala 
        515                 520 


<210>  2
<211>  1923
<212>  DNA
<213>  Bos taurus

<400>  2
ctcttctgga gataccactt gatggcacag cagaggcagc atcagcgtct tcccatggat       60

ccgctgtgca cagcctcctc aggccctcgg aagaagagac ccaggcaggt gggtgcctca      120

atggcctccc ctcctcatga catcaagttt caaaatttgg tcctcttcat tttggagaag      180

aaaatgggaa ccacccgcag aaacttcctc atggagctgg ctcgaaggaa aggtttcagg      240

gttgaaaatg agctcagtga ttctgtcacc cacattgtag cagaaaacaa ctctggttca      300

gaggttctcg agtggcttca ggtacagaac ataagagcca gctcgcagct agaactcctt      360

gatgtctcct ggctgatcga aagtatggga gcaggaaaac cagtggagat tacaggaaaa      420

caccagcttg ttgtgagaac agactattca gctaccccaa acccaggctt ccagaagact      480

ccaccacttg ctgtaaaaaa gatctcccag tacgcgtgtc aaagaaaaac cactttgaac      540

aactataacc acatattcac ggatgccttt gagatactgg ctgaaaattc tgagtttaaa      600

gaaaatgaag tctcttatgt gacatttatg agagcagctt ctgtacttaa atctctgcca      660

ttcacaatca tcagtatgaa ggatacagaa ggaattccct gcctggggga caaggtgaag      720

tgtatcatag aggaaattat tgaagatgga gaaagttctg aagttaaagc tgtgttaaat      780

gatgaacgat atcagtcctt caaactcttt acttctgttt ttggagtggg actgaagaca      840

tctgagaaat ggttcaggat ggggttcaga tctctgagta aaataatgtc agacaaaacc      900

ctgaaattca caaaaatgca gaaagcagga tttctctatt atgaagacct tgtcagctgc      960

gtgaccaggg ccgaagcaga ggcggttggc gtgctggtta aagaggctgt gtgggcattt     1020

ctgccggatg cctttgtcac catgacagga ggattccgca ggggtaagaa gattgggcat     1080

gatgtagatt ttttaattac cagcccagga tcagcagagg atgaagagca acttttgcct     1140

aaagtgataa acttatggga aaaaaaggga ttacttttat attatgacct tgtggagtca     1200

acatttgaaa agttcaagtt gccaagcagg caggtggata ctttagatca ttttcaaaaa     1260

tgctttctga ttttaaaatt gcaccatcag agagtagaca gtagcaagtc caaccagcag     1320

gaaggaaaga cctggaaggc catccgtgtg gacctggtta tgtgccccta cgagaaccgt     1380

gcctttgccc tgctaggctg gactggctcc cggcagtttg agagagacat ccggcgctat     1440

gccacacacg agcggaagat gatgctggat aaccacgctt tatatgacaa gaccaagagg     1500

gtatttctca aagcggaaag tgaagaagaa atctttgcac atctgggatt ggactacatt     1560

gaaccatggg aaagaaatgc ttaggagaaa gctgtcaact tttttctttt ctgttctttt     1620

tttcaggtta gacaaattat gcttcatatt ataatgaaag atgccttagt caagtttggg     1680

attctttaca ttttaccaag atgtagattg cttctagaaa taagtagttt tggaaacgtg     1740

atcaggcacc ccctgggtta tgctctggca agccatttgc aggactgatg tgtagaactc     1800

gcaatgcatt ttccatagaa acagtgttgg aattggtggc tcatttccag ggaagttcat     1860

caaagcccac tttgcccaca gtgtagctga aatactgtat acttgccaat aaaaatagga     1920

aac                                                                   1923


<210>  3
<211>  465
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Phe Thr Arg Val Ala Asn Phe Cys Arg Lys Val Leu Ser Arg Glu 
1               5                   10                  15      


Glu Ser Glu Ala Glu Gln Ala Val Ala Arg Pro Gln Val Thr Val Ile 
            20                  25                  30          


Pro Arg Glu Gln His Ala Ile Ser Arg Lys Asp Ile Ser Glu Asn Ala 
        35                  40                  45              


Leu Lys Val Met Tyr Arg Leu Asn Lys Ala Gly Tyr Glu Ala Trp Leu 
    50                  55                  60                  


Val Gly Gly Gly Val Arg Asp Leu Leu Leu Gly Lys Lys Pro Lys Asp 
65                  70                  75                  80  


Phe Asp Val Thr Thr Asn Ala Thr Pro Glu Gln Val Arg Lys Leu Phe 
                85                  90                  95      


Arg Asn Cys Arg Leu Val Gly Arg Arg Phe Arg Leu Ala His Val Met 
            100                 105                 110         


Phe Gly Pro Glu Ile Ile Glu Val Ala Thr Phe Arg Gly His His Glu 
        115                 120                 125             


Gly Asn Val Ser Asp Arg Thr Thr Ser Gln Arg Gly Gln Asn Gly Met 
    130                 135                 140                 


Leu Leu Arg Asp Asn Ile Phe Gly Ser Ile Glu Glu Asp Ala Gln Arg 
145                 150                 155                 160 


Arg Asp Phe Thr Ile Asn Ser Leu Tyr Tyr Ser Val Ala Asp Phe Thr 
                165                 170                 175     


Val Arg Asp Tyr Val Gly Gly Met Lys Asp Leu Lys Asp Gly Val Ile 
            180                 185                 190         


Arg Leu Ile Gly Asn Pro Glu Thr Arg Tyr Arg Glu Asp Pro Val Arg 
        195                 200                 205             


Met Leu Arg Ala Val Arg Phe Ala Ala Lys Leu Gly Met Arg Ile Ser 
    210                 215                 220                 


Pro Glu Thr Ala Glu Pro Ile Pro Arg Leu Ala Thr Leu Leu Asn Asp 
225                 230                 235                 240 


Ile Pro Pro Ala Arg Leu Phe Glu Glu Ser Leu Lys Leu Leu Gln Ala 
                245                 250                 255     


Gly Tyr Gly Tyr Glu Thr Tyr Lys Leu Leu Cys Glu Tyr His Leu Phe 
            260                 265                 270         


Gln Pro Leu Phe Pro Thr Ile Thr Arg Tyr Phe Thr Glu Asn Gly Asp 
        275                 280                 285             


Ser Pro Met Glu Arg Ile Ile Glu Gln Val Leu Lys Asn Thr Asp Thr 
    290                 295                 300                 


Arg Ile His Asn Asp Met Arg Val Asn Pro Ala Phe Leu Phe Ala Ala 
305                 310                 315                 320 


Met Phe Trp Tyr Pro Leu Leu Glu Thr Ala Gln Lys Ile Ala Gln Glu 
                325                 330                 335     


Ser Gly Leu Thr Tyr His Asp Ala Phe Ala Leu Ala Met Asn Asp Val 
            340                 345                 350         


Leu Asp Glu Ala Cys Arg Ser Leu Ala Ile Pro Lys Arg Leu Thr Thr 
        355                 360                 365             


Leu Thr Arg Asp Ile Trp Gln Leu Gln Leu Arg Met Ser Arg Arg Gln 
    370                 375                 380                 


Gly Lys Arg Ala Trp Lys Leu Leu Glu His Pro Lys Phe Arg Ala Ala 
385                 390                 395                 400 


Tyr Asp Leu Leu Ala Leu Arg Ala Glu Val Glu Arg Asn Ala Glu Leu 
                405                 410                 415     


Gln Arg Leu Val Lys Trp Trp Gly Glu Phe Gln Val Ser Ala Pro Pro 
            420                 425                 430         


Asp Gln Lys Gly Met Leu Asn Glu Leu Asp Glu Glu Pro Ser Pro Arg 
        435                 440                 445             


Arg Arg Thr Arg Arg Pro Arg Lys Arg Ala Pro Arg Arg Glu Gly Thr 
    450                 455                 460                 


Ala 
465 


<210>  4
<211>  1398
<212>  DNA
<213>  Escherichia coli

<400>  4
atttttaccc gagtcgctaa tttttgccgc aaggtgctaa gccgcgagga aagcgaggct       60

gaacaggcag tcgcccgtcc acaggtgacg gtgatcccgc gtgagcagca tgctatttcc      120

cgcaaagata tcagtgaaaa tgccctgaag gtaatgtaca ggctcaataa agcgggatac      180

gaagcctggc tggttggcgg cggcgtgcgc gacctgttac ttggcaaaaa gccgaaagat      240

tttgacgtaa ccactaacgc cacgcctgag caggtgcgca aactgttccg taactgccgc      300

ctggtgggtc gccgtttccg tctggctcat gtaatgtttg gcccggagat tatcgaagtt      360

gcgaccttcc gtggacacca cgaaggtaac gtcagcgacc gcacgacctc ccaacgcggg      420

caaaacggca tgttgctgcg cgacaacatt ttcggctcca tcgaagaaga cgcccagcgc      480

cgcgatttca ctatcaacag cctgtattac agcgtagcgg attttaccgt ccgtgattac      540

gttggcggca tgaaggatct gaaggacggc gttatccgtc tgattggtaa cccggaaacg      600

cgctaccgtg aagatccggt acgtatgctg cgcgcggtac gttttgccgc caaattgggt      660

atgcgcatca gcccggaaac cgcagaaccg atccctcgcc tcgctaccct gctgaacgat      720

atcccaccgg cacgcctgtt tgaagaatcg cttaaactgc tacaagcggg ctacggttac      780

gaaacctata agctgttgtg tgaatatcat ctgttccagc cgctgttccc gaccattacc      840

cgctacttca cggaaaatgg cgacagcccg atggagcgga tcattgaaca ggtgctgaag      900

aataccgata cgcgtatcca taacgatatg cgcgtgaacc cggcgttcct gtttgccgcc      960

atgttctggt acccactgct ggagacggca cagaagatcg cccaggaaag cggcctgacc     1020

tatcacgacg ctttcgcgct ggcgatgaac gacgtgctgg acgaagcctg ccgttcactg     1080

gcaatcccga aacgtctgac gacattaacc cgcgatatct ggcagttgca gttgcgtatg     1140

tcccgtcgtc agggtaaacg cgcatggaaa ctgctggagc atcctaagtt ccgtgcggct     1200

tatgacctgt tggccttgcg agctgaagtt gagcgtaacg ctgaactgca gcgtctggtg     1260

aaatggtggg gtgagttcca ggtttccgcg ccaccagacc aaaaagggat gctcaacgag     1320

ctggatgaag aaccgtcacc gcgtcgtcgt actcgtcgtc cacgcaaacg cgcaccacgt     1380

cgtgagggta ccgcatga                                                   1398


<210>  5
<211>  349
<212>  PRT
<213>  Escherichia coli

<400>  5

Gly Ser His Met Ser Tyr Gln Lys Val Pro Asn Ser His Lys Glu Phe 
1               5                   10                  15      


Thr Lys Phe Cys Tyr Glu Val Tyr Asn Glu Ile Lys Ile Ser Asp Lys 
            20                  25                  30          


Glu Phe Lys Glu Lys Arg Ala Ala Leu Asp Thr Leu Arg Leu Cys Leu 
        35                  40                  45              


Lys Arg Ile Ser Pro Asp Ala Glu Leu Val Ala Phe Gly Ser Leu Glu 
    50                  55                  60                  


Ser Gly Leu Ala Leu Lys Asn Ser Asp Met Asp Leu Cys Val Leu Met 
65                  70                  75                  80  


Asp Ser Arg Val Gln Ser Asp Thr Ile Ala Leu Gln Phe Tyr Glu Glu 
                85                  90                  95      


Leu Ile Ala Glu Gly Phe Glu Gly Lys Phe Leu Gln Arg Ala Arg Ile 
            100                 105                 110         


Pro Ile Ile Lys Leu Thr Ser Asp Thr Lys Asn Gly Phe Gly Ala Ser 
        115                 120                 125             


Phe Gln Cys Asp Ile Gly Phe Asn Asn Arg Leu Ala Ile His Asn Thr 
    130                 135                 140                 


Leu Leu Leu Ser Ser Tyr Thr Lys Leu Asp Ala Arg Leu Lys Pro Met 
145                 150                 155                 160 


Val Leu Leu Val Lys His Trp Ala Lys Arg Lys Gln Ile Asn Ser Pro 
                165                 170                 175     


Tyr Phe Gly Thr Leu Ser Ser Tyr Gly Tyr Val Leu Met Val Leu Tyr 
            180                 185                 190         


Tyr Leu Ile His Val Ile Lys Pro Pro Val Phe Pro Asn Leu Leu Leu 
        195                 200                 205             


Ser Pro Leu Lys Gln Glu Lys Ile Val Asp Gly Phe Asp Val Gly Phe 
    210                 215                 220                 


Asp Asp Lys Leu Glu Asp Ile Pro Pro Ser Gln Asn Tyr Ser Ser Leu 
225                 230                 235                 240 


Gly Ser Leu Leu His Gly Phe Phe Arg Phe Tyr Ala Tyr Lys Phe Glu 
                245                 250                 255     


Pro Arg Glu Lys Val Val Thr Phe Arg Arg Pro Asp Gly Tyr Leu Thr 
            260                 265                 270         


Lys Gln Glu Lys Gly Trp Thr Ser Ala Thr Glu His Thr Gly Ser Ala 
        275                 280                 285             


Asp Gln Ile Ile Lys Asp Arg Tyr Ile Leu Ala Ile Glu Asp Pro Phe 
    290                 295                 300                 


Glu Ile Ser His Asn Val Gly Arg Thr Val Ser Ser Ser Gly Leu Tyr 
305                 310                 315                 320 


Arg Ile Arg Gly Glu Phe Met Ala Ala Ser Arg Leu Leu Asn Ser Arg 
                325                 330                 335     


Ser Tyr Pro Ile Pro Tyr Asp Ser Leu Phe Glu Glu Ala 
            340                 345                 


<210>  6
<211>  1047
<212>  DNA
<213>  Escherichia coli

<400>  6
ggcagccata tgagctatca gaaagtgccg aacagccata aagaatttac caaattttgc       60

tatgaagtgt ataacgaaat taaaattagc gataaagaat ttaaagaaaa acgcgcggcg      120

ctggataccc tgcgcctgtg cctgaaacgc attagcccgg atgcggaact ggtggcgttt      180

ggcagcctgg aaagcggcct ggcgctgaaa aacagcgata tggatctgtg cgtgctgatg      240

gatagccgcg tgcagagcga taccattgcg ctgcagtttt atgaagaact gattgcggaa      300

ggctttgaag gcaaatttct gcagcgcgcg cgcattccga ttattaaact gaccagcgat      360

accaaaaacg gctttggcgc gagctttcag tgcgatattg gctttaacaa ccgcctggcg      420

attcataaca ccctgctgct gagcagctat accaaactgg atgcgcgcct gaaaccgatg      480

gtgctgctgg tgaaacattg ggcgaaacgc aaacagatta acagcccgta ttttggcacc      540

ctgagcagct atggctatgt gctgatggtg ctgtattatc tgattcatgt gattaaaccg      600

ccggtgtttc cgaacctgct gctgagcccg ctgaaacagg aaaaaattgt ggatggcttt      660

gatgtgggct ttgatgataa actggaagat attccgccga gccagaacta tagcagcctg      720

ggcagcctgc tgcatggctt ttttcgcttt tatgcgtata aatttgaacc gcgcgaaaaa      780

gtggtgacct ttcgccgccc ggatggctat ctgaccaaac aggaaaaagg ctggaccagc      840

gcgaccgaac ataccggcag cgcggatcag attattaaag atcgctatat tctggcgatt      900

gaagatccgt ttgaaattag ccataacgtg ggccgcaccg tgagcagcag cggcctgtat      960

cgcattcgcg gcgaatttat ggcggcgagc cgcctgctga acagccgcag ctatccgatt     1020

ccgtatgata gcctgtttga agaagcg                                         1047


