                         SEQUENCE LISTING

<110>  Danforth
       Smith, Tom James
       Shah, Dilip Maganlal
 
<120>  KP6 ANTIFUNGAL PROTEIN-INDUCED FUNGAL RESISTANCE IN FOOD CROPS

<130>  DDPSC0026-401-PC

<150>  US 61/776253
<151>  2013-03-11

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  219
<212>  PRT
<213>  Ustilago maydis

<400>  1

Met Leu Ile Phe Ser Val Leu Met Tyr Leu Gly Leu Leu Leu Ala Gly 
1               5                   10                  15      


Ala Ser Ala Leu Pro Asn Gly Leu Ser Pro Arg Asn Asn Ala Phe Cys 
            20                  25                  30          


Ala Gly Phe Gly Leu Ser Cys Lys Trp Glu Cys Trp Cys Thr Ala His 
        35                  40                  45              


Gly Thr Gly Asn Glu Leu Arg Tyr Ala Thr Ala Ala Gly Cys Gly Asp 
    50                  55                  60                  


His Leu Ser Lys Ser Tyr Tyr Asp Ala Arg Ala Gly His Cys Leu Phe 
65                  70                  75                  80  


Ser Asp Asp Leu Arg Asn Gln Phe Tyr Ser His Cys Ser Ser Leu Asn 
                85                  90                  95      


Asn Asn Met Ser Cys Arg Ser Leu Ser Lys Arg Thr Ile Gln Asp Ser 
            100                 105                 110         


Ala Thr Asp Thr Val Asp Leu Gly Ala Glu Leu His Arg Asp Asp Pro 
        115                 120                 125             


Pro Pro Thr Ala Ser Asp Ile Gly Lys Arg Gly Lys Arg Pro Arg Pro 
    130                 135                 140                 


Val Met Cys Gln Cys Val Asp Thr Thr Asn Gly Gly Val Arg Leu Asp 
145                 150                 155                 160 


Ala Val Thr Arg Ala Ala Cys Ser Ile Asp Ser Phe Ile Asp Gly Tyr 
                165                 170                 175     


Tyr Thr Glu Lys Asp Gly Phe Cys Arg Ala Lys Tyr Ser Trp Asp Leu 
            180                 185                 190         


Phe Thr Ser Gly Gln Phe Tyr Gln Ala Cys Leu Arg Tyr Ser His Ala 
        195                 200                 205             


Gly Thr Asn Cys Gln Pro Asp Pro Gln Tyr Glu 
    210                 215                 


<210>  2
<211>  27
<212>  PRT
<213>  Ustilago maydis

<400>  2

Met Leu Ile Phe Ser Val Leu Met Tyr Leu Gly Leu Leu Leu Ala Gly 
1               5                   10                  15      


Ala Ser Ala Leu Pro Asn Gly Leu Ser Pro Arg 
            20                  25          


<210>  3
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MsDef1 Export Signal Amino Acid Sequence

<400>  3

Met Glu Lys Lys Ser Leu Ala Gly Leu Cys Phe Leu Phe Leu Val Leu 
1               5                   10                  15      


Phe Val Ala Gln Glu Ile Val Val Thr Glu Ala 
            20                  25          


<210>  4
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MsDef1 Export Signal Amino Acid Sequence Nucleotide Coding 
       Sequence

<400>  4
atggagaaga aatcactagc tggcttatgc ttcctcttct tggttctctt tgttgcacaa       60

gaaattgtgg tgacagaagc ca                                                82


<210>  5
<211>  192
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wild-Type U. maydis Amino Acid Core KP6 Protoxin Sequence Without
       N-Terminal Sequence

<400>  5

Asn Asn Ala Phe Cys Ala Gly Phe Gly Leu Ser Cys Lys Trp Glu Cys 
1               5                   10                  15      


Trp Cys Thr Ala His Gly Thr Gly Asn Glu Leu Arg Tyr Ala Thr Ala 
            20                  25                  30          


Ala Gly Cys Gly Asp His Leu Ser Lys Ser Tyr Tyr Asp Ala Arg Ala 
        35                  40                  45              


Gly His Cys Leu Phe Ser Asp Asp Leu Arg Asn Gln Phe Tyr Ser His 
    50                  55                  60                  


Cys Ser Ser Leu Asn Asn Asn Met Ser Cys Arg Ser Leu Ser Lys Arg 
65                  70                  75                  80  


Thr Ile Gln Asp Ser Ala Thr Asp Thr Val Asp Leu Gly Ala Glu Leu 
                85                  90                  95      


His Arg Asp Asp Pro Pro Pro Thr Ala Ser Asp Ile Gly Lys Arg Gly 
            100                 105                 110         


Lys Arg Pro Arg Pro Val Met Cys Gln Cys Val Asp Thr Thr Asn Gly 
        115                 120                 125             


Gly Val Arg Leu Asp Ala Val Thr Arg Ala Ala Cys Ser Ile Asp Ser 
    130                 135                 140                 


Phe Ile Asp Gly Tyr Tyr Thr Glu Lys Asp Gly Phe Cys Arg Ala Lys 
145                 150                 155                 160 


Tyr Ser Trp Asp Leu Phe Thr Ser Gly Gln Phe Tyr Gln Ala Cys Leu 
                165                 170                 175     


Arg Tyr Ser His Ala Gly Thr Asn Cys Gln Pro Asp Pro Gln Tyr Glu 
            180                 185                 190         


<210>  6
<211>  576
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wild-Type U. maydis Amino Acid Core KP6 Protoxin Sequence Without
       N-Terminal Sequence  Nucleotide Coding Sequence

<400>  6
aacaatgctt tttgtgctgg atttggtctc tcttgcaagt gggaatgttg gtgcacagca       60

cacggaacgg gcaatgaatt acggtatgct accgcagcag gatgcggaga tcatctgtcc      120

aagtcttatt acgatgctcg ggccggccac tgcctgttct ctgacgacct tcgcaaccag      180

ttctacagcc attgttcgtc tctaaacaac aatatgtcgt gccggtcgtt gtctaaacgg      240

actatccaag atagcgctac cgacacggta gacctcggtg ccgagctcca tagggatgac      300

ccgcccccta ctgctagtga cataggcaaa cggggtaaga ggcctagacc tgttatgtgc      360

caatgtgtag acacaacgaa cggaggggtt cgattagacg cggtgactag ggcggcttgc      420

agcatagact cgtttatcga cgggtactat acggaaaagg atgggttttg tagagctaaa      480

tattcctggg acttgtttac gagcggccag ttctaccagg catgtttgag gtactcacat      540

gccgggacca actgccaacc tgacccgcag tatgaa                                576


<210>  7
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric U. maydis KP6 Protoxin Protein Sequence With N-Terminal 
       MsDef1 Export Signal Amino Acid Sequence

<400>  7

Met Glu Lys Lys Ser Leu Ala Gly Leu Cys Phe Leu Phe Leu Val Leu 
1               5                   10                  15      


Phe Val Ala Gln Glu Ile Val Val Thr Glu Ala Asn Asn Ala Phe Cys 
            20                  25                  30          


Ala Gly Phe Gly Leu Ser Cys Lys Trp Glu Cys Trp Cys Thr Ala His 
        35                  40                  45              


Gly Thr Gly Asn Glu Leu Arg Tyr Ala Thr Ala Ala Gly Cys Gly Asp 
    50                  55                  60                  


His Leu Ser Lys Ser Tyr Tyr Asp Ala Arg Ala Gly His Cys Leu Phe 
65                  70                  75                  80  


Ser Asp Asp Leu Arg Asn Gln Phe Tyr Ser His Cys Ser Ser Leu Asn 
                85                  90                  95      


Asn Asn Met Ser Cys Arg Ser Leu Ser Lys Arg Thr Ile Gln Asp Ser 
            100                 105                 110         


Ala Thr Asp Thr Val Asp Leu Gly Ala Glu Leu His Arg Asp Asp Pro 
        115                 120                 125             


Pro Pro Thr Ala Ser Asp Ile Gly Lys Arg Gly Lys Arg Pro Arg Pro 
    130                 135                 140                 


Val Met Cys Gln Cys Val Asp Thr Thr Asn Gly Gly Val Arg Leu Asp 
145                 150                 155                 160 


Ala Val Thr Arg Ala Ala Cys Ser Ile Asp Ser Phe Ile Asp Gly Tyr 
                165                 170                 175     


Tyr Thr Glu Lys Asp Gly Phe Cys Arg Ala Lys Tyr Ser Trp Asp Leu 
            180                 185                 190         


Phe Thr Ser Gly Gln Phe Tyr Gln Ala Cys Leu Arg Tyr Ser His Ala 
        195                 200                 205             


Gly Thr Asn Cys Gln Pro Asp Pro Gln Tyr Glu 
    210                 215                 


<210>  8
<211>  657
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chimeric U. maydis KP6 Protoxin Protein Sequence With N-Terminal 
       MsDef1 Export Signal Amino Acid Sequence Nucleotide Coding 
       Sequence

<400>  8
atggagaaga aatcactagc tggcttatgc ttcctcttct tggttctctt tgttgcacaa       60

gaaattgtgg tgacagaagc caacaatgct ttttgtgctg gatttggtct ctcttgcaag      120

tgggaatgtt ggtgcacagc acacggaacg ggcaatgaat tacggtatgc taccgcagca      180

ggatgcggag atcatctgtc caagtcttat tacgatgctc gggccggcca ctgcctgttc      240

tctgacgacc ttcgcaacca gttctacagc cattgttcgt ctctaaacaa caatatgtcg      300

tgccggtcgt tgtctaaacg gactatccaa gatagcgcta ccgacacggt agacctcggt      360

gccgagctcc atagggatga cccgccccct actgctagtg acataggcaa acggggtaag      420

aggcctagac ctgttatgtg ccaatgtgta gacacaacga acggaggggt tcgattagac      480

gcggtgacta gggcggcttg cagcatagac tcgtttatcg acgggtacta tacggaaaag      540

gatgggtttt gtagagctaa atattcctgg gacttgttta cgagcggcca gttctaccag      600

gcatgtttga ggtactcaca tgccgggacc aactgccaac ctgacccgca gtatgaa         657


<210>  9
<211>  79
<212>  PRT
<213>  Ustilago maydis

<400>  9

Asn Asn Ala Phe Cys Ala Gly Phe Gly Leu Ser Cys Lys Trp Glu Cys 
1               5                   10                  15      


Trp Cys Thr Ala His Gly Thr Gly Asn Glu Leu Arg Tyr Ala Thr Ala 
            20                  25                  30          


Ala Gly Cys Gly Asp His Leu Ser Lys Ser Tyr Tyr Asp Ala Arg Ala 
        35                  40                  45              


Gly His Cys Leu Phe Ser Asp Asp Leu Arg Asn Gln Phe Tyr Ser His 
    50                  55                  60                  


Cys Ser Ser Leu Asn Asn Asn Met Ser Cys Arg Ser Leu Ser Lys 
65                  70                  75                  


<210>  10
<211>  237
<212>  DNA
<213>  Ustilago maydis

<400>  10
aacaatgctt tttgtgctgg atttggtctc tcttgcaagt gggaatgttg gtgcacagca       60

cacggaacgg gcaatgaatt acggtatgct accgcagcag gatgcggaga tcatctgtcc      120

aagtcttatt acgatgctcg ggccggccac tgcctgttct ctgacgacct tcgcaaccag      180

ttctacagcc attgttcgtc tctaaacaac aatatgtcgt gccggtcgtt gtctaaa         237


<210>  11
<211>  81
<212>  PRT
<213>  Ustilago maydis

<400>  11

Gly Lys Arg Pro Arg Pro Val Met Cys Gln Cys Val Asp Thr Thr Asn 
1               5                   10                  15      


Gly Gly Val Arg Leu Asp Ala Val Thr Arg Ala Ala Cys Ser Ile Asp 
            20                  25                  30          


Ser Phe Ile Asp Gly Tyr Tyr Thr Glu Lys Asp Gly Phe Cys Arg Ala 
        35                  40                  45              


Lys Tyr Ser Trp Asp Leu Phe Thr Ser Gly Gln Phe Tyr Gln Ala Cys 
    50                  55                  60                  


Leu Arg Tyr Ser His Ala Gly Thr Asn Cys Gln Pro Asp Pro Gln Tyr 
65                  70                  75                  80  


Glu 
    


<210>  12
<211>  247
<212>  DNA
<213>  Ustilago maydis

<400>  12
ggtaagaggc ctagacctgt tatgtgccaa tgtgtagaca caacgaacgg aggggttcga       60

ttagacgcgg tgactagggc ggcttgcagc atagactcgt ttatcgacgg gtactatacg      120

gaaaaggatg ggttttgtag agctaaatat tcctgggact tgtttacgag cggccagttc      180

taccaggcat gtttgaggta ctcacatgcc gggaccaact gccaacctga cccgcagtat      240

gaataag                                                                247


