
                                SEQUENCE LISTING

<110> IMAGINAB, INC.
      Ho, David T.
      Olafsen, Tove
      Agahi, Giti
      Gudas, Jean

<120> ANTIGEN BINDING CONSTRUCTS TO CD70
  

<130> IGNAB.022WO


<150> 61/777810           
<151> 2013-03-12 


<150> 61/779952           
<151> 2013-03-13 


<160> 27

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 333
<212> DNA
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 1
gatattgtga tgacccagag cccggatagc ctggcggtga gcctgggcga acgcgcgacc 60
attaactgcc gcgcgagcaa aagcgtgagc accagcggct atagctttat gcattggtat 120
cagcagaaac cgggccagcc gccgaaactg ctgatttatc tggcgagcaa cctggaaagc 180
ggcgtgccgg atcgctttag cggcagcggc agcggcaccg attttaccct gaccattagc 240
agcctgcagg cggaagatgt ggcggtgtat tattgccagc atagccgcga agtgccgtgg 300
acctttggcc agggcaccaa agtggaaatt aaa                              333

<210> 2
<211> 111
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 2
Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
 1               5                  10                  15      
Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Lys Ser Val Ser Thr Ser
            20                  25                  30          
Gly Tyr Ser Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro
        35                  40                  45              
Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Asp
    50                  55                  60                  
Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser
65                  70                  75                  80  
Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln His Ser Arg
                85                  90                  95      
Glu Val Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
            100                 105                 110     


<210> 3
<211> 354
<212> DNA
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 3
caggtgcagc tggtgcagag cggcgcggaa gtgaaaaaac cgggcgcgag cgtgaaagtg 60
agctgcaaag cgagcggcta tacctttacc aactatggca tgaactgggt gcgccaggcg 120
ccgggccagg gcctgaaatg gatgggctgg attaacacct ataccggcga accgacctat 180
gcggatgcgt ttaaaggccg cgtgaccatg acccgcgata ccagcattag caccgcgtat 240
atggaactga gccgcctgcg cagcgatgat accgcggtgt attattgcgc gcgcgattat 300
ggcgattatg gcatggatta ttggggccag ggcaccaccg tgaccgtgag cagc       354

<210> 4
<211> 118
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 4
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala
 1               5                  10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr
            20                  25                  30          
Gly Met Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Lys Trp Met
        35                  40                  45              
Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Ala Phe
    50                  55                  60                  
Lys Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr
65                  70                  75                  80  
Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ala Arg Asp Tyr Gly Asp Tyr Gly Met Asp Tyr Trp Gly Gln Gly Thr
            100                 105                 110         
Thr Val Thr Val Ser Ser
        115             


<210> 5
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 5
atggaaaccg acaccctgct gctgtgggtg ctgctgctct gggtcccagg ctccaccggt 60


<210> 6
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 6
Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro
 1               5                  10                  15      
Gly Ser Thr Gly
            20  


<210> 7
<211> 15
<212> DNA
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 7
agtggtggag gaggc                                                  15

<210> 8
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 8
Ser Gly Gly Gly Gly
 1               5  


<210> 9
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 9
ggcggaggga gtggcggagg cggc                                        24

<210> 10
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 10
Gly Gly Gly Ser Gly Gly Gly Gly
 1               5              


<210> 11
<211> 9
<212> DNA
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 11
ggcggctgc                                                         9

<210> 12
<211> 3
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 12
Gly Gly Cys
 1          


<210> 13
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 13
atggaaaccg acaccctgct gctgtgggtg ctgctgctct gggtcccagg ctccaccggt 60


<210> 14
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 14
Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro
 1               5                  10                  15      
Gly Ser Thr Gly
            20  


<210> 15
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 15
ggctccacat ccggcggagg ctctggcggt ggatctggcg gaggcggctc atcc       54

<210> 16
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 16
Gly Ser Thr Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly
 1               5                  10                  15      
Ser Ser
        


<210> 17
<211> 393
<212> DNA
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 17
gagcctaagt cctgcgacaa gacccacacc tgtccccctt gcggcggagg aagcagcgga 60
ggcggatccg gtggccagcc tcgggagcct caggtgtaca ccctgcctcc ctcccgggac 120
gagctgacca agaaccaggt gtccctgacc tgtctggtca agggcttcta cccttccgat 180
atcgccgtgg agtgggagtc caacggccag cctgagaaca actacaagac cacccctcct 240
gtgctggact ccgacggctc cttcttcctg tactccaagc tcacagtgga taagtcccgg 300
tggcagcagg gcaacgtgtt ctcctgctcc gtgatgcacg aggccctgca caaccactat 360
acccagaagt ccctgtccct gtctcctggc aag                              393

<210> 18
<211> 131
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 18
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Gly Gly
 1               5                  10                  15      
Gly Ser Ser Gly Gly Gly Ser Gly Gly Gln Pro Arg Glu Pro Gln Val
            20                  25                  30          
Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser
        35                  40                  45              
Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu
    50                  55                  60                  
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro
65                  70                  75                  80  
Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val
                85                  90                  95      
Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
            100                 105                 110         
His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
        115                 120                 125             
Pro Gly Lys
    130     


<210> 19
<211> 23
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 19
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala
 1               5                  10                  15      
Pro Glu Leu Leu Gly Gly Pro
            20              


<210> 20
<211> 25
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 20
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Gly
 1               5                  10                  15      
Gly Gly Ser Ser Gly Gly Gly Ser Gly
            20                  25  


<210> 21
<211> 19
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 21
Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val
 1               5                  10                  15      
Ala Gly Pro
            


<210> 22
<211> 70
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 22
Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro Arg Cys
 1               5                  10                  15      
Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro
            20                  25                  30          
Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Glu
        35                  40                  45              
Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Ala Pro
    50                  55                  60                  
Glu Leu Leu Gly Gly Pro
65                  70  


<210> 23
<211> 25
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 23
Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro Arg Cys
 1               5                  10                  15      
Pro Ala Pro Glu Leu Leu Gly Gly Pro
            20                  25  


<210> 24
<211> 23
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 24
Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Ala
 1               5                  10                  15      
Pro Glu Leu Leu Gly Gly Pro
            20              


<210> 25
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 25
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe
 1               5                  10                  15      
Leu Gly Gly Pro
            20  


<210> 26
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> Antigen binding constructs or fragments thereof

<400> 26
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe
 1               5                  10                  15      
Leu Gly Gly Pro
            20  


<210> 27
<211> 193
<212> PRT
<213> Homo sapiens

<400> 27
Met Pro Glu Glu Gly Ser Gly Cys Ser Val Arg Arg Arg Pro Tyr Gly
 1               5                  10                  15      
Cys Val Leu Arg Ala Ala Leu Val Pro Leu Val Ala Gly Leu Val Ile
            20                  25                  30          
Cys Leu Val Val Cys Ile Gln Arg Phe Ala Gln Ala Gln Gln Gln Leu
        35                  40                  45              
Pro Leu Glu Ser Leu Gly Trp Asp Val Ala Glu Leu Gln Leu Asn His
    50                  55                  60                  
Thr Gly Pro Gln Gln Asp Pro Arg Leu Tyr Trp Gln Gly Gly Pro Ala
65                  70                  75                  80  
Leu Gly Arg Ser Phe Leu His Gly Pro Glu Leu Asp Lys Gly Gln Leu
                85                  90                  95      
Arg Ile His Arg Asp Gly Ile Tyr Met Val His Ile Gln Val Thr Leu
            100                 105                 110         
Ala Ile Cys Ser Ser Thr Thr Ala Ser Arg His His Pro Thr Thr Leu
        115                 120                 125             
Ala Val Gly Ile Cys Ser Pro Ala Ser Arg Ser Ile Ser Leu Leu Arg
    130                 135                 140                 
Leu Ser Phe His Gln Gly Cys Thr Ile Ala Ser Gln Arg Leu Thr Pro
145                 150                 155                 160 
Leu Ala Arg Gly Asp Thr Leu Cys Thr Asn Leu Thr Gly Thr Leu Leu
                165                 170                 175     
Pro Ser Arg Asn Thr Asp Glu Thr Phe Phe Gly Val Gln Trp Val Arg
            180                 185                 190         
Pro
    

