                         SEQUENCE LISTING

<110>  The University Court Of The University Of Edinburgh
 
<120>  Enhanced Expression

<130>  P206806WO

<150>  GB 1305361.6
<151>  2013-03-25

<160>  34    

<170>  PatentIn version 3.5

<210>  1
<211>  1245
<212>  RNA
<213>  Echovirus 7

<400>  1
gucgacuccg uggugcccgu caacaauauc aaagucaacc ugcaaagcau ggaugcguau       60

cauauugagg ucaauaccgg gaaccaccag ggggaaaaga uuuuugcguu ccaaaugcag      120

ccgggguuag agucuguuuu caagagaacc cuuauggggg agauucuuaa uuauuaugca      180

cacuggucag ggagcauuaa gcugacauuc acauuuugug gaucggcgau ggcaacugga      240

aaacucuugu uagcguauuc accaccaggu gcugaugugc ccgcgaccag gaaacaggcg      300

auguuaggca cacacaugau uugggauauc gggcuucagu cgagcugugu uuugugcauc      360

ccauggauaa gucagacaca cuaccgguua gugcaacaag augaauacac gagugcaggc      420

aaugugacgu guugguacca aacaggaaua guggugcccc cuggcacucc aaauaagugu      480

guagugcuuu guuuugcauc agcuuguaau gauuucucag uucgaaugcu uagggacacc      540

ccuuucaucg gacaaacagc acugcugcaa ggcgacaccg aaacggcuau ugacaaugca      600

aucgccaggg uagcagauac gguggcgagc gguccuagua auucgaccag uaucccagca      660

cucacagcag uugagacagg ucacacguca caagucgagc ccagcgauac aaugcagacu      720

agacauguca aaaacuacca cucgcguucu gagucaaccg uggaaaacuu ucuaagucgc      780

uccgcuugug uguacaucga agaguacuac accaaggacc aagacaaugu uaauagguac      840

augucgugga caauaaaugc cagaagaaug gugcaauuga ggagaaaguu ugagcuguuu      900

acauacauga gauuugauau ggaaaucacg uuuguaauca caaguagaca acuaccuggg      960

acuagcauag cacaagauau gccgccacuc acccaccaga ucauguacau accaccaggu     1020

ggcccgguac caaacagcgu aacagauuuu gcguggcaga caucaacaaa ccccagcauu     1080

uucuggacag aaggaaacgc gccaccucgc augucuauuc cauucaucag uauuggcaau     1140

gcauauagca acuucuauga cgggugguca cacuuuuccc aaaacggugu guacggauac     1200

aacgcccuga acaacauggg caagcuguac gcacgucaug uuaac                     1245


<210>  2
<211>  1029
<212>  RNA
<213>  Echovirus 7

<400>  2
gaauucgccg uugcuaugau gaagagaaac ucaaguacag ugaagacuga guauggugag       60

uuuacuaugc ugggcaucua ugacaagugg gccguuuugc cacgccaugc uaaaccugga      120

ccaaccaucc ugaugaauga ccaagagguc ggcguguuag acgccaagga acuaguggac      180

aaggauggca cuaaccugga gcugacacua cucaaguuaa accggaauga gaaguucaga      240

gacaucagag gcuucuuggc uaaggaggaa guggaaguca acgaggcugu gcuggcaaua      300

aacacuagca aguuuccuaa cauguacauu ccaguagggc agguuacaga uuacggcuuc      360

cuaaaccugg gugguacacc caccaaaaga augcuuaugu auaacuuccc cacaagagca      420

ggccagugug gcgggguacu cauguccacu ggcaaaguuu ugggaaucca uguuggugga      480

aauggccauc aaggcuucuc agcagcacuu cucaaacacu acuuuaauga ugaacaagga      540

gagauugagu ucauugagag uucaaaggaa gcaggguucc caaucauuaa cgcacccagu      600

aaaaccaagc uggagccaag ugucuuccac caaguauuug aaggcaacaa agagccagca      660

guccucagga acagugaccc acgucucaaa gcuaauuucg aggaggccau cuuuuccaaa      720

uacauuggga augucaacac acacauagau gaauacaugu uggaggcugu ugaccauuau      780

gccggacaau uggccacccu agauaucagc acugaaccaa ugaaguugga ggaugcugug      840

uacgguacug aaggccuuga agcucuugac uuaacaacaa gugcaggcua ccccuauguc      900

gcacugggua ucaagaagag agacauccuc ucgaagaaga ccaaggaccu gaccaagcug      960

aaagagugca uggauaagua uggccugaau cuaccaaugg ugacauacgu gaaagaugaa     1020

cucagaucu                                                             1029


<210>  3
<211>  1245
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  CpG-low Region 1

<400>  3
gucgacucag uggugccagu caacaauauc aaagucaacc ugcaaagcau ggaugcuuau       60

cauauugagg ucaauacagg gaaccaccag ggggaaaaga uuuuugcuuu ccaaaugcag      120

ccuggguuag agucuguuuu caagagaacc cuuauggggg agauucuuaa uuauuaugca      180

cacuggucag ggagcauuaa gcugacauuc acauuuugug gaucugccau ggcaacugga      240

aaacucuugu uagcuuauuc accaccaggu gcugaugugc cugcaaccag gaaacaggcu      300

auguuaggca cacacaugau uugggauaua gggcuucagu ccagcugugu uuugugcauc      360

ccauggauaa gucagacaca cuacagguua gugcaacaag augaauacac aagugcaggc      420

aaugugacau guugguacca aacaggaaua guggugcccc cuggcacucc aaauaagugu      480

guagugcuuu guuuugcauc agcuuguaau gauuucucag uuaggaugcu uagggacacc      540

ccuuucauag gacaaacagc acugcugcaa ggagacacag aaacagcuau ugacaaugca      600

auugccaggg uagcagauac uguggcaagu gguccuagua auucaaccag uaucccagca      660

cucacagcag uugagacagg ucacaccuca caaguggagc ccagugauac aaugcagacu      720

agacauguca aaaacuacca cucuaggucu gagucaacug uggaaaacuu ucuaaguagg      780

ucagcuugug uguacauaga agaguacuac accaaggacc aagacaaugu uaauagguac      840

auguccugga caauaaaugc cagaagaaug gugcaauuga ggagaaaguu ugagcuguuu      900

acauacauga gauuugauau ggaaaucacc uuuguaauca caaguagaca acuaccuggg      960

acuagcauag cacaagauau gccaccacuc acccaccaga ucauguacau accaccaggu     1020

ggcccaguac caaacagugu aacagauuuu gccuggcaga caucaacaaa ccccagcauu     1080

uucuggacag aaggaaaugc cccaccuagg augucuauuc cauucaucag uauuggcaau     1140

gcauauagca acuucuauga ugggugguca cacuuuuccc aaaauggugu guauggauac     1200

aaugcccuga acaacauggg caagcuguau gcaagacaug uuaac                     1245


<210>  4
<211>  1029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  CpG-low Region 2

<400>  4
gaauucgcug uugcuaugau gaagagaaac ucaaguacag ugaagacuga guauggugag       60

uuuacuaugc ugggcaucua ugacaagugg gcaguuuugc caaggcaugc uaaaccugga      120

ccaaccaucc ugaugaauga ccaagagguu gggguguuag augccaagga acuaguggac      180

aaggauggca cuaaccugga gcugacacua cucaaguuaa acagaaauga gaaguucaga      240

gacaucagag gcuucuuggc uaaggaggaa guggaaguca augaggcugu gcuggcaaua      300

aacacuagca aguuuccuaa cauguacauu ccaguagggc agguuacaga uuauggcuuc      360

cuaaaccugg gugguacacc caccaaaaga augcuuaugu auaacuuccc cacaagagca      420

ggccagugug gagggguacu cauguccacu ggcaaaguuu ugggaaucca uguuggugga      480

aauggccauc aaggcuucuc agcagcacuu cucaaacacu acuuuaauga ugaacaagga      540

gagauugagu ucauugagag uucaaaggaa gcaggguucc caaucauuaa ugcacccagu      600

aaaaccaagc uggagccaag ugucuuccac caaguauuug aaggcaacaa agagccagca      660

guccucagga acagugaccc aaggcucaaa gcuaauuuug aggaggccau cuuuuccaaa      720

uacauuggga augucaacac acacauagau gaauacaugu uggaggcugu ugaccauuau      780

gcaggacaau uggccacccu agauaucagc acugaaccaa ugaaguugga ggaugcugug      840

uaugguacug aaggccuuga agcucuugac uuaacaacaa gugcaggcua ccccuaugug      900

gcacugggua ucaagaagag agacauccuc ucaaagaaga ccaaggaccu gaccaagcug      960

aaagagugca uggauaagua uggccugaau cuaccaaugg ugacauaugu gaaagaugaa     1020

cucagaucu                                                             1029


<210>  5
<211>  1245
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  UpA-low Region 1

<400>  5
gucgacuccg uggugcccgu caacaacauc aaagucaacc ugcaaagcau ggaugcguau       60

cacauugagg ucaacaccgg gaaccaccag ggggaaaaga uuuuugcguu ccaaaugcag      120

ccgggguugg agucuguuuu caagagaacc cucauggggg agauucucaa uuauuaugca      180

cacuggucag ggagcaucaa gcugacauuc acauuuugug gaucggcgau ggcaacugga      240

aaacucuugu uggcguauuc accaccaggu gcugaugugc ccgcgaccag gaaacaggcg      300

auguugggca cacacaugau uugggacauc gggcuucagu cgagcugugu uuugugcauc      360

ccauggauca gucagacaca cuaccgguug gugcaacaag augaauacac gagugcaggc      420

aaugugacgu guugguacca aacaggaauu guggugcccc cuggcacucc aaacaagugu      480

gucgugcuuu guuuugcauc agcuugcaau gauuucucag uucgaaugcu gagggacacc      540

ccuuucaucg gacaaacagc acugcugcaa ggcgacaccg aaacggcgau ugacaaugca      600

aucgccaggg uugcagacac gguggcgagc gguccgagca auucgaccag caucccagca      660

cucacagcag uugagacagg ucacacguca caagucgagc ccagcgacac aaugcagacc      720

agacauguca aaaacuacca cucgcguucu gagucaaccg uggaaaacuu ucucagucgc      780

uccgcuugug uguacaucga agaguacuac accaaggacc aagacaaugu caacagguac      840

augucgugga caaucaaugc cagaagaaug gugcaauuga ggagaaaguu ugagcuguuc      900

acauacauga gauuugacau ggaaaucacg uuugucauca caagcagaca acuuccuggg      960

acgagcaucg cacaagacau gccgccacuc acccaccaga ucauguacau cccaccaggu     1020

ggcccggucc caaacagcgu cacagauuuu gcguggcaga caucaacaaa ccccagcauu     1080

uucuggacag aaggaaacgc gccaccucgc auguccauuc cauucaucag cauuggcaau     1140

gcauacagca acuucuauga cgggugguca cacuuuuccc aaaacggugu guacggauac     1200

aacgcccuga acaacauggg caagcuguac gcacgucaug uuaac                     1245


<210>  6
<211>  1029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  UpA-low Region 2

<400>  6
gaauucgccg uugccaugau gaagagaaac ucaagcacag ugaagacuga guauggugag       60

uucacgaugc ugggcaucua ugacaagugg gccguuuugc cacgccaugc caaaccugga      120

ccaaccaucc ugaugaauga ccaagagguc ggcguguugg acgccaagga acugguggac      180

aaggauggca caaaccugga gcugacacuc cucaaguuga accggaauga gaaguucaga      240

gacaucagag gcuucuuggc gaaggaggaa guggaaguca acgaggcugu gcuggcaauc      300

aacaccagca aguuuccaaa cauguacauu ccaguugggc aggucacaga uuacggcuuc      360

cugaaccugg gugggacacc caccaaaaga augcucaugu acaacuuccc cacaagagca      420

ggccagugug gcggggugcu cauguccacu ggcaaaguuu ugggaaucca uguuggugga      480

aauggccauc aaggcuucuc agcagcacuu cucaaacacu acuucaauga ugaacaagga      540

gagauugagu ucauugagag uucaaaggaa gcaggguucc caaucaucaa cgcacccagc      600

aaaaccaagc uggagccaag ugucuuccac caaguguuug aaggcaacaa agagccagca      660

guccucagga acagugaccc acgucucaaa gccaauuucg aggaggccau cuuuuccaaa      720

uacauuggga augucaacac acacaucgau gaauacaugu uggaggcugu ugaccauuau      780

gccggacaau uggccacccu ugacaucagc acugaaccaa ugaaguugga ggaugcugug      840

uacggcacug aaggccuuga agcucuugac uugacaacaa gugcaggcua ccccuauguc      900

gcacugggga ucaagaagag agacauccuc ucgaagaaga ccaaggaccu gaccaagcug      960

aaagagugca uggacaagua uggccugaau cuuccaaugg ugacauacgu gaaagaugaa     1020

cucagaucu                                                             1029


<210>  7
<211>  1245
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  CpG and UpA-low Region 1

<400>  7
gucgacucag uggugccagu caacaacauc aaagucaacc ugcaaagcau ggaugcuuau       60

cacauugagg ucaacacagg gaaccaccag ggggaaaaga uuuuugcuuu ccaaaugcag      120

ccuggguugg agucuguuuu caagagaacc cugauggggg agauucugaa uuauuaugca      180

cacuggucag ggagcaucaa gcugacauuc acauuuugug gaucugccau ggcaacugga      240

aaacucuugu uggcuuauuc accaccaggu gcugaugugc cugcaaccag gaaacaggcc      300

auguugggca cacacaugau uugggacauu gggcuucagu ccagcugugu uuugugcauc      360

ccauggauca gucagacaca cuacagguug gugcaacaag augaauacac aagugcaggc      420

aaugugacau guugguacca aacaggaauu guggugcccc cuggcacucc aaacaagugu      480

guugugcuuu guuuugcauc agcuugcaau gauuucucag ucaggaugcu cagggacacc      540

ccuuucauug gacaaacagc acugcugcaa ggagacacag aaacagccau ugacaaugca      600

auugccaggg uugcagacac uguggcaagu gguccaagca auucaaccag caucccagca      660

cucacagcag uugagacagg ucacaccuca caaguggagc ccagugacac aaugcagaca      720

agacauguca aaaacuacca cuccaggucu gagucaacug uggaaaacuu ucucagcagg      780

ucagcuugug uguacauuga agaguacuac accaaggacc aagacaaugu caacagguac      840

auguccugga caaucaaugc cagaagaaug gugcaauuga ggagaaaguu ugagcuguuc      900

acauacauga gauuugacau ggaaaucacc uuugugauca caagcagaca acucccuggg      960

acaagcauug cacaagacau gccaccacuc acccaccaga ucauguacau uccaccaggu     1020

ggcccagugc caaacagugu cacagauuuu gccuggcaga caucaacaaa ccccagcauu     1080

uucuggacag aaggaaaugc cccaccaagg auguccauuc cauucaucag cauuggcaau     1140

gcauacagca acuucuauga ugggugguca cacuuuuccc aaaauggugu guauggauac     1200

aaugcccuga acaacauggg caagcuguau gcaagacaug uuaac                     1245


<210>  8
<211>  1029
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  CpG and UpA-low Region 2

<400>  8
gaauucgcug uugccaugau gaagagaaac ucaagcacag ugaagacuga guauggugag       60

uucaccaugc ugggcaucua ugacaagugg gcaguuuugc caaggcaugc caaaccugga      120

ccaaccaucc ugaugaauga ccaagagguu gggguguugg augccaagga acugguggac      180

aaggauggca ccaaccugga gcugacacuu cucaaguuga acagaaauga gaaguucaga      240

gacaucagag gcuucuuggc caaggaggaa guggaaguca augaggcugu gcuggcaauc      300

aacaccagca aguuucccaa cauguacauu ccaguggggc aggugacaga uuauggcuuc      360

cugaaccugg guggaacacc caccaaaaga augcucaugu acaacuuccc cacaagagca      420

ggccagugug gagggguucu cauguccacu ggcaaaguuu ugggaaucca uguuggugga      480

aauggccauc aaggcuucuc agcagcacuu cucaaacacu acuucaauga ugaacaagga      540

gagauugagu ucauugagag uucaaaggaa gcaggguucc caaucaucaa ugcacccagc      600

aaaaccaagc uggagccaag ugucuuccac caaguguuug aaggcaacaa agagccagca      660

guccucagga acagugaccc aaggcucaaa gccaauuuug aggaggccau cuuuuccaaa      720

uacauuggga augucaacac acacauugau gaauacaugu uggaggcugu ugaccauuau      780

gcaggacaau uggccacccu ggacaucagc acugaaccaa ugaaguugga ggaugcugug      840

uauggcacug aaggccuuga agcucuugac uugacaacaa gugcaggcua ccccuaugug      900

gcacugggga ucaagaagag agacauccuc ucaaagaaga ccaaggaccu gaccaagcug      960

aaagagugca uggacaagua uggccugaau cucccaaugg ugacauaugu gaaagaugaa     1020

cucagaucu                                                             1029


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E7 qRT-PCR sense primer

<400>  9
tccggcccct gaatgcggct aa                                                22


<210>  10
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E7 qRT-PCR antisense primer

<400>  10
cacccaaagt agtcggttcc gc                                                22


<210>  11
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  outer, sense sequencing primer

<400>  11
cccaatttga tgtaacacca cacatgg                                           27


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  inner, sense sequencing primer

<400>  12
gatattccag gcgaagtaca caacc                                             25


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EV7 WT, outer, antisense sequencing primer

<400>  13
caaagcacta cacacttatt tggag                                             25


<210>  14
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R1/R2 CpG-high, outer, antisense sequencing primer

<400>  14
attcgaacgg agaaatcgtt ac                                                22


<210>  15
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R1/R2 UpA-high, outer, antisense sequencing primer

<400>  15
tcccttagca tacgtactga gaaat                                             25


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EV7 WT, inner, antisense sequencing primer

<400>  16
gcaccactat tcctgtttgg t                                                 21


<210>  17
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R1/R2 CpG-high, inner, antisense sequencing primer

<400>  17
aacaaagcac gacgcactta tt                                                22


<210>  18
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R1/R2 UpA-high, inner, antisense sequencing primer

<400>  18
cattacaagc tgatgcaaaa catag                                             25


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  outer, sense sequencing primer

<400>  19
tgagcccgta catcaaatca                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  inner, sense sequencing primer

<400>  20
ttttaacccc acgaacctga                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  outer, antisense sequencing primer

<400>  21
ttgccgagtt gttcgacata                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  inner, antisense sequencing primer

<400>  22
caagtcacgg atgtctgcaa                                                   20


<210>  23
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  region 1 sense (outer) PCR primer

<400>  23
cccaatttga tgtaacacca cacatgg                                           27


<210>  24
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  region 1 sense (inner) PCR primer

<400>  24
gatattccag gcgaagtaca caacc                                             25


<210>  25
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  region 1 antisense (outer) PCR primer

<400>  25
cccatactcg gatgtgcttg gg                                                22


<210>  26
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  region 1 antisense (inner) PCR primer

<400>  26
cactcggatt gtgcttgaca tctg                                              24


<210>  27
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  region 2 sense (outer) PCR primer

<400>  27
caaggagcat acacaggaat acc                                               23


<210>  28
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  region 2 sense (inner) PCR primer

<400>  28
ggtacctact cttaggcaag ca                                                22


<210>  29
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  region 2 antisense (outer) PCR primer

<400>  29
gaatgtctgc ctcatcgcca act                                               23


<210>  30
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  region 2 antisense (inner) PCR primer

<400>  30
aagctggacg cttcaatgag cct                                               23


<210>  31
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH qRT-PCR sense primer

<400>  31
gaaatcccat caccatcttc cagg                                              24


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH qRT-PCR antisense primer

<400>  32
gagccccagc cttctccatg                                                   20


<210>  33
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IFN-beta qRT-PCR sense primer

<400>  33
gaccaacaag tgtctcctcc aaa                                               23


<210>  34
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IFN-beta qRT-PCR antisense primer

<400>  34
gaactgctgc agctgcttaa tc                                                22


