                         LISTADO DE SECUENCIAS

<110>  Universidad Pablo de Olavide
 
<120>  "USO DE INHIBIDORES DE SULFATASAS ESTEROIDEAS PARA EL TRATAMIENTO
        DEL ENVEJECIMIENTO"

<130>  ES2762.3

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  583
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Pro Leu Arg Lys Met Lys Ile Pro Phe Leu Leu Leu Phe Phe Leu 
1               5                   10                  15      


Trp Glu Ala Glu Ser His Ala Ala Ser Arg Pro Asn Ile Ile Leu Val 
            20                  25                  30          


Met Ala Asp Asp Leu Gly Ile Gly Asp Pro Gly Cys Tyr Gly Asn Lys 
        35                  40                  45              


Thr Ile Arg Thr Pro Asn Ile Asp Arg Leu Ala Ser Gly Gly Val Lys 
    50                  55                  60                  


Leu Thr Gln His Leu Ala Ala Ser Pro Leu Cys Thr Pro Ser Arg Ala 
65                  70                  75                  80  


Ala Phe Met Thr Gly Arg Tyr Pro Val Arg Ser Gly Met Ala Ser Trp 
                85                  90                  95      


Ser Arg Thr Gly Val Phe Leu Phe Thr Ala Ser Ser Gly Gly Leu Pro 
            100                 105                 110         


Thr Asp Glu Ile Thr Phe Ala Lys Leu Leu Lys Asp Gln Gly Tyr Ser 
        115                 120                 125             


Thr Ala Leu Ile Gly Lys Trp His Leu Gly Met Ser Cys His Ser Lys 
    130                 135                 140                 


Thr Asp Phe Cys His His Pro Leu His His Gly Phe Asn Tyr Phe Tyr 
145                 150                 155                 160 


Gly Ile Ser Leu Thr Asn Leu Arg Asp Cys Lys Pro Gly Glu Gly Ser 
                165                 170                 175     


Val Phe Thr Thr Gly Phe Lys Arg Leu Val Phe Leu Pro Leu Gln Ile 
            180                 185                 190         


Val Gly Val Thr Leu Leu Thr Leu Ala Ala Leu Asn Cys Leu Gly Leu 
        195                 200                 205             


Leu His Val Pro Leu Gly Val Phe Phe Ser Leu Leu Phe Leu Ala Ala 
    210                 215                 220                 


Leu Ile Leu Thr Leu Phe Leu Gly Phe Leu His Tyr Phe Arg Pro Leu 
225                 230                 235                 240 


Asn Cys Phe Met Met Arg Asn Tyr Glu Ile Ile Gln Gln Pro Met Ser 
                245                 250                 255     


Tyr Asp Asn Leu Thr Gln Arg Leu Thr Val Glu Ala Ala Gln Phe Ile 
            260                 265                 270         


Gln Arg Asn Thr Glu Thr Pro Phe Leu Leu Val Leu Ser Tyr Leu His 
        275                 280                 285             


Val His Thr Ala Leu Phe Ser Ser Lys Asp Phe Ala Gly Lys Ser Gln 
    290                 295                 300                 


His Gly Val Tyr Gly Asp Ala Val Glu Glu Met Asp Trp Ser Val Gly 
305                 310                 315                 320 


Gln Ile Leu Asn Leu Leu Asp Glu Leu Arg Leu Ala Asn Asp Thr Leu 
                325                 330                 335     


Ile Tyr Phe Thr Ser Asp Gln Gly Ala His Val Glu Glu Val Ser Ser 
            340                 345                 350         


Lys Gly Glu Ile His Gly Gly Ser Asn Gly Ile Tyr Lys Gly Gly Lys 
        355                 360                 365             


Ala Asn Asn Trp Glu Gly Gly Ile Arg Val Pro Gly Ile Leu Arg Trp 
    370                 375                 380                 


Pro Arg Val Ile Gln Ala Gly Gln Lys Ile Asp Glu Pro Thr Ser Asn 
385                 390                 395                 400 


Met Asp Ile Phe Pro Thr Val Ala Lys Leu Ala Gly Ala Pro Leu Pro 
                405                 410                 415     


Glu Asp Arg Ile Ile Asp Gly Arg Asp Leu Met Pro Leu Leu Glu Gly 
            420                 425                 430         


Lys Ser Gln Arg Ser Asp His Glu Phe Leu Phe His Tyr Cys Asn Ala 
        435                 440                 445             


Tyr Leu Asn Ala Val Arg Trp His Pro Gln Asn Ser Thr Ser Ile Trp 
    450                 455                 460                 


Lys Ala Phe Phe Phe Thr Pro Asn Phe Asn Pro Val Gly Ser Asn Gly 
465                 470                 475                 480 


Cys Phe Ala Thr His Val Cys Phe Cys Phe Gly Ser Tyr Val Thr His 
                485                 490                 495     


His Asp Pro Pro Leu Leu Phe Asp Ile Ser Lys Asp Pro Arg Glu Arg 
            500                 505                 510         


Asn Pro Leu Thr Pro Ala Ser Glu Pro Arg Phe Tyr Glu Ile Leu Lys 
        515                 520                 525             


Val Met Gln Glu Ala Ala Asp Arg His Thr Gln Thr Leu Pro Glu Val 
    530                 535                 540                 


Pro Asp Gln Phe Ser Trp Asn Asn Phe Leu Trp Lys Pro Trp Leu Gln 
545                 550                 555                 560 


Leu Cys Cys Pro Ser Thr Gly Leu Ser Cys Gln Cys Asp Arg Glu Lys 
                565                 570                 575     


Gln Asp Lys Arg Leu Ser Arg 
            580             


<210>  2
<211>  1686
<212>  DNA
<213>  Caenorhabditis elegans

<400>  2
atgtccccgg tgagcctatt actacttctt ctattcccat tgagcacttt tcaacaacga       60

gaagattgca cagtgcagcc accatctcca cgtcatccga acattgtgat tcttatgatt      120

gacgacttgg gttacggtga cattgcgtca tacggtcacc cgactcaaga atacacacaa      180

gtcgatcgga tggcagcaga aggcacccgg tttacacaag catattcagc agatagtatg      240

tgctcaccta gcagagctgg attcattact ggacgattac ctattcgatt aggtatagtt      300

ggaggacgga gagtttttgt tccttatgat attggaggcc ttccaaaaag tgagacaaca      360

atggctgaaa tgcttcaaga agcaggatat gcaactggca tggttggaaa atggcatctt      420

ggaattaatg aaaacaatgc aactgatggt gcacatttgc cttcaaagcg aggatttgag      480

tatgttggag tcaacttgcc gtttaccaac gtctggcagt gcgatacaac aagagaattt      540

tacgacaaag gacctgatcc atcactttgt ttcctctatg atggagatga tatcgttcaa      600

caaccaatga aatttgagca tatgacagaa aatcttgttg gagactggaa aaggtttttg      660

atgacaaggt tggcacaaga tcaacacgag agaccattct tcttctattt ctcatttcca      720

caagtccact caacacagtt tgcaagcaaa cgatttagag gatcatcggt tagaggaatc      780

tatggagact ctatcaatga gatgtcatgg gcagttggag aggttttaga cagtcttgta      840

aatgctggaa ttgctgaaaa tacattggtt attttaatgt ctgatcatgg accacatgtt      900

gaactatgtc taaatggtgg atcaacagct ggattgaaag gaggaaagtc gaatagttat      960

gagggaggat tccgaattcc tttcattgct tggcagccag ggacagtgaa accatcccga     1020

gtatcgcatg aagtcatttc cagtatggac ttgtttccaa ctttccgagg aatgcaagaa     1080

caatgcttat ttgagaaaga agcactccgt tctgacggaa ttgatatctc tgacgaattg     1140

cgtggtgaga gtgaagatgt ggaaggaagc ctcggaaagg ctcgtccgat catctactac     1200

tgtaatactc atctgatggc tattaggatg ggagactaca aagttcacta caaaacatct     1260

ccaatcttct ttaataattc agttgatcca aatcttgatt atttctgtcc aaatggaaaa     1320

ccaaagtctg attggtacgt ttcacaagtc tgtcctgatg agcatctaca aaaacattat     1380

cctccacttg ttttcgatct tattcgtgat ccatatgagc agtatccatt gcaaaatact     1440

gtaaaatcac aagagataag attccaagct atgcagcggc tgtctgaaca caaatcgtcg     1500

ctggtcaaag ttaaaaatgt gctcggaagc tacaataaaa cattgatacc ttgctgcaat     1560

ccgccaagtt gcaaatgtga caaattatca aggcccacag aatttgacga aagtcggcca     1620

gattatgttg gtctggtgcc tgatcttgaa aagaccgaat acgaacttct tcacagattc     1680

ttctaa                                                                1686


<210>  3
<211>  561
<212>  PRT
<213>  Caenorhabditis elegans

<400>  3

Met Ser Pro Val Ser Leu Leu Leu Leu Leu Leu Phe Pro Leu Ser Thr 
1               5                   10                  15      


Phe Gln Gln Arg Glu Asp Cys Thr Val Gln Pro Pro Ser Pro Arg His 
            20                  25                  30          


Pro Asn Ile Val Ile Leu Met Ile Asp Asp Leu Gly Tyr Gly Asp Ile 
        35                  40                  45              


Ala Ser Tyr Gly His Pro Thr Gln Glu Tyr Thr Gln Val Asp Arg Met 
    50                  55                  60                  


Ala Ala Glu Gly Thr Arg Phe Thr Gln Ala Tyr Ser Ala Asp Ser Met 
65                  70                  75                  80  


Cys Ser Pro Ser Arg Ala Gly Phe Ile Thr Gly Arg Leu Pro Ile Arg 
                85                  90                  95      


Leu Gly Ile Val Gly Gly Arg Arg Val Phe Val Pro Tyr Asp Ile Gly 
            100                 105                 110         


Gly Leu Pro Lys Ser Glu Thr Thr Met Ala Glu Met Leu Gln Glu Ala 
        115                 120                 125             


Gly Tyr Ala Thr Gly Met Val Gly Lys Trp His Leu Gly Ile Asn Glu 
    130                 135                 140                 


Asn Asn Ala Thr Asp Gly Ala His Leu Pro Ser Lys Arg Gly Phe Glu 
145                 150                 155                 160 


Tyr Val Gly Val Asn Leu Pro Phe Thr Asn Val Trp Gln Cys Asp Thr 
                165                 170                 175     


Thr Arg Glu Phe Tyr Asp Lys Gly Pro Asp Pro Ser Leu Cys Phe Leu 
            180                 185                 190         


Tyr Asp Gly Asp Asp Ile Val Gln Gln Pro Met Lys Phe Glu His Met 
        195                 200                 205             


Thr Glu Asn Leu Val Gly Asp Trp Lys Arg Phe Leu Met Thr Arg Leu 
    210                 215                 220                 


Ala Gln Asp Gln His Glu Arg Pro Phe Phe Phe Tyr Phe Ser Phe Pro 
225                 230                 235                 240 


Gln Val His Ser Thr Gln Phe Ala Ser Lys Arg Phe Arg Gly Ser Ser 
                245                 250                 255     


Val Arg Gly Ile Tyr Gly Asp Ser Ile Asn Glu Met Ser Trp Ala Val 
            260                 265                 270         


Gly Glu Val Leu Asp Ser Leu Val Asn Ala Gly Ile Ala Glu Asn Thr 
        275                 280                 285             


Leu Val Ile Leu Met Ser Asp His Gly Pro His Val Glu Leu Cys Leu 
    290                 295                 300                 


Asn Gly Gly Ser Thr Ala Gly Leu Lys Gly Gly Lys Ser Asn Ser Tyr 
305                 310                 315                 320 


Glu Gly Gly Phe Arg Ile Pro Phe Ile Ala Trp Gln Pro Gly Thr Val 
                325                 330                 335     


Lys Pro Ser Arg Val Ser His Glu Val Ile Ser Ser Met Asp Leu Phe 
            340                 345                 350         


Pro Thr Phe Arg Gly Met Gln Glu Gln Cys Leu Phe Glu Lys Glu Ala 
        355                 360                 365             


Leu Arg Ser Asp Gly Ile Asp Ile Ser Asp Glu Leu Arg Gly Glu Ser 
    370                 375                 380                 


Glu Asp Val Glu Gly Ser Leu Gly Lys Ala Arg Pro Ile Ile Tyr Tyr 
385                 390                 395                 400 


Cys Asn Thr His Leu Met Ala Ile Arg Met Gly Asp Tyr Lys Val His 
                405                 410                 415     


Tyr Lys Thr Ser Pro Ile Phe Phe Asn Asn Ser Val Asp Pro Asn Leu 
            420                 425                 430         


Asp Tyr Phe Cys Pro Asn Gly Lys Pro Lys Ser Asp Trp Tyr Val Ser 
        435                 440                 445             


Gln Val Cys Pro Asp Glu His Leu Gln Lys His Tyr Pro Pro Leu Val 
    450                 455                 460                 


Phe Asp Leu Ile Arg Asp Pro Tyr Glu Gln Tyr Pro Leu Gln Asn Thr 
465                 470                 475                 480 


Val Lys Ser Gln Glu Ile Arg Phe Gln Ala Met Gln Arg Leu Ser Glu 
                485                 490                 495     


His Lys Ser Ser Leu Val Lys Val Lys Asn Val Leu Gly Ser Tyr Asn 
            500                 505                 510         


Lys Thr Leu Ile Pro Cys Cys Asn Pro Pro Ser Cys Lys Cys Asp Lys 
        515                 520                 525             


Leu Ser Arg Pro Thr Glu Phe Asp Glu Ser Arg Pro Asp Tyr Val Gly 
    530                 535                 540                 


Leu Val Pro Asp Leu Glu Lys Thr Glu Tyr Glu Leu Leu His Arg Phe 
545                 550                 555                 560 


Phe 
    


<210>  4
<211>  561
<212>  PRT
<213>  Secuencia Artificial

<220>
<223>  Mutante Sul-2 (pv17)

<400>  4

Met Ser Pro Val Ser Leu Leu Leu Leu Leu Leu Phe Pro Leu Ser Thr 
1               5                   10                  15      


Phe Gln Gln Arg Glu Asp Cys Thr Val Gln Pro Pro Ser Pro Arg His 
            20                  25                  30          


Pro Asn Ile Val Ile Leu Met Ile Asp Asp Leu Gly Tyr Asp Asp Ile 
        35                  40                  45              


Ala Ser Tyr Gly His Pro Thr Gln Glu Tyr Thr Gln Val Asp Arg Met 
    50                  55                  60                  


Ala Ala Glu Gly Thr Arg Phe Thr Gln Ala Tyr Ser Ala Asp Ser Met 
65                  70                  75                  80  


Cys Ser Pro Ser Arg Ala Gly Phe Ile Thr Gly Arg Leu Pro Ile Arg 
                85                  90                  95      


Leu Gly Ile Val Gly Gly Arg Arg Val Phe Val Pro Tyr Asp Ile Gly 
            100                 105                 110         


Gly Leu Pro Lys Ser Glu Thr Thr Met Ala Glu Met Leu Gln Glu Ala 
        115                 120                 125             


Gly Tyr Ala Thr Gly Met Val Gly Lys Trp His Leu Gly Ile Asn Glu 
    130                 135                 140                 


Asn Asn Ala Thr Asp Gly Ala His Leu Pro Ser Lys Arg Gly Phe Glu 
145                 150                 155                 160 


Tyr Val Gly Val Asn Leu Pro Phe Thr Asn Val Trp Gln Cys Asp Thr 
                165                 170                 175     


Thr Arg Glu Phe Tyr Asp Lys Gly Pro Asp Pro Ser Leu Cys Phe Leu 
            180                 185                 190         


Tyr Asp Gly Asp Asp Ile Val Gln Gln Pro Met Lys Phe Glu His Met 
        195                 200                 205             


Thr Glu Asn Leu Val Gly Asp Trp Lys Arg Phe Leu Met Thr Arg Leu 
    210                 215                 220                 


Ala Gln Asp Gln His Glu Arg Pro Phe Phe Phe Tyr Phe Ser Phe Pro 
225                 230                 235                 240 


Gln Val His Ser Thr Gln Phe Ala Ser Lys Arg Phe Arg Gly Ser Ser 
                245                 250                 255     


Val Arg Gly Ile Tyr Gly Asp Ser Ile Asn Glu Met Ser Trp Ala Val 
            260                 265                 270         


Gly Glu Val Leu Asp Ser Leu Val Asn Ala Gly Ile Ala Glu Asn Thr 
        275                 280                 285             


Leu Val Ile Leu Met Ser Asp His Gly Pro His Val Glu Leu Cys Leu 
    290                 295                 300                 


Asn Gly Gly Ser Thr Ala Gly Leu Lys Gly Gly Lys Ser Asn Ser Tyr 
305                 310                 315                 320 


Glu Gly Gly Phe Arg Ile Pro Phe Ile Ala Trp Gln Pro Gly Thr Val 
                325                 330                 335     


Lys Pro Ser Arg Val Ser His Glu Val Ile Ser Ser Met Asp Leu Phe 
            340                 345                 350         


Pro Thr Phe Arg Gly Met Gln Glu Gln Cys Leu Phe Glu Lys Glu Ala 
        355                 360                 365             


Leu Arg Ser Asp Gly Ile Asp Ile Ser Asp Glu Leu Arg Gly Glu Ser 
    370                 375                 380                 


Glu Asp Val Glu Gly Ser Leu Gly Lys Ala Arg Pro Ile Ile Tyr Tyr 
385                 390                 395                 400 


Cys Asn Thr His Leu Met Ala Ile Arg Met Gly Asp Tyr Lys Val His 
                405                 410                 415     


Tyr Lys Thr Ser Pro Ile Phe Phe Asn Asn Ser Val Asp Pro Asn Leu 
            420                 425                 430         


Asp Tyr Phe Cys Pro Asn Gly Lys Pro Lys Ser Asp Trp Tyr Val Ser 
        435                 440                 445             


Gln Val Cys Pro Asp Glu His Leu Gln Lys His Tyr Pro Pro Leu Val 
    450                 455                 460                 


Phe Asp Leu Ile Arg Asp Pro Tyr Glu Gln Tyr Pro Leu Gln Asn Thr 
465                 470                 475                 480 


Val Lys Ser Gln Glu Ile Arg Phe Gln Ala Met Gln Arg Leu Ser Glu 
                485                 490                 495     


His Lys Ser Ser Leu Val Lys Val Lys Asn Val Leu Gly Ser Tyr Asn 
            500                 505                 510         


Lys Thr Leu Ile Pro Cys Cys Asn Pro Pro Ser Cys Lys Cys Asp Lys 
        515                 520                 525             


Leu Ser Arg Pro Thr Glu Phe Asp Glu Ser Arg Pro Asp Tyr Val Gly 
    530                 535                 540                 


Leu Val Pro Asp Leu Glu Lys Thr Glu Tyr Glu Leu Leu His Arg Phe 
545                 550                 555                 560 


Phe 
    


<210>  5
<211>  430
<212>  PRT
<213>  Secuencia Artificial

<220>
<223>  Mutante Sul-2 (gk187)

<400>  5

Met Ser Pro Val Gly Lys Trp His Leu Gly Ile Asn Glu Asn Asn Ala 
1               5                   10                  15      


Thr Asp Gly Ala His Leu Pro Ser Lys Arg Gly Phe Glu Tyr Val Gly 
            20                  25                  30          


Val Asn Leu Pro Phe Thr Asn Val Trp Gln Cys Asp Thr Thr Arg Glu 
        35                  40                  45              


Phe Tyr Asp Lys Gly Pro Asp Pro Ser Leu Cys Phe Leu Tyr Asp Gly 
    50                  55                  60                  


Asp Asp Ile Val Gln Gln Pro Met Lys Phe Glu His Met Thr Glu Asn 
65                  70                  75                  80  


Leu Val Gly Asp Trp Lys Arg Phe Leu Met Thr Arg Leu Ala Gln Asp 
                85                  90                  95      


Gln His Glu Arg Pro Phe Phe Phe Tyr Phe Ser Phe Pro Gln Val His 
            100                 105                 110         


Ser Thr Gln Phe Ala Ser Lys Arg Phe Arg Gly Ser Ser Val Arg Gly 
        115                 120                 125             


Ile Tyr Gly Asp Ser Ile Asn Glu Met Ser Trp Ala Val Gly Glu Val 
    130                 135                 140                 


Leu Asp Ser Leu Val Asn Ala Gly Ile Ala Glu Asn Thr Leu Val Ile 
145                 150                 155                 160 


Leu Met Ser Asp His Gly Pro His Val Glu Leu Cys Leu Asn Gly Gly 
                165                 170                 175     


Ser Thr Ala Gly Leu Lys Gly Gly Lys Ser Asn Ser Tyr Glu Gly Gly 
            180                 185                 190         


Phe Arg Ile Pro Phe Ile Ala Trp Gln Pro Gly Thr Val Lys Pro Ser 
        195                 200                 205             


Arg Val Ser His Glu Val Ile Ser Ser Met Asp Leu Phe Pro Thr Phe 
    210                 215                 220                 


Arg Gly Met Gln Glu Gln Cys Leu Phe Glu Lys Glu Ala Leu Arg Ser 
225                 230                 235                 240 


Asp Gly Ile Asp Ile Ser Asp Glu Leu Arg Gly Glu Ser Glu Asp Val 
                245                 250                 255     


Glu Gly Ser Leu Gly Lys Ala Arg Pro Ile Ile Tyr Tyr Cys Asn Thr 
            260                 265                 270         


His Leu Met Ala Ile Arg Met Gly Asp Tyr Lys Val His Tyr Lys Thr 
        275                 280                 285             


Ser Pro Ile Phe Phe Asn Asn Ser Val Asp Pro Asn Leu Asp Tyr Phe 
    290                 295                 300                 


Cys Pro Asn Gly Lys Pro Lys Ser Asp Trp Tyr Val Ser Gln Val Cys 
305                 310                 315                 320 


Pro Asp Glu His Leu Gln Lys His Tyr Pro Pro Leu Val Phe Asp Leu 
                325                 330                 335     


Ile Arg Asp Pro Tyr Glu Gln Tyr Pro Leu Gln Asn Thr Val Lys Ser 
            340                 345                 350         


Gln Glu Ile Arg Phe Gln Ala Met Gln Arg Leu Ser Glu His Lys Ser 
        355                 360                 365             


Ser Leu Val Lys Val Lys Asn Val Leu Gly Ser Tyr Asn Lys Thr Leu 
    370                 375                 380                 


Ile Pro Cys Cys Asn Pro Pro Ser Cys Lys Cys Asp Lys Leu Ser Arg 
385                 390                 395                 400 


Pro Thr Glu Phe Asp Glu Ser Arg Pro Asp Tyr Val Gly Leu Val Pro 
                405                 410                 415     


Asp Leu Glu Lys Thr Glu Tyr Glu Leu Leu His Arg Phe Phe 
            420                 425                 430 


