                               SEQUENCE LISTING

<110> RUSH UNIVERSITY MEDICAL CENTER
 
<120> PSEUDOMONAS EXOTOXINS FOR CANCER TREATMENT

<130> 006093-0449

<140> PCT/US2014/022499
<141> 2014-03-10

<150> 61/792,606
<151> 2013-03-15

<160> 6     

<170> PatentIn version 3.5

<210> 1
<211> 2064
<212> DNA
<213> Pseudomonas aeruginosa

<400> 1
atgcatatcc aatcgttggg ggctactgcc tcctcgctga atcaggagcc tgtcgaaacc       60

ccgtcgcagg cagcgcataa gtccgccagc ttgcgtcagg aaccttcagg gcaaggtctc      120

ggggttgccc taaagagcac gccgggaata ctttccggga agttgccgga aagcgttagc      180

gacgtgcgtt tcagcagtcc ccaagggcaa ggggagtccc gtactctgac tgactcggca      240

gggccgcggc agatcactct gcgccagttt gagaacggag tcaccgagct acagctcagt      300

cggccaccat tgaccagtct ggtcctaagc ggcggtggtg ccaaaggtgc ggcatacccg      360

ggagcaatgc tggcgctaga agagaaaggc atgctcgatg gcatccgcag catgtccggt      420

tcgtccgctg gcggcatcac cgccgccctt ttggcctcag gtatgagccc ggcggcgttc      480

aagacccttt ccgacaagat ggatcttatt tcgctgctcg acagctcgaa caagaagctg      540

aagctgttcc aacacattag cagcgagatc ggcgcatcgc tgaaaaaggg cttgggcaac      600

aagatcggcg gcttctctga gttgctgctc aatgtactcc cacgcataga ttcgcgggct      660

gagcccctag aacgcctatt gcgcgacgag acacgcaagg ccgtgctcgg acagatcgct      720

acgcatccag aggttgcacg ccagccgacc gttgccgcca tcgccagcag attgcagtcc      780

ggctccggag tcacctttgg cgatctagat cggttgagtg cttacattcc ccagattaag      840

acgctgaaca tcacaggtac ggccatgttc gaggggcgtc cgcaattagt ggtgttcaat      900

gccagccaca caccggatct ggaggtcgcc caggcggcac atatctccgg ttccttccca      960

ggagtgttcc agaaggtcag cttgagtgat cagccgtacc aggccggcgt agagtggaca     1020

gaattccagg atggcggggt gatgattaac gtgccggtcc ctgagatgat cgacaagaat     1080

tttgacagcg ggccactgcg gcgcaacgac aacctgatcc ttgagttcga gggcgaagct     1140

ggggaggtag cgcccgaccg aggtactagg ggcggcgcgc tcaagggctg ggtcgtcggg     1200

gtgcctgccc tgcaggcgcg cgaaatgctg cagctcgagg gcctggagga attgcgcgag     1260

caaaccgttg tggtgccgtt gaagagcgag cgcggtgatt tcagtggcat gctcggtggc     1320

accttgaact tcaccatgcc ggacgagatc aaggcgcatc ttcaggagcg cctccaggag     1380

cgagtcggtg aacatctgga gaaacgtctt caggcttcag agcgtcatac cttcgcttct     1440

ctcgacgagg cgctgctggc acttgatgac agtatgctca ccagtgttgc tcaacagaac     1500

ccggagatca cagacggggc ggtggctttt cgccagaagg cgcgggatgc gttcaccgag     1560

ctgactgtcg ctatcgttag cgccaatggc ttggcgggta ggctcaagtt ggacgaggct     1620

atgcgctccg ctcttcagcg actcgatgcg ctggcagata ctccggaacg cctagcatgg     1680

ttggcagctg agttgaacca tgctgataac gttgatcatc agcagttact cgatgccatg     1740

cgcgggcaga cggtgcagtc gccggtgctc gccgctgcgt tagcagaggc gcagcgccgc     1800

aaagtggcgg ttattgccga gaacattcgt aaggaagtta tcttcccctc tctgtatcgc     1860

cctggccagc cggattccaa cgtagctctg ttacgtcggg cggaggagca gctacggcat     1920

gccaccagtc cggcggaaat caatcaagcg ctgaacgata tcgtcgacaa ctactcggca     1980

cgaggcttcc tgcgtttcgg caaacccttg agttcgacta ccgttgagat ggctaaggct     2040

tggcggaata aggagttcac atga                                            2064


<210> 2
<211> 687
<212> PRT
<213> Pseudomonas aeruginosa

<400> 2
Met His Ile Gln Ser Leu Gly Ala Thr Ala Ser Ser Leu Asn Gln Glu 
1               5                   10                  15      


Pro Val Glu Thr Pro Ser Gln Ala Ala His Lys Ser Ala Ser Leu Arg 
            20                  25                  30          


Gln Glu Pro Ser Gly Gln Gly Leu Gly Val Ala Leu Lys Ser Thr Pro 
        35                  40                  45              


Gly Ile Leu Ser Gly Lys Leu Pro Glu Ser Val Ser Asp Val Arg Phe 
    50                  55                  60                  


Ser Ser Pro Gln Gly Gln Gly Glu Ser Arg Thr Leu Thr Asp Ser Ala 
65                  70                  75                  80  


Gly Pro Arg Gln Ile Thr Leu Arg Gln Phe Glu Asn Gly Val Thr Glu 
                85                  90                  95      


Leu Gln Leu Ser Arg Pro Pro Leu Thr Ser Leu Val Leu Ser Gly Gly 
            100                 105                 110         


Gly Ala Lys Gly Ala Ala Tyr Pro Gly Ala Met Leu Ala Leu Glu Glu 
        115                 120                 125             


Lys Gly Met Leu Asp Gly Ile Arg Ser Met Ser Gly Ser Ser Ala Gly 
    130                 135                 140                 


Gly Ile Thr Ala Ala Leu Leu Ala Ser Gly Met Ser Pro Ala Ala Phe 
145                 150                 155                 160 


Lys Thr Leu Ser Asp Lys Met Asp Leu Ile Ser Leu Leu Asp Ser Ser 
                165                 170                 175     


Asn Lys Lys Leu Lys Leu Phe Gln His Ile Ser Ser Glu Ile Gly Ala 
            180                 185                 190         


Ser Leu Lys Lys Gly Leu Gly Asn Lys Ile Gly Gly Phe Ser Glu Leu 
        195                 200                 205             


Leu Leu Asn Val Leu Pro Arg Ile Asp Ser Arg Ala Glu Pro Leu Glu 
    210                 215                 220                 


Arg Leu Leu Arg Asp Glu Thr Arg Lys Ala Val Leu Gly Gln Ile Ala 
225                 230                 235                 240 


Thr His Pro Glu Val Ala Arg Gln Pro Thr Val Ala Ala Ile Ala Ser 
                245                 250                 255     


Arg Leu Gln Ser Gly Ser Gly Val Thr Phe Gly Asp Leu Asp Arg Leu 
            260                 265                 270         


Ser Ala Tyr Ile Pro Gln Ile Lys Thr Leu Asn Ile Thr Gly Thr Ala 
        275                 280                 285             


Met Phe Glu Gly Arg Pro Gln Leu Val Val Phe Asn Ala Ser His Thr 
    290                 295                 300                 


Pro Asp Leu Glu Val Ala Gln Ala Ala His Ile Ser Gly Ser Phe Pro 
305                 310                 315                 320 


Gly Val Phe Gln Lys Val Ser Leu Ser Asp Gln Pro Tyr Gln Ala Gly 
                325                 330                 335     


Val Glu Trp Thr Glu Phe Gln Asp Gly Gly Val Met Ile Asn Val Pro 
            340                 345                 350         


Val Pro Glu Met Ile Asp Lys Asn Phe Asp Ser Gly Pro Leu Arg Arg 
        355                 360                 365             


Asn Asp Asn Leu Ile Leu Glu Phe Glu Gly Glu Ala Gly Glu Val Ala 
    370                 375                 380                 


Pro Asp Arg Gly Thr Arg Gly Gly Ala Leu Lys Gly Trp Val Val Gly 
385                 390                 395                 400 


Val Pro Ala Leu Gln Ala Arg Glu Met Leu Gln Leu Glu Gly Leu Glu 
                405                 410                 415     


Glu Leu Arg Glu Gln Thr Val Val Val Pro Leu Lys Ser Glu Arg Gly 
            420                 425                 430         


Asp Phe Ser Gly Met Leu Gly Gly Thr Leu Asn Phe Thr Met Pro Asp 
        435                 440                 445             


Glu Ile Lys Ala His Leu Gln Glu Arg Leu Gln Glu Arg Val Gly Glu 
    450                 455                 460                 


His Leu Glu Lys Arg Leu Gln Ala Ser Glu Arg His Thr Phe Ala Ser 
465                 470                 475                 480 


Leu Asp Glu Ala Leu Leu Ala Leu Asp Asp Ser Met Leu Thr Ser Val 
                485                 490                 495     


Ala Gln Gln Asn Pro Glu Ile Thr Asp Gly Ala Val Ala Phe Arg Gln 
            500                 505                 510         


Lys Ala Arg Asp Ala Phe Thr Glu Leu Thr Val Ala Ile Val Ser Ala 
        515                 520                 525             


Asn Gly Leu Ala Gly Arg Leu Lys Leu Asp Glu Ala Met Arg Ser Ala 
    530                 535                 540                 


Leu Gln Arg Leu Asp Ala Leu Ala Asp Thr Pro Glu Arg Leu Ala Trp 
545                 550                 555                 560 


Leu Ala Ala Glu Leu Asn His Ala Asp Asn Val Asp His Gln Gln Leu 
                565                 570                 575     


Leu Asp Ala Met Arg Gly Gln Thr Val Gln Ser Pro Val Leu Ala Ala 
            580                 585                 590         


Ala Leu Ala Glu Ala Gln Arg Arg Lys Val Ala Val Ile Ala Glu Asn 
        595                 600                 605             


Ile Arg Lys Glu Val Ile Phe Pro Ser Leu Tyr Arg Pro Gly Gln Pro 
    610                 615                 620                 


Asp Ser Asn Val Ala Leu Leu Arg Arg Ala Glu Glu Gln Leu Arg His 
625                 630                 635                 640 


Ala Thr Ser Pro Ala Glu Ile Asn Gln Ala Leu Asn Asp Ile Val Asp 
                645                 650                 655     


Asn Tyr Ser Ala Arg Gly Phe Leu Arg Phe Gly Lys Pro Leu Ser Ser 
            660                 665                 670         


Thr Thr Val Glu Met Ala Lys Ala Trp Arg Asn Lys Glu Phe Thr 
        675                 680                 685         


<210> 3
<211> 1374
<212> DNA
<213> Pseudomonas aeruginosa

<400> 3
atgcatattc aatcatctca gcagaacccg tctttcgtgg ctgagttgag ccaggccgtg       60

gccgggcgcc tgggacaggt cgaggcccgc caggtggcca ctccccggga ggcgcaacaa      120

ctggcccagc gccaggaagc accgaagggc gagggcctgc tctcccgcct gggggctgcc      180

ctcgcgcgtc ccttcgtggc gatcatcgag tggctgggca aactgctggg gagccgtgcc      240

cacgccgcca cccaggcgcc gctctcccgt caggacgcgc cgcctgccgc cagtctctct      300

gccgccgaga tcaagcagat gatgctgcaa aaggcactgc ccctgacctt gggcggactt      360

ggcaaggcga gcgagctggc gactttgaca gcggagaggc tggcgaagga tcacacgcgc      420

ctggccagcg gcgacggcgc tctgcgatcg ctggccaccg ccctggtcgg gattcgcgat      480

ggcagccgga tcgaggcttc ccgtacccag gctgcccgcc tgctcgaaca gagcgttggg      540

gggatcgcgc tgcaacagtg ggggaccgcg ggcggtgccg ccagccagca tgtactcagc      600

gcaagcccgg agcaactgcg cgaaatcgcc gtccaactgc atgcggtaat ggacaaggtc      660

gccctgttgc gccacgcggt agagagcgag gtaaagggcg agcctgtcga caaggcgctg      720

gcggatggcc tggtggagca cttcgggctg gaggcggagc agtacctcgg cgaacacccg      780

gacgggccgt acagcgatgc cgaggtgatg gcgctcggtc tctataccaa cggcgagtac      840

cagcacctga atcggtccct gcgtcaggga cgggagctgg atgcgggcca ggcgttgatc      900

gaccggggca tgtctgccgc gttcgaaaag agcggaccgg ctgaacaggt cgtgaagacc      960

ttccgcggca cccagggcag ggatgccttc gaggcggtga aagagggcca ggtcggccac     1020

gacgccggct atctctccac ctcccgggac cccggcgttg ccaggagctt cgcgggccag     1080

ggcacgataa ccaccctgtt cggcagatcc gggatcgatg tcagcgagat atcgatcgag     1140

ggcgatgagc aggagatcct ctacgacaag gggaccgaca tgcgcgtgct tctcagtgcc     1200

aaggatgggc agggtgtgac ccgtcgggtg ctcgaagagg ccacgctggg ggaacggagc     1260

ggccacggcg agggactgct cgatgccctg gacctggcaa ccgggacgga tcgttcaggc     1320

aagccccagg aacaggacct gcgcctgaga atgcgcggcc tcgacctggc ctga           1374


<210> 4
<211> 457
<212> PRT
<213> Pseudomonas aeruginosa

<400> 4
Met His Ile Gln Ser Ser Gln Gln Asn Pro Ser Phe Val Ala Glu Leu 
1               5                   10                  15      


Ser Gln Ala Val Ala Gly Arg Leu Gly Gln Val Glu Ala Arg Gln Val 
            20                  25                  30          


Ala Thr Pro Arg Glu Ala Gln Gln Leu Ala Gln Arg Gln Glu Ala Pro 
        35                  40                  45              


Lys Gly Glu Gly Leu Leu Ser Arg Leu Gly Ala Ala Leu Ala Arg Pro 
    50                  55                  60                  


Phe Val Ala Ile Ile Glu Trp Leu Gly Lys Leu Leu Gly Ser Arg Ala 
65                  70                  75                  80  


His Ala Ala Thr Gln Ala Pro Leu Ser Arg Gln Asp Ala Pro Pro Ala 
                85                  90                  95      


Ala Ser Leu Ser Ala Ala Glu Ile Lys Gln Met Met Leu Gln Lys Ala 
            100                 105                 110         


Leu Pro Leu Thr Leu Gly Gly Leu Gly Lys Ala Ser Glu Leu Ala Thr 
        115                 120                 125             


Leu Thr Ala Glu Arg Leu Ala Lys Asp His Thr Arg Leu Ala Ser Gly 
    130                 135                 140                 


Asp Gly Ala Leu Arg Ser Leu Ala Thr Ala Leu Val Gly Ile Arg Asp 
145                 150                 155                 160 


Gly Ser Arg Ile Glu Ala Ser Arg Thr Gln Ala Ala Arg Leu Leu Glu 
                165                 170                 175     


Gln Ser Val Gly Gly Ile Ala Leu Gln Gln Trp Gly Thr Ala Gly Gly 
            180                 185                 190         


Ala Ala Ser Gln His Val Leu Ser Ala Ser Pro Glu Gln Leu Arg Glu 
        195                 200                 205             


Ile Ala Val Gln Leu His Ala Val Met Asp Lys Val Ala Leu Leu Arg 
    210                 215                 220                 


His Ala Val Glu Ser Glu Val Lys Gly Glu Pro Val Asp Lys Ala Leu 
225                 230                 235                 240 


Ala Asp Gly Leu Val Glu His Phe Gly Leu Glu Ala Glu Gln Tyr Leu 
                245                 250                 255     


Gly Glu His Pro Asp Gly Pro Tyr Ser Asp Ala Glu Val Met Ala Leu 
            260                 265                 270         


Gly Leu Tyr Thr Asn Gly Glu Tyr Gln His Leu Asn Arg Ser Leu Arg 
        275                 280                 285             


Gln Gly Arg Glu Leu Asp Ala Gly Gln Ala Leu Ile Asp Arg Gly Met 
    290                 295                 300                 


Ser Ala Ala Phe Glu Lys Ser Gly Pro Ala Glu Gln Val Val Lys Thr 
305                 310                 315                 320 


Phe Arg Gly Thr Gln Gly Arg Asp Ala Phe Glu Ala Val Lys Glu Gly 
                325                 330                 335     


Gln Val Gly His Asp Ala Gly Tyr Leu Ser Thr Ser Arg Asp Pro Gly 
            340                 345                 350         


Val Ala Arg Ser Phe Ala Gly Gln Gly Thr Ile Thr Thr Leu Phe Gly 
        355                 360                 365             


Arg Ser Gly Ile Asp Val Ser Glu Ile Ser Ile Glu Gly Asp Glu Gln 
    370                 375                 380                 


Glu Ile Leu Tyr Asp Lys Gly Thr Asp Met Arg Val Leu Leu Ser Ala 
385                 390                 395                 400 


Lys Asp Gly Gln Gly Val Thr Arg Arg Val Leu Glu Glu Ala Thr Leu 
                405                 410                 415     


Gly Glu Arg Ser Gly His Gly Glu Gly Leu Leu Asp Ala Leu Asp Leu 
            420                 425                 430         


Ala Thr Gly Thr Asp Arg Ser Gly Lys Pro Gln Glu Gln Asp Leu Arg 
        435                 440                 445             


Leu Arg Met Arg Gly Leu Asp Leu Ala 
    450                 455         


<210> 5
<211> 1362
<212> DNA
<213> Pseudomonas aeruginosa

<400> 5
atgcatattc aatcgctcca gcagagtccg tctttcgccg tcgaattgca ccaggccgcc       60

agtgggcgtt tgggacagat tgaggcccgc caggtcgcca cccccagtga agcgcagcag      120

ttggcccagc gccaggacgc gccgaagggt gaggggctgc tcgctcgcct gggcgcggcg      180

ctcgtgcgtc cgttcgtggc gatcatggac tggctgggca aactgttggg ctcccacgcc      240

cgcaccgggc cgcagcccag tcaggacgcg cagcctgcgg tcatgtcctc ggccgtcgtg      300

ttcaagcaga tggtgctgca gcaggcattg cccatgacct tgaagggact cgacaaggcg      360

agcgagctgg cgaccctgac accggaagga ctggcccggg agcactcccg cctggccagc      420

ggagatgggg cgctgcgttc gctgagcacc gccttggccg gcattcgtgc cggcagccag      480

gtcgaggagt cccgtatcca ggctggccgc ctgctcgaac ggagcatcgg cgggatcgcg      540

ctgcagcagt ggggcaccac cggcggtgcc gcgagtcaac tggtgctcga cgcaagcccg      600

gaactgcggc gcgaaatcac cgaccagttg catcaggtaa tgagcgaggt cgcactgttg      660

cgccaagcgg tagagagcga ggtcagcaga gtatcggccg acaaggcgct ggcggatggc      720

ctggtgaagc ggttcggggc ggatgcggaa aagtacctgg gcagacagcc tggtggcatc      780

cacagtgacg ccgaagtgat ggcgcttggt ctctacaccg gcattcacta cgcggacctg      840

aatcgcgctc tgcgtcaggg gcaggagctg gatgcgggac aaaagctgat cgaccaaggt      900

atgtccgcgg ccttcgagaa gagcggacag gctgaacagg tagtgaagac tttccgtggc      960

acccgtggcg gggatgcctt caacgcagtg gaagagggca aggttggcca cgacgacggc     1020

tatctctcca cctccctgaa ccccggtgtc gcgaggagct tcgggcaggg cacgatatcc     1080

accgtgttcg gcaggtccgg aatcgatgtc agcgggatat cgaactacaa gaatgaaaaa     1140

gagattctct ataacaaaga aaccgacatg cgcgtgctgc tgagcgccag cgatgagcag     1200

ggagtgaccc gccgggttct cgaagaggcg gccctggggg agcagagtgg ccatagccag     1260

ggactgctcg atgctctcga cctggcaagc aaaccggaac gttcaggcga ggtccaggaa     1320

caggatgtac gcctgaggat gcgcggcctt gatctggcct ga                        1362


<210> 6
<211> 453
<212> PRT
<213> Pseudomonas aeruginosa

<400> 6
Met His Ile Gln Ser Leu Gln Gln Ser Pro Ser Phe Ala Val Glu Leu 
1               5                   10                  15      


His Gln Ala Ala Ser Gly Arg Leu Gly Gln Ile Glu Ala Arg Gln Val 
            20                  25                  30          


Ala Thr Pro Ser Glu Ala Gln Gln Leu Ala Gln Arg Gln Asp Ala Pro 
        35                  40                  45              


Lys Gly Glu Gly Leu Leu Ala Arg Leu Gly Ala Ala Leu Val Arg Pro 
    50                  55                  60                  


Phe Val Ala Ile Met Asp Trp Leu Gly Lys Leu Leu Gly Ser His Ala 
65                  70                  75                  80  


Arg Thr Gly Pro Gln Pro Ser Gln Asp Ala Gln Pro Ala Val Met Ser 
                85                  90                  95      


Ser Ala Val Val Phe Lys Gln Met Val Leu Gln Gln Ala Leu Pro Met 
            100                 105                 110         


Thr Leu Lys Gly Leu Asp Lys Ala Ser Glu Leu Ala Thr Leu Thr Pro 
        115                 120                 125             


Glu Gly Leu Ala Arg Glu His Ser Arg Leu Ala Ser Gly Asp Gly Ala 
    130                 135                 140                 


Leu Arg Ser Leu Ser Thr Ala Leu Ala Gly Ile Arg Ala Gly Ser Gln 
145                 150                 155                 160 


Val Glu Glu Ser Arg Ile Gln Ala Gly Arg Leu Leu Glu Arg Ser Ile 
                165                 170                 175     


Gly Gly Ile Ala Leu Gln Gln Trp Gly Thr Thr Gly Gly Ala Ala Ser 
            180                 185                 190         


Gln Leu Val Leu Asp Ala Ser Pro Glu Leu Arg Arg Glu Ile Thr Asp 
        195                 200                 205             


Gln Leu His Gln Val Met Ser Glu Val Ala Leu Leu Arg Gln Ala Val 
    210                 215                 220                 


Glu Ser Glu Val Ser Arg Val Ser Ala Asp Lys Ala Leu Ala Asp Gly 
225                 230                 235                 240 


Leu Val Lys Arg Phe Gly Ala Asp Ala Glu Lys Tyr Leu Gly Arg Gln 
                245                 250                 255     


Pro Gly Gly Ile His Ser Asp Ala Glu Val Met Ala Leu Gly Leu Tyr 
            260                 265                 270         


Thr Gly Ile His Tyr Ala Asp Leu Asn Arg Ala Leu Arg Gln Gly Gln 
        275                 280                 285             


Glu Leu Asp Ala Gly Gln Lys Leu Ile Asp Gln Gly Met Ser Ala Ala 
    290                 295                 300                 


Phe Glu Lys Ser Gly Gln Ala Glu Gln Val Val Lys Thr Phe Arg Gly 
305                 310                 315                 320 


Thr Arg Gly Gly Asp Ala Phe Asn Ala Val Glu Glu Gly Lys Val Gly 
                325                 330                 335     


His Asp Asp Gly Tyr Leu Ser Thr Ser Leu Asn Pro Gly Val Ala Arg 
            340                 345                 350         


Ser Phe Gly Gln Gly Thr Ile Ser Thr Val Phe Gly Arg Ser Gly Ile 
        355                 360                 365             


Asp Val Ser Gly Ile Ser Asn Tyr Lys Asn Glu Lys Glu Ile Leu Tyr 
    370                 375                 380                 


Asn Lys Glu Thr Asp Met Arg Val Leu Leu Ser Ala Ser Asp Glu Gln 
385                 390                 395                 400 


Gly Val Thr Arg Arg Val Leu Glu Glu Ala Ala Leu Gly Glu Gln Ser 
                405                 410                 415     


Gly His Ser Gln Gly Leu Leu Asp Ala Leu Asp Leu Ala Ser Lys Pro 
            420                 425                 430         


Glu Arg Ser Gly Glu Val Gln Glu Gln Asp Val Arg Leu Arg Met Arg 
        435                 440                 445             


Gly Leu Asp Leu Ala 
    450             


