                         SEQUENCE LISTING

<110>  Massare, Michael
 
<120>  Enhanced Expression of Proteins

<130>  NOVV-052/00US 305047 2363

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  2316
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BV1169 plasmid encoding FMDV protein P1 (VP1-4), HA signal 
       peptide, and His6 tag

<400>  1
atggaaaaga tcgtgctcct cctcgctatc gtgtccctgg tcaagatggg tgccggtcaa       60

tcgtcgccag ctacaggctc gcaaaaccag tcgggtaaca caggctctat catcaacaac      120

tactacatgc aacagtacca aaacagcatg gacacccagc tgggagataa cgctatctct      180

ggtggcagca acgagggttc gacagatacc acttccaccc acacaaccaa cactcaaaac      240

aacgactggt tctccaagtt ggcctcctct gctttctctg gcctgttcgg agccctgctc      300

gctgataaga agaccgagga aactacattg ctggaggacc gtatcctgac cactagaaac      360

ggccacacaa cctccactac acagagctca gttggcgtga catacggata cgccaccgct      420

gaagacttcg tgtctggacc caacactagc ggtttggaga cacgtgtggt ccaagctgaa      480

cgcttcttca agacacacct gttcgactgg gtgacctcgg attccttcgg tagatgccac      540

ctcttggagc tccctaccga ccacaagggt gtctacggct ccttgactga ttcttacgcc      600

tacatgagga acggttggga cgtcgaagtt accgctgtgg gcaaccagtt caacggaggt      660

tgcctgctcg tggccatggt ccccgagctc tgttctatcc agaagaggga attgtaccaa      720

ctgaccctct tcccccacca gttcatcaac cctagaacta acatgacagc tcacatcact      780

gtgccattcg tgggcgtcaa ccgttacgac caatacaagg tccacaagcc ctggaccctg      840

gttgtgatgg tcgttgcccc cctcaccgtg aacactgagg gtgctcctca gatcaaggtc      900

tacgccaaca tcgctcccac taacgttcac gtggccggag agttcccaag caaggaaggt      960

atcttcccag tggcttgctc agacggatac ggaggactgg tcaccactga cccaaagacc     1020

gctgatccgg tttacggcaa ggtgttcaac cccccccgta accagctccc tggcagattc     1080

actaacttgc tggacgtggc tgaggcttgt ccaacattcc tgcgcttcga aggtggcgtt     1140

ccgtacgtga caaccaagac cgactcagat agggtcttgg ctcagttcga catgtcactg     1200

gctgccaagc acatgtcgaa cactttcctc gccggattgg ctcagtacta cacccaatac     1260

tccggtacta tcaaccccca cttcatgttc acaggcccta ccgacgccaa ggctcgctac     1320

atgatcgctt acgctccacc gggaatggag ccacctaaga ccccagaagc tgccgctcac     1380

tgcatccacg ccgagtggga cactggtctg aactcaaagt tcacattctc gatcccctac     1440

ctctccgccg ctgactacgc ctacaccgct tctgatgtgg ctgaaactac aaacgtccag     1500

ggttgggttt gtctgttcca aatcactcac ggcaaggctg acggcgatgc tctggtggtc     1560

ctcgctagcg ctggcaagga cttcgagttg cgtctgcctg tggatgcccg cgctgagacc     1620

actagcgctg gcgaatcagc tgacccagtg acaaccactg tcgaggatta cggaggtgaa     1680

actcagatcc aacgtcgcca gcacacagac gtgtctttca tcatggatcg tttcgtcaag     1740

gttaccccac agaaccaaat caacatcctc gacttgatgc aaatcccgag ccacacactg     1800

gtcggagctc tcttgagagc ttctacctac tacttcagcg atttggagat cgccgtcaag     1860

cacgaaggag acctgacctg ggttccaaac ggcgctccgg agaagggatt ggacaacaca     1920

accaacccaa ctgcctacca caaggctccg ctgacccgtc tggctctccc ctacactgct     1980

cctcaccgcg ttctcgctac cgtgtacaac ggagaatgcc gttactcacg caacgccgtc     2040

cccaacgttc gtggtgactt gcaggtgctg gctcaaaagg ttgtgcgcac tctgcctaca     2100

tcgttcaact acggtgccat caaggctacc cgcgtcactg agctgctcta caggatgaag     2160

agagccgaaa cttactgccc caggcctttg ctggccatcc accccacaga ggctagacac     2220

aagcagaaga tcgtggctcc tgtcaagcaa accctcaact tcgacctctt gaagttggcc     2280

ggcgatgtga cagagcacca ccaccaccac cactaa                               2316


<210>  2
<211>  771
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FMDV protein P1 (VP1-4) HA signal peptide and His6 tag

<400>  2

Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Met 
1               5                   10                  15      


Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser Gly 
            20                  25                  30          


Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln Asn 
        35                  40                  45              


Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser Asn 
    50                  55                  60                  


Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Thr Asn Thr Gln Asn 
65                  70                  75                  80  


Asn Asp Trp Phe Ser Lys Leu Ala Ser Ser Ala Phe Ser Gly Leu Phe 
                85                  90                  95      


Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu Glu 
            100                 105                 110         


Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr Gln 
        115                 120                 125             


Ser Ser Val Gly Val Thr Tyr Gly Tyr Ala Thr Ala Glu Asp Phe Val 
    130                 135                 140                 


Ser Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Val Gln Ala Glu 
145                 150                 155                 160 


Arg Phe Phe Lys Thr His Leu Phe Asp Trp Val Thr Ser Asp Ser Phe 
                165                 170                 175     


Gly Arg Cys His Leu Leu Glu Leu Pro Thr Asp His Lys Gly Val Tyr 
            180                 185                 190         


Gly Ser Leu Thr Asp Ser Tyr Ala Tyr Met Arg Asn Gly Trp Asp Val 
        195                 200                 205             


Glu Val Thr Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu Val 
    210                 215                 220                 


Ala Met Val Pro Glu Leu Cys Ser Ile Gln Lys Arg Glu Leu Tyr Gln 
225                 230                 235                 240 


Leu Thr Leu Phe Pro His Gln Phe Ile Asn Pro Arg Thr Asn Met Thr 
                245                 250                 255     


Ala His Ile Thr Val Pro Phe Val Gly Val Asn Arg Tyr Asp Gln Tyr 
            260                 265                 270         


Lys Val His Lys Pro Trp Thr Leu Val Val Met Val Val Ala Pro Leu 
        275                 280                 285             


Thr Val Asn Thr Glu Gly Ala Pro Gln Ile Lys Val Tyr Ala Asn Ile 
    290                 295                 300                 


Ala Pro Thr Asn Val His Val Ala Gly Glu Phe Pro Ser Lys Glu Gly 
305                 310                 315                 320 


Ile Phe Pro Val Ala Cys Ser Asp Gly Tyr Gly Gly Leu Val Thr Thr 
                325                 330                 335     


Asp Pro Lys Thr Ala Asp Pro Val Tyr Gly Lys Val Phe Asn Pro Pro 
            340                 345                 350         


Arg Asn Gln Leu Pro Gly Arg Phe Thr Asn Leu Leu Asp Val Ala Glu 
        355                 360                 365             


Ala Cys Pro Thr Phe Leu Arg Phe Glu Gly Gly Val Pro Tyr Val Thr 
    370                 375                 380                 


Thr Lys Thr Asp Ser Asp Arg Val Leu Ala Gln Phe Asp Met Ser Leu 
385                 390                 395                 400 


Ala Ala Lys His Met Ser Asn Thr Phe Leu Ala Gly Leu Ala Gln Tyr 
                405                 410                 415     


Tyr Thr Gln Tyr Ser Gly Thr Ile Asn Pro His Phe Met Phe Thr Gly 
            420                 425                 430         


Pro Thr Asp Ala Lys Ala Arg Tyr Met Ile Ala Tyr Ala Pro Pro Gly 
        435                 440                 445             


Met Glu Pro Pro Lys Thr Pro Glu Ala Ala Ala His Cys Ile His Ala 
    450                 455                 460                 


Glu Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro Tyr 
465                 470                 475                 480 


Leu Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Val Ala Glu Thr 
                485                 490                 495     


Thr Asn Val Gln Gly Trp Val Cys Leu Phe Gln Ile Thr His Gly Lys 
            500                 505                 510         


Ala Asp Gly Asp Ala Leu Val Val Leu Ala Ser Ala Gly Lys Asp Phe 
        515                 520                 525             


Glu Leu Arg Leu Pro Val Asp Ala Arg Ala Glu Thr Thr Ser Ala Gly 
    530                 535                 540                 


Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu Asp Tyr Gly Gly Glu 
545                 550                 555                 560 


Thr Gln Ile Gln Arg Arg Gln His Thr Asp Val Ser Phe Ile Met Asp 
                565                 570                 575     


Arg Phe Val Lys Val Thr Pro Gln Asn Gln Ile Asn Ile Leu Asp Leu 
            580                 585                 590         


Met Gln Ile Pro Ser His Thr Leu Val Gly Ala Leu Leu Arg Ala Ser 
        595                 600                 605             


Thr Tyr Tyr Phe Ser Asp Leu Glu Ile Ala Val Lys His Glu Gly Asp 
    610                 615                 620                 


Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Lys Gly Leu Asp Asn Thr 
625                 630                 635                 640 


Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu Thr Arg Leu Ala Leu 
                645                 650                 655     


Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr Val Tyr Asn Gly Glu 
            660                 665                 670         


Cys Arg Tyr Ser Arg Asn Ala Val Pro Asn Val Arg Gly Asp Leu Gln 
        675                 680                 685             


Val Leu Ala Gln Lys Val Val Arg Thr Leu Pro Thr Ser Phe Asn Tyr 
    690                 695                 700                 


Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu Leu Tyr Arg Met Lys 
705                 710                 715                 720 


Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu Ala Ile His Pro Thr 
                725                 730                 735     


Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
            740                 745                 750         


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Thr Glu His His His 
        755                 760                 765             


His His His 
    770     


<210>  3
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA signal peptide from A/Indonesia/5/05

<400>  3

Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys 
1               5                   10                  15  


<210>  4
<211>  2271
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BV1168 plasmid encoding FMDV protein P1 (VP1-4) and His6 tag

<400>  4
atgggtgccg gtcaatcgtc gccagctaca ggctcgcaaa accagtcggg taacacaggc       60

tctatcatca acaactacta catgcaacag taccaaaaca gcatggacac ccagctggga      120

gataacgcta tctctggtgg cagcaacgag ggttcgacag ataccacttc cacccacaca      180

accaacactc aaaacaacga ctggttctcc aagttggcct cctctgcttt ctctggcctg      240

ttcggagccc tgctcgctga taagaagacc gaggaaacta cattgctgga ggaccgtatc      300

ctgaccacta gaaacggcca cacaacctcc actacacaga gctcagttgg cgtgacatac      360

ggatacgcca ccgctgaaga cttcgtgtct ggacccaaca ctagcggttt ggagacacgt      420

gtggtccaag ctgaacgctt cttcaagaca cacctgttcg actgggtgac ctcggattcc      480

ttcggtagat gccacctctt ggagctccct accgaccaca agggtgtcta cggctccttg      540

actgattctt acgcctacat gaggaacggt tgggacgtcg aagttaccgc tgtgggcaac      600

cagttcaacg gaggttgcct gctcgtggcc atggtccccg agctctgttc tatccagaag      660

agggaattgt accaactgac cctcttcccc caccagttca tcaaccctag aactaacatg      720

acagctcaca tcactgtgcc attcgtgggc gtcaaccgtt acgaccaata caaggtccac      780

aagccctgga ccctggttgt gatggtcgtt gcccccctca ccgtgaacac tgagggtgct      840

cctcagatca aggtctacgc caacatcgct cccactaacg ttcacgtggc cggagagttc      900

ccaagcaagg aaggtatctt cccagtggct tgctcagacg gatacggagg actggtcacc      960

actgacccaa agaccgctga tccggtttac ggcaaggtgt tcaacccccc ccgtaaccag     1020

ctccctggca gattcactaa cttgctggac gtggctgagg cttgtccaac attcctgcgc     1080

ttcgaaggtg gcgttccgta cgtgacaacc aagaccgact cagatagggt cttggctcag     1140

ttcgacatgt cactggctgc caagcacatg tcgaacactt tcctcgccgg attggctcag     1200

tactacaccc aatactccgg tactatcaac ccccacttca tgttcacagg ccctaccgac     1260

gccaaggctc gctacatgat cgcttacgct ccaccgggaa tggagccacc taagacccca     1320

gaagctgccg ctcactgcat ccacgccgag tgggacactg gtctgaactc aaagttcaca     1380

ttctcgatcc cctacctctc cgccgctgac tacgcctaca ccgcttctga tgtggctgaa     1440

actacaaacg tccagggttg ggtttgtctg ttccaaatca ctcacggcaa ggctgacggc     1500

gatgctctgg tggtcctcgc tagcgctggc aaggacttcg agttgcgtct gcctgtggat     1560

gcccgcgctg agaccactag cgctggcgaa tcagctgacc cagtgacaac cactgtcgag     1620

gattacggag gtgaaactca gatccaacgt cgccagcaca cagacgtgtc tttcatcatg     1680

gatcgtttcg tcaaggttac cccacagaac caaatcaaca tcctcgactt gatgcaaatc     1740

ccgagccaca cactggtcgg agctctcttg agagcttcta cctactactt cagcgatttg     1800

gagatcgccg tcaagcacga aggagacctg acctgggttc caaacggcgc tccggagaag     1860

ggattggaca acacaaccaa cccaactgcc taccacaagg ctccgctgac ccgtctggct     1920

ctcccctaca ctgctcctca ccgcgttctc gctaccgtgt acaacggaga atgccgttac     1980

tcacgcaacg ccgtccccaa cgttcgtggt gacttgcagg tgctggctca aaaggttgtg     2040

cgcactctgc ctacatcgtt caactacggt gccatcaagg ctacccgcgt cactgagctg     2100

ctctacagga tgaagagagc cgaaacttac tgccccaggc ctttgctggc catccacccc     2160

acagaggcta gacacaagca gaagatcgtg gctcctgtca agcaaaccct caacttcgac     2220

ctcttgaagt tggccggcga tgtgacagag caccaccacc accaccacta a              2271


<210>  5
<211>  756
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FMDV protein P1 (VP1-4) and His6 tag

<400>  5

Met Gly Ala Gly Gln Ser Ser Pro Ala Thr Gly Ser Gln Asn Gln Ser 
1               5                   10                  15      


Gly Asn Thr Gly Ser Ile Ile Asn Asn Tyr Tyr Met Gln Gln Tyr Gln 
            20                  25                  30          


Asn Ser Met Asp Thr Gln Leu Gly Asp Asn Ala Ile Ser Gly Gly Ser 
        35                  40                  45              


Asn Glu Gly Ser Thr Asp Thr Thr Ser Thr His Thr Thr Asn Thr Gln 
    50                  55                  60                  


Asn Asn Asp Trp Phe Ser Lys Leu Ala Ser Ser Ala Phe Ser Gly Leu 
65                  70                  75                  80  


Phe Gly Ala Leu Leu Ala Asp Lys Lys Thr Glu Glu Thr Thr Leu Leu 
                85                  90                  95      


Glu Asp Arg Ile Leu Thr Thr Arg Asn Gly His Thr Thr Ser Thr Thr 
            100                 105                 110         


Gln Ser Ser Val Gly Val Thr Tyr Gly Tyr Ala Thr Ala Glu Asp Phe 
        115                 120                 125             


Val Ser Gly Pro Asn Thr Ser Gly Leu Glu Thr Arg Val Val Gln Ala 
    130                 135                 140                 


Glu Arg Phe Phe Lys Thr His Leu Phe Asp Trp Val Thr Ser Asp Ser 
145                 150                 155                 160 


Phe Gly Arg Cys His Leu Leu Glu Leu Pro Thr Asp His Lys Gly Val 
                165                 170                 175     


Tyr Gly Ser Leu Thr Asp Ser Tyr Ala Tyr Met Arg Asn Gly Trp Asp 
            180                 185                 190         


Val Glu Val Thr Ala Val Gly Asn Gln Phe Asn Gly Gly Cys Leu Leu 
        195                 200                 205             


Val Ala Met Val Pro Glu Leu Cys Ser Ile Gln Lys Arg Glu Leu Tyr 
    210                 215                 220                 


Gln Leu Thr Leu Phe Pro His Gln Phe Ile Asn Pro Arg Thr Asn Met 
225                 230                 235                 240 


Thr Ala His Ile Thr Val Pro Phe Val Gly Val Asn Arg Tyr Asp Gln 
                245                 250                 255     


Tyr Lys Val His Lys Pro Trp Thr Leu Val Val Met Val Val Ala Pro 
            260                 265                 270         


Leu Thr Val Asn Thr Glu Gly Ala Pro Gln Ile Lys Val Tyr Ala Asn 
        275                 280                 285             


Ile Ala Pro Thr Asn Val His Val Ala Gly Glu Phe Pro Ser Lys Glu 
    290                 295                 300                 


Gly Ile Phe Pro Val Ala Cys Ser Asp Gly Tyr Gly Gly Leu Val Thr 
305                 310                 315                 320 


Thr Asp Pro Lys Thr Ala Asp Pro Val Tyr Gly Lys Val Phe Asn Pro 
                325                 330                 335     


Pro Arg Asn Gln Leu Pro Gly Arg Phe Thr Asn Leu Leu Asp Val Ala 
            340                 345                 350         


Glu Ala Cys Pro Thr Phe Leu Arg Phe Glu Gly Gly Val Pro Tyr Val 
        355                 360                 365             


Thr Thr Lys Thr Asp Ser Asp Arg Val Leu Ala Gln Phe Asp Met Ser 
    370                 375                 380                 


Leu Ala Ala Lys His Met Ser Asn Thr Phe Leu Ala Gly Leu Ala Gln 
385                 390                 395                 400 


Tyr Tyr Thr Gln Tyr Ser Gly Thr Ile Asn Pro His Phe Met Phe Thr 
                405                 410                 415     


Gly Pro Thr Asp Ala Lys Ala Arg Tyr Met Ile Ala Tyr Ala Pro Pro 
            420                 425                 430         


Gly Met Glu Pro Pro Lys Thr Pro Glu Ala Ala Ala His Cys Ile His 
        435                 440                 445             


Ala Glu Trp Asp Thr Gly Leu Asn Ser Lys Phe Thr Phe Ser Ile Pro 
    450                 455                 460                 


Tyr Leu Ser Ala Ala Asp Tyr Ala Tyr Thr Ala Ser Asp Val Ala Glu 
465                 470                 475                 480 


Thr Thr Asn Val Gln Gly Trp Val Cys Leu Phe Gln Ile Thr His Gly 
                485                 490                 495     


Lys Ala Asp Gly Asp Ala Leu Val Val Leu Ala Ser Ala Gly Lys Asp 
            500                 505                 510         


Phe Glu Leu Arg Leu Pro Val Asp Ala Arg Ala Glu Thr Thr Ser Ala 
        515                 520                 525             


Gly Glu Ser Ala Asp Pro Val Thr Thr Thr Val Glu Asp Tyr Gly Gly 
    530                 535                 540                 


Glu Thr Gln Ile Gln Arg Arg Gln His Thr Asp Val Ser Phe Ile Met 
545                 550                 555                 560 


Asp Arg Phe Val Lys Val Thr Pro Gln Asn Gln Ile Asn Ile Leu Asp 
                565                 570                 575     


Leu Met Gln Ile Pro Ser His Thr Leu Val Gly Ala Leu Leu Arg Ala 
            580                 585                 590         


Ser Thr Tyr Tyr Phe Ser Asp Leu Glu Ile Ala Val Lys His Glu Gly 
        595                 600                 605             


Asp Leu Thr Trp Val Pro Asn Gly Ala Pro Glu Lys Gly Leu Asp Asn 
    610                 615                 620                 


Thr Thr Asn Pro Thr Ala Tyr His Lys Ala Pro Leu Thr Arg Leu Ala 
625                 630                 635                 640 


Leu Pro Tyr Thr Ala Pro His Arg Val Leu Ala Thr Val Tyr Asn Gly 
                645                 650                 655     


Glu Cys Arg Tyr Ser Arg Asn Ala Val Pro Asn Val Arg Gly Asp Leu 
            660                 665                 670         


Gln Val Leu Ala Gln Lys Val Val Arg Thr Leu Pro Thr Ser Phe Asn 
        675                 680                 685             


Tyr Gly Ala Ile Lys Ala Thr Arg Val Thr Glu Leu Leu Tyr Arg Met 
    690                 695                 700                 


Lys Arg Ala Glu Thr Tyr Cys Pro Arg Pro Leu Leu Ala Ile His Pro 
705                 710                 715                 720 


Thr Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr 
                725                 730                 735     


Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Thr Glu His His 
            740                 745                 750         


His His His His 
        755     


<210>  6
<211>  500
<212>  PRT
<213>  Influenza virus

<400>  6

Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser 
1               5                   10                  15      


Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
            20                  25                  30          


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
        35                  40                  45              


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
    50                  55                  60                  


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
65                  70                  75                  80  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
                85                  90                  95      


Glu Lys Ala Asn Pro Thr Asn Asp Leu Cys Tyr Pro Gly Ser Phe Asn 
            100                 105                 110         


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
        115                 120                 125             


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser 
    130                 135                 140                 


Ser Gly Val Ser Ser Ala Cys Pro Tyr Leu Gly Ser Pro Ser Phe Phe 
145                 150                 155                 160 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Ser Thr Tyr Pro Thr Ile 
                165                 170                 175     


Lys Lys Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
            180                 185                 190         


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln 
        195                 200                 205             


Asn Pro Thr Thr Tyr Ile Ser Ile Gly Thr Ser Thr Leu Asn Gln Arg 
    210                 215                 220                 


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly 
225                 230                 235                 240 


Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
                245                 250                 255     


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
            260                 265                 270         


Val Lys Lys Gly Asp Ser Ala Ile Met Lys Ser Glu Leu Glu Tyr Gly 
        275                 280                 285             


Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile Asn Ser Ser 
    290                 295                 300                 


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 


Tyr Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
                325                 330                 335     


Pro Gln Arg Glu Ser Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile 
            340                 345                 350         


Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr 
        355                 360                 365             


Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys 
    370                 375                 380                 


Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser 
385                 390                 395                 400 


Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe 
                405                 410                 415     


Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp 
            420                 425                 430         


Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met 
        435                 440                 445             


Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu 
    450                 455                 460                 


Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly 
465                 470                 475                 480 


Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu 
                485                 490                 495     


Ser Ile Arg Asn 
            500 


