                         SEQUENCE LISTING

<110>  AMERICAN UNIVERSITY OF CAIRO
       SIAM, Rania
       MOHAMED, Yasmine
       EL DORRY, Hamza
 
<120>  ESTERASE RESISTANT TO INACTIVATION BY HEAVY METALS

<130>  413672WO

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  945
<212>  DNA
<213>  Unknown

<220>
<223>  Isolate from Atlantis II Red Sea Brine Pool


<220>
<221>  CDS
<222>  (1)..(945)

<400>  1
atg tcc agg tac gtt gat gag ctg tat cgc aac ccg ggc caa ccc ggc         48
Met Ser Arg Tyr Val Asp Glu Leu Tyr Arg Asn Pro Gly Gln Pro Gly           
1               5                   10                  15                

ctg cgc gcc ctg ctg cgc ggc atg ctg aaa ctg ctg ttc cgg ggt ttg         96
Leu Arg Ala Leu Leu Arg Gly Met Leu Lys Leu Leu Phe Arg Gly Leu           
            20                  25                  30                    

atc cgt ccg ccc gtg ccc ttt gcc gtg cag gca ctg gtg ctg cgc ctg        144
Ile Arg Pro Pro Val Pro Phe Ala Val Gln Ala Leu Val Leu Arg Leu           
        35                  40                  45                        

ctg acc ctc ggc atg ccg ctg gcc aga ggc gtg acc cgc agc acc gag        192
Leu Thr Leu Gly Met Pro Leu Ala Arg Gly Val Thr Arg Ser Thr Glu           
    50                  55                  60                            

cag atc gcc gga cgg ccc tgt atg tgg cac cgc ccg gcc gct ggc ggc        240
Gln Ile Ala Gly Arg Pro Cys Met Trp His Arg Pro Ala Ala Gly Gly           
65                  70                  75                  80            

aac ggc cgc gtg ctg ctg tac ctg cat ggc ggc gcc ttc gtc atc ggc        288
Asn Gly Arg Val Leu Leu Tyr Leu His Gly Gly Ala Phe Val Ile Gly           
                85                  90                  95                

tcc ccg cag acc cac cgc ggc atc tgc tcg gcg ctc gcc agc cgt ggt        336
Ser Pro Gln Thr His Arg Gly Ile Cys Ser Ala Leu Ala Ser Arg Gly           
            100                 105                 110                   

cag ttt gat gtc tgc gca ctc gat tac cga ctg gcg ccg gcg cac ccg        384
Gln Phe Asp Val Cys Ala Leu Asp Tyr Arg Leu Ala Pro Ala His Pro           
        115                 120                 125                       

gca ccg gcg gcc tgt gac gat gcg gtc gcc gcc tat cag gcc ctg ctg        432
Ala Pro Ala Ala Cys Asp Asp Ala Val Ala Ala Tyr Gln Ala Leu Leu           
    130                 135                 140                           

cag cga ggc tat gcg ccc gcg cag atc acc ctg atc ggc gat tcg gcg        480
Gln Arg Gly Tyr Ala Pro Ala Gln Ile Thr Leu Ile Gly Asp Ser Ala           
145                 150                 155                 160           

ggc ggc aac ctg gta ctg gtg acc gcg cag aaa ctg gcc gcg ctc aag        528
Gly Gly Asn Leu Val Leu Val Thr Ala Gln Lys Leu Ala Ala Leu Lys           
                165                 170                 175               

ctg ccg ctg ccg gcc tcg ctg gtc tgc ttt tca ccg gtc acc gac atg        576
Leu Pro Leu Pro Ala Ser Leu Val Cys Phe Ser Pro Val Thr Asp Met           
            180                 185                 190                   

acc gcc gaa cag ctg cac gcg cct gcg gcc ggc gat cca ctg ctg cat        624
Thr Ala Glu Gln Leu His Ala Pro Ala Ala Gly Asp Pro Leu Leu His           
        195                 200                 205                       

ccg tcc tgg cta gac agc gct cgc gac gcc tac tgc ccg gcc ggg ctg        672
Pro Ser Trp Leu Asp Ser Ala Arg Asp Ala Tyr Cys Pro Ala Gly Leu           
    210                 215                 220                           

gac cgc gcc gac ccg atg gtg tcg ccg ctg ttc ggc cag ctc aag ggc        720
Asp Arg Ala Asp Pro Met Val Ser Pro Leu Phe Gly Gln Leu Lys Gly           
225                 230                 235                 240           

ctg ccg cca ttg ctg ctg cag gtc ggt gaa gac gag gtg ctg ctg aac        768
Leu Pro Pro Leu Leu Leu Gln Val Gly Glu Asp Glu Val Leu Leu Asn           
                245                 250                 255               

gac agc ctg cgg ctg gcc gaa gcc gcg cgc gca gcc gac gtt gcc gta        816
Asp Ser Leu Arg Leu Ala Glu Ala Ala Arg Ala Ala Asp Val Ala Val           
            260                 265                 270                   

cgg ctg gag cgc tac gaa gac ctg tgg cat gta ttt cag gcc cat gcc        864
Arg Leu Glu Arg Tyr Glu Asp Leu Trp His Val Phe Gln Ala His Ala           
        275                 280                 285                       

ggg ctg ctg cac agc gcc gat gcg gcg ctg cag cgg gtg gtg gac ttt        912
Gly Leu Leu His Ser Ala Asp Ala Ala Leu Gln Arg Val Val Asp Phe           
    290                 295                 300                           

gtg aac agc gct cag acc gac tcg gta agc tga                            945
Val Asn Ser Ala Gln Thr Asp Ser Val Ser                                   
305                 310                                                   


<210>  2
<211>  314
<212>  PRT
<213>  Unknown

<220>
<223>  Synthetic Construct

<400>  2

Met Ser Arg Tyr Val Asp Glu Leu Tyr Arg Asn Pro Gly Gln Pro Gly 
1               5                   10                  15      


Leu Arg Ala Leu Leu Arg Gly Met Leu Lys Leu Leu Phe Arg Gly Leu 
            20                  25                  30          


Ile Arg Pro Pro Val Pro Phe Ala Val Gln Ala Leu Val Leu Arg Leu 
        35                  40                  45              


Leu Thr Leu Gly Met Pro Leu Ala Arg Gly Val Thr Arg Ser Thr Glu 
    50                  55                  60                  


Gln Ile Ala Gly Arg Pro Cys Met Trp His Arg Pro Ala Ala Gly Gly 
65                  70                  75                  80  


Asn Gly Arg Val Leu Leu Tyr Leu His Gly Gly Ala Phe Val Ile Gly 
                85                  90                  95      


Ser Pro Gln Thr His Arg Gly Ile Cys Ser Ala Leu Ala Ser Arg Gly 
            100                 105                 110         


Gln Phe Asp Val Cys Ala Leu Asp Tyr Arg Leu Ala Pro Ala His Pro 
        115                 120                 125             


Ala Pro Ala Ala Cys Asp Asp Ala Val Ala Ala Tyr Gln Ala Leu Leu 
    130                 135                 140                 


Gln Arg Gly Tyr Ala Pro Ala Gln Ile Thr Leu Ile Gly Asp Ser Ala 
145                 150                 155                 160 


Gly Gly Asn Leu Val Leu Val Thr Ala Gln Lys Leu Ala Ala Leu Lys 
                165                 170                 175     


Leu Pro Leu Pro Ala Ser Leu Val Cys Phe Ser Pro Val Thr Asp Met 
            180                 185                 190         


Thr Ala Glu Gln Leu His Ala Pro Ala Ala Gly Asp Pro Leu Leu His 
        195                 200                 205             


Pro Ser Trp Leu Asp Ser Ala Arg Asp Ala Tyr Cys Pro Ala Gly Leu 
    210                 215                 220                 


Asp Arg Ala Asp Pro Met Val Ser Pro Leu Phe Gly Gln Leu Lys Gly 
225                 230                 235                 240 


Leu Pro Pro Leu Leu Leu Gln Val Gly Glu Asp Glu Val Leu Leu Asn 
                245                 250                 255     


Asp Ser Leu Arg Leu Ala Glu Ala Ala Arg Ala Ala Asp Val Ala Val 
            260                 265                 270         


Arg Leu Glu Arg Tyr Glu Asp Leu Trp His Val Phe Gln Ala His Ala 
        275                 280                 285             


Gly Leu Leu His Ser Ala Asp Ala Ala Leu Gln Arg Val Val Asp Phe 
    290                 295                 300                 


Val Asn Ser Ala Gln Thr Asp Ser Val Ser 
305                 310                 


<210>  3
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Primer Est F (forward primer)

<400>  3
atgtccaggt acgttgatga gc                                                22


<210>  4
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Primer  EstR (reverse primer)

<400>  4
tcagcttacc gagtcggtct                                                   20


<210>  5
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  Lipase/Esterase motif


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(12)
<223>  Xaa can be any naturally occurring amino acid

<400>  5

His Gly Gly Xaa Phe Xaa Xaa Xaa Xaa Xaa Xaa Xaa His 
1               5                   10              


<210>  6
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  Lipase/esterase motif


<220>
<221>  misc_feature
<222>  (2)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Xaa can be any naturally occurring amino acid

<400>  6

Val Xaa Xaa Xaa Xaa Tyr Xaa Xaa Xaa Pro Xaa Ala 
1               5                   10          


<210>  7
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  Lipase/esterase motif


<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  7

Gly Asp Ser Ala Gly Xaa Xaa Leu 
1               5               


<210>  8
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Motif

<400>  8

Gly Asp Ser Ala Gly 
1               5   


<210>  9
<211>  308
<212>  PRT
<213>  Pseudomonas aeruginosa


<220>
<221>  MISC_FEATURE
<223>  AF034088

<400>  9

Met Pro Leu Asp Lys Gln Ile Ala Ala Val Leu Gln Gln Phe Ser Glu 
1               5                   10                  15      


Leu Pro Ala Pro Asp Phe Ser Gln Leu Asp Ala Ala Gln Tyr Arg Gln 
            20                  25                  30          


Phe Cys Asp Asn Leu Leu Pro Ala Ile Pro Gly Asp Pro Met Ile Glu 
        35                  40                  45              


Val Arg Asn Leu Arg Val Ala Ala Ala Ala Gly Glu Leu Asp Ala Arg 
    50                  55                  60                  


Leu Tyr Arg Pro Leu Glu Glu Asp Asn Leu Pro Leu Leu Val Phe Phe 
65                  70                  75                  80  


His Gly Gly Gly Phe Val Met Gly Asn Leu Asp Thr His Asp Asn Leu 
                85                  90                  95      


Cys Arg Ser Leu Ala Ser Gln Thr Glu Ala Val Val Val Ser Val Ala 
            100                 105                 110         


Tyr Arg Leu Ala Pro Glu Asn His Phe Pro Ala Ala Pro Leu Asp Cys 
        115                 120                 125             


Tyr Ala Ala Thr Cys Trp Leu Val Glu His Ala Ala Glu Leu Gly Val 
    130                 135                 140                 


Asp Gly Arg Arg Leu Ala Leu Ala Gly Asp Ser Ala Gly Gly Asn Leu 
145                 150                 155                 160 


Ala Leu Ala Val Ser Arg Leu Ala Ala Gln Arg Gln Gly Pro Lys Ile 
                165                 170                 175     


Ser Tyr Gln Cys Leu Phe Tyr Pro Val Thr Asp Ala Arg Cys Asp Ser 
            180                 185                 190         


Gln Ser Tyr Glu Glu Phe Ala Glu Gly Tyr Phe Leu Thr Gly Ala Met 
        195                 200                 205             


Met Tyr Trp Phe Trp Gln Gln Tyr Leu Gln Asp Thr Gly Gln Gly Asp 
    210                 215                 220                 


Asp Pro Leu Ala Ser Pro Leu Arg Ala Glu Thr Leu Ala Asp Leu Pro 
225                 230                 235                 240 


Pro Thr Thr Leu Ile Thr Ala Glu Phe Asp Pro Leu Arg Asp Glu Gly 
                245                 250                 255     


Glu Ala Phe Ala Leu Arg Leu Gln Gln Ala Gly Val Ser Val Arg Val 
            260                 265                 270         


Gln Arg Cys Glu Gly Met Ile His Gly Phe Ile Ser Met Ala Pro Phe 
        275                 280                 285             


Val Glu Arg Ala Ala His Ala Leu Ser Asp Ala Ala Ala Asp Leu Arg 
    290                 295                 300                 


Arg Ala Leu Asn 
305             


<210>  10
<211>  311
<212>  PRT
<213>  Archaeoglobus fulgidus


<220>
<221>  MISC_FEATURE
<223>  AE00985

<400>  10

Met Leu Asp Met Pro Ile Asp Pro Val Tyr Tyr Gln Leu Ala Glu Tyr 
1               5                   10                  15      


Phe Asp Ser Leu Pro Lys Phe Asp Gln Phe Ser Ser Ala Arg Glu Tyr 
            20                  25                  30          


Arg Glu Ala Ile Asn Arg Ile Tyr Glu Glu Arg Asn Arg Gln Leu Ser 
        35                  40                  45              


Gln His Glu Arg Val Glu Arg Val Glu Asp Arg Thr Ile Lys Gly Arg 
    50                  55                  60                  


Asn Gly Asp Ile Arg Val Arg Val Tyr Gln Gln Lys Pro Asp Ser Pro 
65                  70                  75                  80  


Val Leu Val Tyr Tyr His Gly Gly Gly Phe Val Ile Cys Ser Ile Glu 
                85                  90                  95      


Ser His Asp Ala Leu Cys Arg Arg Ile Ala Arg Leu Ser Asn Ser Thr 
            100                 105                 110         


Val Val Ser Val Asp Tyr Arg Leu Ala Pro Glu His Lys Phe Pro Ala 
        115                 120                 125             


Ala Val Tyr Asp Cys Tyr Asp Ala Thr Lys Trp Val Ala Glu Asn Ala 
    130                 135                 140                 


Glu Glu Leu Arg Ile Asp Pro Ser Lys Ile Phe Val Gly Gly Asp Ser 
145                 150                 155                 160 


Ala Gly Gly Asn Leu Ala Ala Ala Val Ser Ile Met Ala Arg Asp Ser 
                165                 170                 175     


Gly Glu Asp Phe Ile Lys His Gln Ile Leu Ile Tyr Pro Val Val Asn 
            180                 185                 190         


Phe Val Ala Pro Thr Pro Ser Leu Leu Glu Phe Gly Glu Gly Leu Trp 
        195                 200                 205             


Ile Leu Asp Gln Lys Ile Met Ser Trp Phe Ser Glu Gln Tyr Phe Ser 
    210                 215                 220                 


Arg Glu Glu Asp Lys Phe Asn Pro Leu Ala Ser Val Ile Phe Ala Asp 
225                 230                 235                 240 


Leu Glu Asn Leu Pro Pro Ala Leu Ile Ile Thr Ala Glu Tyr Asp Pro 
                245                 250                 255     


Leu Arg Asp Glu Gly Glu Val Phe Gly Gln Met Leu Arg Arg Ala Gly 
            260                 265                 270         


Val Glu Ala Ser Ile Val Arg Tyr Arg Gly Val Leu His Gly Phe Ile 
        275                 280                 285             


Asn Tyr Tyr Pro Val Leu Lys Ala Ala Arg Asp Ala Ile Asn Gln Ile 
    290                 295                 300                 


Ala Ala Leu Leu Val Phe Asp 
305                 310     


<210>  11
<211>  327
<212>  PRT
<213>  Alcaligenes eutrophus


<220>
<221>  MISC_FEATURE
<223>  L36817

<400>  11

Met Gln Arg Arg His Phe Ile Ala Arg Ala Gly Ile Ala Ala Ala Thr 
1               5                   10                  15      


Ala Ala Leu Gly Leu Ala Ala Met Pro Ala Gln Ala Gln Ala Asp Lys 
            20                  25                  30          


Phe Pro Gln Arg Pro Ile Arg Leu Val Ile Gly Tyr Thr Ala Gly Gly 
        35                  40                  45              


Ser Thr Asp Ile Pro Phe Arg Val Leu Ala Asp Asn Ala Ser Lys Ile 
    50                  55                  60                  


Leu Gly Gln Pro Val Ile Val Glu Asn Lys Pro Gly Ala Gly Gly Val 
65                  70                  75                  80  


Leu Pro Ala Gln Met Met Gln Ser Thr Ala Pro Asp Gly Tyr Thr Leu 
                85                  90                  95      


Ala Gln Val Ala Met Pro Val Tyr Arg Leu Pro Tyr Thr Thr Lys Ile 
            100                 105                 110         


Asn Trp Asp Pro Val Lys Asp Leu Asn Tyr Ile Ile Asn Leu Ala Gly 
        115                 120                 125             


Tyr Ser Phe Gly Leu Val Val Pro Ala Asp Ser Pro Ile Lys Thr Met 
    130                 135                 140                 


Gln Glu Tyr Ile Ala Tyr Ala Lys Ala Asn Pro Gly Lys Leu Thr Tyr 
145                 150                 155                 160 


Gly Ser Pro Gly Ser Met Thr Thr Leu His Leu Thr Met Glu Glu Leu 
                165                 170                 175     


Ala Met Lys Gln Gly Val Gln Phe Ser His Ile Pro Tyr Lys Gly Asn 
            180                 185                 190         


Ser Glu Ser Met Gln Ala Leu Leu Gly Gly His Val Met Ser Val Ala 
        195                 200                 205             


Asp Thr Pro Ala Trp Ala Pro Tyr Val Glu Gln Gly Lys Leu Arg Leu 
    210                 215                 220                 


Leu Ser Thr Trp Gly Glu Lys Arg Ser Ala Arg Phe Pro Ser Val Pro 
225                 230                 235                 240 


Thr Leu Lys Glu Leu Gly Ile Gly Ile Val Gln Thr Ser Pro Phe Gly 
                245                 250                 255     


Leu Val Ala Pro Lys Gly Thr Asp Pro Lys Ile Val Gln Lys Leu His 
            260                 265                 270         


Asp Ala Phe Lys Lys Ala Met Asp Met Pro Asn Tyr Arg Glu Ser Leu 
        275                 280                 285             


Ala Lys Phe Asp Met Glu Pro Tyr Tyr Met Asn Ser Gln Gln Tyr Ala 
    290                 295                 300                 


Gln Phe Ala Ala Glu Thr Val Lys Lys Glu Lys Ala Ile Ile Glu Lys 
305                 310                 315                 320 


Leu Gly Leu Ala Lys Ala Gln 
                325         


<210>  12
<211>  433
<212>  PRT
<213>  Moraxella sp.


<220>
<221>  MISC_FEATURE
<223>  X53868

<400>  12

Met Pro Ile Leu Pro Val Pro Ala Leu Asn Ala Leu Leu Thr Lys Thr 
1               5                   10                  15      


Ile Lys Thr Ile Lys Thr Gly Ala Ala Lys Asn Ala His Gln His His 
            20                  25                  30          


Val Leu His His Thr Leu Lys Gly Leu Asp Asn Leu Pro Ala Pro Val 
        35                  40                  45              


Leu Glu Arg Ile Asn Arg Arg Leu Lys Ala Ser Thr Ala Glu Gln Tyr 
    50                  55                  60                  


Pro Leu Ala Asp Ala His Leu Arg Leu Ile Leu Ala Ile Ser Asn Lys 
65                  70                  75                  80  


Leu Lys Arg Pro Leu Ala Ile Asp Lys Leu Pro Lys Leu Arg Gln Lys 
                85                  90                  95      


Phe Gly Thr Asp Ala Val Ser Leu Gln Ala Pro Ser Val Trp Gln Gln 
            100                 105                 110         


Asn Ala Asp Ala Ser Gly Ser Thr Glu Asn Ala Val Ser Trp Gln Asp 
        115                 120                 125             


Lys Thr Ile Ala Asn Ala Asp Gly Gly Asp Met Thr Val Arg Cys Tyr 
    130                 135                 140                 


Gln Lys Ser Thr Gln Asn Ser Glu Arg Lys Ser Thr Asp Glu Ala Ala 
145                 150                 155                 160 


Met Leu Phe Phe His Gly Gly Gly Phe Cys Ile Gly Asp Ile Asp Thr 
                165                 170                 175     


His His Glu Phe Cys His Thr Val Cys Ala Gln Thr Gly Trp Ala Val 
            180                 185                 190         


Val Ser Val Asp Tyr Arg Met Ala Pro Glu Tyr Pro Ala Pro Thr Ala 
        195                 200                 205             


Leu Lys Asp Cys Leu Ala Ala Tyr Ala Trp Leu Ala Glu His Ser Gln 
    210                 215                 220                 


Ser Leu Gly Ala Ser Pro Ser Arg Ile Val Leu Ser Gly Asp Ser Ala 
225                 230                 235                 240 


Gly Gly Cys Leu Ala Ala Leu Val Ala Gln Gln Val Ile Lys Pro Ile 
                245                 250                 255     


Asp Ala Leu Trp Gln Asp Asn Asn Gln Ala Pro Ala Ala Asp Lys Lys 
            260                 265                 270         


Val Asn Asp Thr Phe Lys Asn Ser Leu Ala Asp Leu Pro Arg Pro Leu 
        275                 280                 285             


Ala Gln Leu Pro Leu Tyr Pro Val Thr Asp Tyr Glu Ala Glu Tyr Pro 
    290                 295                 300                 


Ser Trp Glu Leu Tyr Gly Glu Gly Leu Leu Leu Asp His Asn Asp Ala 
305                 310                 315                 320 


Glu Val Phe Asn Ser Ala Tyr Thr Gln His Ser Gly Leu Pro Gln Ser 
                325                 330                 335     


His Pro Leu Ile Ser Val Met His Gly Asp Asn Thr Gln Leu Cys Pro 
            340                 345                 350         


Ser Tyr Ile Val Val Ala Glu Leu Asp Ile Leu Arg Asp Glu Gly Leu 
        355                 360                 365             


Ala Tyr Ala Glu Leu Leu Gln Lys Glu Gly Val Gln Val Gln Thr Tyr 
    370                 375                 380                 


Thr Val Leu Gly Ala Pro His Gly Phe Ile Asn Leu Met Ser Val His 
385                 390                 395                 400 


Gln Gly Leu Gly Asn Gln Thr Thr Tyr Ile Ile Asn Glu Phe Ala Cys 
                405                 410                 415     


Leu Val Gln Asn Leu Leu Thr Ser Glu Gly Asp Lys Pro Asn Leu Arg 
            420                 425                 430         


Ala 
    


<210>  13
<211>  319
<212>  PRT
<213>  Escherichia coli


<220>
<221>  MISC_FEATURE
<223>  AE00153

<400>  13

Met Lys Pro Glu Asn Lys Leu Pro Val Leu Asp Leu Ile Ser Ala Glu 
1               5                   10                  15      


Met Lys Thr Val Val Asn Thr Leu Gln Pro Asp Leu Pro Pro Trp Pro 
            20                  25                  30          


Ala Thr Gly Thr Ile Ala Glu Gln Arg Gln Tyr Tyr Thr Leu Glu Arg 
        35                  40                  45              


Arg Phe Trp Asn Ala Gly Ala Pro Glu Met Ala Thr Arg Ala Tyr Met 
    50                  55                  60                  


Val Pro Thr Lys Tyr Gly Gln Val Glu Thr Arg Leu Phe Cys Pro Gln 
65                  70                  75                  80  


Pro Asp Ser Pro Ala Thr Leu Phe Tyr Leu His Gly Gly Gly Phe Ile 
                85                  90                  95      


Leu Gly Asn Leu Asp Thr His Asp Arg Ile Met Arg Leu Leu Ala Ser 
            100                 105                 110         


Tyr Ser Gln Cys Thr Val Ile Gly Ile Asp Tyr Thr Leu Ser Pro Glu 
        115                 120                 125             


Ala Arg Phe Pro Gln Ala Ile Glu Glu Ile Val Ala Ala Cys Cys Tyr 
    130                 135                 140                 


Phe His Gln Gln Ala Glu Asp Tyr Gln Ile Asn Met Ser Arg Ile Gly 
145                 150                 155                 160 


Phe Ala Gly Asp Ser Ala Gly Ala Met Leu Ala Leu Ala Ser Ala Leu 
                165                 170                 175     


Trp Leu Arg Asp Lys Gln Ile Asp Cys Gly Lys Val Ala Gly Val Leu 
            180                 185                 190         


Leu Trp Tyr Gly Leu Tyr Gly Leu Arg Asp Ser Val Thr Arg Arg Leu 
        195                 200                 205             


Leu Gly Gly Val Trp Asp Gly Leu Thr Gln Gln Asp Leu Gln Met Tyr 
    210                 215                 220                 


Glu Glu Ala Tyr Leu Ser Asn Asp Ala Asp Arg Glu Ser Pro Tyr Tyr 
225                 230                 235                 240 


Cys Leu Phe Asn Asn Asp Leu Thr Arg Glu Val Pro Pro Cys Phe Ile 
                245                 250                 255     


Ala Gly Ala Glu Phe Asp Pro Leu Leu Asp Asp Ser Arg Leu Leu Tyr 
            260                 265                 270         


Gln Thr Leu Ala Ala His Gln Gln Pro Cys Glu Phe Lys Leu Tyr Pro 
        275                 280                 285             


Gly Thr Leu His Ala Phe Leu His Tyr Ser Arg Met Met Lys Thr Ala 
    290                 295                 300                 


Asp Glu Ala Leu Arg Asp Gly Ala Gln Phe Phe Thr Ala Gln Leu 
305                 310                 315                 



