                         SEQUENCE LISTING

<110>  Wacker Chemie AG
 
<120>  Mikroorganismenstamm und Verfahren zur fermentativen Herstellung 
       von C4-Verbindungen aus C5-Zuckern

<130>  poxB

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  1719
<212>  DNA
<213>  Raoultella terrigena


<220>
<221>  CDS
<222>  (1)..(1719)

<400>  1
atg aaa cag acc gtg gcc gca tac gta gcg aaa act ctt gaa cag gcc         48
Met Lys Gln Thr Val Ala Ala Tyr Val Ala Lys Thr Leu Glu Gln Ala           
1               5                   10                  15                

ggc gtg aag cgc atc tgg ggc gtc acc ggc gat tca ctc aac gga ttg         96
Gly Val Lys Arg Ile Trp Gly Val Thr Gly Asp Ser Leu Asn Gly Leu           
            20                  25                  30                    

agc gac agc ctg aac cgc atg ggc acc att gca tgg atg ccg acg cgc        144
Ser Asp Ser Leu Asn Arg Met Gly Thr Ile Ala Trp Met Pro Thr Arg           
        35                  40                  45                        

cat gaa gaa gtt gcc gcg ttt gcc gcc ggc gcc gaa gcc cag ctt acc        192
His Glu Glu Val Ala Ala Phe Ala Ala Gly Ala Glu Ala Gln Leu Thr           
    50                  55                  60                            

ggc gag ctg gcg gtc tgc gcg ggc tcc tgc ggc ccg ggc aat ctt cat        240
Gly Glu Leu Ala Val Cys Ala Gly Ser Cys Gly Pro Gly Asn Leu His           
65                  70                  75                  80            

ctg atc aac ggc ctt ttt gac tgt cac cgc aac cac gtt ccg gtg ctg        288
Leu Ile Asn Gly Leu Phe Asp Cys His Arg Asn His Val Pro Val Leu           
                85                  90                  95                

gcc atc gcc gcc cac att ccc tcc agc gaa atc ggc agc ggc tat ttt        336
Ala Ile Ala Ala His Ile Pro Ser Ser Glu Ile Gly Ser Gly Tyr Phe           
            100                 105                 110                   

cag gaa acc cat ccg cag gag ctg ttc cgc gaa tgc agc cac tat tgt        384
Gln Glu Thr His Pro Gln Glu Leu Phe Arg Glu Cys Ser His Tyr Cys           
        115                 120                 125                       

gag ctg gtc tcc acc ccg gaa cag atc ccg cag gtg ctg gcc atc gcc        432
Glu Leu Val Ser Thr Pro Glu Gln Ile Pro Gln Val Leu Ala Ile Ala           
    130                 135                 140                           

atg cgc aaa gcg gtc ctc aac cgc ggc gtt tcg gtg gtg gtg ctg ccg        480
Met Arg Lys Ala Val Leu Asn Arg Gly Val Ser Val Val Val Leu Pro           
145                 150                 155                 160           

gga gac gtg gcg ctg aag gcc gcc ccg gaa ggc gcc agc agc cac tgg        528
Gly Asp Val Ala Leu Lys Ala Ala Pro Glu Gly Ala Ser Ser His Trp           
                165                 170                 175               

tac tcc gcc ccg ctg ccg gag gtg acg ccg ccg gcg gaa gaa ata gct        576
Tyr Ser Ala Pro Leu Pro Glu Val Thr Pro Pro Ala Glu Glu Ile Ala           
            180                 185                 190                   

cgt ctg gcg cag ctg ctg cgc tac tcc agc aac att gcc ctg atg tgc        624
Arg Leu Ala Gln Leu Leu Arg Tyr Ser Ser Asn Ile Ala Leu Met Cys           
        195                 200                 205                       

ggc agc ggc tgt gcc gga gcc cat gaa gag ctg gtc gca ttc gcc gcc        672
Gly Ser Gly Cys Ala Gly Ala His Glu Glu Leu Val Ala Phe Ala Ala           
    210                 215                 220                           

aag ctc aag gcg ccc atc gtc cat gcc ctg cgc ggc aag gag cac gtt        720
Lys Leu Lys Ala Pro Ile Val His Ala Leu Arg Gly Lys Glu His Val           
225                 230                 235                 240           

gag tac gac aac cct tat gat gtc gga atg acc ggg ctg atc ggc ttc        768
Glu Tyr Asp Asn Pro Tyr Asp Val Gly Met Thr Gly Leu Ile Gly Phe           
                245                 250                 255               

tcg tcc ggc ttc cac acc atg atg aac gcc gac acc ctg gtg ctt ctc        816
Ser Ser Gly Phe His Thr Met Met Asn Ala Asp Thr Leu Val Leu Leu           
            260                 265                 270                   

ggc acc cag ttc ccc tac cgg ccc ttc tat ccc agc agc gcg aaa atc        864
Gly Thr Gln Phe Pro Tyr Arg Pro Phe Tyr Pro Ser Ser Ala Lys Ile           
        275                 280                 285                       

att cag att gat atc aat ccc ggc agc atc ggc gcg cac agc aaa gtc        912
Ile Gln Ile Asp Ile Asn Pro Gly Ser Ile Gly Ala His Ser Lys Val           
    290                 295                 300                           

gat atg gcg ctg atc ggc gat atc aaa tcg acc cta aaa gcg ctg ctg        960
Asp Met Ala Leu Ile Gly Asp Ile Lys Ser Thr Leu Lys Ala Leu Leu           
305                 310                 315                 320           

ccg cat ctg gaa gag aag acg gat cgc cgc ttc ctc gac aaa gcc ctt       1008
Pro His Leu Glu Glu Lys Thr Asp Arg Arg Phe Leu Asp Lys Ala Leu           
                325                 330                 335               

gag cac tac cgc gag gcg cgt aag ggg ctc gac gat ctg gcc aag ccg       1056
Glu His Tyr Arg Glu Ala Arg Lys Gly Leu Asp Asp Leu Ala Lys Pro           
            340                 345                 350                   

agc gat aag gcc att cac ccc cag tac ctg gcg cag cag ata agc cgc       1104
Ser Asp Lys Ala Ile His Pro Gln Tyr Leu Ala Gln Gln Ile Ser Arg           
        355                 360                 365                       

ttc gcc gct gac gat gcc att ttc acc tgc gac gtc ggt acg cca acc       1152
Phe Ala Ala Asp Asp Ala Ile Phe Thr Cys Asp Val Gly Thr Pro Thr           
    370                 375                 380                           

gtc tgg gcc gca cgc tat ctg aag atg aac ggc agg cgc cgg ctg atc       1200
Val Trp Ala Ala Arg Tyr Leu Lys Met Asn Gly Arg Arg Arg Leu Ile           
385                 390                 395                 400           

ggc tcg ttt aac cac ggc tca atg gcc aac gcc atg ccg cag gcg att       1248
Gly Ser Phe Asn His Gly Ser Met Ala Asn Ala Met Pro Gln Ala Ile           
                405                 410                 415               

ggc gcg aag gca acg gcg ccg gag cgt cag gtg gtg gcg atg tgc ggc       1296
Gly Ala Lys Ala Thr Ala Pro Glu Arg Gln Val Val Ala Met Cys Gly           
            420                 425                 430                   

gac ggc ggc ttc agc atg ctg atg ggc gac ttc ctc tcg ctg gcg cag       1344
Asp Gly Gly Phe Ser Met Leu Met Gly Asp Phe Leu Ser Leu Ala Gln           
        435                 440                 445                       

atg aag ctg ccg gta aaa atc gtg atc ttt aac aac agc gtg ctg gga       1392
Met Lys Leu Pro Val Lys Ile Val Ile Phe Asn Asn Ser Val Leu Gly           
    450                 455                 460                           

ttt gtc gca atg gag atg aag gcc ggg ggc tat ctg acc gac ggt acc       1440
Phe Val Ala Met Glu Met Lys Ala Gly Gly Tyr Leu Thr Asp Gly Thr           
465                 470                 475                 480           

gag ctg cac gac acc aat ttc gcc cgc att gct gaa gcc tgc ggc ata       1488
Glu Leu His Asp Thr Asn Phe Ala Arg Ile Ala Glu Ala Cys Gly Ile           
                485                 490                 495               

aaa ggc att cgc gtc gaa aaa gcc tct gag gtc gac gac gcg ctg caa       1536
Lys Gly Ile Arg Val Glu Lys Ala Ser Glu Val Asp Asp Ala Leu Gln           
            500                 505                 510                   

acc gcg ttt agc acc gac ggc ccg gtg ctg gtc gat gtg gtg gtt gcc       1584
Thr Ala Phe Ser Thr Asp Gly Pro Val Leu Val Asp Val Val Val Ala           
        515                 520                 525                       

aag gaa gag ctg gcg atc ccg ccg caa atc aag ctg gat cag gcc aag       1632
Lys Glu Glu Leu Ala Ile Pro Pro Gln Ile Lys Leu Asp Gln Ala Lys           
    530                 535                 540                           

ggt ttc agc ctg tat atg ctg cgc gcc atc atc agc gga cgc gga gat       1680
Gly Phe Ser Leu Tyr Met Leu Arg Ala Ile Ile Ser Gly Arg Gly Asp           
545                 550                 555                 560           

gaa gtt atc gaa ctg gcg aaa acc aac tgg ctc agg taa                   1719
Glu Val Ile Glu Leu Ala Lys Thr Asn Trp Leu Arg                           
                565                 570                                   


<210>  2
<211>  572
<212>  PRT
<213>  Raoultella terrigena

<400>  2

Met Lys Gln Thr Val Ala Ala Tyr Val Ala Lys Thr Leu Glu Gln Ala 
1               5                   10                  15      


Gly Val Lys Arg Ile Trp Gly Val Thr Gly Asp Ser Leu Asn Gly Leu 
            20                  25                  30          


Ser Asp Ser Leu Asn Arg Met Gly Thr Ile Ala Trp Met Pro Thr Arg 
        35                  40                  45              


His Glu Glu Val Ala Ala Phe Ala Ala Gly Ala Glu Ala Gln Leu Thr 
    50                  55                  60                  


Gly Glu Leu Ala Val Cys Ala Gly Ser Cys Gly Pro Gly Asn Leu His 
65                  70                  75                  80  


Leu Ile Asn Gly Leu Phe Asp Cys His Arg Asn His Val Pro Val Leu 
                85                  90                  95      


Ala Ile Ala Ala His Ile Pro Ser Ser Glu Ile Gly Ser Gly Tyr Phe 
            100                 105                 110         


Gln Glu Thr His Pro Gln Glu Leu Phe Arg Glu Cys Ser His Tyr Cys 
        115                 120                 125             


Glu Leu Val Ser Thr Pro Glu Gln Ile Pro Gln Val Leu Ala Ile Ala 
    130                 135                 140                 


Met Arg Lys Ala Val Leu Asn Arg Gly Val Ser Val Val Val Leu Pro 
145                 150                 155                 160 


Gly Asp Val Ala Leu Lys Ala Ala Pro Glu Gly Ala Ser Ser His Trp 
                165                 170                 175     


Tyr Ser Ala Pro Leu Pro Glu Val Thr Pro Pro Ala Glu Glu Ile Ala 
            180                 185                 190         


Arg Leu Ala Gln Leu Leu Arg Tyr Ser Ser Asn Ile Ala Leu Met Cys 
        195                 200                 205             


Gly Ser Gly Cys Ala Gly Ala His Glu Glu Leu Val Ala Phe Ala Ala 
    210                 215                 220                 


Lys Leu Lys Ala Pro Ile Val His Ala Leu Arg Gly Lys Glu His Val 
225                 230                 235                 240 


Glu Tyr Asp Asn Pro Tyr Asp Val Gly Met Thr Gly Leu Ile Gly Phe 
                245                 250                 255     


Ser Ser Gly Phe His Thr Met Met Asn Ala Asp Thr Leu Val Leu Leu 
            260                 265                 270         


Gly Thr Gln Phe Pro Tyr Arg Pro Phe Tyr Pro Ser Ser Ala Lys Ile 
        275                 280                 285             


Ile Gln Ile Asp Ile Asn Pro Gly Ser Ile Gly Ala His Ser Lys Val 
    290                 295                 300                 


Asp Met Ala Leu Ile Gly Asp Ile Lys Ser Thr Leu Lys Ala Leu Leu 
305                 310                 315                 320 


Pro His Leu Glu Glu Lys Thr Asp Arg Arg Phe Leu Asp Lys Ala Leu 
                325                 330                 335     


Glu His Tyr Arg Glu Ala Arg Lys Gly Leu Asp Asp Leu Ala Lys Pro 
            340                 345                 350         


Ser Asp Lys Ala Ile His Pro Gln Tyr Leu Ala Gln Gln Ile Ser Arg 
        355                 360                 365             


Phe Ala Ala Asp Asp Ala Ile Phe Thr Cys Asp Val Gly Thr Pro Thr 
    370                 375                 380                 


Val Trp Ala Ala Arg Tyr Leu Lys Met Asn Gly Arg Arg Arg Leu Ile 
385                 390                 395                 400 


Gly Ser Phe Asn His Gly Ser Met Ala Asn Ala Met Pro Gln Ala Ile 
                405                 410                 415     


Gly Ala Lys Ala Thr Ala Pro Glu Arg Gln Val Val Ala Met Cys Gly 
            420                 425                 430         


Asp Gly Gly Phe Ser Met Leu Met Gly Asp Phe Leu Ser Leu Ala Gln 
        435                 440                 445             


Met Lys Leu Pro Val Lys Ile Val Ile Phe Asn Asn Ser Val Leu Gly 
    450                 455                 460                 


Phe Val Ala Met Glu Met Lys Ala Gly Gly Tyr Leu Thr Asp Gly Thr 
465                 470                 475                 480 


Glu Leu His Asp Thr Asn Phe Ala Arg Ile Ala Glu Ala Cys Gly Ile 
                485                 490                 495     


Lys Gly Ile Arg Val Glu Lys Ala Ser Glu Val Asp Asp Ala Leu Gln 
            500                 505                 510         


Thr Ala Phe Ser Thr Asp Gly Pro Val Leu Val Asp Val Val Val Ala 
        515                 520                 525             


Lys Glu Glu Leu Ala Ile Pro Pro Gln Ile Lys Leu Asp Gln Ala Lys 
    530                 535                 540                 


Gly Phe Ser Leu Tyr Met Leu Arg Ala Ile Ile Ser Gly Arg Gly Asp 
545                 550                 555                 560 


Glu Val Ile Glu Leu Ala Lys Thr Asn Trp Leu Arg 
                565                 570         


<210>  3
<211>  70
<212>  DNA
<213>  artificial sequence

<220>
<223>  PCR primer

<400>  3
ccgcatgggc accattgcat ggatgccgac gcgccatgaa gaagttgccg gtgtaggctg       60

gagctgcttc                                                              70


<210>  4
<211>  70
<212>  DNA
<213>  artificial sequence

<220>
<223>  PCR primer

<400>  4
tcgccagctc ttccttggca accaccacat cgaccagcac cgggccgtcg ccgtcgacct       60

gcagttcgaa                                                              70


<210>  5
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  PCR primer

<400>  5
atgaaacaga ccgtggccgc atacg                                             25


<210>  6
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  PCR primer

<400>  6
ttacctgagc cagttggttt tcgcc                                             25


<210>  7
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  PCR primer

<400>  7
ccgacctgtc cggtgccctg aatg                                              24


