                         SEQUENCE LISTING

<110>  Kaufman, Michael D.
       Flynn, Daniel L.
       Ahn, YuMi
       Vogeti, Lakshminarayana
       Caldwell, Timothy Malcolm
 
<120>  2-AMINOPYRIMIDIN-6-ONES AND ANALOGS EXHIBITING ANTI-CANCER AND 
       ANTI-PROLIFERATIVE ACTIVITIES

<130>  DECP-062/01US 313114-2333

<150>  US 61/792,812
<151>  2013-03-15

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  435
<212>  PRT
<213>  Homo sapiens

<400>  1

Tyr Lys Tyr Lys Gln Lys Pro Lys Tyr Gln Val Arg Trp Lys Ile Ile 
1               5                   10                  15      


Glu Ser Tyr Glu Gly Asn Ser Tyr Thr Phe Ile Asp Pro Thr Gln Leu 
            20                  25                  30          


Pro Tyr Asn Glu Lys Trp Glu Phe Pro Arg Asn Asn Leu Gln Phe Gly 
        35                  40                  45              


Lys Thr Leu Gly Ala Gly Ala Phe Gly Lys Val Val Glu Ala Thr Ala 
    50                  55                  60                  


Phe Gly Leu Gly Lys Glu Asp Ala Val Leu Lys Val Ala Val Lys Met 
65                  70                  75                  80  


Leu Lys Ser Thr Ala His Ala Asp Glu Lys Glu Ala Leu Met Ser Glu 
                85                  90                  95      


Leu Lys Ile Met Ser His Leu Gly Gln His Glu Asn Ile Val Asn Leu 
            100                 105                 110         


Leu Gly Ala Cys Thr His Gly Gly Pro Val Leu Val Ile Thr Glu Tyr 
        115                 120                 125             


Cys Cys Tyr Gly Asp Leu Leu Asn Phe Leu Arg Arg Lys Ala Glu Ala 
    130                 135                 140                 


Met Leu Gly Pro Ser Leu Ser Pro Gly Gln Asp Pro Glu Gly Gly Val 
145                 150                 155                 160 


Asp Tyr Lys Asn Ile His Leu Glu Lys Lys Tyr Val Arg Arg Asp Ser 
                165                 170                 175     


Gly Phe Ser Ser Gln Gly Val Asp Thr Tyr Val Glu Met Arg Pro Val 
            180                 185                 190         


Ser Thr Ser Ser Asn Asp Ser Phe Ser Glu Gln Asp Leu Asp Lys Glu 
        195                 200                 205             


Asp Gly Arg Pro Leu Glu Leu Arg Asp Leu Leu His Phe Ser Ser Gln 
    210                 215                 220                 


Val Ala Gln Gly Met Ala Phe Leu Ala Ser Lys Asn Cys Ile His Arg 
225                 230                 235                 240 


Asp Val Ala Ala Arg Asn Val Leu Leu Thr Asn Gly His Val Ala Lys 
                245                 250                 255     


Ile Gly Asp Phe Gly Leu Ala Arg Asp Ile Met Asn Asp Ser Asn Tyr 
            260                 265                 270         


Ile Val Lys Gly Asn Ala Arg Leu Pro Val Lys Trp Met Ala Pro Glu 
        275                 280                 285             


Ser Ile Phe Asp Cys Val Tyr Thr Val Gln Ser Asp Val Trp Ser Tyr 
    290                 295                 300                 


Gly Ile Leu Leu Trp Glu Ile Phe Ser Leu Gly Leu Asn Pro Tyr Pro 
305                 310                 315                 320 


Gly Ile Leu Val Asn Ser Lys Phe Tyr Lys Leu Val Lys Asp Gly Tyr 
                325                 330                 335     


Gln Met Ala Gln Pro Ala Phe Ala Pro Lys Asn Ile Tyr Ser Ile Met 
            340                 345                 350         


Gln Ala Cys Trp Ala Leu Glu Pro Thr His Arg Pro Thr Phe Gln Gln 
        355                 360                 365             


Ile Cys Ser Phe Leu Gln Glu Gln Ala Gln Glu Asp Arg Arg Glu Arg 
    370                 375                 380                 


Asp Tyr Thr Asn Leu Pro Ser Ser Ser Arg Ser Gly Gly Ser Gly Ser 
385                 390                 395                 400 


Ser Ser Ser Glu Leu Glu Glu Glu Ser Ser Ser Glu His Leu Thr Cys 
                405                 410                 415     


Cys Glu Gln Gly Asp Ile Ala Gln Pro Leu Leu Gln Pro Asn Asn Tyr 
            420                 425                 430         


Gln Phe Cys 
        435 


<210>  2
<211>  676
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  uKit with N-terminal GST fusion

<400>  2

Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro Thr Arg Leu Leu 
1               5                   10                  15      


Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu Tyr Glu Arg Asp 
            20                  25                  30          


Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu Gly Leu Glu Phe 
        35                  40                  45              


Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys Leu Thr Gln Ser 
    50                  55                  60                  


Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn Met Leu Gly Gly 
65                  70                  75                  80  


Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu Gly Ala Val Asp 
                85                  90                  95      


Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser Lys Asp Phe Glu Thr 
            100                 105                 110         


Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu Met Leu Lys Met Phe 
        115                 120                 125             


Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn Gly Asp His Val Thr 
    130                 135                 140                 


His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp Val Val Leu Tyr Met 
145                 150                 155                 160 


Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu Val Cys Phe Lys Lys 
                165                 170                 175     


Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr Leu Lys Ser Ser Lys 
            180                 185                 190         


Tyr Ile Trp Pro Leu Gln Gly Trp Gln Ala Thr Phe Gly Gly Gly Asp 
        195                 200                 205             


His Pro Pro Lys Ser Asp Leu Val Pro Arg His Asn Gln Thr Ser Leu 
    210                 215                 220                 


Tyr Lys Lys Ala Gly Ser Ala Ala Ala Val Leu Glu Glu Asn Leu Tyr 
225                 230                 235                 240 


Phe Gln Gly Thr Tyr Lys Tyr Leu Gln Lys Pro Met Tyr Glu Val Gln 
                245                 250                 255     


Trp Lys Val Val Glu Glu Ile Asn Gly Asn Asn Tyr Val Tyr Ile Asp 
            260                 265                 270         


Pro Thr Gln Leu Pro Tyr Asp His Lys Trp Glu Phe Pro Arg Asn Arg 
        275                 280                 285             


Leu Ser Phe Gly Lys Thr Leu Gly Ala Gly Ala Phe Gly Lys Val Val 
    290                 295                 300                 


Glu Ala Thr Ala Tyr Gly Leu Ile Lys Ser Asp Ala Ala Met Thr Val 
305                 310                 315                 320 


Ala Val Lys Met Leu Lys Pro Ser Ala His Leu Thr Glu Arg Glu Ala 
                325                 330                 335     


Leu Met Ser Glu Leu Lys Val Leu Ser Tyr Leu Gly Asn His Met Asn 
            340                 345                 350         


Ile Val Asn Leu Leu Gly Ala Cys Thr Ile Gly Gly Pro Thr Leu Val 
        355                 360                 365             


Ile Thr Glu Tyr Cys Cys Tyr Gly Asp Leu Leu Asn Phe Leu Arg Arg 
    370                 375                 380                 


Lys Arg Asp Ser Phe Ile Cys Ser Lys Gln Glu Asp His Ala Glu Ala 
385                 390                 395                 400 


Ala Leu Tyr Lys Asn Leu Leu His Ser Lys Glu Ser Ser Cys Ser Asp 
                405                 410                 415     


Ser Thr Asn Glu Tyr Met Asp Met Lys Pro Gly Val Ser Tyr Val Val 
            420                 425                 430         


Pro Thr Lys Ala Asp Lys Arg Arg Ser Val Arg Ile Gly Ser Tyr Ile 
        435                 440                 445             


Glu Arg Asp Val Thr Pro Ala Ile Met Glu Asp Asp Glu Leu Ala Leu 
    450                 455                 460                 


Asp Leu Glu Asp Leu Leu Ser Phe Ser Tyr Gln Val Ala Lys Gly Met 
465                 470                 475                 480 


Ala Phe Leu Ala Ser Lys Asn Cys Ile His Arg Asp Leu Ala Ala Arg 
                485                 490                 495     


Asn Ile Leu Leu Thr His Gly Arg Ile Thr Lys Ile Cys Asp Phe Gly 
            500                 505                 510         


Leu Ala Arg Asp Ile Lys Asn Asp Ser Asn Tyr Val Val Lys Gly Asn 
        515                 520                 525             


Ala Arg Leu Pro Val Lys Trp Met Ala Pro Glu Ser Ile Phe Asn Cys 
    530                 535                 540                 


Val Tyr Thr Phe Glu Ser Asp Val Trp Ser Tyr Gly Ile Phe Leu Trp 
545                 550                 555                 560 


Glu Leu Phe Ser Leu Gly Ser Ser Pro Tyr Pro Gly Met Pro Val Asp 
                565                 570                 575     


Ser Lys Phe Tyr Lys Met Ile Lys Glu Gly Phe Arg Met Leu Ser Pro 
            580                 585                 590         


Glu His Ala Pro Ala Glu Met Tyr Asp Ile Met Lys Thr Cys Trp Asp 
        595                 600                 605             


Ala Asp Pro Leu Lys Arg Pro Thr Phe Lys Gln Ile Val Gln Leu Ile 
    610                 615                 620                 


Glu Lys Gln Ile Ser Glu Ser Thr Asn His Ile Tyr Ser Asn Leu Ala 
625                 630                 635                 640 


Asn Cys Ser Pro Asn Arg Gln Lys Pro Val Val Asp His Ser Val Arg 
                645                 650                 655     


Ile Asn Ser Val Gly Ser Thr Ala Ser Ser Ser Gln Pro Leu Leu Val 
            660                 665                 670         


His Asp Asp Val 
        675     


<210>  3
<211>  550
<212>  PRT
<213>  Homo sapiens

<400>  3

Gln Lys Lys Pro Arg Tyr Glu Ile Arg Trp Lys Val Ile Glu Ser Val 
1               5                   10                  15      


Ser Ser Asp Gly His Glu Tyr Ile Tyr Val Asp Pro Met Gln Leu Pro 
            20                  25                  30          


Tyr Asp Ser Thr Trp Glu Leu Pro Arg Asp Gln Leu Val Leu Gly Arg 
        35                  40                  45              


Thr Leu Gly Ser Gly Ala Phe Gly Gln Val Val Glu Ala Thr Ala His 
    50                  55                  60                  


Gly Leu Ser His Ser Gln Ala Thr Met Lys Val Ala Val Lys Met Leu 
65                  70                  75                  80  


Lys Ser Thr Ala Arg Ser Ser Glu Lys Gln Ala Leu Met Ser Glu Leu 
                85                  90                  95      


Lys Ile Met Ser His Leu Gly Pro His Leu Asn Val Val Asn Leu Leu 
            100                 105                 110         


Gly Ala Cys Thr Lys Gly Gly Pro Ile Tyr Ile Ile Thr Glu Tyr Cys 
        115                 120                 125             


Arg Tyr Gly Asp Leu Val Asp Tyr Leu His Arg Asn Lys His Thr Phe 
    130                 135                 140                 


Leu Gln His His Ser Asp Lys Arg Arg Pro Pro Ser Ala Glu Leu Tyr 
145                 150                 155                 160 


Ser Asn Ala Leu Pro Val Gly Leu Pro Leu Pro Ser His Val Ser Leu 
                165                 170                 175     


Thr Gly Glu Ser Asp Gly Gly Tyr Met Asp Met Ser Lys Asp Glu Ser 
            180                 185                 190         


Val Asp Tyr Val Pro Met Leu Asp Met Lys Gly Asp Val Lys Tyr Ala 
        195                 200                 205             


Asp Ile Glu Ser Ser Asn Tyr Met Ala Pro Tyr Asp Asn Tyr Val Pro 
    210                 215                 220                 


Ser Ala Pro Glu Arg Thr Cys Arg Ala Thr Leu Ile Asn Glu Ser Pro 
225                 230                 235                 240 


Val Leu Ser Tyr Met Asp Leu Val Gly Phe Ser Tyr Gln Val Ala Asn 
                245                 250                 255     


Gly Met Glu Phe Leu Ala Ser Lys Asn Cys Val His Arg Asp Leu Ala 
            260                 265                 270         


Ala Arg Asn Val Leu Ile Cys Glu Gly Lys Leu Val Lys Ile Cys Asp 
        275                 280                 285             


Phe Gly Leu Ala Arg Asp Ile Met Arg Asp Ser Asn Tyr Ile Ser Lys 
    290                 295                 300                 


Gly Ser Thr Phe Leu Pro Leu Lys Trp Met Ala Pro Glu Ser Ile Phe 
305                 310                 315                 320 


Asn Ser Leu Tyr Thr Thr Leu Ser Asp Val Trp Ser Phe Gly Ile Leu 
                325                 330                 335     


Leu Trp Glu Ile Phe Thr Leu Gly Gly Thr Pro Tyr Pro Glu Leu Pro 
            340                 345                 350         


Met Asn Glu Gln Phe Tyr Asn Ala Ile Lys Arg Gly Tyr Arg Met Ala 
        355                 360                 365             


Gln Pro Ala His Ala Ser Asp Glu Ile Tyr Glu Ile Met Gln Lys Cys 
    370                 375                 380                 


Trp Glu Glu Lys Phe Glu Ile Arg Pro Pro Phe Ser Gln Leu Val Leu 
385                 390                 395                 400 


Leu Leu Glu Arg Leu Leu Gly Glu Gly Tyr Lys Lys Lys Tyr Gln Gln 
                405                 410                 415     


Val Asp Glu Glu Phe Leu Arg Ser Asp His Pro Ala Ile Leu Arg Ser 
            420                 425                 430         


Gln Ala Arg Leu Pro Gly Phe His Gly Leu Arg Ser Pro Leu Asp Thr 
        435                 440                 445             


Ser Ser Val Leu Tyr Thr Ala Val Gln Pro Asn Glu Gly Asp Asn Asp 
    450                 455                 460                 


Tyr Ile Ile Pro Leu Pro Asp Pro Lys Pro Glu Val Ala Asp Glu Gly 
465                 470                 475                 480 


Pro Leu Glu Gly Ser Pro Ser Leu Ala Ser Ser Thr Leu Asn Glu Val 
                485                 490                 495     


Asn Thr Ser Ser Thr Ile Ser Cys Asp Ser Pro Leu Glu Pro Gln Asp 
            500                 505                 510         


Glu Pro Glu Pro Glu Pro Gln Leu Glu Leu Gln Val Glu Pro Glu Pro 
        515                 520                 525             


Glu Leu Glu Gln Leu Pro Asp Ser Gly Cys Pro Ala Pro Arg Ala Glu 
    530                 535                 540                 


Ala Glu Asp Ser Phe Leu 
545                 550 


