                               SEQUENCE LISTING

<110> CIBUS US LLC
      CIBUS EUROPE B.V.
 
<120> METHODS AND COMPOSITIONS FOR INCREASING EFFICIENCY OF INCREASED 
      EFFICIENCY OF TARGETED GENE MODIFICATION USING 
      OLIGONUCLEOTIDE-MEDIATED GENE REPAIR

<130> CIBUS-024-PCT

<140> PCT/US2014/029566
<141> 2014-03-14

<150> 61/801,333
<151> 2013-03-15

<160> 38    

<170> PatentIn version 3.5

<210> 1
<211> 99
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> misc_feature
<222> (1)..(4)
<223> Phosphothioate linkage between nucleotides

<220>
<221> misc_feature
<222> (96)..(99)
<223> Phosphothioate linkage between nucleotides

<400> 1
gtcgtgctgc ttcatgtggc ggggtagcgg ctgaagcact gcacgccgta ggtgaaggtg       60

gtcacgaggg tgggccaggc acgggcagct tgccggtgg                              99


<210> 2
<211> 101
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> misc_feature
<222> (1)..(4)
<223> Phosphothioate linkage between nucleotides

<220>
<221> misc_feature
<222> (98)..(101)
<223> Phosphothioate linkage between nucleotides

<400> 2
gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt gggtgaaggt       60

ggtcacgagg gtgggccagg gcacgggcag cttgccggtg g                          101


<210> 3
<211> 101
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> misc_feature
<222> (1)..(4)
<223> Phosphothioate linkage between nucleotides

<220>
<221> misc_feature
<222> (98)..(101)
<223> Phosphothioate linkage between nucleotides

<400> 3
ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcacc tacggcgtgc       60

agtgcttcag ccgctacccc gaccacatga agcagcacga c                          101


<210> 4
<211> 101
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> misc_feature
<222> (1)..(4)
<223> Phosphothioate linkage between nucleotides

<220>
<221> misc_feature
<222> (98)..(101)
<223> Phosphothioate linkage between nucleotides

<400> 4
ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcacc cacggcgtgc       60

agtgcttcag ccgctacccc gaccacatga agcagcacga c                          101


<210> 5
<211> 201
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> misc_feature
<222> (1)..(4)
<223> Phosphothioate linkage between nucleotides

<220>
<221> misc_feature
<222> (198)..(201)
<223> Phosphothioate linkage between nucleotides

<400> 5
aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc       60

ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtgaaggt ggtcacgagg      120

gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg      180

taggtggcat cgccctcgcc c                                                201


<210> 6
<211> 201
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> misc_feature
<222> (1)..(4)
<223> Phosphothioate linkage between nucleotides

<220>
<221> misc_feature
<222> (198)..(201)
<223> Phosphothioate linkage between nucleotides

<400> 6
aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc       60

ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt gggtgaaggt ggtcacgagg      120

gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg      180

taggtggcat cgccctcgcc c                                                201


<210> 7
<211> 201
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> misc_feature
<222> (1)..(4)
<223> Phosphothioate linkage between nucleotides

<220>
<221> misc_feature
<222> (198)..(201)
<223> Phosphothioate linkage between nucleotides

<400> 7
gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa       60

gctgcccgtg ccctggccca ccctcgtgac caccttcacc tacggcgtgc agtgcttcag      120

ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta      180

cgtccaggag cgcaccatct t                                                201


<210> 8
<211> 201
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> misc_feature
<222> (1)..(4)
<223> Phosphothioate linkage between nucleotides

<220>
<221> misc_feature
<222> (198)..(201)
<223> Phosphothioate linkage between nucleotides

<400> 8
gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa       60

gctgcccgtg ccctggccca ccctcgtgac caccttcacc cacggcgtgc agtgcttcag      120

ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta      180

cgtccaggag cgcaccatct t                                                201


<210> 9
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Pro Lys Lys Arg Lys Val 
1               5       


<210> 10
<211> 110
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> modified_base
<222> (1)..(20)
<223> a, c, t, g, unknown or other

<400> 10
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatgttc ttgaaaaaag tgagtggcac cgagtcggtg gtgctttttt                 110


<210> 11
<211> 48
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (3)..(32)
<223> a, c, u, g, unknown or other

<400> 11
acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnguuuuaga gcuaugcu                    48


<210> 12
<211> 67
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide

<400> 12
agcauagcaa guuaaaauaa ggctaguccg uuaucaacuu gaaaaagugg caccgagucg       60

gugcuuu                                                                 67


<210> 13
<211> 62
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(20)
<223> a, c, u, g, unknown or other

<400> 13
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cg                                                                      62


<210> 14
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 14
tcgtgaccac cttcacccac ggc                                               23


<210> 15
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 15
Gly Val Thr Thr Phe Thr Tyr 
1               5           


<210> 16
<211> 84
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 16
gtggaaagga cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag       60

ttaaaataag gctagtccgt tttt                                              84


<210> 17
<211> 84
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 17
aaaaacggac tagccttatt ttaacttgct atttctagct ctaaaacagg tcttctcgaa       60

gacccggtgt ttcgtccttt ccac                                              84


<210> 18
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (6)..(24)
<223> a, c, t, g, unknown or other

<400> 18
caccgnnnnn nnnnnnnnnn nnnn                                              24


<210> 19
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (5)..(23)
<223> a, c, t, g, unknown or other

<400> 19
aaacnnnnnn nnnnnnnnnn nnnc                                              24


<210> 20
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 20
Thr Ala Met Arg Pro Leu Thr Val Ala Ala Val 
1               5                   10      


<210> 21
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 21
actgcaatgc ggccattgac agcagctgtt actgctgctg g                           41


<210> 22
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 22
ccgctgccgt tactgctgca                                                   20


<210> 23
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 23
cggctgcagt tactgctgct                                                   20


<210> 24
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 24
ccgctgcagt tactgctgca                                                   20


<210> 25
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 25
ccgctgcagt tacagctgca                                                   20


<210> 26
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 26
cagctgctgt aacagccgct                                                   20


<210> 27
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 27
cagcagcagt tgctgtagct                                                   20


<210> 28
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 28
cagcagcagt tacagtagct                                                   20


<210> 29
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 29
tgcgcctcgc tttgtcttgt                                                   20


<210> 30
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 30
attttacagg tgtttacgcc                                                   20


<210> 31
<211> 71
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> 2'-O-Me modified nucleotide

<220>
<221> modified_base
<222> (11)..(71)
<223> 2'-O-Me modified nucleotide

<400> 31
uucauguggu cggggtagcg gctgaagcac tgcacgccgt aggtgaaggt ggtcacgagg       60

gtgggccagg g                                                            71


<210> 32
<211> 71
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> 2'-O-Me modified nucleotide

<220>
<221> modified_base
<222> (11)..(71)
<223> 2'-O-Me modified nucleotide

<400> 32
uucauguggu cggggtagcg gctgaagcac tgcacgccgt gggtgaaggt ggtcacgagg       60

gtgggccagg g                                                            71


<210> 33
<211> 71
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> 2'-O-Me modified nucleotide

<220>
<221> modified_base
<222> (11)..(71)
<223> 2'-O-Me modified nucleotide

<400> 33
gcugcccgug ccctggccca ccctcgtgac caccttcacc tacggcgtgc agtgcttcag       60

ccgctacccc g                                                            71


<210> 34
<211> 71
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> 2'-O-Me modified nucleotide

<220>
<221> modified_base
<222> (11)..(71)
<223> 2'-O-Me modified nucleotide

<400> 34
gcugcccgug ccctggccca ccctcgtgac caccttcacc cacggcgtgc agtgcttcag       60

ccgctacccc g                                                            71


<210> 35
<211> 71
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(9)
<223> 2'-O-Me modified nucleotide

<220>
<221> modified_base
<222> (11)..(71)
<223> 2'-O-Me modified nucleotide

<400> 35
uucauguggu cggggtagcg gctgaagcac tgcacgccgt aggtgaaggt ggtcacgagg       60

gtgggccagg g                                                            71


<210> 36
<211> 71
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(9)
<223> 2'-O-Me modified nucleotide

<220>
<221> modified_base
<222> (11)..(71)
<223> 2'-O-Me modified nucleotide

<400> 36
uucauguggu cggggtagcg gctgaagcac tgcacgccgt gggtgaaggt ggtcacgagg       60

gtgggccagg g                                                            71


<210> 37
<211> 71
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(9)
<223> 2'-O-Me modified nucleotide

<220>
<221> modified_base
<222> (11)..(71)
<223> 2'-O-Me modified nucleotide

<400> 37
gcugcccgug ccctggccca ccctcgtgac caccttcacc tacggcgtgc agtgcttcag       60

ccgctacccc g                                                            71


<210> 38
<211> 71
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(9)
<223> 2'-O-Me modified nucleotide

<220>
<221> modified_base
<222> (11)..(71)
<223> 2'-O-Me modified nucleotide

<400> 38
gcugcccgug ccctggccca ccctcgtgac caccttcacc cacggcgtgc agtgcttcag       60

ccgctacccc g                                                            71


