                         SEQUENCE LISTING

<110>  CITY OF HOPE
       FORMAN, Stephen
       MARDIROS, Armen
 
<120>  CD123-SPECIFIC CHIMERIC ANTIGEN RECEPTOR REDIRECTED T CELLS AND 
       METHODS OF THEIR USE

<130>  54435.8127.WO00

<150>  US 13/844,048
<151>  2013-03-15

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  2052
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense nucleotide sequence of the 32716CAR(S228P+L235E) 
       construct

<400>  1
gctagcgccg ccaccatgct gctgctggtg accagcctgc tgctgtgcga gctgccccac       60

cccgcctttc tgctgatccc ccagattcag ctggtgcaga gcggcccgga actgaaaaaa      120

ccgggcgaaa ccgtgaaaat tagctgcaaa gcgagcggct atatttttac caactatggc      180

atgaactggg tgaaacaggc gccgggcaaa agctttaaat ggatgggctg gattaacacc      240

tataccggcg aaagcaccta tagcgcggat tttaaaggcc gctttgcgtt tagcctggaa      300

accagcgcga gcaccgcgta tctgcatatt aacgatctga aaaacgaaga taccgcgacc      360

tatttttgcg cgcgcagcgg cggctatgat ccgatggatt attggggcca gggcaccagc      420

gtgaccgtga gcagcggcgg cggcggcagc ggcggcggcg gcagcggcgg cggcggcagc      480

gatattgtgc tgacccagag cccggcgagc ctggcggtga gcctgggcca gcgcgcgacc      540

attagctgcc gcgcgagcga aagcgtggat aactatggca acacctttat gcattggtat      600

cagcagaaac cgggccagcc gccgaaactg ctgatttatc gcgcgagcaa cctggaaagc      660

ggcattccgg cgcgctttag cggcagcggc agccgcaccg attttaccct gaccattaac      720

ccggtggaag cggatgatgt ggcgacctat tattgccagc agagcaacga agatccgccg      780

acctttggcg cgggcaccaa actggaactg aaagagagca agtacggccc tccctgcccc      840

ccttgccctg cccccgagtt cgagggcgga cccagcgtgt tcctgttccc ccccaagccc      900

aaggacaccc tgatgatcag ccggaccccc gaggtgacct gcgtggtggt ggacgtgagc      960

caggaagatc ccgaggtcca gttcaattgg tacgtggacg gcgtggaagt gcacaacgcc     1020

aagaccaagc ccagagagga acagttcaac agcacctacc gggtggtgtc tgtgctgacc     1080

gtgctgcacc aggactggct gaacggcaaa gaatacaagt gcaaggtgtc caacaagggc     1140

ctgcccagca gcatcgaaaa gaccatcagc aaggccaagg gccagcctcg cgagccccag     1200

gtgtacaccc tgcctccctc ccaggaagag atgaccaaga accaggtgtc cctgacctgc     1260

ctggtgaagg gcttctaccc cagcgacatc gccgtggagt gggagagcaa cggccagcct     1320

gagaacaact acaagaccac ccctcccgtg ctggacagcg acggcagctt cttcctgtac     1380

agccggctga ccgtggacaa gagccggtgg caggaaggca acgtctttag ctgcagcgtg     1440

atgcacgagg ccctgcacaa ccactacacc cagaagagcc tgagcctgtc cctgggcaag     1500

atgttctggg tgctggtggt ggtgggcggg gtgctggcct gctacagcct gctggtgaca     1560

gtggccttca tcatcttttg ggtgcggagc aagcggagca gaggcggcca cagcgactac     1620

atgaacatga cccccagacg gcctggcccc acccggaagc actaccagcc ctacgcccca     1680

cccagggact ttgccgccta ccggtccggc ggagggcggg tgaagttcag cagaagcgcc     1740

gacgcccctg cctaccagca gggccagaat cagctgtaca acgagctgaa cctgggcaga     1800

agggaagagt acgacgtcct ggataagcgg agaggccggg accctgagat gggcggcaag     1860

cctcggcgga agaaccccca ggaaggcctg tataacgaac tgcagaaaga caagatggcc     1920

gaggcctaca gcgagatcgg catgaagggc gagcggaggc ggggcaaggg ccacgacggc     1980

ctgtatcagg gcctgtccac cgccaccaag gatacctacg acgccctgca catgcaggcc     2040

ctgcccccaa gg                                                         2052


<210>  2
<211>  2031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense nucleotide sequence of the 26292CAR(S228P+L235E) 
       construct

<400>  2
gctagcgccg ccaccatgct gctgctggtg accagcctgc tgctgtgcga gctgccccac       60

cccgcctttc tgctgatccc ccaggtgcag ctgcagcagc cgggcgcgga actggtgcgc      120

ccgggcgcga gcgtgaaact gagctgcaaa gcgagcggct atacctttac cagctattgg      180

atgaactggg tgaaacagcg cccggatcag ggcctggaat ggattggccg cattgatccg      240

tatgatagcg aaacccatta taaccagaaa tttaaagata aagcgattct gaccgtggat      300

aaaagcagca gcaccgcgta tatgcagctg agcagcctga ccagcgaaga tagcgcggtg      360

tattattgcg cgcgcggcaa ctgggatgat tattggggcc agggcaccac cctgaccgtg      420

agcagcggcg gcggcggcag cggcggcggc ggcagcggcg gcggcggcag cgatgtgcag      480

attacccaga gcccgagcta tctggcggcg agcccgggcg aaaccattac cattaactgc      540

cgcgcgagca aaagcattag caaagatctg gcgtggtatc aggaaaaacc gggcaaaacc      600

aacaaactgc tgatttatag cggcagcacc ctgcagagcg gcattccgag ccgctttagc      660

ggcagcggca gcggcaccga ttttaccctg accattagca gcctggaacc ggaagatttt      720

gcgatgtatt attgccagca gcataacaaa tatccgtata cctttggcgg cggcaccaaa      780

ctggaaatta aagagagcaa gtacggccct ccctgccccc cttgccctgc ccccgagttc      840

gagggcggac ccagcgtgtt cctgttcccc cccaagccca aggacaccct gatgatcagc      900

cggacccccg aggtgacctg cgtggtggtg gacgtgagcc aggaagatcc cgaggtccag      960

ttcaattggt acgtggacgg cgtggaagtg cacaacgcca agaccaagcc cagagaggaa     1020

cagttcaaca gcacctaccg ggtggtgtct gtgctgaccg tgctgcacca ggactggctg     1080

aacggcaaag aatacaagtg caaggtgtcc aacaagggcc tgcccagcag catcgaaaag     1140

accatcagca aggccaaggg ccagcctcgc gagccccagg tgtacaccct gcctccctcc     1200

caggaagaga tgaccaagaa ccaggtgtcc ctgacctgcc tggtgaaggg cttctacccc     1260

agcgacatcg ccgtggagtg ggagagcaac ggccagcctg agaacaacta caagaccacc     1320

cctcccgtgc tggacagcga cggcagcttc ttcctgtaca gccggctgac cgtggacaag     1380

agccggtggc aggaaggcaa cgtctttagc tgcagcgtga tgcacgaggc cctgcacaac     1440

cactacaccc agaagagcct gagcctgtcc ctgggcaaga tgttctgggt gctggtggtg     1500

gtgggcgggg tgctggcctg ctacagcctg ctggtgacag tggccttcat catcttttgg     1560

gtgcggagca agcggagcag aggcggccac agcgactaca tgaacatgac ccccagacgg     1620

cctggcccca cccggaagca ctaccagccc tacgccccac ccagggactt tgccgcctac     1680

cggtccggcg gagggcgggt gaagttcagc agaagcgccg acgcccctgc ctaccagcag     1740

ggccagaatc agctgtacaa cgagctgaac ctgggcagaa gggaagagta cgacgtcctg     1800

gataagcgga gaggccggga ccctgagatg ggcggcaagc ctcggcggaa gaacccccag     1860

gaaggcctgt ataacgaact gcagaaagac aagatggccg aggcctacag cgagatcggc     1920

atgaagggcg agcggaggcg gggcaagggc cacgacggcc tgtatcaggg cctgtccacc     1980

gccaccaagg atacctacga cgccctgcac atgcaggccc tgcccccaag g              2031


<210>  3
<211>  2052
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense nucleotide sequence of the 32716CAR(S228P+L235E+N297Q) 
       construct

<400>  3
gctagcgccg ccaccatgct gctgctggtg accagcctgc tgctgtgcga gctgccccac       60

cccgcctttc tgctgatccc ccagattcag ctggtgcaga gcggcccgga actgaaaaaa      120

ccgggcgaaa ccgtgaaaat tagctgcaaa gcgagcggct atatttttac caactatggc      180

atgaactggg tgaaacaggc gccgggcaaa agctttaaat ggatgggctg gattaacacc      240

tataccggcg aaagcaccta tagcgcggat tttaaaggcc gctttgcgtt tagcctggaa      300

accagcgcga gcaccgcgta tctgcatatt aacgatctga aaaacgaaga taccgcgacc      360

tatttttgcg cgcgcagcgg cggctatgat ccgatggatt attggggcca gggcaccagc      420

gtgaccgtga gcagcggcgg cggcggcagc ggcggcggcg gcagcggcgg cggcggcagc      480

gatattgtgc tgacccagag cccggcgagc ctggcggtga gcctgggcca gcgcgcgacc      540

attagctgcc gcgcgagcga aagcgtggat aactatggca acacctttat gcattggtat      600

cagcagaaac cgggccagcc gccgaaactg ctgatttatc gcgcgagcaa cctggaaagc      660

ggcattccgg cgcgctttag cggcagcggc agccgcaccg attttaccct gaccattaac      720

ccggtggaag cggatgatgt ggcgacctat tattgccagc agagcaacga agatccgccg      780

acctttggcg cgggcaccaa actggaactg aaagagagca agtacggccc tccctgcccc      840

ccttgccctg cccccgagtt cgagggcgga cccagcgtgt tcctgttccc ccccaagccc      900

aaggacaccc tgatgatcag ccggaccccc gaggtgacct gcgtggtggt ggacgtgagc      960

caggaagatc ccgaggtcca gttcaattgg tacgtggacg gcgtggaagt gcacaacgcc     1020

aagaccaagc ccagagagga acagttccar agcacctacc gggtggtgtc tgtgctgacc     1080

gtgctgcacc aggactggct gaacggcaaa gaatacaagt gcaaggtgtc caacaagggc     1140

ctgcccagca gcatcgaaaa gaccatcagc aaggccaagg gccagcctcg cgagccccag     1200

gtgtacaccc tgcctccctc ccaggaagag atgaccaaga accaggtgtc cctgacctgc     1260

ctggtgaagg gcttctaccc cagcgacatc gccgtggagt gggagagcaa cggccagcct     1320

gagaacaact acaagaccac ccctcccgtg ctggacagcg acggcagctt cttcctgtac     1380

agccggctga ccgtggacaa gagccggtgg caggaaggca acgtctttag ctgcagcgtg     1440

atgcacgagg ccctgcacaa ccactacacc cagaagagcc tgagcctgtc cctgggcaag     1500

atgttctggg tgctggtggt ggtgggcggg gtgctggcct gctacagcct gctggtgaca     1560

gtggccttca tcatcttttg ggtgcggagc aagcggagca gaggcggcca cagcgactac     1620

atgaacatga cccccagacg gcctggcccc acccggaagc actaccagcc ctacgcccca     1680

cccagggact ttgccgccta ccggtccggc ggagggcggg tgaagttcag cagaagcgcc     1740

gacgcccctg cctaccagca gggccagaat cagctgtaca acgagctgaa cctgggcaga     1800

agggaagagt acgacgtcct ggataagcgg agaggccggg accctgagat gggcggcaag     1860

cctcggcgga agaaccccca ggaaggcctg tataacgaac tgcagaaaga caagatggcc     1920

gaggcctaca gcgagatcgg catgaagggc gagcggaggc ggggcaaggg ccacgacggc     1980

ctgtatcagg gcctgtccac cgccaccaag gatacctacg acgccctgca catgcaggcc     2040

ctgcccccaa gg                                                         2052


<210>  4
<211>  2031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antisense nucleotide sequence of the 26292CAR(S228P+L235E+N297Q) 
       construct

<400>  4
gctagcgccg ccaccatgct gctgctggtg accagcctgc tgctgtgcga gctgccccac       60

cccgcctttc tgctgatccc ccaggtgcag ctgcagcagc cgggcgcgga actggtgcgc      120

ccgggcgcga gcgtgaaact gagctgcaaa gcgagcggct atacctttac cagctattgg      180

atgaactggg tgaaacagcg cccggatcag ggcctggaat ggattggccg cattgatccg      240

tatgatagcg aaacccatta taaccagaaa tttaaagata aagcgattct gaccgtggat      300

aaaagcagca gcaccgcgta tatgcagctg agcagcctga ccagcgaaga tagcgcggtg      360

tattattgcg cgcgcggcaa ctgggatgat tattggggcc agggcaccac cctgaccgtg      420

agcagcggcg gcggcggcag cggcggcggc ggcagcggcg gcggcggcag cgatgtgcag      480

attacccaga gcccgagcta tctggcggcg agcccgggcg aaaccattac cattaactgc      540

cgcgcgagca aaagcattag caaagatctg gcgtggtatc aggaaaaacc gggcaaaacc      600

aacaaactgc tgatttatag cggcagcacc ctgcagagcg gcattccgag ccgctttagc      660

ggcagcggca gcggcaccga ttttaccctg accattagca gcctggaacc ggaagatttt      720

gcgatgtatt attgccagca gcataacaaa tatccgtata cctttggcgg cggcaccaaa      780

ctggaaatta aagagagcaa gtacggccct ccctgccccc cttgccctgc ccccgagttc      840

gagggcggac ccagcgtgtt cctgttcccc cccaagccca aggacaccct gatgatcagc      900

cggacccccg aggtgacctg cgtggtggtg gacgtgagcc aggaagatcc cgaggtccag      960

ttcaattggt acgtggacgg cgtggaagtg cacaacgcca agaccaagcc cagagaggaa     1020

cagttcaara gcacctaccg ggtggtgtct gtgctgaccg tgctgcacca ggactggctg     1080

aacggcaaag aatacaagtg caaggtgtcc aacaagggcc tgcccagcag catcgaaaag     1140

accatcagca aggccaaggg ccagcctcgc gagccccagg tgtacaccct gcctccctcc     1200

caggaagaga tgaccaagaa ccaggtgtcc ctgacctgcc tggtgaaggg cttctacccc     1260

agcgacatcg ccgtggagtg ggagagcaac ggccagcctg agaacaacta caagaccacc     1320

cctcccgtgc tggacagcga cggcagcttc ttcctgtaca gccggctgac cgtggacaag     1380

agccggtggc aggaaggcaa cgtctttagc tgcagcgtga tgcacgaggc cctgcacaac     1440

cactacaccc agaagagcct gagcctgtcc ctgggcaaga tgttctgggt gctggtggtg     1500

gtgggcgggg tgctggcctg ctacagcctg ctggtgacag tggccttcat catcttttgg     1560

gtgcggagca agcggagcag aggcggccac agcgactaca tgaacatgac ccccagacgg     1620

cctggcccca cccggaagca ctaccagccc tacgccccac ccagggactt tgccgcctac     1680

cggtccggcg gagggcgggt gaagttcagc agaagcgccg acgcccctgc ctaccagcag     1740

ggccagaatc agctgtacaa cgagctgaac ctgggcagaa gggaagagta cgacgtcctg     1800

gataagcgga gaggccggga ccctgagatg ggcggcaagc ctcggcggaa gaacccccag     1860

gaaggcctgt ataacgaact gcagaaagac aagatggccg aggcctacag cgagatcggc     1920

atgaagggcg agcggaggcg gggcaagggc cacgacggcc tgtatcaggg cctgtccacc     1980

gccaccaagg atacctacga cgccctgcac atgcaggccc tgcccccaag g              2031


<210>  5
<211>  2052
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sense nucleotide sequence of the 32716CAR(S228P+L235E) construct

<400>  5
cgatcgcggc ggtggtacga cgacgaccac tggtcggacg acgacacgct cgacggggtg       60

gggcggaaag acgactaggg ggtctaagtc gaccacgtct cgccgggcct tgactttttt      120

ggcccgcttt ggcactttta atcgacgttt cgctcgccga tataaaaatg gttgataccg      180

tacttgaccc actttgtccg cggcccgttt tcgaaattta cctacccgac ctaattgtgg      240

atatggccgc tttcgtggat atcgcgccta aaatttccgg cgaaacgcaa atcggacctt      300

tggtcgcgct cgtggcgcat agacgtataa ttgctagact ttttgcttct atggcgctgg      360

ataaaaacgc gcgcgtcgcc gccgatacta ggctacctaa taaccccggt cccgtggtcg      420

cactggcact cgtcgccgcc gccgccgtcg ccgccgccgc cgtcgccgcc gccgccgtcg      480

ctataacacg actgggtctc gggccgctcg gaccgccact cggacccggt cgcgcgctgg      540

taatcgacgg cgcgctcgct ttcgcaccta ttgataccgt tgtggaaata cgtaaccata      600

gtcgtctttg gcccggtcgg cggctttgac gactaaatag cgcgctcgtt ggacctttcg      660

ccgtaaggcc gcgcgaaatc gccgtcgccg tcggcgtggc taaaatggga ctggtaattg      720

ggccaccttc gcctactaca ccgctggata ataacggtcg tctcgttgct tctaggcggc      780

tggaaaccgc gcccgtggtt tgaccttgac tttctctcgt tcatgccggg agggacgggg      840

ggaacgggac gggggctcaa gctcccgcct gggtcgcaca aggacaaggg ggggttcggg      900

ttcctgtggg actactagtc ggcctggggg ctccactgga cgcaccacca cctgcactcg      960

gtccttctag ggctccaggt caagttaacc atgcacctgc cgcaccttca cgtgttgcgg     1020

ttctggttcg ggtctctcct tgtcaagttg tcgtggatgg cccaccacag acacgactgg     1080

cacgacgtgg tcctgaccga cttgccgttt cttatgttca cgttccacag gttgttcccg     1140

gacgggtcgt cgtagctttt ctggtagtcg ttccggttcc cggtcggagc gctcggggtc     1200

cacatgtggg acggagggag ggtccttctc tactggttct tggtccacag ggactggacg     1260

gaccacttcc cgaagatggg gtcgctgtag cggcacctca ccctctcgtt gccggtcgga     1320

ctcttgttga tgttctggtg gggagggcac gacctgtcgc tgccgtcgaa gaaggacatg     1380

tcggccgact ggcacctgtt ctcggccacc gtccttccgt tgcagaaatc gacgtcgcac     1440

tacgtgctcc gggacgtgtt ggtgatgtgg gtcttctcgg actcggacag ggacccgttc     1500

tacaagaccc acgaccacca ccacccgccc cacgaccgga cgatgtcgga cgaccactgt     1560

caccggaagt agtagaaaac ccacgcctcg ttcgcctcgt ctccgccggt gtcgctgatg     1620

tacttgtact gggggtctgc cggaccgggg tgggccttcg tgatggtcgg gatgcggggt     1680

gggtccctga aacggcggat ggccaggccg cctcccgccc acttcaagtc gtcttcgcgg     1740

ctgcggggac ggatggtcgt cccggtctta gtcgacatgt tgctcgactt ggacccgtct     1800

tcccttctca tgctgcagga cctattcgcc tctccggccc tgggactcta cccgccgttc     1860

ggagccgcct tcttgggggt ccttccggac atattgcttg acgtctttct gttctaccgg     1920

ctccggatgt cgctctagcc gtacttcccg ctcgcctccg ccccgttccc ggtgctgccg     1980

gacatagtcc cggacaggtg gcggtggttc ctatggatgc tgcgggacgt gtacgtccgg     2040

gacgggggtt cc                                                         2052


<210>  6
<211>  2031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sense nucleotide sequence of the 26292CAR(S228P+L235E) construct

<400>  6
cgatcgcggc ggtggtacga cgacgaccac tggtcggacg acgacacgct cgacggggtg       60

gggcggaaag acgactaggg ggtccacgtc gacgtcgtcg gcccgcgcct tgaccacgcg      120

ggcccgcgct cgcactttga ctcgacgttt cgctcgccga tatggaaatg gtcgataacc      180

tacttgaccc actttgtcgc gggcctagtc ccggacctta cctaaccggc gtaactaggc      240

atactatcgc tttgggtaat attggtcttt aaatttctat ttcgctaaga ctggcaccta      300

ttttcgtcgt cgtggcgcat atacgtcgac tcgtcggact ggtcgcttct atcgcgccac      360

ataataacgc gcgcgccgtt gaccctacta ataaccccgg tcccgtggtg ggactggcac      420

tcgtcgccgc cgccgccgtc gccgccgccg ccgtcgccgc cgccgccgtc gctacacgtc      480

taatgggtct cgggctcgat agaccgccgc tcgggcccgc tttggtaatg gtaattgacg      540

gcgcgctcgt tttcgtaatc gtttctagac cgcaccatag tcctttttgg cccgttttgg      600

ttgtttgacg actaaatatc gccgtcgtgg gacgtctcgc cgtaaggctc ggcgaaatcg      660

ccgtcgccgt cgccgtggct aaaatgggac tggtaatcgt cggaccttgg ccttctaaaa      720

cgctacataa taacggtcgt cgtattgttt ataggcatat ggaaaccgcc gccgtggttt      780

gacctttaat ttctctcgtt catgccggga gggacggggg gaacgggacg ggggctcaag      840

ctcccgcctg ggtcgcacaa ggacaagggg gggttcgggt tcctgtggga ctactagtcg      900

gcctgggggc tccactggac gcaccaccac ctgcactcgg tccttctagg gctccaggtc      960

aagttaacca tgcacctgcc gcaccttcac gtgttgcggt tctggttcgg gtctctcctt     1020

gtcaagttgt cgtggatggc ccaccacaga cacgactggc acgacgtggt cctgaccgac     1080

ttgccgtttc ttatgttcac gttccacagg ttgttcccgg acgggtcgtc gtagcttttc     1140

tggtagtcgt tccggttccc ggtcggagcg ctcggggtcc acatgtggga cggagggagg     1200

gtccttctct actggttctt ggtccacagg gactggacgg accacttccc gaagatgggg     1260

tcgctgtagc ggcacctcac cctctcgttg ccggtcggac tcttgttgat gttctggtgg     1320

ggagggcacg acctgtcgct gccgtcgaag aaggacatgt cggccgactg gcacctgttc     1380

tcggccaccg tccttccgtt gcagaaatcg acgtcgcact acgtgctccg ggacgtgttg     1440

gtgatgtggg tcttctcgga ctcggacagg gacccgttct acaagaccca cgaccaccac     1500

cacccgcccc acgaccggac gatgtcggac gaccactgtc accggaagta gtagaaaacc     1560

cacgcctcgt tcgcctcgtc tccgccggtg tcgctgatgt acttgtactg ggggtctgcc     1620

ggaccggggt gggccttcgt gatggtcggg atgcggggtg ggtccctgaa acggcggatg     1680

gccaggccgc ctcccgccca cttcaagtcg tcttcgcggc tgcggggacg gatggtcgtc     1740

ccggtcttag tcgacatgtt gctcgacttg gacccgtctt cccttctcat gctgcaggac     1800

ctattcgcct ctccggccct gggactctac ccgccgttcg gagccgcctt cttgggggtc     1860

cttccggaca tattgcttga cgtctttctg ttctaccggc tccggatgtc gctctagccg     1920

tacttcccgc tcgcctccgc cccgttcccg gtgctgccgg acatagtccc ggacaggtgg     1980

cggtggttcc tatggatgct gcgggacgtg tacgtccggg acgggggttc c              2031


<210>  7
<211>  2052
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sense nucleotide sequence of the 32716CAR(S228P+L235E+N297Q) 
       construct

<400>  7
cgatcgcggc ggtggtacga cgacgaccac tggtcggacg acgacacgct cgacggggtg       60

gggcggaaag acgactaggg ggtctaagtc gaccacgtct cgccgggcct tgactttttt      120

ggcccgcttt ggcactttta atcgacgttt cgctcgccga tataaaaatg gttgataccg      180

tacttgaccc actttgtccg cggcccgttt tcgaaattta cctacccgac ctaattgtgg      240

atatggccgc tttcgtggat atcgcgccta aaatttccgg cgaaacgcaa atcggacctt      300

tggtcgcgct cgtggcgcat agacgtataa ttgctagact ttttgcttct atggcgctgg      360

ataaaaacgc gcgcgtcgcc gccgatacta ggctacctaa taaccccggt cccgtggtcg      420

cactggcact cgtcgccgcc gccgccgtcg ccgccgccgc cgtcgccgcc gccgccgtcg      480

ctataacacg actgggtctc gggccgctcg gaccgccact cggacccggt cgcgcgctgg      540

taatcgacgg cgcgctcgct ttcgcaccta ttgataccgt tgtggaaata cgtaaccata      600

gtcgtctttg gcccggtcgg cggctttgac gactaaatag cgcgctcgtt ggacctttcg      660

ccgtaaggcc gcgcgaaatc gccgtcgccg tcggcgtggc taaaatggga ctggtaattg      720

ggccaccttc gcctactaca ccgctggata ataacggtcg tctcgttgct tctaggcggc      780

tggaaaccgc gcccgtggtt tgaccttgac tttctctcgt tcatgccggg agggacgggg      840

ggaacgggac gggggctcaa gctcccgcct gggtcgcaca aggacaaggg ggggttcggg      900

ttcctgtggg actactagtc ggcctggggg ctccactgga cgcaccacca cctgcactcg      960

gtccttctag ggctccaggt caagttaacc atgcacctgc cgcaccttca cgtgttgcgg     1020

ttctggttcg ggtctctcct tgtcaaggty tcgtggatgg cccaccacag acacgactgg     1080

cacgacgtgg tcctgaccga cttgccgttt cttatgttca cgttccacag gttgttcccg     1140

gacgggtcgt cgtagctttt ctggtagtcg ttccggttcc cggtcggagc gctcggggtc     1200

cacatgtggg acggagggag ggtccttctc tactggttct tggtccacag ggactggacg     1260

gaccacttcc cgaagatggg gtcgctgtag cggcacctca ccctctcgtt gccggtcgga     1320

ctcttgttga tgttctggtg gggagggcac gacctgtcgc tgccgtcgaa gaaggacatg     1380

tcggccgact ggcacctgtt ctcggccacc gtccttccgt tgcagaaatc gacgtcgcac     1440

tacgtgctcc gggacgtgtt ggtgatgtgg gtcttctcgg actcggacag ggacccgttc     1500

tacaagaccc acgaccacca ccacccgccc cacgaccgga cgatgtcgga cgaccactgt     1560

caccggaagt agtagaaaac ccacgcctcg ttcgcctcgt ctccgccggt gtcgctgatg     1620

tacttgtact gggggtctgc cggaccgggg tgggccttcg tgatggtcgg gatgcggggt     1680

gggtccctga aacggcggat ggccaggccg cctcccgccc acttcaagtc gtcttcgcgg     1740

ctgcggggac ggatggtcgt cccggtctta gtcgacatgt tgctcgactt ggacccgtct     1800

tcccttctca tgctgcagga cctattcgcc tctccggccc tgggactcta cccgccgttc     1860

ggagccgcct tcttgggggt ccttccggac atattgcttg acgtctttct gttctaccgg     1920

ctccggatgt cgctctagcc gtacttcccg ctcgcctccg ccccgttccc ggtgctgccg     1980

gacatagtcc cggacaggtg gcggtggttc ctatggatgc tgcgggacgt gtacgtccgg     2040

gacgggggtt cc                                                         2052


<210>  8
<211>  2031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sense nucleotide sequence of the 26292CAR(S228P+L235E+N297Q) 
       construct

<400>  8
cgatcgcggc ggtggtacga cgacgaccac tggtcggacg acgacacgct cgacggggtg       60

gggcggaaag acgactaggg ggtccacgtc gacgtcgtcg gcccgcgcct tgaccacgcg      120

ggcccgcgct cgcactttga ctcgacgttt cgctcgccga tatggaaatg gtcgataacc      180

tacttgaccc actttgtcgc gggcctagtc ccggacctta cctaaccggc gtaactaggc      240

atactatcgc tttgggtaat attggtcttt aaatttctat ttcgctaaga ctggcaccta      300

ttttcgtcgt cgtggcgcat atacgtcgac tcgtcggact ggtcgcttct atcgcgccac      360

ataataacgc gcgcgccgtt gaccctacta ataaccccgg tcccgtggtg ggactggcac      420

tcgtcgccgc cgccgccgtc gccgccgccg ccgtcgccgc cgccgccgtc gctacacgtc      480

taatgggtct cgggctcgat agaccgccgc tcgggcccgc tttggtaatg gtaattgacg      540

gcgcgctcgt tttcgtaatc gtttctagac cgcaccatag tcctttttgg cccgttttgg      600

ttgtttgacg actaaatatc gccgtcgtgg gacgtctcgc cgtaaggctc ggcgaaatcg      660

ccgtcgccgt cgccgtggct aaaatgggac tggtaatcgt cggaccttgg ccttctaaaa      720

cgctacataa taacggtcgt cgtattgttt ataggcatat ggaaaccgcc gccgtggttt      780

gacctttaat ttctctcgtt catgccggga gggacggggg gaacgggacg ggggctcaag      840

ctcccgcctg ggtcgcacaa ggacaagggg gggttcgggt tcctgtggga ctactagtcg      900

gcctgggggc tccactggac gcaccaccac ctgcactcgg tccttctagg gctccaggtc      960

aagttaacca tgcacctgcc gcaccttcac gtgttgcggt tctggttcgg gtctctcctt     1020

gtcaagttyt cgtggatggc ccaccacaga cacgactggc acgacgtggt cctgaccgac     1080

ttgccgtttc ttatgttcac gttccacagg ttgttcccgg acgggtcgtc gtagcttttc     1140

tggtagtcgt tccggttccc ggtcggagcg ctcggggtcc acatgtggga cggagggagg     1200

gtccttctct actggttctt ggtccacagg gactggacgg accacttccc gaagatgggg     1260

tcgctgtagc ggcacctcac cctctcgttg ccggtcggac tcttgttgat gttctggtgg     1320

ggagggcacg acctgtcgct gccgtcgaag aaggacatgt cggccgactg gcacctgttc     1380

tcggccaccg tccttccgtt gcagaaatcg acgtcgcact acgtgctccg ggacgtgttg     1440

gtgatgtggg tcttctcgga ctcggacagg gacccgttct acaagaccca cgaccaccac     1500

cacccgcccc acgaccggac gatgtcggac gaccactgtc accggaagta gtagaaaacc     1560

cacgcctcgt tcgcctcgtc tccgccggtg tcgctgatgt acttgtactg ggggtctgcc     1620

ggaccggggt gggccttcgt gatggtcggg atgcggggtg ggtccctgaa acggcggatg     1680

gccaggccgc ctcccgccca cttcaagtcg tcttcgcggc tgcggggacg gatggtcgtc     1740

ccggtcttag tcgacatgtt gctcgacttg gacccgtctt cccttctcat gctgcaggac     1800

ctattcgcct ctccggccct gggactctac ccgccgttcg gagccgcctt cttgggggtc     1860

cttccggaca tattgcttga cgtctttctg ttctaccggc tccggatgtc gctctagccg     1920

tacttcccgc tcgcctccgc cccgttcccg gtgctgccgg acatagtccc ggacaggtgg     1980

cggtggttcc tatggatgct gcgggacgtg tacgtccggg acgggggttc c              2031


<210>  9
<211>  679
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of the 32716CAR(S228P+L235E) construct

<400>  9

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Ile Gln Leu Val Gln Ser Gly Pro Glu 
            20                  25                  30          


Leu Lys Lys Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Ile Phe Thr Asn Tyr Gly Met Asn Trp Val Lys Gln Ala Pro Gly 
    50                  55                  60                  


Lys Ser Phe Lys Trp Met Gly Trp Ile Asn Thr Tyr Thr Gly Glu Ser 
65                  70                  75                  80  


Thr Tyr Ser Ala Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr 
                85                  90                  95      


Ser Ala Ser Thr Ala Tyr Leu His Ile Asn Asp Leu Lys Asn Glu Asp 
            100                 105                 110         


Thr Ala Thr Tyr Phe Cys Ala Arg Ser Gly Gly Tyr Asp Pro Met Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr 
145                 150                 155                 160 


Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile 
                165                 170                 175     


Ser Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr Gly Asn Thr Phe Met 
            180                 185                 190         


His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
        195                 200                 205             


Arg Ala Ser Asn Leu Glu Ser Gly Ile Pro Ala Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Arg Thr Asp Phe Thr Leu Thr Ile Asn Pro Val Glu Ala Asp 
225                 230                 235                 240 


Asp Val Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Glu Asp Pro Pro Thr 
                245                 250                 255     


Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Glu Ser Lys Tyr Gly Pro 
            260                 265                 270         


Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val 
        275                 280                 285             


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
    290                 295                 300                 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu 
305                 310                 315                 320 


Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
                325                 330                 335     


Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 
            340                 345                 350         


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
        355                 360                 365             


Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile 
    370                 375                 380                 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
385                 390                 395                 400 


Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
                405                 410                 415     


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
            420                 425                 430         


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
        435                 440                 445             


Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg 
    450                 455                 460                 


Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
465                 470                 475                 480 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met 
                485                 490                 495     


Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
            500                 505                 510         


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
        515                 520                 525             


Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
    530                 535                 540                 


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
545                 550                 555                 560 


Ala Tyr Arg Ser Gly Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp 
                565                 570                 575     


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
            580                 585                 590         


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
        595                 600                 605             


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
    610                 615                 620                 


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
625                 630                 635                 640 


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
                645                 650                 655     


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
            660                 665                 670         


Met Gln Ala Leu Pro Pro Arg 
        675                 


<210>  10
<211>  672
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of the 26292CAR(S228P+L235E) construct

<400>  10

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Gln Gln Pro Gly Ala Glu 
            20                  25                  30          


Leu Val Arg Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Ser Tyr Trp Met Asn Trp Val Lys Gln Arg Pro Asp 
    50                  55                  60                  


Gln Gly Leu Glu Trp Ile Gly Arg Ile Asp Pro Tyr Asp Ser Glu Thr 
65                  70                  75                  80  


His Tyr Asn Gln Lys Phe Lys Asp Lys Ala Ile Leu Thr Val Asp Lys 
                85                  90                  95      


Ser Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp 
            100                 105                 110         


Ser Ala Val Tyr Tyr Cys Ala Arg Gly Asn Trp Asp Asp Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Gln Ile Thr Gln Ser Pro 
145                 150                 155                 160 


Ser Tyr Leu Ala Ala Ser Pro Gly Glu Thr Ile Thr Ile Asn Cys Arg 
                165                 170                 175     


Ala Ser Lys Ser Ile Ser Lys Asp Leu Ala Trp Tyr Gln Glu Lys Pro 
            180                 185                 190         


Gly Lys Thr Asn Lys Leu Leu Ile Tyr Ser Gly Ser Thr Leu Gln Ser 
        195                 200                 205             


Gly Ile Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
    210                 215                 220                 


Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala Met Tyr Tyr Cys 
225                 230                 235                 240 


Gln Gln His Asn Lys Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu 
                245                 250                 255     


Glu Ile Lys Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala 
            260                 265                 270         


Pro Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
        275                 280                 285             


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
    290                 295                 300                 


Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val 
305                 310                 315                 320 


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
                325                 330                 335     


Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
            340                 345                 350         


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly 
        355                 360                 365             


Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
    370                 375                 380                 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr 
385                 390                 395                 400 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
                405                 410                 415     


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
            420                 425                 430         


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
        435                 440                 445             


Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe 
    450                 455                 460                 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
465                 470                 475                 480 


Ser Leu Ser Leu Ser Leu Gly Lys Met Phe Trp Val Leu Val Val Val 
                485                 490                 495     


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
            500                 505                 510         


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr 
        515                 520                 525             


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
    530                 535                 540                 


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly Gly 
545                 550                 555                 560 


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
                565                 570                 575     


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            580                 585                 590         


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        595                 600                 605             


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    610                 615                 620                 


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
625                 630                 635                 640 


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                645                 650                 655     


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            660                 665                 670         


<210>  11
<211>  679
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of the 32716CAR(S228P+L235E+N297Q) construct

<400>  11

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Ile Gln Leu Val Gln Ser Gly Pro Glu 
            20                  25                  30          


Leu Lys Lys Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Ile Phe Thr Asn Tyr Gly Met Asn Trp Val Lys Gln Ala Pro Gly 
    50                  55                  60                  


Lys Ser Phe Lys Trp Met Gly Trp Ile Asn Thr Tyr Thr Gly Glu Ser 
65                  70                  75                  80  


Thr Tyr Ser Ala Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr 
                85                  90                  95      


Ser Ala Ser Thr Ala Tyr Leu His Ile Asn Asp Leu Lys Asn Glu Asp 
            100                 105                 110         


Thr Ala Thr Tyr Phe Cys Ala Arg Ser Gly Gly Tyr Asp Pro Met Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr 
145                 150                 155                 160 


Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile 
                165                 170                 175     


Ser Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr Gly Asn Thr Phe Met 
            180                 185                 190         


His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
        195                 200                 205             


Arg Ala Ser Asn Leu Glu Ser Gly Ile Pro Ala Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Arg Thr Asp Phe Thr Leu Thr Ile Asn Pro Val Glu Ala Asp 
225                 230                 235                 240 


Asp Val Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Glu Asp Pro Pro Thr 
                245                 250                 255     


Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Glu Ser Lys Tyr Gly Pro 
            260                 265                 270         


Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val 
        275                 280                 285             


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
    290                 295                 300                 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu 
305                 310                 315                 320 


Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
                325                 330                 335     


Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser Thr Tyr Arg Val Val Ser 
            340                 345                 350         


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
        355                 360                 365             


Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile 
    370                 375                 380                 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
385                 390                 395                 400 


Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
                405                 410                 415     


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
            420                 425                 430         


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
        435                 440                 445             


Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg 
    450                 455                 460                 


Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
465                 470                 475                 480 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met 
                485                 490                 495     


Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
            500                 505                 510         


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
        515                 520                 525             


Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
    530                 535                 540                 


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
545                 550                 555                 560 


Ala Tyr Arg Ser Gly Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp 
                565                 570                 575     


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
            580                 585                 590         


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
        595                 600                 605             


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
    610                 615                 620                 


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
625                 630                 635                 640 


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
                645                 650                 655     


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
            660                 665                 670         


Met Gln Ala Leu Pro Pro Arg 
        675                 


<210>  12
<211>  672
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of the 26292CAR(S228P+L235E+N297Q) construct

<400>  12

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Gln Gln Pro Gly Ala Glu 
            20                  25                  30          


Leu Val Arg Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Ser Tyr Trp Met Asn Trp Val Lys Gln Arg Pro Asp 
    50                  55                  60                  


Gln Gly Leu Glu Trp Ile Gly Arg Ile Asp Pro Tyr Asp Ser Glu Thr 
65                  70                  75                  80  


His Tyr Asn Gln Lys Phe Lys Asp Lys Ala Ile Leu Thr Val Asp Lys 
                85                  90                  95      


Ser Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp 
            100                 105                 110         


Ser Ala Val Tyr Tyr Cys Ala Arg Gly Asn Trp Asp Asp Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Gln Ile Thr Gln Ser Pro 
145                 150                 155                 160 


Ser Tyr Leu Ala Ala Ser Pro Gly Glu Thr Ile Thr Ile Asn Cys Arg 
                165                 170                 175     


Ala Ser Lys Ser Ile Ser Lys Asp Leu Ala Trp Tyr Gln Glu Lys Pro 
            180                 185                 190         


Gly Lys Thr Asn Lys Leu Leu Ile Tyr Ser Gly Ser Thr Leu Gln Ser 
        195                 200                 205             


Gly Ile Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
    210                 215                 220                 


Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala Met Tyr Tyr Cys 
225                 230                 235                 240 


Gln Gln His Asn Lys Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu 
                245                 250                 255     


Glu Ile Lys Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala 
            260                 265                 270         


Pro Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
        275                 280                 285             


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
    290                 295                 300                 


Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val 
305                 310                 315                 320 


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
                325                 330                 335     


Phe Gln Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
            340                 345                 350         


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly 
        355                 360                 365             


Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
    370                 375                 380                 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr 
385                 390                 395                 400 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
                405                 410                 415     


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
            420                 425                 430         


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
        435                 440                 445             


Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe 
    450                 455                 460                 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
465                 470                 475                 480 


Ser Leu Ser Leu Ser Leu Gly Lys Met Phe Trp Val Leu Val Val Val 
                485                 490                 495     


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
            500                 505                 510         


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr 
        515                 520                 525             


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
    530                 535                 540                 


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly Gly 
545                 550                 555                 560 


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
                565                 570                 575     


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            580                 585                 590         


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        595                 600                 605             


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    610                 615                 620                 


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
625                 630                 635                 640 


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                645                 650                 655     


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            660                 665                 670         


<210>  13
<211>  229
<212>  PRT
<213>  Homo sapiens

<400>  13

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


