                               SEQUENCE LISTING

<110> OHIO STATE INNOVATION FOUNDATION
      RESEARCH INSTITUTE AT NATIONWIDE CHILDREN'S HOSPITAL
 
<120> METAPNEUMOVIRUS IMMUNOGENS AND RELATED MATERIALS AND METHODS

<130> 53-54826/OSIF-2013-030

<140>
<141>

<150> 61/790,707
<151> 2013-03-15

<160> 13    

<170> PatentIn version 3.5

<210> 1
<211> 1620
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 1
atgtcttgga aagtggtgat cattttttca ttgttaataa cacctcaaca cggtcttaaa       60

gagagctact tagaagagtc atgtagcact ataactgaag gatatctcag tgttctgagg      120

acaggttggt acaccaatgt ttttacactg gaggtaggcg atgtagagaa ccttacatgt      180

gccgatggac ccagcttaat aaaaacagaa ttagacctga ccaaaagtgc actaagagag      240

ctcagaacag tttctgctga tcaactggca agagaggagc aaattgaaaa tcccagacaa      300

tctagattcg ttctaggagc aatagcactc ggtgttgcaa ctgcagctgc agttacagca      360

ggtgttgcaa ttgccaaaac catccggctt gaaagtgaag taacagcaat taagaatgcc      420

ctcaaaaaga ccaatgaagc agtatctaca ttggggaatg gagttcgtgt gttggcaact      480

gcagtgagag agctgaaaga ttttgtgagc aagaatctaa cacgtgcaat caacaaaaac      540

aagtgcgaca ttgctgacct gaaaatggcc gttagcttca gtcaattcaa cagaaggttc      600

ctaaatgttg tgcggcaatt ttcagacaac gctggaataa caccagcaat atctttggac      660

ttaatgacag atgctgaact agccagagct gtttccaaca tgccaacatc tgcaggacaa      720

ataaaactga tgttggagaa ccgtgcaatg gtaagaagaa aagggttcgg attcctgata      780

ggagtttacg gaagctccgt aatttacatg gtgcaactgc caatctttgg ggttatagac      840

acgccttgct ggatagtaaa agcagcccct tcttgttcag gaaaaaaggg aaactatgct      900

tgcctcttaa gagaagacca aggatggtat tgtcaaaatg cagggtcaac tgtttactac      960

ccaaatgaaa aagactgtga aacaaaagga gaccatgtct tttgcgacac agcagcagga     1020

atcaatgttg ctgagcagtc aaaggagtgc aacataaaca tatctactac taattaccca     1080

tgcaaagtta gcacaggaag acatcctatc agtatggttg cactatctcc tcttggggct     1140

ttggttgctt gctacaaggg agtgagctgt tccattggca gcaacagagt agggatcatc     1200

aagcaactga acaaaggctg ctcttatata accaaccaag acgcagacac agtgacaata     1260

gacaacactg tataccagct aagcaaagtt gaaggcgaac agcatgttat aaaaggaagg     1320

ccagtgtcaa gcagctttga cccagtcaag tttcctgaag atcaattcaa tgttgcactt     1380

gaccaagttt tcgagagcat tgagaacagt caggccttgg tggatcaatc aaacagaatc     1440

ctaagcagtg cagagaaagg aaacactggc ttcatcattg taataattct aattgctgtc     1500

cttggctcta ccatgatcct agtgagtgtt tttatcataa taaagaaaac aaagaaaccc     1560

acaggagcac ctccagagct gagtggtgtc acaaacaatg gcttcatacc acataattag     1620


<210> 2
<211> 539
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 2
Met Ser Trp Lys Val Val Ile Ile Phe Ser Leu Leu Ile Thr Pro Gln 
1               5                   10                  15      


His Gly Leu Lys Glu Ser Tyr Leu Glu Glu Ser Cys Ser Thr Ile Thr 
            20                  25                  30          


Glu Gly Tyr Leu Ser Val Leu Arg Thr Gly Trp Tyr Thr Asn Val Phe 
        35                  40                  45              


Thr Leu Glu Val Gly Asp Val Glu Asn Leu Thr Cys Ala Asp Gly Pro 
    50                  55                  60                  


Ser Leu Ile Lys Thr Glu Leu Asp Leu Thr Lys Ser Ala Leu Arg Glu 
65                  70                  75                  80  


Leu Arg Thr Val Ser Ala Asp Gln Leu Ala Arg Glu Glu Gln Ile Glu 
                85                  90                  95      


Asn Pro Arg Gln Ser Arg Phe Val Leu Gly Ala Ile Ala Leu Gly Val 
            100                 105                 110         


Ala Thr Ala Ala Ala Val Thr Ala Gly Val Ala Ile Ala Lys Thr Ile 
        115                 120                 125             


Arg Leu Glu Ser Glu Val Thr Ala Ile Lys Asn Ala Leu Lys Lys Thr 
    130                 135                 140                 


Asn Glu Ala Val Ser Thr Leu Gly Asn Gly Val Arg Val Leu Ala Thr 
145                 150                 155                 160 


Ala Val Arg Glu Leu Lys Asp Phe Val Ser Lys Asn Leu Thr Arg Ala 
                165                 170                 175     


Ile Asn Lys Asn Lys Cys Asp Ile Ala Asp Leu Lys Met Ala Val Ser 
            180                 185                 190         


Phe Ser Gln Phe Asn Arg Arg Phe Leu Asn Val Val Arg Gln Phe Ser 
        195                 200                 205             


Asp Asn Ala Gly Ile Thr Pro Ala Ile Ser Leu Asp Leu Met Thr Asp 
    210                 215                 220                 


Ala Glu Leu Ala Arg Ala Val Ser Asn Met Pro Thr Ser Ala Gly Gln 
225                 230                 235                 240 


Ile Lys Leu Met Leu Glu Asn Arg Ala Met Val Arg Arg Lys Gly Phe 
                245                 250                 255     


Gly Phe Leu Ile Gly Val Tyr Gly Ser Ser Val Ile Tyr Met Val Gln 
            260                 265                 270         


Leu Pro Ile Phe Gly Val Ile Asp Thr Pro Cys Trp Ile Val Lys Ala 
        275                 280                 285             


Ala Pro Ser Cys Ser Gly Lys Lys Gly Asn Tyr Ala Cys Leu Leu Arg 
    290                 295                 300                 


Glu Asp Gln Gly Trp Tyr Cys Gln Asn Ala Gly Ser Thr Val Tyr Tyr 
305                 310                 315                 320 


Pro Asn Glu Lys Asp Cys Glu Thr Lys Gly Asp His Val Phe Cys Asp 
                325                 330                 335     


Thr Ala Ala Gly Ile Asn Val Ala Glu Gln Ser Lys Glu Cys Asn Ile 
            340                 345                 350         


Asn Ile Ser Thr Thr Asn Tyr Pro Cys Lys Val Ser Thr Gly Arg His 
        355                 360                 365             


Pro Ile Ser Met Val Ala Leu Ser Pro Leu Gly Ala Leu Val Ala Cys 
    370                 375                 380                 


Tyr Lys Gly Val Ser Cys Ser Ile Gly Ser Asn Arg Val Gly Ile Ile 
385                 390                 395                 400 


Lys Gln Leu Asn Lys Gly Cys Ser Tyr Ile Thr Asn Gln Asp Ala Asp 
                405                 410                 415     


Thr Val Thr Ile Asp Asn Thr Val Tyr Gln Leu Ser Lys Val Glu Gly 
            420                 425                 430         


Glu Gln His Val Ile Lys Gly Arg Pro Val Ser Ser Ser Phe Asp Pro 
        435                 440                 445             


Val Lys Phe Pro Glu Asp Gln Phe Asn Val Ala Leu Asp Gln Val Phe 
    450                 455                 460                 


Glu Ser Ile Glu Asn Ser Gln Ala Leu Val Asp Gln Ser Asn Arg Ile 
465                 470                 475                 480 


Leu Ser Ser Ala Glu Lys Gly Asn Thr Gly Phe Ile Ile Val Ile Ile 
                485                 490                 495     


Leu Ile Ala Val Leu Gly Ser Thr Met Ile Leu Val Ser Val Phe Ile 
            500                 505                 510         


Ile Ile Lys Lys Thr Lys Lys Pro Thr Gly Ala Pro Pro Glu Leu Ser 
        515                 520                 525             


Gly Val Thr Asn Asn Gly Phe Ile Pro His Asn 
    530                 535                 


<210> 3
<211> 1620
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 3
atgtcttgga aagtggtgat cattttttca ttgttaataa cacctcaaca cggtcttaaa       60

gagagctact tagaagagtc atgtagcact ataactgaag gatatctcag tgttctgagg      120

acaggttggt acaccaatgt ttttacactg gaggtaggcg atgtagagaa ccttacatgt      180

gccgatggac ccagcttaat aaaaacagaa ttagacctga ccaaaagtgc actaagagag      240

ctcagaacag tttctgctga tcaactggca agagaggagc aaattgaaaa tcccagacaa      300

tctagattcg ttctaggagc aatagcactc ggtgttgcaa ctgcagctgc agttacagca      360

ggtgttgcaa ttgccaaaac catccggctt gaaagtgaag taacagcaat taagaatgcc      420

ctcaaaaaga ccaatgaagc agtatctaca ttggggaatg gagttcgtgt gttggcaact      480

gcagtgagag agctgaaaga ttttgtgagc aagaatctaa cacgtgcaat caacaaaaac      540

aagtgcgaca ttgctgacct gaaaatggcc gttagcttca gtcaattcaa cagaaggttc      600

ctaaatgttg tgcggcaatt ttcagacaac gctggaataa caccagcaat atctttggac      660

ttaatgacag atgctgaact agccagagct gtttccaaca tgccaacatc tgcaggacaa      720

ataaaactga tgttggagaa ccgtgcaatg gtaagaagaa aagggttcgg attcctgata      780

ggagtttacg gaagctccgt aatttacatg gtgcaactgc caatctttgg ggttatagac      840

acgccttgct ggatagtaaa agcagcccct tcttgttcag gaaaaaaggg aaactatgct      900

tgcctcttaa gagaagacca aggatggtat tgtcaaaatg cagggtcaac tgtttactac      960

ccaaatgaaa aagactgtga aacaagagga gcccatgtct tttgcgacac agcagcagga     1020

atcaatgttg ctgagcagtc aaaggagtgc aacataaaca tatctactac taattaccca     1080

tgcaaagtta gcacaggaag acatcctatc agtatggttg cactatctcc tcttggggct     1140

ttggttgctt gctacaaggg agtgagctgt tccattggca gcaacagagt agggatcatc     1200

aagcaactga acaaaggctg ctcttatata accaaccaag acgcagacac agtgacaata     1260

gacaacactg tataccagct aagcaaagtt gaaggcgaac agcatgttat aaaaggaagg     1320

ccagtgtcaa gcagctttga cccagtcaag tttcctgaag atcaattcaa tgttgcactt     1380

gaccaagttt tcgagagcat tgagaacagt caggccttgg tggatcaatc aaacagaatc     1440

ctaagcagtg cagagaaagg aaacactggc ttcatcattg taataattct aattgctgtc     1500

cttggctcta ccatgatcct agtgagtgtt tttatcataa taaagaaaac aaagaaaccc     1560

acaggagcac ctccagagct gagtggtgtc acaaacaatg gcttcatacc acataattag     1620


<210> 4
<211> 539
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Met Ser Trp Lys Val Val Ile Ile Phe Ser Leu Leu Ile Thr Pro Gln 
1               5                   10                  15      


His Gly Leu Lys Glu Ser Tyr Leu Glu Glu Ser Cys Ser Thr Ile Thr 
            20                  25                  30          


Glu Gly Tyr Leu Ser Val Leu Arg Thr Gly Trp Tyr Thr Asn Val Phe 
        35                  40                  45              


Thr Leu Glu Val Gly Asp Val Glu Asn Leu Thr Cys Ala Asp Gly Pro 
    50                  55                  60                  


Ser Leu Ile Lys Thr Glu Leu Asp Leu Thr Lys Ser Ala Leu Arg Glu 
65                  70                  75                  80  


Leu Arg Thr Val Ser Ala Asp Gln Leu Ala Arg Glu Glu Gln Ile Glu 
                85                  90                  95      


Asn Pro Arg Gln Ser Arg Phe Val Leu Gly Ala Ile Ala Leu Gly Val 
            100                 105                 110         


Ala Thr Ala Ala Ala Val Thr Ala Gly Val Ala Ile Ala Lys Thr Ile 
        115                 120                 125             


Arg Leu Glu Ser Glu Val Thr Ala Ile Lys Asn Ala Leu Lys Lys Thr 
    130                 135                 140                 


Asn Glu Ala Val Ser Thr Leu Gly Asn Gly Val Arg Val Leu Ala Thr 
145                 150                 155                 160 


Ala Val Arg Glu Leu Lys Asp Phe Val Ser Lys Asn Leu Thr Arg Ala 
                165                 170                 175     


Ile Asn Lys Asn Lys Cys Asp Ile Ala Asp Leu Lys Met Ala Val Ser 
            180                 185                 190         


Phe Ser Gln Phe Asn Arg Arg Phe Leu Asn Val Val Arg Gln Phe Ser 
        195                 200                 205             


Asp Asn Ala Gly Ile Thr Pro Ala Ile Ser Leu Asp Leu Met Thr Asp 
    210                 215                 220                 


Ala Glu Leu Ala Arg Ala Val Ser Asn Met Pro Thr Ser Ala Gly Gln 
225                 230                 235                 240 


Ile Lys Leu Met Leu Glu Asn Arg Ala Met Val Arg Arg Lys Gly Phe 
                245                 250                 255     


Gly Phe Leu Ile Gly Val Tyr Gly Ser Ser Val Ile Tyr Met Val Gln 
            260                 265                 270         


Leu Pro Ile Phe Gly Val Ile Asp Thr Pro Cys Trp Ile Val Lys Ala 
        275                 280                 285             


Ala Pro Ser Cys Ser Gly Lys Lys Gly Asn Tyr Ala Cys Leu Leu Arg 
    290                 295                 300                 


Glu Asp Gln Gly Trp Tyr Cys Gln Asn Ala Gly Ser Thr Val Tyr Tyr 
305                 310                 315                 320 


Pro Asn Glu Lys Asp Cys Glu Thr Arg Gly Ala His Val Phe Cys Asp 
                325                 330                 335     


Thr Ala Ala Gly Ile Asn Val Ala Glu Gln Ser Lys Glu Cys Asn Ile 
            340                 345                 350         


Asn Ile Ser Thr Thr Asn Tyr Pro Cys Lys Val Ser Thr Gly Arg His 
        355                 360                 365             


Pro Ile Ser Met Val Ala Leu Ser Pro Leu Gly Ala Leu Val Ala Cys 
    370                 375                 380                 


Tyr Lys Gly Val Ser Cys Ser Ile Gly Ser Asn Arg Val Gly Ile Ile 
385                 390                 395                 400 


Lys Gln Leu Asn Lys Gly Cys Ser Tyr Ile Thr Asn Gln Asp Ala Asp 
                405                 410                 415     


Thr Val Thr Ile Asp Asn Thr Val Tyr Gln Leu Ser Lys Val Glu Gly 
            420                 425                 430         


Glu Gln His Val Ile Lys Gly Arg Pro Val Ser Ser Ser Phe Asp Pro 
        435                 440                 445             


Val Lys Phe Pro Glu Asp Gln Phe Asn Val Ala Leu Asp Gln Val Phe 
    450                 455                 460                 


Glu Ser Ile Glu Asn Ser Gln Ala Leu Val Asp Gln Ser Asn Arg Ile 
465                 470                 475                 480 


Leu Ser Ser Ala Glu Lys Gly Asn Thr Gly Phe Ile Ile Val Ile Ile 
                485                 490                 495     


Leu Ile Ala Val Leu Gly Ser Thr Met Ile Leu Val Ser Val Phe Ile 
            500                 505                 510         


Ile Ile Lys Lys Thr Lys Lys Pro Thr Gly Ala Pro Pro Glu Leu Ser 
        515                 520                 525             


Gly Val Thr Asn Asn Gly Phe Ile Pro His Asn 
    530                 535                 


<210> 5
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 5
His His His His His His 
1               5        


<210> 6
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 6
cggaattcgc caccatgtct tggaaagtgg tgatc                                  35


<210> 7
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 7
ataagaatgc ggccgcttag tgatggtgat ggtgatggcc agtgtttcct ttctctgc         58


<210> 8
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 8
cggaattcat gtcttggaaa gtggtgatc                                         29


<210> 9
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 9
ccgctcgagc taattatgtg gtatgaagc                                         29


<210> 10
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 10
ttataataca cgtctgcgcc c                                                 21


<210> 11
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 11
caccgccggg aaaatca                                                      17


<210> 12
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 12
gccttcagtt gggaaatttg g                                                 21


<210> 13
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      probe

<400> 13
atttgcagca gtccc                                                        15


