                         SEQUENCE LISTING

<110>  NBE Therapeutics
 
<120>  Method of producing an immunoligand/payload conjugate

<130>  ND40306

<160>  58    

<170>  PatentIn version 3.5

<210>  1
<211>  417
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH coding region of humanized anti-human CD19 antibody hBU12

<400>  1
atgggatgga gctggatctt tcttttcctc ctgtcaggaa ctgcaggtgt ccattgtcag       60

gttcagctgc aagagtctgg ccctgggttg gttaagccct cccagaccct cagtctgact      120

tgtactgtgt ctgggggttc aatcagcact tctggtatgg gtgtaggctg gattaggcag      180

cacccaggga agggtctgga gtggattgga cacatttggt gggatgatga caagagatat      240

aacccagccc tgaagagcag agtgacaatc tctgtggata cctccaagaa ccagtttagc      300

ctcaagctgt ccagtgtgac agctgcagat actgctgtct actactgtgc tagaatggaa      360

ctttggtcct actattttga ctactggggc caaggcaccc ttgtcacagt ctcctca         417


<210>  2
<211>  139
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of VH region of humanized anti-human CD19 
       antibody hBU12

<400>  2

Met Gly Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Thr Ala Gly 
1               5                   10                  15      


Val His Cys Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys 
            20                  25                  30          


Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile 
        35                  40                  45              


Ser Thr Ser Gly Met Gly Val Gly Trp Ile Arg Gln His Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly His Ile Trp Trp Asp Asp Asp Lys Arg Tyr 
65                  70                  75                  80  


Asn Pro Ala Leu Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys 
                85                  90                  95      


Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Met Glu Leu Trp Ser Tyr Tyr Phe Asp Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
    130                 135                 


<210>  3
<211>  375
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL coding region of humanized anti-human CD19 antibody hBU12

<400>  3
atgaagttgc ctgttaggct gttggtgctg atgttctgga ttcctgcttc cagcagtgaa       60

attgttctca cccagtctcc agcaaccctg tctctctctc caggggaaag ggctaccctg      120

agctgcagtg ccagctcaag tgtaagttac atgcactggt accagcagaa gccagggcag      180

gctcccagac tcctgattta tgacacatcc aaactggctt ctggtattcc agcaaggttc      240

agtggcagtg ggtctggaac agattttaca ctcacaatca gcagcctgga gccagaggat      300

gttgctgtct attactgttt tcaggggagt gtatacccat tcacttttgg ccaagggaca      360

aagttggaaa tcaaa                                                       375


<210>  4
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of VL region of humanized anti-human CD19 
       antibody hBU12

<400>  4

Met Lys Leu Pro Val Arg Leu Leu Val Leu Met Phe Trp Ile Pro Ala 
1               5                   10                  15      


Ser Ser Ser Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu 
            20                  25                  30          


Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Val 
        35                  40                  45              


Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu 
    50                  55                  60                  


Leu Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Ile Pro Ala Arg Phe 
65                  70                  75                  80  


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
                85                  90                  95      


Glu Pro Glu Asp Val Ala Val Tyr Tyr Cys Phe Gln Gly Ser Val Tyr 
            100                 105                 110         


Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125 


<210>  5
<211>  1083
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human IgG1 heavy chain constant coding region with in-frame 3' 
       extension encoding an LPETG sortase tag, an 6xHis tag and a 
       strepII tag

<400>  5
agcaccaagg gcccatctgt cttccccctg gcaccctcct ccaagagcac ctctgggggc       60

acagctgccc tgggctgcct ggtcaaggac tacttccctg aacctgtgac agtgtcctgg      120

aactcaggcg ccctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga      180

ctctactccc tcagcagcgt ggtgaccgtg ccctccagca gcttgggcac ccagacctac      240

atctgcaacg tgaatcacaa gcccagcaac accaaggtgg acaagaaagt tgagcccaaa      300

tcttgtgaca aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg      360

tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag      420

gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac      480

gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc      540

acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag      600

tacaagtgca aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa      660

gccaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggatgagctg      720

accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc      780

gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg      840

gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag      900

caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacacag      960

aagagcctct ccctgtctcc gggtaaactg cccgagaccg gccaccacca ccaccaccac     1020

ggcgagcaga agctgatcag cgaggaggac ctgggctgga gccaccccca gttcgagaag     1080

tag                                                                   1083


<210>  6
<211>  360
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of human IgG1 heavy chain constant region 
       with LPETG sortase tag, an 6xHis tag and a strepII tag

<400>  6

Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
1               5                   10                  15      


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
            20                  25                  30          


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
        35                  40                  45              


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
    50                  55                  60                  


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
65                  70                  75                  80  


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
                85                  90                  95      


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
            100                 105                 110         


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
        115                 120                 125             


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
    130                 135                 140                 


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
145                 150                 155                 160 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
                165                 170                 175     


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
            180                 185                 190         


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
        195                 200                 205             


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
    210                 215                 220                 


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
225                 230                 235                 240 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
                245                 250                 255     


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
            260                 265                 270         


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
        275                 280                 285             


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
    290                 295                 300                 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Leu Ser Leu Ser Pro Gly Lys Leu Pro Glu Thr Gly His His 
                325                 330                 335     


His His His His Gly Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Gly 
            340                 345                 350         


Trp Ser His Pro Gln Phe Glu Lys 
        355                 360 


<210>  7
<211>  414
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human IgG1 kappa light chain constant coding region with in-frame
       3' extension encoding an LPETG sortase tag, an 6xHis tag and a 
       strepII tag.

<400>  7
acggtggctg caccatctgt cttcatcttc ccgccatctg atgagcagtt gaaatctgga       60

actgcctctg ttgtgtgcct gctgaataac ttctatccca gagaggccaa agtacagtgg      120

aaggtggata acgccctcca atcgggtaac tcccaggaga gtgtcacaga gcaggacagc      180

aaggacagca cctacagcct cagcagcacc ctgacgctga gcaaagcaga ctacgagaaa      240

cacaaagtct acgcctgcga agtcacccat cagggcctga gctcgcccgt cacaaagagc      300

ttcaacaggg gagagtgtct gcccgagacc ggccaccacc accaccacca cggcgagcag      360

aagctgatca gcgaggagga cctgggctgg agccaccccc agttcgagaa gtag            414


<210>  8
<211>  137
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of human IgG1 kappa light chain constant 
       region with LPETG sortase tag, an 6xHis tag and a strepII tag.

<400>  8

Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
1               5                   10                  15      


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
            20                  25                  30          


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
        35                  40                  45              


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
    50                  55                  60                  


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
65                  70                  75                  80  


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
                85                  90                  95      


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Leu Pro Glu Thr Gly His 
            100                 105                 110         


His His His His His Gly Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
        115                 120                 125             


Gly Trp Ser His Pro Gln Phe Glu Lys 
    130                 135         


<210>  9
<211>  1503
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Complete human IgG1 VH-CH heavy chain coding region for hBU12 
       with C-terminal LPETG sortase tag, 6xHis tag and a strepII tag

<400>  9
atgggatgga gctggatctt tcttttcctc ctgtcaggaa ctgcaggtgt ccattgtcag       60

gttcagctgc aagagtctgg ccctgggttg gttaagccct cccagaccct cagtctgact      120

tgtactgtgt ctgggggttc aatcagcact tctggtatgg gtgtaggctg gattaggcag      180

cacccaggga agggtctgga gtggattgga cacatttggt gggatgatga caagagatat      240

aacccagccc tgaagagcag agtgacaatc tctgtggata cctccaagaa ccagtttagc      300

ctcaagctgt ccagtgtgac agctgcagat actgctgtct actactgtgc tagaatggaa      360

ctttggtcct actattttga ctactggggc caaggcaccc ttgtcacagt ctcctcagct      420

agcaccaagg gcccatctgt cttccccctg gcaccctcct ccaagagcac ctctgggggc      480

acagctgccc tgggctgcct ggtcaaggac tacttccctg aacctgtgac agtgtcctgg      540

aactcaggcg ccctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga      600

ctctactccc tcagcagcgt ggtgaccgtg ccctccagca gcttgggcac ccagacctac      660

atctgcaacg tgaatcacaa gcccagcaac accaaggtgg acaagaaagt tgagcccaaa      720

tcttgtgaca aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg      780

tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag      840

gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac      900

gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc      960

acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag     1020

tacaagtgca aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa     1080

gccaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggatgagctg     1140

accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc     1200

gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg     1260

gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag     1320

caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacacag     1380

aagagcctct ccctgtctcc gggtaaactg cccgagaccg gccaccacca ccaccaccac     1440

ggcgagcaga agctgatcag cgaggaggac ctgggctgga gccaccccca gttcgagaag     1500

tag                                                                   1503


<210>  10
<211>  500
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of complete human IgG1 VH-CH heavy chain 
       region of hBU12 with C-terminal LPETG sortase tag, 6xHis tag and 
       a strepII tag

<400>  10

Met Gly Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Thr Ala Gly 
1               5                   10                  15      


Val His Cys Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys 
            20                  25                  30          


Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile 
        35                  40                  45              


Ser Thr Ser Gly Met Gly Val Gly Trp Ile Arg Gln His Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly His Ile Trp Trp Asp Asp Asp Lys Arg Tyr 
65                  70                  75                  80  


Asn Pro Ala Leu Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys 
                85                  90                  95      


Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Met Glu Leu Trp Ser Tyr Tyr Phe Asp Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
    130                 135                 140                 


Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
145                 150                 155                 160 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
                165                 170                 175     


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
            180                 185                 190         


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
        195                 200                 205             


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
    210                 215                 220                 


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys 
225                 230                 235                 240 


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
                245                 250                 255     


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            260                 265                 270         


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        275                 280                 285             


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
    290                 295                 300                 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
305                 310                 315                 320 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                325                 330                 335     


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
            340                 345                 350         


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        355                 360                 365             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln 
    370                 375                 380                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
385                 390                 395                 400 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                405                 410                 415     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            420                 425                 430         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        435                 440                 445             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    450                 455                 460                 


Leu Ser Pro Gly Lys Leu Pro Glu Thr Gly His His His His His His 
465                 470                 475                 480 


Gly Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Gly Trp Ser His Pro 
                485                 490                 495     


Gln Phe Glu Lys 
            500 


<210>  11
<211>  792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Complete human IgG1 VL-CL kappa chain coding region for hBU12 
       with C-terminal LPETG sortase tag, 6xHis tag and a strepII tag

<400>  11
atgaagttgc ctgttaggct gttggtgctg atgttctgga ttcctgcttc cagcagtgaa       60

attgttctca cccagtctcc agcaaccctg tctctctctc caggggaaag ggctaccctg      120

agctgcagtg ccagctcaag tgtaagttac atgcactggt accagcagaa gccagggcag      180

gctcccagac tcctgattta tgacacatcc aaactggctt ctggtattcc agcaaggttc      240

agtggcagtg ggtctggaac agattttaca ctcacaatca gcagcctgga gccagaggat      300

gttgctgtct attactgttt tcaggggagt gtatacccat tcacttttgg ccaagggaca      360

aagttggaaa tcaaaagaac tgtggctgca ccatctgtct tcatcttccc gccatctgat      420

gagcagttga aatctggaac tgcctctgtt gtgtgcctgc tgaataactt ctatcccaga      480

gaggccaaag tacagtggaa ggtggataac gccctccaat cgggtaactc ccaggagagt      540

gtcacagagc aggacagcaa ggacagcacc tacagcctca gcagcaccct gacgctgagc      600

aaagcagact acgagaaaca caaagtctac gcctgcgaag tcacccatca gggcctgagc      660

tcgcccgtca caaagagctt caacagggga gagtgtctgc ccgagaccgg ccaccaccac      720

caccaccacg gcgagcagaa gctgatcagc gaggaggacc tgggctggag ccacccccag      780

ttcgagaagt ag                                                          792


<210>  12
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of complete human IgG1 VL-CL kappa chain 
       region of hBU12 with C-terminal LPETG sortase tag, 6xHis tag and 
       a strepII tag

<400>  12

Met Lys Leu Pro Val Arg Leu Leu Val Leu Met Phe Trp Ile Pro Ala 
1               5                   10                  15      


Ser Ser Ser Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu 
            20                  25                  30          


Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Val 
        35                  40                  45              


Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu 
    50                  55                  60                  


Leu Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Ile Pro Ala Arg Phe 
65                  70                  75                  80  


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
                85                  90                  95      


Glu Pro Glu Asp Val Ala Val Tyr Tyr Cys Phe Gln Gly Ser Val Tyr 
            100                 105                 110         


Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val 
        115                 120                 125             


Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys 
    130                 135                 140                 


Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg 
145                 150                 155                 160 


Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn 
                165                 170                 175     


Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser 
            180                 185                 190         


Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
        195                 200                 205             


Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr 
    210                 215                 220                 


Lys Ser Phe Asn Arg Gly Glu Cys Leu Pro Glu Thr Gly His His His 
225                 230                 235                 240 


His His His Gly Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Gly Trp 
                245                 250                 255     


Ser His Pro Gln Phe Glu Lys 
            260             


<210>  13
<211>  7045
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  coding region of human IgG1 VH-CH heavy chain for hBU12 with 
       C-terminal LPETG sortase tag, 6xHis tag and a strepII tag and 
       HindIII and NotI cloning sites

<400>  13
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttccat gggatggagc tggatctttc ttttcctcct gtcaggaact      960

gcaggtgtcc attgtcaggt tcagctgcaa gagtctggcc ctgggttggt taagccctcc     1020

cagaccctca gtctgacttg tactgtgtct gggggttcaa tcagcacttc tggtatgggt     1080

gtaggctgga ttaggcagca cccagggaag ggtctggagt ggattggaca catttggtgg     1140

gatgatgaca agagatataa cccagccctg aagagcagag tgacaatctc tgtggatacc     1200

tccaagaacc agtttagcct caagctgtcc agtgtgacag ctgcagatac tgctgtctac     1260

tactgtgcta gaatggaact ttggtcctac tattttgact actggggcca aggcaccctt     1320

gtcacagtct cctcagctag caccaagggc ccatctgtct tccccctggc accctcctcc     1380

aagagcacct ctgggggcac agctgccctg ggctgcctgg tcaaggacta cttccctgaa     1440

cctgtgacag tgtcctggaa ctcaggcgcc ctgaccagcg gcgtgcacac cttcccggct     1500

gtcctacagt cctcaggact ctactccctc agcagcgtgg tgaccgtgcc ctccagcagc     1560

ttgggcaccc agacctacat ctgcaacgtg aatcacaagc ccagcaacac caaggtggac     1620

aagaaagttg agcccaaatc ttgtgacaaa actcacacat gcccaccgtg cccagcacct     1680

gaactcctgg ggggaccgtc agtcttcctc ttccccccaa aacccaagga caccctcatg     1740

atctcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga agaccctgag     1800

gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac aaagccgcgg     1860

gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac     1920

tggctgaatg gcaaggagta caagtgcaag gtctccaaca aagccctccc agcccccatc     1980

gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta caccctgccc     2040

ccatcccggg atgagctgac caagaaccag gtcagcctga cctgcctggt caaaggcttc     2100

tatcccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa caactacaag     2160

accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcaa gctcaccgtg     2220

gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg     2280

cacaaccact acacacagaa gagcctctcc ctgtctccgg gtaaactgcc cgagaccggc     2340

caccaccacc accaccacgg cgagcagaag ctgatcagcg aggaggacct gggctggagc     2400

cacccccagt tcgagaagta ggcggccgct cgagtctaga gggcccgttt aaacccgctg     2460

atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc     2520

ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc     2580

atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa     2640

gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc     2700

tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc     2760

attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct     2820

agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg     2880

tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga     2940

ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt     3000

ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg     3060

aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc     3120

ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg     3180

aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca     3240

aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg     3300

cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc     3360

gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat     3420

tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg     3480

aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat     3540

tttcggatct gatcagcacg tgatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa     3600

gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga     3660

atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg     3720

cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc     3780

gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg     3840

ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca     3900

gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt     3960

cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc     4020

gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc     4080

cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca     4140

cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc     4200

ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt     4260

cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca     4320

tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca     4380

actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg     4440

cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag     4500

cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc     4560

cagcactcgt ccgagggcaa aggaatagca cgtgctacga gatttcgatt ccaccgccgc     4620

cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca     4680

gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa     4740

tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca     4800

ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac     4860

ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc     4920

gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta     4980

atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa     5040

cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat     5100

tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg     5160

agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc     5220

aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt     5280

gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag     5340

tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc     5400

cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc     5460

ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt     5520

cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt     5580

atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc     5640

agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa     5700

gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa     5760

gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg     5820

tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga     5880

agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg     5940

gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg     6000

aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt     6060

aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact     6120

ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat     6180

gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg     6240

aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg     6300

ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat     6360

tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc     6420

ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt     6480

cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc     6540

agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga     6600

gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc     6660

gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa     6720

acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta     6780

acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg     6840

agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg     6900

aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat     6960

gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt     7020

tccccgaaaa gtgccacctg acgtc                                           7045


<210>  14
<211>  6334
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  coding region of human IgG1 VL-CL kappa light chain for hBU12 
       with C-terminal LPETG sortase tag, 6xHis tag and a strepII tag 
       and HindIII and NotI cloning sites

<400>  14
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttccat gaagttgcct gttaggctgt tggtgctgat gttctggatt      960

cctgcttcca gcagtgaaat tgttctcacc cagtctccag caaccctgtc tctctctcca     1020

ggggaaaggg ctaccctgag ctgcagtgcc agctcaagtg taagttacat gcactggtac     1080

cagcagaagc cagggcaggc tcccagactc ctgatttatg acacatccaa actggcttct     1140

ggtattccag caaggttcag tggcagtggg tctggaacag attttacact cacaatcagc     1200

agcctggagc cagaggatgt tgctgtctat tactgttttc aggggagtgt atacccattc     1260

acttttggcc aagggacaaa gttggaaatc aaaagaactg tggctgcacc atctgtcttc     1320

atcttcccgc catctgatga gcagttgaaa tctggaactg cctctgttgt gtgcctgctg     1380

aataacttct atcccagaga ggccaaagta cagtggaagg tggataacgc cctccaatcg     1440

ggtaactccc aggagagtgt cacagagcag gacagcaagg acagcaccta cagcctcagc     1500

agcaccctga cgctgagcaa agcagactac gagaaacaca aagtctacgc ctgcgaagtc     1560

acccatcagg gcctgagctc gcccgtcaca aagagcttca acaggggaga gtgtctgccc     1620

gagaccggcc accaccacca ccaccacggc gagcagaagc tgatcagcga ggaggacctg     1680

ggctggagcc acccccagtt cgagaagtag gcggccgctc gagtctagag ggcccgttta     1740

aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc     1800

ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga     1860

ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca     1920

ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc     1980

tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg     2040

tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc     2100

cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg     2160

ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg     2220

gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg     2280

atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt     2340

ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt     2400

ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta     2460

attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag     2520

aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc     2580

cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc     2640

cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg     2700

ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca     2760

gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg     2820

tatatccatt ttcggatctg atcagcacgt gatgaaaaag cctgaactca ccgcgacgtc     2880

tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga     2940

gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt     3000

aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc     3060

cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg     3120

catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc     3180

tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac     3240

gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt     3300

catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt     3360

cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga     3420

agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg     3480

cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc     3540

caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga     3600

gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg     3660

tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca     3720

gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc     3780

ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa     3840

ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtgctacgag atttcgattc     3900

caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat     3960

gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc     4020

agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt     4080

ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat     4140

accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa     4200

ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg     4260

gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca     4320

gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg     4380

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg     4440

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg     4500

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa     4560

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg     4620

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc     4680

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc     4740

ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc     4800

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg     4860

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc     4920

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga     4980

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc     5040

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac     5100

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     5160

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc     5220

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa     5280

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta     5340

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt     5400

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag     5460

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca     5520

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc     5580

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt     5640

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag     5700

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt     5760

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat     5820

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt     5880

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc     5940

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat     6000

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag     6060

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt     6120

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg     6180

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta     6240

ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc     6300

gcgcacattt ccccgaaaag tgccacctga cgtc                                 6334


<210>  15
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of the N-intein domain of Ssp GyrB 11 
       split-intein

<400>  15

Cys Phe Ser Gly Asp Thr Leu Val Ala Leu Thr Asp Gly Arg Ser Val 
1               5                   10                  15      


Ser Phe Glu Gln Leu Val Glu Glu Glu Lys Gln Gly Lys Gln Asn Phe 
            20                  25                  30          


Cys Tyr Thr Ile Arg His Asp Gly Ser Ile Gly Val Glu Lys Ile Ile 
        35                  40                  45              


Asn Ala Arg Lys Thr Lys Thr Asn Ala Lys Val Ile Lys Val Thr Leu 
    50                  55                  60                  


Asp Asn Gly Glu Ser Ile Ile Cys Thr Pro Asp His Lys Phe Met Leu 
65                  70                  75                  80  


Arg Asp Gly Ser Tyr Lys Cys Ala Met Asp Leu Thr Leu Asp Asp Ser 
                85                  90                  95      


Leu Met Pro Leu His Arg Lys Ile Ser Thr Thr Glu Asp Ser Gly His 
            100                 105                 110         


Met Glu Ala Val Leu Asn Tyr Asn His Arg Ile Val Asn Ile Glu Ala 
        115                 120                 125             


Val Ser Glu Thr Ile Asp Val Tyr Asp Ile Glu Val Pro His Thr His 
    130                 135                 140                 


Asn Phe Ala Leu Ala Ser 
145                 150 


<210>  16
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse translation of SEQ ID NO 15 with mammalian codon usage 
       results in the coding sequence for the N-intein domain of Ssp 
       GyrB 11 split-intein

<400>  16
tgcttcagcg gcgacaccct ggtggccctg accgacggca gaagcgtgag cttcgagcag       60

ctggtggagg aggagaagca gggcaagcag aacttctgct acaccatcag acacgacggc      120

agcatcggcg tggagaagat catcaacgcc agaaagacca agaccaacgc caaggtgatc      180

aaggtgaccc tggacaacgg cgagagcatc atctgcaccc ccgaccacaa gttcatgctg      240

agagacggca gctacaagtg cgccatggac ctgaccctgg acgacagcct gatgcccctg      300

cacagaaaga tcagcaccac cgaggacagc ggccacatgg aggccgtgct gaactacaac      360

cacagaatcg tgaacatcga ggccgtgagc gagaccatcg acgtgtacga catcgaggtg      420

ccccacaccc acaacttcgc cctggccagc                                       450


<210>  17
<211>  1905
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  complete IgG1 heavy chain coding region for anti-human CD19 
       antibody hBU12 with C-terminal extension, comprising the N-intein
       domain of Ssp GyrB 11 split-intein, followed by a 6xHis-tag and a
       strepII tag

<400>  17
atgaattttg gactgaggct gattttcctg gtgctgaccc tgaaaggcgt ccagtgtcag       60

gttcagctgc aagagtctgg ccctgggttg gttaagccct cccagaccct cagtctgact      120

tgtactgtgt ctgggggttc aatcagcact tctggtatgg gtgtaggctg gattaggcag      180

cacccaggga agggtctgga gtggattgga cacatttggt gggatgatga caagagatat      240

aacccagccc tgaagagcag agtgacaatc tctgtggata cctccaagaa ccagtttagc      300

ctcaagctgt ccagtgtgac agctgcagat actgctgtct actactgtgc tagaatggaa      360

ctttggtcct actattttga ctactggggc caaggcaccc ttgtcacagt ctcctcagct      420

agcaccaagg gcccatctgt cttccccctg gcaccctcct ccaagagcac ctctgggggc      480

acagctgccc tgggctgcct ggtcaaggac tacttccctg aacctgtgac agtgtcctgg      540

aactcaggcg ccctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga      600

ctctactccc tcagcagcgt ggtgaccgtg ccctccagca gcttgggcac ccagacctac      660

atctgcaacg tgaatcacaa gcccagcaac accaaggtgg acaagaaagt tgagcccaaa      720

tcttgtgaca aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg      780

tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag      840

gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac      900

gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc      960

acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag     1020

tacaagtgca aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa     1080

gccaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggatgagctg     1140

accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc     1200

gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg     1260

gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag     1320

caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacacag     1380

aagagcctct ccctgtctcc gggtaaatgc ttcagcggcg acaccctggt ggccctgacc     1440

gacggcagaa gcgtgagctt cgagcagctg gtggaggagg agaagcaggg caagcagaac     1500

ttctgctaca ccatcagaca cgacggcagc atcggcgtgg agaagatcat caacgccaga     1560

aagaccaaga ccaacgccaa ggtgatcaag gtgaccctgg acaacggcga gagcatcatc     1620

tgcacccccg accacaagtt catgctgaga gacggcagct acaagtgcgc catggacctg     1680

accctggacg acagcctgat gcccctgcac agaaagatca gcaccaccga ggacagcggc     1740

cacatggagg ccgtgctgaa ctacaaccac agaatcgtga acatcgaggc cgtgagcgag     1800

accatcgacg tgtacgacat cgaggtgccc cacacccaca acttcgccct ggccagccac     1860

catcaccatc accatggctg gagccacccc cagttcgaga agtag                     1905


<210>  18
<211>  634
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence translated from SEQ ID NO 17

<400>  18

Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys 
            20                  25                  30          


Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile 
        35                  40                  45              


Ser Thr Ser Gly Met Gly Val Gly Trp Ile Arg Gln His Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly His Ile Trp Trp Asp Asp Asp Lys Arg Tyr 
65                  70                  75                  80  


Asn Pro Ala Leu Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys 
                85                  90                  95      


Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Met Glu Leu Trp Ser Tyr Tyr Phe Asp Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
    130                 135                 140                 


Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
145                 150                 155                 160 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
                165                 170                 175     


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
            180                 185                 190         


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
        195                 200                 205             


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
    210                 215                 220                 


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys 
225                 230                 235                 240 


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
                245                 250                 255     


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            260                 265                 270         


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        275                 280                 285             


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
    290                 295                 300                 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
305                 310                 315                 320 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                325                 330                 335     


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
            340                 345                 350         


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        355                 360                 365             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln 
    370                 375                 380                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
385                 390                 395                 400 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                405                 410                 415     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            420                 425                 430         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        435                 440                 445             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    450                 455                 460                 


Leu Ser Pro Gly Lys Cys Phe Ser Gly Asp Thr Leu Val Ala Leu Thr 
465                 470                 475                 480 


Asp Gly Arg Ser Val Ser Phe Glu Gln Leu Val Glu Glu Glu Lys Gln 
                485                 490                 495     


Gly Lys Gln Asn Phe Cys Tyr Thr Ile Arg His Asp Gly Ser Ile Gly 
            500                 505                 510         


Val Glu Lys Ile Ile Asn Ala Arg Lys Thr Lys Thr Asn Ala Lys Val 
        515                 520                 525             


Ile Lys Val Thr Leu Asp Asn Gly Glu Ser Ile Ile Cys Thr Pro Asp 
    530                 535                 540                 


His Lys Phe Met Leu Arg Asp Gly Ser Tyr Lys Cys Ala Met Asp Leu 
545                 550                 555                 560 


Thr Leu Asp Asp Ser Leu Met Pro Leu His Arg Lys Ile Ser Thr Thr 
                565                 570                 575     


Glu Asp Ser Gly His Met Glu Ala Val Leu Asn Tyr Asn His Arg Ile 
            580                 585                 590         


Val Asn Ile Glu Ala Val Ser Glu Thr Ile Asp Val Tyr Asp Ile Glu 
        595                 600                 605             


Val Pro His Thr His Asn Phe Ala Leu Ala Ser His His His His His 
    610                 615                 620                 


His Gly Trp Ser His Pro Gln Phe Glu Lys 
625                 630                 


<210>  19
<211>  1206
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  complete IgG1 kappa light chain coding region for anti-human CD19
       antibody hBU12 with C-terminal extension, comprising the N-intein
       domain of Ssp GyrB 11 split-intein, followed by a 6xHis-tag and a
       strepII tag

<400>  19
atgaattttg gactgaggct gattttcctg gtgctgaccc tgaaaggcgt ccagtgtgac       60

attgtgctga cccaatctcc agcttctttg gctgtgtctc tagggcagag ggccaccatc      120

tcctgcaagg ccagccaaag tgttgatttt gatggtgata gttatatgaa ctggtaccaa      180

cagaaaccag gacagccacc caaagtcctc atctatgctg catccaatct agaatctggg      240

atcccagcca ggtttagtgg cagtgggtct gggacagact tcaccctcaa catccatcct      300

gtggaggagg aggatgctgc aacctattac tgtcagcaaa gtaatgagga tccgtggacg      360

ttcggtggag gcaccaagct ggaaatcaaa cgtacggtgg ctgcaccatc tgtcttcatc      420

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat      480

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt      540

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc      600

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc      660

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg cttcagcggc      720

gacaccctgg tggccctgac cgacggcaga agcgtgagct tcgagcagct ggtggaggag      780

gagaagcagg gcaagcagaa cttctgctac accatcagac acgacggcag catcggcgtg      840

gagaagatca tcaacgccag aaagaccaag accaacgcca aggtgatcaa ggtgaccctg      900

gacaacggcg agagcatcat ctgcaccccc gaccacaagt tcatgctgag agacggcagc      960

tacaagtgcg ccatggacct gaccctggac gacagcctga tgcccctgca cagaaagatc     1020

agcaccaccg aggacagcgg ccacatggag gccgtgctga actacaacca cagaatcgtg     1080

aacatcgagg ccgtgagcga gaccatcgac gtgtacgaca tcgaggtgcc ccacacccac     1140

aacttcgccc tggccagcca ccatcaccat caccatggct ggagccaccc ccagttcgag     1200

aagtag                                                                1206


<210>  20
<211>  401
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence translated from SEQ ID NO 19

<400>  20

Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val 
        35                  40                  45              


Asp Phe Asp Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Gln Pro Pro Lys Val Leu Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly 
65                  70                  75                  80  


Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Asn Ile His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Ser Asn Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu 
        115                 120                 125             


Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser 
    130                 135                 140                 


Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn 
145                 150                 155                 160 


Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala 
                165                 170                 175     


Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys 
            180                 185                 190         


Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp 
        195                 200                 205             


Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu 
    210                 215                 220                 


Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Phe Ser Gly 
225                 230                 235                 240 


Asp Thr Leu Val Ala Leu Thr Asp Gly Arg Ser Val Ser Phe Glu Gln 
                245                 250                 255     


Leu Val Glu Glu Glu Lys Gln Gly Lys Gln Asn Phe Cys Tyr Thr Ile 
            260                 265                 270         


Arg His Asp Gly Ser Ile Gly Val Glu Lys Ile Ile Asn Ala Arg Lys 
        275                 280                 285             


Thr Lys Thr Asn Ala Lys Val Ile Lys Val Thr Leu Asp Asn Gly Glu 
    290                 295                 300                 


Ser Ile Ile Cys Thr Pro Asp His Lys Phe Met Leu Arg Asp Gly Ser 
305                 310                 315                 320 


Tyr Lys Cys Ala Met Asp Leu Thr Leu Asp Asp Ser Leu Met Pro Leu 
                325                 330                 335     


His Arg Lys Ile Ser Thr Thr Glu Asp Ser Gly His Met Glu Ala Val 
            340                 345                 350         


Leu Asn Tyr Asn His Arg Ile Val Asn Ile Glu Ala Val Ser Glu Thr 
        355                 360                 365             


Ile Asp Val Tyr Asp Ile Glu Val Pro His Thr His Asn Phe Ala Leu 
    370                 375                 380                 


Ala Ser His His His His His His Gly Trp Ser His Pro Gln Phe Glu 
385                 390                 395                 400 


Lys 
    


<210>  21
<211>  7447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  coding region of human IgG1 VH-CH heavy chain for hBU12 with 
       C-terminal N-intein domain of Ssp GyrB S11 split intein, followed
       by 6xHis tag strepII tag and HindIII and NotI cloning sites

<400>  21
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttccat gaattttgga ctgaggctga ttttcctggt gctgaccctg      960

aaaggcgtcc agtgtcaggt tcagctgcaa gagtctggcc ctgggttggt taagccctcc     1020

cagaccctca gtctgacttg tactgtgtct gggggttcaa tcagcacttc tggtatgggt     1080

gtaggctgga ttaggcagca cccagggaag ggtctggagt ggattggaca catttggtgg     1140

gatgatgaca agagatataa cccagccctg aagagcagag tgacaatctc tgtggatacc     1200

tccaagaacc agtttagcct caagctgtcc agtgtgacag ctgcagatac tgctgtctac     1260

tactgtgcta gaatggaact ttggtcctac tattttgact actggggcca aggcaccctt     1320

gtcacagtct cctcagctag caccaagggc ccatctgtct tccccctggc accctcctcc     1380

aagagcacct ctgggggcac agctgccctg ggctgcctgg tcaaggacta cttccctgaa     1440

cctgtgacag tgtcctggaa ctcaggcgcc ctgaccagcg gcgtgcacac cttcccggct     1500

gtcctacagt cctcaggact ctactccctc agcagcgtgg tgaccgtgcc ctccagcagc     1560

ttgggcaccc agacctacat ctgcaacgtg aatcacaagc ccagcaacac caaggtggac     1620

aagaaagttg agcccaaatc ttgtgacaaa actcacacat gcccaccgtg cccagcacct     1680

gaactcctgg ggggaccgtc agtcttcctc ttccccccaa aacccaagga caccctcatg     1740

atctcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga agaccctgag     1800

gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac aaagccgcgg     1860

gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac     1920

tggctgaatg gcaaggagta caagtgcaag gtctccaaca aagccctccc agcccccatc     1980

gagaaaacca tctccaaagc caaagggcag ccccgagaac cacaggtgta caccctgccc     2040

ccatcccggg atgagctgac caagaaccag gtcagcctga cctgcctggt caaaggcttc     2100

tatcccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa caactacaag     2160

accacgcctc ccgtgctgga ctccgacggc tccttcttcc tctacagcaa gctcaccgtg     2220

gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg     2280

cacaaccact acacacagaa gagcctctcc ctgtctccgg gtaaatgctt cagcggcgac     2340

accctggtgg ccctgaccga cggcagaagc gtgagcttcg agcagctggt ggaggaggag     2400

aagcagggca agcagaactt ctgctacacc atcagacacg acggcagcat cggcgtggag     2460

aagatcatca acgccagaaa gaccaagacc aacgccaagg tgatcaaggt gaccctggac     2520

aacggcgaga gcatcatctg cacccccgac cacaagttca tgctgagaga cggcagctac     2580

aagtgcgcca tggacctgac cctggacgac agcctgatgc ccctgcacag aaagatcagc     2640

accaccgagg acagcggcca catggaggcc gtgctgaact acaaccacag aatcgtgaac     2700

atcgaggccg tgagcgagac catcgacgtg tacgacatcg aggtgcccca cacccacaac     2760

ttcgccctgg ccagccacca tcaccatcac catggctgga gccaccccca gttcgagaag     2820

taggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc     2880

ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg     2940

tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag     3000

gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga     3060

caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag     3120

ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt     3180

ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc     3240

tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg     3300

catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta     3360

gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt     3420

ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat     3480

ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa     3540

tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg     3600

tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta     3660

gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat     3720

gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac     3780

tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga     3840

ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg     3900

cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca     3960

cgtgatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt     4020

cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt     4080

cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa     4140

agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga     4200

cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac     4260

gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat     4320

ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca     4380

aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt     4440

gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga     4500

tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt     4560

cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga     4620

ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt     4680

ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc     4740

gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt     4800

tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc     4860

cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga     4920

tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc     4980

aaaggaatag cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg     5040

cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct     5100

ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa     5160

tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc     5220

caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc     5280

gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa     5340

catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac     5400

attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca     5460

ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc     5520

ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc     5580

aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc     5640

aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag     5700

gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc     5760

gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt     5820

tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct     5880

ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg     5940

ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct     6000

tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat     6060

tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg     6120

ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa     6180

aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt     6240

ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc     6300

tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt     6360

atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta     6420

aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat     6480

ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac     6540

tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg     6600

ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag     6660

tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt     6720

aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt     6780

gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt     6840

tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt     6900

cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct     6960

tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt     7020

ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac     7080

cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa     7140

actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa     7200

ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca     7260

aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct     7320

ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga     7380

atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc     7440

tgacgtc                                                               7447


<210>  22
<211>  6748
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  coding region of human IgG1 VL-CL kappa light chain for hBU12 
       with C-terminal Ssp GyrB S11 N-intein domain, 6xHis tag and a 
       strepII tag, and HindIII and NotI cloning sites

<400>  22
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttccat gaattttgga ctgaggctga ttttcctggt gctgaccctg      960

aaaggcgtcc agtgtgacat tgtgctgacc caatctccag cttctttggc tgtgtctcta     1020

gggcagaggg ccaccatctc ctgcaaggcc agccaaagtg ttgattttga tggtgatagt     1080

tatatgaact ggtaccaaca gaaaccagga cagccaccca aagtcctcat ctatgctgca     1140

tccaatctag aatctgggat cccagccagg tttagtggca gtgggtctgg gacagacttc     1200

accctcaaca tccatcctgt ggaggaggag gatgctgcaa cctattactg tcagcaaagt     1260

aatgaggatc cgtggacgtt cggtggaggc accaagctgg aaatcaaacg tacggtggct     1320

gcaccatctg tcttcatctt cccgccatct gatgagcagt tgaaatctgg aactgcctct     1380

gttgtgtgcc tgctgaataa cttctatccc agagaggcca aagtacagtg gaaggtggat     1440

aacgccctcc aatcgggtaa ctcccaggag agtgtcacag agcaggacag caaggacagc     1500

acctacagcc tcagcagcac cctgacgctg agcaaagcag actacgagaa acacaaagtc     1560

tacgcctgcg aagtcaccca tcagggcctg agctcgcccg tcacaaagag cttcaacagg     1620

ggagagtgct tcagcggcga caccctggtg gccctgaccg acggcagaag cgtgagcttc     1680

gagcagctgg tggaggagga gaagcagggc aagcagaact tctgctacac catcagacac     1740

gacggcagca tcggcgtgga gaagatcatc aacgccagaa agaccaagac caacgccaag     1800

gtgatcaagg tgaccctgga caacggcgag agcatcatct gcacccccga ccacaagttc     1860

atgctgagag acggcagcta caagtgcgcc atggacctga ccctggacga cagcctgatg     1920

cccctgcaca gaaagatcag caccaccgag gacagcggcc acatggaggc cgtgctgaac     1980

tacaaccaca gaatcgtgaa catcgaggcc gtgagcgaga ccatcgacgt gtacgacatc     2040

gaggtgcccc acacccacaa cttcgccctg gccagccacc atcaccatca ccatggctgg     2100

agccaccccc agttcgagaa gtaggcggcc gctcgagtct agagggcccg tttaaacccg     2160

ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt     2220

gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat     2280

tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag     2340

caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc     2400

ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg     2460

cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc     2520

cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc     2580

ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct     2640

cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac     2700

ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac     2760

tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat     2820

ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg     2880

tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat     2940

gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc     3000

aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac     3060

tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact     3120

aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta     3180

gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc     3240

cattttcgga tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtctgtcga     3300

gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga     3360

agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag     3420

ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct     3480

cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc     3540

ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct     3600

gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg     3660

gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg     3720

cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc     3780

gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg     3840

gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac     3900

agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat     3960

cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag     4020

gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga     4080

ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg     4140

atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag     4200

aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg     4260

ccccagcact cgtccgaggg caaaggaata gcacgtgcta cgagatttcg attccaccgc     4320

cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct     4380

ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta     4440

taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact     4500

gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc     4560

gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta     4620

tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc     4680

ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg     4740

aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg     4800

tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg     4860

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa     4920

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc     4980

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc     5040

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag     5100

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct     5160

cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta     5220

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc     5280

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc     5340

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt     5400

gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct     5460

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     5520

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     5580

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     5640

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     5700

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg     5760

cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg     5820

actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc     5880

aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc     5940

cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa     6000

ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc     6060

cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg     6120

ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc     6180

cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat     6240

ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg     6300

tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc     6360

ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg     6420

aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat     6480

gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg     6540

gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg     6600

ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct     6660

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac     6720

atttccccga aaagtgccac ctgacgtc                                        6748


<210>  23
<211>  206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of sortase A from Staphylococcus aureus

<400>  23

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Phe 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Asp 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Lys Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  24
<211>  621
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding for SEQ ID NO 23

<400>  24
atgaaaaaat ggacaaatcg attaatgaca atcgctggtg tggtacttat cctagtggca       60

gcatatttgt ttgctaaacc acatatcgat aattatcttc acgataaaga taaagatgaa      120

aagattgaac aatatgataa aaatgtaaaa gaacaggcga gtaaagataa aaagcagcaa      180

gctaaacctc aaattccgaa agataaatcg aaagtggcag gctatattga aattccagat      240

gctgatatta aagaaccagt atatccagga ccagcaacac ctgaacaatt aaatagaggt      300

gtaagctttg cagaagaaaa tgaatcacta gatgatcaaa atatttcaat tgcaggacac      360

actttcattg accgtccgaa ctatcaattt acaaatctta aagcagccaa aaaaggtagt      420

atggtgtact ttaaagttgg taatgaaaca cgtaagtata aaatgacaag tataagagat      480

gttaagccta cagatgtagg agttctagat gaacaaaaag gtaaagataa acaattaaca      540

ttaattactt gtgatgatta caatgaaaag acaggcgttt gggaaaaacg taaaatcttt      600

gtagctacag aagtcaaata a                                                621


<210>  25
<211>  465
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  coding region for a 6xHis tagged version of Staphylococcus aureus
       sortase A (aa60-205)

<400>  25
atgcaagcta aacctcaaat tccgaaagat aaatcgaaag tggcaggcta tattgaaatt       60

ccagatgctg atattaaaga accagtatat ccaggaccag caacacctga acaattaaat      120

agaggtgtaa gctttgcaga agaaaatgaa tcactagatg atcaaaatat ttcaattgca      180

ggacacactt tcattgaccg tccgaactat caatttacaa atcttaaagc agccaaaaaa      240

ggtagtatgg tgtactttaa agttggtaat gaaacacgta agtataaaat gacaagtata      300

agagatgtta agcctacaga tgtaggagtt ctagatgaac aaaaaggtaa agataaacaa      360

ttaacattaa ttacttgtga tgattacaat gaaaagacag gcgtttggga aaaacgtaaa      420

atctttgtag ctacagaagt caaacaccat caccatcacc attaa                      465


<210>  26
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence translated from SEQ ID NO 25

<400>  26

Met Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly 
1               5                   10                  15      


Tyr Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly 
            20                  25                  30          


Pro Ala Thr Pro Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu 
        35                  40                  45              


Asn Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe 
    50                  55                  60                  


Ile Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys 
65                  70                  75                  80  


Gly Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys 
                85                  90                  95      


Met Thr Ser Ile Arg Asp Val Lys Pro Thr Asp Val Gly Val Leu Asp 
            100                 105                 110         


Glu Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp 
        115                 120                 125             


Tyr Asn Glu Lys Thr Gly Val Trp Glu Lys Arg Lys Ile Phe Val Ala 
    130                 135                 140                 


Thr Glu Val Lys His His His His His His 
145                 150                 


<210>  27
<211>  1401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Nucleotide sequence of mAb Ac10 Heavy Chain

<400>  27
atgaattttg gactgaggct gattttcctg gtgctgaccc tgaaaggcgt ccagtgtcag       60

atccagctgc agcagtctgg ccccgaggtc gtgaaacctg gcgcctccgt gaagatctcc      120

tgcaaggcct ccggctacac cttcaccgac tactacatca cctgggtcaa gcagaagccc      180

ggccagggcc tggaatggat cggctggatc tatcccggct ccggcaacac caagtacaac      240

gagaagttca agggcaaggc caccctgacc gtggacacct cctcttccac cgccttcatg      300

cagctgtcct ccctgacctc cgaggatacc gccgtgtact tctgcgccaa ctacggcaac      360

tattggttcg cctactgggg ccagggcaca caagtgaccg tgtctgctgc tagcaccaag      420

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcagcc      480

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc      540

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc      600

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac      660

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac      720

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc      780

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc      840

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc      900

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt      960

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc     1020

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg     1080

cagccccgag aaccacaggt gtacaccctg cccccatccc gggatgagct gaccaagaac     1140

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg     1200

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac     1260

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac     1320

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacaca gaagagcctc     1380

tccctgtctc cgggtaaata g                                               1401


<210>  28
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AMINO ACID SEQUENCE OF MAB AC10 HEAVY CHAIN

<400>  28

Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gln Ile Gln Leu Gln Gln Ser Gly Pro Glu Val Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Tyr Ile Thr Trp Val Lys Gln Lys Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Trp Ile Tyr Pro Gly Ser Gly Asn Thr Lys Tyr Asn 
65                  70                  75                  80  


Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser 
                85                  90                  95      


Thr Ala Phe Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Phe Cys Ala Asn Tyr Gly Asn Tyr Trp Phe Ala Tyr Trp Gly Gln 
        115                 120                 125             


Gly Thr Gln Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val 
    130                 135                 140                 


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
145                 150                 155                 160 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
                165                 170                 175     


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
            180                 185                 190         


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
        195                 200                 205             


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
    210                 215                 220                 


Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp 
225                 230                 235                 240 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
                245                 250                 255     


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
            260                 265                 270         


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
        275                 280                 285             


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
    290                 295                 300                 


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
305                 310                 315                 320 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
                325                 330                 335     


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
            340                 345                 350         


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
        355                 360                 365             


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
    370                 375                 380                 


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
385                 390                 395                 400 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
                405                 410                 415     


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
            420                 425                 430         


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
        435                 440                 445             


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
    450                 455                 460                 


Gly Lys 
465     


<210>  29
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Nucleotide sequence of mAb Ac10 Light Chain

<400>  29
atgaattttg gactgaggct gattttcctg gtgctgaccc tgaaaggcgt ccagtgtgac       60

attgtgctga cccaatctcc agcttctttg gctgtgtctc tagggcagag ggccaccatc      120

tcctgcaagg ccagccaaag tgttgatttt gatggtgata gttatatgaa ctggtaccaa      180

cagaaaccag gacagccacc caaagtcctc atctatgctg catccaatct agaatctggg      240

atcccagcca ggtttagtgg cagtgggtct gggacagact tcaccctcaa catccatcct      300

gtggaggagg aggatgctgc aacctattac tgtcagcaaa gtaatgagga tccgtggacg      360

ttcggtggag gcaccaagct ggaaatcaaa cgtacggtgg ctgcaccatc tgtcttcatc      420

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat      480

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt      540

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc      600

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc      660

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg ttag            714


<210>  30
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mAb Ac10 Light Chain

<400>  30

Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val 
        35                  40                  45              


Asp Phe Asp Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Gln Pro Pro Lys Val Leu Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly 
65                  70                  75                  80  


Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Asn Ile His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Ser Asn Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu 
        115                 120                 125             


Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser 
    130                 135                 140                 


Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn 
145                 150                 155                 160 


Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala 
                165                 170                 175     


Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys 
            180                 185                 190         


Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp 
        195                 200                 205             


Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu 
    210                 215                 220                 


Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235         


<210>  31
<211>  1422
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Nucleotide sequence of Trastuzumab Heavy Chain

<400>  31
atgaaaaaaa acattgcctt tctgctggcc tccatgttcg tgttctctat cgccacaaac       60

gcttacgctg aagtccagct ggtcgaatct ggtggtggcc tggtccagcc tggtggatca      120

ctgagactgt cctgtgctgc ttctggtttc aacatcaagg acacctacat ccattgggtc      180

agacaggcac ctggcaaggg actggaatgg gtcgcccgaa tctaccctac aaacggctac      240

actcgctacg ccgactccgt caagggacgc tttaccatct ccgccgacac ctctaaaaac      300

accgcctacc tgcagatgaa tagtctgagg gccgaggata ctgctgtgta ctactgctca      360

cgatggggag gcgacggctt ttacgctatg gattactggg gacagggaac tctggtcact      420

gtgtctagcg ctagcaccaa gggcccatcg gtcttccccc tggcaccctc ctccaagagc      480

acctctgggg gcacagcagc cctgggctgc ctggtcaagg actacttccc cgaaccggtg      540

acggtgtcgt ggaactcagg cgccctgacc agcggcgtgc acaccttccc ggctgtccta      600

cagtcctcag gactctactc cctcagcagc gtggtgaccg tgccctccag cagcttgggc      660

acccagacct acatctgcaa cgtgaatcac aagcccagca acaccaaggt ggacaagaaa      720

gttgagccca aatcttgtga caaaactcac acatgcccac cgtgcccagc acctgaactc      780

ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct catgatctcc      840

cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag      900

ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc gcgggaggag      960

cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca ggactggctg     1020

aatggcaagg agtacaagtg caaggtctcc aacaaagccc tcccagcccc catcgagaaa     1080

accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccct gcccccatcc     1140

cgggatgagc tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc     1200

agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta caagaccacg     1260

cctcccgtgc tggactccga cggctccttc ttcctctaca gcaagctcac cgtggacaag     1320

agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc tctgcacaac     1380

cactacacac agaagagcct ctccctgtct ccgggtaaat ag                        1422


<210>  32
<211>  473
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   Amino acid sequence of Trastuzumab Heavy Chain

<400>  32

Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala Glu Val Gln Leu Val Glu Ser Gly Gly 
            20                  25                  30          


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
        35                  40                  45              


Gly Phe Asn Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro 
    50                  55                  60                  


Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr 
65                  70                  75                  80  


Thr Arg Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp 
                85                  90                  95      


Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
            100                 105                 110         


Asp Thr Ala Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr 
        115                 120                 125             


Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala 
    130                 135                 140                 


Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
145                 150                 155                 160 


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
                165                 170                 175     


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
            180                 185                 190         


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
        195                 200                 205             


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
    210                 215                 220                 


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
225                 230                 235                 240 


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
                245                 250                 255     


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            260                 265                 270         


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        275                 280                 285             


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    290                 295                 300                 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
305                 310                 315                 320 


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                325                 330                 335     


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            340                 345                 350         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        355                 360                 365             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    370                 375                 380                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
385                 390                 395                 400 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                405                 410                 415     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            420                 425                 430         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        435                 440                 445             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    450                 455                 460                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
465                 470             


<210>  33
<211>  1491
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Nucleotide sequence of Trastuzumab Heavy Chain with C-terminal 
       GS peptide spacer, LPETG sortase A recognition motif and 
       additional 6x-His and strep-II affinity purification tags

<400>  33
atgaaaaaaa acattgcctt tctgctggcc tccatgttcg tgttctctat cgccacaaac       60

gcttacgctg aagtccagct ggtcgaatct ggtggtggcc tggtccagcc tggtggatca      120

ctgagactgt cctgtgctgc ttctggtttc aacatcaagg acacctacat ccattgggtc      180

agacaggcac ctggcaaggg actggaatgg gtcgcccgaa tctaccctac aaacggctac      240

actcgctacg ccgactccgt caagggacgc tttaccatct ccgccgacac ctctaaaaac      300

accgcctacc tgcagatgaa tagtctgagg gccgaggata ctgctgtgta ctactgctca      360

cgatggggag gcgacggctt ttacgctatg gattactggg gacagggaac tctggtcact      420

gtgtctagcg ctagcaccaa gggcccatcg gtcttccccc tggcaccctc ctccaagagc      480

acctctgggg gcacagcagc cctgggctgc ctggtcaagg actacttccc cgaaccggtg      540

acggtgtcgt ggaactcagg cgccctgacc agcggcgtgc acaccttccc ggctgtccta      600

cagtcctcag gactctactc cctcagcagc gtggtgaccg tgccctccag cagcttgggc      660

acccagacct acatctgcaa cgtgaatcac aagcccagca acaccaaggt ggacaagaaa      720

gttgagccca aatcttgtga caaaactcac acatgcccac cgtgcccagc acctgaactc      780

ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct catgatctcc      840

cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag      900

ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc gcgggaggag      960

cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca ggactggctg     1020

aatggcaagg agtacaagtg caaggtctcc aacaaagccc tcccagcccc catcgagaaa     1080

accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccct gcccccatcc     1140

cgggatgagc tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc     1200

agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta caagaccacg     1260

cctcccgtgc tggactccga cggctccttc ttcctctaca gcaagctcac cgtggacaag     1320

agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc tctgcacaac     1380

cactacacac agaagagcct ctccctgtct ccgggtaaag gatccctgcc cgagaccggc     1440

ggacaccatc accatcacca tggctggagc cacccccagt tcgagaagta g              1491


<210>  34
<211>  496
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   Amino acid sequence of Trastuzumab Heavy Chain with C-terminal 
       GS peptide spacer, LPETG sortase A recognition motif and 
       additional 6x-His and strep-II affinity purification tags

<400>  34

Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala Glu Val Gln Leu Val Glu Ser Gly Gly 
            20                  25                  30          


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
        35                  40                  45              


Gly Phe Asn Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro 
    50                  55                  60                  


Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr 
65                  70                  75                  80  


Thr Arg Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp 
                85                  90                  95      


Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
            100                 105                 110         


Asp Thr Ala Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr 
        115                 120                 125             


Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala 
    130                 135                 140                 


Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
145                 150                 155                 160 


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
                165                 170                 175     


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
            180                 185                 190         


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
        195                 200                 205             


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
    210                 215                 220                 


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
225                 230                 235                 240 


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
                245                 250                 255     


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            260                 265                 270         


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        275                 280                 285             


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    290                 295                 300                 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
305                 310                 315                 320 


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                325                 330                 335     


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            340                 345                 350         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        355                 360                 365             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    370                 375                 380                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
385                 390                 395                 400 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                405                 410                 415     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            420                 425                 430         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        435                 440                 445             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    450                 455                 460                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Ser Leu Pro Glu Thr Gly 
465                 470                 475                 480 


Gly His His His His His His Gly Trp Ser His Pro Gln Phe Glu Lys 
                485                 490                 495     


<210>  35
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of Trastuzumab Light Chain

<400>  35
atgaaaaaaa acattgcctt tctgctggcc tccatgttcg tgttctctat cgccacaaat       60

gcttatgccg acatccagat gacacagtct ccctcttccc tgtccgcttc tgtgggcgat      120

cgagtgacaa tcacctgtag ggctagtcag gatgtgaata ctgctgttgc ttggtaccag      180

cagaaaccag gaaaagcccc taaactgctg atctactctg cctcattcct gtactctggg      240

gtgccttctc gattcagtgg ttctagatct ggcaccgatt tcacactgac catttcttca      300

ctgcaacctg aggattttgc cacctactac tgtcagcagc actacacaac acctcccaca      360

tttggccagg gcacaaaagt ggagatcaaa cgtacggtgg ctgcaccatc tgtcttcatc      420

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat      480

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt      540

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc      600

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc      660

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg ttag            714


<210>  36
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of Trastuzumab Light Chain

<400>  36

Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala Asp Ile Gln Met Thr Gln Ser Pro Ser 
            20                  25                  30          


Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala 
        35                  40                  45              


Ser Gln Asp Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly 
65                  70                  75                  80  


Val Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln His Tyr Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu 
        115                 120                 125             


Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser 
    130                 135                 140                 


Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn 
145                 150                 155                 160 


Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala 
                165                 170                 175     


Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys 
            180                 185                 190         


Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp 
        195                 200                 205             


Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu 
    210                 215                 220                 


Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235         


<210>  37
<211>  783
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Nucleotide sequence of Trastuzumab Light Chain with C-terminal 
       GS peptide spacer, LPETG sortase A recognition motif and 
       additional 6x-His and strep-II affinity purification tags

<400>  37
atgaaaaaaa acattgcctt tctgctggcc tccatgttcg tgttctctat cgccacaaat       60

gcttatgccg acatccagat gacacagtct ccctcttccc tgtccgcttc tgtgggcgat      120

cgagtgacaa tcacctgtag ggctagtcag gatgtgaata ctgctgttgc ttggtaccag      180

cagaaaccag gaaaagcccc taaactgctg atctactctg cctcattcct gtactctggg      240

gtgccttctc gattcagtgg ttctagatct ggcaccgatt tcacactgac catttcttca      300

ctgcaacctg aggattttgc cacctactac tgtcagcagc actacacaac acctcccaca      360

tttggccagg gcacaaaagt ggagatcaaa cgtacggtgg ctgcaccatc tgtcttcatc      420

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat      480

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt      540

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc      600

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc      660

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg tggatccctg      720

cccgagaccg gcggacacca tcaccatcac catggctgga gccaccccca gttcgagaag      780

tag                                                                    783


<210>  38
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   Amino acid sequence of Trastuzumab Light Chain with C-terminal 
       GS peptide-spacer, LPETG sortase A recognition motif and 
       additional 6x-His and strep-II affinity purification tags

<400>  38

Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala Asp Ile Gln Met Thr Gln Ser Pro Ser 
            20                  25                  30          


Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala 
        35                  40                  45              


Ser Gln Asp Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly 
65                  70                  75                  80  


Val Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln His Tyr Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu 
        115                 120                 125             


Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser 
    130                 135                 140                 


Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn 
145                 150                 155                 160 


Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala 
                165                 170                 175     


Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys 
            180                 185                 190         


Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp 
        195                 200                 205             


Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu 
    210                 215                 220                 


Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Ser Leu 
225                 230                 235                 240 


Pro Glu Thr Gly Gly His His His His His His Gly Trp Ser His Pro 
                245                 250                 255     


Gln Phe Glu Lys 
            260 


<210>  39
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of mAb Ac10 Heavy Chain with C-terminal GS 
       peptide-spacer, LPETG sortase A recognition motif and additional 
       6x-His and strep-II affinity purification tags

<400>  39
atgaattttg gactgaggct gattttcctg gtgctgaccc tgaaaggcgt ccagtgtcag       60

atccagctgc agcagtctgg ccccgaggtc gtgaaacctg gcgcctccgt gaagatctcc      120

tgcaaggcct ccggctacac cttcaccgac tactacatca cctgggtcaa gcagaagccc      180

ggccagggcc tggaatggat cggctggatc tatcccggct ccggcaacac caagtacaac      240

gagaagttca agggcaaggc caccctgacc gtggacacct cctcttccac cgccttcatg      300

cagctgtcct ccctgacctc cgaggatacc gccgtgtact tctgcgccaa ctacggcaac      360

tattggttcg cctactgggg ccagggcaca caagtgaccg tgtctgctgc tagcaccaag      420

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcagcc      480

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc      540

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc      600

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac      660

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac      720

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc      780

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc      840

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc      900

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt      960

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc     1020

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg     1080

cagccccgag aaccacaggt gtacaccctg cccccatccc gggatgagct gaccaagaac     1140

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg     1200

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac     1260

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac     1320

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacaca gaagagcctc     1380

tccctgtctc cgggtaaagg atccctgccc gagaccggcg gacaccatca ccatcaccat     1440

ggctggagcc acccccagtt cgagaagtag                                      1470


<210>  40
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mAb Ac10 Heavy Chain with C-terminal GS 
       peptide-spacer, LPETG sortase A recognition motif and additional 
       6x-His and strep II affinity purification tags

<400>  40

Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gln Ile Gln Leu Gln Gln Ser Gly Pro Glu Val Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Tyr Ile Thr Trp Val Lys Gln Lys Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Trp Ile Tyr Pro Gly Ser Gly Asn Thr Lys Tyr Asn 
65                  70                  75                  80  


Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser 
                85                  90                  95      


Thr Ala Phe Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Phe Cys Ala Asn Tyr Gly Asn Tyr Trp Phe Ala Tyr Trp Gly Gln 
        115                 120                 125             


Gly Thr Gln Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val 
    130                 135                 140                 


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
145                 150                 155                 160 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
                165                 170                 175     


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
            180                 185                 190         


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
        195                 200                 205             


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
    210                 215                 220                 


Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp 
225                 230                 235                 240 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
                245                 250                 255     


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
            260                 265                 270         


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
        275                 280                 285             


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
    290                 295                 300                 


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
305                 310                 315                 320 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
                325                 330                 335     


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
            340                 345                 350         


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
        355                 360                 365             


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
    370                 375                 380                 


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
385                 390                 395                 400 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
                405                 410                 415     


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
            420                 425                 430         


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
        435                 440                 445             


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
    450                 455                 460                 


Gly Lys Gly Ser Leu Pro Glu Thr Gly Gly His His His His His His 
465                 470                 475                 480 


Gly Trp Ser His Pro Gln Phe Glu Lys 
                485                 


<210>  41
<211>  783
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of mAb Ac10 Light Chain with C-terminal GS 
       peptide-spacer, LPETG sortase A recognition motif and additional 
       6x-His and strep II affinity purification tags

<400>  41
atgaattttg gactgaggct gattttcctg gtgctgaccc tgaaaggcgt ccagtgtgac       60

attgtgctga cccaatctcc agcttctttg gctgtgtctc tagggcagag ggccaccatc      120

tcctgcaagg ccagccaaag tgttgatttt gatggtgata gttatatgaa ctggtaccaa      180

cagaaaccag gacagccacc caaagtcctc atctatgctg catccaatct agaatctggg      240

atcccagcca ggtttagtgg cagtgggtct gggacagact tcaccctcaa catccatcct      300

gtggaggagg aggatgctgc aacctattac tgtcagcaaa gtaatgagga tccgtggacg      360

ttcggtggag gcaccaagct ggaaatcaaa cgtacggtgg ctgcaccatc tgtcttcatc      420

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat      480

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt      540

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc      600

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc      660

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg tggatccctg      720

cccgagaccg gcggacacca tcaccatcac catggctgga gccaccccca gttcgagaag      780

tag                                                                    783


<210>  42
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mAb Ac10 Light Chain with C-terminal GS 
       peptide-spacer, LPETG sortase A recognition motif and additional 
       6x-His and strep II affinity purification tags

<400>  42

Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val 
        35                  40                  45              


Asp Phe Asp Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Gln Pro Pro Lys Val Leu Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly 
65                  70                  75                  80  


Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Asn Ile His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Ser Asn Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu 
        115                 120                 125             


Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser 
    130                 135                 140                 


Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn 
145                 150                 155                 160 


Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala 
                165                 170                 175     


Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys 
            180                 185                 190         


Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp 
        195                 200                 205             


Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu 
    210                 215                 220                 


Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Ser Leu 
225                 230                 235                 240 


Pro Glu Thr Gly Gly His His His His His His Gly Trp Ser His Pro 
                245                 250                 255     


Gln Phe Glu Lys 
            260 


<210>  43
<211>  1443
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of mAb Ac10 Heavy Chain with C-terminal LPETG
       sortase A recognition motif and strep II affinity purification 
       tag

<400>  43
atgaattttg gactgaggct gattttcctg gtgctgaccc tgaaaggcgt ccagtgtcag       60

atccagctgc agcagtctgg ccccgaggtc gtgaaacctg gcgcctccgt gaagatctcc      120

tgcaaggcct ccggctacac cttcaccgac tactacatca cctgggtcaa gcagaagccc      180

ggccagggcc tggaatggat cggctggatc tatcccggct ccggcaacac caagtacaac      240

gagaagttca agggcaaggc caccctgacc gtggacacct cctcttccac cgccttcatg      300

cagctgtcct ccctgacctc cgaggatacc gccgtgtact tctgcgccaa ctacggcaac      360

tattggttcg cctactgggg ccagggcaca caagtgaccg tgtctgctgc tagcaccaag      420

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcagcc      480

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc      540

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc      600

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac      660

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac      720

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc      780

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc      840

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc      900

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt      960

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc     1020

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg     1080

cagccccgag aaccacaggt gtacaccctg cccccatccc gggatgagct gaccaagaac     1140

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg     1200

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac     1260

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac     1320

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacaca gaagagcctc     1380

tccctgtctc cgggtaaact gcccgagacc ggcggatgga gccaccccca gttcgagaag     1440

tag                                                                   1443


<210>  44
<211>  480
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mAb Ac10 Heavy Chain with C-terminal LPETG
       sortase A recognition motif and strep-II affinity purification 
       tag

<400>  44

Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gln Ile Gln Leu Gln Gln Ser Gly Pro Glu Val Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Tyr Ile Thr Trp Val Lys Gln Lys Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Trp Ile Tyr Pro Gly Ser Gly Asn Thr Lys Tyr Asn 
65                  70                  75                  80  


Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser 
                85                  90                  95      


Thr Ala Phe Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Phe Cys Ala Asn Tyr Gly Asn Tyr Trp Phe Ala Tyr Trp Gly Gln 
        115                 120                 125             


Gly Thr Gln Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val 
    130                 135                 140                 


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
145                 150                 155                 160 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
                165                 170                 175     


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
            180                 185                 190         


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
        195                 200                 205             


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
    210                 215                 220                 


Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp 
225                 230                 235                 240 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
                245                 250                 255     


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
            260                 265                 270         


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
        275                 280                 285             


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
    290                 295                 300                 


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
305                 310                 315                 320 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
                325                 330                 335     


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
            340                 345                 350         


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
        355                 360                 365             


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
    370                 375                 380                 


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
385                 390                 395                 400 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
                405                 410                 415     


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
            420                 425                 430         


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
        435                 440                 445             


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
    450                 455                 460                 


Gly Lys Leu Pro Glu Thr Gly Gly Trp Ser His Pro Gln Phe Glu Lys 
465                 470                 475                 480 


<210>  45
<211>  756
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of mAb Ac10 Light Chain with C-terminal LPETG
       sortase A recognition motif and strep-II affinity purification 
       tag

<400>  45
atgaattttg gactgaggct gattttcctg gtgctgaccc tgaaaggcgt ccagtgtgac       60

attgtgctga cccaatctcc agcttctttg gctgtgtctc tagggcagag ggccaccatc      120

tcctgcaagg ccagccaaag tgttgatttt gatggtgata gttatatgaa ctggtaccaa      180

cagaaaccag gacagccacc caaagtcctc atctatgctg catccaatct agaatctggg      240

atcccagcca ggtttagtgg cagtgggtct gggacagact tcaccctcaa catccatcct      300

gtggaggagg aggatgctgc aacctattac tgtcagcaaa gtaatgagga tccgtggacg      360

ttcggtggag gcaccaagct ggaaatcaaa cgtacggtgg ctgcaccatc tgtcttcatc      420

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat      480

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt      540

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc      600

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc      660

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg tctgcccgag      720

accggcggat ggagccaccc ccagttcgag aagtag                                756


<210>  46
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mAb Ac10 Light Chain with C-terminal LPETG
       sortase A recognition motif and strep-II affinity purification 
       tag

<400>  46

Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val 
        35                  40                  45              


Asp Phe Asp Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Gln Pro Pro Lys Val Leu Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly 
65                  70                  75                  80  


Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Asn Ile His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Ser Asn Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu 
        115                 120                 125             


Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser 
    130                 135                 140                 


Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn 
145                 150                 155                 160 


Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala 
                165                 170                 175     


Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys 
            180                 185                 190         


Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp 
        195                 200                 205             


Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu 
    210                 215                 220                 


Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Leu Pro Glu 
225                 230                 235                 240 


Thr Gly Gly Trp Ser His Pro Gln Phe Glu Lys 
                245                 250     


<210>  47
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of mAb Ac10 Light Chain with C-terminal GS 
       peptide-spacer, LPETG sortase A recognition motif and strep II 
       affinity purification tag

<400>  47
atgaattttg gactgaggct gattttcctg gtgctgaccc tgaaaggcgt ccagtgtgac       60

attgtgctga cccaatctcc agcttctttg gctgtgtctc tagggcagag ggccaccatc      120

tcctgcaagg ccagccaaag tgttgatttt gatggtgata gttatatgaa ctggtaccaa      180

cagaaaccag gacagccacc caaagtcctc atctatgctg catccaatct agaatctggg      240

atcccagcca ggtttagtgg cagtgggtct gggacagact tcaccctcaa catccatcct      300

gtggaggagg aggatgctgc aacctattac tgtcagcaaa gtaatgagga tccgtggacg      360

ttcggtggag gcaccaagct ggaaatcaaa cgtacggtgg ctgcaccatc tgtcttcatc      420

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat      480

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt      540

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc      600

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc      660

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg tggatccctg      720

cccgagaccg gcggatggag ccacccccag ttcgagaagt ag                         762


<210>  48
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mAb Ac10 Light Chain with C-terminal GS 
       peptide-spacer, LPETG sortase A recognition motif and strep II 
       affinity purification tag

<400>  48

Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val 
        35                  40                  45              


Asp Phe Asp Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Gln Pro Pro Lys Val Leu Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly 
65                  70                  75                  80  


Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Asn Ile His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Ser Asn Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu 
        115                 120                 125             


Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser 
    130                 135                 140                 


Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn 
145                 150                 155                 160 


Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala 
                165                 170                 175     


Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys 
            180                 185                 190         


Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp 
        195                 200                 205             


Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu 
    210                 215                 220                 


Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Ser Leu 
225                 230                 235                 240 


Pro Glu Thr Gly Gly Trp Ser His Pro Gln Phe Glu Lys 
                245                 250             


<210>  49
<211>  765
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of mAb Ac10 Light Chain with C-terminal GGS 
       peptide-spacer, LPETG sortase A recognition motif and strep II 
       affinity purification tag

<400>  49
atgaattttg gactgaggct gattttcctg gtgctgaccc tgaaaggcgt ccagtgtgac       60

attgtgctga cccaatctcc agcttctttg gctgtgtctc tagggcagag ggccaccatc      120

tcctgcaagg ccagccaaag tgttgatttt gatggtgata gttatatgaa ctggtaccaa      180

cagaaaccag gacagccacc caaagtcctc atctatgctg catccaatct agaatctggg      240

atcccagcca ggtttagtgg cagtgggtct gggacagact tcaccctcaa catccatcct      300

gtggaggagg aggatgctgc aacctattac tgtcagcaaa gtaatgagga tccgtggacg      360

ttcggtggag gcaccaagct ggaaatcaaa cgtacggtgg ctgcaccatc tgtcttcatc      420

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat      480

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt      540

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc      600

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc      660

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg tggtggatcc      720

ctgcccgaga ccggcggatg gagccacccc cagttcgaga agtag                      765


<210>  50
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mAb Ac10 Light Chain with C-terminal GGS 
       peptide-spacer, LPETG sortase A recognition motif and strep II 
       affinity purification tag

<400>  50

Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val 
        35                  40                  45              


Asp Phe Asp Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Gln Pro Pro Lys Val Leu Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly 
65                  70                  75                  80  


Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Asn Ile His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Ser Asn Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu 
        115                 120                 125             


Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser 
    130                 135                 140                 


Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn 
145                 150                 155                 160 


Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala 
                165                 170                 175     


Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys 
            180                 185                 190         


Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp 
        195                 200                 205             


Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu 
    210                 215                 220                 


Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Gly Ser 
225                 230                 235                 240 


Leu Pro Glu Thr Gly Gly Trp Ser His Pro Gln Phe Glu Lys 
                245                 250                 


<210>  51
<211>  768
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of mAb Ac10 Light Chain with C-terminal GGGS 
       peptide-spacer, LPETG sortase A recognition motif and strep II 
       affinity purification tag

<400>  51
atgaattttg gactgaggct gattttcctg gtgctgaccc tgaaaggcgt ccagtgtgac       60

attgtgctga cccaatctcc agcttctttg gctgtgtctc tagggcagag ggccaccatc      120

tcctgcaagg ccagccaaag tgttgatttt gatggtgata gttatatgaa ctggtaccaa      180

cagaaaccag gacagccacc caaagtcctc atctatgctg catccaatct agaatctggg      240

atcccagcca ggtttagtgg cagtgggtct gggacagact tcaccctcaa catccatcct      300

gtggaggagg aggatgctgc aacctattac tgtcagcaaa gtaatgagga tccgtggacg      360

ttcggtggag gcaccaagct ggaaatcaaa cgtacggtgg ctgcaccatc tgtcttcatc      420

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat      480

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt      540

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc      600

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc      660

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg tgggggtgga      720

tccctgcccg agaccggcgg atggagccac ccccagttcg agaagtag                   768


<210>  52
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mAb Ac10 Light Chain with C-terminal GGGS 
       peptide-spacer, LPETG sortase A recognition motif and strep II 
       affinity purification tag

<400>  52

Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val 
        35                  40                  45              


Asp Phe Asp Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Gln Pro Pro Lys Val Leu Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly 
65                  70                  75                  80  


Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Asn Ile His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Ser Asn Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu 
        115                 120                 125             


Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser 
    130                 135                 140                 


Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn 
145                 150                 155                 160 


Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala 
                165                 170                 175     


Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys 
            180                 185                 190         


Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp 
        195                 200                 205             


Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu 
    210                 215                 220                 


Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Gly Gly 
225                 230                 235                 240 


Ser Leu Pro Glu Thr Gly Gly Trp Ser His Pro Gln Phe Glu Lys 
                245                 250                 255 


<210>  53
<211>  771
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of mAb Ac10 Light Chain with C-terminal GGGGS
       peptide-spacer, LPETG sortase A recognition motif and strep II 
       affinity purification tag

<400>  53
atgaattttg gactgaggct gattttcctg gtgctgaccc tgaaaggcgt ccagtgtgac       60

attgtgctga cccaatctcc agcttctttg gctgtgtctc tagggcagag ggccaccatc      120

tcctgcaagg ccagccaaag tgttgatttt gatggtgata gttatatgaa ctggtaccaa      180

cagaaaccag gacagccacc caaagtcctc atctatgctg catccaatct agaatctggg      240

atcccagcca ggtttagtgg cagtgggtct gggacagact tcaccctcaa catccatcct      300

gtggaggagg aggatgctgc aacctattac tgtcagcaaa gtaatgagga tccgtggacg      360

ttcggtggag gcaccaagct ggaaatcaaa cgtacggtgg ctgcaccatc tgtcttcatc      420

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat      480

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt      540

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc      600

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc      660

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg tggagggggt      720

ggatccctgc ccgagaccgg cggatggagc cacccccagt tcgagaagta g               771


<210>  54
<211>  256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mAb Ac10 Light Chain with C-terminal GGGGS
       peptide-spacer, LPETG sortase A recognition motif and strep II 
       affinity purification tag

<400>  54

Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val 
        35                  40                  45              


Asp Phe Asp Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Gln Pro Pro Lys Val Leu Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly 
65                  70                  75                  80  


Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Asn Ile His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Ser Asn Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu 
        115                 120                 125             


Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser 
    130                 135                 140                 


Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn 
145                 150                 155                 160 


Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala 
                165                 170                 175     


Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys 
            180                 185                 190         


Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp 
        195                 200                 205             


Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu 
    210                 215                 220                 


Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Gly Gly 
225                 230                 235                 240 


Gly Ser Leu Pro Glu Thr Gly Gly Trp Ser His Pro Gln Phe Glu Lys 
                245                 250                 255     


<210>  55
<211>  1464
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of Trastuzumab Heavy Chain with C-terminal 
       LPETG Sortase tag and strep II affinity purification tag

<400>  55
atgaaaaaaa acattgcctt tctgctggcc tccatgttcg tgttctctat cgccacaaac       60

gcttacgctg aagtccagct ggtcgaatct ggtggtggcc tggtccagcc tggtggatca      120

ctgagactgt cctgtgctgc ttctggtttc aacatcaagg acacctacat ccattgggtc      180

agacaggcac ctggcaaggg actggaatgg gtcgcccgaa tctaccctac aaacggctac      240

actcgctacg ccgactccgt caagggacgc tttaccatct ccgccgacac ctctaaaaac      300

accgcctacc tgcagatgaa tagtctgagg gccgaggata ctgctgtgta ctactgctca      360

cgatggggag gcgacggctt ttacgctatg gattactggg gacagggaac tctggtcact      420

gtgtctagcg ctagcaccaa gggcccatcg gtcttccccc tggcaccctc ctccaagagc      480

acctctgggg gcacagcagc cctgggctgc ctggtcaagg actacttccc cgaaccggtg      540

acggtgtcgt ggaactcagg cgccctgacc agcggcgtgc acaccttccc ggctgtccta      600

cagtcctcag gactctactc cctcagcagc gtggtgaccg tgccctccag cagcttgggc      660

acccagacct acatctgcaa cgtgaatcac aagcccagca acaccaaggt ggacaagaaa      720

gttgagccca aatcttgtga caaaactcac acatgcccac cgtgcccagc acctgaactc      780

ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct catgatctcc      840

cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag      900

ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc gcgggaggag      960

cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca ggactggctg     1020

aatggcaagg agtacaagtg caaggtctcc aacaaagccc tcccagcccc catcgagaaa     1080

accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccct gcccccatcc     1140

cgggatgagc tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc     1200

agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta caagaccacg     1260

cctcccgtgc tggactccga cggctccttc ttcctctaca gcaagctcac cgtggacaag     1320

agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc tctgcacaac     1380

cactacacac agaagagcct ctccctgtct ccgggtaaac tgcccgagac cggcggatgg     1440

agccaccccc agttcgagaa gtag                                            1464


<210>  56
<211>  487
<212>  PRT
<213>  Amino acid sequence of mAb Ac10 Heavy Chain with C-terminal LPETG Sortase tag and strep II affinity purification tag

<400>  56

Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala Glu Val Gln Leu Val Glu Ser Gly Gly 
            20                  25                  30          


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
        35                  40                  45              


Gly Phe Asn Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro 
    50                  55                  60                  


Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr 
65                  70                  75                  80  


Thr Arg Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp 
                85                  90                  95      


Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
            100                 105                 110         


Asp Thr Ala Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr 
        115                 120                 125             


Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala 
    130                 135                 140                 


Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
145                 150                 155                 160 


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
                165                 170                 175     


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
            180                 185                 190         


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
        195                 200                 205             


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
    210                 215                 220                 


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys 
225                 230                 235                 240 


Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
                245                 250                 255     


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            260                 265                 270         


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        275                 280                 285             


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    290                 295                 300                 


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
305                 310                 315                 320 


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                325                 330                 335     


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            340                 345                 350         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        355                 360                 365             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    370                 375                 380                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
385                 390                 395                 400 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                405                 410                 415     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            420                 425                 430         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        435                 440                 445             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    450                 455                 460                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys Leu Pro Glu Thr Gly Gly Trp 
465                 470                 475                 480 


Ser His Pro Gln Phe Glu Lys 
                485         


<210>  57
<211>  771
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of Trastuzumab Light Chain with C-terminal 
       GGGGS linker, LPETG Sortase tag and strep II affinity 
       purification tag

<400>  57
atgaaaaaaa acattgcctt tctgctggcc tccatgttcg tgttctctat cgccacaaat       60

gcttatgccg acatccagat gacacagtct ccctcttccc tgtccgcttc tgtgggcgat      120

cgagtgacaa tcacctgtag ggctagtcag gatgtgaata ctgctgttgc ttggtaccag      180

cagaaaccag gaaaagcccc taaactgctg atctactctg cctcattcct gtactctggg      240

gtgccttctc gattcagtgg ttctagatct ggcaccgatt tcacactgac catttcttca      300

ctgcaacctg aggattttgc cacctactac tgtcagcagc actacacaac acctcccaca      360

tttggccagg gcacaaaagt ggagatcaaa cgtacggtgg ctgcaccatc tgtcttcatc      420

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat      480

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt      540

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc      600

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc      660

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg tggagggggt      720

ggatccctgc ccgagaccgg cggatggagc cacccccagt tcgagaagta g               771


<210>  58
<211>  256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mAb Ac10 Light Chain with C-terminal GGGGS
       linker, LPETG Sortase tag and strep II affinity purification tag

<400>  58

Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala Asp Ile Gln Met Thr Gln Ser Pro Ser 
            20                  25                  30          


Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala 
        35                  40                  45              


Ser Gln Asp Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly 
65                  70                  75                  80  


Val Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln His Tyr Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu 
        115                 120                 125             


Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser 
    130                 135                 140                 


Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn 
145                 150                 155                 160 


Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala 
                165                 170                 175     


Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys 
            180                 185                 190         


Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp 
        195                 200                 205             


Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu 
    210                 215                 220                 


Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Gly Gly Gly 
225                 230                 235                 240 


Gly Ser Leu Pro Glu Thr Gly Gly Trp Ser His Pro Gln Phe Glu Lys 
                245                 250                 255     


