                         SEQUENCE LISTING

<110>  WANG, Qinghua
 
<120>  A GROWTH HORMONE SECRETAGOGUE RECEPTOR BASED PROTEIN, NUCLEIC 
       ACIDS AND METHODS AND USES THEREOF

<130>  12960-P43662PC00

<140>  PCT/CA2014/000271
<141>  2014-03-14

<150>  61/784,267
<151>  2013-03-14

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  981
<212>  DNA
<213>  Synthetic Construct

<400>  1
atggatgcca tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt       60

tcgaacagcg agagcgacac gcgtcggaaa cggatgtgga acgcgacgcc cagcgaggag      120

ccggagccta acgtcacgct ggacctggac tgggacgctt ctcccggcaa cgactcactc      180

tctgacgaac tgctgccact gttccccgcg cgcctctggc agtatcggcc ctggaacttc      240

ggcgacctgc tctgcaaact cttccagttt gtcagcgaga gctgcaccgt gggcgtggag      300

cacgagaacg gcacagatcc ccgggacacc aacgagtgcc gcgccaccga gttcgctgtg      360

cgctctggcg gcggcggacc cagcgagacc gtcacctgca acgttgccca cccggccagc      420

agcaccaagg tggacaagaa aattgtgccc agggattgtg gttgtaagcc ttgcatatgt      480

acagtcccag aagtatcatc tgtcttcatc ttccccccaa agcccaagga tgtgctcacc      540

attactctga ctcctaaggt cacgtgtgtt gtggtagaca tcagcaagga tgatcccgag      600

gtccagttca gctggtttgt agatgatgtg gaggtgcaca cagctcagac gcaaccccgg      660

gaggagcagt tcaacagcac tttccgctca gtcagtgaac ttcccatcat gcaccaggac      720

tggctcaatg gcaaggagtt caaatgcagg gtcaacagtg cagctttccc tgcccccatc      780

gagaaaacca tctccaaaac caaaggcaga ccgaaggctc cacaggtgta caccattcca      840

cctcccaagg agcagatggc caaggataaa gtcagtctga cctgcatgat aacagacttc      900

ttccctgctc gaggagggcc cgaacaaaaa ctcatctcag aagaggatct gaatagcgcc      960

gtcgaccatc atcatcatca t                                                981


<210>  2
<211>  368
<212>  PRT
<213>  Synthetic Construct


<220>
<221>  SITE
<222>  (355)..(356)
<223>  Stop Codons

<400>  2

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Asn Ser Glu Ser Asp Thr Arg Arg Lys Arg Met 
            20                  25                  30          


Trp Asn Ala Thr Pro Ser Glu Glu Pro Glu Pro Asn Val Thr Leu Asp 
        35                  40                  45              


Leu Asp Trp Asp Ala Ser Pro Gly Asn Asp Ser Leu Ser Asp Glu Leu 
    50                  55                  60                  


Leu Pro Leu Phe Pro Ala Arg Leu Trp Gln Tyr Arg Pro Trp Asn Phe 
65                  70                  75                  80  


Gly Asp Leu Leu Cys Lys Leu Phe Gln Phe Val Ser Glu Ser Cys Thr 
                85                  90                  95      


Val Gly Val Glu His Glu Asn Gly Thr Asp Pro Arg Asp Thr Asn Glu 
            100                 105                 110         


Cys Arg Ala Thr Glu Phe Ala Val Arg Ser Gly Gly Gly Gly Ser Arg 
        115                 120                 125             


Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly 
    130                 135                 140                 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Pro Leu Met Ile 
145                 150                 155                 160 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
                165                 170                 175     


Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
            180                 185                 190         


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg 
        195                 200                 205             


Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys 
    210                 215                 220                 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu 
225                 230                 235                 240 


Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
                245                 250                 255     


Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
            260                 265                 270         


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
        275                 280                 285             


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met 
    290                 295                 300                 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
305                 310                 315                 320 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
                325                 330                 335     


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
            340                 345                 350         


Gly Lys Xaa Xaa Arg Pro Arg Ile Pro Arg Pro Arg Pro Leu Ala Asn 
        355                 360                 365             


<210>  3
<211>  117
<212>  DNA
<213>  Mus musculus

<400>  3
atgtggaacg cgacgcccag cgaggagccg gagcctaacg tcacgctgga cctggactgg       60

gacgcttctc ccggcaacga ctcactctct gacgaactgc tgccactgtt ccccgcg         117


<210>  4
<211>  39
<212>  PRT
<213>  Mus Musculus

<400>  4

Met Trp Asn Ala Thr Pro Ser Glu Glu Pro Glu Pro Asn Val Thr Leu 
1               5                   10                  15      


Asp Leu Asp Trp Asp Ala Ser Pro Gly Asn Asp Ser Leu Ser Asp Glu 
            20                  25                  30          


Leu Leu Pro Leu Phe Pro Ala 
        35                  


<210>  5
<211>  78
<212>  DNA
<213>  Mus Musculus

<400>  5
cgcctctggc agtatcggcc ctggaacttc ggcgacctgc tctgcaaact cttccagttt       60

gtcagcgaga gctgcacc                                                     78


<210>  6
<211>  26
<212>  PRT
<213>  Mus Musculus

<400>  6

Arg Leu Trp Gln Tyr Arg Pro Trp Asn Phe Gly Asp Leu Leu Cys Lys 
1               5                   10                  15      


Leu Phe Gln Phe Val Ser Glu Ser Cys Thr 
            20                  25      


<210>  7
<211>  78
<212>  DNA
<213>  Mus Musculus

<400>  7
gtgggcgtgg agcacgagaa cggcacagat ccccgggaca ccaacgagtg ccgcgccacc       60

gagttcgctg tgcgctct                                                     78


<210>  8
<211>  26
<212>  PRT
<213>  Mus Musculus

<400>  8

Val Gly Val Glu His Glu Asn Gly Thr Asp Pro Arg Asp Thr Asn Glu 
1               5                   10                  15      


Cys Arg Ala Thr Glu Phe Ala Val Arg Ser 
            20                  25      


<210>  9
<211>  1095
<212>  DNA
<213>  Mus Musculus

<400>  9
atgtggaacg cgacgcccag cgaggagccg gagcctaacg tcacgctgga cctggactgg       60

gacgcttctc ccggcaacga ctcactctct gacgaactgc tgccactgtt ccccgcgccg      120

ctgctggcgg gcgtcactgc cacctgcgtg gcgctcttcg tggtgggcat ctcgggcaac      180

ctgctcacca tgctggtggt gtcccgcttc cgcgagctgc gcaccaccac caacctctac      240

ctatccagca tggccttctc cgatctgctc atcttcctgt gcatgccgct ggacctcgtc      300

cgcctctggc agtatcggcc ctggaacttc ggcgacctgc tctgcaaact cttccagttt      360

gtcagcgaga gctgcaccta cgccacggtc ctcaccatca ccgcgctgag cgtcgagcgc      420

tacttcgcca tctgcttccc gctgcgggcc aaggtggtgg tcaccaaggg ccgtgtgaag      480

ctggtcatcc ttgtcatttg ggccgtggcc ttctgcagcg cggggcccat cttcgtgctg      540

gtgggcgtgg agcacgagaa cggcacagat ccccgggaca ccaacgagtg ccgcgccacc      600

gagttcgctg tgcgctctgg gctgctcacc gtgatggtat gggtgtcgag cgtcttcttc      660

ttcctgccgg tcttctgcct cactgtgctc tacagtctca tcgggaggaa gctgtggcgg      720

aggcgcggcg acgcggcggt gggctcctcg ctcagggacc agaaccacaa acagacagtg      780

aagatgcttg ctgtggtggt gtttgctttc atcctctgct ggctgccctt ccacgtggga      840

agatatctgt tttccaagtc tttcgagcct ggctctctgg agatcgcgca gatcagtcag      900

tactgcaacc tggtgtcctt tgtcctcttc tacctcagcg ctgccatcaa ccccattctc      960

tacaacatca tgtccaagaa gtaccgggtg gccgtgttca aacttctagg atttgaatcc     1020

ttctcccaga gaaagctttc cactctgaag gatgagagtt cccgggcctg gacaaagtcg     1080

agcatcaata catga                                                      1095


<210>  10
<211>  1107
<212>  DNA
<213>  Synthetic Construct

<400>  10
atggatgcca tgaagagagg gctctgctgt gtgctgctgc tgtgtggagc agtcttcgtt       60

tcgaacagcg agagcgacac gcgtcggaaa cggatgtgga acgcgacgcc cagcgaagag      120

ccggggttca acctcacact ggccgacctg gactgggatg cttcccccgg caacgactcg      180

ctgggcgacg agctgctgca gctcttcccc gcgcgcctct ggcagtaccg gccctggaac      240

ttcggcgacc tcctctgcaa actcttccaa ttcgtcagtg agagctgcac cgtcggggtg      300

gagcacgaga acggcaccga cccttgggac accaacgagt gccgccccac cgagtttgcg      360

gtgcgctctg gcggcggcgg atctagaaaa tgttgtgtcg agtgcccacc gtgcccagca      420

ccacctgtgg caggaccgtc agtcttcctc ttccccccaa aacccaagga ccccctcatg      480

atctcccgga cccctgaggt cacgtgcgtg gtggtggacg tgagccacga agaccccgag      540

gtccagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac aaagccacgg      600

gaggagcagt tcaacagcac gttccgtgtg gtcagcgtcc tcaccgttgt gcaccaggac      660

tggctgaacg gcaaggagta caagtgcaag gtctccaaca aaggcctccc agcccccatc      720

gagaaaacca tctccaaaac caaagggcag ccccgagaac cacaggtgta caccctgccc      780

ccatcccggg aggagatgac caagaaccag gtcagcctga cctgcctggt caaaggcttc      840

taccccagcg acatcgccgt ggagtgggag agcaatgggc agccggagaa caactacaag      900

accacacctc ccatgctgga ctccgacggc tccttcttcc tctacagcaa gctcaccgtg      960

gacaagagca ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg     1020

cacaaccact acacgcagaa gagcctctcc ctgtctccgg gtaaatgata gcggccgcgg     1080

atcccccgac ctcgacctct ggctaat                                         1107


<210>  11
<211>  355
<212>  PRT
<213>  Synthetic Construct

<400>  11

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Asn Ser Glu Ser Asp Thr Arg Arg Lys Arg Met 
            20                  25                  30          


Trp Asn Ala Thr Pro Ser Glu Glu Pro Gly Phe Asn Leu Thr Leu Ala 
        35                  40                  45              


Asp Leu Asp Trp Asp Ala Ser Pro Gly Asn Asp Ser Leu Gly Asp Glu 
    50                  55                  60                  


Leu Leu Gln Leu Phe Pro Ala Arg Leu Trp Gln Tyr Arg Pro Trp Asn 
65                  70                  75                  80  


Phe Gly Asp Leu Leu Cys Lys Leu Phe Gln Phe Val Ser Glu Ser Cys 
                85                  90                  95      


Thr Val Gly Val Glu His Glu Asn Gly Thr Asp Pro Trp Asp Thr Asn 
            100                 105                 110         


Glu Cys Arg Pro Thr Glu Phe Ala Val Arg Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala 
    130                 135                 140                 


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Pro Leu Met 
145                 150                 155                 160 


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
                165                 170                 175     


Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
            180                 185                 190         


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe 
        195                 200                 205             


Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly 
    210                 215                 220                 


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile 
225                 230                 235                 240 


Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val 
                245                 250                 255     


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
            260                 265                 270         


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
        275                 280                 285             


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
    290                 295                 300                 


Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
305                 310                 315                 320 


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
                325                 330                 335     


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
            340                 345                 350         


Pro Gly Lys 
        355 


<210>  12
<211>  120
<212>  DNA
<213>  Homo Sapien

<400>  12
atgtggaacg cgacgcccag cgaagagccg gggttcaacc tcacactggc cgacctggac       60

tgggatgctt cccccggcaa cgactcgctg ggcgacgagc tgctgcagct cttccccgcg      120


<210>  13
<211>  40
<212>  PRT
<213>  Homo Sapien

<400>  13

Met Trp Asn Ala Thr Pro Ser Glu Glu Pro Gly Phe Asn Leu Thr Leu 
1               5                   10                  15      


Ala Asp Leu Asp Trp Asp Ala Ser Pro Gly Asn Asp Ser Leu Gly Asp 
            20                  25                  30          


Glu Leu Leu Gln Leu Phe Pro Ala 
        35                  40  


<210>  14
<211>  78
<212>  DNA
<213>  Homo Sapien

<400>  14
cgcctctggc agtaccggcc ctggaacttc ggcgacctcc tctgcaaact cttccaattc       60

gtcagtgaga gctgcacc                                                     78


<210>  15
<211>  26
<212>  PRT
<213>  Homo Sapien

<400>  15

Arg Leu Trp Gln Tyr Arg Pro Trp Asn Phe Gly Asp Leu Leu Cys Lys 
1               5                   10                  15      


Leu Phe Gln Phe Val Ser Glu Ser Cys Thr 
            20                  25      


<210>  16
<211>  78
<212>  DNA
<213>  Homo Sapien

<400>  16
gtcggggtgg agcacgagaa cggcaccgac ccttgggaca ccaacgagtg ccgccccacc       60

gagtttgcgg tgcgctct                                                     78


<210>  17
<211>  26
<212>  PRT
<213>  Homo Sapien

<400>  17

Val Gly Val Glu His Glu Asn Gly Thr Asp Pro Trp Asp Thr Asn Glu 
1               5                   10                  15      


Cys Arg Pro Thr Glu Phe Ala Val Arg Ser 
            20                  25      


<210>  18
<211>  1101
<212>  DNA
<213>  Homo Sapien

<400>  18
atgtggaacg cgacgcccag cgaagagccg gggttcaacc tcacactggc cgacctggac       60

tgggatgctt cccccggcaa cgactcgctg ggcgacgagc tgctgcagct cttccccgcg      120

ccgctgctgg cgggcgtcac agccacctgc gtggcactct tcgtggtggg catcgctggc      180

aacctgctca ccatgctggt ggtgtcgcgc ttccgcgagc tgcgcaccac caccaacctc      240

tacctgtcca gcatggcctt ctccgatctg ctcatcttcc tctgcatgcc cctggacctc      300

gttcgcctct ggcagtaccg gccctggaac ttcggcgacc tcctctgcaa actcttccaa      360

ttcgtcagtg agagctgcac ctacgccacg gtgctcacca tcacagcgct gagcgtcgag      420

cgctacttcg ccatctgctt cccactccgg gccaaggtgg tggtcaccaa ggggcgggtg      480

aagctggtca tcttcgtcat ctgggccgtg gccttctgca gcgccgggcc catcttcgtg      540

ctagtcgggg tggagcacga gaacggcacc gacccttggg acaccaacga gtgccgcccc      600

accgagtttg cggtgcgctc tggactgctc acggtcatgg tgtgggtgtc cagcatcttc      660

ttcttccttc ctgtcttctg tctcacggtc ctctacagtc tcatcggcag gaagctgtgg      720

cggaggaggc gcggcgatgc tgtcgtgggt gcctcgctca gggaccagaa ccacaagcaa      780

accgtgaaaa tgctggctgt agtggtgttt gccttcatcc tctgctggct ccccttccac      840

gtagggcgat atttattttc caaatccttt gagcctggct ccttggagat tgctcagatc      900

agccagtact gcaacctcgt gtcctttgtc ctcttctacc tcagtgctgc catcaacccc      960

attctgtaca acatcatgtc caagaagtac cgggtggcag tgttcagact tctgggattc     1020

gaacccttct cccagagaaa gctctccact ctgaaagatg aaagttctcg ggcctggaca     1080

gaatctagta ttaatacatg a                                               1101


<210>  19
<211>  28
<212>  DNA
<213>  Mus Musculus

<400>  19
gcggggtacc atgtggaacg cgacgcca                                          28


<210>  20
<211>  33
<212>  DNA
<213>  Mus Musculus

<400>  20
gcgagtactc gcggggaaca gtggcagcag ttc                                    33


<210>  21
<211>  63
<212>  DNA
<213>  Mus Musculus

<400>  21
gcgaagcttt tccagtttgt cagcgagagc tgcacctacg cccccagcga gaccgtcacc       60

tgc                                                                     63


<210>  22
<211>  77
<212>  DNA
<213>  Mus Musculus

<400>  22
cgaagcttgc agagcaggtc gccgaagttc cagggccgat actgccagag gcgcgcgggg       60

aacagtggca gcagttc                                                      77


<210>  23
<211>  81
<212>  DNA
<213>  Mus Musculus

<400>  23
gcgacggatc cccgggacac caacgagtgc cgcgccaccg agttcgctgt gcgctctccc       60

agcgagaccg tcacctgcaa c                                                 81


<210>  24
<211>  66
<212>  DNA
<213>  Mus Musculus

<400>  24
gcggggatcc gtgccgttct cgtgctccac gcccaccagc acggcgtagg tgcagctctc       60

gctgac                                                                  66


<210>  25
<211>  36
<212>  DNA
<213>  Mus Musculus

<400>  25
gcgagtactt ggcccagcga gaccgtcacc tgcaac                                 36


<210>  26
<211>  34
<212>  DNA
<213>  Mus Musculus

<400>  26
gcgctcgagc agggaagaag tctgttatca tgca                                   34


<210>  27
<211>  20
<212>  DNA
<213>  Mus Musculus

<400>  27
ccaaaaccct catcaagacc                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Mus Musculus

<400>  28
tgtctccacc accgaaactc                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Homo Sapien

<400>  29
tgtctccacc accgaaactc                                                   20


<210>  30
<211>  24
<212>  DNA
<213>  Homo Sapien

<400>  30
tctccagttg aaccaagcag gtca                                              24


<210>  31
<211>  22
<212>  DNA
<213>  Homo Sapien

<400>  31
ggaaagacaa cggacaaatc ac                                                22


<210>  32
<211>  20
<212>  DNA
<213>  Homo Sapien

<400>  32
atccttggcc ctctgagatg                                                   20


<210>  33
<211>  21
<212>  DNA
<213>  Mus Musculus

<400>  33
tgtctgaagc agctatggca a                                                 21


<210>  34
<211>  20
<212>  DNA
<213>  Mus Musculus

<400>  34
tgctgcgaga tttgaagctg                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Mus Musculus

<400>  35
tgatcggtcc ccaaagggat                                                   20


<210>  36
<211>  19
<212>  DNA
<213>  Mus Musculus

<400>  36
ttgctacgac gtgggctac                                                    19


