                         SEQUENCE LISTING

<110>  CSL Limited
 
<120>  ANTI-IL-3R ALPHA ANTIBODIES AND USES THEREOF

<130>  511453

<150>  61/783,289
<151>  2013-04-14

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  377
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Val Leu Leu Trp Leu Thr Leu Leu Leu Ile Ala Leu Pro Cys Leu 
1               5                   10                  15      


Leu Gln Thr Lys Glu Asp Pro Asn Pro Pro Ile Thr Asn Leu Arg Met 
            20                  25                  30          


Lys Ala Lys Ala Gln Gln Leu Thr Trp Asp Leu Asn Arg Asn Val Thr 
        35                  40                  45              


Asp Ile Glu Cys Val Lys Asp Ala Asp Tyr Ser Met Pro Ala Val Asn 
    50                  55                  60                  


Asn Ser Tyr Cys Gln Phe Gly Ala Ile Ser Leu Cys Glu Val Thr Asn 
65                  70                  75                  80  


Tyr Thr Val Arg Val Ala Asn Pro Pro Phe Ser Thr Trp Ile Leu Phe 
                85                  90                  95      


Pro Glu Asn Ser Gly Lys Pro Trp Ala Gly Ala Glu Asn Leu Thr Cys 
            100                 105                 110         


Trp Ile His Asp Val Asp Phe Leu Ser Cys Ser Trp Ala Val Gly Pro 
        115                 120                 125             


Gly Ala Pro Ala Asp Val Gln Tyr Asp Leu Tyr Leu Asn Val Ala Lys 
    130                 135                 140                 


Arg Gln Gln Tyr Glu Cys Leu His Tyr Lys Thr Asp Ala Gln Gly Thr 
145                 150                 155                 160 


Arg Ile Gly Cys Arg Phe Asp Asp Ile Ser Arg Leu Ser Ser Gly Ser 
                165                 170                 175     


Gln Ser Ser His Ile Leu Val Arg Gly Arg Ser Ala Ala Phe Gly Ile 
            180                 185                 190         


Pro Cys Thr Asp Lys Phe Val Val Phe Ser Gln Ile Glu Ile Leu Thr 
        195                 200                 205             


Pro Pro Asn Met Thr Ala Lys Cys Asn Lys Thr His Ser Phe Met His 
    210                 215                 220                 


Trp Lys Met Arg Ser His Phe Asn Arg Lys Phe Arg Tyr Glu Leu Gln 
225                 230                 235                 240 


Ile Gln Lys Arg Met Gln Pro Val Ile Thr Glu Gln Val Arg Asp Arg 
                245                 250                 255     


Thr Ser Phe Gln Leu Leu Asn Pro Gly Thr Tyr Thr Val Gln Ile Arg 
            260                 265                 270         


Ala Arg Glu Arg Val Tyr Glu Phe Leu Ser Ala Trp Ser Thr Pro Gln 
        275                 280                 285             


Arg Phe Glu Cys Asp Gln Glu Glu Gly Ala Asn Thr Arg Ala Trp Arg 
    290                 295                 300                 


Thr Ser Leu Leu Ile Ala Leu Gly Thr Leu Leu Ala Leu Val Cys Val 
305                 310                 315                 320 


Phe Val Ile Cys Arg Arg Tyr Leu Val Met Gln Arg Leu Phe Pro Arg 
                325                 330                 335     


Ile Pro His Met Lys Asp Pro Ile Gly Asp Ser Phe Gln Asn Asp Lys 
            340                 345                 350         


Leu Val Val Trp Glu Ala Gly Lys Ala Gly Leu Glu Glu Cys Leu Val 
        355                 360                 365             


Thr Glu Val Gln Val Val Gln Lys Thr 
    370                 375         


<210>  2
<211>  120
<212>  PRT
<213>  Mus musculus

<400>  2

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met Lys Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Asp Ile Ile Pro Ser Asn Gly Ala Thr Phe Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met His Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ser His Leu Leu Arg Ala Ser Trp Phe Ala Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ala 
        115                 120 


<210>  3
<211>  114
<212>  PRT
<213>  Mus musculus

<400>  3

Asp Phe Val Met Thr Gln Ser Pro Ser Ser Leu Thr Val Thr Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Thr Trp Tyr Leu Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp Tyr Ser Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys Arg 
        


<210>  4
<211>  120
<212>  PRT
<213>  Artificial

<220>
<223>  amino acid sequence of heavy chain variable region of humanized 
       anti- IL-3Ralpha chain antibody CSL362 and modified forms thereof

<400>  4

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met Lys Trp Ala Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Asp Ile Ile Pro Ser Asn Gly Ala Thr Phe Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Ser Thr Thr Tyr 
65                  70                  75                  80  


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser His Leu Leu Arg Ala Ser Trp Phe Ala Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Met Val Thr Val Ser Ser 
        115                 120 


<210>  5
<211>  114
<212>  PRT
<213>  Artificial

<220>
<223>  amino acid sequence of light chain variable region of humanized 
       anti- IL-3Ralpha chain antibody CSL362 and modified forms thereof

<400>  5

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Glu Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Thr Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Pro Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp Tyr Ser Tyr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys Arg 
        


<210>  6
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  amino acid sequence of HCDR1 of humanized anti- IL-3Ralpha chain 
       antibody CSL362 and modified forms thereof

<400>  6

Asp Tyr Tyr Met Lys 
1               5   


<210>  7
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  amino acid sequence of HCDR2 of humanized anti- IL-3Ralpha chain 
       antibody CSL362 and modified forms thereof

<400>  7

Asp Ile Ile Pro Ser Asn Gly Ala Thr Phe Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  8
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  mino acid sequence of HCDR3 of a humanized anti- IL-3R alpha 
       chain antibody

<400>  8

Ser His Leu Leu Arg Ala Ser Trp Phe Ala Tyr 
1               5                   10      


<210>  9
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  amino acid sequence of LCDR1 of humanized anti- IL-3Ralpha chain 
       antibody CSL362 and modified forms thereof

<400>  9

Glu Ser Ser Gln Ser Leu Leu Asn Ser Gly Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Thr 
    


<210>  10
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  amino acid sequence of LCDR2 of humanized anti- IL-3Ralpha chain 
       antibody CSL362 and modified forms thereof

<400>  10

Trp Ala Ser Thr Arg Glu Ser 
1               5           


<210>  11
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  amino acid sequence of LCDR3 of humanized anti- IL-3Ralpha chain 
       antibody CSL362 and modified forms thereof

<400>  11

Gln Asn Asp Tyr Ser Tyr Pro Tyr Thr 
1               5                   


<210>  12
<211>  881
<212>  PRT
<213>  Homo sapiens

<400>  12

Trp Glu Arg Ser Leu Ala Gly Ala Glu Glu Thr Ile Pro Leu Gln Thr 
1               5                   10                  15      


Leu Arg Cys Tyr Asn Asp Tyr Thr Ser His Ile Thr Cys Arg Trp Ala 
            20                  25                  30          


Asp Thr Gln Asp Ala Gln Arg Leu Val Asn Val Thr Leu Ile Arg Arg 
        35                  40                  45              


Val Asn Glu Asp Leu Leu Glu Pro Val Ser Cys Asp Leu Ser Asp Asp 
    50                  55                  60                  


Met Pro Trp Ser Ala Cys Pro His Pro Arg Cys Val Pro Arg Arg Cys 
65                  70                  75                  80  


Val Ile Pro Cys Gln Ser Phe Val Val Thr Asp Val Asp Tyr Phe Ser 
                85                  90                  95      


Phe Gln Pro Asp Arg Pro Leu Gly Thr Arg Leu Thr Val Thr Leu Thr 
            100                 105                 110         


Gln His Val Gln Pro Pro Glu Pro Arg Asp Leu Gln Ile Ser Thr Asp 
        115                 120                 125             


Gln Asp His Phe Leu Leu Thr Trp Ser Val Ala Leu Gly Ser Pro Gln 
    130                 135                 140                 


Ser His Trp Leu Ser Pro Gly Asp Leu Glu Phe Glu Val Val Tyr Lys 
145                 150                 155                 160 


Arg Leu Gln Asp Ser Trp Glu Asp Ala Ala Ile Leu Leu Ser Asn Thr 
                165                 170                 175     


Ser Gln Ala Thr Leu Gly Pro Glu His Leu Met Pro Ser Ser Thr Tyr 
            180                 185                 190         


Val Ala Arg Val Arg Thr Arg Leu Ala Pro Gly Ser Arg Leu Ser Gly 
        195                 200                 205             


Arg Pro Ser Lys Trp Ser Pro Glu Val Cys Trp Asp Ser Gln Pro Gly 
    210                 215                 220                 


Asp Glu Ala Gln Pro Gln Asn Leu Glu Cys Phe Phe Asp Gly Ala Ala 
225                 230                 235                 240 


Val Leu Ser Cys Ser Trp Glu Val Arg Lys Glu Val Ala Ser Ser Val 
                245                 250                 255     


Ser Phe Gly Leu Phe Tyr Lys Pro Ser Pro Asp Ala Gly Glu Glu Glu 
            260                 265                 270         


Cys Ser Pro Val Leu Arg Glu Gly Leu Gly Ser Leu His Thr Arg His 
        275                 280                 285             


His Cys Gln Ile Pro Val Pro Asp Pro Ala Thr His Gly Gln Tyr Ile 
    290                 295                 300                 


Val Ser Val Gln Pro Arg Arg Ala Glu Lys His Ile Lys Ser Ser Val 
305                 310                 315                 320 


Asn Ile Gln Met Ala Pro Pro Ser Leu Asn Val Thr Lys Asp Gly Asp 
                325                 330                 335     


Ser Tyr Ser Leu Arg Trp Glu Thr Met Lys Met Arg Tyr Glu His Ile 
            340                 345                 350         


Asp His Thr Phe Glu Ile Gln Tyr Arg Lys Asp Thr Ala Thr Trp Lys 
        355                 360                 365             


Asp Ser Lys Thr Glu Thr Leu Gln Asn Ala His Ser Met Ala Leu Pro 
    370                 375                 380                 


Ala Leu Glu Pro Ser Thr Arg Tyr Trp Ala Arg Val Arg Val Arg Thr 
385                 390                 395                 400 


Ser Arg Thr Gly Tyr Asn Gly Ile Trp Ser Glu Trp Ser Glu Ala Arg 
                405                 410                 415     


Ser Trp Asp Thr Glu Ser Val Leu Pro Met Trp Val Leu Ala Leu Ile 
            420                 425                 430         


Val Ile Phe Leu Thr Ile Ala Val Leu Leu Ala Leu Arg Phe Cys Gly 
        435                 440                 445             


Ile Tyr Gly Tyr Arg Leu Arg Arg Lys Trp Glu Glu Lys Ile Pro Asn 
    450                 455                 460                 


Pro Ser Lys Ser His Leu Phe Gln Asn Gly Ser Ala Glu Leu Trp Pro 
465                 470                 475                 480 


Pro Gly Ser Met Ser Ala Phe Thr Ser Gly Ser Pro Pro His Gln Gly 
                485                 490                 495     


Pro Trp Gly Ser Arg Phe Pro Glu Leu Glu Gly Val Phe Pro Val Gly 
            500                 505                 510         


Phe Gly Asp Ser Glu Val Ser Pro Leu Thr Ile Glu Asp Pro Lys His 
        515                 520                 525             


Val Cys Asp Pro Pro Ser Gly Pro Asp Thr Thr Pro Ala Ala Ser Asp 
    530                 535                 540                 


Leu Pro Thr Glu Gln Pro Pro Ser Pro Gln Pro Gly Pro Pro Ala Ala 
545                 550                 555                 560 


Ser His Thr Pro Glu Lys Gln Ala Ser Ser Phe Asp Phe Asn Gly Pro 
                565                 570                 575     


Tyr Leu Gly Pro Pro His Ser Arg Ser Leu Pro Asp Ile Leu Gly Gln 
            580                 585                 590         


Pro Glu Pro Pro Gln Glu Gly Gly Ser Gln Lys Ser Pro Pro Pro Gly 
        595                 600                 605             


Ser Leu Glu Tyr Leu Cys Leu Pro Ala Gly Gly Gln Val Gln Leu Val 
    610                 615                 620                 


Pro Leu Ala Gln Ala Met Gly Pro Gly Gln Ala Val Glu Val Glu Arg 
625                 630                 635                 640 


Arg Pro Ser Gln Gly Ala Ala Gly Ser Pro Ser Leu Glu Ser Gly Gly 
                645                 650                 655     


Gly Pro Ala Pro Pro Ala Leu Gly Pro Arg Val Gly Gly Gln Asp Gln 
            660                 665                 670         


Lys Asp Ser Pro Val Ala Ile Pro Met Ser Ser Gly Asp Thr Glu Asp 
        675                 680                 685             


Pro Gly Val Ala Ser Gly Tyr Val Ser Ser Ala Asp Leu Val Phe Thr 
    690                 695                 700                 


Pro Asn Ser Gly Ala Ser Ser Val Ser Leu Val Pro Ser Leu Gly Leu 
705                 710                 715                 720 


Pro Ser Asp Gln Thr Pro Ser Leu Cys Pro Gly Leu Ala Ser Gly Pro 
                725                 730                 735     


Pro Gly Ala Pro Gly Pro Val Lys Ser Gly Phe Glu Gly Tyr Val Glu 
            740                 745                 750         


Leu Pro Pro Ile Glu Gly Arg Ser Pro Arg Ser Pro Arg Asn Asn Pro 
        755                 760                 765             


Val Pro Pro Glu Ala Lys Ser Pro Val Leu Asn Pro Gly Glu Arg Pro 
    770                 775                 780                 


Ala Asp Val Ser Pro Thr Ser Pro Gln Pro Glu Gly Leu Leu Val Leu 
785                 790                 795                 800 


Gln Gln Val Gly Asp Tyr Cys Phe Leu Pro Gly Leu Gly Pro Gly Pro 
                805                 810                 815     


Leu Ser Leu Arg Ser Lys Pro Ser Ser Pro Gly Pro Gly Pro Glu Ile 
            820                 825                 830         


Lys Asn Leu Asp Gln Ala Phe Gln Val Lys Lys Pro Pro Gly Gln Ala 
        835                 840                 845             


Val Pro Gln Val Pro Val Ile Gln Leu Phe Lys Ala Leu Lys Gln Gln 
    850                 855                 860                 


Asp Tyr Leu Ser Leu Pro Pro Trp Glu Val Asn Lys Pro Gly Glu Val 
865                 870                 875                 880 


Cys 
    


