                         SEQUENCE LISTING

<110>  INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
       CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
       NICAUD, Jean-Marc
       BEOPOULOS, Athanasios
       PAPANIKOLAOU, Sraphim
 
<120>  LEVURES MUTANTES AYANT UNE PRODUCTION ACCRUE DE LIPIDES ET 
       D'ACIDE CITRIQUE

<130>  XRN/ll-F539_162/PCT

<150>  FR1351893
<151>  2013-03-04

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  520
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  DOMAIN
<222>  (37)..(517)
<223>  rgion prpD

<400>  1

Met Arg Ala Phe Arg Ser Ala Ala Asn Phe Gly Ala Ala Ser Asn Ile 
1               5                   10                  15      


Tyr Arg Lys Ser Phe Thr Pro Ala Ser Ile Ala Ser Asn Arg Phe Val 
            20                  25                  30          


Ser Ala Arg Met Ser Ser Ile Met Thr Asp Asn Ala Arg Pro Asn Thr 
        35                  40                  45              


Asp Lys Val Val Gln Asp Ile Ala Asp Tyr Ile His Asp Tyr Lys Ile 
    50                  55                  60                  


Asp Ser Ser Val Ala Met Glu Thr Ala Arg Leu Cys Phe Leu Asp Thr 
65                  70                  75                  80  


Leu Gly Cys Gly Leu Glu Gly Leu Lys Tyr Gln Gln Cys Ala Asn Ile 
                85                  90                  95      


Val Gly Pro Val Val Pro Gly Thr Ile Val Pro Asn Gly Thr Lys Val 
            100                 105                 110         


Pro Gly Thr Asp Tyr Gln Val Asp Pro Val Arg Gly Ala Phe Asn Ile 
        115                 120                 125             


Gly Thr Ile Ile Arg Trp Leu Asp Phe Asn Asp Cys Trp Leu Ala Ala 
    130                 135                 140                 


Glu Trp Gly His Pro Ser Asp Asn Leu Gly Gly Ile Leu Ala Val Ala 
145                 150                 155                 160 


Asp Trp Gln Thr Arg Ser Ala Lys Ala Gly Leu Glu Gly Lys Val Phe 
                165                 170                 175     


Lys Val Lys Asp Val Leu Glu Gly Met Ile Lys Ala His Glu Ile Gln 
            180                 185                 190         


Gly Gly Leu Ala Ile Glu Asn Ser Phe Asn Arg Val Gly Leu Asp His 
        195                 200                 205             


Val Val Leu Val Lys Ile Ala Ser Thr Ala Val Val Ser Gly Met Leu 
    210                 215                 220                 


Gly Leu Ser Arg Glu Gln Thr Ala Asp Ala Ile Ser Gln Ala Phe Val 
225                 230                 235                 240 


Asp Gly Gln Ser Leu Arg Thr Tyr Arg His Ala Pro Asn Thr Met Ser 
                245                 250                 255     


Arg Lys Ser Trp Ala Ala Gly Asp Ala Thr Ser Arg Ala Val Asn Leu 
            260                 265                 270         


Ala Leu Leu Val Lys Lys Gly Glu Gly Gly Met Pro Ser Ile Leu Thr 
        275                 280                 285             


Ala Lys Thr Trp Gly Phe Tyr Asp Val Leu Phe Gly Gly Lys Glu Phe 
    290                 295                 300                 


Lys Phe Gln Arg Pro Tyr Gly Ser Tyr Val Met Glu Asn Val Leu Phe 
305                 310                 315                 320 


Lys Ile Ser Phe Pro Ala Glu Phe His Ala Gln Thr Ala Cys Glu Ser 
                325                 330                 335     


Ala Met Leu Leu His Glu Glu Leu Lys Lys Leu Gly Lys Thr Ser Asp 
            340                 345                 350         


Asp Ile Ala Ser Ile Lys Ile Arg Thr Gln Glu Ala Ala Met Arg Ile 
        355                 360                 365             


Ile Asp Lys Lys Gly Pro Leu His Asn Tyr Ala Asp Arg Asp His Cys 
    370                 375                 380                 


Ile Gln Tyr Met Val Ala Ile Pro Leu Ile His Gly Arg Leu Thr Ala 
385                 390                 395                 400 


Asp Asp Tyr Thr Asp Glu Ile Ala Ser Asp Pro Arg Ile Asp Ala Leu 
                405                 410                 415     


Arg Glu Lys Met Glu Cys Val Glu Asp Lys Arg Phe Ser Glu Glu Tyr 
            420                 425                 430         


His Ala Pro Asp Lys Arg Tyr Ile Gly Asn Ala Ile Glu Ile Thr Leu 
        435                 440                 445             


Lys Asp Gly Thr Val Leu Asp Glu Ile Glu Val Asn Tyr Pro Ile Gly 
    450                 455                 460                 


His Arg Gln Arg Arg Glu Glu Gly Thr Pro Val Leu Leu Glu Lys Phe 
465                 470                 475                 480 


Ala Arg His Leu Arg Gly Arg Phe Pro Glu Gly Gln Val Glu Lys Ile 
                485                 490                 495     


Leu Ala Ala Ser Asn Gln Asp Ile Val Asn Met Asp Ile Asp Glu Tyr 
            500                 505                 510         


Val Asp Leu Tyr Val Lys Lys Asp 
        515                 520 


<210>  2
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  2
gtcggttggt tggatccg                                                     18


<210>  3
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  3
gcattaccct gttatcccta gcgatcctct caattgggct gg                          42


<210>  4
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  4
gctagggata acagggtaat gccgagatac aggaacgtag tcgg                        44


<210>  5
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  5
cacggggatc cacgactc                                                     18


<210>  6
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  6
gcagtgacac gcgtagtgta gtcgg                                             25


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  7
cacgggaatc cacgactcct cg                                                22


<210>  8
<211>  491
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Squence consensus


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  replace by Leu or Arg

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  replace by Ala, Gln, Asn, Gly or nothing

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  replace by His, Lys, Glu, Val, Leu, Ala or nothing

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  replace by Ala

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  replace by Ser, His, Ile, Asp or Ala

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  replace by  Ile, Thr, Asn, Ser, Val or Ile

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  replace by Gln, Thr, Lys, Ile or Ser

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  replace by Thr, Asp, Ser or Val

<220>
<221>  VARIANT
<222>  (10)..(10)
<223>  replace by Glu, Gln, Lys, Thr, Asp or Ala

<220>
<221>  VARIANT
<222>  (13)..(13)
<223>  replace by Glu, Gln, Asp, Pro or Lys

<220>
<221>  VARIANT
<222>  (14)..(14)
<223>  replace by Pro, Ala, Val or Met

<220>
<221>  VARIANT
<222>  (16)..(16)
<223>  replace by Gln, Glu or Leu

<220>
<221>  VARIANT
<222>  (17)..(17)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (18)..(18)
<223>  replace by Leu

<220>
<221>  VARIANT
<222>  (19)..(19)
<223>  replace by Lys, Arg or Val

<220>
<221>  VARIANT
<222>  (20)..(20)
<223>  replace by Lys

<220>
<221>  VARIANT
<222>  (22)..(22)
<223>  replace by Ser

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  replace by Lys, Ser or Glu

<220>
<221>  VARIANT
<222>  (25)..(25)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (27)..(27)
<223>  replace by Glu, Gln, Asn, His or Lys

<220>
<221>  VARIANT
<222>  (28)..(28)
<223>  replace by Thr, Ala, Lys or His

<220>
<221>  VARIANT
<222>  (29)..(29)
<223>  replace by Pro, Gly Arg or Asp

<220>
<221>  VARIANT
<222>  (30)..(30)
<223>  replace by Leu or Val

<220>
<221>  VARIANT
<222>  (31)..(31)
<223>  replace by Lys, Thr or Asn

<220>
<221>  VARIANT
<222>  (33)..(33)
<223>  replace by Gln, Pro, Thr, Lys or Asn

<220>
<221>  VARIANT
<222>  (34)..(34)
<223>  replace by Leu, Asp or Glu

<220>
<221>  VARIANT
<222>  (36)..(36)
<223>  replace by Leu, Phe or Tyr

<220>
<221>  VARIANT
<222>  (37)..(37)
<223>  replace by Asp or Lys

<220>
<221>  VARIANT
<222>  (40)..(40)
<223>  replace by Lys

<220>
<221>  VARIANT
<222>  (41)..(41)
<223>  replace by Tyr

<220>
<221>  VARIANT
<222>  (43)..(43)
<223>  replace by Leu

<220>
<221>  VARIANT
<222>  (52)..(52)
<223>  replace by Ala

<220>
<221>  VARIANT
<222>  (53)..(53)
<223>  replace by Ala

<220>
<221>  VARIANT
<222>  (55)..(55)
<223>  replace by Arg

<220>
<221>  VARIANT
<222>  (56)..(56)
<223>  replace by Phe or His

<220>
<221>  VARIANT
<222>  (57)..(57)
<223>  replace by Lys, Asp, Asn or Glu

<220>
<221>  VARIANT
<222>  (58)..(58)
<223>  replace by Glu

<220>
<221>  VARIANT
<222>  (59)..(59)
<223>  replace by Ala, Ser, Pro or Val

<220>
<221>  VARIANT
<222>  (60)..(60)
<223>  replace by Gln, Arg, Lys or Ser

<220>
<221>  VARIANT
<222>  (61)..(61)
<223>  replace by Asp, Ala or Gly or Lys

<220>
<221>  VARIANT
<222>  (62)..(62)
<223>  replace by Leu, Val or Met

<220>
<221>  VARIANT
<222>  (63)..(63)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (64)..(64)
<223>  replace by Lys, Thr or Gln

<220>
<221>  VARIANT
<222>  (66)..(66)
<223>  replace by Ile or Thr

<220>
<221>  VARIANT
<222>  (69)..(69)
<223>  replace by Asp

<220>
<221>  VARIANT
<222>  (70)..(70)
<223>  replace by Met

<220>
<221>  VARIANT
<222>  (71)..(71)
<223>  replace by Val

<220>
<221>  VARIANT
<222>  (72)..(72)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (74)..(74)
<223>  replace by Ser

<220>
<221>  VARIANT
<222>  (76)..(76)
<223>  replace by Val

<220>
<221>  VARIANT
<222>  (77)..(77)
<223>  replace by Arg

<220>
<221>  VARIANT
<222>  (78)..(78)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (79)..(79)
<223>  replace by Leu or Ile

<220>
<221>  VARIANT
<222>  (82)..(82)
<223>  replace by Ser, Asn or Lys

<220>
<221>  VARIANT
<222>  (83)..(83)
<223>  replace by His or Ile

<220>
<221>  VARIANT
<222>  (84)..(84)
<223>  replace by Val, Lys or Arg

<220>
<221>  VARIANT
<222>  (85)..(85)
<223>  replace by Met or Leu

<220>
<221>  VARIANT
<222>  (86)..(86)
<223>  replace by Gly

<220>
<221>  VARIANT
<222>  (88)..(88)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (89)..(89)
<223>  replace by Lys, Glu or Ser

<220>
<221>  VARIANT
<222>  (93)..(93)
<223>  replace by Ala or Gly

<220>
<221>  VARIANT
<222>  (97)..(97)
<223>  replace by Leu

<220>
<221>  VARIANT
<222>  (98)..(98)
<223>  replace by Val

<220>
<221>  VARIANT
<222>  (103)..(103)
<223>  replace by Tyr

<220>
<221>  VARIANT
<222>  (123)..(123)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (126)..(126)
<223>  replace by Gly

<220>
<221>  VARIANT
<222>  (128)..(128)
<223>  replace by His or Tyr

<220>
<221>  VARIANT
<222>  (129)..(129)
<223>  replace by Leu, Met, Glu or Ser

<220>
<221>  VARIANT
<222>  (130)..(130)
<223>  replace by Ser

<220>
<221>  VARIANT
<222>  (132)..(132)
<223>  replace by Leu or Thr

<220>
<221>  VARIANT
<222>  (133)..(133)
<223>  replace by Asn, Ser, Tyr or His

<220>
<221>  VARIANT
<222>  (134)..(134)
<223>  replace by Arg or Gln

<220>
<221>  VARIANT
<222>  (135)..(135)
<223>  replace by Ser

<220>
<221>  VARIANT
<222>  (136)..(136)
<223>  replace by Ser or nothing

<220>
<221>  VARIANT
<222>  (137)..(137)
<223>  replace by Ser, Gly, Glu, Asp or nothing

<220>
<221>  VARIANT
<222>  (139)..(139)
<223>  replace by Lys, Glu, Asp, Arg or Gly

<220>
<221>  VARIANT
<222>  (140)..(140)
<223>  replace by Asn or Asp

<220>
<221>  VARIANT
<222>  (142)..(142)
<223>  replace by Arg

<220>
<221>  VARIANT
<222>  (143)..(143)
<223>  replace by Gln, Val, Phe, Leu or Ile

<220>
<221>  VARIANT
<222>  (145)..(145)
<223>  replace by Leu or Thr

<220>
<221>  VARIANT
<222>  (146)..(146)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (147)..(147)
<223>  replace by Gly or Arg

<220>
<221>  VARIANT
<222>  (148)..(148)
<223>  replace by Glu

<220>
<221>  VARIANT
<222>  (149)..(149)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (151)..(151)
<223>  replace by Thr

<220>
<221>  VARIANT
<222>  (152)..(152)
<223>  replace by Ala

<220>
<221>  VARIANT
<222>  (162)..(162)
<223>  replace by Ile, Val or Cys

<220>
<221>  VARIANT
<222>  (163)..(163)
<223>  replace by Ile or Phe

<220>
<221>  VARIANT
<222>  (165)..(165)
<223>  replace by Leu

<220>
<221>  VARIANT
<222>  (166)..(166)
<223>  replace by Asp

<220>
<221>  VARIANT
<222>  (168)..(168)
<223>  replace by Ala

<220>
<221>  VARIANT
<222>  (169)..(169)
<223>  replace by Tyr

<220>
<221>  VARIANT
<222>  (171)..(171)
<223>  replace by Lys or Ser

<220>
<221>  VARIANT
<222>  (178)..(178)
<223>  replace by Ile or Leu

<220>
<221>  VARIANT
<222>  (182)..(182)
<223>  replace by Val

<220>
<221>  VARIANT
<222>  (184)..(184)
<223>  replace by Thr

<220>
<221>  VARIANT
<222>  (185)..(185)
<223>  replace by Ala

<220>
<221>  VARIANT
<222>  (186)..(186)
<223>  replace by Gly

<220>
<221>  VARIANT
<222>  (189)..(189)
<223>  replace by Ala

<220>
<221>  VARIANT
<222>  (190)..(190)
<223>  replace by Lys, Gln or Glu

<220>
<221>  VARIANT
<222>  (193)..(193)
<223>  replace by Asn

<220>
<221>  VARIANT
<222>  (195)..(195)
<223>  replace by Asp, Thr or Asn

<220>
<221>  VARIANT
<222>  (196)..(196)
<223>  replace by Gln, Glu or Ser

<220>
<221>  VARIANT
<222>  (197)..(197)
<223>  replace by Gln, Ala, Ser or Asp

<220>
<221>  VARIANT
<222>  (200)..(200)
<223>  replace by Ile, Val or Thr

<220>
<221>  VARIANT
<222>  (201)..(201)
<223>  replace by Glu or Ala

<220>
<221>  VARIANT
<222>  (203)..(203)
<223>  replace by Leu or Val

<220>
<221>  VARIANT
<222>  (205)..(205)
<223>  replace by His

<220>
<221>  VARIANT
<222>  (208)..(208)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (212)..(212)
<223>  replace by Ala

<220>
<221>  VARIANT
<222>  (219)..(219)
<223>  replace by Ser

<220>
<221>  VARIANT
<222>  (223)..(223)
<223>  replace by Gly

<220>
<221>  VARIANT
<222>  (234)..(234)
<223>  replace by Val or Cys

<220>
<221>  VARIANT
<222>  (235)..(235)
<223>  replace by Ala or Tyr

<220>
<221>  VARIANT
<222>  (239)..(239)
<223>  replace by Lys

<220>
<221>  VARIANT
<222>  (240)..(240)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (241)..(241)
<223>  replace by Cys

<220>
<221>  VARIANT
<222>  (242)..(242)
<223>  replace by Tyr or Phe

<220>
<221>  VARIANT
<222>  (243)..(243)
<223>  replace by His

<220>
<221>  VARIANT
<222>  (244)..(244)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (246)..(246)
<223>  replace by Asn

<220>
<221>  VARIANT
<222>  (247)..(247)
<223>  replace by Ala

<220>
<221>  VARIANT
<222>  (248)..(248)
<223>  replace by Asn, Asp, His, Ser or Gly

<220>
<221>  VARIANT
<222>  (249)..(249)
<223>  replace by Pro or nothing

<220>
<221>  VARIANT
<222>  (250)..(250)
<223>  replace by Asp

<220>
<221>  VARIANT
<222>  (251)..(251)
<223>  replace by Thr or Leu

<220>
<221>  VARIANT
<222>  (252)..(252)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (255)..(255)
<223>  replace by Val or Ala

<220>
<221>  VARIANT
<222>  (259)..(259)
<223>  replace by Arg or Pro

<220>
<221>  VARIANT
<222>  (260)..(260)
<223>  replace by Asp or Asn

<220>
<221>  VARIANT
<222>  (268)..(268)
<223>  replace by Leu

<220>
<221>  VARIANT
<222>  (269)..(269)
<223>  replace by Lys

<220>
<221>  VARIANT
<222>  (270)..(270)
<223>  replace by Ser

<220>
<221>  VARIANT
<222>  (271)..(271)
<223>  replace by Glu, Arg, Asn or Gln

<220>
<221>  VARIANT
<222>  (272)..(272)
<223>  replace by Pro or Ala

<220>
<221>  VARIANT
<222>  (274)..(274)
<223>  replace by Ser, Tyr, Glu or His

<220>
<221>  VARIANT
<222>  (276)..(276)
<223>  replace by Lys or Asn

<220>
<221>  VARIANT
<222>  (277)..(277)
<223>  replace by Gln

<220>
<221>  VARIANT
<222>  (278)..(278)
<223>  replace by nothing

<220>
<221>  VARIANT
<222>  (279)..(279)
<223>  replace by Asn, Asp, Lys, Thr or nothing

<220>
<221>  VARIANT
<222>  (280)..(280)
<223>  replace by Lys, Gln, Ser, Thr or Gly

<220>
<221>  VARIANT
<222>  (281)..(281)
<223>  replace by Phe

<220>
<221>  VARIANT
<222>  (282)..(282)
<223>  replace by Asp, Glu or Arg

<220>
<221>  VARIANT
<222>  (289)..(289)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (295)..(295)
<223>  replace by Tyr

<220>
<221>  VARIANT
<222>  (305)..(305)
<223>  replace by Val or Ala

<220>
<221>  VARIANT
<222>  (307)..(307)
<223>  replace by Ala, Cys or Gly

<220>
<221>  VARIANT
<222>  (309)..(309)
<223>  replace by Val, Ile or Leu

<220>
<221>  VARIANT
<222>  (310)..(310)
<223>  replace by Lys, Ile or Arg

<220>
<221>  VARIANT
<222>  (311)..(311)
<223>  replace by Ala, Val or His

<220>
<221>  VARIANT
<222>  (312)..(312)
<223>  replace by Tyr, Asn or Arg

<220>
<221>  VARIANT
<222>  (313)..(313)
<223>  replace by Arg, Val, Ala, Lys, Asn, Ser or Gln

<220>
<221>  VARIANT
<222>  (314)..(314)
<223>  replace by Ile, Thr, Lys, Gln, Met or Lys

<220>
<221>  VARIANT
<222>  (316)..(316)
<223>  replace by Ala, Asn or Gln

<220>
<221>  VARIANT
<222>  (317)..(317)
<223>  replace by Gln, Ser, Glu or Arg

<220>
<221>  VARIANT
<222>  (318)..(318)
<223>  replace by Gln, Met, Ser, Leu, Lys or Arg

<220>
<221>  VARIANT
<222>  (319)..(319)
<223>  replace by Asn

<220>
<221>  VARIANT
<222>  (320)..(320)
<223>  replace by Val

<220>
<221>  VARIANT
<222>  (321)..(321)
<223>  replace by Ser, Asn or Glu

<220>
<221>  VARIANT
<222>  (322)..(322)
<223>  replace by Phe, Tyr or Val

<220>
<221>  VARIANT
<222>  (323)..(323)
<223>  replace by Lys, Arg or Gln

<220>
<221>  VARIANT
<222>  (325)..(325)
<223>  replace by Val

<220>
<221>  VARIANT
<222>  (326)..(326)
<223>  replace by Lys

<220>
<221>  VARIANT
<222>  (327)..(327)
<223>  replace by Lys or His

<220>
<221>  VARIANT
<222>  (328)..(328)
<223>  replace by Val

<220>
<221>  VARIANT
<222>  (329)..(329)
<223>  replace by Arg or Thr

<220>
<221>  VARIANT
<222>  (334)..(334)
<223>  replace by Asp

<220>
<221>  VARIANT
<222>  (337)..(337)
<223>  replace by Val or Ala

<220>
<221>  VARIANT
<222>  (343)..(343)
<223>  replace by Ser, Thr, Glu or Asp

<220>
<221>  VARIANT
<222>  (347)..(347)
<223>  replace by Tyr

<220>
<221>  VARIANT
<222>  (360)..(360)
<223>  replace by Ile or Thr

<220>
<221>  VARIANT
<222>  (362)..(362)
<223>  replace by Val

<220>
<221>  VARIANT
<222>  (366)..(366)
<223>  replace by Thr or Tyr

<220>
<221>  VARIANT
<222>  (368)..(368)
<223>  replace by Asn

<220>
<221>  VARIANT
<222>  (370)..(370)
<223>  replace by Val

<220>
<221>  VARIANT
<222>  (372)..(372)
<223>  replace by Thr or Glu

<220>
<221>  VARIANT
<222>  (375)..(375)
<223>  replace by Ser, His or Gly

<220>
<221>  VARIANT
<222>  (377)..(377)
<223>  replace by Ala, Leu, Asn or Asp

<220>
<221>  VARIANT
<222>  (378)..(378)
<223>  replace by Val

<220>
<221>  VARIANT
<222>  (380)..(380)
<223>  replace by Arg, Lys, Ser, Leu, Gly or Ala

<220>
<221>  VARIANT
<222>  (381)..(381)
<223>  replace by Asn or Tyr

<220>
<221>  VARIANT
<222>  (383)..(383)
<223>  replace by Glu, Ala or Asp

<220>
<221>  VARIANT
<222>  (386)..(386)
<223>  replace by Asn, Glu or Ile

<220>
<221>  VARIANT
<222>  (389)..(389)
<223>  replace by Ser or Ala

<220>
<221>  VARIANT
<222>  (391)..(391)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (392)..(392)
<223>  replace by Tyr or Gln

<220>
<221>  VARIANT
<222>  (394)..(394)
<223>  replace by Ile, Thr or Glu

<220>
<221>  VARIANT
<222>  (395)..(395)
<223>  replace by Lys, Arg or Val

<220>
<221>  VARIANT
<222>  (396)..(396)
<223>  replace by Glu

<220>
<221>  VARIANT
<222>  (397)..(397)
<223>  replace by Thr or Asp

<220>
<221>  VARIANT
<222>  (398)..(398)
<223>  replace by His, Gln or Thr

<220>
<221>  VARIANT
<222>  (399)..(399)
<223>  replace by Leu or Tyr

<220>
<221>  VARIANT
<222>  (400)..(400)
<223>  replace by Thr

<220>
<221>  VARIANT
<222>  (401)..(401)
<223>  replace by Gln, Leu, Ala or Ile

<220>
<221>  VARIANT
<222>  (402)..(402)
<223>  replace by Asn, Asp or Ala

<220>
<221>  VARIANT
<222>  (404)..(404)
<223>  replace by Asn

<220>
<221>  VARIANT
<222>  (405)..(405)
<223>  replace by Asp, Asn or Glu

<220>
<221>  VARIANT
<222>  (407)..(407)
<223>  replace by Ala, Glu, Ser

<220>
<221>  VARIANT
<222>  (408)..(408)
<223>  replace by Met or Thr

<220>
<221>  VARIANT
<222>  (410)..(411)
<223>  replace by Ser or Ala

<220>
<221>  VARIANT
<222>  (412)..(412)
<223>  replace by Ser or Pro

<220>
<221>  VARIANT
<222>  (414)..(414)
<223>  replace by Gly

<220>
<221>  VARIANT
<222>  (415)..(415)
<223>  replace by Leu

<220>
<221>  VARIANT
<222>  (416)..(416)
<223>  replace by Leu, Lys or Gln

<220>
<221>  VARIANT
<222>  (417)..(417)
<223>  replace by Val

<220>
<221>  VARIANT
<222>  (418)..(418)
<223>  replace by Glu

<220>
<221>  VARIANT
<222>  (419)..(419)
<223>  replace by Phe

<220>
<221>  VARIANT
<222>  (420)..(420)
<223>  replace by Asn or Glu

<220>
<221>  VARIANT
<222>  (422)..(422)
<223>  replace by Asp

<220>
<221>  VARIANT
<222>  (423)..(423)
<223>  replace by Ser

<220>
<221>  VARIANT
<222>  (424)..(424)
<223>  replace by Gln, Lys, Ser, Val or Thr

<220>
<221>  VARIANT
<222>  (425)..(425)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (426)..(426)
<223>  replace by Glu, Asn or Lys

<220>
<221>  VARIANT
<222>  (428)..(428)
<223>  replace by Glu or Val

<220>
<221>  VARIANT
<222>  (429)..(429)
<223>  replace by Phe, Val, Ile or Ala

<220>
<221>  VARIANT
<222>  (430)..(430)
<223>  replace by Ile

<220>
<221>  VARIANT
<222>  (431)..(431)
<223>  replace by Glu or Asp

<220>
<221>  VARIANT
<222>  (434)..(434)
<223>  replace by Val

<220>
<221>  VARIANT
<222>  (437)..(437)
<223>  replace by Lys

<220>
<221>  VARIANT
<222>  (438)..(438)
<223>  replace by Phe, Leu, Thr or Lys

<220>
<221>  VARIANT
<222>  (441)..(441)
<223>  replace by Lys or Ala

<220>
<221>  VARIANT
<222>  (443)..(443)
<223>  replace by Ala

<220>
<221>  VARIANT
<222>  (444)..(444)
<223>  replace by Ile, Val or Glu

<220>
<221>  VARIANT
<222>  (446)..(446)
<223>  replace by Leu or Lys

<220>
<221>  VARIANT
<222>  (448)..(448)
<223>  replace by Met

<220>
<221>  VARIANT
<222>  (449)..(449)
<223>  replace by Asn or Gln

<220>
<221>  VARIANT
<222>  (452)..(452)
<223>  replace by Gln, Lys, Tyr or Glu

<220>
<221>  VARIANT
<222>  (453)..(453)
<223>  replace by Thr, His or Asn

<220>
<221>  VARIANT
<222>  (454)..(454)
<223>  replace by Leu

<220>
<221>  VARIANT
<222>  (455)..(455)
<223>  replace by Val

<220>
<221>  VARIANT
<222>  (456)..(456)
<223>  replace by Asn, Ser or Lys

<220>
<221>  VARIANT
<222>  (457)..(457)
<223>  replace by Glu or Gln

<220>
<221>  VARIANT
<222>  (458)..(458)
<223>  replace by His

<220>
<221>  VARIANT
<222>  (460)..(460)
<223>  replace by Val, Ser, Lys, Ala or Glu

<220>
<221>  VARIANT
<222>  (461)..(461)
<223>  replace by Asn, Gly or Asp

<220>
<221>  VARIANT
<222>  (462)..(462)
<223>  replace by Ser, Asp, Asn, Ala, Glu or Thr

<220>
<221>  VARIANT
<222>  (463)..(463)
<223>  replace by Leu, Ile or nothing

<220>
<221>  VARIANT
<222>  (464)..(464)
<223>  replace by Gly, Glu, Thr, Ser, Asp or nothing

<220>
<221>  VARIANT
<222>  (465)..(465)
<223>  replace by Lys, Arg or Ala

<220>
<221>  VARIANT
<222>  (466)..(466)
<223>  replace by Ala or Ser

<220>
<221>  VARIANT
<222>  (467)..(467)
<223>  replace by Asp, Gln or Lys

<220>
<221>  VARIANT
<222>  (468)..(468)
<223>  replace by Leu, Glu, Thr, Ala, Gln, Asn or Arg

<220>
<221>  VARIANT
<222>  (469)..(469)
<223>  replace by Leu or Val

<220>
<221>  VARIANT
<222>  (470)..(470)
<223>  replace by Met, Tyr or Ile

<220>
<221>  VARIANT
<222>  (471)..(471)
<223>  replace by Lys, Ile, Ser, Glu, Thr or Asn

<220>
<221>  VARIANT
<222>  (472)..(472)
<223>  replace by Val, Lys, Ser, Ala, Met or Lys

<220>
<221>  VARIANT
<222>  (474)..(474)
<223>  replace by Gln, Leu, Thr or nothing

<220>
<221>  VARIANT
<222>  (475)..(475)
<223>  replace by Lys, Asp, Ser, Glu or Gly

<220>
<221>  VARIANT
<222>  (476)..(476)
<223>  replace by Thr, Ser, Glu, Asp or Pro

<220>
<221>  VARIANT
<222>  (477)..(477)
<223>  replace by Asn, Gly or Glu

<220>
<221>  VARIANT
<222>  (478)..(478)
<223>  replace by Phe, Leu or Val

<220>
<221>  VARIANT
<222>  (479)..(479)
<223>  replace by Leu, Glu, Asp or Ile

<220>
<221>  VARIANT
<222>  (480)..(480)
<223>  replace by Ser or Leu

<220>
<221>  VARIANT
<222>  (481)..(481)
<223>  replace by Thr, Leu or Tyr

<220>
<221>  VARIANT
<222>  (482)..(482)
<223>  replace by Gln, Pro, Ser, Thr or Asn

<220>
<221>  VARIANT
<222>  (483)..(483)
<223>  replace by Ile or Val

<220>
<221>  VARIANT
<222>  (485)..(485)
<223>  replace by Lys, Ser, Gln or Asp

<220>
<221>  VARIANT
<222>  (487)..(487)
<223>  replace by Met

<220>
<221>  VARIANT
<222>  (488)..(488)
<223>  replace by Asn or Ser

<220>
<221>  VARIANT
<222>  (489)..(489)
<223>  replace by Met or Thr

<220>
<221>  VARIANT
<222>  (490)..(490)
<223>  replace by Phe

<220>
<221>  VARIANT
<222>  (491)..(491)
<223>  replace by Thr, Trp, Cys or Ile

<400>  8

Ser Ser Thr Ser Ile Met Thr Asp Asn Ala Arg Pro Asn Thr Asp Lys 
1               5                   10                  15      


Val Val Gln Asp Ile Ala Asp Tyr Ile His Asp Tyr Lys Ile Asp Ser 
            20                  25                  30          


Ser Val Ala Met Glu Thr Ala Arg Leu Cys Phe Leu Asp Thr Leu Gly 
        35                  40                  45              


Cys Gly Leu Glu Gly Leu Lys Tyr Gln Gln Cys Ala Asn Ile Val Gly 
    50                  55                  60                  


Pro Val Val Pro Gly Thr Ile Val Pro Asn Gly Thr Lys Val Pro Gly 
65                  70                  75                  80  


Thr Asp Tyr Gln Val Asp Pro Val Arg Gly Ala Phe Asn Ile Gly Thr 
                85                  90                  95      


Ile Ile Arg Trp Leu Asp Phe Asn Asp Cys Trp Leu Ala Ala Glu Trp 
            100                 105                 110         


Gly His Pro Ser Asp Asn Leu Gly Gly Ile Leu Ala Val Ala Asp Trp 
        115                 120                 125             


Gln Thr Arg Ser Ala Lys Thr His Ala Gly Leu Glu Gly Lys Val Phe 
    130                 135                 140                 


Lys Val Lys Asp Val Leu Glu Gly Met Ile Lys Ala His Glu Ile Gln 
145                 150                 155                 160 


Gly Gly Leu Ala Ile Glu Asn Ser Phe Asn Arg Val Gly Leu Asp His 
                165                 170                 175     


Val Val Leu Val Lys Ile Ala Ser Thr Ala Val Val Ser Gly Met Leu 
            180                 185                 190         


Gly Leu Ser Arg Glu Gln Thr Ala Asp Ala Ile Ser Gln Ala Phe Val 
        195                 200                 205             


Asp Gly Gln Ser Leu Arg Thr Tyr Arg His Ala Pro Asn Thr Met Ser 
    210                 215                 220                 


Arg Lys Ser Trp Ala Ala Gly Asp Ala Thr Ser Arg Ala Val Asn Leu 
225                 230                 235                 240 


Ala Leu Leu Val Lys Lys Gly Glu Val Gly Gly Met Pro Ser Ile Leu 
                245                 250                 255     


Thr Ala Lys Thr Trp Gly Phe Tyr Asp Val Leu Phe Gly Gly Lys Glu 
            260                 265                 270         


Phe Lys Phe Gln Arg Arg Ser Pro Tyr Gly Ser Tyr Val Met Glu Asn 
        275                 280                 285             


Val Leu Phe Lys Ile Ser Phe Pro Ala Glu Phe His Ala Gln Thr Ala 
    290                 295                 300                 


Cys Glu Ser Ala Met Leu Leu His Glu Glu Leu Lys Lys Leu Gly Lys 
305                 310                 315                 320 


Thr Ser Asp Asp Ile Ala Ser Ile Lys Ile Arg Thr Gln Glu Ala Ala 
                325                 330                 335     


Met Arg Ile Ile Asp Lys Lys Gly Pro Leu His Asn Tyr Ala Asp Arg 
            340                 345                 350         


Asp His Cys Ile Gln Tyr Met Val Ala Ile Pro Leu Ile His Gly Arg 
        355                 360                 365             


Leu Thr Ala Asp Asp Tyr Thr Asp Glu Ile Ala Ser Asp Pro Arg Ile 
    370                 375                 380                 


Asp Ala Leu Arg Glu Lys Met Glu Cys Val Glu Asp Lys Arg Phe Ser 
385                 390                 395                 400 


Glu Glu Tyr His Ala Pro Asp Lys Arg Tyr Ile Gly Asn Ala Ile Glu 
                405                 410                 415     


Ile Thr Leu Lys Asp Gly Thr Val Leu Asp Glu Ile Glu Val Asn Tyr 
            420                 425                 430         


Pro Ile Gly His Arg Gln Arg Arg Glu Glu Gly Thr Pro Val Leu Leu 
        435                 440                 445             


Glu Lys Phe Ala Arg His Leu Arg Gly Arg Phe Pro Glu Gly Pro Asp 
    450                 455                 460                 


Gln Val Glu Lys Ile Leu Ala Ala Ser Ser Asn Gln Asp Ile Val Asn 
465                 470                 475                 480 


Met Asp Ile Asp Glu Tyr Val Asp Leu Tyr Val 
                485                 490     


<210>  9
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  9
cgcggatccc acaatgtctg ccaacgagaa catctcccga ttcgac                      46


<210>  10
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  10
caccctaggt ctatgatcga gtcttggcct tggaaacgtc                             40


<210>  11
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  11
cacggatccc acaatgtcag cgaaatccat tcacgaggcc gac                         43


<210>  12
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  12
atgcctaggt taaactccga gaggagtgga agcctcagta gaag                        44


<210>  13
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  13
gagagggcga ctggattctc ttctaccac                                         29


<210>  14
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  14
gtggtagaag agaatccagt cgccctctc                                         29


<210>  15
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  15
cttcacccag gttggctcca ccttcaaggg c                                      31


<210>  16
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  16
gcccttgaag gtggagccaa cctgggtgaa g                                      31


<210>  17
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  17
cgcggatccc acaatgttac gac                                               23


<210>  18
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  18
gcgcctaggc tagtcgtaat cccg                                              24


<210>  19
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  19
aacgcggatc ccacaatggc ttcaggatct tcaacg                                 36


<210>  20
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  20
gtccaagctc gggaagctg                                                    19


<210>  21
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  21
ccgttgttag cgatgaggac cttgttgata actgtatgac ctc                         43


<210>  22
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  22
gaggtcatac agttatcaac aaggtcctca tcgctaacaa cg                          42


<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  23
agtatctcat ttccgaggct g                                                 21


<210>  24
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  24
ctggacacca tggctcgtct tgatcccgag tactcctctc tc                          42


<210>  25
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  25
gagagaggag tactcgggat caagacgagc catggtgtcc ag                          42


<210>  26
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce

<400>  26
agctatcgat aatcctaggt cacaacccct tgagcagctc                             40


