                         SEQUENCE LISTING

<110>  Clariant Produkte (Deutschland) GMBH
 
<120>  Improvement of clostridial butanol production by gene 
       overexpression

<130>  170875 n17

<140> EP 13158012.8
<141> 2013-03-06

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  261
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  1

Met Glu Leu Asn Asn Val Ile Leu Glu Lys Glu Gly Lys Val Ala Val 
1               5                   10                  15      


Val Thr Ile Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Ser Asp Thr 
            20                  25                  30          


Leu Lys Glu Met Asp Tyr Val Ile Gly Glu Ile Glu Asn Asp Ser Glu 
        35                  40                  45              


Val Leu Ala Val Ile Leu Thr Gly Ala Gly Glu Lys Ser Phe Val Ala 
    50                  55                  60                  


Gly Ala Asp Ile Ser Glu Met Lys Glu Met Asn Thr Ile Glu Gly Arg 
65                  70                  75                  80  


Lys Phe Gly Ile Leu Gly Asn Lys Val Phe Arg Arg Leu Glu Leu Leu 
                85                  90                  95      


Glu Lys Pro Val Ile Ala Ala Val Asn Gly Phe Ala Leu Gly Gly Gly 
            100                 105                 110         


Cys Glu Ile Ala Met Ser Cys Asp Ile Arg Ile Ala Ser Ser Asn Ala 
        115                 120                 125             


Arg Phe Gly Gln Pro Glu Val Gly Leu Gly Ile Thr Pro Gly Phe Gly 
    130                 135                 140                 


Gly Thr Gln Arg Leu Ser Arg Leu Val Gly Met Gly Met Ala Lys Gln 
145                 150                 155                 160 


Leu Ile Phe Thr Ala Gln Asn Ile Lys Ala Asp Glu Ala Leu Arg Ile 
                165                 170                 175     


Gly Leu Val Asn Lys Val Val Glu Pro Ser Glu Leu Met Asn Thr Ala 
            180                 185                 190         


Lys Glu Ile Ala Asn Lys Ile Val Ser Asn Ala Pro Val Ala Val Lys 
        195                 200                 205             


Leu Ser Lys Gln Ala Ile Asn Arg Gly Met Gln Cys Asp Ile Asp Thr 
    210                 215                 220                 


Ala Leu Ala Phe Glu Ser Glu Ala Phe Gly Glu Cys Phe Ser Thr Glu 
225                 230                 235                 240 


Asp Gln Lys Asp Ala Met Thr Ala Phe Ile Glu Lys Arg Lys Ile Glu 
                245                 250                 255     


Gly Phe Lys Asn Arg 
            260     


<210>  2
<211>  379
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  2

Met Asp Phe Asn Leu Thr Arg Glu Gln Glu Leu Val Arg Gln Met Val 
1               5                   10                  15      


Arg Glu Phe Ala Glu Asn Glu Val Lys Pro Ile Ala Ala Glu Ile Asp 
            20                  25                  30          


Glu Thr Glu Arg Phe Pro Met Glu Asn Val Lys Lys Met Gly Gln Tyr 
        35                  40                  45              


Gly Met Met Gly Ile Pro Phe Ser Lys Glu Tyr Gly Gly Ala Gly Gly 
    50                  55                  60                  


Asp Val Leu Ser Tyr Ile Ile Ala Val Glu Glu Leu Ser Lys Val Cys 
65                  70                  75                  80  


Gly Thr Thr Gly Val Ile Leu Ser Ala His Thr Ser Leu Cys Ala Ser 
                85                  90                  95      


Leu Ile Asn Glu His Gly Thr Glu Glu Gln Lys Gln Lys Tyr Leu Val 
            100                 105                 110         


Pro Leu Ala Lys Gly Glu Lys Ile Gly Ala Tyr Gly Leu Thr Glu Pro 
        115                 120                 125             


Asn Ala Gly Thr Asp Ser Gly Ala Gln Gln Thr Val Ala Val Leu Glu 
    130                 135                 140                 


Gly Asp His Tyr Val Ile Asn Gly Ser Lys Ile Phe Ile Thr Asn Gly 
145                 150                 155                 160 


Gly Val Ala Asp Thr Phe Val Ile Phe Ala Met Thr Asp Arg Thr Lys 
                165                 170                 175     


Gly Thr Lys Gly Ile Ser Ala Phe Ile Ile Glu Lys Gly Phe Lys Gly 
            180                 185                 190         


Phe Ser Ile Gly Lys Val Glu Gln Lys Leu Gly Ile Arg Ala Ser Ser 
        195                 200                 205             


Thr Thr Glu Leu Val Phe Glu Asp Met Ile Val Pro Val Glu Asn Met 
    210                 215                 220                 


Ile Gly Lys Glu Gly Lys Gly Phe Pro Ile Ala Met Lys Thr Leu Asp 
225                 230                 235                 240 


Gly Gly Arg Ile Gly Ile Ala Ala Gln Ala Leu Gly Ile Ala Glu Gly 
                245                 250                 255     


Ala Phe Asn Glu Ala Arg Ala Tyr Met Lys Glu Arg Lys Gln Phe Gly 
            260                 265                 270         


Arg Ser Leu Asp Lys Phe Gln Gly Leu Ala Trp Met Met Ala Asp Met 
        275                 280                 285             


Asp Val Ala Ile Glu Ser Ala Arg Tyr Leu Val Tyr Lys Ala Ala Tyr 
    290                 295                 300                 


Leu Lys Gln Ala Gly Leu Pro Tyr Thr Val Asp Ala Ala Arg Ala Lys 
305                 310                 315                 320 


Leu His Ala Ala Asn Val Ala Met Asp Val Thr Thr Lys Ala Val Gln 
                325                 330                 335     


Leu Phe Gly Gly Tyr Gly Tyr Thr Lys Asp Tyr Pro Val Glu Arg Met 
            340                 345                 350         


Met Arg Asp Ala Lys Ile Thr Glu Ile Tyr Glu Gly Thr Ser Glu Val 
        355                 360                 365             


Gln Lys Leu Val Ile Ser Gly Lys Ile Phe Arg 
    370                 375                 


<210>  3
<211>  282
<212>  PRT
<213>  Clostridium acetobutylicum

<400>  3

Met Lys Lys Val Cys Val Ile Gly Ala Gly Thr Met Gly Ser Gly Ile 
1               5                   10                  15      


Ala Gln Ala Phe Ala Ala Lys Gly Phe Glu Val Val Leu Arg Asp Ile 
            20                  25                  30          


Lys Asp Glu Phe Val Asp Arg Gly Leu Asp Phe Ile Asn Lys Asn Leu 
        35                  40                  45              


Ser Lys Leu Val Lys Lys Gly Lys Ile Glu Glu Ala Thr Lys Val Glu 
    50                  55                  60                  


Ile Leu Thr Arg Ile Ser Gly Thr Val Asp Leu Asn Met Ala Ala Asp 
65                  70                  75                  80  


Cys Asp Leu Val Ile Glu Ala Ala Val Glu Arg Met Asp Ile Lys Lys 
                85                  90                  95      


Gln Ile Phe Ala Asp Leu Asp Asn Ile Cys Lys Pro Glu Thr Ile Leu 
            100                 105                 110         


Ala Ser Asn Thr Ser Ser Leu Ser Ile Thr Glu Val Ala Ser Ala Thr 
        115                 120                 125             


Lys Thr Asn Asp Lys Val Ile Gly Met His Phe Phe Asn Pro Ala Pro 
    130                 135                 140                 


Val Met Lys Leu Val Glu Val Ile Arg Gly Ile Ala Thr Ser Gln Glu 
145                 150                 155                 160 


Thr Phe Asp Ala Val Lys Glu Thr Ser Ile Ala Ile Gly Lys Asp Pro 
                165                 170                 175     


Val Glu Val Ala Glu Ala Pro Gly Phe Val Val Asn Arg Ile Leu Ile 
            180                 185                 190         


Pro Met Ile Asn Glu Ala Val Gly Ile Leu Ala Glu Gly Ile Ala Ser 
        195                 200                 205             


Val Glu Asp Ile Asp Lys Ala Met Lys Leu Gly Ala Asn His Pro Met 
    210                 215                 220                 


Gly Pro Leu Glu Leu Gly Asp Phe Ile Gly Leu Asp Ile Cys Leu Ala 
225                 230                 235                 240 


Ile Met Asp Val Leu Tyr Ser Glu Thr Gly Asp Ser Lys Tyr Arg Pro 
                245                 250                 255     


His Thr Leu Leu Lys Lys Tyr Val Arg Ala Gly Trp Leu Gly Arg Lys 
            260                 265                 270         


Ser Gly Lys Gly Phe Tyr Asp Tyr Ser Lys 
        275                 280         


<210>  4
<211>  135
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  4
tcgacttttt aacaaaatat attgataaaa ataataatag tgggtataat taagttgtta       60

gagaaaacgt ataaattagg gataaactat ggaacttatg aaatagattg aaatggttta      120

tctgttaccc cgtag                                                       135


