                         SEQUENCE LISTING

<110>  Boehringer Ingelheim Vetmedica, Inc.
 
<120>  IMPROVED QUANTIFICATION OF VACCINE COMPOSITIONS

<130>  P10-0154

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  233
<212>  PRT
<213>  Porcine circovirus

<400>  1

Met Thr Tyr Pro Arg Arg Arg Tyr Arg Arg Arg Arg His Arg Pro Arg 
1               5                   10                  15      


Ser His Leu Gly Gln Ile Leu Arg Arg Arg Pro Trp Leu Val His Pro 
            20                  25                  30          


Arg His Arg Tyr Arg Trp Arg Arg Lys Asn Gly Ile Phe Asn Thr Arg 
        35                  40                  45              


Leu Ser Arg Thr Phe Gly Tyr Thr Val Lys Ala Thr Thr Val Thr Thr 
    50                  55                  60                  


Pro Ser Trp Ala Val Asp Met Met Arg Phe Asn Ile Asp Asp Phe Val 
65                  70                  75                  80  


Pro Pro Gly Gly Gly Thr Asn Lys Ile Ser Ile Pro Phe Glu Tyr Tyr 
                85                  90                  95      


Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro Ile Thr 
            100                 105                 110         


Gln Gly Asp Arg Gly Val Gly Ser Thr Ala Val Ile Leu Asp Asp Asn 
        115                 120                 125             


Phe Val Thr Lys Ala Thr Ala Leu Thr Tyr Asp Pro Tyr Val Asn Tyr 
    130                 135                 140                 


Ser Ser Arg His Thr Ile Pro Gln Pro Phe Ser Tyr His Ser Arg Tyr 
145                 150                 155                 160 


Phe Thr Pro Lys Pro Val Leu Asp Ser Thr Ile Asp Tyr Phe Gln Pro 
                165                 170                 175     


Asn Asn Lys Arg Asn Gln Leu Trp Leu Arg Leu Gln Thr Ser Arg Asn 
            180                 185                 190         


Val Asp His Val Gly Leu Gly Thr Ala Phe Glu Asn Ser Lys Tyr Asp 
        195                 200                 205             


Gln Asp Tyr Asn Ile Arg Val Thr Met Tyr Val Gln Phe Arg Glu Phe 
    210                 215                 220                 


Asn Leu Lys Asp Pro Pro Leu Glu Pro 
225                 230             


<210>  2
<211>  233
<212>  PRT
<213>  Porcine circovirus

<400>  2

Met Thr Tyr Pro Arg Arg Arg Tyr Arg Arg Arg Arg His Arg Pro Arg 
1               5                   10                  15      


Ser His Leu Gly Gln Ile Leu Arg Arg Arg Pro Trp Leu Val His Pro 
            20                  25                  30          


Arg His Arg Tyr Arg Trp Arg Arg Lys Asn Gly Ile Phe Asn Thr Arg 
        35                  40                  45              


Leu Ser Arg Thr Phe Gly Tyr Thr Ile Lys Arg Thr Thr Val Arg Thr 
    50                  55                  60                  


Pro Ser Trp Ala Val Asp Met Met Arg Phe Asn Ile Asn Asp Phe Leu 
65                  70                  75                  80  


Pro Pro Gly Gly Gly Ser Asn Pro Arg Ser Val Pro Phe Glu Tyr Tyr 
                85                  90                  95      


Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro Ile Thr 
            100                 105                 110         


Gln Gly Asp Arg Gly Val Gly Ser Ser Ala Val Ile Leu Asp Asp Asn 
        115                 120                 125             


Phe Val Pro Lys Ala Thr Ala Leu Thr Tyr Asp Pro Tyr Val Asn Tyr 
    130                 135                 140                 


Ser Ser Arg His Thr Ile Thr Gln Pro Phe Ser Tyr His Ser Arg Tyr 
145                 150                 155                 160 


Phe Thr Pro Lys Pro Val Leu Asp Gly Thr Ile Asp Tyr Phe Gln Pro 
                165                 170                 175     


Asn Asn Lys Arg Asn Gln Leu Trp Leu Arg Leu Gln Thr Ala Gly Asn 
            180                 185                 190         


Val Asp His Val Gly Leu Gly Thr Ala Phe Glu Asn Ser Ile Tyr Asp 
        195                 200                 205             


Gln Glu Tyr Asn Ile Arg Val Thr Met Tyr Val Gln Phe Arg Glu Phe 
    210                 215                 220                 


Asn Leu Lys Asp Pro Pro Leu Asn Pro 
225                 230             


<210>  3
<211>  234
<212>  PRT
<213>  Porcine circovirus

<400>  3

Met Thr Tyr Pro Arg Arg Arg Phe Arg Arg Arg Arg His Arg Pro Arg 
1               5                   10                  15      


Ser His Leu Gly Gln Ile Leu Arg Arg Arg Pro Trp Leu Val His Pro 
            20                  25                  30          


Arg His Arg Tyr Arg Trp Arg Arg Lys Asn Gly Ile Phe Asn Thr Arg 
        35                  40                  45              


Leu Ser Arg Thr Ile Gly Tyr Thr Val Lys Lys Thr Thr Val Thr Thr 
    50                  55                  60                  


Pro Ser Trp Asn Val Asp Met Met Arg Phe Asn Ile Asn Asp Phe Leu 
65                  70                  75                  80  


Pro Pro Gly Gly Gly Ser Asn Pro Leu Thr Val Pro Phe Glu Tyr Tyr 
                85                  90                  95      


Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro Ile Thr 
            100                 105                 110         


Gln Gly Asp Arg Gly Val Gly Ser Thr Ala Val Ile Leu Asp Asp Asn 
        115                 120                 125             


Phe Val Thr Lys Ala Asn Ala Leu Thr Tyr Asp Pro Tyr Val Asn Tyr 
    130                 135                 140                 


Ser Ser Arg His Thr Ile Thr Gln Pro Phe Ser Tyr His Ser Arg Tyr 
145                 150                 155                 160 


Phe Thr Pro Lys Pro Val Leu Asp Arg Thr Ile Asp Tyr Phe Gln Pro 
                165                 170                 175     


Asn Asn Lys Arg Asn Gln Leu Trp Leu Arg Leu Gln Thr Thr Gly Asn 
            180                 185                 190         


Val Asp His Val Gly Leu Gly Thr Ala Phe Glu Asn Ser Ile Tyr Asp 
        195                 200                 205             


Gln Asp Tyr Asn Ile Arg Ile Thr Met Tyr Val Gln Phe Arg Glu Phe 
    210                 215                 220                 


Asn Leu Lys Asp Pro Pro Leu Asn Pro Lys 
225                 230                 


<210>  4
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  4

Asn Val Asp His Val Gly Leu Gly Thr Ala Phe Glu Asn Ser Lys 
1               5                   10                  15  


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  5

Thr Phe Gly Tyr Thr Val Lys 
1               5           


<210>  6
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  6

Ala Thr Thr Val Thr Thr Pro Ser Trp Ala Val Asp Met Met Arg 
1               5                   10                  15  


<210>  7
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  7

Phe Asn Ile Asp Asp Phe Val Pro Pro Gly Gly Gly Thr Asn Lys Ile 
1               5                   10                  15      


Ser Ile Pro Phe Glu Tyr Tyr Arg 
            20                  


<210>  8
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  8

Ala Thr Ala Leu Thr Tyr Asp Pro Tyr Val Asn Tyr Ser Ser Arg 
1               5                   10                  15  


<210>  9
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  9

His Thr Ile Pro Gln Pro Phe Ser Tyr His Ser Arg 
1               5                   10          


<210>  10
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  10

Tyr Phe Thr Pro Lys Pro Val Leu Asp Ser Thr Ile Asp Tyr Phe Gln 
1               5                   10                  15      


Pro Asn Asn Lys 
            20  


<210>  11
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  11

Val Thr Met Tyr Val Gln Phe Arg 
1               5               


<210>  12
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  12

Met Thr Thr Val Thr Thr Pro Ser Trp Asn Val Asp Met Met Arg 
1               5                   10                  15  


<210>  13
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  13

Phe Asn Ile Asn Asp Phe Leu Pro Pro Gly Gly Gly Ser Asn Pro Leu 
1               5                   10                  15      


Thr Val Pro Phe Glu Tyr Tyr Arg 
            20                  


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  14

Ala Asn Ala Leu Thr Tyr Asp Pro Tyr Val Asn Tyr Ser Ser Arg 
1               5                   10                  15  


<210>  15
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  15

His Thr Ile Thr Gln Pro Phe Ser Tyr His Ser Arg 
1               5                   10          


<210>  16
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  16

Tyr Phe Thr Pro Lys 
1               5   


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  17

Pro Val Leu Asp Arg 
1               5   


<210>  18
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  18

Leu Gln Thr Thr Gly Asn Val Asp His Val Gly Leu Gly Thr Ala Phe 
1               5                   10                  15      


Glu Asn Ser Ile Tyr Asp Gln Asp Tyr Asn Ile Arg 
            20                  25              


<210>  19
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  19

Ile Thr Met Tyr Val Gln Phe Arg 
1               5               


<210>  20
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  20

Glu Phe Asn Leu Lys 
1               5   


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  21

Asp Pro Pro Leu Asn Pro Lys 
1               5           


<210>  22
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  22

Tyr Phe Thr Pro Lys Pro Val Leu Asp Arg 
1               5                   10  


<210>  23
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  23

Glu Phe Asn Leu Lys Asp Pro Pro Leu Asn Pro Lys 
1               5                   10          


<210>  24
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  24

Val Glu Phe Trp Pro Cys Ser Pro Ile Thr Gln Gly Asp Arg 
1               5                   10                  


<210>  25
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  25

Gly Val Gly Ser Thr Ala Val Ile Leu Asp Asp Asn Phe Val Thr Lys 
1               5                   10                  15      


<210>  26
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  26

Ile Ser Ile Pro Phe Glu Tyr Tyr Arg 
1               5                   


<210>  27
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the C 
       terminal amino acid residue

<400>  27

Ser Val Pro Phe Glu Tyr Tyr Arg 
1               5               


<210>  28
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is preferably C13 and N15 isotope labeled at the 
       amino acid residues at sequence positions 7 and 9

<400>  28

Gly Gly Gly Thr Asn Lys Ile Ser Ile Pro Phe Glu Tyr Tyr Arg Ile 
1               5                   10                  15      


Arg Lys Val Lys Val Glu Phe 
            20              


<210>  29
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  this sequence is C13 and N15 isotope labeled at the C terminal 
       amino acid residue

<400>  29

Ser Val Gly Leu Gly Thr Ala Phe Glu Asn Ser Lys 
1               5                   10          


