                         SEQUENCE LISTING

<110>  United Arab Emirates
       El-Agnaf, Omar
 
<120>  ALPHA-SYNUCLEIN ANTIBODIES AND USES THEREOF

<130>  RCD12001

<160>  49    

<170>  PatentIn version 3.5

<210>  1
<211>  426
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(57)
<223>  Leader

<220>
<221>  misc_feature
<222>  (58)..(132)
<223>  FR1

<220>
<221>  misc_feature
<222>  (123)..(162)
<223>  CDR1

<220>
<221>  misc_feature
<222>  (163)..(204)
<223>  FR2

<220>
<221>  misc_feature
<222>  (205)..(255)
<223>  CDR2

<220>
<221>  misc_feature
<222>  (256)..(351)
<223>  FR3

<220>
<221>  misc_feature
<222>  (352)..(393)
<223>  CDR3

<220>
<221>  misc_feature
<222>  (394)..(426)
<223>  FR4

<400>  1
atggactcca ggctcaattt agttttcctt gtccttattt taaaaggtgt ccagtgtgat       60

gtgcagctgg tggagtctgg gggagactta gtgcagcctg gagggtcccg gaaactctcc      120

tgtgcagcct ctggattcac tttcagtagc tttggaatgc actgggttcg tcaggctcca      180

gagaaggggc tggagtgggt cgcatacatt aatagtggca gtagtaccat ctactatgca      240

gacacagtga agggccgatt caccatctcc agagacaatc ccaagaacac cctgttcctg      300

cagatgacca gtctaaggtc tgaggacacg gccatgtatt actgtgcaag gggaaataac      360

cctgggacgg gatattacta ttctatggac tactggggtc agggaacctc agtcaccgtc      420

tcctca                                                                 426


<210>  2
<211>  142
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Asp Ser Arg Leu Asn Leu Val Phe Leu Val Leu Ile Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Asp Val Gln Leu Val Glu Ser Gly Gly Asp Leu Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Arg Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Ser Phe Gly Met His Trp Val Arg Gln Ala Pro Glu Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Tyr Ile Asn Ser Gly Ser Ser Thr Ile Tyr Tyr Ala 
65                  70                  75                  80  


Asp Thr Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Pro Lys Asn 
                85                  90                  95      


Thr Leu Phe Leu Gln Met Thr Ser Leu Arg Ser Glu Asp Thr Ala Met 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Gly Asn Asn Pro Gly Thr Gly Tyr Tyr Tyr Ser 
        115                 120                 125             


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
    130                 135                 140         


<210>  3
<211>  393
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(60)
<223>  Leader

<220>
<221>  misc_feature
<222>  (61)..(129)
<223>  FR1

<220>
<221>  misc_feature
<222>  (130)..(177)
<223>  CDR1

<220>
<221>  misc_feature
<222>  (178)..(222)
<223>  FR2

<220>
<221>  misc_feature
<222>  (223)..(243)
<223>  CDR2

<220>
<221>  misc_feature
<222>  (244)..(339)
<223>  FR3

<220>
<221>  misc_feature
<222>  (340)..(363)
<223>  CDR3

<220>
<221>  misc_feature
<222>  (364)..(393)
<223>  FR4

<400>  3
atgatgagtc ctgcccagtt cctgtttctg ttagtgctct ggattcagga aaccaacggt       60

gatgttgtga tgacccagac tccactcact ttgtcggtta ccattggaca accagcctct      120

atctcttgca agtcaagtca gagcctctta tatagtaatg gaaaaaccta tttgaattgg      180

ttattacaga ggccaggcca gtctccaaag cgcctaatct atctggtgtc taaactggac      240

tctggagtcc ctgacaggtt cactggcagt ggatcaggaa cagattttac actgaaaatc      300

agcagagtgg aggctgagga tttgggagtt tattactgcg tgcaaggtac acattttccc      360

acgttcggag tggggaccaa gctggaaata aaa                                   393


<210>  4
<211>  131
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Met Ser Pro Ala Gln Phe Leu Phe Leu Leu Val Leu Trp Ile Gln 
1               5                   10                  15      


Glu Thr Asn Gly Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser 
            20                  25                  30          


Val Thr Ile Gly Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser 
        35                  40                  45              


Leu Leu Tyr Ser Asn Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg 
    50                  55                  60                  


Pro Gly Gln Ser Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp 
65                  70                  75                  80  


Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 
                85                  90                  95      


Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr 
            100                 105                 110         


Cys Val Gln Gly Thr His Phe Pro Thr Phe Gly Val Gly Thr Lys Leu 
        115                 120                 125             


Glu Ile Lys 
    130     


<210>  5
<211>  426
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(57)
<223>  Leader

<220>
<221>  misc_feature
<222>  (58)..(132)
<223>  FR1

<220>
<221>  misc_feature
<222>  (133)..(162)
<223>  CDR1

<220>
<221>  misc_feature
<222>  (163)..(204)
<223>  FR2

<220>
<221>  misc_feature
<222>  (205)..(255)
<223>  CDR2

<220>
<221>  misc_feature
<222>  (256)..(351)
<223>  FR3

<220>
<221>  misc_feature
<222>  (352)..(393)
<223>  CDR3

<220>
<221>  misc_feature
<222>  (394)..(426)
<223>  FR4

<400>  5
atggactcca ggctcaattt agttttcctt gtccttattt taaaaggtgt ccagtgtgat       60

gtgcagctgg tggagtctgg gggaggctta gtgcagcctg gagggtcccg gaaactctcc      120

tgtgcagcct ctggattcac tttcagtagc tttggaatgc actgggttcg tcaggctcca      180

gagaaggggc tggagtgggt cgcatacatt agtagtggca gtagtaccat ctactatgca      240

gacacagtga agggccgatt caccatctcc agagacaatc ccaagaacac cctgttcctg      300

caaatgacca gtctaaggtc tgaggacacg gccatgtatt actgtgcaag aggaaataac      360

cctgggacgg gatattacta tgctatggac tactggggtc aaggaacctc agtcaccgtc      420

tcctca                                                                 426


<210>  6
<211>  142
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Asp Ser Arg Leu Asn Leu Val Phe Leu Val Leu Ile Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Arg Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Ser Phe Gly Met His Trp Val Arg Gln Ala Pro Glu Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala 
65                  70                  75                  80  


Asp Thr Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Pro Lys Asn 
                85                  90                  95      


Thr Leu Phe Leu Gln Met Thr Ser Leu Arg Ser Glu Asp Thr Ala Met 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Gly Asn Asn Pro Gly Thr Gly Tyr Tyr Tyr Ala 
        115                 120                 125             


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
    130                 135                 140         


<210>  7
<211>  393
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(60)
<223>  Leader

<220>
<221>  misc_feature
<222>  (61)..(129)
<223>  FR1

<220>
<221>  misc_feature
<222>  (130)..(177)
<223>  CDR1

<220>
<221>  misc_feature
<222>  (178)..(222)
<223>  FR2

<220>
<221>  misc_feature
<222>  (223)..(243)
<223>  CDR2

<220>
<221>  misc_feature
<222>  (244)..(339)
<223>  FR3

<220>
<221>  misc_feature
<222>  (340)..(363)
<223>  CDR3

<220>
<221>  misc_feature
<222>  (364)..(393)
<223>  FR4

<400>  7
atgatgagtc ctgcccagtt cctgtttctg ttagtgctct ggattcagga aaccaacggt       60

gatgttgtga tgacccagac tccactcact ttgtcggtta ccattggaca accagcctct      120

atctcttgca agtcaagtca gagcctctta tatagtaatg gaaaaaccta tttgaattgg      180

ttattacaga ggccaggcca gtctccaaag cgcctaatct atctggtgtc taaactggac      240

tctggagtcc ctgacaggtt cactggcagt ggatcaggaa cagattttac actgaaaatc      300

agcagagtgg aggctgagga tttgggagtt tattactgcg tgcaaggtac acattttccc      360

acgttcggag gggggaccaa gctggaaata aaa                                   393


<210>  8
<211>  131
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Met Ser Pro Ala Gln Phe Leu Phe Leu Leu Val Leu Trp Ile Gln 
1               5                   10                  15      


Glu Thr Asn Gly Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser 
            20                  25                  30          


Val Thr Ile Gly Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser 
        35                  40                  45              


Leu Leu Tyr Ser Asn Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg 
    50                  55                  60                  


Pro Gly Gln Ser Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp 
65                  70                  75                  80  


Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 
                85                  90                  95      


Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr 
            100                 105                 110         


Cys Val Gln Gly Thr His Phe Pro Thr Phe Gly Gly Gly Thr Lys Leu 
        115                 120                 125             


Glu Ile Lys 
    130     


<210>  9
<211>  414
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(57)
<223>  Leader

<220>
<221>  misc_feature
<222>  (58)..(132)
<223>  FR1

<220>
<221>  misc_feature
<222>  (133)..(162)
<223>  CDR1

<220>
<221>  misc_feature
<222>  (163)..(204)
<223>  FR2

<220>
<221>  misc_feature
<222>  (205)..(261)
<223>  CDR2

<220>
<221>  misc_feature
<222>  (262)..(357)
<223>  FR3

<220>
<221>  misc_feature
<222>  (358)..(381)
<223>  CDR3

<220>
<221>  misc_feature
<222>  (382)..(414)
<223>  FR4

<400>  9
atggtgttgg ggcttaagtg ggttttcttt gttgtttttt atcaaggtgt gcattgtgag       60

gtgcagcttg ttgagtctgg tggaggattg gtgcagccta aaggatcatt gaaactctca      120

tgtgccgcct ctggtttcac cttcaatacc tatgccatgc actgggtccg ccaggctcca      180

ggaaagggtt tggaatgggt tgctcgcata agaagtaaaa gtagtaatta tgcaacatat      240

tatgccgact cagtgaaaga cagattcacc atctccagag atgattcaca aagcatgctc      300

tatctgcaaa tgaacaacct gaaaactgag gacacagcca tgtattactg tgtgagaccc      360

cttaagtggt acttcgatgt ctggggcaca gggaccacgg tcaccgtctc ctca            414


<210>  10
<211>  138
<212>  PRT
<213>  Homo sapiens

<400>  10

Met Val Leu Gly Leu Lys Trp Val Phe Phe Val Val Phe Tyr Gln Gly 
1               5                   10                  15      


Val His Cys Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Lys Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Asn Thr Tyr Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Arg Ile Arg Ser Lys Ser Ser Asn Tyr Ala Thr Tyr 
65                  70                  75                  80  


Tyr Ala Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser 
                85                  90                  95      


Gln Ser Met Leu Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr 
            100                 105                 110         


Ala Met Tyr Tyr Cys Val Arg Pro Leu Lys Trp Tyr Phe Asp Val Trp 
        115                 120                 125             


Gly Thr Gly Thr Thr Val Thr Val Ser Ser 
    130                 135             


<210>  11
<211>  378
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(60)
<223>  Leader

<220>
<221>  misc_feature
<222>  (61)..(129)
<223>  FR1

<220>
<221>  misc_feature
<222>  (130)..(159)
<223>  CDR1

<220>
<221>  misc_feature
<222>  (160)..(204)
<223>  FR2

<220>
<221>  misc_feature
<222>  (205)..(225)
<223>  CDR2

<220>
<221>  misc_feature
<222>  (226)..(321)
<223>  FR3

<220>
<221>  misc_feature
<222>  (322)..(348)
<223>  CDR3

<220>
<221>  misc_feature
<222>  (349)..(378)
<223>  FR4

<400>  11
atggattttc aagtgcagat tttcagcttc ctgctaatca gtgcctcagt atccagagga       60

caaattgttc tcacccagtc tccagcaatc ttgtctgcat ctccagggga gaaggtcacc      120

atgacctgca gtgccagctc aactgttaat tacatgcact ggtaccagca gaagtcaggc      180

acctccccca aaatatggat ttatgacaca tccaaactgg cttctggagt ccctgctcgc      240

ttcagtggca gtgggtcttg gacctcttac tctctcacaa tcagcagcat ggaggctgaa      300

gatgctgcca cttattactg ccagcagtgg aatagtaacc cacccacgtt cggtgctggg      360

accaagctgg agctgaaa                                                    378


<210>  12
<211>  126
<212>  PRT
<213>  Homo sapiens

<400>  12

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 
1               5                   10                  15      


Val Ser Arg Gly Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Leu Ser 
            20                  25                  30          


Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Thr 
        35                  40                  45              


Val Asn Tyr Met His Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys 
    50                  55                  60                  


Ile Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ala Arg 
65                  70                  75                  80  


Phe Ser Gly Ser Gly Ser Trp Thr Ser Tyr Ser Leu Thr Ile Ser Ser 
                85                  90                  95      


Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Asn Ser 
            100                 105                 110         


Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
        115                 120                 125     


<210>  13
<211>  30
<212>  DNA
<213>  Homo sapiens

<400>  13
ggattcactt tcagtagctt tggaatgcac                                        30


<210>  14
<211>  51
<212>  DNA
<213>  Homo sapiens

<400>  14
tacattaata gtggcagtag taccatctac tatgcagaca cagtgaaggg c                51


<210>  15
<211>  42
<212>  DNA
<213>  Homo sapiens

<400>  15
ggaaataacc ctgggacggg atattactat tctatggact ac                          42


<210>  16
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  16

Gly Phe Thr Phe Ser Ser Phe Gly Met His 
1               5                   10  


<210>  17
<211>  17
<212>  PRT
<213>  Homo sapiens

<400>  17

Tyr Ile Asn Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Thr Val Lys 
1               5                   10                  15      


Gly 
    


<210>  18
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  18

Gly Asn Asn Pro Gly Thr Gly Tyr Tyr Tyr Ser Met Asp Tyr 
1               5                   10                  


<210>  19
<211>  48
<212>  DNA
<213>  Homo sapiens

<400>  19
aagtcaagtc agagcctctt atatagtaat ggaaaaacct atttgaat                    48


<210>  20
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  20
ctggtgtcta aactggactc t                                                 21


<210>  21
<211>  24
<212>  DNA
<213>  Homo sapiens

<400>  21
gtgcaaggta cacattttcc cacg                                              24


<210>  22
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  22

Lys Ser Ser Gln Ser Leu Leu Tyr Ser Asn Gly Lys Thr Tyr Leu Asn 
1               5                   10                  15      


<210>  23
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  23

Leu Val Ser Lys Leu Asp Ser 
1               5           


<210>  24
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  24

Val Gln Gly Thr His Phe Pro Thr 
1               5               


<210>  25
<211>  30
<212>  DNA
<213>  Homo sapiens

<400>  25
ggattcactt tcagtagctt tggaatgcac                                        30


<210>  26
<211>  51
<212>  DNA
<213>  Homo sapiens

<400>  26
tacattagta gtggcagtag taccatctac tatgcagaca cagtgaaggg c                51


<210>  27
<211>  42
<212>  DNA
<213>  Homo sapiens

<400>  27
ggaaataacc ctgggacggg atattactat gctatggact ac                          42


<210>  28
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  28

Gly Phe Thr Phe Ser Ser Phe Gly Met His 
1               5                   10  


<210>  29
<211>  17
<212>  PRT
<213>  Homo sapiens

<400>  29

Tyr Ile Ser Ser Gly Ser Ser Thr Ile Tyr Tyr Ala Asp Thr Val Lys 
1               5                   10                  15      


Gly 
    


<210>  30
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  30

Gly Asn Asn Pro Gly Thr Gly Tyr Tyr Tyr Ala Met Asp Tyr 
1               5                   10                  


<210>  31
<211>  48
<212>  DNA
<213>  Homo sapiens

<400>  31
aagtcaagtc agagcctctt atatagtaat ggaaaaacct atttgaat                    48


<210>  32
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  32
ctggtgtcta aactggactc t                                                 21


<210>  33
<211>  24
<212>  DNA
<213>  Homo sapiens

<400>  33
gtgcaaggta cacattttcc cacg                                              24


<210>  34
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  34

Lys Ser Ser Gln Ser Leu Leu Tyr Ser Asn Gly Lys Thr Tyr Leu Asn 
1               5                   10                  15      


<210>  35
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  35

Leu Val Ser Lys Leu Asp Ser 
1               5           


<210>  36
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  36

Val Gln Gly Thr His Phe Pro Thr 
1               5               


<210>  37
<211>  30
<212>  DNA
<213>  Homo sapiens

<400>  37
ggtttcacct tcaataccta tgccatgcac                                        30


<210>  38
<211>  57
<212>  DNA
<213>  Homo sapiens

<400>  38
cgcataagaa gtaaaagtag taattatgca acatattatg ccgactcagt gaaagac          57


<210>  39
<211>  24
<212>  DNA
<213>  Homo sapiens

<400>  39
ccccttaagt ggtacttcga tgtc                                              24


<210>  40
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  40

Gly Phe Thr Phe Asn Thr Tyr Ala Met His 
1               5                   10  


<210>  41
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  41

Arg Ile Arg Ser Lys Ser Ser Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser 
1               5                   10                  15      


Val Lys Asp 
            


<210>  42
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  42

Pro Leu Lys Trp Tyr Phe Asp Val 
1               5               


<210>  43
<211>  30
<212>  DNA
<213>  Homo sapiens

<400>  43
agtgccagct caactgttaa ttacatgcac                                        30


<210>  44
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  44
gacacatcca aactggcttc t                                                 21


<210>  45
<211>  27
<212>  DNA
<213>  Homo sapiens

<400>  45
cagcagtgga atagtaaccc acccacg                                           27


<210>  46
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  46

Ser Ala Ser Ser Thr Val Asn Tyr Met His 
1               5                   10  


<210>  47
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  47

Asp Thr Ser Lys Leu Ala Ser 
1               5           


<210>  48
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  48

Gln Gln Trp Asn Ser Asn Pro Pro Thr 
1               5                   


<210>  49
<211>  140
<212>  PRT
<213>  Homo sapiens

<400>  49

Met Asp Val Phe Met Lys Gly Leu Ser Lys Ala Lys Glu Gly Val Val 
1               5                   10                  15      


Ala Ala Ala Glu Lys Thr Lys Gln Gly Val Ala Glu Ala Ala Gly Lys 
            20                  25                  30          


Thr Lys Glu Gly Val Leu Tyr Val Gly Ser Lys Thr Lys Glu Gly Val 
        35                  40                  45              


Val His Gly Val Ala Thr Val Ala Glu Lys Thr Lys Glu Gln Val Thr 
    50                  55                  60                  


Asn Val Gly Gly Ala Val Val Thr Gly Val Thr Ala Val Ala Gln Lys 
65                  70                  75                  80  


Thr Val Glu Gly Ala Gly Ser Ile Ala Ala Ala Thr Gly Phe Val Lys 
                85                  90                  95      


Lys Asp Gln Leu Gly Lys Asn Glu Glu Gly Ala Pro Gln Glu Gly Ile 
            100                 105                 110         


Leu Glu Asp Met Pro Val Asp Pro Asp Asn Glu Ala Tyr Glu Met Pro 
        115                 120                 125             


Ser Glu Glu Gly Tyr Gln Asp Tyr Glu Pro Glu Ala 
    130                 135                 140 


