                         SEQUENCE LISTING

<110>  Universittsklinikum Freiburg
 
<120>  Recombinant bispecific antibody that binds to the CD133 antigen 
       on tumor cells and to the CD3 T cell receptor

<130>  PEP02301UNI

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  1455
<212>  DNA
<213>  artificial construct

<220>
<223>  forward strand

<400>  1
caggtgcagc tgcagcagtc tggggctgag ctggtgaggc ctggggcttc agtgaagctg       60

tcctgcaagg cttcgggcta cacattttct gactttgaaa tgcactgggt gaagcagaca      120

cctgtgcatg gcctggaatg gattggagat attgatcctg gaactggtga tactgcctac      180

aatctgaagt tcaagggcaa ggccacactg actacagaca aatcttccag cacagcctac      240

atggagctcc gcagcctgac atctgaggac tctgccgtct attactgtac cctcggggcc      300

tttgtttact ggggccaagg gactctggtc actgtctctg gtggaggcgg ttcagatatc      360

cagatgaccc agtccccgag ctccctgtcc gcctctgtgg gcgatagggt caccatcacc      420

tgtcgtgcca gtcaggacat ccgtaattat ctgaactggt atcaacagaa accaggaaaa      480

gctccgaaac tactgattta ctatacctcc cgcctggagt ctggagtccc ttctcgcttc      540

tctggttctg gttctgggac ggattacact ctgaccatca gcagtctgca accggaggac      600

ttcgcaactt attactgtca gcaaggtaat actctgccgt ggacgttcgg acagggcacc      660

aaggtggaga tcaaaggtgg aggcggttca ggcggaggtg gctctggcgg tggcggatcg      720

gaggttcagc tggtggagtc tggcggtggc ctggtgcagc cagggggctc actccgtttg      780

tcctgtgcag cctctggcta ctcctttacc ggctacacta tgaactgggt gcgtcaggcc      840

ccaggtaagg gcctggaatg ggttgcactg attaatcctt ataaaggtgt ttccacctat      900

aaccagaaat tcaaggatcg tttcacgata tccgtagata aatccaaaaa cacagcctac      960

ctgcaaatga acagcctgcg tgctgaggac actgccgtct attattgtgc tagaagcgga     1020

tactacggcg atagcgactg gtattttgac gtctggggtc aaggaaccct ggtcaccgtc     1080

tcctcgggtg gaggcggttc agatgtggtg gtgacccaga ccccgctgag cctgccggtg     1140

agctttggcg atcaggtgag cattagctgc cgcagcagcc agagcctggc gaacagctat     1200

ggcaacacct atctgagctg gtatctgcat aaaccgggcc agagcccgca gctgctgatt     1260

tatggcatta gcaaccgctt tagcggcgtg ccggatcgct ttagcggcag cggcagcggc     1320

accgatttta ccctgaaaat tagcaccatt aaaccggaag atctgggcat gtattattgc     1380

ctgcagggca cccatcagcc gtataccttt ggcggcggca ccaaactgga aattaaacgc     1440

gctgatgctg cggcc                                                      1455


<210>  2
<211>  1455
<212>  DNA
<213>  artificial construct

<220>
<223>  reverse strand

<400>  2
gtccacgtcg acgtcgtcag accccgactc gaccactccg gaccccgaag tcacttcgac       60

aggacgttcc gaagcccgat gtgtaaaaga ctgaaacttt acgtgaccca cttcgtctgt      120

ggacacgtac cggaccttac ctaacctcta taactaggac cttgaccact atgacggatg      180

ttagacttca agttcccgtt ccggtgtgac tgatgtctgt ttagaaggtc gtgtcggatg      240

tacctcgagg cgtcggactg tagactcctg agacggcaga taatgacatg ggagccccgg      300

aaacaaatga ccccggttcc ctgagaccag tgacagagac cacctccgcc aagtctatag      360

gtctactggg tcaggggctc gagggacagg cggagacacc cgctatccca gtggtagtgg      420

acagcacggt cagtcctgta ggcattaata gacttgacca tagttgtctt tggtcctttt      480

cgaggctttg atgactaaat gatatggagg gcggacctca gacctcaggg aagagcgaag      540

agaccaagac caagaccctg cctaatgtga gactggtagt cgtcagacgt tggcctcctg      600

aagcgttgaa taatgacagt cgttccatta tgagacggca cctgcaagcc tgtcccgtgg      660

ttccacctct agtttccacc tccgccaagt ccgcctccac cgagaccgcc accgcctagc      720

ctccaagtcg accacctcag accgccaccg gaccacgtcg gtcccccgag tgaggcaaac      780

aggacacgtc ggagaccgat gaggaaatgg ccgatgtgat acttgaccca cgcagtccgg      840

ggtccattcc cggaccttac ccaacgtgac taattaggaa tatttccaca aaggtggata      900

ttggtcttta agttcctagc aaagtgctat aggcatctat ttaggttttt gtgtcggatg      960

gacgtttact tgtcggacgc acgactcctg tgacggcaga taataacacg atcttcgcct     1020

atgatgccgc tatcgctgac cataaaactg cagaccccag ttccttggga ccagtggcag     1080

aggagcccac ctccgccaag tctacaccac cactgggtct ggggcgactc ggacggccac     1140

tcgaaaccgc tagtccactc gtaatcgacg gcgtcgtcgg tctcggaccg cttgtcgata     1200

ccgttgtgga tagactcgac catagacgta tttggcccgg tctcgggcgt cgacgactaa     1260

ataccgtaat cgttggcgaa atcgccgcac ggcctagcga aatcgccgtc gccgtcgccg     1320

tggctaaaat gggactttta atcgtggtaa tttggccttc tagacccgta cataataacg     1380

gacgtcccgt gggtagtcgg catatggaaa ccgccgccgt ggtttgacct ttaatttgcg     1440

cgactacgac gccgg                                                      1455


<210>  3
<211>  485
<212>  PRT
<213>  artificial construct

<220>
<223>  diabody

<400>  3

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Ser Asp Phe 
            20                  25                  30          


Glu Met His Trp Val Lys Gln Thr Pro Val His Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Asp Ile Asp Pro Gly Thr Gly Asp Thr Ala Tyr Asn Leu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Leu Gly Ala Phe Val Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
        115                 120                 125             


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser 
    130                 135                 140                 


Gln Asp Ile Arg Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys 
145                 150                 155                 160 


Ala Pro Lys Leu Leu Ile Tyr Tyr Thr Ser Arg Leu Glu Ser Gly Val 
                165                 170                 175     


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr 
            180                 185                 190         


Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
        195                 200                 205             


Gly Asn Thr Leu Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
    210                 215                 220                 


Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
225                 230                 235                 240 


Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
                245                 250                 255     


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 
            260                 265                 270         


Thr Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        275                 280                 285             


Ala Leu Ile Asn Pro Tyr Lys Gly Val Ser Thr Tyr Asn Gln Lys Phe 
    290                 295                 300                 


Lys Asp Arg Phe Thr Ile Ser Val Asp Lys Ser Lys Asn Thr Ala Tyr 
305                 310                 315                 320 


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                325                 330                 335     


Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp 
            340                 345                 350         


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Asp 
        355                 360                 365             


Val Val Val Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Phe Gly Asp 
    370                 375                 380                 


Gln Val Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Ala Asn Ser Tyr 
385                 390                 395                 400 


Gly Asn Thr Tyr Leu Ser Trp Tyr Leu His Lys Pro Gly Gln Ser Pro 
                405                 410                 415     


Gln Leu Leu Ile Tyr Gly Ile Ser Asn Arg Phe Ser Gly Val Pro Asp 
            420                 425                 430         


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser 
        435                 440                 445             


Thr Ile Lys Pro Glu Asp Leu Gly Met Tyr Tyr Cys Leu Gln Gly Thr 
    450                 455                 460                 


His Gln Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
465                 470                 475                 480 


Ala Asp Ala Ala Ala 
                485 


<210>  4
<211>  24
<212>  DNA
<213>  artificial construct

<220>
<223>  CDR1

<400>  4
ggctacacat tttctgactt tgaa                                              24


<210>  5
<211>  24
<212>  DNA
<213>  artificial construct

<220>
<223>  CDR2		Heavy Chain AC133

<400>  5
attgatcctg gaactggtga tact                                              24


<210>  6
<211>  21
<212>  DNA
<213>  artificial construct

<220>
<223>  CDR3		Heavy Chain AC133

<400>  6
accctcgggg cctttgttta c                                                 21


<210>  7
<211>  18
<212>  DNA
<213>  Artificial construct

<220>
<223>  CDR1

<400>  7
caggacatcc gtaattat                                                     18


<210>  8
<211>  9
<212>  DNA
<213>  artificial construct

<220>
<223>  CDR2

<400>  8
tatacctcc                                                                9


<210>  9
<211>  27
<212>  DNA
<213>  artificial construct

<220>
<223>  CDR3

<400>  9
cagcaaggta atactctgcc gtggacg                                           27


<210>  10
<211>  24
<212>  DNA
<213>  artificial construct

<220>
<223>  CDR1

<400>  10
ggctactcct ttaccggcta cact                                              24


<210>  11
<211>  24
<212>  DNA
<213>  artificial construct

<220>
<223>  CDR2

<400>  11
attaatcctt ataaaggtgt ttcc                                              24


<210>  12
<211>  45
<212>  DNA
<213>  artificial construct

<220>
<223>  CDR3

<400>  12
gctagaagcg gatactacgg cgatagcgac tggtattttg acgtc                       45


<210>  13
<211>  33
<212>  DNA
<213>  artificial construct

<220>
<223>  CDR1

<400>  13
cagagcctgg cgaacagcta tggcaacacc tat                                    33


<210>  14
<211>  9
<212>  DNA
<213>  artificial construct

<220>
<223>  CDR2

<400>  14
ggcattagc                                                                9


<210>  15
<211>  18
<212>  DNA
<213>  artificial construct

<220>
<223>  CDR3

<400>  15
ctgcagggca cccatcag                                                     18


