                         SEQUENCE LISTING

<110>  McNulty, Brian
       Stauffer, Maria
 
<120>  USE OF AXMI184 FOR THE CONTROL OF ROOTWORM INSECTS

<130>  APA136001
<140>  US 61/764,246
<141>  2013-02-13

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  2370
<212>  DNA
<213>  Bacillus thuringiensis

<400>  1
atgaacatga acaagaataa tactaaatta agcgcaagag ctttaccaag ttttattgat       60

tattttaatg gcatttatgg atttgccact ggcatcaaag atattatgaa catgattttt      120

aaaacggata caggtggtga tctaacccta gacgaaattt taaagaatca gcagttacta      180

aatgatattt ctggtaaatt ggatggggtg aatggaagct taaatgatct tatcgcacag      240

ggaaacttaa atacagaatt atctaaggaa atattaaaaa ttgcaaatga acaaaatcaa      300

gttttaaatg atgttaataa caaactcgat gcgataaata cgatgcttca tgtatatcta      360

cctaaaatta cctctatgtt aagtgatgta atgaaaccaa attatgcgct aagtatgcaa      420

atagaatacc taagtagaca attacaagaa atttcagata agctagatat tatcaacgta      480

aatgtactta ttaactctac acttactgaa attacacctg cgtatcaatg gattaaatat      540

gtgaacgaaa aatttgaaga attaactttt gctacagaaa ctactttaaa agtaaaaaat      600

gatagcgctt ctgcagatat tcttgatgag ttaacggagt taactgaact tgcgaaaagt      660

gtaacaaaaa atgatgtgga tggttttgaa ttttacctta atacattcca cgatgtaatg      720

gtaggaaata atttattcgg gcgttcgact ttaaaaactg cttcggaatt aattgctaaa      780

gaaaatgtga aaacaagtgg cagtgaggta ggaaatgttt ataatttctt agttgtatta      840

acagctctac aagcaaaagc ttttcttact ttaacaacat gccgaaaatt attaggccta      900

gcagatattg attatacatc tattatgaat gaacatttaa ataaggaaaa agaggaattt      960

agagtaaaca tccttcctat actttctaat actttttcta atcctaatta tgcaaaagtt     1020

aaaggaagtg atgaagatgc aaagatgatt gtggaagcta aaccaggaca tgcattggtt     1080

gggtttgaaa ttagtaatga ttcaatgaca gtattaaaag tatatgaagc taagctaaaa     1140

caaaattacc aagttgataa ggattcctta tcggaagtca tttatggtga tatggataaa     1200

ttattgtgcc cagatcaatc tgaaaaaatt tattatacaa ataatatagt atttccaaat     1260

gaatatgtaa ttactaaaat tgattttact aagaaaatga aaactttaag atatgaggta     1320

acagctaatt cttatgattc ttctacagga gaaattgact taaataaaaa gaaagtagaa     1380

tcaagtaaag cggagtatag gacgttaagt gctaataatg atggagtata tatgccgtta     1440

ggtgtcatca gtgaaacatt tttgactcca attaatggat ttggcctcca agctgatgaa     1500

aattcaagat taattacttt aacgtgtaaa tcatatttaa gggaactact actagcgaca     1560

gacttaagca ataaagaaac taaattgatt gtcccgccaa atagttttat tagcaatatt     1620

gtagagaatg ggtccataga agagggccac ttagagcctt ggaaagcaaa taataagaac     1680

gcttatgtag atcatacagg cggagtgaat ggtactaaag ctctatatgt tcatgaggat     1740

gggggggttt cacaatttat gggagataaa ttaaaaccga aaactgagta tgtaattcaa     1800

tatactgtta aaggaaaacc ttctattcat ttaaaagatg aaaatactgg atatattctt     1860

tatgaagata caaataatga tttagaagat ttccaaacta taactaaaag gttcacaaca     1920

ggaactgatt taatgagagt gtatttaatt ttaaaaagtc aaagtggtca cgaagcttgg     1980

ggagataact ttacaatttt ggaaattaag cctgcggagg ctttagtaag cccagaattg     2040

attaatccga attcttggat tacaactcaa ggggctagca tttcaggaga taaacttttt     2100

attagcttgg ggacaaatgg gacctttaga caaaatcttt cattaaacag ttattcaact     2160

tatagtataa gctttactgc atcgggacca tttaatgtga cggtaagaaa ttctagggaa     2220

gtattatatg aacgaaacaa ccttatgtct tcaactagtc atatttctgg ggaattcaaa     2280

actgaatcca ataataccgg attatatgta gaactttccc gtcgttctgg tggtgctggt     2340

catatatcat ttgaaaacat ttctattaaa                                      2370


<210>  2
<211>  2370
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic gene encoding AXMI184 (optAxmi184)

<400>  2
atgaacatga acaagaacaa caccaagctc tccgcgcgcg cgctgccctc cttcatcgac       60

tacttcaatg gcatctatgg cttcgccacc ggcatcaagg acatcatgaa catgatcttc      120

aagacagaca ctggaggaga tctcaccttg gatgagatcc tcaagaacca gcagctgctg      180

aatgacatct cagggaagct ggatggcgtc aatggaagcc tcaatgatct cattgctcaa      240

ggaaacctca acacagagct ctccaaggag atcctcaaga tcgccaatga gcagaaccag      300

gtgctgaatg atgtcaacaa caagctggat gccatcaaca ccatgctgca tgtttatctt      360

ccaaagatca cctcaatgct ctctgatgtg atgaagccaa actatgctct ctccatgcaa      420

attgagtacc tctcaaggca gctgcaagag atctccgaca agctggacat catcaatgtc      480

aatgtgctga tcaacagcac cttgacagag atcacgccgg cctaccaatg gatcaaatat      540

gtcaatgaga agtttgagga gctcaccttc gccacagaaa caaccttgaa ggtgaagaat      600

gattcagctt ctgctgacat cctggatgag ctgacagagc tgacagagct ggccaagagc      660

gtcaccaaga atgatgttga tggcttcgag ttctacctca acaccttcca tgatgtgatg      720

gtgggcaaca acctctttgg aagaagcacc ctcaagacag cttcagagct gatcgccaag      780

gagaatgtca agacatctgg atcagaagtt ggaaatgtct acaacttcct ggtggtgctg      840

acggcgctgc aagcaaaggc cttcctcacc ctcaccacct gccggaagct gctgggcctc      900

gccgacatcg actacacctc catcatgaat gagcacctca acaaggagaa ggaggagttc      960

agagtgaaca tcctccccat cctctccaac accttcagca accccaacta cgccaaggtg     1020

aagggctcag atgaagatgc caagatgatt gtggaggcca agcctggcca tgctctggtg     1080

ggcttcgaga tcagcaatga cagcatgacg gtgctgaagg tgtatgaagc aaagctgaag     1140

cagaactacc aggtggacaa ggacagcctc tccgaggtga tctatggaga catggacaag     1200

ctgctctgcc cagatcaatc agagaagatc tactacacca acaacatcgt ctttccaaat     1260

gaatatgtca tcaccaagat cgacttcacc aagaagatga aaaccttgag atatgaagtc     1320

accgccaaca gctatgattc ttcaactgga gagatcgacc tcaacaagaa gaaggtggag     1380

agcagcaagg cagagtacag gacgctctcc gccaacaatg atggcgtcta catgccgctc     1440

ggcgtcatct cagaaacctt cttgacgccc atcaatggct tcggcctcca agctgatgag     1500

aacagcaggc tcatcaccct cacctgcaag agctacctca gggagctgct gctggcaaca     1560

gatctctcca acaaggaaac aaagctgatt gttcctccaa acagcttcat cagcaacatc     1620

gtggagaatg gaagcattga agaaggccac ctagagccat ggaaggccaa caacaagaat     1680

gcatatgtgg accacaccgg cggcgtcaat ggcaccaagg cgctctatgt tcatgaagat     1740

ggaggagttt cacagttcat gggagacaag ctgaagccaa aaacagaata tgtcatccag     1800

tacaccgtca agggcaagcc aagcatccac ctcaaggatg agaacaccgg ctacatcctc     1860

tacgaggaca ccaacaatga tttagaagat ttccaaacca tcaccaagag gttcaccact     1920

ggaacagatc tgatgagggt gtacctcatc ctcaagagcc aaagtggtca tgaagcatgg     1980

ggagacaact tcaccatcct ggagatcaag ccagcagaag ctctggtttc tccagagctc     2040

atcaacccca acagctggat aacaacacaa ggagcttcaa tttctggtga caagctcttc     2100

atctcccttg gaacaaatgg caccttccgc cagaacctct ccctcaacag ctacagcacc     2160

tacagcatca gcttcaccgc ctcaggcccc ttcaatgtca ccgtcaggaa cagcagggag     2220

gtgctgtatg aaaggaacaa cttgatgagc agcaccagcc acatctctgg agagttcaag     2280

acagaaagca acaacaccgg cctctatgtg gagctctcaa gaagaagcgg cggcgccggc     2340

cacatcagct tcgagaacat ctccatcaag                                      2370


<210>  3
<211>  790
<212>  PRT
<213>  Bacillus thuringiensis

<400>  3

Met Asn Met Asn Lys Asn Asn Thr Lys Leu Ser Ala Arg Ala Leu Pro 
1               5                   10                  15      


Ser Phe Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile 
            20                  25                  30          


Lys Asp Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu 
        35                  40                  45              


Thr Leu Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Asp Ile Ser 
    50                  55                  60                  


Gly Lys Leu Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln 
65                  70                  75                  80  


Gly Asn Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn 
                85                  90                  95      


Glu Gln Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile 
            100                 105                 110         


Asn Thr Met Leu His Val Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser 
        115                 120                 125             


Asp Val Met Lys Pro Asn Tyr Ala Leu Ser Met Gln Ile Glu Tyr Leu 
    130                 135                 140                 


Ser Arg Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val 
145                 150                 155                 160 


Asn Val Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln 
                165                 170                 175     


Trp Ile Lys Tyr Val Asn Glu Lys Phe Glu Glu Leu Thr Phe Ala Thr 
            180                 185                 190         


Glu Thr Thr Leu Lys Val Lys Asn Asp Ser Ala Ser Ala Asp Ile Leu 
        195                 200                 205             


Asp Glu Leu Thr Glu Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn 
    210                 215                 220                 


Asp Val Asp Gly Phe Glu Phe Tyr Leu Asn Thr Phe His Asp Val Met 
225                 230                 235                 240 


Val Gly Asn Asn Leu Phe Gly Arg Ser Thr Leu Lys Thr Ala Ser Glu 
                245                 250                 255     


Leu Ile Ala Lys Glu Asn Val Lys Thr Ser Gly Ser Glu Val Gly Asn 
            260                 265                 270         


Val Tyr Asn Phe Leu Val Val Leu Thr Ala Leu Gln Ala Lys Ala Phe 
        275                 280                 285             


Leu Thr Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ala Asp Ile Asp 
    290                 295                 300                 


Tyr Thr Ser Ile Met Asn Glu His Leu Asn Lys Glu Lys Glu Glu Phe 
305                 310                 315                 320 


Arg Val Asn Ile Leu Pro Ile Leu Ser Asn Thr Phe Ser Asn Pro Asn 
                325                 330                 335     


Tyr Ala Lys Val Lys Gly Ser Asp Glu Asp Ala Lys Met Ile Val Glu 
            340                 345                 350         


Ala Lys Pro Gly His Ala Leu Val Gly Phe Glu Ile Ser Asn Asp Ser 
        355                 360                 365             


Met Thr Val Leu Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln 
    370                 375                 380                 


Val Asp Lys Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp Met Asp Lys 
385                 390                 395                 400 


Leu Leu Cys Pro Asp Gln Ser Glu Lys Ile Tyr Tyr Thr Asn Asn Ile 
                405                 410                 415     


Val Phe Pro Asn Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr Lys Lys 
            420                 425                 430         


Met Lys Thr Leu Arg Tyr Glu Val Thr Ala Asn Ser Tyr Asp Ser Ser 
        435                 440                 445             


Thr Gly Glu Ile Asp Leu Asn Lys Lys Lys Val Glu Ser Ser Lys Ala 
    450                 455                 460                 


Glu Tyr Arg Thr Leu Ser Ala Asn Asn Asp Gly Val Tyr Met Pro Leu 
465                 470                 475                 480 


Gly Val Ile Ser Glu Thr Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu 
                485                 490                 495     


Gln Ala Asp Glu Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser Tyr 
            500                 505                 510         


Leu Arg Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu Thr Lys 
        515                 520                 525             


Leu Ile Val Pro Pro Asn Ser Phe Ile Ser Asn Ile Val Glu Asn Gly 
    530                 535                 540                 


Ser Ile Glu Glu Gly His Leu Glu Pro Trp Lys Ala Asn Asn Lys Asn 
545                 550                 555                 560 


Ala Tyr Val Asp His Thr Gly Gly Val Asn Gly Thr Lys Ala Leu Tyr 
                565                 570                 575     


Val His Glu Asp Gly Gly Val Ser Gln Phe Met Gly Asp Lys Leu Lys 
            580                 585                 590         


Pro Lys Thr Glu Tyr Val Ile Gln Tyr Thr Val Lys Gly Lys Pro Ser 
        595                 600                 605             


Ile His Leu Lys Asp Glu Asn Thr Gly Tyr Ile Leu Tyr Glu Asp Thr 
    610                 615                 620                 


Asn Asn Asp Leu Glu Asp Phe Gln Thr Ile Thr Lys Arg Phe Thr Thr 
625                 630                 635                 640 


Gly Thr Asp Leu Met Arg Val Tyr Leu Ile Leu Lys Ser Gln Ser Gly 
                645                 650                 655     


His Glu Ala Trp Gly Asp Asn Phe Thr Ile Leu Glu Ile Lys Pro Ala 
            660                 665                 670         


Glu Ala Leu Val Ser Pro Glu Leu Ile Asn Pro Asn Ser Trp Ile Thr 
        675                 680                 685             


Thr Gln Gly Ala Ser Ile Ser Gly Asp Lys Leu Phe Ile Ser Leu Gly 
    690                 695                 700                 


Thr Asn Gly Thr Phe Arg Gln Asn Leu Ser Leu Asn Ser Tyr Ser Thr 
705                 710                 715                 720 


Tyr Ser Ile Ser Phe Thr Ala Ser Gly Pro Phe Asn Val Thr Val Arg 
                725                 730                 735     


Asn Ser Arg Glu Val Leu Tyr Glu Arg Asn Asn Leu Met Ser Ser Thr 
            740                 745                 750         


Ser His Ile Ser Gly Glu Phe Lys Thr Glu Ser Asn Asn Thr Gly Leu 
        755                 760                 765             


Tyr Val Glu Leu Ser Arg Arg Ser Gly Gly Ala Gly His Ile Ser Phe 
    770                 775                 780                 


Glu Asn Ile Ser Ile Lys 
785                 790 


<210>  4
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  targeting peptide

<400>  4

Lys Asp Glu Leu 
1               


