                         SEQUENCE LISTING

<110>  Yissum Research Development Company of the Hebrew 
       University of Jerusalem Ltd.
 
<120>  HYBRID NANOPORES AND USES THEREOF FOR DETECTION OF ANALYTES

<130>  2263832

<150>  US 61/763,164
<151>  2013-02-07

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  108
<212>  PRT
<213>  Populus tremuloides

<400>  1

Met Ala Thr Arg Thr Pro Lys Leu Val Lys His Thr Leu Leu Thr Arg 
1               5                   10                  15      


Phe Lys Asp Glu Ile Thr Arg Glu Gln Ile Asp Asn Tyr Ile Asn Asp 
            20                  25                  30          


Tyr Thr Asn Leu Leu Asp Leu Ile Pro Ser Met Lys Ser Phe Asn Trp 
        35                  40                  45              


Gly Thr Asp Leu Gly Met Glu Ser Ala Glu Leu Asn Arg Gly Tyr Thr 
    50                  55                  60                  


His Ala Phe Glu Ser Thr Phe Glu Ser Lys Ser Gly Leu Gln Glu Tyr 
65                  70                  75                  80  


Leu Asp Ser Ala Ala Leu Ala Ala Phe Ala Glu Gly Phe Leu Pro Thr 
                85                  90                  95      


Leu Ser Gln Arg Leu Val Ile Asp Tyr Phe Leu Tyr 
            100                 105             


<210>  2
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  2

Met His His His His His His Ala Thr Arg Thr Pro Lys Leu Val Lys 
1               5                   10                  15      


His Thr Leu Leu Thr Arg Phe Lys Asp Glu Ile Thr Arg Glu Gln Ile 
            20                  25                  30          


Asp Asn Tyr Ile Asn Asp Tyr Thr Asn Leu Leu Asp Leu Ile Pro Ser 
        35                  40                  45              


Met Lys Ser Phe Asn Trp Gly Thr Asp Leu Gly Met Glu Ser Ala Glu 
    50                  55                  60                  


Leu Asn Arg Gly Tyr Thr His Ala Phe Glu Ser Thr Phe Glu Ser Lys 
65                  70                  75                  80  


Ser Gly Leu Gln Glu Tyr Leu Asp Ser Ala Ala Leu Ala Ala Phe Ala 
                85                  90                  95      


Glu Gly Phe Leu Pro Thr Leu Ser Gln Arg Leu Val Ile Asp Tyr Phe 
            100                 105                 110         


Leu Tyr 
        


<210>  3
<211>  103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  3

Met Lys Leu Val Lys His Thr Leu Leu Thr Arg Phe Lys Asp Glu Ile 
1               5                   10                  15      


Thr Arg Glu Gln Ile Asp Asn Tyr Ile Asn Asp Tyr Thr Asn Leu Leu 
            20                  25                  30          


Asp Leu Ile Pro Ser Met Lys Ser Phe Asn Trp Gly Thr Asp Leu Gly 
        35                  40                  45              


Met Glu Ser Ala Glu Leu Asn Arg Gly Tyr Thr His Ala Phe Glu Ser 
    50                  55                  60                  


Thr Phe Glu Ser Lys Ser Gly Leu Gln Glu Tyr Leu Asp Ser Ala Ala 
65                  70                  75                  80  


Leu Ala Ala Phe Ala Glu Gly Phe Leu Pro Thr Leu Ser Gln Arg Leu 
                85                  90                  95      


Val Ile Asp Tyr Phe Leu Tyr 
            100             


<210>  4
<211>  103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  4

Met Lys Leu Val Lys His Thr Leu Leu Thr Arg Phe Lys Asp Glu Ile 
1               5                   10                  15      


Thr Arg Glu Gln Ile Asp Asn Tyr Ile Asn Asp Tyr Thr Asn Leu Leu 
            20                  25                  30          


Asp Leu Ile Pro Ser Cys Lys Ser Phe Asn Trp Gly Thr Asp Leu Gly 
        35                  40                  45              


Met Glu Ser Ala Glu Leu Asn Arg Gly Tyr Thr His Ala Phe Glu Ser 
    50                  55                  60                  


Thr Phe Glu Ser Lys Ser Gly Leu Gln Glu Tyr Leu Asp Ser Ala Ala 
65                  70                  75                  80  


Leu Ala Ala Phe Ala Glu Gly Phe Leu Pro Thr Leu Ser Gln Arg Leu 
                85                  90                  95      


Val Ile Asp Tyr Phe Leu Tyr 
            100             


<210>  5
<211>  103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  5

Met Lys Leu Val Lys His Thr Leu Leu Thr Arg Phe Lys Asp Glu Ile 
1               5                   10                  15      


Thr Arg Glu Gln Ile Asp Asn Tyr Ile Asn Asp Tyr Thr Asn Leu Leu 
            20                  25                  30          


Asp Leu Ile Pro Ser Met Lys Ser Phe Asn Trp Gly Thr Asp Leu Gly 
        35                  40                  45              


Met Glu Ser Ala Glu Leu Asn Arg Gly Tyr Thr His Ala Phe Glu Ser 
    50                  55                  60                  


Thr Phe Glu Ser Lys Ser Gly Leu Gln Glu Tyr Cys Asp Ser Ala Ala 
65                  70                  75                  80  


Leu Ala Ala Phe Ala Glu Gly Phe Leu Pro Thr Leu Ser Gln Arg Leu 
                85                  90                  95      


Val Ile Asp Tyr Phe Leu Tyr 
            100             


<210>  6
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  6

Lys Leu Val Lys His Thr Leu Leu Thr Arg Phe Lys Asp Glu Ile Thr 
1               5                   10                  15      


Arg Glu Gln Ile Asp Asn Tyr Ile Asn Asp Tyr Thr Asn Leu Leu Asp 
            20                  25                  30          


Leu Cys Pro Ser Met Lys Ser Phe Asn Trp Gly Thr Asp Leu Gly Met 
        35                  40                  45              


Glu Ser Ala Glu Leu Asn Arg Gly Tyr Thr His Ala Phe Glu Ser Thr 
    50                  55                  60                  


Phe Glu Ser Lys Ser Gly Leu Gln Glu Tyr Leu Asp Ser Ala Ala Leu 
65                  70                  75                  80  


Ala Ala Phe Ala Glu Gly Phe Leu Pro Thr Leu Ser Gln Arg Leu Val 
                85                  90                  95      


Ile Asp Tyr Phe Leu Tyr 
            100         


<210>  7
<211>  103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  7

Met Lys Leu Val Lys His Thr Leu Leu Thr Arg Phe Lys Asp Glu Ile 
1               5                   10                  15      


Thr Arg Glu Gln Ile Asp Asn Tyr Ile Asn Asp Tyr Thr Asn Leu Leu 
            20                  25                  30          


Asp Leu Ile Pro Ser Met Lys Ser Phe Asn Trp Gly Thr Asp Leu Gly 
        35                  40                  45              


Met Glu Ser Ala Glu Leu Asn Arg Gly Tyr Thr His Ala Phe Glu Ser 
    50                  55                  60                  


Thr Phe Glu Cys Lys Ser Gly Leu Gln Glu Tyr Leu Asp Ser Ala Ala 
65                  70                  75                  80  


Leu Ala Ala Phe Ala Glu Gly Phe Leu Pro Thr Leu Ser Gln Arg Leu 
                85                  90                  95      


Val Ile Asp Tyr Phe Leu Tyr 
            100             


<210>  8
<211>  327
<212>  DNA
<213>  Populus tremuloides

<400>  8
atggcaacca gaactccaaa gcttgtgaag cacacattgt tgactcggtt caaggatgag       60

atcacacgag aacagatcga caactacatt aatgactata ccaatctgct cgatctcatt      120

ccaagcatga agagtttcaa ttggggcacg gatctgggca tggagtctgc ggagctaaac      180

cgaggataca ctcatgcctt tgaatctaca tttgagagca agtctggttt gcaagagtac      240

ctcgattctg ctgctcttgc tgcatttgca gaagggtttt tgcctacttt gtcacagcgt      300

cttgtgatag actactttct ctactaa                                          327


<210>  9
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  9
atgcaccacc accaccacca cgcaaccaga actccaaaac ttgtgaagca cacattgttg       60

actcggttca aggatgagat cacacgagaa cagatcgaca actacattaa tgactatacc      120

aatctgctcg atctcattcc aagcatgaag agtttcaatt ggggcacgga tctgggcatg      180

gagtctgcgg agctaaaccg aggatacact catgcctttg aatctacatt tgagagcaag      240

tctggtttgc aagagtacct cgattctgct gctcttgctg catttgcaga agggtttttg      300

cctactttgt cacagcgtct tgtgatagac tattttctct actaa                      345


<210>  10
<211>  312
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  10
atgaagcttg tgaagcacac attgttgact cggttcaagg atgagatcac acgagaacag       60

atcgacaact acattaatga ctataccaat ctgctcgatc tcattccaag catgaagagt      120

ttcaattggg gcacggatct gggcatggag tctgcggagc taaaccgagg atacactcat      180

gcctttgaat ctacatttga gagcaagtct ggtttgcaag agtacctcga ttctgctgct      240

cttgctgcat ttgcagaagg gtttttgcct actttgtcac agcgtcttgt gatagactat      300

tttctctact aa                                                          312


<210>  11
<211>  312
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  11
atgaagcttg tgaagcacac attgttgact cggttcaagg atgagatcac acgagaacag       60

atcgacaact acattaatga ctataccaat ctgctcgatc tcattccaag ctgcaagagt      120

ttcaattggg gcacggatct gggcatggag tctgcggagc taaaccgagg atacactcat      180

gcctttgaat ctacatttga gagcaagtct ggtttgcaag agtacctcga ttctgctgct      240

cttgctgcat ttgcagaagg gtttttgcct actttgtcac agcgtcttgt gatagactat      300

tttctctact aa                                                          312


<210>  12
<211>  312
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  12
atgaagcttg tgaagcacac attgttgact cggttcaagg atgagatcac acgagaacag       60

atcgacaact acattaatga ctataccaat ctgctcgatc tcattccaag catgaagagt      120

ttcaattggg gcacggatct gggcatggag tctgcggagc taaaccgagg atacactcat      180

gcctttgaat ctacatttga gagcaagtct ggtttgcaag agtactgcga ttctgctgct      240

cttgctgcat ttgcagaagg gtttttgcct actttgtcac agcgtcttgt gatagactat      300

tttctctact aa                                                          312


<210>  13
<211>  312
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  13
atgaagcttg tgaagcacac attgttgact cggttcaagg atgagatcac acgagaacag       60

atcgacaact acattaatga ctataccaat ctgctcgatc tctgcccaag catgaagagt      120

ttcaattggg gcacggatct gggcatggag tctgcggagc taaaccgagg atacactcat      180

gcctttgaat ctacatttga gagcaagtct ggtttgcaag agtacctcga ttctgctgct      240

cttgctgcat ttgcagaagg gtttttgcct actttgtcac agcgtcttgt gatagactat      300

tttctctact aa                                                          312


<210>  14
<211>  312
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  14
atgaagcttg tgaagcacac attgttgact cggttcaagg atgagatcac acgagaacag       60

atcgacaact acattaatga ctataccaat ctgctcgatc tcattccaag catgaagagt      120

ttcaattggg gcacggatct gggcatggag tctgcggagc taaaccgagg atacactcat      180

gcctttgaat ctacatttga gtgcaagtct ggtttgcaag agtacctcga ttctgctgct      240

cttgctgcat ttgcagaagg gtttttgcct actttgtcac agcgtcttgt gatagactat      300

tttctctact aa                                                          312


<210>  15
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  15

Met Arg Lys Leu Pro Asp Ala Ala Thr Arg Thr Pro Lys Leu Val Lys 
1               5                   10                  15      


His Thr Leu Leu Thr Arg Phe Lys Asp Glu Ile Thr Arg Glu Gln Ile 
            20                  25                  30          


Asp Asn Tyr Ile Asn Asp Tyr Thr Asn Leu Leu Asp Leu Ile Pro Ser 
        35                  40                  45              


Met Lys Ser Phe Asn Trp Gly Thr Asp Leu Gly Met Glu Ser Ala Glu 
    50                  55                  60                  


Leu Asn Arg Gly Tyr Thr His Ala Phe Glu Ser Thr Phe Glu Ser Lys 
65                  70                  75                  80  


Ser Gly Leu Gln Glu Tyr Leu Asp Ser Ala Ala Leu Ala Ala Phe Ala 
                85                  90                  95      


Glu Gly Phe Leu Pro Thr Leu Ser Gln Arg Leu Val Ile Asp Tyr Phe 
            100                 105                 110         


Leu Tyr 
        


<210>  16
<211>  353
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  16
atgcgcaaac ttccggatgc ggcaaccaga actccaaagc ttgtgaagca cacattgttg       60

actcggttca aggatgagat cacacgagaa cagatcgaca actacattaa tgactatacc      120

aatctgctcg atctcattcc aagcatgaag agtttcaatt ggggcacgga tctgggcatg      180

gagtctgcgg agctaaaccg aggatacact catgcctttg aatctacatt tgagagcaag      240

tctggtttgc aagagtacct cgattctgct gctcttgctg catttgcaga agggtttttg      300

cctactttgt cacagcgtct tgtgatagac tactttctct actaaacgct cag             353


<210>  17
<211>  78
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  17

Met Arg Lys Leu Pro Asp Ala Ala Thr Arg Thr Pro Lys Leu Val Lys 
1               5                   10                  15      


His Thr Leu Leu Thr Arg Phe Lys Asp Glu Ile Thr Arg Glu Gln Ile 
            20                  25                  30          


Asp Asn Tyr Ile Asn Asp Tyr Thr Asn Leu Leu Asp Leu Ile Pro Ser 
        35                  40                  45              


Cys Lys Ser Phe Asn Trp Gly Thr Asp Leu Gly Met Glu Ser Ala Glu 
    50                  55                  60                  


Leu Asn Arg Gly Tyr Thr His Ala Phe Glu Ser Thr Phe Glu 
65                  70                  75              


<210>  18
<211>  353
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP1 mutant variant

<400>  18
atgcgcaaac ttccggatgc ggcaaccaga actccaaagc ttgtgaagca cacattgttg       60

actcggttca aggatgagat cacacgagaa cagatcgaca actacattaa tgactatacc      120

aatctgctcg atctcattcc aagctgtaag agtttcaatt ggggcacgga tctgggcatg      180

gagtctgcgg agctaaaccg aggatacact catgcctttg aatctacatt tgagagcaag      240

tctggtttgc aagagtacct cgattctgct gctcttgctg catttgcaga agggtttttg      300

cctactttgt cacagcgtct tgtgatagac tactttctct actaaacgct cag             353


