                         SEQUENCE LISTING

<110>  Green Biologics Ltd
 
<120>  Production of butanol

<130>  489.114060/01

<150>  GB1303595.1
<151>  2013-02-28

<150>  GB1302030.0
<151>  2013-02-05

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  674
<212>  PRT
<213>  Clostridium saccharoperbutylacetonicum

<400>  1

Met Phe Arg Arg Lys Phe Asn Lys Val Ile Leu Ser Ile Leu Val Ala 
1               5                   10                  15      


Thr Ile Val Ser Ser Thr Asn Met Phe Met Ser Gly Ser Lys Ala Gln 
            20                  25                  30          


Ala Ala Ile Gly Asn Leu Ser Glu Asn Asp Thr Ile Tyr Gln Ile Met 
        35                  40                  45              


Val Asp Arg Phe Tyr Asp Gly Asp Lys Thr Asn Asn Ala Thr Gly Asp 
    50                  55                  60                  


Ala Phe Arg Asn Thr Glu Asn Leu Glu Asp Asp Phe Arg Tyr Met His 
65                  70                  75                  80  


Gly Gly Asp Trp Gln Gly Val Ile Asp Lys Leu Asp Tyr Ile Lys Gly 
                85                  90                  95      


Met Gly Tyr Ser Ala Ile Trp Ile Ser Pro Val Ala Glu Pro Gln Met 
            100                 105                 110         


Trp Ser Arg Ala Asp Gly Thr Gly Lys Val Trp Pro Thr Ala Tyr His 
        115                 120                 125             


Gly Tyr Asn Val Lys Asp Pro Asn Lys Ala Asn Pro Tyr Phe Gly Thr 
    130                 135                 140                 


Lys Glu Lys Leu Lys Glu Leu Val Asp Lys Ala His Glu Lys Gly Ile 
145                 150                 155                 160 


Lys Val Ile Ile Asp Ile Val Pro Asn His Val Gly Asp Tyr Met Leu 
                165                 170                 175     


Gly Lys Gln Ala Tyr Tyr Asp Ile Lys Gly Phe Glu Pro Ala Ala Pro 
            180                 185                 190         


Phe Asn Asn Pro Asn Trp Tyr His His Asn Gly Asp Ile Asp Trp Ser 
        195                 200                 205             


Arg Glu His Ser Asp Pro Gln Met Leu Asp Asp His Asp Leu Gly Gly 
    210                 215                 220                 


Leu Asp Asp Leu Asn Gln Asp Asn Ser Asp Ala Lys Ala Ala Met Asn 
225                 230                 235                 240 


Asn Ala Ile Lys Ser Trp Phe Asp Tyr Thr Gly Ala Asp Ala Ala Arg 
                245                 250                 255     


Val Asp Ala Ala Lys Cys Met Lys Pro Ser Tyr Ile Asn Glu Leu Gln 
            260                 265                 270         


Lys Tyr Ile Gly Val Asn Thr Phe Gly Glu Asn Phe Asp Met Asn Val 
        275                 280                 285             


Asp Phe Val Lys Lys Trp Val Gly Ser Asp Ala Glu Trp Gly Met Leu 
    290                 295                 300                 


Asp Phe Pro Leu Tyr Gln Ala Ile Asn Asn Asp Phe Ala Ser Gly Gln 
305                 310                 315                 320 


Ser Phe Asp Asp Met Ser Ser Ser Gly Thr Cys Ser Ile Lys Asn Ile 
                325                 330                 335     


Leu Ala Gln Asp Asn Lys Tyr Asn Gly Tyr Ala Asn His Met Val Thr 
            340                 345                 350         


Phe Ile Asp Asn His Asp Arg Asn Arg Phe Leu Thr Val Ala Asn Gly 
        355                 360                 365             


Asn Val Lys Lys Leu Gln Asn Ala Leu Val Phe Met Phe Thr Val Arg 
    370                 375                 380                 


Gly Val Pro Thr Val Phe Gln Gly Thr Glu Gln Asn Lys Gly Asn Ala 
385                 390                 395                 400 


Asn Gly Ala Ser Ile Asn Gly Ile Ala Asp Thr Trp Asn Arg Trp Ser 
                405                 410                 415     


Met Val Lys Lys Asp Tyr Asn Gly Asn Val Ile Thr Asp Tyr Phe Asn 
            420                 425                 430         


Glu Asn Thr Asp Thr Tyr Lys Leu Ile Asn Lys Leu Asn Ser Phe Arg 
        435                 440                 445             


Gln Lys Tyr Glu Ala Leu Arg Glu Gly Thr Gln Arg Glu Met Trp Ser 
    450                 455                 460                 


Ser Pro His Leu Tyr Ala Phe Ser Arg Arg Met Asp Ser Gly Glu Asn 
465                 470                 475                 480 


Val Gly Gln Glu Val Val Asn Val Phe Asn Asn Ser Asp Gly Asp Gln 
                485                 490                 495     


Ser Ala Thr Ile Pro Ile Arg Ala Glu Ser Thr Ile Lys Val Gly Asp 
            500                 505                 510         


Lys Phe Val Asn Leu Phe Asp Val Asn Asp Ser Ile Thr Val Gln Gln 
        515                 520                 525             


Gly Gly Val Thr Gly Lys Gln Ile Ser Val Asn Leu Gly Glu Asn Ser 
    530                 535                 540                 


Gly Lys Ile Tyr Val Val Asn Asn Glu Thr Pro Asn Pro Asp Gln Lys 
545                 550                 555                 560 


Asn Val Gln Tyr Lys Val Ser Tyr Lys Asn Thr Asn Ala Gln Lys Val 
                565                 570                 575     


Thr Leu His Tyr Gly Thr Asn Gly Trp Lys Asn Ile Gln Asp Val Asn 
            580                 585                 590         


Met Thr Lys Asn Ser Asn Gly Glu Phe Glu Ala Thr Ile Thr Val Asn 
        595                 600                 605             


Asn Asn Asp Ile Leu Asn Tyr Cys Ile His Ile Ile Ser Pro Thr Asp 
    610                 615                 620                 


Tyr Trp Asp Asn Asn Gly Gly Gln Asn Trp Asn Val Lys Val Thr Lys 
625                 630                 635                 640 


Ala Glu Asp Tyr Ile Asn Asp Gly Val Lys Ser Asn Leu Lys Ser Val 
                645                 650                 655     


Asn Thr Thr Thr Ser Ala Ala Ile Asp Ser Gly Ile Asp Ser Thr Val 
            660                 665                 670         


Asn Arg 
        


<210>  2
<211>  2025
<212>  DNA
<213>  Clostridium saccharoperbutylacetonicum

<400>  2
atgtttagaa gaaaatttaa caaggtaata ttatctatct tagttgcaac aattgtttca       60

agcactaaca tgtttatgag tggaagcaag gcacaagcgg caattggaaa tctaagtgaa      120

aacgatacta tttatcaaat tatggtagac agattttatg atggagataa aacaaataat      180

gctacaggag atgcatttcg taatacagaa aatcttgaag atgattttag atatatgcac      240

ggcggagatt ggcaaggtgt tattgataag ttagattata ttaagggcat gggatactca      300

gccatttgga tatcaccggt tgcggaacca caaatgtggt ctagagctga tggcacagga      360

aaagtatggc ctacagctta tcatggatat aatgtgaaag atcccaataa ggcaaatcct      420

tattttggaa caaaagaaaa gctaaaggag ttagtagata aagctcacga aaaggggatt      480

aaagtaataa tagatatagt tccaaatcat gttggggatt atatgttagg aaaacaagct      540

tattatgaca tcaaggggtt tgagccggca gcacctttta ataatccaaa ttggtatcat      600

cataatggcg atattgattg gtcaagagaa cactctgatc cccaaatgtt agatgatcat      660

gatttgggcg gtttagatga tttaaatcaa gataattctg atgctaaggc agctatgaat      720

aatgctatta agtcatggtt tgattatact ggagctgatg cagcaagggt tgacgcagca      780

aaatgtatga aaccatctta tattaacgag ttacaaaagt atataggagt taatactttt      840

ggagaaaatt ttgatatgaa tgtagatttt gtgaagaagt gggttggatc cgatgcagaa      900

tggggaatgc tagattttcc attatatcaa gcaataaata atgattttgc atcaggacaa      960

tcttttgatg acatgtcatc atcaggtact tgctctatta aaaatatttt agcacaagac     1020

aataaatata atggttatgc aaatcatatg gtgactttta tagataatca tgatcgtaat     1080

agatttttaa cagtagcaaa tggtaatgta aaaaaacttc aaaatgcact tgttttcatg     1140

tttactgtaa gaggggtacc aacagtattt caaggtacag aacaaaacaa aggtaatgca     1200

aatggagcaa gtataaatgg tattgcagat acatggaatc gttggtcaat ggttaaaaag     1260

gattacaatg gaaatgtaat tacagattat tttaatgaga atacagatac ttataaacta     1320

attaacaaat tgaattcatt taggcaaaaa tatgaagcct taagagaagg tactcaaaga     1380

gaaatgtggt cttcaccaca tttatatgca ttctcaagaa ggatggattc aggagaaaat     1440

gttggacaag aagttgtaaa tgtatttaat aattcagatg gagatcaaag tgcgaccatt     1500

ccaattagag ctgaaagtac tataaaagtt ggagataaat ttgtaaatct ttttgatgta     1560

aatgattcga tcacagttca acaaggaggt gttacaggaa aacaaatatc agtgaattta     1620

ggagaaaata gtgggaagat ttatgttgtt aataatgaaa caccaaatcc agatcaaaag     1680

aacgtacaat ataaagtttc atataagaat actaatgcac aaaaagtaac acttcattat     1740

ggaactaatg gatggaaaaa cattcaagat gtaaatatga ctaagaattc caatggagaa     1800

tttgaagcaa ctattacagt aaataataat gatattctaa attactgtat tcatattatt     1860

tcaccaacag actattggga taataatggt ggacagaatt ggaatgtaaa agtgactaag     1920

gcagaagatt atataaatga tggtgtaaag agtaatttga agagcgttaa tacaactaca     1980

tcagcagcta tagactctgg gattgatagt actgtaaatc gttaa                     2025


<210>  3
<211>  674
<212>  PRT
<213>  Clostridium saccharoperbutylacetonicum

<400>  3

Met Phe Arg Arg Lys Phe Asn Lys Val Ile Leu Ser Ile Leu Val Ala 
1               5                   10                  15      


Thr Ile Val Ser Ser Thr Asn Met Phe Met Ser Gly Ser Lys Ala Gln 
            20                  25                  30          


Ala Ala Ile Gly Asn Leu Ser Glu Asn Asp Thr Ile Tyr Gln Ile Met 
        35                  40                  45              


Val Asp Arg Phe Tyr Asp Gly Asp Lys Thr Asn Asn Ala Thr Gly Asp 
    50                  55                  60                  


Ala Phe Arg Asn Thr Glu Asn Leu Glu Asp Asp Phe Arg Tyr Met His 
65                  70                  75                  80  


Gly Gly Asp Trp Gln Gly Val Ile Asp Lys Leu Asp Tyr Ile Lys Gly 
                85                  90                  95      


Met Gly Tyr Ser Ala Ile Trp Ile Ser Pro Val Ala Glu Pro Gln Met 
            100                 105                 110         


Trp Ser Arg Ala Asp Gly Thr Gly Lys Val Trp Pro Thr Ala Tyr His 
        115                 120                 125             


Gly Tyr Asn Val Lys Asp Pro Asn Lys Ala Asn Pro Tyr Phe Gly Thr 
    130                 135                 140                 


Lys Glu Lys Leu Lys Glu Leu Val Asp Lys Ala His Glu Lys Gly Ile 
145                 150                 155                 160 


Lys Val Ile Ile Asp Ile Val Pro Asn His Val Gly Asp Tyr Met Leu 
                165                 170                 175     


Gly Lys Gln Ala Tyr Tyr Asp Ile Lys Gly Phe Glu Pro Ala Ala Pro 
            180                 185                 190         


Phe Asn Asn Pro Asn Trp Tyr His His Asn Gly Asp Ile Asp Trp Ser 
        195                 200                 205             


Arg Glu His Ser Asp Pro Gln Met Leu Asp Asp His Asp Leu Gly Gly 
    210                 215                 220                 


Leu Asp Asp Leu Asn Gln Asp Asn Ser Asp Ala Lys Ala Ala Met Asn 
225                 230                 235                 240 


Asn Ala Ile Lys Ser Trp Phe Asp Tyr Thr Gly Ala Asp Ala Ala Arg 
                245                 250                 255     


Val Asp Ala Ala Lys Cys Met Lys Pro Ser Tyr Ile Asn Glu Leu Gln 
            260                 265                 270         


Lys Tyr Ile Gly Val Asn Thr Phe Gly Glu Asn Phe Asp Met Asn Val 
        275                 280                 285             


Asp Phe Val Lys Lys Trp Val Gly Ser Asp Ala Glu Trp Gly Met Leu 
    290                 295                 300                 


Asp Phe Pro Leu Tyr Gln Ala Ile Asn Asn Asp Phe Ala Ser Gly Gln 
305                 310                 315                 320 


Ser Phe Asp Asp Met Ser Ser Ser Gly Thr Cys Ser Ile Lys Asn Ile 
                325                 330                 335     


Leu Ala Gln Asp Asn Lys Tyr Asn Gly Tyr Ala Asn His Met Val Thr 
            340                 345                 350         


Phe Ile Asp Asn His Asp Arg Asn Arg Phe Leu Thr Val Ala Asn Gly 
        355                 360                 365             


Asn Val Lys Lys Leu Gln Asn Ala Leu Val Phe Met Phe Thr Val Arg 
    370                 375                 380                 


Gly Val Pro Thr Val Phe Gln Gly Thr Glu Gln Asn Lys Gly Asn Gly 
385                 390                 395                 400 


Asn Gly Ala Ile Leu Asn Gly Ile Ala Asp Thr Trp Asn Arg Trp Ser 
                405                 410                 415     


Met Val Lys Lys Asp Tyr Asn Gly Asn Ile Ile Thr Asp Tyr Phe Asn 
            420                 425                 430         


Glu Asn Thr Asp Thr Tyr Lys Leu Ile Ser Lys Leu Asn Ser Phe Arg 
        435                 440                 445             


Gln Lys Tyr Glu Ala Leu Arg Glu Gly Thr Gln Arg Glu Met Trp Ser 
    450                 455                 460                 


Ser Pro His Leu Tyr Ala Phe Ser Arg Arg Met Asp Ser Gly Glu Asn 
465                 470                 475                 480 


Val Gly Gln Glu Val Val Asn Val Phe Asn Asn Ser Asp Gly Asp Gln 
                485                 490                 495     


Ser Ala Thr Ile Pro Ile Arg Ala Glu Ser Thr Ile Lys Val Gly Asp 
            500                 505                 510         


Lys Leu Val Asn Leu Phe Asp Val Asn Asp Ser Ile Thr Val Gln Gln 
        515                 520                 525             


Gly Gly Val Thr Gly Lys Gln Ile Ser Val Asn Leu Gly Glu Asn Ser 
    530                 535                 540                 


Gly Lys Ile Tyr Val Val Asn Asn Glu Thr Pro Asn Pro Asp Gln Lys 
545                 550                 555                 560 


Asn Val Gln Tyr Lys Val Ser Tyr Lys Asn Thr Asn Ala Gln Lys Val 
                565                 570                 575     


Thr Leu His Tyr Gly Thr Asn Gly Trp Lys Asn Ile Gln Asp Val Asn 
            580                 585                 590         


Met Thr Lys Asn Ser Asn Gly Glu Phe Glu Ala Thr Ile Thr Val Asn 
        595                 600                 605             


Asn Asn Asp Ile Leu Asn Tyr Cys Ile His Ile Ile Ser Pro Thr Asp 
    610                 615                 620                 


Tyr Trp Asp Asn Asn Gly Gly Gln Asn Trp Asn Val Lys Val Thr Lys 
625                 630                 635                 640 


Ala Glu Asp Tyr Ile Asn Asp Gly Val Lys Ser Asn Leu Lys Ser Val 
                645                 650                 655     


Asn Thr Thr Thr Ser Ala Ala Ile Glu Ser Gly Ile Asp Ser Thr Val 
            660                 665                 670         


Asn Arg 
        


<210>  4
<211>  2025
<212>  DNA
<213>  Clostridium saccharoperbutylacetonicum

<400>  4
atgtttagaa gaaaatttaa caaggtaata ttatctattt tagttgcaac aattgtttca       60

agcactaaca tgtttatgag tggaagcaag gcacaagcgg caattggaaa tttaagtgaa      120

aacgatacta tttatcaaat tatggtagac agattttatg atggagataa aacaaataat      180

gctacaggag atgcatttcg taatacagaa aatcttgaag atgattttag atatatgcac      240

ggcggagatt ggcaaggtgt tattgataag ttagattata ttaagggcat gggatactca      300

gccatttgga tatcaccggt tgcggaacca caaatgtggt ctagagctga tggcacagga      360

aaagtatggc ctacagctta ccatggatat aatgtgaaag atcccaataa ggcaaatcct      420

tattttggaa caaaagaaaa gctaaaggag ttagtagata aagctcacga aaaggggatt      480

aaagtaataa tagatatagt tccaaatcat gttggggatt atatgttagg aaaacaagct      540

tattatgaca tcaaggggtt tgagccggca gcacctttta ataatccaaa ttggtatcat      600

cataatggcg atattgattg gtcaagagaa cactctgatc cccaaatgtt agatgatcat      660

gatttgggcg gtttagatga tttaaatcaa gataattctg atgctaaggc agctatgaat      720

aatgctatta agtcatggtt tgattatact ggagctgatg cagcaagggt tgacgcagca      780

aaatgtatga aaccatctta tattaacgag ttacaaaagt atataggagt taatactttt      840

ggagaaaatt ttgatatgaa tgtagatttt gtgaagaagt gggttggatc cgatgcagaa      900

tggggaatgc tagattttcc attatatcaa gcaataaata atgattttgc atcaggacaa      960

tcttttgatg acatgtcatc atcaggtact tgctctatta aaaatatttt agcacaagac     1020

aataaatata atggttatgc aaatcatatg gtgactttta tagataatca tgatcgtaat     1080

agatttttaa cagtagcaaa tggtaatgtt aaaaaacttc aaaatgcact tgttttcatg     1140

tttactgtaa gaggggtacc aacagtattt caaggtacag aacaaaacaa aggtaatgga     1200

aatggagcaa ttctaaatgg tattgcagat acatggaatc gttggtcaat ggttaaaaag     1260

gactataatg gaaatataat tacagattat tttaatgaga atacagatac ttataaacta     1320

attagcaaat tgaattcatt taggcaaaaa tatgaagcct taagagaagg tactcaaaga     1380

gaaatgtggt cttcaccaca tttatatgca ttctcaagaa ggatggattc aggagaaaat     1440

gttggacaag aagttgtaaa tgtatttaat aattcagatg gagatcaaag tgcgaccatt     1500

ccaattagag ctgaaagtac tataaaagtt ggagataaac ttgtaaatct ttttgatgta     1560

aatgattcga tcacagttca acaaggaggt gttacaggaa aacaaatatc agtgaattta     1620

ggagaaaata gtgggaagat ttatgttgtt aataatgaaa caccaaatcc agatcaaaag     1680

aacgtacaat ataaagtttc atataagaat actaatgcac aaaaagtaac acttcattat     1740

ggaactaatg gatggaaaaa cattcaagat gtaaatatga ctaagaattc caatggagaa     1800

tttgaagcaa ctattacagt aaataataat gatattctaa attactgtat tcatattatt     1860

tcaccaacag actattggga taataatggt ggacagaatt ggaatgtaaa agtgactaag     1920

gcagaagatt atataaatga tggtgtaaag agtaatttga agagcgttaa tacaactaca     1980

tcagcagcga tagaatctgg tattgatagt actgtaaatc gttaa                     2025


