                         SEQUENCE LISTING

<110>  Cornell University
 
<120>  COMPOSITIONS AND METHODS FOR THE EXPANSION OF STEM CELLS

<130>  29727 (4849-02)

<150>  61/758,541
<151>  2013-01-30

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  251
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Met Ser Ser Phe Leu Ile Asn Ser Asn Tyr Val Asp Pro Lys 
1               5                   10                  15      


Phe Pro Pro Cys Glu Glu Tyr Ser Gln Ser Asp Tyr Leu Pro Ser Asp 
            20                  25                  30          


His Ser Pro Gly Tyr Tyr Ala Gly Gly Gln Arg Arg Glu Ser Ser Phe 
        35                  40                  45              


Gln Pro Glu Ala Gly Phe Gly Arg Arg Ala Ala Cys Thr Val Gln Arg 
    50                  55                  60                  


Tyr Ala Ala Cys Arg Asp Pro Gly Pro Pro Pro Pro Pro Pro Pro Pro 
65                  70                  75                  80  


Pro Pro Pro Pro Pro Pro Pro Gly Leu Ser Pro Arg Ala Pro Ala Pro 
                85                  90                  95      


Pro Pro Ala Gly Ala Leu Leu Pro Glu Pro Gly Gln Arg Cys Glu Ala 
            100                 105                 110         


Val Ser Ser Ser Pro Pro Pro Pro Pro Cys Ala Gln Asn Pro Leu His 
        115                 120                 125             


Pro Ser Pro Ser His Ser Ala Cys Lys Glu Pro Val Val Tyr Pro Trp 
    130                 135                 140                 


Met Arg Lys Val His Val Ser Thr Val Asn Pro Asn Tyr Ala Gly Gly 
145                 150                 155                 160 


Glu Pro Lys Arg Ser Arg Thr Ala Tyr Thr Arg Gln Gln Val Leu Glu 
                165                 170                 175     


Leu Glu Lys Glu Phe His Tyr Asn Arg Tyr Leu Thr Arg Arg Arg Arg 
            180                 185                 190         


Val Glu Ile Ala His Ala Leu Cys Leu Ser Glu Arg Gln Ile Lys Ile 
        195                 200                 205             


Trp Phe Gln Asn Arg Arg Met Lys Trp Lys Lys Asp His Lys Leu Pro 
    210                 215                 220                 


Asn Thr Lys Ile Arg Ser Gly Gly Ala Ala Gly Ser Ala Gly Gly Pro 
225                 230                 235                 240 


Pro Gly Arg Pro Asn Gly Gly Pro Arg Ala Leu 
                245                 250     


<210>  2
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human wild-type HOX LEXE motif within the first alpha helical 
       domain of the HOXB4 homeodomain  region


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  2

Leu Glu Xaa Glu 
1               


<210>  3
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant human HOXB4 protein sequence, including an amino acid 
       substitution at position 175, 176 and 178


<220>
<221>  MISC_FEATURE
<222>  (175)..(175)
<223>  Xaa: G, P, A, V, I, M, C, F, Y, W, H, K, R, Q, N, E, D, S or T

<220>
<221>  MISC_FEATURE
<222>  (176)..(176)
<223>  Xaa: G, P, A, V, I, M, C, F, Y, W, H, K, R, Q, N, D, L, S or T

<220>
<221>  MISC_FEATURE
<222>  (178)..(178)
<223>  Xaa: G, P, A, V, I, M, C, F, Y, W, H, K, R, Q, N, D, L, S or T

<400>  3

Met Ala Met Ser Ser Phe Leu Ile Asn Ser Asn Tyr Val Asp Pro Lys 
1               5                   10                  15      


Phe Pro Pro Cys Glu Glu Tyr Ser Gln Ser Asp Tyr Leu Pro Ser Asp 
            20                  25                  30          


His Ser Pro Gly Tyr Tyr Ala Gly Gly Gln Arg Arg Glu Ser Ser Phe 
        35                  40                  45              


Gln Pro Glu Ala Gly Phe Gly Arg Arg Ala Ala Cys Thr Val Gln Arg 
    50                  55                  60                  


Tyr Ala Ala Cys Arg Asp Pro Gly Pro Pro Pro Pro Pro Pro Pro Pro 
65                  70                  75                  80  


Pro Pro Pro Pro Pro Pro Pro Gly Leu Ser Pro Arg Ala Pro Ala Pro 
                85                  90                  95      


Pro Pro Ala Gly Ala Leu Leu Pro Glu Pro Gly Gln Arg Cys Glu Ala 
            100                 105                 110         


Val Ser Ser Ser Pro Pro Pro Pro Pro Cys Ala Gln Asn Pro Leu His 
        115                 120                 125             


Pro Ser Pro Ser His Ser Ala Cys Lys Glu Pro Val Val Tyr Pro Trp 
    130                 135                 140                 


Met Arg Lys Val His Val Ser Thr Val Asn Pro Asn Tyr Ala Gly Gly 
145                 150                 155                 160 


Glu Pro Lys Arg Ser Arg Thr Ala Tyr Thr Arg Gln Gln Val Xaa Xaa 
                165                 170                 175     


Leu Xaa Lys Glu Phe His Tyr Asn Arg Tyr Leu Thr Arg Arg Arg Arg 
            180                 185                 190         


Val Glu Ile Ala His Ala Leu Cys Leu Ser Glu Arg Gln Ile Lys Ile 
        195                 200                 205             


Trp Phe Gln Asn Arg Arg Met Lys Trp Lys Lys Asp His Lys Leu Pro 
    210                 215                 220                 


Asn Thr Lys Ile Arg Ser Gly Gly Ala Ala Gly Ser Ala Gly Gly Pro 
225                 230                 235                 240 


Pro Gly Arg Pro Asn Gly Gly Pro Arg Ala Leu 
                245                 250     


<210>  4
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant human HOXB4 protein sequence, including L175D, E176K and 
       E178K amino acid substitutions

<400>  4

Met Ala Met Ser Ser Phe Leu Ile Asn Ser Asn Tyr Val Asp Pro Lys 
1               5                   10                  15      


Phe Pro Pro Cys Glu Glu Tyr Ser Gln Ser Asp Tyr Leu Pro Ser Asp 
            20                  25                  30          


His Ser Pro Gly Tyr Tyr Ala Gly Gly Gln Arg Arg Glu Ser Ser Phe 
        35                  40                  45              


Gln Pro Glu Ala Gly Phe Gly Arg Arg Ala Ala Cys Thr Val Gln Arg 
    50                  55                  60                  


Tyr Ala Ala Cys Arg Asp Pro Gly Pro Pro Pro Pro Pro Pro Pro Pro 
65                  70                  75                  80  


Pro Pro Pro Pro Pro Pro Pro Gly Leu Ser Pro Arg Ala Pro Ala Pro 
                85                  90                  95      


Pro Pro Ala Gly Ala Leu Leu Pro Glu Pro Gly Gln Arg Cys Glu Ala 
            100                 105                 110         


Val Ser Ser Ser Pro Pro Pro Pro Pro Cys Ala Gln Asn Pro Leu His 
        115                 120                 125             


Pro Ser Pro Ser His Ser Ala Cys Lys Glu Pro Val Val Tyr Pro Trp 
    130                 135                 140                 


Met Arg Lys Val His Val Ser Thr Val Asn Pro Asn Tyr Ala Gly Gly 
145                 150                 155                 160 


Glu Pro Lys Arg Ser Arg Thr Ala Tyr Thr Arg Gln Gln Val Asp Lys 
                165                 170                 175     


Leu Lys Lys Glu Phe His Tyr Asn Arg Tyr Leu Thr Arg Arg Arg Arg 
            180                 185                 190         


Val Glu Ile Ala His Ala Leu Cys Leu Ser Glu Arg Gln Ile Lys Ile 
        195                 200                 205             


Trp Phe Gln Asn Arg Arg Met Lys Trp Lys Lys Asp His Lys Leu Pro 
    210                 215                 220                 


Asn Thr Lys Ile Arg Ser Gly Gly Ala Ala Gly Ser Ala Gly Gly Pro 
225                 230                 235                 240 


Pro Gly Arg Pro Asn Gly Gly Pro Arg Ala Leu 
                245                 250     


<210>  5
<211>  272
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Ala Thr Thr Gly Ala Leu Gly Asn Tyr Tyr Val Asp Ser Phe Leu 
1               5                   10                  15      


Leu Gly Ala Asp Ala Ala Asp Glu Leu Ser Val Gly Arg Tyr Ala Pro 
            20                  25                  30          


Gly Thr Leu Gly Gln Pro Pro Arg Gln Ala Ala Thr Leu Ala Glu His 
        35                  40                  45              


Pro Asp Phe Ser Pro Cys Ser Phe Gln Ser Lys Ala Thr Val Phe Gly 
    50                  55                  60                  


Ala Ser Trp Asn Pro Val His Ala Ala Gly Ala Asn Ala Val Pro Ala 
65                  70                  75                  80  


Ala Val Tyr His His His His His His Pro Tyr Val His Pro Gln Ala 
                85                  90                  95      


Pro Val Ala Ala Ala Ala Pro Asp Gly Arg Tyr Met Arg Ser Trp Leu 
            100                 105                 110         


Glu Pro Thr Pro Gly Ala Leu Ser Phe Ala Gly Leu Pro Ser Ser Arg 
        115                 120                 125             


Pro Tyr Gly Ile Lys Pro Glu Pro Leu Ser Ala Arg Arg Gly Asp Cys 
    130                 135                 140                 


Pro Thr Leu Asp Thr His Thr Leu Ser Leu Thr Asp Tyr Ala Cys Gly 
145                 150                 155                 160 


Ser Pro Pro Val Asp Arg Glu Lys Gln Pro Ser Glu Gly Ala Phe Ser 
                165                 170                 175     


Glu Asn Asn Ala Glu Asn Glu Ser Gly Gly Asp Lys Pro Pro Ile Asp 
            180                 185                 190         


Pro Asn Asn Pro Ala Ala Asn Trp Leu His Ala Arg Ser Thr Arg Lys 
        195                 200                 205             


Lys Arg Cys Pro Tyr Thr Lys His Gln Thr Leu Glu Leu Glu Lys Glu 
    210                 215                 220                 


Phe Leu Phe Asn Met Tyr Leu Thr Arg Asp Arg Arg Tyr Glu Val Ala 
225                 230                 235                 240 


Arg Leu Leu Asn Leu Thr Glu Arg Gln Val Lys Ile Trp Phe Gln Asn 
                245                 250                 255     


Arg Arg Met Lys Met Lys Lys Ile Asn Lys Asp Arg Ala Lys Asp Glu 
            260                 265                 270         


<210>  6
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant HOXA9 protein sequence, including an amino acid 
       substitution at position 219, 220, or 222


<220>
<221>  MISC_FEATURE
<222>  (219)..(219)
<223>  Xaa: G, P, A, V, I, M, C, F, Y, W, H, K, R, Q, N, E, D, S or T

<220>
<221>  MISC_FEATURE
<222>  (220)..(220)
<223>  Xaa: G, P, A, V, I, M, C, F, Y, W, H, K, R, Q, N, E, D, S or T

<220>
<221>  MISC_FEATURE
<222>  (222)..(222)
<223>  Xaa: G, P, A, V, I, M, C, F, Y, W, H, K, R, Q, N, D, L, S or T

<400>  6

Met Ala Thr Thr Gly Ala Leu Gly Asn Tyr Tyr Val Asp Ser Phe Leu 
1               5                   10                  15      


Leu Gly Ala Asp Ala Ala Asp Glu Leu Ser Val Gly Arg Tyr Ala Pro 
            20                  25                  30          


Gly Thr Leu Gly Gln Pro Pro Arg Gln Ala Ala Thr Leu Ala Glu His 
        35                  40                  45              


Pro Asp Phe Ser Pro Cys Ser Phe Gln Ser Lys Ala Thr Val Phe Gly 
    50                  55                  60                  


Ala Ser Trp Asn Pro Val His Ala Ala Gly Ala Asn Ala Val Pro Ala 
65                  70                  75                  80  


Ala Val Tyr His His His His His His Pro Tyr Val His Pro Gln Ala 
                85                  90                  95      


Pro Val Ala Ala Ala Ala Pro Asp Gly Arg Tyr Met Arg Ser Trp Leu 
            100                 105                 110         


Glu Pro Thr Pro Gly Ala Leu Ser Phe Ala Gly Leu Pro Ser Ser Arg 
        115                 120                 125             


Pro Tyr Gly Ile Lys Pro Glu Pro Leu Ser Ala Arg Arg Gly Asp Cys 
    130                 135                 140                 


Pro Thr Leu Asp Thr His Thr Leu Ser Leu Thr Asp Tyr Ala Cys Gly 
145                 150                 155                 160 


Ser Pro Pro Val Asp Arg Glu Lys Gln Pro Ser Glu Gly Ala Phe Ser 
                165                 170                 175     


Glu Asn Asn Ala Glu Asn Glu Ser Gly Gly Asp Lys Pro Pro Ile Asp 
            180                 185                 190         


Pro Asn Asn Pro Ala Ala Asn Trp Leu His Ala Arg Ser Thr Arg Lys 
        195                 200                 205             


Lys Arg Cys Pro Tyr Thr Lys His Gln Thr Xaa Xaa Leu Xaa Lys Glu 
    210                 215                 220                 


Phe Leu Phe Asn Met Tyr Leu Thr Arg Asp Arg Arg Tyr Glu Val Ala 
225                 230                 235                 240 


Arg Leu Leu Asn Leu Thr Glu Arg Gln Val Lys Ile Trp Phe Gln Asn 
                245                 250                 255     


Arg Arg Met Lys Met Lys Lys Ile Asn Lys Asp Arg Ala Lys Asp Glu 
            260                 265                 270         


<210>  7
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant human HOXA9 protein sequence, including amino acid 
       substitutions L219D, E220K and E222K

<400>  7

Met Ala Thr Thr Gly Ala Leu Gly Asn Tyr Tyr Val Asp Ser Phe Leu 
1               5                   10                  15      


Leu Gly Ala Asp Ala Ala Asp Glu Leu Ser Val Gly Arg Tyr Ala Pro 
            20                  25                  30          


Gly Thr Leu Gly Gln Pro Pro Arg Gln Ala Ala Thr Leu Ala Glu His 
        35                  40                  45              


Pro Asp Phe Ser Pro Cys Ser Phe Gln Ser Lys Ala Thr Val Phe Gly 
    50                  55                  60                  


Ala Ser Trp Asn Pro Val His Ala Ala Gly Ala Asn Ala Val Pro Ala 
65                  70                  75                  80  


Ala Val Tyr His His His His His His Pro Tyr Val His Pro Gln Ala 
                85                  90                  95      


Pro Val Ala Ala Ala Ala Pro Asp Gly Arg Tyr Met Arg Ser Trp Leu 
            100                 105                 110         


Glu Pro Thr Pro Gly Ala Leu Ser Phe Ala Gly Leu Pro Ser Ser Arg 
        115                 120                 125             


Pro Tyr Gly Ile Lys Pro Glu Pro Leu Ser Ala Arg Arg Gly Asp Cys 
    130                 135                 140                 


Pro Thr Leu Asp Thr His Thr Leu Ser Leu Thr Asp Tyr Ala Cys Gly 
145                 150                 155                 160 


Ser Pro Pro Val Asp Arg Glu Lys Gln Pro Ser Glu Gly Ala Phe Ser 
                165                 170                 175     


Glu Asn Asn Ala Glu Asn Glu Ser Gly Gly Asp Lys Pro Pro Ile Asp 
            180                 185                 190         


Pro Asn Asn Pro Ala Ala Asn Trp Leu His Ala Arg Ser Thr Arg Lys 
        195                 200                 205             


Lys Arg Cys Pro Tyr Thr Lys His Gln Thr Asp Lys Leu Lys Lys Glu 
    210                 215                 220                 


Phe Leu Phe Asn Met Tyr Leu Thr Arg Asp Arg Arg Tyr Glu Val Ala 
225                 230                 235                 240 


Arg Leu Leu Asn Leu Thr Glu Arg Gln Val Lys Ile Trp Phe Gln Asn 
                245                 250                 255     


Arg Arg Met Lys Met Lys Lys Ile Asn Lys Asp Arg Ala Lys Asp Glu 
            260                 265                 270         


