
                                SEQUENCE LISTING

<110> Glaxo Group Limited

<120> METHOD OF PRODUCING A PROTEIN
  

<130> PB65373

<160> 10

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 163
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of dAt01 (Exendin4-Dom7h-14-10
      AlbudAb)

<400> 1
His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu
 1               5                  10                  15      
Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser
            20                  25                  30          
Ser Gly Ala Pro Pro Pro Ser Gly Gly Gly Gly Gly Ser Gly Gly Gly
        35                  40                  45              
Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser
    50                  55                  60                  
Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala
65                  70                  75                  80  
Ser Gln Trp Ile Gly Ser Gln Leu Ser Trp Tyr Gln Gln Lys Pro Gly
                85                  90                  95      
Lys Ala Pro Lys Leu Leu Ile Met Trp Arg Ser Ser Leu Gln Ser Gly
            100                 105                 110         
Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu
        115                 120                 125             
Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Ala
    130                 135                 140                 
Gln Gly Leu Arg His Pro Lys Thr Phe Gly Gln Gly Thr Lys Val Glu
145                 150                 155                 160 
Ile Lys Arg
            


<210> 2
<211> 489
<212> DNA
<213> Artificial Sequence

<220> 
<223> DNA sequence of dAt01 (Exendin4-Dom7h-14-10
      AlbudAb)

<400> 2
cacggtgaag gtacgttcac ctctgacctg agcaaacaga tggaggaaga agcggttcgt 60
ctgttcatcg agtggctgaa aaacggtggt ccgtcttctg gtgctccgcc gccgtctggt 120
ggtggtggtg gttctggtgg tggtggttct ggtggtggtg gcagcgatat ccagatgact 180
cagtccccgt cttctctctc cgcctctgtt ggcgaccgtg ttaccatcac ttgtcgtgcg 240
agccagtgga tcggttccca gctgagctgg tatcagcaga aaccgggcaa agcgccgaaa 300
ctgctgatca tgtggcgctc tagcctgcag tctggtgtac cgtctcgttt ctccggctct 360
ggttctggta cggacttcac cctcacgatc tcttccctgc agccggaaga ctttgccacc 420
tactactgcg cacagggtct gcgtcacccg aaaaccttcg gtcagggtac caaagtcgag 480
atcaaacgt                                                         489

<210> 3
<211> 108
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of dAt06 (Dom7h-11-15 R108C)
      AlbudAb

<400> 3
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
 1               5                  10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Arg Pro Ile Gly Thr Met
            20                  25                  30          
Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
        35                  40                  45              
Leu Ala Phe Ser Arg Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Gln Ala Gly Thr His Pro Thr
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Cys
            100                 105             


<210> 4
<211> 324
<212> DNA
<213> Artificial Sequence

<220> 
<223> DNA sequence of dAt06 (Dom7h-11-15 R108C) AlbudAb

<400> 4
gatatccaga tgacccagtc tccgtcttcc ctgtctgcgt ctgttggtga tcgcgttacc 60
atcacttgcc gtgcaagccg tccgatcggt actatgctga gctggtacca gcagaaaccg 120
ggtaaagcgc cgaaactgct gattctggct ttctctcgcc tgcagtctgg tgttccgtct 180
cgtttcagcg gtagcggttc tggtaccgac ttcaccctga ccatttcctc tctgcagccg 240
gaagacttcg ctacctacta ttgtgcgcag gcaggtactc acccgactac cttcggtcag 300
ggcaccaaag ttgaaatcaa atgc                                        324

<210> 5
<211> 230
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of DOM100 (DMS5541) AlbudAb
      -TNFR1

<400> 5
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Lys Tyr
            20                  25                  30          
Ser Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
        35                  40                  45              
Ser Gln Ile Ser Asp Thr Ala Asp Arg Thr Tyr Tyr Ala His Ala Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ala Ile Tyr Thr Gly Arg Trp Val Pro Phe Glu Tyr Trp Gly Gln Gly
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser Ala Ser Thr Asp Ile Gln Met Thr Gln
        115                 120                 125             
Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr
    130                 135                 140                 
Cys Arg Ala Ser Arg Pro Ile Gly Thr Thr Leu Ser Trp Tyr Gln Gln
145                 150                 155                 160 
Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Leu Trp Asn Ser Arg Leu
                165                 170                 175     
Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp
            180                 185                 190         
Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr
        195                 200                 205             
Tyr Cys Ala Gln Ala Gly Thr His Pro Thr Thr Phe Gly Gln Gly Thr
    210                 215                 220                 
Lys Val Glu Ile Lys Arg
225                 230 


<210> 6
<211> 690
<212> DNA
<213> Artificial Sequence

<220> 
<223> DNA sequence of DOM100 (DMS5541) AlbudAb -TNFR1

<400> 6
gaggtacagc tgctggaatc tggtggtggt ctggttcagc cgggtggctc tctgcgtctg 60
tcttgtgcag cgtctggttt caccttcgac aaatactcta tgggctgggt tcgtcaggcg 120
ccgggtaaag gtctggaatg ggtgtctcag atctctgaca ccgcagatcg tacctactac 180
gcacacgctg tgaaaggtcg cttcaccatc tctcgcgaca actccaaaaa caccctgtac 240
ctgcagatga actccctgcg tgctgaagac accgcggtat actattgcgc gatctacacc 300
ggtcgttggg ttccgttcga atactggggt cagggtaccc tggttactgt gagctctgcg 360
tctaccgaca tccagatgac ccagtctccg tcttctctgt ctgcgagcgt tggtgaccgt 420
gttaccatca cttgccgtgc ttctcgtccg atcggtacca ctctgagctg gtatcagcag 480
aaaccgggca aagcgccgaa actgctgatc ctgtggaact ctcgtctgca gtccggtgtt 540
ccgtctcgtt tctctggcag cggttctggt accgacttca ccctgactat ctctagcctg 600
cagccggaag acttcgcaac ctactattgc gcacaggctg gtactcaccc gaccactttc 660
ggtcagggta ccaaagtaga aatcaaacgt                                  690

<210> 7
<211> 119
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of DOM101

<400> 7
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ala His Glu
            20                  25                  30          
Thr Met Val Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
        35                  40                  45              
Ser His Ile Pro Pro Asp Gly Gln Asp Pro Phe Tyr Ala Asp Ser Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr His Cys
                85                  90                  95      
Ala Leu Leu Pro Lys Arg Gly Pro Trp Phe Asp Tyr Trp Gly Gln Gly
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser
        115                 


<210> 8
<211> 357
<212> DNA
<213> Artificial Sequence

<220> 
<223> DNA sequence of DOM101

<400> 8
gaagtacaac tgctggagag cggtggcggc ctggttcaac cgggtggttc cctgcgcctg 60
tcctgtgcgg catctggttt caccttcgca cacgaaacca tggtgtgggt tcgccaagct 120
ccgggcaaag gcctggaatg ggtaagccac attcctccag atggccagga cccattctat 180
gcggattccg ttaagggtcg ctttaccatt tctcgtgata actccaaaaa caccctgtac 240
ctgcagatga actccctgcg cgccgaggat actgcggtgt accattgtgc gctgctgcct 300
aaacgtggcc cgtggttcga ttactggggt cagggtactc tggtcaccgt aagcagc    357

<210> 9
<211> 120
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of alanine extended DOM101
      

<400> 9
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ala His Glu
            20                  25                  30          
Thr Met Val Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
        35                  40                  45              
Ser His Ile Pro Pro Asp Gly Gln Asp Pro Phe Tyr Ala Asp Ser Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr His Cys
                85                  90                  95      
Ala Leu Leu Pro Lys Arg Gly Pro Trp Phe Asp Tyr Trp Gly Gln Gly
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser Ala
        115                 120 


<210> 10
<211> 360
<212> DNA
<213> Artificial Sequence

<220> 
<223> DNA sequence of alanine extended DOM101
      

<400> 10
gaagtacaac tgctggagag cggtggcggc ctggttcaac cgggtggttc cctgcgcctg 60
tcctgtgcgg catctggttt caccttcgca cacgaaacca tggtgtgggt tcgccaagct 120
ccgggcaaag gcctggaatg ggtaagccac attcctccag atggccagga cccattctat 180
gcggattccg ttaagggtcg ctttaccatt tctcgtgata actccaaaaa caccctgtac 240
ctgcagatga actccctgcg cgccgaggat actgcggtgt accattgtgc gctgctgcct 300
aaacgtggcc cgtggttcga ttactggggt cagggtactc tggtcaccgt aagcagcgcg 360

