                         SEQUENCE LISTING

<110>  Thrombotargets Europe SL
 
<120>  Hemostatic Compositions

<130>  P2553EP00

<150>  EP  13152475.3
<151>  2013-01-24

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  263
<212>  PRT
<213>  Homo sapiens

<400>  1

Ser Gly Thr Thr Asn Thr Val Ala Ala Tyr Asn Leu Thr Trp Lys Ser 
1               5                   10                  15      


Thr Asn Phe Lys Thr Ile Leu Glu Trp Glu Pro Lys Pro Val Asn Gln 
            20                  25                  30          


Val Tyr Thr Val Gln Ile Ser Thr Lys Ser Gly Asp Trp Lys Ser Lys 
        35                  40                  45              


Cys Phe Tyr Thr Thr Asp Thr Glu Cys Asp Leu Thr Asp Glu Ile Val 
    50                  55                  60                  


Lys Asp Val Lys Gln Thr Tyr Leu Ala Arg Val Phe Ser Tyr Pro Ala 
65                  70                  75                  80  


Gly Asn Val Glu Ser Thr Gly Ser Ala Gly Glu Pro Leu Tyr Glu Asn 
                85                  90                  95      


Ser Pro Glu Phe Thr Pro Tyr Leu Glu Thr Asn Leu Gly Gln Pro Thr 
            100                 105                 110         


Ile Gln Ser Phe Glu Gln Val Gly Thr Lys Val Asn Val Thr Val Glu 
        115                 120                 125             


Asp Glu Arg Thr Leu Val Arg Arg Asn Asn Thr Phe Leu Ser Leu Arg 
    130                 135                 140                 


Asp Val Phe Gly Lys Asp Leu Ile Tyr Thr Leu Tyr Tyr Trp Lys Ser 
145                 150                 155                 160 


Ser Ser Ser Gly Lys Lys Thr Ala Lys Thr Asn Thr Asn Glu Phe Leu 
                165                 170                 175     


Ile Asp Val Asp Lys Gly Glu Asn Tyr Cys Phe Ser Val Gln Ala Val 
            180                 185                 190         


Ile Pro Ser Arg Thr Val Asn Arg Lys Ser Thr Asp Ser Pro Val Glu 
        195                 200                 205             


Cys Met Gly Gln Glu Lys Gly Glu Phe Arg Glu Ile Phe Tyr Ile Ile 
    210                 215                 220                 


Gly Ala Val Val Phe Val Val Ile Ile Leu Val Ile Ile Leu Ala Ile 
225                 230                 235                 240 


Ser Leu His Lys Cys Arg Lys Ala Gly Val Gly Gln Ser Trp Lys Glu 
                245                 250                 255     


Asn Ser Pro Leu Asn Val Ser 
            260             


<210>  2
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  epitope AHGHRP

<400>  2

Ala His Gly His Arg Pro 
1               5       


<210>  3
<211>  14
<212>  PRT
<213>  artificial sequence

<220>
<223>  epitope

<400>  3

Pro Ile His Asp His Asp His Pro His Leu Val Ile His Ser 
1               5                   10                  


<210>  4
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  epitope wherein Xaa can be any naturally occurring amino acid


<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  Xaa can be any naturally occurring amino acid

<400>  4

Gly Met Thr Cys Xaa Xaa Cys 
1               5           


<210>  5
<211>  269
<212>  PRT
<213>  artificial sequence

<220>
<223>  Human mature TF with N124A mutation and C-terminal hexahistidine

<400>  5

Ser Gly Thr Thr Asn Thr Val Ala Ala Tyr Asn Leu Thr Trp Lys Ser 
1               5                   10                  15      


Thr Asn Phe Lys Thr Ile Leu Glu Trp Glu Pro Lys Pro Val Asn Gln 
            20                  25                  30          


Val Tyr Thr Val Gln Ile Ser Thr Lys Ser Gly Asp Trp Lys Ser Lys 
        35                  40                  45              


Cys Phe Tyr Thr Thr Asp Thr Glu Cys Asp Leu Thr Asp Glu Ile Val 
    50                  55                  60                  


Lys Asp Val Lys Gln Thr Tyr Leu Ala Arg Val Phe Ser Tyr Pro Ala 
65                  70                  75                  80  


Gly Asn Val Glu Ser Thr Gly Ser Ala Gly Glu Pro Leu Tyr Glu Asn 
                85                  90                  95      


Ser Pro Glu Phe Thr Pro Tyr Leu Glu Thr Asn Leu Gly Gln Pro Thr 
            100                 105                 110         


Ile Gln Ser Phe Glu Gln Val Gly Thr Lys Val Ala Val Thr Val Glu 
        115                 120                 125             


Asp Glu Arg Thr Leu Val Arg Arg Asn Asn Thr Phe Leu Ser Leu Arg 
    130                 135                 140                 


Asp Val Phe Gly Lys Asp Leu Ile Tyr Thr Leu Tyr Tyr Trp Lys Ser 
145                 150                 155                 160 


Ser Ser Ser Gly Lys Lys Thr Ala Lys Thr Asn Thr Asn Glu Phe Leu 
                165                 170                 175     


Ile Asp Val Asp Lys Gly Glu Asn Tyr Cys Phe Ser Val Gln Ala Val 
            180                 185                 190         


Ile Pro Ser Arg Thr Val Asn Arg Lys Ser Thr Asp Ser Pro Val Glu 
        195                 200                 205             


Cys Met Gly Gln Glu Lys Gly Glu Phe Arg Glu Ile Phe Tyr Ile Ile 
    210                 215                 220                 


Gly Ala Val Val Phe Val Val Ile Ile Leu Val Ile Ile Leu Ala Ile 
225                 230                 235                 240 


Ser Leu His Lys Cys Arg Lys Ala Gly Val Gly Gln Ser Trp Lys Glu 
                245                 250                 255     


Asn Ser Pro Leu Asn Val Ser His His His His His His 
            260                 265                 


<210>  6
<211>  813
<212>  DNA
<213>  artificial sequence

<220>
<223>  DNA encoding human mature TF with N124A and C-terminal

<400>  6
atgtcaggca ctacaaatac tgtggcagca tataatttaa cttggaaatc aactaatttc       60

aagacaattt tggagtggga acccaaaccc gtcaatcaag tctacactgt tcaaataagc      120

actaagtcag gagattggaa aagcaaatgc ttttacacaa cagacacaga gtgtgacctc      180

accgacgaga ttgtgaagga tgtgaagcag acgtacttgg cacgggtctt ctcctacccg      240

gcagggaatg tggagagcac cggttctgct ggggagcctc tgtatgagaa ctccccagag      300

ttcacacctt acctggagac aaacctcgga cagccaacaa ttcagagttt tgaacaggtg      360

ggaacaaaag tggcagtgac cgtagaagat gaacggactt tagtcagaag gaacaacact      420

ttcctaagcc tccgggatgt ttttggcaag gacttaattt atacacttta ttattggaaa      480

tcttcaagtt caggaaagaa aacagccaaa acaaacacta atgagttttt gattgatgtg      540

gataaaggag aaaactactg tttcagtgtt caagcagtga ttccctcccg aacagttaac      600

cggaagagta cagacagccc ggtagagtgt atgggccagg agaaagggga attcagagaa      660

atattctaca tcattggagc tgtggtattt gtggtcatca tccttgtcat catcctggct      720

atatctctac acaagtgtag aaaggcagga gtggggcaga gctggaagga gaactcccca      780

ctgaatgttt cacatcacca tcaccatcac tag                                   813


<210>  7
<211>  33
<212>  DNA
<213>  artificial sequence

<220>
<223>  upstream primer encoding the first amino acids of mature TF 
       lacking the signal peptide and containing an initiation codon ATG
       in frame with TF ORF, and a restriction BamHI site

<400>  7
gggatccgca tgtcaggcac tacaaatact gtg                                    33


<210>  8
<211>  50
<212>  DNA
<213>  artificial sequence

<220>
<223>  downstream primer containing the histidine coding nucleotides  
       and a new termination signal TAG, both containing a restriction 
       BamHI site

<400>  8
cggatccgtg tcgacctagt gatggtgatg gtgatgtgaa acattcagtg                  50


<210>  9
<211>  33
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  9
gtgggaacaa aagtggcagt gaccgtagaa gat                                    33


<210>  10
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  10
atcttctacg gtcactgcca cttttgt                                           27


<210>  11
<211>  1374
<212>  DNA
<213>  Homo sapiens

<400>  11
gggggcgccg ccggcccttt atagcgcgcg gggcaccggc tccccaagac tgcgagctcc       60

ccgcaccccc tcgcactccc tctggccggc ccagggcgcc ttcagcccaa cctccccagc      120

cccacgggcg ccacggaacc cgctcgatct cgccgccaac tggtagacat ggagacccct      180

gcctggcccc gggtcccgcg ccccgagacc gccgtcgctc ggacgctcct gctcggctgg      240

gtcttcgccc aggtggccgg cgcttcaggc actacaaata ctgtggcagc atataattta      300

acttggaaat caactaattt caagacaatt ttggagtggg aacccaaacc cgtcaatcaa      360

gtctacactg ttcaaataag cactaagtca ggagattgga aaagcaaatg cttttacaca      420

acagacacag agtgtgacct caccgacgag attgtgaagg atgtgaagca gacgtacttg      480

gcacgggtct tctcctaccc ggcagggaat gtggagagca ccggttctgc tggggagcct      540

ctgtatgaga actccccaga gttcacacct tacctggaga caaacctcgg acagccaaca      600

attcagagtt ttgaacaggt gggaacaaaa gtgaatgtga ccgtagaaga tgaacggact      660

ttagtcagaa ggaacaacac tttcctaagc ctccgggatg tttttggcaa ggacttaatt      720

tatacacttt attattggaa atcttcaagt tcaggaaaga aaacagccaa aacaaacact      780

aatgagtttt tgattgatgt ggataaagga gaaaactact gtttcagtgt tcaagcagtg      840

attccctccc gaacagttaa ccggaagagt acagacagcc cggtagagtg tatgggccag      900

gagaaagggg aattcagaga aatattctac atcattggag ctgtggtatt tgtggtcatc      960

atccttgtca tcatcctggc tatatctcta cacaagtgta gaaaggcagg agtggggcag     1020

agctggaagg agaactcccc actgaatgtt tcataaagga agcactgttg gagctactgc     1080

aaatgctata ttgcactgtg accgagaact tttaagagga tagaatacat ggaaacgcaa     1140

atgagtattt cggagcatga agaccctgga gttcaaaaaa ctcttgatat gacctgttat     1200

taccattagc attctggttt tgacatcagc attagtcact ttgaaatgta acaaatggta     1260

ctacaaccaa ttccaagttt taatttttaa caccatggca ccttttgcac ataacatgct     1320

ttagattata tattccgccg tcccaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa           1374


