                         SEQUENCE LISTING

<110>  Consejo Superior de Investigaciones Cientficas
 
<120>  Stress Tolerant Plants

<130>  PC926790wo

<160>  40    

<170>  PatentIn version 3.3

<210>  1
<211>  1434
<212>  DNA
<213>  Helianthus annuus

<220>
<223>  HaHSFA9 full-length cDNA (Accession number AY099451)

<400>  1
atattctcct tcaaaaacac acacacacaa acaaacaaac ttgtttttga tttgtacttc     60

atggcaggag tagtaaaaga gtttgactct agtcatgata tgttcaaagt cagagaagaa    120

cccaaggtcg tttttaataa taaagatttg tctgaagaga tgatgaagat taaagaggaa    180

cccatgttag tgtttgatga gcatgaagat tttgttggtg gtgaggaggc accgaagccg    240

attgaagggc tgagagacgg cggacctccg ccgtttctca agaaaacatt tgaaatggtg    300

gatgacccaa caacagattc gattatttca tggagtagtt ctaaaaatag ttttattctt    360

tgggatcctc ataagttttc tactgatctt ttgccgcaaa gattcaagca caataatttc    420

tctagctttg ttcgtcaact aaacacttat aggttcaaga aaatcgatcc tgaccgatgg    480

gaattcgcaa acgagttttt ccaaaagggt aagaagcatt tgttaagaga cataaagagg    540

agaacaaacc agccacaaaa cacacaaaaa caagaggaaa tcaggaaaca agaacaacaa    600

caatgttgtg gtcatcaaac aaactctaca atggagaccg agctaaagaa tctaagaaaa    660

gaaagaatca cacttaaaca agagatcttg aagatgaaac agcaacaaga gaacaccgaa    720

aagcatttgg aaatggttga agaacgtatg ctaagaatgg agttcaagca acagcagctg    780

ttagttttca tgtctaaagc gtttagaaat ccgattttcg ttaagctttt gcaacatctg    840

gtgcaaaaac aaaaaacggg aagtgtcgaa atgtgcaaaa agcgaaaact tgagcaaatg    900

ctaaacactg atgatctcga tcgttttcaa gaaatgtgga atatgattga acctgatgcg    960

tatactgtgt tgtcatcaga tggatcagta agcccgcctg aagatcaaaa cacgagtgac   1020

aaatctggat ccaacggttc agattacaat tcggaaagct ttatattgtg ggagaaacta   1080

atggaggacg aactgatatt cggcggtgaa caatcgggta aagatcaaac agaaacttat   1140

ctccaagaat gggaagagtt gatcccaaaa gcttaagaag ggggattctg atgcagtcat   1200

gtgatcatta tcatcggtaa catgataaag agcatctggt agggtgctgc agtgctcggg   1260

taagctttat cttctggtac tgggcagttg atgtagatgt agaaacaaat ggggtacagt   1320

ataggtgttt gtgtgtgtgt tgtatttgtt tttgttgtat tttggttggg gtcttgtaga   1380

aataacgatg acaataaact ccgtagtttc taatagaatt tagggttgta aatc         1434


<210>  2
<211>  371
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  Predicted HaHSFA9 protein

<400>  2

Met Ala Gly Val Val Lys Glu Phe Asp Ser Ser His Asp Met Phe Lys 
1               5                   10                  15      


Val Arg Glu Glu Pro Lys Val Val Phe Asn Asn Lys Asp Leu Ser Glu 
            20                  25                  30          


Glu Met Met Lys Ile Lys Glu Glu Pro Met Leu Val Phe Asp Glu His 
        35                  40                  45              


Glu Asp Phe Val Gly Gly Glu Glu Ala Pro Lys Pro Ile Glu Gly Leu 
    50                  55                  60                  


Arg Asp Gly Gly Pro Pro Pro Phe Leu Lys Lys Thr Phe Glu Met Val 
65                  70                  75                  80  


Asp Asp Pro Thr Thr Asp Ser Ile Ile Ser Trp Ser Ser Ser Lys Asn 
                85                  90                  95      


Ser Phe Ile Leu Trp Asp Pro His Lys Phe Ser Thr Asp Leu Leu Pro 
            100                 105                 110         


Gln Arg Phe Lys His Asn Asn Phe Ser Ser Phe Val Arg Gln Leu Asn 
        115                 120                 125             


Thr Tyr Arg Phe Lys Lys Ile Asp Pro Asp Arg Trp Glu Phe Ala Asn 
    130                 135                 140                 


Glu Phe Phe Gln Lys Gly Lys Lys His Leu Leu Arg Asp Ile Lys Arg 
145                 150                 155                 160 


Arg Thr Asn Gln Pro Gln Asn Thr Gln Lys Gln Glu Glu Ile Arg Lys 
                165                 170                 175     


Gln Glu Gln Gln Gln Cys Cys Gly His Gln Thr Asn Ser Thr Met Glu 
            180                 185                 190         


Thr Glu Leu Lys Asn Leu Arg Lys Glu Arg Ile Thr Leu Lys Gln Glu 
        195                 200                 205             


Ile Leu Lys Met Lys Gln Gln Gln Glu Asn Thr Glu Lys His Leu Glu 
    210                 215                 220                 


Met Val Glu Glu Arg Met Leu Arg Met Glu Phe Lys Gln Gln Gln Leu 
225                 230                 235                 240 


Leu Val Phe Met Ser Lys Ala Phe Arg Asn Pro Ile Phe Val Lys Leu 
                245                 250                 255     


Leu Gln His Leu Val Gln Lys Gln Lys Thr Gly Ser Val Glu Met Cys 
            260                 265                 270         


Lys Lys Arg Lys Leu Glu Gln Met Leu Asn Thr Asp Asp Leu Asp Arg 
        275                 280                 285             


Phe Gln Glu Met Trp Asn Met Ile Glu Pro Asp Ala Tyr Thr Val Leu 
    290                 295                 300                 


Ser Ser Asp Gly Ser Val Ser Pro Pro Glu Asp Gln Asn Thr Ser Asp 
305                 310                 315                 320 


Lys Ser Gly Ser Asn Gly Ser Asp Tyr Asn Ser Glu Ser Phe Ile Leu 
                325                 330                 335     


Trp Glu Lys Leu Met Glu Asp Glu Leu Ile Phe Gly Gly Glu Gln Ser 
            340                 345                 350         


Gly Lys Asp Gln Thr Glu Thr Tyr Leu Gln Glu Trp Glu Glu Leu Ile 
        355                 360                 365             


Pro Lys Ala 
    370     


<210>  3
<211>  1516
<212>  DNA
<213>  Helianthus annuus

<220>
<223>  HaHSFA4 full-length cDNA (Accession number HF549285)

<400>  3
ggacactgac atggactgaa ggagtagaaa aagtagtgag tttcgacact tggggtgacg     60

tttcaagcac tttggaagcc attttggata atattttggt tcaggggatt aggattagac    120

tcatcaaaat tactttttag ctctgttttg gagttatttt tgggagtatt atcttgatgt    180

agtttggaaa ttgaagtgat cttgattgtt gttggtatat cttggtcaat gatgaatgat    240

gttcatggga atttgaattc gaattcgaat tcgggttcaa atgctctgcc cccttttatc    300

gcgaaaacgt atgaaatggt cgatgatcct tctacaaatt ccatcgtttc gtggagtcag    360

actaaccgga gtttcatcgt gtggaacccg cctgaattct cgggtgaatt gcttccaaga    420

ttcttcaagc ataacaattt ctcaagcttc atcagacaac ttaatactta tggttttcga    480

aaaatcgatc ctgaattatg ggaatttgct aatgaggatt tcattagagg tcaaccacat    540

ctgttaaaga acatccatag aagaaaaccg gttcatagtc attcgattca caatcttgca    600

aacaatggat catcatcgtc gtcgtctcca ttgaccgaat cagagaaaca acaatacata    660

gaaaagatta gcaccctgca acacgagaaa gaatcgttgt ctatacagtt tcacatgcac    720

aaacaagaac aggagaggat caaattcgaa gcacacgggt taacagaccg tttaaaacgg    780

gctgcaaagc tgcaaaaaga catcctttgt tgcttagacg agttcttgca gaaaccacct    840

caagaatgcg aattcacgcc tcggttgatg gagaatcgaa agaggagatt atcgtctgaa    900

aacgttaacg aacaagtttg tcctttcgat atcccgattc gcgagacgat aaccgccgac    960

gcgcttctag cgttgaacac cgaattggta gaacagttgg agtcctcagt catgtcatgg   1020

gaaggtatat tcaaagaagt tgatgatgag tttgagatgc aaaaatggtc aatggaattg   1080

gatcacgggc ccgtaggttg tgcggatagc ccaactattg attacccgtt aatcaatgtc   1140

gaaacgggct ctaaagaaag tgagattgac atgaattcta aaccggttaa cccggttccg   1200

actgaagaaa cggcggttaa cgatgttttt tgggagcagt tcttgaccga gaacccgggc   1260

gggtcaacgg gtaataatgt aggcgaaatg gtgagtgaag atagaaaata tgggaagttt   1320

tggtggaata tgagtagtgt aaataatctt gctgatcagc taggacagct tacttcagta   1380

gagagaagtt gataatggtg gattgtctaa atttactatt aatctgttat ttgtttaata   1440

ttggtggggt ggttgtaaat ttggtatgag ggtttatatg atttttgagc gttttgtaga   1500

taaaaaaaaa aaaaaa                                                   1516


<210>  4
<211>  387
<212>  PRT
<213>  Helianthus annuus

<400>  4

Met Met Asn Asp Val His Gly Asn Leu Asn Ser Asn Ser Asn Ser Gly 
1               5                   10                  15      


Ser Asn Ala Leu Pro Pro Phe Ile Ala Lys Thr Tyr Glu Met Val Asp 
            20                  25                  30          


Asp Pro Ser Thr Asn Ser Ile Val Ser Trp Ser Gln Thr Asn Arg Ser 
        35                  40                  45              


Phe Ile Val Trp Asn Pro Pro Glu Phe Ser Gly Glu Leu Leu Pro Arg 
    50                  55                  60                  


Phe Phe Lys His Asn Asn Phe Ser Ser Phe Ile Arg Gln Leu Asn Thr 
65                  70                  75                  80  


Tyr Ser Phe Arg Lys Ile Asp Pro Glu Leu Trp Glu Phe Ala Asn Glu 
                85                  90                  95      


Asp Phe Ile Arg Gly Gln Pro His Leu Leu Lys Asn Ile His Arg Arg 
            100                 105                 110         


Lys Pro Val His Ser His Ser Ile His Asn Leu Ala Asn Asn Gly Ser 
        115                 120                 125             


Ser Ser Ser Ser Ser Pro Leu Thr Glu Ser Glu Lys Gln Gln Tyr Ile 
    130                 135                 140                 


Glu Lys Ile Ser Thr Leu Gln His Glu Lys Glu Ser Leu Ser Ile Gln 
145                 150                 155                 160 


Phe His Met His Lys Gln Glu Gln Glu Arg Ile Lys Phe Glu Ala His 
                165                 170                 175     


Gly Leu Thr Asp Arg Leu Lys Arg Ala Ala Lys Leu Gln Lys Asp Ile 
            180                 185                 190         


Leu Cys Cys Leu Asp Glu Phe Leu Gln Lys Pro Pro Gln Glu Cys Glu 
        195                 200                 205             


Phe Thr Pro Arg Leu Met Glu Asn Arg Lys Arg Arg Leu Ser Ser Glu 
    210                 215                 220                 


Asn Val Asn Glu Gln Val Cys Pro Phe Asp Ile Pro Ile Arg Glu Thr 
225                 230                 235                 240 


Ile Thr Ala Asp Ala Leu Leu Ala Leu Asn Thr Glu Leu Val Glu Gln 
                245                 250                 255     


Leu Glu Ser Ser Val Met Ser Trp Glu Gly Ile Phe Lys Glu Val Asp 
            260                 265                 270         


Asp Glu Phe Glu Met Gln Lys Trp Ser Met Glu Leu Asp His Gly Pro 
        275                 280                 285             


Val Gly Cys Ala Asp Ser Pro Thr Ile Asp Tyr Pro Leu Ile Asn Val 
    290                 295                 300                 


Glu Thr Gly Ser Lys Glu Ser Glu Ile Asp Met Asn Ser Lys Pro Val 
305                 310                 315                 320 


Asn Pro Val Pro Thr Glu Glu Thr Ala Val Asn Asp Val Phe Trp Glu 
                325                 330                 335     


Gln Phe Leu Thr Glu Asn Pro Gly Gly Ser Thr Gly Asn Asn Val Gly 
            340                 345                 350         


Glu Met Val Ser Glu Asp Arg Lys Tyr Gly Lys Phe Trp Trp Asn Met 
        355                 360                 365             


Ser Ser Val Asn Asn Leu Ala Asp Gln Leu Gly Gln Leu Thr Ser Val 
    370                 375                 380                 


Glu Arg Ser 
385         


<210>  5
<211>  94
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  DBD of HaHSFA9

<400>  5

Pro Pro Pro Phe Leu Lys Lys Thr Phe Glu Met Val Asp Asp Pro Thr 
1               5                   10                  15      


Thr Asp Ser Ile Ile Ser Trp Ser Ser Ser Lys Asn Ser Phe Ile Leu 
            20                  25                  30          


Trp Asp Pro His Lys Phe Ser Thr Asp Leu Leu Pro Gln Arg Phe Lys 
        35                  40                  45              


His Asn Asn Phe Ser Ser Phe Val Arg Gln Leu Asn Thr Tyr Arg Phe 
    50                  55                  60                  


Lys Lys Ile Asp Pro Asp Arg Trp Glu Phe Ala Asn Glu Phe Phe Gln 
65                  70                  75                  80  


Lys Gly Lys Lys His Leu Leu Arg Asp Ile Lys Arg Arg Thr 
                85                  90                  


<210>  6
<211>  22
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  Motifs in the OD of HaHSFA9 (HR-A)

<400>  6

Met Glu Thr Glu Leu Lys Asn Leu Arg Lys Glu Arg Ile Thr Leu Lys 
1               5                   10                  15      


Gln Glu Ile Leu Lys Met 
            20          


<210>  7
<211>  8
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  Motifs in the OD of HaHSFA9 (HR-B)

<400>  7

Leu Leu Val Phe Met Ser Lys Ala 
1               5               


<210>  8
<211>  26
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  Motifs in the OD of HaHSFA9 (bipartite NLS)


<220>
<221>  REPEAT
<222>  (16)..(16)
<223>  The aa at position 16 represented by a Xaa, is a repeat site of 1
       to 12 Xaa amino acids.  Where Xaa is any amino acid.

<400>  8

Gln Lys Gln Lys Thr Gly Ser Val Glu Met Cys Lys Lys Arg Lys Xaa 
1               5                   10                  15      


Phe Gln Glu Met Trp Asn Met Ile Glu Pro 
            20                  25      


<210>  9
<211>  10
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  Motifs in the OD of HaHSFA9 (AHA1)

<400>  9

Phe Ile Leu Trp Glu Lys Leu Met Glu Asp 
1               5                   10  


<210>  10
<211>  9
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  Motifs in the OD of HaHSFA9 (AHA2)

<400>  10

Leu Gln Glu Trp Glu Glu Leu Ile Pro 
1               5                   


<210>  11
<211>  93
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  HaHSFA4 DBD (aa 21-113)

<400>  11

Pro Pro Phe Ile Ala Lys Thr Tyr Glu Met Val Asp Asp Pro Ser Thr 
1               5                   10                  15      


Asn Ser Ile Val Ser Trp Ser Gln Thr Asn Arg Ser Phe Ile Val Trp 
            20                  25                  30          


Asn Pro Pro Glu Phe Ser Gly Glu Leu Leu Pro Arg Phe Phe Lys His 
        35                  40                  45              


Asn Asn Phe Ser Ser Phe Ile Arg Gln Leu Asn Thr Tyr Ser Phe Arg 
    50                  55                  60                  


Lys Ile Asp Pro Glu Leu Trp Glu Phe Ala Asn Glu Asp Phe Ile Arg 
65                  70                  75                  80  


Gly Gln Pro His Leu Leu Lys Asn Ile His Arg Arg Lys 
                85                  90              


<210>  12
<211>  57
<212>  PRT
<213>  AHelianthus annuus

<220>
<223>  HaHSFA4 OD (aa 143-199):

<400>  12

Tyr Ile Glu Lys Ile Ser Thr Leu Gln His Glu Lys Glu Ser Leu Ser 
1               5                   10                  15      


Ile Gln Phe His Met His Lys Gln Glu Gln Glu Arg Ile Lys Phe Glu 
            20                  25                  30          


Ala His Gly Leu Thr Asp Arg Leu Lys Arg Ala Ala Lys Leu Gln Lys 
        35                  40                  45              


Asp Ile Leu Cys Cys Leu Asp Glu Phe 
    50                  55          


<210>  13
<211>  7
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  HaHSFA4 NLS (aa 216-222)

<400>  13

Asn Arg Lys Arg Arg Leu Ser 
1               5           


<210>  14
<211>  10
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  HaHSFA4 AHA1 (aa 261-270)

<400>  14

Val Met Ser Trp Glu Gly Ile Phe Lys Glu 
1               5                   10  


<210>  15
<211>  10
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  HaHSFA4 AHA2 (aa 332-341)

<400>  15

Asp Val Phe Trp Glu Gln Phe Leu Thr Glu 
1               5                   10  


<210>  16
<211>  8
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  HaHSFA4 NES (aa 374-381)

<400>  16

Leu Ala Asp Gln Leu Gly Gln Leu 
1               5               


<210>  17
<211>  28
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  HaHSFA9 LNK (aa 163 - 190)

<400>  17

Asn Gln Pro Gln Asn Thr Gln Lys Gln Glu Glu Ile Arg Lys Gln Glu 
1               5                   10                  15      


Gln Gln Gln Cys Cys Gly His Gln Thr Asn Ser Thr 
            20                  25              


<210>  18
<211>  124
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  HaHSFA9 CTAD (aa 248 - 371)

<400>  18

Phe Arg Asn Pro Ile Phe Val Lys Leu Leu Gln His Leu Val Gln Lys 
1               5                   10                  15      


Gln Lys Thr Gly Ser Val Glu Met Cys Lys Lys Arg Lys Leu Glu Gln 
            20                  25                  30          


Met Leu Asn Thr Asp Asp Leu Asp Arg Phe Gln Glu Met Trp Asn Met 
        35                  40                  45              


Ile Glu Pro Asp Ala Tyr Thr Val Leu Ser Ser Asp Gly Ser Val Ser 
    50                  55                  60                  


Pro Pro Glu Asp Gln Asn Thr Ser Asp Lys Ser Gly Ser Asn Gly Ser 
65                  70                  75                  80  


Asp Tyr Asn Ser Glu Ser Phe Ile Leu Trp Glu Lys Leu Met Glu Asp 
                85                  90                  95      


Glu Leu Ile Phe Gly Gly Glu Gln Ser Gly Lys Asp Gln Thr Glu Thr 
            100                 105                 110         


Tyr Leu Gln Glu Trp Glu Glu Leu Ile Pro Lys Ala 
        115                 120                 


<210>  19
<211>  10
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  HaHSFA9 CTAD AHA motif

<400>  19

Phe Gln Glu Met Trp Asn Met Ile Glu Pro 
1               5                   10  


<210>  20
<211>  10
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  HaHSFA9 CTAD AHA motif

<400>  20

Phe Ile Leu Trp Glu Lys Leu Met Glu Asp 
1               5                   10  


<210>  21
<211>  8
<212>  PRT
<213>  Helianthus annuus

<220>
<223>  HaHSFA9 CTAD AHA motif

<400>  21

Gln Glu Trp Glu Glu Leu Ile Pro 
1               5               


<210>  22
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  22
catctcttca gacaaat                                                    17


<210>  23
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  23
actatccttc gcaagaccct tcc                                             23


<210>  24
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  24
catctcttca gacaaat                                                    17


<210>  25
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  25
ccaccacgtc atcataccac                                                 20


<210>  26
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  HSFA4-RACE Primer

<400>  26
ggagacgacg acgatgatga tccatt                                          26


<210>  27
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  27
aaacgaattc gcctctagac actgacatgg actga                                35


<210>  28
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  28
tgatccattg tttgcaagat t                                               21


<210>  29
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide

<400>  29
ggtatatctt ggtccatggt gaatgatgtt catgggaatt tg                        42


<210>  30
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Oligonucleotide

<400>  30
gagctcggta cccggggatc tgcag                                           25


<210>  31
<211>  49
<212>  DNA
<213>  Artificial

<220>
<223>  Mutagenic Primer

<400>  31
tgtaaataat gctgctgatc aggcaggaca ggctacttca gtagagaga                 49


<210>  32
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer (OliA)

<400>  32
actatccttc gcaagaccct tcc                                             23


<210>  33
<211>  46
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer (OliB)

<400>  33
gtttttgtgt gttttgtggc tggcaagcac cagatccttg ttgatt                    46


<210>  34
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer (OliC)

<400>  34
cagccacaaa acacacaaaa ac                                              22


<210>  35
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer (OliD)

<400>  35
tcggaattaa ccctcactaa ag                                              22


<210>  36
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer (OliD')

<400>  36
tctagaacta gtggatc                                                    17


<210>  37
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  37
gttgttggta tatctagatc aatgatgaat gatgttcatg g                         41


<210>  38
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  38
gtaaatttag acagtcgacc attatcaact tctctctact g                         41


<210>  39
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  39
tctagtaaaa atggcatacc                                                 20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  40
ttatcaactt ctctctactg                                                 20


