                         SEQUENCE LISTING

<110>  FUNDACI PRIVADA CENTRE DE REGULACI GENMICA (CRG)
       INSTITUCI CATALANA DE RECERCA I ESTUDIS AVANATS (ICREA)
       THE REGENTS OF THE UNIVERSITY OF MICHIGAN
 
<120>  Means and methods for the treatment of synucleinopathies

<130>  BAR/PARK/481

<150>  ES P201232071
<151>  2012-12-31

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  1233
<212>  DNA
<213>  Homo sapiens

<400>  1
atggtgtgct tccgcctctt cccggttccg ggctcagggc tcgttctggt ctgcctagtc       60

ctgggagctg tgcggtctta tgcattggaa cttaatttga cagattcaga aaatgccact      120

tgcctttatg caaaatggca gatgaatttc acagtacgct atgaaactac aaataaaact      180

tataaaactg taaccatttc agaccatggc actgtgacat ataatggaag catttgtggg      240

gatgatcaga atggtcccaa aatagcagtg cagttcggat ctggcttttc ctggattgcg      300

aattttacca aggcagcatc tacttattca attgacagcg tctcattttc ctacaacact      360

ggtgataaca caacatttcc tgatgctgaa gataaaggaa ttcttactgt tgatgaactt      420

ttggccatca gaattccatt gaatgacctt tttagatgca atagtttatc aactttggaa      480

aagaatgatg ttgtccaaca ctactgggat gttcttgtac aagcttttgt ccaaaatggc      540

acagtgagca caaatgagtt cctgtgtgat aaagacaaaa cttcaacagt ggcacccacc      600

atacacacca ctgtgccatc tcctactaca acacctactc caaaggaaaa accagaagct      660

ggaacctatt cagttaataa tggcaatgat acttgtctgc tggctaccat ggggctgcag      720

ctgaacatca ctcaggataa ggttgcttca gttattaaca tcaaccccaa tacaactcac      780

tccacaggca gctgccgttc tcacactgct ctacttagac tcaatagcag caccattaag      840

tatctagact ttgtctttgc tgtgaaaaat gaaaaccgat tttatctgaa ggaagtgaac      900

atcagcatgt atttggttaa tggctccgtt ttcagcattg caaataacaa tctcagctac      960

tgggatgccc ccctgggaag ttcttatatg tgcaacaaag agcagactgt ttcagtgtct     1020

ggagcatttc agataaatac ctttgatcta agggttcagc ctttcaatgt gacacaagga     1080

aagtattcta cagctcaaga ctgcagtgca gatgacgaca acttccttgt gcccatagcg     1140

gtgggagctg ccttggcagg agtacttatt ctagtgttgc tggcttattt tattggtctc     1200

aagcaccatc atgctggata tgagcaattt tag                                  1233


<210>  2
<211>  410
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Val Cys Phe Arg Leu Phe Pro Val Pro Gly Ser Gly Leu Val Leu 
1               5                   10                  15      


Val Cys Leu Val Leu Gly Ala Val Arg Ser Tyr Ala Leu Glu Leu Asn 
            20                  25                  30          


Leu Thr Asp Ser Glu Asn Ala Thr Cys Leu Tyr Ala Lys Trp Gln Met 
        35                  40                  45              


Asn Phe Thr Val Arg Tyr Glu Thr Thr Asn Lys Thr Tyr Lys Thr Val 
    50                  55                  60                  


Thr Ile Ser Asp His Gly Thr Val Thr Tyr Asn Gly Ser Ile Cys Gly 
65                  70                  75                  80  


Asp Asp Gln Asn Gly Pro Lys Ile Ala Val Gln Phe Gly Ser Gly Phe 
                85                  90                  95      


Ser Trp Ile Ala Asn Phe Thr Lys Ala Ala Ser Thr Tyr Ser Ile Asp 
            100                 105                 110         


Ser Val Ser Phe Ser Tyr Asn Thr Gly Asp Asn Thr Thr Phe Pro Asp 
        115                 120                 125             


Ala Glu Asp Lys Gly Ile Leu Thr Val Asp Glu Leu Leu Ala Ile Arg 
    130                 135                 140                 


Ile Pro Leu Asn Asp Leu Phe Arg Cys Asn Ser Leu Ser Thr Leu Glu 
145                 150                 155                 160 


Lys Asn Asp Val Val Gln His Tyr Trp Asp Val Leu Val Gln Ala Phe 
                165                 170                 175     


Val Gln Asn Gly Thr Val Ser Thr Asn Glu Phe Leu Cys Asp Lys Asp 
            180                 185                 190         


Lys Thr Ser Thr Val Ala Pro Thr Ile His Thr Thr Val Pro Ser Pro 
        195                 200                 205             


Thr Thr Thr Pro Thr Pro Lys Glu Lys Pro Glu Ala Gly Thr Tyr Ser 
    210                 215                 220                 


Val Asn Asn Gly Asn Asp Thr Cys Leu Leu Ala Thr Met Gly Leu Gln 
225                 230                 235                 240 


Leu Asn Ile Thr Gln Asp Lys Val Ala Ser Val Ile Asn Ile Asn Pro 
                245                 250                 255     


Asn Thr Thr His Ser Thr Gly Ser Cys Arg Ser His Thr Ala Leu Leu 
            260                 265                 270         


Arg Leu Asn Ser Ser Thr Ile Lys Tyr Leu Asp Phe Val Phe Ala Val 
        275                 280                 285             


Lys Asn Glu Asn Arg Phe Tyr Leu Lys Glu Val Asn Ile Ser Met Tyr 
    290                 295                 300                 


Leu Val Asn Gly Ser Val Phe Ser Ile Ala Asn Asn Asn Leu Ser Tyr 
305                 310                 315                 320 


Trp Asp Ala Pro Leu Gly Ser Ser Tyr Met Cys Asn Lys Glu Gln Thr 
                325                 330                 335     


Val Ser Val Ser Gly Ala Phe Gln Ile Asn Thr Phe Asp Leu Arg Val 
            340                 345                 350         


Gln Pro Phe Asn Val Thr Gln Gly Lys Tyr Ser Thr Ala Gln Asp Cys 
        355                 360                 365             


Ser Ala Asp Asp Asp Asn Phe Leu Val Pro Ile Ala Val Gly Ala Ala 
    370                 375                 380                 


Leu Ala Gly Val Leu Ile Leu Val Leu Leu Ala Tyr Phe Ile Gly Leu 
385                 390                 395                 400 


Lys His His His Ala Gly Tyr Glu Gln Phe 
                405                 410 


<210>  3
<211>  1233
<212>  DNA
<213>  Homo sapiens

<400>  3
atggtgtgct tccgcctctt cccggttccg ggctcagggc tcgttctggt ctgcctagtc       60

ctgggagctg tgcggtctta tgcattggaa cttaatttga cagattcaga aaatgccact      120

tgcctttatg caaaatggca gatgaatttc acagtacgct atgaaactac aaataaaact      180

tataaaactg taaccatttc agaccatggc actgtgacat ataatggaag catttgtggg      240

gatgatcaga atggtcccaa aatagcagtg cagttcggat tcggcttttc ctggattgcg      300

aattttacca aggcagcatc tacttattca attgacagcg tctcattttc ctacaacact      360

ggtgataaca caacatttcc tgatgctgaa gataaaggaa ttcttactgt tgatgaactt      420

ttggccatca gaattccatt gaatgacctt tttagatgca atagtttatc aactttggaa      480

aagaatgatg ttgtccaaca ctactgggat gttcttgtac aagcttttgt ccaaaatggc      540

acagtgagca caaatgagtt cctgtgtgat aaagacaaaa cttcaacagt ggcacccacc      600

atacacacca ctgtgccatc tcctactaca acacctactc caaaggaaaa accagaagct      660

ggaacctatt cagttaataa tggcaatgat acttgtctgc tggctaccat ggggctgcag      720

ctgaacatca ctcaggataa ggttgcttca gttattaaca tcaaccccaa tacaactcac      780

tccacaggca gctgccgttc tcacactgct ctacttagac tcaatagcag caccattaag      840

tatctagact ttgtctttgc tgtgaaaaat gaaaaccgat tttatctgaa ggaagtgaac      900

atcagcatgt atttggttaa tggctccgtt ttcagcattg caaataacaa tctcagctac      960

tgggatgccc ccctgggaag ttcttatatg tgcaacaaag agcagactgt ttcagtgtct     1020

ggagcatttc agataaatac ctttgatcta agggttcagc ctttcaatgt gacacaagga     1080

aagtattcta cagctcaaga ctgcagtgca gatgacgaca acttccttgt gcccatagcg     1140

gtgggagctg ccttggcagg agtacttatt ctagtgttgc tggcttattt tattggtctc     1200

aagcaccatc atgctggata tgagcaattt tag                                  1233


<210>  4
<211>  410
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Val Cys Phe Arg Leu Phe Pro Val Pro Gly Ser Gly Leu Val Leu 
1               5                   10                  15      


Val Cys Leu Val Leu Gly Ala Val Arg Ser Tyr Ala Leu Glu Leu Asn 
            20                  25                  30          


Leu Thr Asp Ser Glu Asn Ala Thr Cys Leu Tyr Ala Lys Trp Gln Met 
        35                  40                  45              


Asn Phe Thr Val Arg Tyr Glu Thr Thr Asn Lys Thr Tyr Lys Thr Val 
    50                  55                  60                  


Thr Ile Ser Asp His Gly Thr Val Thr Tyr Asn Gly Ser Ile Cys Gly 
65                  70                  75                  80  


Asp Asp Gln Asn Gly Pro Lys Ile Ala Val Gln Phe Gly Phe Gly Phe 
                85                  90                  95      


Ser Trp Ile Ala Asn Phe Thr Lys Ala Ala Ser Thr Tyr Ser Ile Asp 
            100                 105                 110         


Ser Val Ser Phe Ser Tyr Asn Thr Gly Asp Asn Thr Thr Phe Pro Asp 
        115                 120                 125             


Ala Glu Asp Lys Gly Ile Leu Thr Val Asp Glu Leu Leu Ala Ile Arg 
    130                 135                 140                 


Ile Pro Leu Asn Asp Leu Phe Arg Cys Asn Ser Leu Ser Thr Leu Glu 
145                 150                 155                 160 


Lys Asn Asp Val Val Gln His Tyr Trp Asp Val Leu Val Gln Ala Phe 
                165                 170                 175     


Val Gln Asn Gly Thr Val Ser Thr Asn Glu Phe Leu Cys Asp Lys Asp 
            180                 185                 190         


Lys Thr Ser Thr Val Ala Pro Thr Ile His Thr Thr Val Pro Ser Pro 
        195                 200                 205             


Thr Thr Thr Pro Thr Pro Lys Glu Lys Pro Glu Ala Gly Thr Tyr Ser 
    210                 215                 220                 


Val Asn Asn Gly Asn Asp Thr Cys Leu Leu Ala Thr Met Gly Leu Gln 
225                 230                 235                 240 


Leu Asn Ile Thr Gln Asp Lys Val Ala Ser Val Ile Asn Ile Asn Pro 
                245                 250                 255     


Asn Thr Thr His Ser Thr Gly Ser Cys Arg Ser His Thr Ala Leu Leu 
            260                 265                 270         


Arg Leu Asn Ser Ser Thr Ile Lys Tyr Leu Asp Phe Val Phe Ala Val 
        275                 280                 285             


Lys Asn Glu Asn Arg Phe Tyr Leu Lys Glu Val Asn Ile Ser Met Tyr 
    290                 295                 300                 


Leu Val Asn Gly Ser Val Phe Ser Ile Ala Asn Asn Asn Leu Ser Tyr 
305                 310                 315                 320 


Trp Asp Ala Pro Leu Gly Ser Ser Tyr Met Cys Asn Lys Glu Gln Thr 
                325                 330                 335     


Val Ser Val Ser Gly Ala Phe Gln Ile Asn Thr Phe Asp Leu Arg Val 
            340                 345                 350         


Gln Pro Phe Asn Val Thr Gln Gly Lys Tyr Ser Thr Ala Gln Asp Cys 
        355                 360                 365             


Ser Ala Asp Asp Asp Asn Phe Leu Val Pro Ile Ala Val Gly Ala Ala 
    370                 375                 380                 


Leu Ala Gly Val Leu Ile Leu Val Leu Leu Ala Tyr Phe Ile Gly Leu 
385                 390                 395                 400 


Lys His His His Ala Gly Tyr Glu Gln Phe 
                405                 410 


<210>  5
<211>  1248
<212>  DNA
<213>  Mus musculus

<400>  5
atgtgcctct ctccggttaa aggcgcaaag ctcatcctga tctttctgtt cctaggagcc       60

gttcagtcca atgcattgat agttaatttg acagattcaa agggtacttg cctttatgca      120

gaatgggaga tgaatttcac aataacatat gaaactacaa accaaaccaa taaaactata      180

accattgcag tacctgacaa ggcgacacac gatggaagca gttgtgggga tgaccggaat      240

agtgccaaaa taatgataca atttggaccc gctgtctctt gggctgtgaa ttttaccaag      300

gaagcatctc attattcaat tcatgacatc gtgctttcct acaacactag tgatagcaca      360

gtatttcctg gtgctgtagc taaaggagtt catactgtta aaaatcctga gaatttcaaa      420

gttccattgg atgtcatctt taagtgcaat agtgttttaa cttacaacct gactcctgtc      480

gttcagaaat attggggtat tcacctgcaa gcttttgtcc aaaatggtac agtgagtaaa      540

aatgaacaag tgtgtgaaga agaccaaact cccaccactg tggcacccat cattcacacc      600

actgccccgt cgactacaac tacactcact ccaacttcaa cacccactcc aactccaact      660

ccaactccaa ccgttggaaa ctacagcatt agaaatggca atactacctg tctgctggct      720

accatggggc tgcagctgaa catcactgag gagaaggtgc ctttcatttt taacatcaac      780

cctgccacaa ccaacttcac cggcagctgt caacctcaaa gtgctcaact taggctgaac      840

aacagccaaa ttaagtatct tgactttatc tttgctgtga aaaatgaaaa acggttctat      900

ctgaaggaag tgaatgtcta catgtatttg gctaatggct cagctttcaa catttccaac      960

aagaacctta gcttctggga tgcccctctg ggaagttctt atatgtgcaa caaagagcag     1020

gtgctttctg tgtctagagc gtttcagatc aacaccttta acctaaaggt gcaacctttt     1080

aatgtgacaa aaggacagta ttctacagct caagactgca gtgcagatga agacaacttc     1140

cttgtgccca tagcggtggg agcagctctg ggaggagtac ttattctagt gttgctggct     1200

tattttattg gtctcaagcg ccatcatact ggatatgagc aattttag                  1248


<210>  6
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
atggtgtgct tccgcctctt cccgg                                             25


<210>  7
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
ctaaaattgc tcatatccag catga                                             25


<210>  8
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  LAMP2A siRNA

<400>  8
uuucccuacu cucuuucuc                                                    19


<210>  9
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  control, scrambled siRNA

<400>  9
gcgacguucc ugaaaccac                                                    19


