                         SEQUENCE LISTING

<110>  Johns Hopkins Technology Transfer
 
<120>  PROTECTION FROM OXIDATIVE DAMAGE BY GENE TRANSFER OF BY GLUTAMATE
        CYSTEINE LIGASE AND GLUTATHIONE SYNTHASE

<130>  P12041-02

<150>  61/739,542
<151>  2012-12-19

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  825
<212>  DNA
<213>  Homo sapiens

<400>  1
atgggcaccg acagccgcgc ggccaaggcg ctcctggcgc gggcccgcac cctgcacctg       60

cagacgggga acctgctgaa ctggggccgc ctgcggaaga agtgcccgtc cacgcacagc      120

gaggagcttc atgattgtat ccaaaaaacc ttgaatgaat ggagttccca aatcaaccca      180

gatttggtca gggagtttcc agatgtcttg gaatgcactg tatctcatgc agtagaaaag      240

ataaatcctg atgaaagaga agaaatgaaa gtttctgcaa aactgttcat tgtagaatca      300

aactcttcat catcaactag aagtgcagtt gacatggcct gttcagtcct tggagttgca      360

cagctggatt ctgtgatcat tgcttcacct cctattgaag atggagttaa tctttccttg      420

gagcatttac agccttactg ggaggaatta gaaaacttag ttcagagcaa aaagattgtt      480

gccataggta cctctgatct agacaaaaca cagttggaac agctgtatca gtgggcacag      540

gtaaaaccaa atagtaacca agttaatctt gcctcctgct gtgtgatgcc accagatttg      600

actgcatttg ctaaacaatt tgacatacag ctgttgactc acaatgatcc aaaagaactg      660

ctttctgaag caagtttcca agaagctctt caggaaagca ttcctgacat tcaagcgcac      720

gagtgggtgc cgctgtggct actgcggtat tcggtcattg tgaaaagtag aggaattatc      780

aaatcaaaag gctacatttt acaagctaaa agaaggggtt cttaa                      825


<210>  2
<211>  1914
<212>  DNA
<213>  Homo sapiens

<400>  2
atggggctgc tgtcccaggg ctcgccgctg agctgggagg aaaccaagcg ccatgccgac       60

cacgtgcggc ggcacgggat cctccagttc ctgcacatct accacgccgt caaggaccgg      120

cacaaggacg ttctcaagtg gggcgatgag gtggaataca tgttggtatc ttttgatcat      180

gaaaataaaa aagtccggtt ggtcctgtct ggggagaaag ttcttgaaac tctgcaagag      240

aagggggaaa ggacaaaccc aaaccatcct accctttgga gaccagagta tgggagttac      300

atgattgaag ggacaccagg acagccctac ggaggaacaa tgtccgagtt caatacagtt      360

gaggccaaca tgcgaaaacg ccggaaggag gctacttcta tattagaaga aaatcaggct      420

ctttgcacaa taacttcatt tcccagatta ggctgtcctg ggttcacact gcccgaggtc      480

aaacccaacc cagtggaagg aggagcttcc aagtccctct tctttccaga tgaagcaata      540

aacaagcacc ctcgcttcag taccttaaca agaaatatcc gacataggag aggagaaaag      600

gttgtcatca atgtaccaat atttaaggac aagaatacac catctccatt tatagaaaca      660

tttactgagg atgatgaagc ttcaagggct tctaagccgg atcatattta catggatgcc      720

atgggatttg gaatgggcaa ttgctgtctc caggtgacat tccaagcctg cagtatatct      780

gaggccagat acctttatga tcagttggct actatctgtc caattgttat ggctttgagt      840

gctgcatctc ccttttaccg aggctatgtg tcagacattg attgtcgctg gggagtgatt      900

tctgcatctg tagatgatag aactcgggag gagcgaggac tggagccatt gaagaacaat      960

aactatagga tcagtaaatc ccgatatgac tcaatagaca gctatttatc taagtgtggt     1020

gagaaatata atgacatcga cttgacgata gataaagaga tctacgaaca gctgttgcag     1080

gaaggcattg atcatctcct ggcccagcat gttgctcatc tctttattag agacccactg     1140

acactgtttg aagagaaaat acacctggat gatgctaatg agtctgacca ttttgagaat     1200

attcagtcca caaattggca gacaatgaga tttaagcccc ctcctccaaa ctcagacatt     1260

ggatggagag tagaatttcg acccatggag gtgcaattaa cagactttga gaactctgcc     1320

tatgtggtgt ttgtggtact gctcaccaga gtgatccttt cctacaaatt ggattttctc     1380

attccactgt caaaggttga tgagaacatg aaggtagcac agaaaagaga tgctgtcttg     1440

cagggaatgt tttatttcag gaaagatatt tgcaaaggtg gcaatgcagt ggtggatggt     1500

tgtggcaagg cccagaacag cacggagctc gctgcagagg agtacaccct catgagcata     1560

gacaccatca tcaatgggaa ggaaggtgtg tttcctggac tgatcccaat tctgaactct     1620

taccttgaaa acatggaagt ggatgtggac accagatgta gtattctgaa ctacctaaag     1680

ctaattaaga agagagcatc tggagaacta atgacagttg ccagatggat gagggagttt     1740

atcgcaaacc atcctgacta caagcaagac agtgtcataa ctgatgaaat gaattatagc     1800

cttattttga agtgtaacca aattgcaaat gaattatgtg aatgcccaga gttacttgga     1860

tcagcattta ggaaagtaaa atatagtgga agtaaaactg actcatccaa ctag           1914


<210>  3
<211>  1425
<212>  DNA
<213>  Homo sapiens

<400>  3
atggccacca actgggggag cctcttgcag gataaacagc agctagagga gctggcacgg       60

caggccgtgg accgggccct ggctgaggga gtattgctga ggacctcaca ggagcccact      120

tcctcggagg tggtgagcta tgccccattc acgctcttcc cctcactggt ccccagtgcc      180

ctgctggagc aagcctatgc tgtgcagatg gacttcaacc tgctagtgga tgctgtcagc      240

cagaacgctg ccttcctgga gcaaactctt tccagcacca tcaaacagga tgactttacc      300

gctcgtctct ttgacatcca caagcaagtc ctaaaagagg gcattgccca gactgtgttc      360

ctgggcctga atcgctcaga ctacatgttc cagcgcagcg cagatggctc cccagccctg      420

aaacagatcg aaatcaacac catctctgcc agctttgggg gcctggcctc ccggacccca      480

gctgtgcacc gacatgttct cagtgtcctg agtaagacca aagaagctgg caagatcctc      540

tctaataatc ccagcaaggg actggccctg ggaattgcca aagcctggga gctctacggc      600

tcacccaatg ctctggtgct actgattgct caagagaagg aaagaaacat atttgaccag      660

cgtgccatag agaatgagct actggccagg aacatccatg tgatccgacg aacatttgaa      720

gatatctctg aaaaggggtc tctggaccaa gaccgaaggc tgtttgtgga tggccaggaa      780

attgctgtgg tttacttccg ggatggctac atgcctcgtc agtacagtct acagaattgg      840

gaagcacgtc tactgctgga gaggtcacat gctgccaagt gcccagacat tgccacccag      900

ctggctggga ctaagaaggt gcagcaggag ctaagcaggc cgggcatgct ggagatgttg      960

ctccctggcc agcctgaggc tgtggcccgc ctccgcgcca cctttgctgg cctctactca     1020

ctggatgtgg gtgaagaagg ggaccaggcc atcgccgagg cccttgctgc ccctagccgg     1080

tttgtgctaa agccccagag agagggtgga ggtaacaacc tatatgggga ggaaatggta     1140

caggccctga aacagctgaa ggacagtgag gagagggcct cctacatcct catggagaag     1200

atcgaacctg agccttttga gaattgcctg ctacggcctg gcagccctgc ccgagtggtc     1260

cagtgcattt cagagctggg catctttggg gtctatgtca ggcaggaaaa gacactcgtg     1320

atgaacaagc acgtggggca tctacttcga accaaagcca tcgagcatgc agatggtggt     1380

gtggcagcgg gagtggcagt cctggacaac ccataccctg tgtga                     1425


<210>  4
<211>  29
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Met Ser Phe Val Ser Leu Leu Leu Val Gly Ile Leu Phe Trp Ala 
1               5                   10                  15      


Thr Glu Ala Glu Gln Leu Thr Lys Cys Glu Val Phe Gln 
            20                  25                  


<210>  5
<211>  4
<212>  PRT
<213>  Homo sapiens

<400>  5

Lys Asp Glu Leu 
1               


<210>  6
<211>  25
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Leu Ser Leu Arg Gln Ser Ile Arg Phe Phe Lys Pro Ala Thr Arg 
1               5                   10                  15      


Thr Leu Cys Ser Ser Arg Tyr Leu Leu 
            20                  25  


<210>  7
<211>  35
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Leu Phe Asn Leu Arg Ile Leu Leu Asn Asn Ala Ala Phe Arg Asn 
1               5                   10                  15      


Gly His Asn Phe Met Val Arg Asn Phe Arg Cys Gly Gln Pro Leu Gln 
            20                  25                  30          


Leu Gly Ser 
        35  


<210>  8
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Val Leu Pro Arg Leu Tyr Thr Ala Thr Ser Arg Ala Ala 
1               5                   10                  


<210>  9
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Val Leu Pro Arg Leu Tyr Thr Ala Thr Ser Arg Ala Ala 
1               5                   10                  


<210>  10
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  10

Pro Pro Lys Lys Lys Arg Lys Val 
1               5               


<210>  11
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  11

Gly Ser Ser Lys Ser Lys Pro Lys 
1               5               


<210>  12
<211>  274
<212>  PRT
<213>  Homo sapiens

<400>  12

Met Gly Thr Asp Ser Arg Ala Ala Lys Ala Leu Leu Ala Arg Ala Arg 
1               5                   10                  15      


Thr Leu His Leu Gln Thr Gly Asn Leu Leu Asn Trp Gly Arg Leu Arg 
            20                  25                  30          


Lys Lys Cys Pro Ser Thr His Ser Glu Glu Leu His Asp Cys Ile Gln 
        35                  40                  45              


Lys Thr Leu Asn Glu Trp Ser Ser Gln Ile Asn Pro Asp Leu Val Arg 
    50                  55                  60                  


Glu Phe Pro Asp Val Leu Glu Cys Thr Val Ser His Ala Val Glu Lys 
65                  70                  75                  80  


Ile Asn Pro Asp Glu Arg Glu Glu Met Lys Val Ser Ala Lys Leu Phe 
                85                  90                  95      


Ile Val Glu Ser Asn Ser Ser Ser Ser Thr Arg Ser Ala Val Asp Met 
            100                 105                 110         


Ala Cys Ser Val Leu Gly Val Ala Gln Leu Asp Ser Val Ile Ile Ala 
        115                 120                 125             


Ser Pro Pro Ile Glu Asp Gly Val Asn Leu Ser Leu Glu His Leu Gln 
    130                 135                 140                 


Pro Tyr Trp Glu Glu Leu Glu Asn Leu Val Gln Ser Lys Lys Ile Val 
145                 150                 155                 160 


Ala Ile Gly Thr Ser Asp Leu Asp Lys Thr Gln Leu Glu Gln Leu Tyr 
                165                 170                 175     


Gln Trp Ala Gln Val Lys Pro Asn Ser Asn Gln Val Asn Leu Ala Ser 
            180                 185                 190         


Cys Cys Val Met Pro Pro Asp Leu Thr Ala Phe Ala Lys Gln Phe Asp 
        195                 200                 205             


Ile Gln Leu Leu Thr His Asn Asp Pro Lys Glu Leu Leu Ser Glu Ala 
    210                 215                 220                 


Ser Phe Gln Glu Ala Leu Gln Glu Ser Ile Pro Asp Ile Gln Ala His 
225                 230                 235                 240 


Glu Trp Val Pro Leu Trp Leu Leu Arg Tyr Ser Val Ile Val Lys Ser 
                245                 250                 255     


Arg Gly Ile Ile Lys Ser Lys Gly Tyr Ile Leu Gln Ala Lys Arg Arg 
            260                 265                 270         


Gly Ser 
        


<210>  13
<211>  637
<212>  PRT
<213>  Homo sapiens

<400>  13

Met Gly Leu Leu Ser Gln Gly Ser Pro Leu Ser Trp Glu Glu Thr Lys 
1               5                   10                  15      


Arg His Ala Asp His Val Arg Arg His Gly Ile Leu Gln Phe Leu His 
            20                  25                  30          


Ile Tyr His Ala Val Lys Asp Arg His Lys Asp Val Leu Lys Trp Gly 
        35                  40                  45              


Asp Glu Val Glu Tyr Met Leu Val Ser Phe Asp His Glu Asn Lys Lys 
    50                  55                  60                  


Val Arg Leu Val Leu Ser Gly Glu Lys Val Leu Glu Thr Leu Gln Glu 
65                  70                  75                  80  


Lys Gly Glu Arg Thr Asn Pro Asn His Pro Thr Leu Trp Arg Pro Glu 
                85                  90                  95      


Tyr Gly Ser Tyr Met Ile Glu Gly Thr Pro Gly Gln Pro Tyr Gly Gly 
            100                 105                 110         


Thr Met Ser Glu Phe Asn Thr Val Glu Ala Asn Met Arg Lys Arg Arg 
        115                 120                 125             


Lys Glu Ala Thr Ser Ile Leu Glu Glu Asn Gln Ala Leu Cys Thr Ile 
    130                 135                 140                 


Thr Ser Phe Pro Arg Leu Gly Cys Pro Gly Phe Thr Leu Pro Glu Val 
145                 150                 155                 160 


Lys Pro Asn Pro Val Glu Gly Gly Ala Ser Lys Ser Leu Phe Phe Pro 
                165                 170                 175     


Asp Glu Ala Ile Asn Lys His Pro Arg Phe Ser Thr Leu Thr Arg Asn 
            180                 185                 190         


Ile Arg His Arg Arg Gly Glu Lys Val Val Ile Asn Val Pro Ile Phe 
        195                 200                 205             


Lys Asp Lys Asn Thr Pro Ser Pro Phe Ile Glu Thr Phe Thr Glu Asp 
    210                 215                 220                 


Asp Glu Ala Ser Arg Ala Ser Lys Pro Asp His Ile Tyr Met Asp Ala 
225                 230                 235                 240 


Met Gly Phe Gly Met Gly Asn Cys Cys Leu Gln Val Thr Phe Gln Ala 
                245                 250                 255     


Cys Ser Ile Ser Glu Ala Arg Tyr Leu Tyr Asp Gln Leu Ala Thr Ile 
            260                 265                 270         


Cys Pro Ile Val Met Ala Leu Ser Ala Ala Ser Pro Phe Tyr Arg Gly 
        275                 280                 285             


Tyr Val Ser Asp Ile Asp Cys Arg Trp Gly Val Ile Ser Ala Ser Val 
    290                 295                 300                 


Asp Asp Arg Thr Arg Glu Glu Arg Gly Leu Glu Pro Leu Lys Asn Asn 
305                 310                 315                 320 


Asn Tyr Arg Ile Ser Lys Ser Arg Tyr Asp Ser Ile Asp Ser Tyr Leu 
                325                 330                 335     


Ser Lys Cys Gly Glu Lys Tyr Asn Asp Ile Asp Leu Thr Ile Asp Lys 
            340                 345                 350         


Glu Ile Tyr Glu Gln Leu Leu Gln Glu Gly Ile Asp His Leu Leu Ala 
        355                 360                 365             


Gln His Val Ala His Leu Phe Ile Arg Asp Pro Leu Thr Leu Phe Glu 
    370                 375                 380                 


Glu Lys Ile His Leu Asp Asp Ala Asn Glu Ser Asp His Phe Glu Asn 
385                 390                 395                 400 


Ile Gln Ser Thr Asn Trp Gln Thr Met Arg Phe Lys Pro Pro Pro Pro 
                405                 410                 415     


Asn Ser Asp Ile Gly Trp Arg Val Glu Phe Arg Pro Met Glu Val Gln 
            420                 425                 430         


Leu Thr Asp Phe Glu Asn Ser Ala Tyr Val Val Phe Val Val Leu Leu 
        435                 440                 445             


Thr Arg Val Ile Leu Ser Tyr Lys Leu Asp Phe Leu Ile Pro Leu Ser 
    450                 455                 460                 


Lys Val Asp Glu Asn Met Lys Val Ala Gln Lys Arg Asp Ala Val Leu 
465                 470                 475                 480 


Gln Gly Met Phe Tyr Phe Arg Lys Asp Ile Cys Lys Gly Gly Asn Ala 
                485                 490                 495     


Val Val Asp Gly Cys Gly Lys Ala Gln Asn Ser Thr Glu Leu Ala Ala 
            500                 505                 510         


Glu Glu Tyr Thr Leu Met Ser Ile Asp Thr Ile Ile Asn Gly Lys Glu 
        515                 520                 525             


Gly Val Phe Pro Gly Leu Ile Pro Ile Leu Asn Ser Tyr Leu Glu Asn 
    530                 535                 540                 


Met Glu Val Asp Val Asp Thr Arg Cys Ser Ile Leu Asn Tyr Leu Lys 
545                 550                 555                 560 


Leu Ile Lys Lys Arg Ala Ser Gly Glu Leu Met Thr Val Ala Arg Trp 
                565                 570                 575     


Met Arg Glu Phe Ile Ala Asn His Pro Asp Tyr Lys Gln Asp Ser Val 
            580                 585                 590         


Ile Thr Asp Glu Met Asn Tyr Ser Leu Ile Leu Lys Cys Asn Gln Ile 
        595                 600                 605             


Ala Asn Glu Leu Cys Glu Cys Pro Glu Leu Leu Gly Ser Ala Phe Arg 
    610                 615                 620                 


Lys Val Lys Tyr Ser Gly Ser Lys Thr Asp Ser Ser Asn 
625                 630                 635         


<210>  14
<211>  474
<212>  PRT
<213>  Homo sapiens

<400>  14

Met Ala Thr Asn Trp Gly Ser Leu Leu Gln Asp Lys Gln Gln Leu Glu 
1               5                   10                  15      


Glu Leu Ala Arg Gln Ala Val Asp Arg Ala Leu Ala Glu Gly Val Leu 
            20                  25                  30          


Leu Arg Thr Ser Gln Glu Pro Thr Ser Ser Glu Val Val Ser Tyr Ala 
        35                  40                  45              


Pro Phe Thr Leu Phe Pro Ser Leu Val Pro Ser Ala Leu Leu Glu Gln 
    50                  55                  60                  


Ala Tyr Ala Val Gln Met Asp Phe Asn Leu Leu Val Asp Ala Val Ser 
65                  70                  75                  80  


Gln Asn Ala Ala Phe Leu Glu Gln Thr Leu Ser Ser Thr Ile Lys Gln 
                85                  90                  95      


Asp Asp Phe Thr Ala Arg Leu Phe Asp Ile His Lys Gln Val Leu Lys 
            100                 105                 110         


Glu Gly Ile Ala Gln Thr Val Phe Leu Gly Leu Asn Arg Ser Asp Tyr 
        115                 120                 125             


Met Phe Gln Arg Ser Ala Asp Gly Ser Pro Ala Leu Lys Gln Ile Glu 
    130                 135                 140                 


Ile Asn Thr Ile Ser Ala Ser Phe Gly Gly Leu Ala Ser Arg Thr Pro 
145                 150                 155                 160 


Ala Val His Arg His Val Leu Ser Val Leu Ser Lys Thr Lys Glu Ala 
                165                 170                 175     


Gly Lys Ile Leu Ser Asn Asn Pro Ser Lys Gly Leu Ala Leu Gly Ile 
            180                 185                 190         


Ala Lys Ala Trp Glu Leu Tyr Gly Ser Pro Asn Ala Leu Val Leu Leu 
        195                 200                 205             


Ile Ala Gln Glu Lys Glu Arg Asn Ile Phe Asp Gln Arg Ala Ile Glu 
    210                 215                 220                 


Asn Glu Leu Leu Ala Arg Asn Ile His Val Ile Arg Arg Thr Phe Glu 
225                 230                 235                 240 


Asp Ile Ser Glu Lys Gly Ser Leu Asp Gln Asp Arg Arg Leu Phe Val 
                245                 250                 255     


Asp Gly Gln Glu Ile Ala Val Val Tyr Phe Arg Asp Gly Tyr Met Pro 
            260                 265                 270         


Arg Gln Tyr Ser Leu Gln Asn Trp Glu Ala Arg Leu Leu Leu Glu Arg 
        275                 280                 285             


Ser His Ala Ala Lys Cys Pro Asp Ile Ala Thr Gln Leu Ala Gly Thr 
    290                 295                 300                 


Lys Lys Val Gln Gln Glu Leu Ser Arg Pro Gly Met Leu Glu Met Leu 
305                 310                 315                 320 


Leu Pro Gly Gln Pro Glu Ala Val Ala Arg Leu Arg Ala Thr Phe Ala 
                325                 330                 335     


Gly Leu Tyr Ser Leu Asp Val Gly Glu Glu Gly Asp Gln Ala Ile Ala 
            340                 345                 350         


Glu Ala Leu Ala Ala Pro Ser Arg Phe Val Leu Lys Pro Gln Arg Glu 
        355                 360                 365             


Gly Gly Gly Asn Asn Leu Tyr Gly Glu Glu Met Val Gln Ala Leu Lys 
    370                 375                 380                 


Gln Leu Lys Asp Ser Glu Glu Arg Ala Ser Tyr Ile Leu Met Glu Lys 
385                 390                 395                 400 


Ile Glu Pro Glu Pro Phe Glu Asn Cys Leu Leu Arg Pro Gly Ser Pro 
                405                 410                 415     


Ala Arg Val Val Gln Cys Ile Ser Glu Leu Gly Ile Phe Gly Val Tyr 
            420                 425                 430         


Val Arg Gln Glu Lys Thr Leu Val Met Asn Lys His Val Gly His Leu 
        435                 440                 445             


Leu Arg Thr Lys Ala Ile Glu His Ala Asp Gly Gly Val Ala Ala Gly 
    450                 455                 460                 


Val Ala Val Leu Asp Asn Pro Tyr Pro Val 
465                 470                 


