                         SEQUENCE LISTING

<110>  Estibio ApS
       Mikkelsen, Marie  J
       Kvist, Thomas
       Sondt-Marcussed, Line
 
<120>  Marker-free isolation of selected microorganisms with a known 
       genetic element

<130>  P1411PC00

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  26
<212>  DNA
<213>  Thermoanaerobacter sp.


<220>
<221>  primer_bind
<222>  (1)..(26)
<223>  ldhup1F forward primer

<400>  1
ttccatatct gtaagtcccg ctaaag                                            26


<210>  2
<211>  26
<212>  DNA
<213>  Thermoanaerobacter sp.


<220>
<221>  primer_bind
<222>  (1)..(26)
<223>  ldhup2 Reverse primer

<400>  2
attaatacaa tagttttgac aaatcc                                            26


<210>  3
<211>  35
<212>  DNA
<213>  Escherichia coli


<220>
<221>  primer_bind
<222>  (1)..(35)
<223>  parM-ext-Fw forward primer for parM-ext sequence in E. coli 
       plasmid R1/R100

<400>  3
ccccccgtta acatcaaact acagtggcag gaaag                                  35


<210>  4
<211>  41
<212>  DNA
<213>  Escherichia coli


<220>
<221>  primer_bind
<222>  (1)..(41)
<223>  par-M-ext-re  reverse primer for parM-ext sequence in E. coli 
       plasmid R1/R100

<400>  4
cccccctgca gcgttgcttc agatagttat tatcttttct g                           41


<210>  5
<211>  22
<212>  DNA
<213>  Thermoanaerobacter sp.


<220>
<221>  primer_bind
<222>  (1)..(22)
<223>  ldhdown3F forward primer

<400>  5
atataaaaag tcacagtgtg aa                                                22


<210>  6
<211>  22
<212>  DNA
<213>  Thermoanaerobacter sp.


<220>
<221>  primer_bind
<222>  (1)..(22)
<223>  ldhdown4R reverse primer

<400>  6
cacctatttt gcactttttt tc                                                22


<210>  7
<211>  21
<212>  DNA
<213>  Escherichia coli


<220>
<221>  primer_bind
<222>  (1)..(21)
<223>  par-Mext-Fw3  forward primer for parM-ext sequence in E. coli 
       plasmid R1/R100

<400>  7
ggcaatacag cgacgttaat g                                                 21


<210>  8
<211>  41
<212>  DNA
<213>  Escherichia coli


<220>
<221>  primer_bind
<222>  (1)..(41)
<223>  par-M-ext-Re  reverse primer for parM-ext sequence in E. coli 
       plasmid R1/R100

<400>  8
cccccctgca gcgttgcttc agatagttat tatcttttct g                           41


<210>  9
<211>  510
<212>  DNA
<213>  Escherichia coli


<220>
<221>  gene
<222>  (1)..(510)
<223>  PAR-M-ext fragment  from E. coli plasmid R1/R100

<400>  9
ggcaatacag cgacgttaat gtcgttgcag tgcatcacgc cttactgacc agtggtctgc       60

cggtaagcga agtggatatt gtttgcacac ttcctctgac agagtattac gacagaaata      120

accaacccaa tacggaaaat attgagcgta agaaagcaaa cttccggaaa aaaattacat      180

taaatggcgg ggatacattc acaataaaag atgtaaaagt catgcctgaa tctataccgg      240

caggttatga agttctacaa gaactggatg agttagattc tttattaatt atagatctcg      300

ggggcaccac attagatatt tctcaggtaa tggggaaatt atcggggatc agtaaaatgt      360

acggagactc atctcttggt gtctctctgg ttacatctgc agtaaaagat gccctttctc      420

ttgcgagaac aaaaggaagt agctatcttg ctgacgatat aatcattcac agaaaagata      480

ataactatct gaagcaacgc tgcagggggg                                       510


<210>  10
<211>  22
<212>  DNA
<213>  Thermoanaerobacter sp.


<220>
<221>  primer_bind
<222>  (1)..(22)
<223>  Primers LDH-out-Up  forward primer

<400>  10
gagctgcttt aagtgtctca gg                                                22


<210>  11
<211>  24
<212>  DNA
<213>  Thermoanaerobacter sp.


<220>
<221>  primer_bind
<222>  (1)..(24)
<223>  LDH-out-Dn3  reverse primer

<400>  11
gaagtggatc ctttataggc cggt                                              24


<210>  12
<211>  23
<212>  DNA
<213>  Pantoea ananatis


<220>
<221>  primer_bind
<222>  (1)..(23)
<223>  budC_det_247_forward  primer for BudC gene from Pantoea ananatis

<400>  12
aacgtsattg tgaataacgc mgg                                               23


<210>  13
<211>  20
<212>  DNA
<213>  Pantoea ananatis


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  budC_det_684_reverse  primer or BudC gene from Pantoea ananatis

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is a, c, g, or t

<400>  13
atcttccggc tcnganaggc                                                   20


<210>  14
<211>  341
<212>  DNA
<213>  Pantoea ananatis


<220>
<221>  gene
<222>  (1)..(341)
<223>  BudC gene encoding 2,3-butanediol dehydrogenase from Pantoea 
       ananatis

<400>  14
aacgtcattg tgaataacgc aggtgtcgcg ccctcaacgc ccattgatga aatcaccgaa       60

gaggtcatca acaaggttta tgacgttaat gtcaaaggcg tgatctgggg catgcaggcc      120

gcaataaaag cgttcgcgtc tgaaggtcat ggcggaaaaa ttattaacgc ctgttctcag      180

gccgggcatg tcggcaatcc cgagctggca gtttacagct caagcaagtt tgctgttcgg      240

ggcctgacac aaactgccgc acgggatctg gcaccggcgg ggatcaccgt gaatggcttt      300

tgtcctggta ttgttaaaac accgatgtgg gcggagatcg a                          341


<210>  15
<211>  25
<212>  DNA
<213>  Thermoanaerobacter


<220>
<221>  primer_bind
<222>  (1)..(25)
<223>  PTA1-out-up-F primer

<400>  15
ggtaaaggtg tccgtagtga aaagc                                             25


<210>  16
<211>  23
<212>  DNA
<213>  Thermoanaerobacter


<220>
<221>  primer_bind
<222>  (1)..(23)
<223>  AK1-out-dn1-re primer

<400>  16
ccaatactct caacgtcttc cac                                               23


<210>  17
<211>  21
<212>  DNA
<213>  Thermoanaerobacter


<220>
<221>  protein_bind
<222>  (1)..(21)
<223>  14K-fw2  primer

<400>  17
cagaaccgaa accacaaagc c                                                 21


<210>  18
<211>  21
<212>  DNA
<213>  Thermoanaerobacter


<220>
<221>  primer_bind
<222>  (1)..(21)
<223>  14K-re2  primer

<400>  18
ttacgagcgt gtactgagaa c                                                 21


