
                                SEQUENCE LISTING

<110> GlaxoSmithKline Biologicals SA

<120> Immunogenic Combination
  

<130> VB65359

<160> 56

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 463
<212> PRT
<213> Clostridium Difficile

<400> 1
Met Lys Lys Phe Arg Lys His Lys Arg Ile Ser Asn Cys Ile Ser Ile
 1               5                  10                  15      
Leu Leu Ile Leu Tyr Leu Thr Leu Gly Gly Leu Leu Pro Asn Asn Ile
            20                  25                  30          
Tyr Ala Gln Asp Leu Gln Ser Tyr Ser Glu Lys Val Cys Asn Thr Thr
        35                  40                  45              
Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp Phe Leu Lys Asp Lys Glu
    50                  55                  60                  
Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala Glu Arg Ile Glu Gln Lys
65                  70                  75                  80  
Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu Ser Tyr Lys Lys Asp Ser
                85                  90                  95      
Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg Asn Tyr Phe Tyr Asp Tyr
            100                 105                 110         
Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu Tyr Lys Glu Leu Arg Asn
        115                 120                 125             
Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro Met Tyr Val Tyr Tyr Phe
    130                 135                 140                 
Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys Val Ile Arg Thr Glu Asn
145                 150                 155                 160 
Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn Glu Phe Lys Glu Thr Ile
                165                 170                 175     
Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe Lys Asp Ile Ser Leu Tyr
            180                 185                 190         
Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr Pro Leu Leu Met His Leu
        195                 200                 205             
Lys Leu Pro Arg Asn Thr Gly Met Leu Pro Tyr Thr Asn Thr Asn Asn
    210                 215                 220                 
Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser Ile Lys Ile Asp Lys Ile
225                 230                 235                 240 
Val Arg Ile Val Ile Asp Gly Lys His Tyr Ile Lys Ala Glu Ala Ser
                245                 250                 255     
Val Val Ser Ser Leu Asp Phe Lys Asp Asp Val Ser Lys Gly Asp Ser
            260                 265                 270         
Trp Gly Lys Ala Asn Tyr Asn Asp Trp Ser Asn Lys Leu Thr Pro Asn
        275                 280                 285             
Glu Leu Ala Asp Val Asn Asp Tyr Met Arg Gly Gly Tyr Thr Ala Ile
    290                 295                 300                 
Asn Asn Tyr Leu Ile Ser Asn Gly Pro Val Asn Asn Pro Asn Pro Glu
305                 310                 315                 320 
Leu Asp Ser Lys Ile Thr Asn Ile Glu Asn Ala Leu Lys Arg Glu Pro
                325                 330                 335     
Ile Pro Thr Asn Leu Thr Val Tyr Arg Arg Ser Gly Pro Gln Glu Phe
            340                 345                 350         
Gly Leu Thr Leu Thr Ser Pro Glu Tyr Asp Phe Asn Lys Leu Glu Asn
        355                 360                 365             
Ile Asp Ala Phe Lys Ser Lys Trp Glu Gly Gln Ala Leu Ser Tyr Pro
    370                 375                 380                 
Asn Phe Ile Ser Thr Ser Ile Gly Ser Val Asn Met Ser Ala Phe Ala
385                 390                 395                 400 
Lys Arg Lys Ile Val Leu Arg Ile Thr Ile Pro Lys Gly Ser Pro Gly
                405                 410                 415     
Ala Tyr Leu Ser Ala Ile Pro Gly Tyr Ala Gly Glu Tyr Glu Val Leu
            420                 425                 430         
Leu Asn His Gly Ser Lys Phe Lys Ile Asn Lys Ile Asp Ser Tyr Lys
        435                 440                 445             
Asp Gly Thr Ile Thr Lys Leu Ile Val Asp Ala Thr Leu Ile Pro
    450                 455                 460             


<210> 2
<211> 1392
<212> DNA
<213> Clostridium Difficile

<400> 2
atgaaaaaat ttaggaaaca taaaaggatt agtaattgta tatctatatt gttgatatta 60
tatctaactt taggtggttt gttacctaat aacatttatg cacaagactt acaaagctat 120
agtgaaaaag tttgcaatac tacttacaag gctcctatag aaagaccaga agattttctt 180
aaagataaag aaaaggctaa agaatgggaa agaaaagaag cagaaagaat agagcaaaaa 240
cttgaaagat ctgaaaaaga agcattagaa tcatataaaa aagattctgt agaaataagt 300
aaatattctc agacaagaaa ttatttttat gattatcaaa tagaagcaaa ttctcgagaa 360
aaagaatata aagaacttcg aaatgctata tcaaaaaata aaatagataa acctatgtat 420
gtctattatt ttgaatctcc agaaaaattt gcatttaata aagtaataag aacagaaaat 480
caaaacgaaa tttcattaga aaaatttaat gagtttaaag aaactataca aaacaaatta 540
tttaagcaag atggatttaa agatatttct ttatatgaac ctggaaaagg tgatgaaaaa 600
cctacaccat tacttatgca cttaaaatta cctagaaata ctggtatgtt accatataca 660
aatactaaca atgtaagtac attaatagag caaggatata gtataaaaat agataaaatt 720
gttcgtatag ttatagatgg gaagcactat attaaagcag aagcatctgt tgtaagtagt 780
cttgatttta aagatgatgt aagtaagggg gattcttggg gtaaagcaaa ttataatgat 840
tggagtaata aattaacacc taatgaactt gctgatgtaa atgattatat gcgtggagga 900
tatactgcaa ttaataatta tttaatatca aatggtccag taaataatcc taacccagaa 960
ttagattcta aaatcacaaa cattgaaaat gcattaaaac gtgaacctat tccaactaat 1020
ttaactgtat atagaagatc tggtcctcaa gaatttggtt taactcttac ttcccctgaa 1080
tatgatttta acaaactaga aaatatagat gcttttaaat caaaatggga aggacaagca 1140
ctgtcttatc caaactttat tagtactagt attggtagtg tgaatatgag tgcatttgct 1200
aaaagaaaaa tagtactacg tataactata cctaaaggtt ctcctggagc ttatctatca 1260
gctattccag gttatgcagg tgaatatgaa gtgcttttaa atcatggaag caaatttaaa 1320
atcaataaaa ttgattctta caaagatggt actataacaa aattaattgt tgatgcaaca 1380
ttgatacctt aa                                                     1392

<210> 3
<211> 876
<212> PRT
<213> Clostridium Difficile

<400> 3
Met Lys Ile Gln Met Arg Asn Lys Lys Val Leu Ser Phe Leu Thr Leu
 1               5                  10                  15      
Thr Ala Ile Val Ser Gln Ala Leu Val Tyr Pro Val Tyr Ala Gln Thr
            20                  25                  30          
Ser Thr Ser Asn His Ser Asn Lys Lys Lys Glu Ile Val Asn Glu Asp
        35                  40                  45              
Ile Leu Pro Asn Asn Gly Leu Met Gly Tyr Tyr Phe Thr Asp Glu His
    50                  55                  60                  
Phe Lys Asp Leu Lys Leu Met Ala Pro Ile Lys Asp Gly Asn Leu Lys
65                  70                  75                  80  
Phe Glu Glu Lys Lys Val Asp Lys Leu Leu Asp Lys Asp Lys Ser Asp
                85                  90                  95      
Val Lys Ser Ile Arg Trp Thr Gly Arg Ile Ile Pro Ser Lys Asp Gly
            100                 105                 110         
Glu Tyr Thr Leu Ser Thr Asp Arg Asp Asp Val Leu Met Gln Val Asn
        115                 120                 125             
Thr Glu Ser Thr Ile Ser Asn Thr Leu Lys Val Asn Met Lys Lys Gly
    130                 135                 140                 
Lys Glu Tyr Lys Val Arg Ile Glu Leu Gln Asp Lys Asn Leu Gly Ser
145                 150                 155                 160 
Ile Asp Asn Leu Ser Ser Pro Asn Leu Tyr Trp Glu Leu Asp Gly Met
                165                 170                 175     
Lys Lys Ile Ile Pro Glu Glu Asn Leu Phe Leu Arg Asp Tyr Ser Asn
            180                 185                 190         
Ile Glu Lys Asp Asp Pro Phe Ile Pro Asn Asn Asn Phe Phe Asp Pro
        195                 200                 205             
Lys Leu Met Ser Asp Trp Glu Asp Glu Asp Leu Asp Thr Asp Asn Asp
    210                 215                 220                 
Asn Ile Pro Asp Ser Tyr Glu Arg Asn Gly Tyr Thr Ile Lys Asp Leu
225                 230                 235                 240 
Ile Ala Val Lys Trp Glu Asp Ser Phe Ala Glu Gln Gly Tyr Lys Lys
                245                 250                 255     
Tyr Val Ser Asn Tyr Leu Glu Ser Asn Thr Ala Gly Asp Pro Tyr Thr
            260                 265                 270         
Asp Tyr Glu Lys Ala Ser Gly Ser Phe Asp Lys Ala Ile Lys Thr Glu
        275                 280                 285             
Ala Arg Asp Pro Leu Val Ala Ala Tyr Pro Ile Val Gly Val Gly Met
    290                 295                 300                 
Glu Lys Leu Ile Ile Ser Thr Asn Glu His Ala Ser Thr Asp Gln Gly
305                 310                 315                 320 
Lys Thr Val Ser Arg Ala Thr Thr Asn Ser Lys Thr Glu Ser Asn Thr
                325                 330                 335     
Ala Gly Val Ser Val Asn Val Gly Tyr Gln Asn Gly Phe Thr Ala Asn
            340                 345                 350         
Val Thr Thr Asn Tyr Ser His Thr Thr Asp Asn Ser Thr Ala Val Gln
        355                 360                 365             
Asp Ser Asn Gly Glu Ser Trp Asn Thr Gly Leu Ser Ile Asn Lys Gly
    370                 375                 380                 
Glu Ser Ala Tyr Ile Asn Ala Asn Val Arg Tyr Tyr Asn Thr Gly Thr
385                 390                 395                 400 
Ala Pro Met Tyr Lys Val Thr Pro Thr Thr Asn Leu Val Leu Asp Gly
                405                 410                 415     
Asp Thr Leu Ser Thr Ile Lys Ala Gln Glu Asn Gln Ile Gly Asn Asn
            420                 425                 430         
Leu Ser Pro Gly Asp Thr Tyr Pro Lys Lys Gly Leu Ser Pro Leu Ala
        435                 440                 445             
Leu Asn Thr Met Asp Gln Phe Ser Ser Arg Leu Ile Pro Ile Asn Tyr
    450                 455                 460                 
Asp Gln Leu Lys Lys Leu Asp Ala Gly Lys Gln Ile Lys Leu Glu Thr
465                 470                 475                 480 
Thr Gln Val Ser Gly Asn Phe Gly Thr Lys Asn Ser Ser Gly Gln Ile
                485                 490                 495     
Val Thr Glu Gly Asn Ser Trp Ser Asp Tyr Ile Ser Gln Ile Asp Ser
            500                 505                 510         
Ile Ser Ala Ser Ile Ile Leu Asp Thr Glu Asn Glu Ser Tyr Glu Arg
        515                 520                 525             
Arg Val Thr Ala Lys Asn Leu Gln Asp Pro Glu Asp Lys Thr Pro Glu
    530                 535                 540                 
Leu Thr Ile Gly Glu Ala Ile Glu Lys Ala Phe Gly Ala Thr Lys Lys
545                 550                 555                 560 
Asp Gly Leu Leu Tyr Phe Asn Asp Ile Pro Ile Asp Glu Ser Cys Val
                565                 570                 575     
Glu Leu Ile Phe Asp Asp Asn Thr Ala Asn Lys Ile Lys Asp Ser Leu
            580                 585                 590         
Lys Thr Leu Ser Asp Lys Lys Ile Tyr Asn Val Lys Leu Glu Arg Gly
        595                 600                 605             
Met Asn Ile Leu Ile Lys Thr Pro Thr Tyr Phe Thr Asn Phe Asp Asp
    610                 615                 620                 
Tyr Asn Asn Tyr Pro Ser Thr Trp Ser Asn Val Asn Thr Thr Asn Gln
625                 630                 635                 640 
Asp Gly Leu Gln Gly Ser Ala Asn Lys Leu Asn Gly Glu Thr Lys Ile
                645                 650                 655     
Lys Ile Pro Met Ser Glu Leu Lys Pro Tyr Lys Arg Tyr Val Phe Ser
            660                 665                 670         
Gly Tyr Ser Lys Asp Pro Leu Thr Ser Asn Ser Ile Ile Val Lys Ile
        675                 680                 685             
Lys Ala Lys Glu Glu Lys Thr Asp Tyr Leu Val Pro Glu Gln Gly Tyr
    690                 695                 700                 
Thr Lys Phe Ser Tyr Glu Phe Glu Thr Thr Glu Lys Asp Ser Ser Asn
705                 710                 715                 720 
Ile Glu Ile Thr Leu Ile Gly Ser Gly Thr Thr Tyr Leu Asp Asn Leu
                725                 730                 735     
Ser Ile Thr Glu Leu Asn Ser Thr Pro Glu Ile Leu Asp Glu Pro Glu
            740                 745                 750         
Val Lys Ile Pro Thr Asp Gln Glu Ile Met Asp Ala His Lys Ile Tyr
        755                 760                 765             
Phe Ala Asp Leu Asn Phe Asn Pro Ser Thr Gly Asn Thr Tyr Ile Asn
    770                 775                 780                 
Gly Met Tyr Phe Ala Pro Thr Gln Thr Asn Lys Glu Ala Leu Asp Tyr
785                 790                 795                 800 
Ile Gln Lys Tyr Arg Val Glu Ala Thr Leu Gln Tyr Ser Gly Phe Lys
                805                 810                 815     
Asp Ile Gly Thr Lys Asp Lys Glu Met Arg Asn Tyr Leu Gly Asp Pro
            820                 825                 830         
Asn Gln Pro Lys Thr Asn Tyr Val Asn Leu Arg Ser Tyr Phe Thr Gly
        835                 840                 845             
Gly Glu Asn Ile Met Thr Tyr Lys Lys Leu Arg Ile Tyr Ala Ile Thr
    850                 855                 860                 
Pro Asp Asp Arg Glu Leu Leu Val Leu Ser Val Asp
865                 870                 875     


<210> 4
<211> 2631
<212> DNA
<213> Clostridium Difficile

<400> 4
atgaaaatac aaatgaggaa taaaaaggta ttaagttttt taacacttac agctatagtt 60
agtcaagcac tagtatatcc tgtatatgct caaactagta caagtaatca ttctaataag 120
aaaaaagaaa ttgtaaatga agatatactc ccaaacaatg gattaatggg atattatttc 180
acagatgagc actttaaaga tttaaaatta atggcaccca taaaagatgg taatttaaaa 240
tttgaagaaa agaaagtaga taaacttctg gataaagaca aatcagatgt aaaatctata 300
cgatggacag gaagaataat tccttctaag gatggtgaat atacattatc aactgataga 360
gatgatgtct taatgcaagt aaatactgag agtactatat caaatacact taaagttaat 420
atgaaaaagg gtaaagaata taaagttaga atagagctac aagataaaaa tttaggttca 480
atagataatt tatcatcacc taatctttat tgggaattag atggtatgaa gaaaattata 540
ccagaagaaa atttattctt aagagattat tctaatatag aaaaagatga tccatttatc 600
ccaaataaca atttctttga cccaaagttg atgtctgatt gggaagacga agatttggat 660
acagataatg ataatatacc agattcatat gaacgaaatg gatatactat taaggactta 720
attgcagtta agtgggaaga tagttttgca gaacaaggct ataagaaata tgtatcaaat 780
tatttagagt caaatactgc tggagatcca tatacagatt atgaaaaagc ttcaggttct 840
tttgacaagg ctataaagac tgaagcaaga gatccgttag ttgcagcata tccaattgtt 900
ggagtaggta tggaaaaatt aattatatct acaaatgaac atgcctctac tgatcaaggt 960
aaaactgttt ccagagctac tactaacagt aaaactgaat ctaatacagc tggtgtgtct 1020
gttaatgtag gatatcaaaa tggattcaca gctaatgtaa ctacaaatta ttcccataca 1080
acagataatt caactgctgt tcaagatagt aatggagaat catggaatac tggattaagt 1140
ataaacaaag gagaatctgc atatataaat gcaaatgtta gatattacaa cacaggtact 1200
gcacctatgt acaaagtgac accaacaaca aatttagtgt tagatggaga tacattatca 1260
actatcaaag cacaagaaaa tcaaattggc aataatctat ctcctggaga tacttatccc 1320
aaaaaagggc tttcacctct agctcttaac acaatggatc aatttagctc tagactgatt 1380
cctataaatt atgatcaatt aaaaaaatta gatgctggaa agcaaattaa attagaaaca 1440
acacaagtaa gtggaaattt tggtacaaaa aatagttctg gacaaatagt aacagaagga 1500
aatagttggt cagactatat aagtcaaatt gacagtattt ctgcatctat tatattagat 1560
acagagaatg aatcttacga aagaagagtt actgctaaaa atttacagga tccagaagat 1620
aaaacacctg aacttacaat tggagaagca attgaaaaag cttttggcgc tactaaaaaa 1680
gatggtttgt tatattttaa tgatatacca atagatgaaa gttgtgttga actcatattt 1740
gatgataata cagccaataa gattaaagat agtttaaaaa ctttgtctga taaaaagata 1800
tataatgtta aacttgaaag aggaatgaat atacttataa aaacaccaac ttactttact 1860
aattttgatg attataataa ttaccctagt acatggagta atgtcaatac tacgaatcaa 1920
gatggtttac aaggctcagc aaataaatta aatggtgaga cgaagattaa aatccctatg 1980
tctgagctaa aaccttataa acgttatgtt tttagtggat attcaaagga tcctttaaca 2040
tctaattcaa taattgtaaa gataaaagca aaagaagaga aaacggatta tttggtacca 2100
gaacaaggat atacaaaatt tagttatgaa tttgaaacta ctgaaaaaga ttcttctaat 2160
atagagataa cattaattgg tagtggtaca acatacttag ataacttatc tattacagag 2220
ctaaatagta ctcctgaaat acttgatgaa ccagaagtta aaattccaac tgaccaagaa 2280
ataatggatg cacataaaat atattttgca gatttaaatt ttaatccaag tacaggaaat 2340
acttatataa atggtatgta ttttgcacca acacaaacta ataaagaagc tctcgattat 2400
atccaaaaat atagagttga agctacttta caatattctg gatttaaaga tattggaact 2460
aaagataaag aaatgcgtaa ttatttagga gatccaaatc agcctaaaac taattatgtt 2520
aatcttagga gttattttac aggtggagaa aatattatga catacaagaa attaagaata 2580
tatgcaatta ctccagacga tagagagtta ttagttctta gtgttgatta g          2631

<210> 5
<211> 421
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDTa C34 construct polypeptide

<400> 5
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Ser Leu Asp Phe Lys Asp Asp
    210                 215                 220                 
Val Ser Lys Gly Asp Ser Trp Gly Lys Ala Asn Tyr Asn Asp Trp Ser
225                 230                 235                 240 
Asn Lys Leu Thr Pro Asn Glu Leu Ala Asp Val Asn Asp Tyr Met Arg
                245                 250                 255     
Gly Gly Tyr Thr Ala Ile Asn Asn Tyr Leu Ile Ser Asn Gly Pro Val
            260                 265                 270         
Asn Asn Pro Asn Pro Glu Leu Asp Ser Lys Ile Thr Asn Ile Glu Asn
        275                 280                 285             
Ala Leu Lys Arg Glu Pro Ile Pro Thr Asn Leu Thr Val Tyr Arg Arg
    290                 295                 300                 
Ser Gly Pro Gln Glu Phe Gly Leu Thr Leu Thr Ser Pro Glu Tyr Asp
305                 310                 315                 320 
Phe Asn Lys Leu Glu Asn Ile Asp Ala Phe Lys Ser Lys Trp Glu Gly
                325                 330                 335     
Gln Ala Leu Ser Tyr Pro Asn Phe Ile Ser Thr Ser Ile Gly Ser Val
            340                 345                 350         
Asn Met Ser Ala Phe Ala Lys Arg Lys Ile Val Leu Arg Ile Thr Ile
        355                 360                 365             
Pro Lys Gly Ser Pro Gly Ala Tyr Leu Ser Ala Ile Pro Gly Tyr Ala
    370                 375                 380                 
Gly Glu Tyr Glu Val Leu Leu Asn His Gly Ser Lys Phe Lys Ile Asn
385                 390                 395                 400 
Lys Ile Asp Ser Tyr Lys Asp Gly Thr Ile Thr Lys Leu Ile Val Asp
                405                 410                 415     
Ala Thr Leu Ile Pro
            420     


<210> 6
<211> 1266
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDTb C34 construct polynucleotide

<400> 6
Ala Thr Gly Gly Thr Thr Thr Gly Cys Ala Ala Thr Ala Cys Cys Ala
 1               5                  10                  15      
Cys Cys Thr Ala Thr Ala Ala Ala Gly Cys Ala Cys Cys Gly Ala Thr
            20                  25                  30          
Thr Gly Ala Ala Cys Gly Thr Cys Cys Gly Gly Ala Ala Gly Ala Thr
        35                  40                  45              
Thr Thr Thr Cys Thr Gly Ala Ala Ala Gly Ala Thr Ala Ala Ala Gly
    50                  55                  60                  
Ala Ala Ala Ala Ala Gly Cys Cys Ala Ala Ala Gly Ala Ala Thr Gly
65                  70                  75                  80  
Gly Gly Ala Ala Cys Gly Cys Ala Ala Ala Gly Ala Ala Gly Cys Ala
                85                  90                  95      
Gly Ala Ala Cys Gly Thr Ala Thr Thr Gly Ala Ala Cys Ala Gly Ala
            100                 105                 110         
Ala Ala Cys Thr Gly Gly Ala Ala Cys Gly Thr Ala Gly Cys Gly Ala
        115                 120                 125             
Ala Ala Ala Ala Gly Ala Ala Gly Cys Ala Cys Thr Gly Gly Ala Ala
    130                 135                 140                 
Ala Gly Cys Thr Ala Cys Ala Ala Ala Ala Ala Ala Gly Ala Thr Ala
145                 150                 155                 160 
Gly Cys Gly Thr Gly Gly Ala Ala Ala Thr Thr Thr Cys Ala Ala Ala
                165                 170                 175     
Ala Thr Ala Thr Ala Gly Cys Cys Ala Gly Ala Cys Cys Cys Gly Cys
            180                 185                 190         
Ala Ala Thr Thr Ala Thr Thr Thr Cys Thr Ala Thr Gly Ala Thr Thr
        195                 200                 205             
Ala Thr Cys Ala Gly Ala Thr Thr Gly Ala Ala Gly Cys Cys Ala Ala
    210                 215                 220                 
Thr Ala Gly Cys Cys Gly Thr Gly Ala Ala Ala Ala Ala Gly Ala Ala
225                 230                 235                 240 
Thr Ala Thr Ala Ala Ala Gly Ala Ala Cys Thr Gly Cys Gly Cys Ala
                245                 250                 255     
Ala Thr Gly Cys Cys Ala Thr Thr Ala Gly Cys Ala Ala Ala Ala Ala
            260                 265                 270         
Cys Ala Ala Ala Ala Thr Thr Gly Ala Thr Ala Ala Ala Cys Cys Gly
        275                 280                 285             
Ala Thr Gly Thr Ala Thr Gly Thr Gly Thr Ala Thr Thr Ala Thr Thr
    290                 295                 300                 
Thr Cys Gly Ala Ala Ala Gly Thr Cys Cys Gly Gly Ala Ala Ala Ala
305                 310                 315                 320 
Ala Thr Thr Thr Gly Cys Cys Thr Thr Thr Ala Ala Cys Ala Ala Ala
                325                 330                 335     
Gly Thr Gly Ala Thr Thr Cys Gly Cys Ala Cys Cys Gly Ala Ala Ala
            340                 345                 350         
Ala Thr Cys Ala Gly Ala Ala Thr Gly Ala Ala Ala Thr Thr Ala Gly
        355                 360                 365             
Cys Cys Thr Gly Gly Ala Ala Ala Ala Ala Thr Thr Cys Ala Ala Thr
    370                 375                 380                 
Gly Ala Ala Thr Thr Thr Ala Ala Ala Gly Ala Ala Ala Cys Cys Ala
385                 390                 395                 400 
Thr Thr Cys Ala Gly Ala Ala Thr Ala Ala Ala Cys Thr Gly Thr Thr
                405                 410                 415     
Thr Ala Ala Ala Cys Ala Gly Gly Ala Thr Gly Gly Cys Thr Thr Thr
            420                 425                 430         
Ala Ala Ala Gly Ala Thr Ala Thr Thr Thr Cys Ala Cys Thr Gly Thr
        435                 440                 445             
Ala Thr Gly Ala Ala Cys Cys Gly Gly Gly Thr Ala Ala Ala Gly Gly
    450                 455                 460                 
Thr Gly Ala Thr Gly Ala Ala Ala Ala Ala Cys Cys Gly Ala Cys Ala
465                 470                 475                 480 
Cys Cys Gly Cys Thr Gly Cys Thr Gly Ala Thr Gly Cys Ala Thr Cys
                485                 490                 495     
Thr Gly Ala Ala Ala Cys Thr Gly Cys Cys Thr Cys Gly Thr Ala Ala
            500                 505                 510         
Thr Ala Cys Cys Gly Gly Thr Ala Thr Gly Cys Thr Gly Cys Cys Gly
        515                 520                 525             
Thr Ala Thr Ala Cys Cys Ala Ala Thr Ala Cys Cys Ala Ala Thr Ala
    530                 535                 540                 
Ala Thr Gly Thr Thr Ala Gly Cys Ala Cys Cys Cys Thr Gly Ala Thr
545                 550                 555                 560 
Thr Gly Ala Ala Cys Ala Gly Gly Gly Cys Thr Ala Thr Ala Gly Cys
                565                 570                 575     
Ala Thr Cys Ala Ala Ala Ala Thr Thr Gly Ala Thr Ala Ala Ala Ala
            580                 585                 590         
Thr Thr Gly Thr Gly Cys Gly Cys Ala Thr Thr Gly Thr Gly Ala Thr
        595                 600                 605             
Thr Gly Ala Thr Gly Gly Cys Ala Ala Ala Cys Ala Thr Thr Ala Thr
    610                 615                 620                 
Ala Thr Cys Ala Ala Ala Gly Cys Cys Gly Ala Ala Gly Cys Cys Ala
625                 630                 635                 640 
Gly Cys Gly Thr Thr Gly Thr Thr Thr Cys Ala Ala Gly Cys Cys Thr
                645                 650                 655     
Gly Gly Ala Thr Thr Thr Thr Ala Ala Ala Gly Ala Thr Gly Ala Thr
            660                 665                 670         
Gly Thr Gly Ala Gly Cys Ala Ala Ala Gly Gly Cys Gly Ala Thr Ala
        675                 680                 685             
Gly Cys Thr Gly Gly Gly Gly Thr Ala Ala Ala Gly Cys Ala Ala Ala
    690                 695                 700                 
Cys Thr Ala Thr Ala Ala Thr Gly Ala Thr Thr Gly Gly Ala Gly Cys
705                 710                 715                 720 
Ala Ala Thr Ala Ala Ala Cys Thr Gly Ala Cys Cys Cys Cys Gly Ala
                725                 730                 735     
Ala Thr Gly Ala Ala Cys Thr Gly Gly Cys Ala Gly Ala Thr Gly Thr
            740                 745                 750         
Gly Ala Ala Thr Gly Ala Thr Thr Ala Thr Ala Thr Gly Cys Gly Thr
        755                 760                 765             
Gly Gly Thr Gly Gly Thr Thr Ala Thr Ala Cys Cys Gly Cys Cys Ala
    770                 775                 780                 
Thr Thr Ala Ala Cys Ala Ala Thr Thr Ala Thr Cys Thr Gly Ala Thr
785                 790                 795                 800 
Thr Ala Gly Cys Ala Ala Thr Gly Gly Thr Cys Cys Gly Gly Thr Gly
                805                 810                 815     
Ala Ala Thr Ala Ala Thr Cys Cys Gly Ala Ala Thr Cys Cys Gly Gly
            820                 825                 830         
Ala Ala Cys Thr Gly Gly Ala Thr Ala Gly Cys Ala Ala Ala Ala Thr
        835                 840                 845             
Thr Ala Cys Cys Ala Ala Thr Ala Thr Thr Gly Ala Ala Ala Ala Thr
    850                 855                 860                 
Gly Cys Cys Cys Thr Gly Ala Ala Ala Cys Gly Cys Gly Ala Ala Cys
865                 870                 875                 880 
Cys Gly Ala Thr Thr Cys Cys Gly Ala Cys Cys Ala Ala Thr Cys Thr
                885                 890                 895     
Gly Ala Cys Cys Gly Thr Thr Thr Ala Thr Cys Gly Thr Cys Gly Thr
            900                 905                 910         
Ala Gly Cys Gly Gly Thr Cys Cys Gly Cys Ala Ala Gly Ala Ala Thr
        915                 920                 925             
Thr Thr Gly Gly Thr Cys Thr Gly Ala Cys Cys Cys Thr Gly Ala Cys
    930                 935                 940                 
Cys Ala Gly Thr Cys Cys Gly Gly Ala Ala Thr Ala Thr Gly Ala Cys
945                 950                 955                 960 
Thr Thr Thr Ala Ala Cys Ala Ala Ala Cys Thr Gly Gly Ala Ala Ala
                965                 970                 975     
Ala Thr Ala Thr Thr Gly Ala Thr Gly Cys Cys Thr Thr Thr Ala Ala
            980                 985                 990         
Ala Ala Gly Cys Ala Ala Ala Thr Gly Gly Gly Ala Ala Gly Gly Thr
        995                 1000                1005            
Cys Ala Gly Gly Cys Ala Cys Thr Gly Ala Gly Cys Thr Ala Thr Cys
    1010                1015                1020                
Cys Gly Ala Ala Cys Thr Thr Thr Ala Thr Thr Ala Gly Cys Ala Cys
1025                1030                1035                1040
Cys Ala Gly Cys Ala Thr Thr Gly Gly Thr Ala Gly Cys Gly Thr Thr
                1045                1050                1055    
Ala Ala Thr Ala Thr Gly Ala Gly Cys Gly Cys Ala Thr Thr Thr Gly
            1060                1065                1070        
Cys Cys Ala Ala Ala Cys Gly Thr Ala Ala Ala Ala Thr Thr Gly Thr
        1075                1080                1085            
Gly Cys Thr Gly Cys Gly Thr Ala Thr Thr Ala Cys Cys Ala Thr Thr
    1090                1095                1100                
Cys Cys Gly Ala Ala Ala Gly Gly Thr Ala Gly Thr Cys Cys Gly Gly
1105                1110                1115                1120
Gly Thr Gly Cys Ala Thr Ala Thr Cys Thr Gly Ala Gly Cys Gly Cys
                1125                1130                1135    
Ala Ala Thr Thr Cys Cys Gly Gly Gly Thr Thr Ala Thr Gly Cys Cys
            1140                1145                1150        
Gly Gly Thr Gly Ala Ala Thr Ala Thr Gly Ala Ala Gly Thr Thr Cys
        1155                1160                1165            
Thr Gly Cys Thr Gly Ala Ala Thr Cys Ala Thr Gly Gly Cys Ala Gly
    1170                1175                1180                
Cys Ala Ala Ala Thr Thr Cys Ala Ala Ala Ala Thr Thr Ala Ala Cys
1185                1190                1195                1200
Ala Ala Ala Ala Thr Thr Gly Ala Thr Ala Gly Cys Thr Ala Thr Ala
                1205                1210                1215    
Ala Ala Gly Ala Thr Gly Gly Cys Ala Cys Cys Ala Thr Thr Ala Cys
            1220                1225                1230        
Cys Ala Ala Ala Cys Thr Gly Ala Thr Thr Gly Thr Thr Gly Ala Thr
        1235                1240                1245            
Gly Cys Ala Ala Cys Cys Cys Thr Gly Ala Thr Thr Cys Cys Gly Thr
    1250                1255                1260                
Ala Ala
1265    


<210> 7
<211> 1067
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDTb C37 construct. CDTb' (minus signal peptide)
      ligated to Glutathione-S-transferase protein
      polypeptide

<400> 7
Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro
 1               5                  10                  15      
Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu
            20                  25                  30          
Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu
        35                  40                  45              
Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys
    50                  55                  60                  
Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn
65                  70                  75                  80  
Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu
                85                  90                  95      
Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser
            100                 105                 110         
Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu
        115                 120                 125             
Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn
    130                 135                 140                 
Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp
145                 150                 155                 160 
Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu
                165                 170                 175     
Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr
            180                 185                 190         
Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala
        195                 200                 205             
Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Leu Glu Val Leu
    210                 215                 220                 
Phe Gln Gly Pro Leu Gly Ser His Met Glu Ile Val Asn Glu Asp Ile
225                 230                 235                 240 
Leu Pro Asn Asn Gly Leu Met Gly Tyr Tyr Phe Thr Asp Glu His Phe
                245                 250                 255     
Lys Asp Leu Lys Leu Met Ala Pro Ile Lys Asp Gly Asn Leu Lys Phe
            260                 265                 270         
Glu Glu Lys Lys Val Asp Lys Leu Leu Asp Lys Asp Lys Ser Asp Val
        275                 280                 285             
Lys Ser Ile Arg Trp Thr Gly Arg Ile Ile Pro Ser Lys Asp Gly Glu
    290                 295                 300                 
Tyr Thr Leu Ser Thr Asp Arg Asp Asp Val Leu Met Gln Val Asn Thr
305                 310                 315                 320 
Glu Ser Thr Ile Ser Asn Thr Leu Lys Val Asn Met Lys Lys Gly Lys
                325                 330                 335     
Glu Tyr Lys Val Arg Ile Glu Leu Gln Asp Lys Asn Leu Gly Ser Ile
            340                 345                 350         
Asp Asn Leu Ser Ser Pro Asn Leu Tyr Trp Glu Leu Asp Gly Met Lys
        355                 360                 365             
Lys Ile Ile Pro Glu Glu Asn Leu Phe Leu Arg Asp Tyr Ser Asn Ile
    370                 375                 380                 
Glu Lys Asp Asp Pro Phe Ile Pro Asn Asn Asn Phe Phe Asp Pro Lys
385                 390                 395                 400 
Leu Met Ser Asp Trp Glu Asp Glu Asp Leu Asp Thr Asp Asn Asp Asn
                405                 410                 415     
Ile Pro Asp Ser Tyr Glu Arg Asn Gly Tyr Thr Ile Lys Asp Leu Ile
            420                 425                 430         
Ala Val Lys Trp Glu Asp Ser Phe Ala Glu Gln Gly Tyr Lys Lys Tyr
        435                 440                 445             
Val Ser Asn Tyr Leu Glu Ser Asn Thr Ala Gly Asp Pro Tyr Thr Asp
    450                 455                 460                 
Tyr Glu Lys Ala Ser Gly Ser Phe Asp Lys Ala Ile Lys Thr Glu Ala
465                 470                 475                 480 
Arg Asp Pro Leu Val Ala Ala Tyr Pro Ile Val Gly Val Gly Met Glu
                485                 490                 495     
Lys Leu Ile Ile Ser Thr Asn Glu His Ala Ser Thr Asp Gln Gly Lys
            500                 505                 510         
Thr Val Ser Arg Ala Thr Thr Asn Ser Lys Thr Glu Ser Asn Thr Ala
        515                 520                 525             
Gly Val Ser Val Asn Val Gly Tyr Gln Asn Gly Phe Thr Ala Asn Val
    530                 535                 540                 
Thr Thr Asn Tyr Ser His Thr Thr Asp Asn Ser Thr Ala Val Gln Asp
545                 550                 555                 560 
Ser Asn Gly Glu Ser Trp Asn Thr Gly Leu Ser Ile Asn Lys Gly Glu
                565                 570                 575     
Ser Ala Tyr Ile Asn Ala Asn Val Arg Tyr Tyr Asn Thr Gly Thr Ala
            580                 585                 590         
Pro Met Tyr Lys Val Thr Pro Thr Thr Asn Leu Val Leu Asp Gly Asp
        595                 600                 605             
Thr Leu Ser Thr Ile Lys Ala Gln Glu Asn Gln Ile Gly Asn Asn Leu
    610                 615                 620                 
Ser Pro Gly Asp Thr Tyr Pro Lys Lys Gly Leu Ser Pro Leu Ala Leu
625                 630                 635                 640 
Asn Thr Met Asp Gln Phe Ser Ser Arg Leu Ile Pro Ile Asn Tyr Asp
                645                 650                 655     
Gln Leu Lys Lys Leu Asp Ala Gly Lys Gln Ile Lys Leu Glu Thr Thr
            660                 665                 670         
Gln Val Ser Gly Asn Phe Gly Thr Lys Asn Ser Ser Gly Gln Ile Val
        675                 680                 685             
Thr Glu Gly Asn Ser Trp Ser Asp Tyr Ile Ser Gln Ile Asp Ser Ile
    690                 695                 700                 
Ser Ala Ser Ile Ile Leu Asp Thr Glu Asn Glu Ser Tyr Glu Arg Arg
705                 710                 715                 720 
Val Thr Ala Lys Asn Leu Gln Asp Pro Glu Asp Lys Thr Pro Glu Leu
                725                 730                 735     
Thr Ile Gly Glu Ala Ile Glu Lys Ala Phe Gly Ala Thr Lys Lys Asp
            740                 745                 750         
Gly Leu Leu Tyr Phe Asn Asp Ile Pro Ile Asp Glu Ser Cys Val Glu
        755                 760                 765             
Leu Ile Phe Asp Asp Asn Thr Ala Asn Lys Ile Lys Asp Ser Leu Lys
    770                 775                 780                 
Thr Leu Ser Asp Lys Lys Ile Tyr Asn Val Lys Leu Glu Arg Gly Met
785                 790                 795                 800 
Asn Ile Leu Ile Lys Thr Pro Thr Tyr Phe Thr Asn Phe Asp Asp Tyr
                805                 810                 815     
Asn Asn Tyr Pro Ser Thr Trp Ser Asn Val Asn Thr Thr Asn Gln Asp
            820                 825                 830         
Gly Leu Gln Gly Ser Ala Asn Lys Leu Asn Gly Glu Thr Lys Ile Lys
        835                 840                 845             
Ile Pro Met Ser Glu Leu Lys Pro Tyr Lys Arg Tyr Val Phe Ser Gly
    850                 855                 860                 
Tyr Ser Lys Asp Pro Leu Thr Ser Asn Ser Ile Ile Val Lys Ile Lys
865                 870                 875                 880 
Ala Lys Glu Glu Lys Thr Asp Tyr Leu Val Pro Glu Gln Gly Tyr Thr
                885                 890                 895     
Lys Phe Ser Tyr Glu Phe Glu Thr Thr Glu Lys Asp Ser Ser Asn Ile
            900                 905                 910         
Glu Ile Thr Leu Ile Gly Ser Gly Thr Thr Tyr Leu Asp Asn Leu Ser
        915                 920                 925             
Ile Thr Glu Leu Asn Ser Thr Pro Glu Ile Leu Asp Glu Pro Glu Val
    930                 935                 940                 
Lys Ile Pro Thr Asp Gln Glu Ile Met Asp Ala His Lys Ile Tyr Phe
945                 950                 955                 960 
Ala Asp Leu Asn Phe Asn Pro Ser Thr Gly Asn Thr Tyr Ile Asn Gly
                965                 970                 975     
Met Tyr Phe Ala Pro Thr Gln Thr Asn Lys Glu Ala Leu Asp Tyr Ile
            980                 985                 990         
Gln Lys Tyr Arg Val Glu Ala Thr Leu Gln Tyr Ser Gly Phe Lys Asp
        995                 1000                1005            
Ile Gly Thr Lys Asp Lys Glu Met Arg Asn Tyr Leu Gly Asp Pro Asn
    1010                1015                1020                
Gln Pro Lys Thr Asn Tyr Val Asn Leu Arg Ser Tyr Phe Thr Gly Gly
1025                1030                1035                1040
Glu Asn Ile Met Thr Tyr Lys Lys Leu Arg Ile Tyr Ala Ile Thr Pro
                1045                1050                1055    
Asp Asp Arg Glu Leu Leu Val Leu Ser Val Asp
            1060                1065        


<210> 8
<211> 3204
<212> DNA
<213> Artificial Sequence

<220> 
<223> CDTb C37construct. CDTb' (minus pro-domain)
      ligated to Glutathione-S-transferase protein
      polynucleotide

<400> 8
atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt 60
ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa 120
tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat 180
ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac 240
atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg 300
gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt 360
gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa 420
acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat 480
gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa 540
aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca 600
tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat 660
ctggaagttc tgttccaggg gcccctggga tcccatatgg aaattgtgaa tgaagatatt 720
ctgccgaata atggtctgat gggatactac tttaccgatg aacattttaa agatctgaaa 780
ctgatggcac cgattaaaga tggcaatctg aaatttgaag aaaaaaaagt ggataaactg 840
ctggataaag ataaaagtga tgtgaaaagc attcgttgga ccggtcgtat tattccgagc 900
aaagatggtg aatacaccct gagcaccgat cgtgatgatg ttctgatgca ggttaatacc 960
gaaagcacca ttagcaatac cctgaaagtg aatatgaaaa aaggcaaaga atataaagtg 1020
cgcattgaac tgcaggataa aaatctgggt agcattgata atctgagcag cccgaatctg 1080
tattgggaac tggatggtat gaaaaaaatc attccggaag aaaacctgtt tctgcgcgat 1140
tatagcaata ttgaaaaaga tgatccgttt attccgaata ataacttttt tgatccgaaa 1200
ctgatgagcg attgggaaga tgaagatctg gataccgata atgataatat tccggatagc 1260
tatgaacgca atggctatac cattaaagat ctgattgccg tgaaatggga agatagcttt 1320
gcagaacagg gctataagaa atatgtgagc aattatctgg aaagcaatac cgcaggcgat 1380
ccgtataccg attatgaaaa agcaagcggc agctttgata aagccattaa aaccgaagca 1440
cgtgatccgc tggttgcagc atatccgatt gttggtgttg gtatggaaaa actgattatt 1500
agcaccaatg aacatgcaag caccgatcag ggtaaaaccg ttagccgtgc aaccaccaat 1560
agcaaaaccg aaagcaatac agccggtgtt agcgttaatg ttggttatca gaatggtttt 1620
accgccaatg tgaccaccaa ttatagccat accaccgata atagcaccgc agttcaggat 1680
agcaatggtg aaagctggaa taccggtctg agcattaaca aaggtgaaag cgcatatatc 1740
aatgccaatg tgcgctatta taacaccggc accgcaccga tgtataaagt taccccgacc 1800
accaatctgg ttctggatgg tgataccctg agtaccatta aagcacaaga aaatcagatt 1860
ggcaataatc tgagtccggg tgatacctat ccgaaaaaag gtctgagtcc gctggcactg 1920
aataccatgg atcagtttag cagccgtctg attccgatta actatgatca gctgaaaaaa 1980
ctggatgccg gtaaacaaat caaactggaa accacccagg ttagcggtaa ttttggcacc 2040
aaaaattcaa gcggtcagat tgttaccgaa ggtaatagct ggtcagatta tatcagccag 2100
attgatagca ttagcgccag cattattctg gatacagaaa atgaaagcta tgaacgtcgt 2160
gtgaccgcaa aaaatctgca ggacccggaa gataaaacac cggaactgac cattggtgaa 2220
gcaattgaaa aagcatttgg tgccaccaaa aaagatggcc tgctgtattt taacgatatt 2280
ccgattgatg aaagctgcgt ggaactgatt tttgatgata ataccgccaa taaaatcaaa 2340
gatagcctga aaaccctgag cgacaaaaaa atctataatg tgaaactgga acgcggtatg 2400
aatattctga ttaaaacccc gacctatttt accaattttg atgattataa caattatccg 2460
agcacttgga gcaatgtgaa taccaccaat caggatggtc tgcagggtag cgcaaataaa 2520
ctgaatggtg aaaccaaaat caaaattccg atgagcgaac tgaaaccgta taaacgttat 2580
gtgtttagcg gctatagcaa agatccgctg accagcaata gcattattgt gaaaatcaaa 2640
gccaaagaag aaaaaaccga ttatctggtt ccggaacagg gttataccaa atttagctat 2700
gaatttgaaa ccaccgaaaa agatagcagt aatattgaaa ttaccctgat tggtagcggc 2760
accacctatc tggataatct gagtattacc gaactgaata gcacaccgga aattctggat 2820
gaaccggaag tgaaaattcc gaccgatcaa gaaattatgg atgcccataa aatctatttt 2880
gccgatctga actttaatcc gagcaccggc aatacctata ttaacggcat gtattttgca 2940
ccgacccaga ccaataaaga agccctggat tatattcaga aatatcgtgt tgaagccacc 3000
ctgcagtata gcggttttaa agatattggc accaaagata aagaaatgcg taattatctg 3060
ggcgatccga atcagccgaa aaccaattat gttaatctgc gcagctattt taccggtggc 3120
gaaaacatta tgacctacaa aaaactgcgc atttatgcca ttacaccgga tgatcgtgaa 3180
ctgctggttc tgagcgttga ttaa                                        3204

<210> 9
<211> 667
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDTb C40 construct. CDTb'' (minus pro-domain and
      signal peptide) polypeptide

<400> 9
Leu Met Ser Asp Trp Glu Asp Glu Asp Leu Asp Thr Asp Asn Asp Asn
 1               5                  10                  15      
Ile Pro Asp Ser Tyr Glu Arg Asn Gly Tyr Thr Ile Lys Asp Leu Ile
            20                  25                  30          
Ala Val Lys Trp Glu Asp Ser Phe Ala Glu Gln Gly Tyr Lys Lys Tyr
        35                  40                  45              
Val Ser Asn Tyr Leu Glu Ser Asn Thr Ala Gly Asp Pro Tyr Thr Asp
    50                  55                  60                  
Tyr Glu Lys Ala Ser Gly Ser Phe Asp Lys Ala Ile Lys Thr Glu Ala
65                  70                  75                  80  
Arg Asp Pro Leu Val Ala Ala Tyr Pro Ile Val Gly Val Gly Met Glu
                85                  90                  95      
Lys Leu Ile Ile Ser Thr Asn Glu His Ala Ser Thr Asp Gln Gly Lys
            100                 105                 110         
Thr Val Ser Arg Ala Thr Thr Asn Ser Lys Thr Glu Ser Asn Thr Ala
        115                 120                 125             
Gly Val Ser Val Asn Val Gly Tyr Gln Asn Gly Phe Thr Ala Asn Val
    130                 135                 140                 
Thr Thr Asn Tyr Ser His Thr Thr Asp Asn Ser Thr Ala Val Gln Asp
145                 150                 155                 160 
Ser Asn Gly Glu Ser Trp Asn Thr Gly Leu Ser Ile Asn Lys Gly Glu
                165                 170                 175     
Ser Ala Tyr Ile Asn Ala Asn Val Arg Tyr Tyr Asn Thr Gly Thr Ala
            180                 185                 190         
Pro Met Tyr Lys Val Thr Pro Thr Thr Asn Leu Val Leu Asp Gly Asp
        195                 200                 205             
Thr Leu Ser Thr Ile Lys Ala Gln Glu Asn Gln Ile Gly Asn Asn Leu
    210                 215                 220                 
Ser Pro Gly Asp Thr Tyr Pro Lys Lys Gly Leu Ser Pro Leu Ala Leu
225                 230                 235                 240 
Asn Thr Met Asp Gln Phe Ser Ser Arg Leu Ile Pro Ile Asn Tyr Asp
                245                 250                 255     
Gln Leu Lys Lys Leu Asp Ala Gly Lys Gln Ile Lys Leu Glu Thr Thr
            260                 265                 270         
Gln Val Ser Gly Asn Phe Gly Thr Lys Asn Ser Ser Gly Gln Ile Val
        275                 280                 285             
Thr Glu Gly Asn Ser Trp Ser Asp Tyr Ile Ser Gln Ile Asp Ser Ile
    290                 295                 300                 
Ser Ala Ser Ile Ile Leu Asp Thr Glu Asn Glu Ser Tyr Glu Arg Arg
305                 310                 315                 320 
Val Thr Ala Lys Asn Leu Gln Asp Pro Glu Asp Lys Thr Pro Glu Leu
                325                 330                 335     
Thr Ile Gly Glu Ala Ile Glu Lys Ala Phe Gly Ala Thr Lys Lys Asp
            340                 345                 350         
Gly Leu Leu Tyr Phe Asn Asp Ile Pro Ile Asp Glu Ser Cys Val Glu
        355                 360                 365             
Leu Ile Phe Asp Asp Asn Thr Ala Asn Lys Ile Lys Asp Ser Leu Lys
    370                 375                 380                 
Thr Leu Ser Asp Lys Lys Ile Tyr Asn Val Lys Leu Glu Arg Gly Met
385                 390                 395                 400 
Asn Ile Leu Ile Lys Thr Pro Thr Tyr Phe Thr Asn Phe Asp Asp Tyr
                405                 410                 415     
Asn Asn Tyr Pro Ser Thr Trp Ser Asn Val Asn Thr Thr Asn Gln Asp
            420                 425                 430         
Gly Leu Gln Gly Ser Ala Asn Lys Leu Asn Gly Glu Thr Lys Ile Lys
        435                 440                 445             
Ile Pro Met Ser Glu Leu Lys Pro Tyr Lys Arg Tyr Val Phe Ser Gly
    450                 455                 460                 
Tyr Ser Lys Asp Pro Leu Thr Ser Asn Ser Ile Ile Val Lys Ile Lys
465                 470                 475                 480 
Ala Lys Glu Glu Lys Thr Asp Tyr Leu Val Pro Glu Gln Gly Tyr Thr
                485                 490                 495     
Lys Phe Ser Tyr Glu Phe Glu Thr Thr Glu Lys Asp Ser Ser Asn Ile
            500                 505                 510         
Glu Ile Thr Leu Ile Gly Ser Gly Thr Thr Tyr Leu Asp Asn Leu Ser
        515                 520                 525             
Ile Thr Glu Leu Asn Ser Thr Pro Glu Ile Leu Asp Glu Pro Glu Val
    530                 535                 540                 
Lys Ile Pro Thr Asp Gln Glu Ile Met Asp Ala His Lys Ile Tyr Phe
545                 550                 555                 560 
Ala Asp Leu Asn Phe Asn Pro Ser Thr Gly Asn Thr Tyr Ile Asn Gly
                565                 570                 575     
Met Tyr Phe Ala Pro Thr Gln Thr Asn Lys Glu Ala Leu Asp Tyr Ile
            580                 585                 590         
Gln Lys Tyr Arg Val Glu Ala Thr Leu Gln Tyr Ser Gly Phe Lys Asp
        595                 600                 605             
Ile Gly Thr Lys Asp Lys Glu Met Arg Asn Tyr Leu Gly Asp Pro Asn
    610                 615                 620                 
Gln Pro Lys Thr Asn Tyr Val Asn Leu Arg Ser Tyr Phe Thr Gly Gly
625                 630                 635                 640 
Glu Asn Ile Met Thr Tyr Lys Lys Leu Arg Ile Tyr Ala Ile Thr Pro
                645                 650                 655     
Asp Asp Arg Glu Leu Leu Val Leu Ser Val Asp
            660                 665         


<210> 10
<211> 420
<212> PRT
<213> Artificial Sequence

<220> 
<223> C44 construct. CDTa mutation E428Q polypeptide

<400> 10
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Ser Leu Asp Phe Lys Asp Asp
    210                 215                 220                 
Val Ser Lys Gly Asp Ser Trp Gly Lys Ala Asn Tyr Asn Asp Trp Ser
225                 230                 235                 240 
Asn Lys Leu Thr Pro Asn Glu Leu Ala Asp Val Asn Asp Tyr Met Arg
                245                 250                 255     
Gly Gly Tyr Thr Ala Ile Asn Asn Tyr Leu Ile Ser Asn Gly Pro Val
            260                 265                 270         
Asn Asn Pro Asn Pro Glu Leu Asp Ser Lys Ile Thr Asn Ile Glu Asn
        275                 280                 285             
Ala Leu Lys Arg Glu Pro Ile Pro Thr Asn Leu Thr Val Tyr Arg Arg
    290                 295                 300                 
Ser Gly Pro Gln Glu Phe Gly Leu Thr Leu Thr Ser Pro Glu Tyr Asp
305                 310                 315                 320 
Phe Asn Lys Leu Glu Asn Ile Asp Ala Phe Lys Ser Lys Trp Glu Gly
                325                 330                 335     
Gln Ala Leu Ser Tyr Pro Asn Phe Ile Ser Thr Ser Ile Gly Ser Val
            340                 345                 350         
Asn Met Ser Ala Phe Ala Lys Arg Lys Ile Val Leu Arg Ile Thr Ile
        355                 360                 365             
Pro Lys Gly Ser Pro Gly Ala Tyr Leu Ser Ala Ile Pro Gly Tyr Ala
    370                 375                 380                 
Gly Tyr Glu Val Leu Leu Asn His Gly Ser Lys Phe Lys Ile Asn Lys
385                 390                 395                 400 
Ile Asp Ser Tyr Lys Asp Gly Thr Ile Thr Lys Leu Ile Val Asp Ala
                405                 410                 415     
Thr Leu Ile Pro
            420 


<210> 11
<211> 1266
<212> DNA
<213> Artificial Sequence

<220> 
<223> CDTa mutation E428Q polynucleotide

<400> 11
atggtttgca ataccaccta taaagcaccg attgaacgtc cggaagattt tctgaaagat 60
aaagaaaaag ccaaagaatg ggaacgcaaa gaagcagaac gtattgaaca gaaactggaa 120
cgtagcgaaa aagaagcact ggaaagctac aaaaaagata gcgtggaaat ttcaaaatat 180
agccagaccc gcaattattt ctatgattat cagattgaag ccaatagccg tgaaaaagaa 240
tataaagaac tgcgcaatgc cattagcaaa aacaaaattg ataaaccgat gtatgtgtat 300
tatttcgaaa gtccggaaaa atttgccttt aacaaagtga ttcgcaccga aaatcagaat 360
gaaattagcc tggaaaaatt caatgaattt aaagaaacca ttcagaataa actgtttaaa 420
caggatggct ttaaagatat ttcactgtat gaaccgggta aaggtgatga aaaaccgaca 480
ccgctgctga tgcatctgaa actgcctcgt aataccggta tgctgccgta taccaatacc 540
aataatgtta gcaccctgat tgaacagggc tatagcatca aaattgataa aattgtgcgc 600
attgtgattg atggcaaaca ttatatcaaa gccgaagcca gcgttgtttc aagcctggat 660
tttaaagatg atgtgagcaa aggcgatagc tggggtaaag caaactataa tgattggagc 720
aataaactga ccccgaatga actggcagat gtgaatgatt atatgcgtgg tggttatacc 780
gccattaaca attatctgat tagcaatggt ccggtgaata atccgaatcc ggaactggat 840
agcaaaatta ccaatattga aaatgccctg aaacgcgaac cgattccgac caatctgacc 900
gtttatcgtc gtagcggtcc gcaagaattt ggtctgaccc tgaccagtcc ggaatatgac 960
tttaacaaac tggaaaatat tgatgccttt aaaagcaaat gggaaggtca ggcactgagc 1020
tatccgaact ttattagcac cagcattggt agcgttaata tgagcgcatt tgccaaacgt 1080
aaaattgtgc tgcgtattac cattccgaaa ggtagtccgg gtgcatatct gagcgcaatt 1140
ccgggttatg ccggtcaata tgaagttctg ctgaatcatg gcagcaaatt caaaattaac 1200
aaaattgata gctataaaga tggcaccatt accaaactga ttgttgatgc aaccctgatt 1260
ccgtaa                                                            1266

<210> 12
<211> 420
<212> PRT
<213> Artificial Sequence

<220> 
<223> C54 construct. CDTa mutation E430Q polypeptide

<400> 12
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Ser Leu Asp Phe Lys Asp Asp
    210                 215                 220                 
Val Ser Lys Gly Asp Ser Trp Gly Lys Ala Asn Tyr Asn Asp Trp Ser
225                 230                 235                 240 
Asn Lys Leu Thr Pro Asn Glu Leu Ala Asp Val Asn Asp Tyr Met Arg
                245                 250                 255     
Gly Gly Tyr Thr Ala Ile Asn Asn Tyr Leu Ile Ser Asn Gly Pro Val
            260                 265                 270         
Asn Asn Pro Asn Pro Glu Leu Asp Ser Lys Ile Thr Asn Ile Glu Asn
        275                 280                 285             
Ala Leu Lys Arg Glu Pro Ile Pro Thr Asn Leu Thr Val Tyr Arg Arg
    290                 295                 300                 
Ser Gly Pro Gln Glu Phe Gly Leu Thr Leu Thr Ser Pro Glu Tyr Asp
305                 310                 315                 320 
Phe Asn Lys Leu Glu Asn Ile Asp Ala Phe Lys Ser Lys Trp Glu Gly
                325                 330                 335     
Gln Ala Leu Ser Tyr Pro Asn Phe Ile Ser Thr Ser Ile Gly Ser Val
            340                 345                 350         
Asn Met Ser Ala Phe Ala Lys Arg Lys Ile Val Leu Arg Ile Thr Ile
        355                 360                 365             
Pro Lys Gly Ser Pro Gly Ala Tyr Leu Ser Ala Ile Pro Gly Tyr Ala
    370                 375                 380                 
Gly Glu Tyr Val Leu Leu Asn His Gly Ser Lys Phe Lys Ile Asn Lys
385                 390                 395                 400 
Ile Asp Ser Tyr Lys Asp Gly Thr Ile Thr Lys Leu Ile Val Asp Ala
                405                 410                 415     
Thr Leu Ile Pro
            420 


<210> 13
<211> 198
<212> PRT
<213> Artificial Sequence

<220> 
<223> CDTa N terminal domain (residue 44 to residue 240)
      polypeptide

<400> 13
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile
        195             


<210> 14
<211> 226
<212> PRT
<213> Artificial Sequence

<220> 
<223> C49 construct. CDTa Nterminal domain without
      signal peptide, with the linker existing between
      the N-term domain and the C-term domain
      (containing the enzymatic activity). This
      construct covers the fragment from amino acid 44
      to aa 268 polypeptide sequence.

<400> 14
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Ser Leu Asp Phe Lys Asp Asp
    210                 215                 220                 
Val Ser
225     


<210> 15
<211> 218
<212> PRT
<213> Artificial Sequence

<220> 
<223> C50 construct. CDTa without signal peptide and the
      linker existing between the Nterminal and
      Cterminal domains of CDTa. This construct covers
      the fragment from aa 44 to aa 260 polypeptide
      sequence.

<400> 15
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Ser
    210                 215             


<210> 16
<211> 834
<212> PRT
<213> Artificial Sequence

<220> 
<223> Polypeptide sequence of CDTb with pro-domain
      removed (CDTb')

<400> 16
Glu Ile Val Asn Glu Asp Ile Leu Pro Asn Asn Gly Leu Met Gly Tyr
 1               5                  10                  15      
Tyr Phe Thr Asp Glu His Phe Lys Asp Leu Lys Leu Met Ala Pro Ile
            20                  25                  30          
Lys Asp Gly Asn Leu Lys Phe Glu Glu Lys Lys Val Asp Lys Leu Leu
        35                  40                  45              
Asp Lys Asp Lys Ser Asp Val Lys Ser Ile Arg Trp Thr Gly Arg Ile
    50                  55                  60                  
Ile Pro Ser Lys Asp Gly Glu Tyr Thr Leu Ser Thr Asp Arg Asp Asp
65                  70                  75                  80  
Val Leu Met Gln Val Asn Thr Glu Ser Thr Ile Ser Asn Thr Leu Lys
                85                  90                  95      
Val Asn Met Lys Lys Gly Lys Glu Tyr Lys Val Arg Ile Glu Leu Gln
            100                 105                 110         
Asp Lys Asn Leu Gly Ser Ile Asp Asn Leu Ser Ser Pro Asn Leu Tyr
        115                 120                 125             
Trp Glu Leu Asp Gly Met Lys Lys Ile Ile Pro Glu Glu Asn Leu Phe
    130                 135                 140                 
Leu Arg Asp Tyr Ser Asn Ile Glu Lys Asp Asp Pro Phe Ile Pro Asn
145                 150                 155                 160 
Asn Asn Phe Phe Asp Pro Lys Leu Met Ser Asp Trp Glu Asp Glu Asp
                165                 170                 175     
Leu Asp Thr Asp Asn Asp Asn Ile Pro Asp Ser Tyr Glu Arg Asn Gly
            180                 185                 190         
Tyr Thr Ile Lys Asp Leu Ile Ala Val Lys Trp Glu Asp Ser Phe Ala
        195                 200                 205             
Glu Gln Gly Tyr Lys Lys Tyr Val Ser Asn Tyr Leu Glu Ser Asn Thr
    210                 215                 220                 
Ala Gly Asp Pro Tyr Thr Asp Tyr Glu Lys Ala Ser Gly Ser Phe Asp
225                 230                 235                 240 
Lys Ala Ile Lys Thr Glu Ala Arg Asp Pro Leu Val Ala Ala Tyr Pro
                245                 250                 255     
Ile Val Gly Val Gly Met Glu Lys Leu Ile Ile Ser Thr Asn Glu His
            260                 265                 270         
Ala Ser Thr Asp Gln Gly Lys Thr Val Ser Arg Ala Thr Thr Asn Ser
        275                 280                 285             
Lys Thr Glu Ser Asn Thr Ala Gly Val Ser Val Asn Val Gly Tyr Gln
    290                 295                 300                 
Asn Gly Phe Thr Ala Asn Val Thr Thr Asn Tyr Ser His Thr Thr Asp
305                 310                 315                 320 
Asn Ser Thr Ala Val Gln Asp Ser Asn Gly Glu Ser Trp Asn Thr Gly
                325                 330                 335     
Leu Ser Ile Asn Lys Gly Glu Ser Ala Tyr Ile Asn Ala Asn Val Arg
            340                 345                 350         
Tyr Tyr Asn Thr Gly Thr Ala Pro Met Tyr Lys Val Thr Pro Thr Thr
        355                 360                 365             
Asn Leu Val Leu Asp Gly Asp Thr Leu Ser Thr Ile Lys Ala Gln Glu
    370                 375                 380                 
Asn Gln Ile Gly Asn Asn Leu Ser Pro Gly Asp Thr Tyr Pro Lys Lys
385                 390                 395                 400 
Gly Leu Ser Pro Leu Ala Leu Asn Thr Met Asp Gln Phe Ser Ser Arg
                405                 410                 415     
Leu Ile Pro Ile Asn Tyr Asp Gln Leu Lys Lys Leu Asp Ala Gly Lys
            420                 425                 430         
Gln Ile Lys Leu Glu Thr Thr Gln Val Ser Gly Asn Phe Gly Thr Lys
        435                 440                 445             
Asn Ser Ser Gly Gln Ile Val Thr Glu Gly Asn Ser Trp Ser Asp Tyr
    450                 455                 460                 
Ile Ser Gln Ile Asp Ser Ile Ser Ala Ser Ile Ile Leu Asp Thr Glu
465                 470                 475                 480 
Asn Glu Ser Tyr Glu Arg Arg Val Thr Ala Lys Asn Leu Gln Asp Pro
                485                 490                 495     
Glu Asp Lys Thr Pro Glu Leu Thr Ile Gly Glu Ala Ile Glu Lys Ala
            500                 505                 510         
Phe Gly Ala Thr Lys Lys Asp Gly Leu Leu Tyr Phe Asn Asp Ile Pro
        515                 520                 525             
Ile Asp Glu Ser Cys Val Glu Leu Ile Phe Asp Asp Asn Thr Ala Asn
    530                 535                 540                 
Lys Ile Lys Asp Ser Leu Lys Thr Leu Ser Asp Lys Lys Ile Tyr Asn
545                 550                 555                 560 
Val Lys Leu Glu Arg Gly Met Asn Ile Leu Ile Lys Thr Pro Thr Tyr
                565                 570                 575     
Phe Thr Asn Phe Asp Asp Tyr Asn Asn Tyr Pro Ser Thr Trp Ser Asn
            580                 585                 590         
Val Asn Thr Thr Asn Gln Asp Gly Leu Gln Gly Ser Ala Asn Lys Leu
        595                 600                 605             
Asn Gly Glu Thr Lys Ile Lys Ile Pro Met Ser Glu Leu Lys Pro Tyr
    610                 615                 620                 
Lys Arg Tyr Val Phe Ser Gly Tyr Ser Lys Asp Pro Leu Thr Ser Asn
625                 630                 635                 640 
Ser Ile Ile Val Lys Ile Lys Ala Lys Glu Glu Lys Thr Asp Tyr Leu
                645                 650                 655     
Val Pro Glu Gln Gly Tyr Thr Lys Phe Ser Tyr Glu Phe Glu Thr Thr
            660                 665                 670         
Glu Lys Asp Ser Ser Asn Ile Glu Ile Thr Leu Ile Gly Ser Gly Thr
        675                 680                 685             
Thr Tyr Leu Asp Asn Leu Ser Ile Thr Glu Leu Asn Ser Thr Pro Glu
    690                 695                 700                 
Ile Leu Asp Glu Pro Glu Val Lys Ile Pro Thr Asp Gln Glu Ile Met
705                 710                 715                 720 
Asp Ala His Lys Ile Tyr Phe Ala Asp Leu Asn Phe Asn Pro Ser Thr
                725                 730                 735     
Gly Asn Thr Tyr Ile Asn Gly Met Tyr Phe Ala Pro Thr Gln Thr Asn
            740                 745                 750         
Lys Glu Ala Leu Asp Tyr Ile Gln Lys Tyr Arg Val Glu Ala Thr Leu
        755                 760                 765             
Gln Tyr Ser Gly Phe Lys Asp Ile Gly Thr Lys Asp Lys Glu Met Arg
    770                 775                 780                 
Asn Tyr Leu Gly Asp Pro Asn Gln Pro Lys Thr Asn Tyr Val Asn Leu
785                 790                 795                 800 
Arg Ser Tyr Phe Thr Gly Gly Glu Asn Ile Met Thr Tyr Lys Lys Leu
                805                 810                 815     
Arg Ile Tyr Ala Ile Thr Pro Asp Asp Arg Glu Leu Leu Val Leu Ser
            820                 825                 830         
Val Asp
        


<210> 17
<211> 2511
<212> DNA
<213> Artificial Sequence

<220> 
<223> Polypeptide sequence of CDTb with pro-domain
      removed (CDTb')

<400> 17
catatggaaa ttgtgaatga agatattctg ccgaataatg gtctgatggg atactacttt 60
accgatgaac attttaaaga tctgaaactg atggcaccga ttaaagatgg caatctgaaa 120
tttgaagaaa aaaaagtgga taaactgctg gataaagata aaagtgatgt gaaaagcatt 180
cgttggaccg gtcgtattat tccgagcaaa gatggtgaat acaccctgag caccgatcgt 240
gatgatgttc tgatgcaggt taataccgaa agcaccatta gcaataccct gaaagtgaat 300
atgaaaaaag gcaaagaata taaagtgcgc attgaactgc aggataaaaa tctgggtagc 360
attgataatc tgagcagccc gaatctgtat tgggaactgg atggtatgaa aaaaatcatt 420
ccggaagaaa acctgtttct gcgcgattat agcaatattg aaaaagatga tccgtttatt 480
ccgaataata acttttttga tccgaaactg atgagcgatt gggaagatga agatctggat 540
accgataatg ataatattcc ggatagctat gaacgcaatg gctataccat taaagatctg 600
attgccgtga aatgggaaga tagctttgca gaacagggct ataagaaata tgtgagcaat 660
tatctggaaa gcaataccgc aggcgatccg tataccgatt atgaaaaagc aagcggcagc 720
tttgataaag ccattaaaac cgaagcacgt gatccgctgg ttgcagcata tccgattgtt 780
ggtgttggta tggaaaaact gattattagc accaatgaac atgcaagcac cgatcagggt 840
aaaaccgtta gccgtgcaac caccaatagc aaaaccgaaa gcaatacagc cggtgttagc 900
gttaatgttg gttatcagaa tggttttacc gccaatgtga ccaccaatta tagccatacc 960
accgataata gcaccgcagt tcaggatagc aatggtgaaa gctggaatac cggtctgagc 1020
attaacaaag gtgaaagcgc atatatcaat gccaatgtgc gctattataa caccggcacc 1080
gcaccgatgt ataaagttac cccgaccacc aatctggttc tggatggtga taccctgagt 1140
accattaaag cacaagaaaa tcagattggc aataatctga gtccgggtga tacctatccg 1200
aaaaaaggtc tgagtccgct ggcactgaat accatggatc agtttagcag ccgtctgatt 1260
ccgattaact atgatcagct gaaaaaactg gatgccggta aacaaatcaa actggaaacc 1320
acccaggtta gcggtaattt tggcaccaaa aattcaagcg gtcagattgt taccgaaggt 1380
aatagctggt cagattatat cagccagatt gatagcatta gcgccagcat tattctggat 1440
acagaaaatg aaagctatga acgtcgtgtg accgcaaaaa atctgcagga cccggaagat 1500
aaaacaccgg aactgaccat tggtgaagca attgaaaaag catttggtgc caccaaaaaa 1560
gatggcctgc tgtattttaa cgatattccg attgatgaaa gctgcgtgga actgattttt 1620
gatgataata ccgccaataa aatcaaagat agcctgaaaa ccctgagcga caaaaaaatc 1680
tataatgtga aactggaacg cggtatgaat attctgatta aaaccccgac ctattttacc 1740
aattttgatg attataacaa ttatccgagc acttggagca atgtgaatac caccaatcag 1800
gatggtctgc agggtagcgc aaataaactg aatggtgaaa ccaaaatcaa aattccgatg 1860
agcgaactga aaccgtataa acgttatgtg tttagcggct atagcaaaga tccgctgacc 1920
agcaatagca ttattgtgaa aatcaaagcc aaagaagaaa aaaccgatta tctggttccg 1980
gaacagggtt ataccaaatt tagctatgaa tttgaaacca ccgaaaaaga tagcagtaat 2040
attgaaatta ccctgattgg tagcggcacc acctatctgg ataatctgag tattaccgaa 2100
ctgaatagca caccggaaat tctggatgaa ccggaagtga aaattccgac cgatcaagaa 2160
attatggatg cccataaaat ctattttgcc gatctgaact ttaatccgag caccggcaat 2220
acctatatta acggcatgta ttttgcaccg acccagacca ataaagaagc cctggattat 2280
attcagaaat atcgtgttga agccaccctg cagtatagcg gttttaaaga tattggcacc 2340
aaagataaag aaatgcgtaa ttatctgggc gatccgaatc agccgaaaac caattatgtt 2400
aatctgcgca gctattttac cggtggcgaa aacattatga cctacaaaaa actgcgcatt 2460
tatgccatta caccggatga tcgtgaactg ctggttctga gcgttgatta a          2511

<210> 18
<211> 966
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of Fusion 1 (F1)

<400> 18
Met Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn
 1               5                  10                  15      
Thr Ala Ile Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe
            20                  25                  30          
Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro
        35                  40                  45              
Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile
    50                  55                  60                  
Glu Gly Gln Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly
65                  70                  75                  80  
Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg
                85                  90                  95      
Ile Ile Asn Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala
            100                 105                 110         
Ala Ile His Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr
        115                 120                 125             
Asp Gly Ile Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe
    130                 135                 140                 
Tyr Phe Asp Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys
145                 150                 155                 160 
Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn
                165                 170                 175     
Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu
            180                 185                 190         
Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly
        195                 200                 205             
Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala
    210                 215                 220                 
Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe
225                 230                 235                 240 
Asn Leu Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly
                245                 250                 255     
Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr
            260                 265                 270         
Thr Ser Ile Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met
        275                 280                 285             
Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro
    290                 295                 300                 
Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln
305                 310                 315                 320 
Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp
                325                 330                 335     
Ser Lys Ala Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr
            340                 345                 350         
Phe Asn Thr Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn
        355                 360                 365             
Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly
    370                 375                 380                 
Tyr Thr Ile Ile Ser Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile
385                 390                 395                 400 
Met Gln Ile Gly Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr Phe Ala
                405                 410                 415     
Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr
            420                 425                 430         
Gln Asn Arg Phe Leu Tyr Leu His Asp Asn Ile Tyr Tyr Phe Gly Asn
        435                 440                 445             
Asn Ser Lys Ala Ala Thr Gly Trp Val Thr Ile Asp Gly Asn Arg Tyr
    450                 455                 460                 
Tyr Phe Glu Pro Asn Thr Ala Met Gly Ala Asn Gly Tyr Lys Thr Ile
465                 470                 475                 480 
Asp Asn Lys Asn Phe Tyr Phe Arg Asn Gly Leu Pro Gln Ile Gly Val
                485                 490                 495     
Phe Lys Gly Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp
            500                 505                 510         
Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg Phe Leu
        515                 520                 525             
His Leu Leu Gly Lys Ile Tyr Tyr Phe Gly Asn Asn Ser Lys Ala Val
    530                 535                 540                 
Thr Gly Trp Gln Thr Ile Asn Gly Lys Val Tyr Tyr Phe Met Pro Asp
545                 550                 555                 560 
Thr Ala Met Ala Ala Ala Gly Gly Leu Phe Glu Ile Asp Gly Val Ile
                565                 570                 575     
Tyr Phe Phe Gly Val Asp Gly Val Lys Ala Pro Gly Phe Val Ser Ile
            580                 585                 590         
Asn Asp Asn Lys His Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly
        595                 600                 605             
Tyr Thr Glu Ile Asp Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu
    610                 615                 620                 
Met Gln Ile Gly Val Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala
625                 630                 635                 640 
His His Asn Glu Asp Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr
                645                 650                 655     
Ser Gly Ile Leu Asn Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser
            660                 665                 670         
Phe Thr Ala Val Val Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr
        675                 680                 685             
Tyr Phe Asp Glu Asp Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile
    690                 695                 700                 
Asn Asp Gly Gln Tyr Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly
705                 710                 715                 720 
Phe Val Thr Ile Asn Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile
                725                 730                 735     
Ile Glu Ser Gly Val Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp
            740                 745                 750         
Asp Asn Gly Ile Val Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr
        755                 760                 765             
Lys Tyr Phe Ala Pro Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln
    770                 775                 780                 
Ala Val Glu Tyr Ser Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr
785                 790                 795                 800 
Phe Gly Glu Thr Tyr Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu
                805                 810                 815     
Asn Glu Ser Asp Lys Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys
            820                 825                 830         
Lys Gly Ile Asn Leu Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys
        835                 840                 845             
Gly Ile Met Arg Thr Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr
    850                 855                 860                 
Phe Asn Glu Asn Gly Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp
865                 870                 875                 880 
Lys Met Phe Tyr Phe Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe
                885                 890                 895     
Asn Thr Pro Asp Gly Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp
            900                 905                 910         
Glu Asn Phe Glu Gly Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu
        915                 920                 925             
Asp Glu Lys Arg Tyr Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly
    930                 935                 940                 
Ser Val Ile Ile Asp Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala
945                 950                 955                 960 
Gln Leu Val Ile Ser Glu
                965     


<210> 19
<211> 966
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of Fusion 2 (F2)

<400> 19
Met Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn
 1               5                  10                  15      
Thr Ala Ile Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe
            20                  25                  30          
Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro
        35                  40                  45              
Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile
    50                  55                  60                  
Glu Gly Gln Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly
65                  70                  75                  80  
Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg
                85                  90                  95      
Ile Ile Asn Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala
            100                 105                 110         
Ala Ile His Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr
        115                 120                 125             
Asp Gly Ile Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe
    130                 135                 140                 
Tyr Phe Asp Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys
145                 150                 155                 160 
Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn
                165                 170                 175     
Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu
            180                 185                 190         
Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly
        195                 200                 205             
Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala
    210                 215                 220                 
Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe
225                 230                 235                 240 
Asn Leu Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly
                245                 250                 255     
Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr
            260                 265                 270         
Thr Ser Ile Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met
        275                 280                 285             
Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro
    290                 295                 300                 
Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln
305                 310                 315                 320 
Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp
                325                 330                 335     
Ser Lys Ala Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr
            340                 345                 350         
Phe Asn Thr Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn
        355                 360                 365             
Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly
    370                 375                 380                 
Tyr Thr Ile Ile Ser Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile
385                 390                 395                 400 
Met Gln Ile Gly Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr Phe Ala
                405                 410                 415     
Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr
            420                 425                 430         
Gln Asn Arg Phe Leu Tyr Leu His Asp Asn Ile Tyr Tyr Phe Gly Asn
        435                 440                 445             
Asn Ser Lys Ala Ala Thr Gly Trp Val Thr Ile Asp Gly Asn Arg Tyr
    450                 455                 460                 
Tyr Phe Glu Pro Asn Thr Ala Met Gly Ala Asn Gly Tyr Lys Thr Ile
465                 470                 475                 480 
Asp Asn Lys Asn Phe Tyr Phe Arg Asn Gly Leu Pro Gln Ile Gly Val
                485                 490                 495     
Phe Lys Gly Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp
            500                 505                 510         
Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg Phe Leu
        515                 520                 525             
His Leu Leu Gly Lys Ile Tyr Tyr Phe Gly Asn Asn Ser Lys Ala Val
    530                 535                 540                 
Thr Gly Trp Gln Thr Ile Asn Gly Lys Val Tyr Tyr Phe Met Pro Asp
545                 550                 555                 560 
Thr Ala Met Ala Ala Ala Gly Gly Leu Asn Gln Ile Gly Asp Tyr Lys
                565                 570                 575     
Tyr Tyr Phe Asn Ser Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile
            580                 585                 590         
Asn Asp Asn Lys His Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly
        595                 600                 605             
Tyr Thr Glu Ile Asp Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu
    610                 615                 620                 
Met Gln Ile Gly Val Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala
625                 630                 635                 640 
His His Asn Glu Asp Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr
                645                 650                 655     
Ser Gly Ile Leu Asn Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser
            660                 665                 670         
Phe Thr Ala Val Val Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr
        675                 680                 685             
Tyr Phe Asp Glu Asp Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile
    690                 695                 700                 
Asn Asp Gly Gln Tyr Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly
705                 710                 715                 720 
Phe Val Thr Ile Asn Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile
                725                 730                 735     
Ile Glu Ser Gly Val Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp
            740                 745                 750         
Asp Asn Gly Ile Val Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr
        755                 760                 765             
Lys Tyr Phe Ala Pro Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln
    770                 775                 780                 
Ala Val Glu Tyr Ser Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr
785                 790                 795                 800 
Phe Gly Glu Thr Tyr Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu
                805                 810                 815     
Asn Glu Ser Asp Lys Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys
            820                 825                 830         
Lys Gly Ile Asn Leu Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys
        835                 840                 845             
Gly Ile Met Arg Thr Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr
    850                 855                 860                 
Phe Asn Glu Asn Gly Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp
865                 870                 875                 880 
Lys Met Phe Tyr Phe Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe
                885                 890                 895     
Asn Thr Pro Asp Gly Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp
            900                 905                 910         
Glu Asn Phe Glu Gly Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu
        915                 920                 925             
Asp Glu Lys Arg Tyr Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly
    930                 935                 940                 
Ser Val Ile Ile Asp Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala
945                 950                 955                 960 
Gln Leu Val Ile Ser Glu
                965     


<210> 20
<211> 833
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of Fusion 3 (F3)

<400> 20
Met Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn
 1               5                  10                  15      
Thr Ala Ile Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe
            20                  25                  30          
Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro
        35                  40                  45              
Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile
    50                  55                  60                  
Glu Gly Gln Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly
65                  70                  75                  80  
Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg
                85                  90                  95      
Ile Ile Asn Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala
            100                 105                 110         
Ala Ile His Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr
        115                 120                 125             
Asp Gly Ile Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe
    130                 135                 140                 
Tyr Phe Asp Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys
145                 150                 155                 160 
Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn
                165                 170                 175     
Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu
            180                 185                 190         
Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly
        195                 200                 205             
Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala
    210                 215                 220                 
Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe
225                 230                 235                 240 
Asn Leu Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly
                245                 250                 255     
Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr
            260                 265                 270         
Thr Ser Ile Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met
        275                 280                 285             
Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro
    290                 295                 300                 
Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln
305                 310                 315                 320 
Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp
                325                 330                 335     
Ser Lys Ala Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr
            340                 345                 350         
Phe Asn Thr Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn
        355                 360                 365             
Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly
    370                 375                 380                 
Tyr Thr Ile Ile Ser Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile
385                 390                 395                 400 
Met Gln Ile Gly Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr Phe Ala
                405                 410                 415     
Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr
            420                 425                 430         
Gln Asn Arg Phe Leu Tyr Leu His Asp Asn Ile Tyr Tyr Phe Gly Asn
        435                 440                 445             
Asn Ser Lys Ala Ala Thr Gly Trp Val Thr Ile Asp Gly Asn Arg Tyr
    450                 455                 460                 
Tyr Phe Glu Pro Asn Thr Ala Met Gly Ala Asn Gly Tyr Lys Thr Ile
465                 470                 475                 480 
Asp Asn Lys Asn Phe Tyr Phe Arg Asn Gly Leu Pro Gln Ile Gly Val
                485                 490                 495     
Phe Lys Gly Ser Asn Gly Phe Glu Tyr Phe Ala His His Asn Glu Asp
            500                 505                 510         
Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile Leu Asn
        515                 520                 525             
Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala Val Val
    530                 535                 540                 
Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp Glu Asp
545                 550                 555                 560 
Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly Gln Tyr
                565                 570                 575     
Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly Phe Val Thr Ile Asn
            580                 585                 590         
Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser Gly Val
        595                 600                 605             
Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly Ile Val
    610                 615                 620                 
Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe Ala Pro
625                 630                 635                 640 
Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu Tyr Ser
                645                 650                 655     
Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu Thr Tyr
            660                 665                 670         
Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser Asp Lys
        675                 680                 685             
Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile Asn Leu
    690                 695                 700                 
Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met Arg Thr
705                 710                 715                 720 
Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu Asn Gly
                725                 730                 735     
Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe Tyr Phe
            740                 745                 750         
Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe Asn Thr Pro Asp Gly
        755                 760                 765             
Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe Glu Gly
    770                 775                 780                 
Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys Arg Tyr
785                 790                 795                 800 
Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile Ile Asp
                805                 810                 815     
Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val Ile Ser
            820                 825                 830         
Glu
    


<210> 21
<211> 1057
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of Fusion 4 (F4)

<400> 21
Met Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn
 1               5                  10                  15      
Thr Ala Ile Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe
            20                  25                  30          
Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro
        35                  40                  45              
Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile
    50                  55                  60                  
Glu Gly Gln Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly
65                  70                  75                  80  
Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg
                85                  90                  95      
Ile Ile Asn Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala
            100                 105                 110         
Ala Ile His Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr
        115                 120                 125             
Asp Gly Ile Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe
    130                 135                 140                 
Tyr Phe Asp Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys
145                 150                 155                 160 
Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn
                165                 170                 175     
Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu
            180                 185                 190         
Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly
        195                 200                 205             
Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala
    210                 215                 220                 
Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe
225                 230                 235                 240 
Asn Leu Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly
                245                 250                 255     
Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr
            260                 265                 270         
Thr Ser Ile Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met
        275                 280                 285             
Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro
    290                 295                 300                 
Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln
305                 310                 315                 320 
Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp
                325                 330                 335     
Ser Lys Ala Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr
            340                 345                 350         
Phe Asn Thr Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn
        355                 360                 365             
Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly
    370                 375                 380                 
Tyr Thr Ile Ile Ser Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile
385                 390                 395                 400 
Met Gln Ile Gly Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr Phe Ala
                405                 410                 415     
Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr
            420                 425                 430         
Gln Asn Arg Phe Leu Tyr Leu His Asp Asn Ile Tyr Tyr Phe Gly Asn
        435                 440                 445             
Asn Ser Lys Ala Ala Thr Gly Trp Val Thr Ile Asp Gly Asn Arg Tyr
    450                 455                 460                 
Tyr Phe Glu Pro Asn Thr Ala Met Gly Ala Asn Gly Tyr Lys Thr Ile
465                 470                 475                 480 
Asp Asn Lys Asn Phe Tyr Phe Arg Asn Gly Leu Pro Gln Ile Gly Val
                485                 490                 495     
Phe Lys Gly Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp
            500                 505                 510         
Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg Phe Leu
        515                 520                 525             
His Leu Leu Gly Lys Ile Tyr Tyr Phe Gly Asn Asn Ser Lys Ala Val
    530                 535                 540                 
Thr Gly Trp Gln Thr Ile Asn Gly Lys Val Tyr Tyr Phe Met Pro Asp
545                 550                 555                 560 
Thr Ala Met Ala Ala Ala Gly Gly Glu Thr Ile Ile Asp Asp Lys Asn
                565                 570                 575     
Tyr Tyr Phe Asn Gln Ser Gly Val Leu Gln Thr Gly Val Phe Ser Thr
            580                 585                 590         
Glu Asp Gly Phe Lys Tyr Phe Ala Pro Ala Asn Thr Leu Asp Glu Asn
        595                 600                 605             
Leu Glu Gly Glu Ala Ile Asp Phe Thr Gly Lys Leu Ile Ile Asp Glu
    610                 615                 620                 
Asn Ile Tyr Tyr Phe Asp Asp Asn Tyr Arg Gly Ala Val Glu Trp Lys
625                 630                 635                 640 
Glu Leu Asp Gly Glu Met His Tyr Phe Ser Pro Glu Thr Gly Lys Ala
                645                 650                 655     
Phe Lys Gly Leu Asn Gln Ile Gly Asp Tyr Lys Tyr Tyr Phe Asn Ser
            660                 665                 670         
Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile Asn Asp Asn Lys His
        675                 680                 685             
Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly Tyr Thr Glu Ile Asp
    690                 695                 700                 
Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile Gly Val
705                 710                 715                 720 
Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala His His Asn Glu Asp
                725                 730                 735     
Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile Leu Asn
            740                 745                 750         
Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala Val Val
        755                 760                 765             
Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp Glu Asp
    770                 775                 780                 
Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly Gln Tyr
785                 790                 795                 800 
Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly Phe Val Thr Ile Asn
                805                 810                 815     
Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser Gly Val
            820                 825                 830         
Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly Ile Val
        835                 840                 845             
Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe Ala Pro
    850                 855                 860                 
Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu Tyr Ser
865                 870                 875                 880 
Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu Thr Tyr
                885                 890                 895     
Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser Asp Lys
            900                 905                 910         
Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile Asn Leu
        915                 920                 925             
Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met Arg Thr
    930                 935                 940                 
Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu Asn Gly
945                 950                 955                 960 
Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe Tyr Phe
                965                 970                 975     
Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe Asn Thr Pro Asp Gly
            980                 985                 990         
Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe Glu Gly
        995                 1000                1005            
Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys Arg Tyr
    1010                1015                1020                
Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile Ile Asp
1025                1030                1035                1040
Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val Ile Ser
                1045                1050                1055    
Glu
    


<210> 22
<211> 971
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of Fusion 5 (F5)

<400> 22
Met Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn
 1               5                  10                  15      
Thr Ala Ile Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe
            20                  25                  30          
Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro
        35                  40                  45              
Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile
    50                  55                  60                  
Glu Gly Gln Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly
65                  70                  75                  80  
Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg
                85                  90                  95      
Ile Ile Asn Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala
            100                 105                 110         
Ala Ile His Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr
        115                 120                 125             
Asp Gly Ile Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe
    130                 135                 140                 
Tyr Phe Asp Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys
145                 150                 155                 160 
Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn
                165                 170                 175     
Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu
            180                 185                 190         
Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly
        195                 200                 205             
Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala
    210                 215                 220                 
Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe
225                 230                 235                 240 
Asn Leu Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly
                245                 250                 255     
Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr
            260                 265                 270         
Thr Ser Ile Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met
        275                 280                 285             
Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro
    290                 295                 300                 
Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln
305                 310                 315                 320 
Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp
                325                 330                 335     
Ser Lys Ala Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr
            340                 345                 350         
Phe Asn Thr Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn
        355                 360                 365             
Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly
    370                 375                 380                 
Tyr Thr Ile Ile Ser Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile
385                 390                 395                 400 
Met Gln Ile Gly Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr Phe Ala
                405                 410                 415     
Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr
            420                 425                 430         
Gln Asn Arg Phe Leu Tyr Leu His Asp Asn Ile Tyr Tyr Phe Gly Asn
        435                 440                 445             
Asn Ser Lys Ala Ala Thr Gly Trp Val Thr Ile Asp Gly Asn Arg Tyr
    450                 455                 460                 
Tyr Phe Glu Pro Asn Thr Ala Met Gly Ala Asn Gly Tyr Lys Thr Ile
465                 470                 475                 480 
Asp Asn Lys Asn Phe Tyr Phe Arg Asn Gly Leu Pro Gln Ile Gly Val
                485                 490                 495     
Phe Lys Gly Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp
            500                 505                 510         
Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg Phe Leu
        515                 520                 525             
His Leu Leu Gly Lys Ile Tyr Tyr Phe Gly Asn Asn Ser Lys Ala Val
    530                 535                 540                 
Thr Gly Trp Gln Thr Ile Asn Gly Lys Val Tyr Tyr Phe Met Pro Asp
545                 550                 555                 560 
Thr Ala Met Ala Ala Ala Gly Gly Leu Phe Glu Ile Asp Gly Val Ile
                565                 570                 575     
Tyr Phe Phe Gly Val Asp Gly Val Lys Ala Pro Gly Ile Tyr Gly Gly
            580                 585                 590         
Gly Phe Val Ser Ile Asn Asp Asn Lys His Tyr Phe Asp Asp Ser Gly
        595                 600                 605             
Val Met Lys Val Gly Tyr Thr Glu Ile Asp Gly Lys His Phe Tyr Phe
    610                 615                 620                 
Ala Glu Asn Gly Glu Met Gln Ile Gly Val Phe Asn Thr Glu Asp Gly
625                 630                 635                 640 
Phe Lys Tyr Phe Ala His His Asn Glu Asp Leu Gly Asn Glu Glu Gly
                645                 650                 655     
Glu Glu Ile Ser Tyr Ser Gly Ile Leu Asn Phe Asn Asn Lys Ile Tyr
            660                 665                 670         
Tyr Phe Asp Asp Ser Phe Thr Ala Val Val Gly Trp Lys Asp Leu Glu
        675                 680                 685             
Asp Gly Ser Lys Tyr Tyr Phe Asp Glu Asp Thr Ala Glu Ala Tyr Ile
    690                 695                 700                 
Gly Leu Ser Leu Ile Asn Asp Gly Gln Tyr Tyr Phe Asn Asp Asp Gly
705                 710                 715                 720 
Ile Met Gln Val Gly Phe Val Thr Ile Asn Asp Lys Val Phe Tyr Phe
                725                 730                 735     
Ser Asp Ser Gly Ile Ile Glu Ser Gly Val Gln Asn Ile Asp Asp Asn
            740                 745                 750         
Tyr Phe Tyr Ile Asp Asp Asn Gly Ile Val Gln Ile Gly Val Phe Asp
        755                 760                 765             
Thr Ser Asp Gly Tyr Lys Tyr Phe Ala Pro Ala Asn Thr Val Asn Asp
    770                 775                 780                 
Asn Ile Tyr Gly Gln Ala Val Glu Tyr Ser Gly Leu Val Arg Val Gly
785                 790                 795                 800 
Glu Asp Val Tyr Tyr Phe Gly Glu Thr Tyr Thr Ile Glu Thr Gly Trp
                805                 810                 815     
Ile Tyr Asp Met Glu Asn Glu Ser Asp Lys Tyr Tyr Phe Asn Pro Glu
            820                 825                 830         
Thr Lys Lys Ala Cys Lys Gly Ile Asn Leu Ile Asp Asp Ile Lys Tyr
        835                 840                 845             
Tyr Phe Asp Glu Lys Gly Ile Met Arg Thr Gly Leu Ile Ser Phe Glu
    850                 855                 860                 
Asn Asn Asn Tyr Tyr Phe Asn Glu Asn Gly Glu Met Gln Phe Gly Tyr
865                 870                 875                 880 
Ile Asn Ile Glu Asp Lys Met Phe Tyr Phe Gly Glu Asp Gly Val Met
                885                 890                 895     
Gln Ile Gly Val Phe Asn Thr Pro Asp Gly Phe Lys Tyr Phe Ala His
            900                 905                 910         
Gln Asn Thr Leu Asp Glu Asn Phe Glu Gly Glu Ser Ile Asn Tyr Thr
        915                 920                 925             
Gly Trp Leu Asp Leu Asp Glu Lys Arg Tyr Tyr Phe Thr Asp Glu Tyr
    930                 935                 940                 
Ile Ala Ala Thr Gly Ser Val Ile Ile Asp Gly Glu Glu Tyr Tyr Phe
945                 950                 955                 960 
Asp Pro Asp Thr Ala Gln Leu Val Ile Ser Glu
                965                 970     


<210> 23
<211> 3339
<212> DNA
<213> Artificial Sequence

<220> 
<223> nucleotide sequence of F54 Gly

<400> 23
atggcaaccg gttggcagac catcgatggc aaaaaatatt attttaatac caacaccgca 60
attgcaagca ccggctatac cattatcaac ggcaaacact tttattttaa caccgacggc 120
attatgcaga ttggtgtgtt taaaggtccg aacggctttg aatactttgc accggcaaat 180
accgatgcca ataatattga aggccaggcc attctgtatc agaatgaatt tctgaccctg 240
aacggcaaaa aatactactt tggcagcgat agcaaagcag ttaccggttg gcgcatcatc 300
aacaataaga aatattactt caacccgaat aatgcaattg cagcaattca tctgtgcacc 360
attaacaacg acaaatatta tttcagctat gacggtattc tgcagaatgg ctacattacc 420
atcgaacgca acaactttta tttcgatgcc aacaacgaaa gcaaaatggt gaccggtgtt 480
ttcaaaggcc ctaatggttt tgagtatttc gctccggcaa acacccataa taacaacatt 540
gaaggtcagg cgatcgttta tcagaacaaa ttcctgacgc tgaatggtaa gaaatactat 600
ttcgataatg acagcaaagc cgtgaccggc tggcagacaa ttgacgggaa gaaatattac 660
tttaatctga ataccgcaga agcagcaacc ggttggcaaa cgatcgacgg taaaaagtac 720
tacttcaacc tgaacacagc cgaagcagcc acaggatggc agactattga tggaaaaaaa 780
tactatttca acaccaacac ctttattgca tctaccggtt ataccagcat taacggtaaa 840
catttctact tcaacaccga tggtatcatg cagatcggcg ttttcaaagg tccaaatggt 900
ttcgaatact ttgcccctgc caatacagat gcaaataaca tcgagggtca ggcaatcctg 960
taccaaaaca aatttctgac cctgaatggg aaaaaatatt actttggtag cgattctaaa 1020
gccgttaccg gtctgcgtac cattgatggt aaaaaatact actttaatac gaatacagcc 1080
gttgcggtta caggctggca gaccattaac gggaaaaaat actattttaa cacaaatacc 1140
agcattgcct caacgggtta taccattatt tcgggtaaac acttctactt taataccgat 1200
ggtattatgc aaatcggagt ctttaaagga cctgatgggt tcgaatattt tgcgcctgcg 1260
aacactgatg cgaacaatat cgaaggacag gcaatccgct atcagaatcg ctttctgtat 1320
ctgcacgaca acatctatta ttttggcaac aattcaaaag cagccaccgg ctgggttaca 1380
attgatggca accgctacta tttcgaaccg aataccgcaa tgggtgcaaa tggctacaaa 1440
accatcgata ataaaaattt ctattttcgc aacggtctgc cgcagatcgg ggtatttaaa 1500
ggtagcaacg gcttcgaata cttcgctcca gcgaatacgg acgcgaacaa tattgagggt 1560
caagcgattc gttatcaaaa ccgttttctg catctgctgg gcaaaatcta ctactttggc 1620
aataacagta aagcagttac tggatggcag acaatcaatg gtaaagtgta ctattttatg 1680
ccggataccg ccatggcagc agccggtggt ctgtttgaaa ttgatggcgt gatctatttt 1740
tttggtgtgg atggtgttaa agcaccggga atatacggtg gtaccggctt tgtgaccgtg 1800
ggtgatgata aatactattt caatccgatt aacggtggtg cagcgagcat tggcgaaacc 1860
atcatcgatg acaaaaacta ttatttcaac cagagcggtg tgctgcagac cggtgtgttt 1920
agcaccgaag atggctttaa atattttgcg ccagcgaaca ccctggatga aaacctggaa 1980
ggcgaagcga ttgattttac cggcaaactg atcatcgatg aaaacatcta ttacttcgat 2040
gataactatc gtggtgcggt ggaatggaaa gaactggatg gcgaaatgca ttatttttct 2100
ccggaaaccg gtaaagcgtt taaaggcctg aaccagatcg gcgattacaa atactacttc 2160
aacagcgatg gcgtgatgca gaaaggcttt gtgagcatca acgataacaa acactatttc 2220
gatgatagcg gtgtgatgaa agtgggctat accgaaattg atggcaaaca tttctacttc 2280
gcggaaaacg gcgaaatgca gattggcgtg ttcaataccg aagatggttt caaatacttc 2340
gcgcaccata acgaagatct gggtaacgaa gaaggcgaag aaattagcta tagcggcatc 2400
ctgaacttca acaacaaaat ctactacttt gatgatagct ttaccgcggt ggtgggctgg 2460
aaagatctgg aagatggcag caaatattat ttcgatgaag ataccgcgga agcgtatatt 2520
ggcctgagcc tgattaacga tggccagtac tattttaacg atgatggcat tatgcaggtg 2580
ggtttcgtga ccattaatga taaagtgttc tatttcagcg atagcggcat tattgaaagc 2640
ggcgtgcaga acattgatga taactacttc tacatcgatg ataacggcat tgtgcagatc 2700
ggcgtttttg ataccagcga tggctacaaa tatttcgcac cggccaatac cgtgaacgat 2760
aacatttatg gccaggcggt ggaatatagc ggtctggtgc gtgtgggcga agatgtgtat 2820
tatttcggcg aaacctatac catcgaaacc ggctggattt atgatatgga aaacgaaagc 2880
gataaatatt actttaatcc ggaaacgaaa aaagcgtgca aaggcattaa cctgatcgat 2940
gatatcaaat actattttga tgaaaaaggc attatgcgta ccggtctgat tagcttcgaa 3000
aacaacaact attacttcaa cgaaaacggt gaaatgcagt tcggctacat caacatcgaa 3060
gataaaatgt tctacttcgg cgaagatggt gttatgcaga ttggtgtttt taacaccccg 3120
gatggcttca aatactttgc ccatcagaat accctggatg aaaatttcga aggtgaaagc 3180
attaactata ccggctggct ggatctggat gaaaaacgct actacttcac cgatgaatac 3240
attgcggcga ccggcagcgt gattattgat ggcgaagaat actacttcga tccggatacc 3300
gcgcagctgg tgattagcga acatcatcat catcaccat                        3339

<210> 24
<211> 1113
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid of F54Gly

<400> 24
Met Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn
 1               5                  10                  15      
Thr Asn Thr Ala Ile Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys
            20                  25                  30          
His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys
        35                  40                  45              
Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn
    50                  55                  60                  
Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu
65                  70                  75                  80  
Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly
                85                  90                  95      
Trp Arg Ile Ile Asn Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala
            100                 105                 110         
Ile Ala Ala Ile His Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe
        115                 120                 125             
Ser Tyr Asp Gly Ile Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn
    130                 135                 140                 
Asn Phe Tyr Phe Asp Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val
145                 150                 155                 160 
Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His
                165                 170                 175     
Asn Asn Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu
            180                 185                 190         
Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val
        195                 200                 205             
Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn
    210                 215                 220                 
Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr
225                 230                 235                 240 
Tyr Phe Asn Leu Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile
                245                 250                 255     
Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr
            260                 265                 270         
Gly Tyr Thr Ser Ile Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly
        275                 280                 285             
Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe
    290                 295                 300                 
Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Leu
305                 310                 315                 320 
Tyr Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly
                325                 330                 335     
Ser Asp Ser Lys Ala Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys
            340                 345                 350         
Tyr Tyr Phe Asn Thr Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr
        355                 360                 365             
Ile Asn Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser
    370                 375                 380                 
Thr Gly Tyr Thr Ile Ile Ser Gly Lys His Phe Tyr Phe Asn Thr Asp
385                 390                 395                 400 
Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr
                405                 410                 415     
Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile
            420                 425                 430         
Arg Tyr Gln Asn Arg Phe Leu Tyr Leu His Asp Asn Ile Tyr Tyr Phe
        435                 440                 445             
Gly Asn Asn Ser Lys Ala Ala Thr Gly Trp Val Thr Ile Asp Gly Asn
    450                 455                 460                 
Arg Tyr Tyr Phe Glu Pro Asn Thr Ala Met Gly Ala Asn Gly Tyr Lys
465                 470                 475                 480 
Thr Ile Asp Asn Lys Asn Phe Tyr Phe Arg Asn Gly Leu Pro Gln Ile
                485                 490                 495     
Gly Val Phe Lys Gly Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn
            500                 505                 510         
Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg
        515                 520                 525             
Phe Leu His Leu Leu Gly Lys Ile Tyr Tyr Phe Gly Asn Asn Ser Lys
    530                 535                 540                 
Ala Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Val Tyr Tyr Phe Met
545                 550                 555                 560 
Pro Asp Thr Ala Met Ala Ala Ala Gly Gly Leu Phe Glu Ile Asp Gly
                565                 570                 575     
Val Ile Tyr Phe Phe Gly Val Asp Gly Val Lys Ala Pro Gly Ile Tyr
            580                 585                 590         
Gly Gly Thr Gly Phe Val Thr Val Gly Asp Asp Lys Tyr Tyr Phe Asn
        595                 600                 605             
Pro Ile Asn Gly Gly Ala Ala Ser Ile Gly Glu Thr Ile Ile Asp Asp
    610                 615                 620                 
Lys Asn Tyr Tyr Phe Asn Gln Ser Gly Val Leu Gln Thr Gly Val Phe
625                 630                 635                 640 
Ser Thr Glu Asp Gly Phe Lys Tyr Phe Ala Pro Ala Asn Thr Leu Asp
                645                 650                 655     
Glu Asn Leu Glu Gly Glu Ala Ile Asp Phe Thr Gly Lys Leu Ile Ile
            660                 665                 670         
Asp Glu Asn Ile Tyr Tyr Phe Asp Asp Asn Tyr Arg Gly Ala Val Glu
        675                 680                 685             
Trp Lys Glu Leu Asp Gly Glu Met His Tyr Phe Ser Pro Glu Thr Gly
    690                 695                 700                 
Lys Ala Phe Lys Gly Leu Asn Gln Ile Gly Asp Tyr Lys Tyr Tyr Phe
705                 710                 715                 720 
Asn Ser Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile Asn Asp Asn
                725                 730                 735     
Lys His Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly Tyr Thr Glu
            740                 745                 750         
Ile Asp Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile
        755                 760                 765             
Gly Val Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala His His Asn
    770                 775                 780                 
Glu Asp Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile
785                 790                 795                 800 
Leu Asn Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala
                805                 810                 815     
Val Val Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp
            820                 825                 830         
Glu Asp Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly
        835                 840                 845             
Gln Tyr Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly Phe Val Thr
    850                 855                 860                 
Ile Asn Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser
865                 870                 875                 880 
Gly Val Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly
                885                 890                 895     
Ile Val Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe
            900                 905                 910         
Ala Pro Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu
        915                 920                 925             
Tyr Ser Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu
    930                 935                 940                 
Thr Tyr Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser
945                 950                 955                 960 
Asp Lys Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile
                965                 970                 975     
Asn Leu Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met
            980                 985                 990         
Arg Thr Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu
        995                 1000                1005            
Asn Gly Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe
    1010                1015                1020                
Tyr Phe Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe Asn Thr Pro
1025                1030                1035                1040
Asp Gly Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe
                1045                1050                1055    
Glu Gly Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys
            1060                1065                1070        
Arg Tyr Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile
        1075                1080                1085            
Ile Asp Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val
    1090                1095                1100                
Ile Ser Glu His His His His His His
1105                1110            


<210> 25
<211> 3324
<212> DNA
<213> Artificial Sequence

<220> 
<223> nucleotide sequence of F54 New

<400> 25
atggcaaccg gttggcagac catcgatggc aaaaaatatt attttaatac caacaccgca 60
attgcaagca ccggctatac cattatcaac ggcaaacact tttattttaa caccgacggc 120
attatgcaga ttggtgtgtt taaaggtccg aacggctttg aatactttgc accggcaaat 180
accgatgcca ataatattga aggccaggcc attctgtatc agaatgaatt tctgaccctg 240
aacggcaaaa aatactactt tggcagcgat agcaaagcag ttaccggttg gcgcatcatc 300
aacaataaga aatattactt caacccgaat aatgcaattg cagcaattca tctgtgcacc 360
attaacaacg acaaatatta tttcagctat gacggtattc tgcagaatgg ctacattacc 420
atcgaacgca acaactttta tttcgatgcc aacaacgaaa gcaaaatggt gaccggtgtt 480
ttcaaaggcc ctaatggttt tgagtatttc gctccggcaa acacccataa taacaacatt 540
gaaggtcagg cgatcgttta tcagaacaaa ttcctgacgc tgaatggtaa gaaatactat 600
ttcgataatg acagcaaagc cgtgaccggc tggcagacaa ttgacgggaa gaaatattac 660
tttaatctga ataccgcaga agcagcaacc ggttggcaaa cgatcgacgg taaaaagtac 720
tacttcaacc tgaacacagc cgaagcagcc acaggatggc agactattga tggaaaaaaa 780
tactatttca acaccaacac ctttattgca tctaccggtt ataccagcat taacggtaaa 840
catttctact tcaacaccga tggtatcatg cagatcggcg ttttcaaagg tccaaatggt 900
ttcgaatact ttgcccctgc caatacagat gcaaataaca tcgagggtca ggcaatcctg 960
taccaaaaca aatttctgac cctgaatggg aaaaaatatt actttggtag cgattctaaa 1020
gccgttaccg gtctgcgtac cattgatggt aaaaaatact actttaatac gaatacagcc 1080
gttgcggtta caggctggca gaccattaac gggaaaaaat actattttaa cacaaatacc 1140
agcattgcct caacgggtta taccattatt tcgggtaaac acttctactt taataccgat 1200
ggtattatgc aaatcggagt ctttaaagga cctgatgggt tcgaatattt tgcgcctgcg 1260
aacactgatg cgaacaatat cgaaggacag gcaatccgct atcagaatcg ctttctgtat 1320
ctgcacgaca acatctatta ttttggcaac aattcaaaag cagccaccgg ctgggttaca 1380
attgatggca accgctacta tttcgaaccg aataccgcaa tgggtgcaaa tggctacaaa 1440
accatcgata ataaaaattt ctattttcgc aacggtctgc cgcagatcgg ggtatttaaa 1500
ggtagcaacg gcttcgaata cttcgctcca gcgaatacgg acgcgaacaa tattgagggt 1560
caagcgattc gttatcaaaa ccgttttctg catctgctgg gcaaaatcta ctactttggc 1620
aataacagta aagcagttac tggatggcag acaatcaatg gtaaagtgta ctattttatg 1680
ccggataccg ccatggcagc agccggtggt ctgtttgaaa ttgatggcgt gatctatttt 1740
tttggtgtgg atggtgttaa agcagttacc ggctttgtga ccgtgggtga tgataaatac 1800
tatttcaatc cgattaacgg tggtgcagcg agcattggcg aaaccatcat cgatgacaaa 1860
aactattatt tcaaccagag cggtgtgctg cagaccggtg tgtttagcac cgaagatggc 1920
tttaaatatt ttgcgccagc gaacaccctg gatgaaaacc tggaaggcga agcgattgat 1980
tttaccggca aactgatcat cgatgaaaac atctattact tcgatgataa ctatcgtggt 2040
gcggtggaat ggaaagaact ggatggcgaa atgcattatt tttctccgga aaccggtaaa 2100
gcgtttaaag gcctgaacca gatcggcgat tacaaatact acttcaacag cgatggcgtg 2160
atgcagaaag gctttgtgag catcaacgat aacaaacact atttcgatga tagcggtgtg 2220
atgaaagtgg gctataccga aattgatggc aaacatttct acttcgcgga aaacggcgaa 2280
atgcagattg gcgtgttcaa taccgaagat ggtttcaaat acttcgcgca ccataacgaa 2340
gatctgggta acgaagaagg cgaagaaatt agctatagcg gcatcctgaa cttcaacaac 2400
aaaatctact actttgatga tagctttacc gcggtggtgg gctggaaaga tctggaagat 2460
ggcagcaaat attatttcga tgaagatacc gcggaagcgt atattggcct gagcctgatt 2520
aacgatggcc agtactattt taacgatgat ggcattatgc aggtgggttt cgtgaccatt 2580
aatgataaag tgttctattt cagcgatagc ggcattattg aaagcggcgt gcagaacatt 2640
gatgataact acttctacat cgatgataac ggcattgtgc agatcggcgt ttttgatacc 2700
agcgatggct acaaatattt cgcaccggcc aataccgtga acgataacat ttatggccag 2760
gcggtggaat atagcggtct ggtgcgtgtg ggcgaagatg tgtattattt cggcgaaacc 2820
tataccatcg aaaccggctg gatttatgat atggaaaacg aaagcgataa atattacttt 2880
aatccggaaa cgaaaaaagc gtgcaaaggc attaacctga tcgatgatat caaatactat 2940
tttgatgaaa aaggcattat gcgtaccggt ctgattagct tcgaaaacaa caactattac 3000
ttcaacgaaa acggtgaaat gcagttcggc tacatcaaca tcgaagataa aatgttctac 3060
ttcggcgaag atggtgttat gcagattggt gtttttaaca ccccggatgg cttcaaatac 3120
tttgcccatc agaataccct ggatgaaaat ttcgaaggtg aaagcattaa ctataccggc 3180
tggctggatc tggatgaaaa acgctactac ttcaccgatg aatacattgc ggcgaccggc 3240
agcgtgatta ttgatggcga agaatactac ttcgatccgg ataccgcgca gctggtgatt 3300
agcgaacatc atcatcatca ccat                                        3324

<210> 26
<211> 1108
<212> PRT
<213> Artificial Sequence

<220> 
<223> 26 F54 New

<400> 26
Met Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn
 1               5                  10                  15      
Thr Asn Thr Ala Ile Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys
            20                  25                  30          
His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys
        35                  40                  45              
Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn
    50                  55                  60                  
Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu
65                  70                  75                  80  
Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly
                85                  90                  95      
Trp Arg Ile Ile Asn Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala
            100                 105                 110         
Ile Ala Ala Ile His Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe
        115                 120                 125             
Ser Tyr Asp Gly Ile Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn
    130                 135                 140                 
Asn Phe Tyr Phe Asp Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val
145                 150                 155                 160 
Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His
                165                 170                 175     
Asn Asn Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu
            180                 185                 190         
Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val
        195                 200                 205             
Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn
    210                 215                 220                 
Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr
225                 230                 235                 240 
Tyr Phe Asn Leu Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile
                245                 250                 255     
Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr
            260                 265                 270         
Gly Tyr Thr Ser Ile Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly
        275                 280                 285             
Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe
    290                 295                 300                 
Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Leu
305                 310                 315                 320 
Tyr Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly
                325                 330                 335     
Ser Asp Ser Lys Ala Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys
            340                 345                 350         
Tyr Tyr Phe Asn Thr Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr
        355                 360                 365             
Ile Asn Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser
    370                 375                 380                 
Thr Gly Tyr Thr Ile Ile Ser Gly Lys His Phe Tyr Phe Asn Thr Asp
385                 390                 395                 400 
Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr
                405                 410                 415     
Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile
            420                 425                 430         
Arg Tyr Gln Asn Arg Phe Leu Tyr Leu His Asp Asn Ile Tyr Tyr Phe
        435                 440                 445             
Gly Asn Asn Ser Lys Ala Ala Thr Gly Trp Val Thr Ile Asp Gly Asn
    450                 455                 460                 
Arg Tyr Tyr Phe Glu Pro Asn Thr Ala Met Gly Ala Asn Gly Tyr Lys
465                 470                 475                 480 
Thr Ile Asp Asn Lys Asn Phe Tyr Phe Arg Asn Gly Leu Pro Gln Ile
                485                 490                 495     
Gly Val Phe Lys Gly Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn
            500                 505                 510         
Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg
        515                 520                 525             
Phe Leu His Leu Leu Gly Lys Ile Tyr Tyr Phe Gly Asn Asn Ser Lys
    530                 535                 540                 
Ala Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Val Tyr Tyr Phe Met
545                 550                 555                 560 
Pro Asp Thr Ala Met Ala Ala Ala Gly Gly Leu Phe Glu Ile Asp Gly
                565                 570                 575     
Val Ile Tyr Phe Phe Gly Val Asp Gly Val Lys Ala Val Thr Gly Phe
            580                 585                 590         
Val Thr Val Gly Asp Asp Lys Tyr Tyr Phe Asn Pro Ile Asn Gly Gly
        595                 600                 605             
Ala Ala Ser Ile Gly Glu Thr Ile Ile Asp Asp Lys Asn Tyr Tyr Phe
    610                 615                 620                 
Asn Gln Ser Gly Val Leu Gln Thr Gly Val Phe Ser Thr Glu Asp Gly
625                 630                 635                 640 
Phe Lys Tyr Phe Ala Pro Ala Asn Thr Leu Asp Glu Asn Leu Glu Gly
                645                 650                 655     
Glu Ala Ile Asp Phe Thr Gly Lys Leu Ile Ile Asp Glu Asn Ile Tyr
            660                 665                 670         
Tyr Phe Asp Asp Asn Tyr Arg Gly Ala Val Glu Trp Lys Glu Leu Asp
        675                 680                 685             
Gly Glu Met His Tyr Phe Ser Pro Glu Thr Gly Lys Ala Phe Lys Gly
    690                 695                 700                 
Leu Asn Gln Ile Gly Asp Tyr Lys Tyr Tyr Phe Asn Ser Asp Gly Val
705                 710                 715                 720 
Met Gln Lys Gly Phe Val Ser Ile Asn Asp Asn Lys His Tyr Phe Asp
                725                 730                 735     
Asp Ser Gly Val Met Lys Val Gly Tyr Thr Glu Ile Asp Gly Lys His
            740                 745                 750         
Phe Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile Gly Val Phe Asn Thr
        755                 760                 765             
Glu Asp Gly Phe Lys Tyr Phe Ala His His Asn Glu Asp Leu Gly Asn
    770                 775                 780                 
Glu Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile Leu Asn Phe Asn Asn
785                 790                 795                 800 
Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala Val Val Gly Trp Lys
                805                 810                 815     
Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp Glu Asp Thr Ala Glu
            820                 825                 830         
Ala Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly Gln Tyr Tyr Phe Asn
        835                 840                 845             
Asp Asp Gly Ile Met Gln Val Gly Phe Val Thr Ile Asn Asp Lys Val
    850                 855                 860                 
Phe Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser Gly Val Gln Asn Ile
865                 870                 875                 880 
Asp Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly Ile Val Gln Ile Gly
                885                 890                 895     
Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe Ala Pro Ala Asn Thr
            900                 905                 910         
Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu Tyr Ser Gly Leu Val
        915                 920                 925             
Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu Thr Tyr Thr Ile Glu
    930                 935                 940                 
Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser Asp Lys Tyr Tyr Phe
945                 950                 955                 960 
Asn Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile Asn Leu Ile Asp Asp
                965                 970                 975     
Ile Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met Arg Thr Gly Leu Ile
            980                 985                 990         
Ser Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu Asn Gly Glu Met Gln
        995                 1000                1005            
Phe Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe Tyr Phe Gly Glu Asp
    1010                1015                1020                
Gly Val Met Gln Ile Gly Val Phe Asn Thr Pro Asp Gly Phe Lys Tyr
1025                1030                1035                1040
Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe Glu Gly Glu Ser Ile
                1045                1050                1055    
Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys Arg Tyr Tyr Phe Thr
            1060                1065                1070        
Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile Ile Asp Gly Glu Glu
        1075                1080                1085            
Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val Ile Ser Glu His His
    1090                1095                1100                
His His His His
1105            


<210> 27
<211> 3387
<212> DNA
<213> Artificial Sequence

<220> 
<223> nucleotide sequence of F5 ToxB

<400> 27
atggcaaccg gttggcagac catcgatggc aaaaaatatt attttaatac caacaccgca 60
attgcaagca ccggctatac cattatcaac ggcaaacact tttattttaa caccgacggc 120
attatgcaga ttggtgtgtt taaaggtccg aacggctttg aatactttgc accggcaaat 180
accgatgcca ataatattga aggccaggcc attctgtatc agaatgaatt tctgaccctg 240
aacggcaaaa aatactactt tggcagcgat agcaaagcag ttaccggttg gcgcatcatc 300
aacaataaga aatattactt caacccgaat aatgcaattg cagcaattca tctgtgcacc 360
attaacaacg acaaatatta tttcagctat gacggtattc tgcagaatgg ctacattacc 420
atcgaacgca acaactttta tttcgatgcc aacaacgaaa gcaaaatggt gaccggtgtt 480
ttcaaaggcc ctaatggttt tgagtatttc gctccggcaa acacccataa taacaacatt 540
gaaggtcagg cgatcgttta tcagaacaaa ttcctgacgc tgaatggtaa gaaatactat 600
ttcgataatg acagcaaagc cgtgaccggc tggcagacaa ttgacgggaa gaaatattac 660
tttaatctga ataccgcaga agcagcaacc ggttggcaaa cgatcgacgg taaaaagtac 720
tacttcaacc tgaacacagc cgaagcagcc acaggatggc agactattga tggaaaaaaa 780
tactatttca acaccaacac ctttattgca tctaccggtt ataccagcat taacggtaaa 840
catttctact tcaacaccga tggtatcatg cagatcggcg ttttcaaagg tccaaatggt 900
ttcgaatact ttgcccctgc caatacagat gcaaataaca tcgagggtca ggcaatcctg 960
taccaaaaca aatttctgac cctgaatggg aaaaaatatt actttggtag cgattctaaa 1020
gccgttaccg gtctgcgtac cattgatggt aaaaaatact actttaatac gaatacagcc 1080
gttgcggtta caggctggca gaccattaac gggaaaaaat actattttaa cacaaatacc 1140
agcattgcct caacgggtta taccattatt tcgggtaaac acttctactt taataccgat 1200
ggtattatgc aaatcggagt ctttaaagga cctgatgggt tcgaatattt tgcgcctgcg 1260
aacactgatg cgaacaatat cgaaggacag gcaatccgct atcagaatcg ctttctgtat 1320
ctgcacgaca acatctatta ttttggcaac aattcaaaag cagccaccgg ctgggttaca 1380
attgatggca accgctacta tttcgaaccg aataccgcaa tgggtgcaaa tggctacaaa 1440
accatcgata ataaaaattt ctattttcgc aacggtctgc cgcagatcgg ggtatttaaa 1500
ggtagcaacg gcttcgaata cttcgctcca gcgaatacgg acgcgaacaa tattgagggt 1560
caagcgattc gttatcaaaa ccgttttctg catctgctgg gcaaaatcta ctactttggc 1620
aataacagta aagcagttac tggatggcag acaatcaatg gtaaagtgta ctattttatg 1680
ccggataccg ccatggcagc agccggtggt ctgtttgaaa ttgatggcgt gatctatttt 1740
tttggtgtgg atggtgttaa agcagtgagc ggtctgattt atattaacga tagcctgtat 1800
tactttaaac caccggtgaa taacctgatt accggctttg tgaccgtggg tgatgataaa 1860
tactatttca atccgattaa cggtggtgca gcgagcattg gcgaaaccat catcgatgac 1920
aaaaactatt atttcaacca gagcggtgtg ctgcagaccg gtgtgtttag caccgaagat 1980
ggctttaaat attttgcgcc agcgaacacc ctggatgaaa acctggaagg cgaagcgatt 2040
gattttaccg gcaaactgat catcgatgaa aacatctatt acttcgatga taactatcgt 2100
ggtgcggtgg aatggaaaga actggatggc gaaatgcatt atttttctcc ggaaaccggt 2160
aaagcgttta aaggcctgaa ccagatcggc gattacaaat actacttcaa cagcgatggc 2220
gtgatgcaga aaggctttgt gagcatcaac gataacaaac actatttcga tgatagcggt 2280
gtgatgaaag tgggctatac cgaaattgat ggcaaacatt tctacttcgc ggaaaacggc 2340
gaaatgcaga ttggcgtgtt caataccgaa gatggtttca aatacttcgc gcaccataac 2400
gaagatctgg gtaacgaaga aggcgaagaa attagctata gcggcatcct gaacttcaac 2460
aacaaaatct actactttga tgatagcttt accgcggtgg tgggctggaa agatctggaa 2520
gatggcagca aatattattt cgatgaagat accgcggaag cgtatattgg cctgagcctg 2580
attaacgatg gccagtacta ttttaacgat gatggcatta tgcaggtggg tttcgtgacc 2640
attaatgata aagtgttcta tttcagcgat agcggcatta ttgaaagcgg cgtgcagaac 2700
attgatgata actacttcta catcgatgat aacggcattg tgcagatcgg cgtttttgat 2760
accagcgatg gctacaaata tttcgcaccg gccaataccg tgaacgataa catttatggc 2820
caggcggtgg aatatagcgg tctggtgcgt gtgggcgaag atgtgtatta tttcggcgaa 2880
acctatacca tcgaaaccgg ctggatttat gatatggaaa acgaaagcga taaatattac 2940
tttaatccgg aaacgaaaaa agcgtgcaaa ggcattaacc tgatcgatga tatcaaatac 3000
tattttgatg aaaaaggcat tatgcgtacc ggtctgatta gcttcgaaaa caacaactat 3060
tacttcaacg aaaacggtga aatgcagttc ggctacatca acatcgaaga taaaatgttc 3120
tacttcggcg aagatggtgt tatgcagatt ggtgttttta acaccccgga tggcttcaaa 3180
tactttgccc atcagaatac cctggatgaa aatttcgaag gtgaaagcat taactatacc 3240
ggctggctgg atctggatga aaaacgctac tacttcaccg atgaatacat tgcggcgacc 3300
ggcagcgtga ttattgatgg cgaagaatac tacttcgatc cggataccgc gcagctggtg 3360
attagcgaac atcatcatca tcaccat                                     3387

<210> 28
<211> 1129
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of F5 ToxB

<400> 28
Met Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn
 1               5                  10                  15      
Thr Asn Thr Ala Ile Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys
            20                  25                  30          
His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys
        35                  40                  45              
Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn
    50                  55                  60                  
Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu
65                  70                  75                  80  
Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly
                85                  90                  95      
Trp Arg Ile Ile Asn Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala
            100                 105                 110         
Ile Ala Ala Ile His Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe
        115                 120                 125             
Ser Tyr Asp Gly Ile Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn
    130                 135                 140                 
Asn Phe Tyr Phe Asp Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val
145                 150                 155                 160 
Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His
                165                 170                 175     
Asn Asn Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu
            180                 185                 190         
Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val
        195                 200                 205             
Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn
    210                 215                 220                 
Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr
225                 230                 235                 240 
Tyr Phe Asn Leu Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile
                245                 250                 255     
Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr
            260                 265                 270         
Gly Tyr Thr Ser Ile Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly
        275                 280                 285             
Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe
    290                 295                 300                 
Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Leu
305                 310                 315                 320 
Tyr Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly
                325                 330                 335     
Ser Asp Ser Lys Ala Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys
            340                 345                 350         
Tyr Tyr Phe Asn Thr Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr
        355                 360                 365             
Ile Asn Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser
    370                 375                 380                 
Thr Gly Tyr Thr Ile Ile Ser Gly Lys His Phe Tyr Phe Asn Thr Asp
385                 390                 395                 400 
Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr
                405                 410                 415     
Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile
            420                 425                 430         
Arg Tyr Gln Asn Arg Phe Leu Tyr Leu His Asp Asn Ile Tyr Tyr Phe
        435                 440                 445             
Gly Asn Asn Ser Lys Ala Ala Thr Gly Trp Val Thr Ile Asp Gly Asn
    450                 455                 460                 
Arg Tyr Tyr Phe Glu Pro Asn Thr Ala Met Gly Ala Asn Gly Tyr Lys
465                 470                 475                 480 
Thr Ile Asp Asn Lys Asn Phe Tyr Phe Arg Asn Gly Leu Pro Gln Ile
                485                 490                 495     
Gly Val Phe Lys Gly Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn
            500                 505                 510         
Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg
        515                 520                 525             
Phe Leu His Leu Leu Gly Lys Ile Tyr Tyr Phe Gly Asn Asn Ser Lys
    530                 535                 540                 
Ala Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Val Tyr Tyr Phe Met
545                 550                 555                 560 
Pro Asp Thr Ala Met Ala Ala Ala Gly Gly Leu Phe Glu Ile Asp Gly
                565                 570                 575     
Val Ile Tyr Phe Phe Gly Val Asp Gly Val Lys Ala Val Ser Gly Leu
            580                 585                 590         
Ile Tyr Ile Asn Asp Ser Leu Tyr Tyr Phe Lys Pro Pro Val Asn Asn
        595                 600                 605             
Leu Ile Thr Gly Phe Val Thr Val Gly Asp Asp Lys Tyr Tyr Phe Asn
    610                 615                 620                 
Pro Ile Asn Gly Gly Ala Ala Ser Ile Gly Glu Thr Ile Ile Asp Asp
625                 630                 635                 640 
Lys Asn Tyr Tyr Phe Asn Gln Ser Gly Val Leu Gln Thr Gly Val Phe
                645                 650                 655     
Ser Thr Glu Asp Gly Phe Lys Tyr Phe Ala Pro Ala Asn Thr Leu Asp
            660                 665                 670         
Glu Asn Leu Glu Gly Glu Ala Ile Asp Phe Thr Gly Lys Leu Ile Ile
        675                 680                 685             
Asp Glu Asn Ile Tyr Tyr Phe Asp Asp Asn Tyr Arg Gly Ala Val Glu
    690                 695                 700                 
Trp Lys Glu Leu Asp Gly Glu Met His Tyr Phe Ser Pro Glu Thr Gly
705                 710                 715                 720 
Lys Ala Phe Lys Gly Leu Asn Gln Ile Gly Asp Tyr Lys Tyr Tyr Phe
                725                 730                 735     
Asn Ser Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile Asn Asp Asn
            740                 745                 750         
Lys His Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly Tyr Thr Glu
        755                 760                 765             
Ile Asp Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile
    770                 775                 780                 
Gly Val Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala His His Asn
785                 790                 795                 800 
Glu Asp Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile
                805                 810                 815     
Leu Asn Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala
            820                 825                 830         
Val Val Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp
        835                 840                 845             
Glu Asp Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly
    850                 855                 860                 
Gln Tyr Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly Phe Val Thr
865                 870                 875                 880 
Ile Asn Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser
                885                 890                 895     
Gly Val Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly
            900                 905                 910         
Ile Val Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe
        915                 920                 925             
Ala Pro Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu
    930                 935                 940                 
Tyr Ser Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu
945                 950                 955                 960 
Thr Tyr Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser
                965                 970                 975     
Asp Lys Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile
            980                 985                 990         
Asn Leu Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met
        995                 1000                1005            
Arg Thr Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu
    1010                1015                1020                
Asn Gly Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe
1025                1030                1035                1040
Tyr Phe Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe Asn Thr Pro
                1045                1050                1055    
Asp Gly Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe
            1060                1065                1070        
Glu Gly Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys
        1075                1080                1085            
Arg Tyr Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile
    1090                1095                1100                
Ile Asp Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val
1105                1110                1115                1120
Ile Ser Glu His His His His His His
                1125                


<210> 29
<211> 2985
<212> DNA
<213> Artificial Sequence

<220> 
<223> nucleotide sequence of F52 new

<400> 29
atggcaaccg gttggcagac catcgatggc aaaaaatatt attttaatac caacaccgca 60
attgcaagca ccggctatac cattatcaac ggcaaacact tttattttaa caccgacggc 120
attatgcaga ttggtgtgtt taaaggtccg aacggctttg aatactttgc accggcaaat 180
accgatgcca ataatattga aggccaggcc attctgtatc agaatgaatt tctgaccctg 240
aacggcaaaa aatactactt tggcagcgat agcaaagcag ttaccggttg gcgcatcatc 300
aacaataaga aatattactt caacccgaat aatgcaattg cagcaattca tctgtgcacc 360
attaacaacg acaaatatta tttcagctat gacggtattc tgcagaatgg ctacattacc 420
atcgaacgca acaactttta tttcgatgcc aacaacgaaa gcaaaatggt gaccggtgtt 480
ttcaaaggcc ctaatggttt tgagtatttc gctccggcaa acacccataa taacaacatt 540
gaaggtcagg cgatcgttta tcagaacaaa ttcctgacgc tgaatggtaa gaaatactat 600
ttcgataatg acagcaaagc cgtgaccggc tggcagacaa ttgacgggaa gaaatattac 660
tttaatctga ataccgcaga agcagcaacc ggttggcaaa cgatcgacgg taaaaagtac 720
tacttcaacc tgaacacagc cgaagcagcc acaggatggc agactattga tggaaaaaaa 780
tactatttca acaccaacac ctttattgca tctaccggtt ataccagcat taacggtaaa 840
catttctact tcaacaccga tggtatcatg cagatcggcg ttttcaaagg tccaaatggt 900
ttcgaatact ttgcccctgc caatacagat gcaaataaca tcgagggtca ggcaatcctg 960
taccaaaaca aatttctgac cctgaatggg aaaaaatatt actttggtag cgattctaaa 1020
gccgttaccg gtctgcgtac cattgatggt aaaaaatact actttaatac gaatacagcc 1080
gttgcggtta caggctggca gaccattaac gggaaaaaat actattttaa cacaaatacc 1140
agcattgcct caacgggtta taccattatt tcgggtaaac acttctactt taataccgat 1200
ggtattatgc aaatcggagt ctttaaagga cctgatgggt tcgaatattt tgcgcctgcg 1260
aacactgatg cgaacaatat cgaaggacag gcaatccgct atcagaatcg ctttctgtat 1320
ctgcacgaca acatctatta ttttggcaac aattcaaaag cagccaccgg ctgggttaca 1380
attgatggca accgctacta tttcgaaccg aataccgcaa tgggtgcaaa tggctacaaa 1440
accatcgata ataaaaattt ctattttcgc aacggtctgc cgcagatcgg ggtatttaaa 1500
ggtagcaacg gcttcgaata cttcgctcca gcgaatacgg acgcgaacaa tattgagggt 1560
caagcgattc gttatcaaaa ccgttttctg catctgctgg gcaaaatcta ctactttggc 1620
aataacagta aagcagttac tggatggcag acaatcaatg gtaaagtgta ctattttatg 1680
ccggataccg ccatggcagc agccggtggt ctgtttgaaa ttgatggcgt gatctatttt 1740
tttggtgtgg atggtgttaa agcagtgaaa ggcctgaacc agatcggcga ttacaaatac 1800
tacttcaaca gcgatggcgt gatgcagaaa ggctttgtga gcatcaacga taacaaacac 1860
tatttcgatg atagcggtgt gatgaaagtg ggctataccg aaattgatgg caaacatttc 1920
tacttcgcgg aaaacggcga aatgcagatt ggcgtgttca ataccgaaga tggtttcaaa 1980
tacttcgcgc accataacga agatctgggt aacgaagaag gcgaagaaat tagctatagc 2040
ggcatcctga acttcaacaa caaaatctac tactttgatg atagctttac cgcggtggtg 2100
ggctggaaag atctggaaga tggcagcaaa tattatttcg atgaagatac cgcggaagcg 2160
tatattggcc tgagcctgat taacgatggc cagtactatt ttaacgatga tggcattatg 2220
caggtgggtt tcgtgaccat taatgataaa gtgttctatt tcagcgatag cggcattatt 2280
gaaagcggcg tgcagaacat tgatgataac tacttctaca tcgatgataa cggcattgtg 2340
cagatcggcg tttttgatac cagcgatggc tacaaatatt tcgcaccggc caataccgtg 2400
aacgataaca tttatggcca ggcggtggaa tatagcggtc tggtgcgtgt gggcgaagat 2460
gtgtattatt tcggcgaaac ctataccatc gaaaccggct ggatttatga tatggaaaac 2520
gaaagcgata aatattactt taatccggaa acgaaaaaag cgtgcaaagg cattaacctg 2580
atcgatgata tcaaatacta ttttgatgaa aaaggcatta tgcgtaccgg tctgattagc 2640
ttcgaaaaca acaactatta cttcaacgaa aacggtgaaa tgcagttcgg ctacatcaac 2700
atcgaagata aaatgttcta cttcggcgaa gatggtgtta tgcagattgg tgtttttaac 2760
accccggatg gcttcaaata ctttgcccat cagaataccc tggatgaaaa tttcgaaggt 2820
gaaagcatta actataccgg ctggctggat ctggatgaaa aacgctacta cttcaccgat 2880
gaatacattg cggcgaccgg cagcgtgatt attgatggcg aagaatacta cttcgatccg 2940
gataccgcgc agctggtgat tagcgaacat catcatcatc accat                 2985

<210> 30
<211> 995
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of F52 New

<400> 30
Met Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn
 1               5                  10                  15      
Thr Asn Thr Ala Ile Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys
            20                  25                  30          
His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys
        35                  40                  45              
Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn
    50                  55                  60                  
Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu
65                  70                  75                  80  
Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly
                85                  90                  95      
Trp Arg Ile Ile Asn Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala
            100                 105                 110         
Ile Ala Ala Ile His Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe
        115                 120                 125             
Ser Tyr Asp Gly Ile Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn
    130                 135                 140                 
Asn Phe Tyr Phe Asp Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val
145                 150                 155                 160 
Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His
                165                 170                 175     
Asn Asn Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu
            180                 185                 190         
Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val
        195                 200                 205             
Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn
    210                 215                 220                 
Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr
225                 230                 235                 240 
Tyr Phe Asn Leu Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile
                245                 250                 255     
Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr
            260                 265                 270         
Gly Tyr Thr Ser Ile Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly
        275                 280                 285             
Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe
    290                 295                 300                 
Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Leu
305                 310                 315                 320 
Tyr Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly
                325                 330                 335     
Ser Asp Ser Lys Ala Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys
            340                 345                 350         
Tyr Tyr Phe Asn Thr Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr
        355                 360                 365             
Ile Asn Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser
    370                 375                 380                 
Thr Gly Tyr Thr Ile Ile Ser Gly Lys His Phe Tyr Phe Asn Thr Asp
385                 390                 395                 400 
Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr
                405                 410                 415     
Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile
            420                 425                 430         
Arg Tyr Gln Asn Arg Phe Leu Tyr Leu His Asp Asn Ile Tyr Tyr Phe
        435                 440                 445             
Gly Asn Asn Ser Lys Ala Ala Thr Gly Trp Val Thr Ile Asp Gly Asn
    450                 455                 460                 
Arg Tyr Tyr Phe Glu Pro Asn Thr Ala Met Gly Ala Asn Gly Tyr Lys
465                 470                 475                 480 
Thr Ile Asp Asn Lys Asn Phe Tyr Phe Arg Asn Gly Leu Pro Gln Ile
                485                 490                 495     
Gly Val Phe Lys Gly Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn
            500                 505                 510         
Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg
        515                 520                 525             
Phe Leu His Leu Leu Gly Lys Ile Tyr Tyr Phe Gly Asn Asn Ser Lys
    530                 535                 540                 
Ala Val Thr Gly Trp Gln Thr Ile Asn Gly Lys Val Tyr Tyr Phe Met
545                 550                 555                 560 
Pro Asp Thr Ala Met Ala Ala Ala Gly Gly Leu Phe Glu Ile Asp Gly
                565                 570                 575     
Val Ile Tyr Phe Phe Gly Val Asp Gly Val Lys Ala Val Lys Gly Leu
            580                 585                 590         
Asn Gln Ile Gly Asp Tyr Lys Tyr Tyr Phe Asn Ser Asp Gly Val Met
        595                 600                 605             
Gln Lys Gly Phe Val Ser Ile Asn Asp Asn Lys His Tyr Phe Asp Asp
    610                 615                 620                 
Ser Gly Val Met Lys Val Gly Tyr Thr Glu Ile Asp Gly Lys His Phe
625                 630                 635                 640 
Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile Gly Val Phe Asn Thr Glu
                645                 650                 655     
Asp Gly Phe Lys Tyr Phe Ala His His Asn Glu Asp Leu Gly Asn Glu
            660                 665                 670         
Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile Leu Asn Phe Asn Asn Lys
        675                 680                 685             
Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala Val Val Gly Trp Lys Asp
    690                 695                 700                 
Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp Glu Asp Thr Ala Glu Ala
705                 710                 715                 720 
Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly Gln Tyr Tyr Phe Asn Asp
                725                 730                 735     
Asp Gly Ile Met Gln Val Gly Phe Val Thr Ile Asn Asp Lys Val Phe
            740                 745                 750         
Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser Gly Val Gln Asn Ile Asp
        755                 760                 765             
Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly Ile Val Gln Ile Gly Val
    770                 775                 780                 
Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe Ala Pro Ala Asn Thr Val
785                 790                 795                 800 
Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu Tyr Ser Gly Leu Val Arg
                805                 810                 815     
Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu Thr Tyr Thr Ile Glu Thr
            820                 825                 830         
Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser Asp Lys Tyr Tyr Phe Asn
        835                 840                 845             
Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile Asn Leu Ile Asp Asp Ile
    850                 855                 860                 
Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met Arg Thr Gly Leu Ile Ser
865                 870                 875                 880 
Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu Asn Gly Glu Met Gln Phe
                885                 890                 895     
Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe Tyr Phe Gly Glu Asp Gly
            900                 905                 910         
Val Met Gln Ile Gly Val Phe Asn Thr Pro Asp Gly Phe Lys Tyr Phe
        915                 920                 925             
Ala His Gln Asn Thr Leu Asp Glu Asn Phe Glu Gly Glu Ser Ile Asn
    930                 935                 940                 
Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys Arg Tyr Tyr Phe Thr Asp
945                 950                 955                 960 
Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile Ile Asp Gly Glu Glu Tyr
                965                 970                 975     
Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val Ile Ser Glu His His His
            980                 985                 990         
His His His
        995 


<210> 31
<211> 2710
<212> PRT
<213> Clostridium Difficile

<400> 31
Met Ser Leu Ile Ser Lys Glu Glu Leu Ile Lys Leu Ala Tyr Ser Ile
 1               5                  10                  15      
Arg Pro Arg Glu Asn Glu Tyr Lys Thr Ile Leu Thr Asn Leu Asp Glu
            20                  25                  30          
Tyr Asn Lys Leu Thr Thr Asn Asn Asn Glu Asn Lys Tyr Leu Gln Leu
        35                  40                  45              
Lys Lys Leu Asn Glu Ser Ile Asp Val Phe Met Asn Lys Tyr Lys Thr
    50                  55                  60                  
Ser Ser Arg Asn Arg Ala Leu Ser Asn Leu Lys Lys Asp Ile Leu Lys
65                  70                  75                  80  
Glu Val Ile Leu Ile Lys Asn Ser Asn Thr Ser Pro Val Glu Lys Asn
                85                  90                  95      
Leu His Phe Val Trp Ile Gly Gly Glu Val Ser Asp Ile Ala Leu Glu
            100                 105                 110         
Tyr Ile Lys Gln Trp Ala Asp Ile Asn Ala Glu Tyr Asn Ile Lys Leu
        115                 120                 125             
Trp Tyr Asp Ser Glu Ala Phe Leu Val Asn Thr Leu Lys Lys Ala Ile
    130                 135                 140                 
Val Glu Ser Ser Thr Thr Glu Ala Leu Gln Leu Leu Glu Glu Glu Ile
145                 150                 155                 160 
Gln Asn Pro Gln Phe Asp Asn Met Lys Phe Tyr Lys Lys Arg Met Glu
                165                 170                 175     
Phe Ile Tyr Asp Arg Gln Lys Arg Phe Ile Asn Tyr Tyr Lys Ser Gln
            180                 185                 190         
Ile Asn Lys Pro Thr Val Pro Thr Ile Asp Asp Ile Ile Lys Ser His
        195                 200                 205             
Leu Val Ser Glu Tyr Asn Arg Asp Glu Thr Val Leu Glu Ser Tyr Arg
    210                 215                 220                 
Thr Asn Ser Leu Arg Lys Ile Asn Ser Asn His Gly Ile Asp Ile Arg
225                 230                 235                 240 
Ala Asn Ser Leu Phe Thr Glu Gln Glu Leu Leu Asn Ile Tyr Ser Gln
                245                 250                 255     
Glu Leu Leu Asn Arg Gly Asn Leu Ala Ala Ala Ser Asp Ile Val Arg
            260                 265                 270         
Leu Leu Ala Leu Lys Asn Phe Gly Gly Val Tyr Leu Asp Val Asp Met
        275                 280                 285             
Leu Pro Gly Ile His Ser Asp Leu Phe Lys Thr Ile Ser Arg Pro Ser
    290                 295                 300                 
Ser Ile Gly Leu Asp Arg Trp Glu Met Ile Lys Leu Glu Ala Ile Met
305                 310                 315                 320 
Lys Tyr Lys Lys Tyr Ile Asn Asn Tyr Thr Ser Glu Asn Phe Asp Lys
                325                 330                 335     
Leu Asp Gln Gln Leu Lys Asp Asn Phe Lys Leu Ile Ile Glu Ser Lys
            340                 345                 350         
Ser Glu Lys Ser Glu Ile Phe Ser Lys Leu Glu Asn Leu Asn Val Ser
        355                 360                 365             
Asp Leu Glu Ile Lys Ile Ala Phe Ala Leu Gly Ser Val Ile Asn Gln
    370                 375                 380                 
Ala Leu Ile Ser Lys Gln Gly Ser Tyr Leu Thr Asn Leu Val Ile Glu
385                 390                 395                 400 
Gln Val Lys Asn Arg Tyr Gln Phe Leu Asn Gln His Leu Asn Pro Ala
                405                 410                 415     
Ile Glu Ser Asp Asn Asn Phe Thr Asp Thr Thr Lys Ile Phe His Asp
            420                 425                 430         
Ser Leu Phe Asn Ser Ala Thr Ala Glu Asn Ser Met Phe Leu Thr Lys
        435                 440                 445             
Ile Ala Pro Tyr Leu Gln Val Gly Phe Met Pro Glu Ala Arg Ser Thr
    450                 455                 460                 
Ile Ser Leu Ser Gly Pro Gly Ala Tyr Ala Ser Ala Tyr Tyr Asp Phe
465                 470                 475                 480 
Ile Asn Leu Gln Glu Asn Thr Ile Glu Lys Thr Leu Lys Ala Ser Asp
                485                 490                 495     
Leu Ile Glu Phe Lys Phe Pro Glu Asn Asn Leu Ser Gln Leu Thr Glu
            500                 505                 510         
Gln Glu Ile Asn Ser Leu Trp Ser Phe Asp Gln Ala Ser Ala Lys Tyr
        515                 520                 525             
Gln Phe Glu Lys Tyr Val Arg Asp Tyr Thr Gly Gly Ser Leu Ser Glu
    530                 535                 540                 
Asp Asn Gly Val Asp Phe Asn Lys Asn Thr Ala Leu Asp Lys Asn Tyr
545                 550                 555                 560 
Leu Leu Asn Asn Lys Ile Pro Ser Asn Asn Val Glu Glu Ala Gly Ser
                565                 570                 575     
Lys Asn Tyr Val His Tyr Ile Ile Gln Leu Gln Gly Asp Asp Ile Ser
            580                 585                 590         
Tyr Glu Ala Thr Cys Asn Leu Phe Ser Lys Asn Pro Lys Asn Ser Ile
        595                 600                 605             
Ile Ile Gln Arg Asn Met Asn Glu Ser Ala Lys Ser Tyr Phe Leu Ser
    610                 615                 620                 
Asp Asp Gly Glu Ser Ile Leu Glu Leu Asn Lys Tyr Arg Ile Pro Glu
625                 630                 635                 640 
Arg Leu Lys Asn Lys Glu Lys Val Lys Val Thr Phe Ile Gly His Gly
                645                 650                 655     
Lys Asp Glu Phe Asn Thr Ser Glu Phe Ala Arg Leu Ser Val Asp Ser
            660                 665                 670         
Leu Ser Asn Glu Ile Ser Ser Phe Leu Asp Thr Ile Lys Leu Asp Ile
        675                 680                 685             
Ser Pro Lys Asn Val Glu Val Asn Leu Leu Gly Cys Asn Met Phe Ser
    690                 695                 700                 
Tyr Asp Phe Asn Val Glu Glu Thr Tyr Pro Gly Lys Leu Leu Leu Ser
705                 710                 715                 720 
Ile Met Asp Lys Ile Thr Ser Thr Leu Pro Asp Val Asn Lys Asn Ser
                725                 730                 735     
Ile Thr Ile Gly Ala Asn Gln Tyr Glu Val Arg Ile Asn Ser Glu Gly
            740                 745                 750         
Arg Lys Glu Leu Leu Ala His Ser Gly Lys Trp Ile Asn Lys Glu Glu
        755                 760                 765             
Ala Ile Met Ser Asp Leu Ser Ser Lys Glu Tyr Ile Phe Phe Asp Ser
    770                 775                 780                 
Ile Asp Asn Lys Leu Lys Ala Lys Ser Lys Asn Ile Pro Gly Leu Ala
785                 790                 795                 800 
Ser Ile Ser Glu Asp Ile Lys Thr Leu Leu Leu Asp Ala Ser Val Ser
                805                 810                 815     
Pro Asp Thr Lys Phe Ile Leu Asn Asn Leu Lys Leu Asn Ile Glu Ser
            820                 825                 830         
Ser Ile Gly Asp Tyr Ile Tyr Tyr Glu Lys Leu Glu Pro Val Lys Asn
        835                 840                 845             
Ile Ile His Asn Ser Ile Asp Asp Leu Ile Asp Glu Phe Asn Leu Leu
    850                 855                 860                 
Glu Asn Val Ser Asp Glu Leu Tyr Glu Leu Lys Lys Leu Asn Asn Leu
865                 870                 875                 880 
Asp Glu Lys Tyr Leu Ile Ser Phe Glu Asp Ile Ser Lys Asn Asn Ser
                885                 890                 895     
Thr Tyr Ser Val Arg Phe Ile Asn Lys Ser Asn Gly Glu Ser Val Tyr
            900                 905                 910         
Val Glu Thr Glu Lys Glu Ile Phe Ser Lys Tyr Ser Glu His Ile Thr
        915                 920                 925             
Lys Glu Ile Ser Thr Ile Lys Asn Ser Ile Ile Thr Asp Val Asn Gly
    930                 935                 940                 
Asn Leu Leu Asp Asn Ile Gln Leu Asp His Thr Ser Gln Val Asn Thr
945                 950                 955                 960 
Leu Asn Ala Ala Phe Phe Ile Gln Ser Leu Ile Asp Tyr Ser Ser Asn
                965                 970                 975     
Lys Asp Val Leu Asn Asp Leu Ser Thr Ser Val Lys Val Gln Leu Tyr
            980                 985                 990         
Ala Gln Leu Phe Ser Thr Gly Leu Asn Thr Ile Tyr Asp Ser Ile Gln
        995                 1000                1005            
Leu Val Asn Leu Ile Ser Asn Ala Val Asn Asp Thr Ile Asn Val Leu
    1010                1015                1020                
Pro Thr Ile Thr Glu Gly Ile Pro Ile Val Ser Thr Ile Leu Asp Gly
1025                1030                1035                1040
Ile Asn Leu Gly Ala Ala Ile Lys Glu Leu Leu Asp Glu His Asp Pro
                1045                1050                1055    
Leu Leu Lys Lys Glu Leu Glu Ala Lys Val Gly Val Leu Ala Ile Asn
            1060                1065                1070        
Met Ser Leu Ser Ile Ala Ala Thr Val Ala Ser Ile Val Gly Ile Gly
        1075                1080                1085            
Ala Glu Val Thr Ile Phe Leu Leu Pro Ile Ala Gly Ile Ser Ala Gly
    1090                1095                1100                
Ile Pro Ser Leu Val Asn Asn Glu Leu Ile Leu His Asp Lys Ala Thr
1105                1110                1115                1120
Ser Val Val Asn Tyr Phe Asn His Leu Ser Glu Ser Lys Lys Tyr Gly
                1125                1130                1135    
Pro Leu Lys Thr Glu Asp Asp Lys Ile Leu Val Pro Ile Asp Asp Leu
            1140                1145                1150        
Val Ile Ser Glu Ile Asp Phe Asn Asn Asn Ser Ile Lys Leu Gly Thr
        1155                1160                1165            
Cys Asn Ile Leu Ala Met Glu Gly Gly Ser Gly His Thr Val Thr Gly
    1170                1175                1180                
Asn Ile Asp His Phe Phe Ser Ser Pro Ser Ile Ser Ser His Ile Pro
1185                1190                1195                1200
Ser Leu Ser Ile Tyr Ser Ala Ile Gly Ile Glu Thr Glu Asn Leu Asp
                1205                1210                1215    
Phe Ser Lys Lys Ile Met Met Leu Pro Asn Ala Pro Ser Arg Val Phe
            1220                1225                1230        
Trp Trp Glu Thr Gly Ala Val Pro Gly Leu Arg Ser Leu Glu Asn Asp
        1235                1240                1245            
Gly Thr Arg Leu Leu Asp Ser Ile Arg Asp Leu Tyr Pro Gly Lys Phe
    1250                1255                1260                
Tyr Trp Arg Phe Tyr Ala Phe Phe Asp Tyr Ala Ile Thr Thr Leu Lys
1265                1270                1275                1280
Pro Val Tyr Glu Asp Thr Asn Ile Lys Ile Lys Leu Asp Lys Asp Thr
                1285                1290                1295    
Arg Asn Phe Ile Met Pro Thr Ile Thr Thr Asn Glu Ile Arg Asn Lys
            1300                1305                1310        
Leu Ser Tyr Ser Phe Asp Gly Ala Gly Gly Thr Tyr Ser Leu Leu Leu
        1315                1320                1325            
Ser Ser Tyr Pro Ile Ser Thr Asn Ile Asn Leu Ser Lys Asp Asp Leu
    1330                1335                1340                
Trp Ile Phe Asn Ile Asp Asn Glu Val Arg Glu Ile Ser Ile Glu Asn
1345                1350                1355                1360
Gly Thr Ile Lys Lys Gly Lys Leu Ile Lys Asp Val Leu Ser Lys Ile
                1365                1370                1375    
Asp Ile Asn Lys Asn Lys Leu Ile Ile Gly Asn Gln Thr Ile Asp Phe
            1380                1385                1390        
Ser Gly Asp Ile Asp Asn Lys Asp Arg Tyr Ile Phe Leu Thr Cys Glu
        1395                1400                1405            
Leu Asp Asp Lys Ile Ser Leu Ile Ile Glu Ile Asn Leu Val Ala Lys
    1410                1415                1420                
Ser Tyr Ser Leu Leu Leu Ser Gly Asp Lys Asn Tyr Leu Ile Ser Asn
1425                1430                1435                1440
Leu Ser Asn Thr Ile Glu Lys Ile Asn Thr Leu Gly Leu Asp Ser Lys
                1445                1450                1455    
Asn Ile Ala Tyr Asn Tyr Thr Asp Glu Ser Asn Asn Lys Tyr Phe Gly
            1460                1465                1470        
Ala Ile Ser Lys Thr Ser Gln Lys Ser Ile Ile His Tyr Lys Lys Asp
        1475                1480                1485            
Ser Lys Asn Ile Leu Glu Phe Tyr Asn Asp Ser Thr Leu Glu Phe Asn
    1490                1495                1500                
Ser Lys Asp Phe Ile Ala Glu Asp Ile Asn Val Phe Met Lys Asp Asp
1505                1510                1515                1520
Ile Asn Thr Ile Thr Gly Lys Tyr Tyr Val Asp Asn Asn Thr Asp Lys
                1525                1530                1535    
Ser Ile Asp Phe Ser Ile Ser Leu Val Ser Lys Asn Gln Val Lys Val
            1540                1545                1550        
Asn Gly Leu Tyr Leu Asn Glu Ser Val Tyr Ser Ser Tyr Leu Asp Phe
        1555                1560                1565            
Val Lys Asn Ser Asp Gly His His Asn Thr Ser Asn Phe Met Asn Leu
    1570                1575                1580                
Phe Leu Asp Asn Ile Ser Phe Trp Lys Leu Phe Gly Phe Glu Asn Ile
1585                1590                1595                1600
Asn Phe Val Ile Asp Lys Tyr Phe Thr Leu Val Gly Lys Thr Asn Leu
                1605                1610                1615    
Gly Tyr Val Glu Phe Ile Cys Asp Asn Asn Lys Asn Ile Asp Ile Tyr
            1620                1625                1630        
Phe Gly Glu Trp Lys Thr Ser Ser Ser Lys Ser Thr Ile Phe Ser Gly
        1635                1640                1645            
Asn Gly Arg Asn Val Val Val Glu Pro Ile Tyr Asn Pro Asp Thr Gly
    1650                1655                1660                
Glu Asp Ile Ser Thr Ser Leu Asp Phe Ser Tyr Glu Pro Leu Tyr Gly
1665                1670                1675                1680
Ile Asp Arg Tyr Ile Asn Lys Val Leu Ile Ala Pro Asp Leu Tyr Thr
                1685                1690                1695    
Ser Leu Ile Asn Ile Asn Thr Asn Tyr Tyr Ser Asn Glu Tyr Tyr Pro
            1700                1705                1710        
Glu Ile Ile Val Leu Asn Pro Asn Thr Phe His Lys Lys Val Asn Ile
        1715                1720                1725            
Asn Leu Asp Ser Ser Ser Phe Glu Tyr Lys Trp Ser Thr Glu Gly Ser
    1730                1735                1740                
Asp Phe Ile Leu Val Arg Tyr Leu Glu Glu Ser Asn Lys Lys Ile Leu
1745                1750                1755                1760
Gln Lys Ile Arg Ile Lys Gly Ile Leu Ser Asn Thr Gln Ser Phe Asn
                1765                1770                1775    
Lys Met Ser Ile Asp Phe Lys Asp Ile Lys Lys Leu Ser Leu Gly Tyr
            1780                1785                1790        
Ile Met Ser Asn Phe Lys Ser Phe Asn Ser Glu Asn Glu Leu Asp Arg
        1795                1800                1805            
Asp His Leu Gly Phe Lys Ile Ile Asp Asn Lys Thr Tyr Tyr Tyr Asp
    1810                1815                1820                
Glu Asp Ser Lys Leu Val Lys Gly Leu Ile Asn Ile Asn Asn Ser Leu
1825                1830                1835                1840
Phe Tyr Phe Asp Pro Ile Glu Phe Asn Leu Val Thr Gly Trp Gln Thr
                1845                1850                1855    
Ile Asn Gly Lys Lys Tyr Tyr Phe Asp Ile Asn Thr Gly Ala Ala Leu
            1860                1865                1870        
Thr Ser Tyr Lys Ile Ile Asn Gly Lys His Phe Tyr Phe Asn Asn Asp
        1875                1880                1885            
Gly Val Met Gln Leu Gly Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr
    1890                1895                1900                
Phe Ala Pro Ala Asn Thr Gln Asn Asn Asn Ile Glu Gly Gln Ala Ile
1905                1910                1915                1920
Val Tyr Gln Ser Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe
                1925                1930                1935    
Asp Asn Asn Ser Lys Ala Val Thr Gly Trp Arg Ile Ile Asn Asn Glu
            1940                1945                1950        
Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala Ala Val Gly Leu Gln
        1955                1960                1965            
Val Ile Asp Asn Asn Lys Tyr Tyr Phe Asn Pro Asp Thr Ala Ile Ile
    1970                1975                1980                
Ser Lys Gly Trp Gln Thr Val Asn Gly Ser Arg Tyr Tyr Phe Asp Thr
1985                1990                1995                2000
Asp Thr Ala Ile Ala Phe Asn Gly Tyr Lys Thr Ile Asp Gly Lys His
                2005                2010                2015    
Phe Tyr Phe Asp Ser Asp Cys Val Val Lys Ile Gly Val Phe Ser Thr
            2020                2025                2030        
Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Tyr Asn Asn Asn
        2035                2040                2045            
Ile Glu Gly Gln Ala Ile Val Tyr Gln Ser Lys Phe Leu Thr Leu Asn
    2050                2055                2060                
Gly Lys Lys Tyr Tyr Phe Asp Asn Asn Ser Lys Ala Val Thr Gly Leu
2065                2070                2075                2080
Gln Thr Ile Asp Ser Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Glu
                2085                2090                2095    
Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn
            2100                2105                2110        
Thr Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys
        2115                2120                2125            
Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Ile Ala Ser Thr Gly Tyr Thr
    2130                2135                2140                
Ile Ile Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met Gln
2145                2150                2155                2160
Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala
                2165                2170                2175    
Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln Asn
            2180                2185                2190        
Glu Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp Ser
        2195                2200                2205            
Lys Ala Val Thr Gly Trp Arg Ile Ile Asn Asn Lys Lys Tyr Tyr Phe
    2210                2215                2220                
Asn Pro Asn Asn Ala Ile Ala Ala Ile His Leu Cys Thr Ile Asn Asn
2225                2230                2235                2240
Asp Lys Tyr Tyr Phe Ser Tyr Asp Gly Ile Leu Gln Asn Gly Tyr Ile
                2245                2250                2255    
Thr Ile Glu Arg Asn Asn Phe Tyr Phe Asp Ala Asn Asn Glu Ser Lys
            2260                2265                2270        
Met Val Thr Gly Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala
        2275                2280                2285            
Pro Ala Asn Thr His Asn Asn Asn Ile Glu Gly Gln Ala Ile Val Tyr
    2290                2295                2300                
Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Asp Asn
2305                2310                2315                2320
Asp Ser Lys Ala Val Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr
                2325                2330                2335    
Tyr Phe Asn Leu Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile
            2340                2345                2350        
Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala Glu Ala Ala Thr
        2355                2360                2365            
Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr
    2370                2375                2380                
Phe Ile Ala Ser Thr Gly Tyr Thr Ser Ile Asn Gly Lys His Phe Tyr
2385                2390                2395                2400
Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro Asn
                2405                2410                2415    
Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu
            2420                2425                2430        
Gly Gln Ala Ile Leu Tyr Gln Asn Lys Phe Leu Thr Leu Asn Gly Lys
        2435                2440                2445            
Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Leu Arg Thr
    2450                2455                2460                
Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ala Val Ala Val
2465                2470                2475                2480
Thr Gly Trp Gln Thr Ile Asn Gly Lys Lys Tyr Tyr Phe Asn Thr Asn
                2485                2490                2495    
Thr Ser Ile Ala Ser Thr Gly Tyr Thr Ile Ile Ser Gly Lys His Phe
            2500                2505                2510        
Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro
        2515                2520                2525            
Asp Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile
    2530                2535                2540                
Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg Phe Leu Tyr Leu His Asp
2545                2550                2555                2560
Asn Ile Tyr Tyr Phe Gly Asn Asn Ser Lys Ala Ala Thr Gly Trp Val
                2565                2570                2575    
Thr Ile Asp Gly Asn Arg Tyr Tyr Phe Glu Pro Asn Thr Ala Met Gly
            2580                2585                2590        
Ala Asn Gly Tyr Lys Thr Ile Asp Asn Lys Asn Phe Tyr Phe Arg Asn
        2595                2600                2605            
Gly Leu Pro Gln Ile Gly Val Phe Lys Gly Ser Asn Gly Phe Glu Tyr
    2610                2615                2620                
Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile
2625                2630                2635                2640
Arg Tyr Gln Asn Arg Phe Leu His Leu Leu Gly Lys Ile Tyr Tyr Phe
                2645                2650                2655    
Gly Asn Asn Ser Lys Ala Val Thr Gly Trp Gln Thr Ile Asn Gly Lys
            2660                2665                2670        
Val Tyr Tyr Phe Met Pro Asp Thr Ala Met Ala Ala Ala Gly Gly Leu
        2675                2680                2685            
Phe Glu Ile Asp Gly Val Ile Tyr Phe Phe Gly Val Asp Gly Val Lys
    2690                2695                2700                
Ala Pro Gly Ile Tyr Gly
2705                2710


<210> 32
<211> 2366
<212> PRT
<213> Clostridium difficile

<400> 32
Met Ser Leu Val Asn Arg Lys Gln Leu Glu Lys Met Ala Asn Val Arg
 1               5                  10                  15      
Phe Arg Thr Gln Glu Asp Glu Tyr Val Ala Ile Leu Asp Ala Leu Glu
            20                  25                  30          
Glu Tyr His Asn Met Ser Glu Asn Thr Val Val Glu Lys Tyr Leu Lys
        35                  40                  45              
Leu Lys Asp Ile Asn Ser Leu Thr Asp Ile Tyr Ile Asp Thr Tyr Lys
    50                  55                  60                  
Lys Ser Gly Arg Asn Lys Ala Leu Lys Lys Phe Lys Glu Tyr Leu Val
65                  70                  75                  80  
Thr Glu Val Leu Glu Leu Lys Asn Asn Asn Leu Thr Pro Val Glu Lys
                85                  90                  95      
Asn Leu His Phe Val Trp Ile Gly Gly Gln Ile Asn Asp Thr Ala Ile
            100                 105                 110         
Asn Tyr Ile Asn Gln Trp Lys Asp Val Asn Ser Asp Tyr Asn Val Asn
        115                 120                 125             
Val Phe Tyr Asp Ser Asn Ala Phe Leu Ile Asn Thr Leu Lys Lys Thr
    130                 135                 140                 
Val Val Glu Ser Ala Ile Asn Asp Thr Leu Glu Ser Phe Arg Glu Asn
145                 150                 155                 160 
Leu Asn Asp Pro Arg Phe Asp Tyr Asn Lys Phe Phe Arg Lys Arg Met
                165                 170                 175     
Glu Ile Ile Tyr Asp Lys Gln Lys Asn Phe Ile Asn Tyr Tyr Lys Ala
            180                 185                 190         
Gln Arg Glu Glu Asn Pro Glu Leu Ile Ile Asp Asp Ile Val Lys Thr
        195                 200                 205             
Tyr Leu Ser Asn Glu Tyr Ser Lys Glu Ile Asp Glu Leu Asn Thr Tyr
    210                 215                 220                 
Ile Glu Glu Ser Leu Asn Lys Ile Thr Gln Asn Ser Gly Asn Asp Val
225                 230                 235                 240 
Arg Asn Phe Glu Glu Phe Lys Asn Gly Glu Ser Phe Asn Leu Tyr Glu
                245                 250                 255     
Gln Glu Leu Val Glu Arg Trp Asn Leu Ala Ala Ala Ser Asp Ile Leu
            260                 265                 270         
Arg Ile Ser Ala Leu Lys Glu Ile Gly Gly Met Tyr Leu Asp Val Asp
        275                 280                 285             
Met Leu Pro Gly Ile Gln Pro Asp Leu Phe Glu Ser Ile Glu Lys Pro
    290                 295                 300                 
Ser Ser Val Thr Val Asp Phe Trp Glu Met Thr Lys Leu Glu Ala Ile
305                 310                 315                 320 
Met Lys Tyr Lys Glu Tyr Ile Pro Glu Tyr Thr Ser Glu His Phe Asp
                325                 330                 335     
Met Leu Asp Glu Glu Val Gln Ser Ser Phe Glu Ser Val Leu Ala Ser
            340                 345                 350         
Lys Ser Asp Lys Ser Glu Ile Phe Ser Ser Leu Gly Asp Met Glu Ala
        355                 360                 365             
Ser Pro Leu Glu Val Lys Ile Ala Phe Asn Ser Lys Gly Ile Ile Asn
    370                 375                 380                 
Gln Gly Leu Ile Ser Val Lys Asp Ser Tyr Cys Ser Asn Leu Ile Val
385                 390                 395                 400 
Lys Gln Ile Glu Asn Arg Tyr Lys Ile Leu Asn Asn Ser Leu Asn Pro
                405                 410                 415     
Ala Ile Ser Glu Asp Asn Asp Phe Asn Thr Thr Thr Asn Thr Phe Ile
            420                 425                 430         
Asp Ser Ile Met Ala Glu Ala Asn Ala Asp Asn Gly Arg Phe Met Met
        435                 440                 445             
Glu Leu Gly Lys Tyr Leu Arg Val Gly Phe Phe Pro Asp Val Lys Thr
    450                 455                 460                 
Thr Ile Asn Leu Ser Gly Pro Glu Ala Tyr Ala Ala Ala Tyr Gln Asp
465                 470                 475                 480 
Leu Leu Met Phe Lys Glu Gly Ser Met Asn Ile His Leu Ile Glu Ala
                485                 490                 495     
Asp Leu Arg Asn Phe Glu Ile Ser Lys Thr Asn Ile Ser Gln Ser Thr
            500                 505                 510         
Glu Gln Glu Met Ala Ser Leu Trp Ser Phe Asp Asp Ala Arg Ala Lys
        515                 520                 525             
Ala Gln Phe Glu Glu Tyr Lys Arg Asn Tyr Phe Glu Gly Ser Leu Gly
    530                 535                 540                 
Glu Asp Asp Asn Leu Asp Phe Ser Gln Asn Ile Val Val Asp Lys Glu
545                 550                 555                 560 
Tyr Leu Leu Glu Lys Ile Ser Ser Leu Ala Arg Ser Ser Glu Arg Gly
                565                 570                 575     
Tyr Ile His Tyr Ile Val Gln Leu Gln Gly Asp Lys Ile Ser Tyr Glu
            580                 585                 590         
Ala Ala Cys Asn Leu Phe Ala Lys Thr Pro Tyr Asp Ser Val Leu Phe
        595                 600                 605             
Gln Lys Asn Ile Glu Asp Ser Glu Ile Ala Tyr Tyr Tyr Asn Pro Gly
    610                 615                 620                 
Asp Gly Glu Ile Gln Glu Ile Asp Lys Tyr Lys Ile Pro Ser Ile Ile
625                 630                 635                 640 
Ser Asp Arg Pro Lys Ile Lys Leu Thr Phe Ile Gly His Gly Lys Asp
                645                 650                 655     
Glu Phe Asn Thr Asp Ile Phe Ala Gly Phe Asp Val Asp Ser Leu Ser
            660                 665                 670         
Thr Glu Ile Glu Ala Ala Ile Asp Leu Ala Lys Glu Asp Ile Ser Pro
        675                 680                 685             
Lys Ser Ile Glu Ile Asn Leu Leu Gly Cys Asn Met Phe Ser Tyr Ser
    690                 695                 700                 
Ile Asn Val Glu Glu Thr Tyr Pro Gly Lys Leu Leu Leu Lys Val Lys
705                 710                 715                 720 
Asp Lys Ile Ser Glu Leu Met Pro Ser Ile Ser Gln Asp Ser Ile Ile
                725                 730                 735     
Val Ser Ala Asn Gln Tyr Glu Val Arg Ile Asn Ser Glu Gly Arg Arg
            740                 745                 750         
Glu Leu Leu Asp His Ser Gly Glu Trp Ile Asn Lys Glu Glu Ser Ile
        755                 760                 765             
Ile Lys Asp Ile Ser Ser Lys Glu Tyr Ile Ser Phe Asn Pro Lys Glu
    770                 775                 780                 
Asn Lys Ile Thr Val Lys Ser Lys Asn Leu Pro Glu Leu Ser Thr Leu
785                 790                 795                 800 
Leu Gln Glu Ile Arg Asn Asn Ser Asn Ser Ser Asp Ile Glu Leu Glu
                805                 810                 815     
Glu Lys Val Met Leu Thr Glu Cys Glu Ile Asn Val Ile Ser Asn Ile
            820                 825                 830         
Asp Thr Gln Ile Val Glu Glu Arg Ile Glu Glu Ala Lys Asn Leu Thr
        835                 840                 845             
Ser Asp Ser Ile Asn Tyr Ile Lys Asp Glu Phe Lys Leu Ile Glu Ser
    850                 855                 860                 
Ile Ser Asp Ala Leu Cys Asp Leu Lys Gln Gln Asn Glu Leu Glu Asp
865                 870                 875                 880 
Ser His Phe Ile Ser Phe Glu Asp Ile Ser Glu Thr Asp Glu Gly Phe
                885                 890                 895     
Ser Ile Arg Phe Ile Asn Lys Glu Thr Gly Glu Ser Ile Phe Val Glu
            900                 905                 910         
Thr Glu Lys Thr Ile Phe Ser Glu Tyr Ala Asn His Ile Thr Glu Glu
        915                 920                 925             
Ile Ser Lys Ile Lys Gly Thr Ile Phe Asp Thr Val Asn Gly Lys Leu
    930                 935                 940                 
Val Lys Lys Val Asn Leu Asp Thr Thr His Glu Val Asn Thr Leu Asn
945                 950                 955                 960 
Ala Ala Phe Phe Ile Gln Ser Leu Ile Glu Tyr Asn Ser Ser Lys Glu
                965                 970                 975     
Ser Leu Ser Asn Leu Ser Val Ala Met Lys Val Gln Val Tyr Ala Gln
            980                 985                 990         
Leu Phe Ser Thr Gly Leu Asn Thr Ile Thr Asp Ala Ala Lys Val Val
        995                 1000                1005            
Glu Leu Val Ser Thr Ala Leu Asp Glu Thr Ile Asp Leu Leu Pro Thr
    1010                1015                1020                
Leu Ser Glu Gly Leu Pro Ile Ile Ala Thr Ile Ile Asp Gly Val Ser
1025                1030                1035                1040
Leu Gly Ala Ala Ile Lys Glu Leu Ser Glu Thr Ser Asp Pro Leu Leu
                1045                1050                1055    
Arg Gln Glu Ile Glu Ala Lys Ile Gly Ile Met Ala Val Asn Leu Thr
            1060                1065                1070        
Thr Ala Thr Thr Ala Ile Ile Thr Ser Ser Leu Gly Ile Ala Ser Gly
        1075                1080                1085            
Phe Ser Ile Leu Leu Val Pro Leu Ala Gly Ile Ser Ala Gly Ile Pro
    1090                1095                1100                
Ser Leu Val Asn Asn Glu Leu Val Leu Arg Asp Lys Ala Thr Lys Val
1105                1110                1115                1120
Val Asp Tyr Phe Lys His Val Ser Leu Val Glu Thr Glu Gly Val Phe
                1125                1130                1135    
Thr Leu Leu Asp Asp Lys Ile Met Met Pro Gln Asp Asp Leu Val Ile
            1140                1145                1150        
Ser Glu Ile Asp Phe Asn Asn Asn Ser Ile Val Leu Gly Lys Cys Glu
        1155                1160                1165            
Ile Trp Arg Met Glu Gly Gly Ser Gly His Thr Val Thr Asp Asp Ile
    1170                1175                1180                
Asp His Phe Phe Ser Ala Pro Ser Ile Thr Tyr Arg Glu Pro His Leu
1185                1190                1195                1200
Ser Ile Tyr Asp Val Leu Glu Val Gln Lys Glu Glu Leu Asp Leu Ser
                1205                1210                1215    
Lys Asp Leu Met Val Leu Pro Asn Ala Pro Asn Arg Val Phe Ala Trp
            1220                1225                1230        
Glu Thr Gly Trp Thr Pro Gly Leu Arg Ser Leu Glu Asn Asp Gly Thr
        1235                1240                1245            
Lys Leu Leu Asp Arg Ile Arg Asp Asn Tyr Glu Gly Glu Phe Tyr Trp
    1250                1255                1260                
Arg Tyr Phe Ala Phe Ile Ala Asp Ala Leu Ile Thr Thr Leu Lys Pro
1265                1270                1275                1280
Arg Tyr Glu Asp Thr Asn Ile Arg Ile Asn Leu Asp Ser Asn Thr Arg
                1285                1290                1295    
Ser Phe Ile Val Pro Ile Ile Thr Thr Glu Tyr Ile Arg Glu Lys Leu
            1300                1305                1310        
Ser Tyr Ser Phe Tyr Gly Ser Gly Gly Thr Tyr Ala Leu Ser Leu Ser
        1315                1320                1325            
Gln Tyr Asn Met Gly Ile Asn Ile Glu Leu Ser Glu Ser Asp Val Trp
    1330                1335                1340                
Ile Ile Asp Val Asp Asn Val Val Arg Asp Val Thr Ile Glu Ser Asp
1345                1350                1355                1360
Lys Ile Lys Lys Gly Asp Leu Ile Glu Gly Ile Leu Ser Thr Leu Ser
                1365                1370                1375    
Ile Glu Glu Asn Lys Ile Ile Leu Asn Ser His Glu Ile Asn Phe Ser
            1380                1385                1390        
Gly Glu Val Asn Gly Ser Asn Gly Phe Val Ser Leu Thr Phe Ser Ile
        1395                1400                1405            
Leu Glu Gly Ile Asn Ala Ile Ile Glu Val Asp Leu Leu Ser Lys Ser
    1410                1415                1420                
Tyr Lys Leu Leu Ile Ser Gly Glu Leu Lys Ile Leu Met Leu Asn Ser
1425                1430                1435                1440
Asn His Ile Gln Gln Lys Ile Asp Tyr Ile Gly Phe Asn Ser Glu Leu
                1445                1450                1455    
Gln Lys Asn Ile Pro Tyr Ser Phe Val Asp Ser Glu Gly Lys Glu Asn
            1460                1465                1470        
Gly Phe Ile Asn Gly Ser Thr Lys Glu Gly Leu Phe Val Ser Glu Leu
        1475                1480                1485            
Pro Asp Val Val Leu Ile Ser Lys Val Tyr Met Asp Asp Ser Lys Pro
    1490                1495                1500                
Ser Phe Gly Tyr Tyr Ser Asn Asn Leu Lys Asp Val Lys Val Ile Thr
1505                1510                1515                1520
Lys Asp Asn Val Asn Ile Leu Thr Gly Tyr Tyr Leu Lys Asp Asp Ile
                1525                1530                1535    
Lys Ile Ser Leu Ser Leu Thr Leu Gln Asp Glu Lys Thr Ile Lys Leu
            1540                1545                1550        
Asn Ser Val His Leu Asp Glu Ser Gly Val Ala Glu Ile Leu Lys Phe
        1555                1560                1565            
Met Asn Arg Lys Gly Asn Thr Asn Thr Ser Asp Ser Leu Met Ser Phe
    1570                1575                1580                
Leu Glu Ser Met Asn Ile Lys Ser Ile Phe Val Asn Phe Leu Gln Ser
1585                1590                1595                1600
Asn Ile Lys Phe Ile Leu Asp Ala Asn Phe Ile Ile Ser Gly Thr Thr
                1605                1610                1615    
Ser Ile Gly Gln Phe Glu Phe Ile Cys Asp Glu Asn Asp Asn Ile Gln
            1620                1625                1630        
Pro Tyr Phe Ile Lys Phe Asn Thr Leu Glu Thr Asn Tyr Thr Leu Tyr
        1635                1640                1645            
Val Gly Asn Arg Gln Asn Met Ile Val Glu Pro Asn Tyr Asp Leu Asp
    1650                1655                1660                
Asp Ser Gly Asp Ile Ser Ser Thr Val Ile Asn Phe Ser Gln Lys Tyr
1665                1670                1675                1680
Leu Tyr Gly Ile Asp Ser Cys Val Asn Lys Val Val Ile Ser Pro Asn
                1685                1690                1695    
Ile Tyr Thr Asp Glu Ile Asn Ile Thr Pro Val Tyr Glu Thr Asn Asn
            1700                1705                1710        
Thr Tyr Pro Glu Val Ile Val Leu Asp Ala Asn Tyr Ile Asn Glu Lys
        1715                1720                1725            
Ile Asn Val Asn Ile Asn Asp Leu Ser Ile Arg Tyr Val Trp Ser Asn
    1730                1735                1740                
Asp Gly Asn Asp Phe Ile Leu Met Ser Thr Ser Glu Glu Asn Lys Val
1745                1750                1755                1760
Ser Gln Val Lys Ile Arg Phe Val Asn Val Phe Lys Asp Lys Thr Leu
                1765                1770                1775    
Ala Asn Lys Leu Ser Phe Asn Phe Ser Asp Lys Gln Asp Val Pro Val
            1780                1785                1790        
Ser Glu Ile Ile Leu Ser Phe Thr Pro Ser Tyr Tyr Glu Asp Gly Leu
        1795                1800                1805            
Ile Gly Tyr Asp Leu Gly Leu Val Ser Leu Tyr Asn Glu Lys Phe Tyr
    1810                1815                1820                
Ile Asn Asn Phe Gly Met Met Val Ser Gly Leu Ile Tyr Ile Asn Asp
1825                1830                1835                1840
Ser Leu Tyr Tyr Phe Lys Pro Pro Val Asn Asn Leu Ile Thr Gly Phe
                1845                1850                1855    
Val Thr Val Gly Asp Asp Lys Tyr Tyr Phe Asn Pro Ile Asn Gly Gly
            1860                1865                1870        
Ala Ala Ser Ile Gly Glu Thr Ile Ile Asp Asp Lys Asn Tyr Tyr Phe
        1875                1880                1885            
Asn Gln Ser Gly Val Leu Gln Thr Gly Val Phe Ser Thr Glu Asp Gly
    1890                1895                1900                
Phe Lys Tyr Phe Ala Pro Ala Asn Thr Leu Asp Glu Asn Leu Glu Gly
1905                1910                1915                1920
Glu Ala Ile Asp Phe Thr Gly Lys Leu Ile Ile Asp Glu Asn Ile Tyr
                1925                1930                1935    
Tyr Phe Asp Asp Asn Tyr Arg Gly Ala Val Glu Trp Lys Glu Leu Asp
            1940                1945                1950        
Gly Glu Met His Tyr Phe Ser Pro Glu Thr Gly Lys Ala Phe Lys Gly
        1955                1960                1965            
Leu Asn Gln Ile Gly Asp Tyr Lys Tyr Tyr Phe Asn Ser Asp Gly Val
    1970                1975                1980                
Met Gln Lys Gly Phe Val Ser Ile Asn Asp Asn Lys His Tyr Phe Asp
1985                1990                1995                2000
Asp Ser Gly Val Met Lys Val Gly Tyr Thr Glu Ile Asp Gly Lys His
                2005                2010                2015    
Phe Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile Gly Val Phe Asn Thr
            2020                2025                2030        
Glu Asp Gly Phe Lys Tyr Phe Ala His His Asn Glu Asp Leu Gly Asn
        2035                2040                2045            
Glu Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile Leu Asn Phe Asn Asn
    2050                2055                2060                
Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala Val Val Gly Trp Lys
2065                2070                2075                2080
Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp Glu Asp Thr Ala Glu
                2085                2090                2095    
Ala Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly Gln Tyr Tyr Phe Asn
            2100                2105                2110        
Asp Asp Gly Ile Met Gln Val Gly Phe Val Thr Ile Asn Asp Lys Val
        2115                2120                2125            
Phe Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser Gly Val Gln Asn Ile
    2130                2135                2140                
Asp Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly Ile Val Gln Ile Gly
2145                2150                2155                2160
Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe Ala Pro Ala Asn Thr
                2165                2170                2175    
Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu Tyr Ser Gly Leu Val
            2180                2185                2190        
Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu Thr Tyr Thr Ile Glu
        2195                2200                2205            
Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser Asp Lys Tyr Tyr Phe
    2210                2215                2220                
Asn Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile Asn Leu Ile Asp Asp
2225                2230                2235                2240
Ile Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met Arg Thr Gly Leu Ile
                2245                2250                2255    
Ser Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu Asn Gly Glu Met Gln
            2260                2265                2270        
Phe Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe Tyr Phe Gly Glu Asp
        2275                2280                2285            
Gly Val Met Gln Ile Gly Val Phe Asn Thr Pro Asp Gly Phe Lys Tyr
    2290                2295                2300                
Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe Glu Gly Glu Ser Ile
2305                2310                2315                2320
Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys Arg Tyr Tyr Phe Thr
                2325                2330                2335    
Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile Ile Asp Gly Glu Glu
            2340                2345                2350        
Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val Ile Ser Glu
        2355                2360                2365    


<210> 33
<211> 667
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of CDTb" C39 when expressed in
      fusion with GST.

<400> 33
Leu Met Ser Asp Trp Glu Asp Glu Asp Leu Asp Thr Asp Asn Asp Asn
 1               5                  10                  15      
Ile Pro Asp Ser Tyr Glu Arg Asn Gly Tyr Thr Ile Lys Asp Leu Ile
            20                  25                  30          
Ala Val Lys Trp Glu Asp Ser Phe Ala Glu Gln Gly Tyr Lys Lys Tyr
        35                  40                  45              
Val Ser Asn Tyr Leu Glu Ser Asn Thr Ala Gly Asp Pro Tyr Thr Asp
    50                  55                  60                  
Tyr Glu Lys Ala Ser Gly Ser Phe Asp Lys Ala Ile Lys Thr Glu Ala
65                  70                  75                  80  
Arg Asp Pro Leu Val Ala Ala Tyr Pro Ile Val Gly Val Gly Met Glu
                85                  90                  95      
Lys Leu Ile Ile Ser Thr Asn Glu His Ala Ser Thr Asp Gln Gly Lys
            100                 105                 110         
Thr Val Ser Arg Ala Thr Thr Asn Ser Lys Thr Glu Ser Asn Thr Ala
        115                 120                 125             
Gly Val Ser Val Asn Val Gly Tyr Gln Asn Gly Phe Thr Ala Asn Val
    130                 135                 140                 
Thr Thr Asn Tyr Ser His Thr Thr Asp Asn Ser Thr Ala Val Gln Asp
145                 150                 155                 160 
Ser Asn Gly Glu Ser Trp Asn Thr Gly Leu Ser Ile Asn Lys Gly Glu
                165                 170                 175     
Ser Ala Tyr Ile Asn Ala Asn Val Arg Tyr Tyr Asn Thr Gly Thr Ala
            180                 185                 190         
Pro Met Tyr Lys Val Thr Pro Thr Thr Asn Leu Val Leu Asp Gly Asp
        195                 200                 205             
Thr Leu Ser Thr Ile Lys Ala Gln Glu Asn Gln Ile Gly Asn Asn Leu
    210                 215                 220                 
Ser Pro Gly Asp Thr Tyr Pro Lys Lys Gly Leu Ser Pro Leu Ala Leu
225                 230                 235                 240 
Asn Thr Met Asp Gln Phe Ser Ser Arg Leu Ile Pro Ile Asn Tyr Asp
                245                 250                 255     
Gln Leu Lys Lys Leu Asp Ala Gly Lys Gln Ile Lys Leu Glu Thr Thr
            260                 265                 270         
Gln Val Ser Gly Asn Phe Gly Thr Lys Asn Ser Ser Gly Gln Ile Val
        275                 280                 285             
Thr Glu Gly Asn Ser Trp Ser Asp Tyr Ile Ser Gln Ile Asp Ser Ile
    290                 295                 300                 
Ser Ala Ser Ile Ile Leu Asp Thr Glu Asn Glu Ser Tyr Glu Arg Arg
305                 310                 315                 320 
Val Thr Ala Lys Asn Leu Gln Asp Pro Glu Asp Lys Thr Pro Glu Leu
                325                 330                 335     
Thr Ile Gly Glu Ala Ile Glu Lys Ala Phe Gly Ala Thr Lys Lys Asp
            340                 345                 350         
Gly Leu Leu Tyr Phe Asn Asp Ile Pro Ile Asp Glu Ser Cys Val Glu
        355                 360                 365             
Leu Ile Phe Asp Asp Asn Thr Ala Asn Lys Ile Lys Asp Ser Leu Lys
    370                 375                 380                 
Thr Leu Ser Asp Lys Lys Ile Tyr Asn Val Lys Leu Glu Arg Gly Met
385                 390                 395                 400 
Asn Ile Leu Ile Lys Thr Pro Thr Tyr Phe Thr Asn Phe Asp Asp Tyr
                405                 410                 415     
Asn Asn Tyr Pro Ser Thr Trp Ser Asn Val Asn Thr Thr Asn Gln Asp
            420                 425                 430         
Gly Leu Gln Gly Ser Ala Asn Lys Leu Asn Gly Glu Thr Lys Ile Lys
        435                 440                 445             
Ile Pro Met Ser Glu Leu Lys Pro Tyr Lys Arg Tyr Val Phe Ser Gly
    450                 455                 460                 
Tyr Ser Lys Asp Pro Leu Thr Ser Asn Ser Ile Ile Val Lys Ile Lys
465                 470                 475                 480 
Ala Lys Glu Glu Lys Thr Asp Tyr Leu Val Pro Glu Gln Gly Tyr Thr
                485                 490                 495     
Lys Phe Ser Tyr Glu Phe Glu Thr Thr Glu Lys Asp Ser Ser Asn Ile
            500                 505                 510         
Glu Ile Thr Leu Ile Gly Ser Gly Thr Thr Tyr Leu Asp Asn Leu Ser
        515                 520                 525             
Ile Thr Glu Leu Asn Ser Thr Pro Glu Ile Leu Asp Glu Pro Glu Val
    530                 535                 540                 
Lys Ile Pro Thr Asp Gln Glu Ile Met Asp Ala His Lys Ile Tyr Phe
545                 550                 555                 560 
Ala Asp Leu Asn Phe Asn Pro Ser Thr Gly Asn Thr Tyr Ile Asn Gly
                565                 570                 575     
Met Tyr Phe Ala Pro Thr Gln Thr Asn Lys Glu Ala Leu Asp Tyr Ile
            580                 585                 590         
Gln Lys Tyr Arg Val Glu Ala Thr Leu Gln Tyr Ser Gly Phe Lys Asp
        595                 600                 605             
Ile Gly Thr Lys Asp Lys Glu Met Arg Asn Tyr Leu Gly Asp Pro Asn
    610                 615                 620                 
Gln Pro Lys Thr Asn Tyr Val Asn Leu Arg Ser Tyr Phe Thr Gly Gly
625                 630                 635                 640 
Glu Asn Ile Met Thr Tyr Lys Lys Leu Arg Ile Tyr Ala Ile Thr Pro
                645                 650                 655     
Asp Asp Arg Glu Leu Leu Val Leu Ser Val Asp
            660                 665         


<210> 34
<211> 266
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of CdtB receptor binding
      domain with linker in N-term of sequence, from aa
      620-876 (C52)

<400> 34
Met Thr Asn Phe Asp Asp Tyr Asn Asn Tyr Pro Ser Thr Trp Ser Asn
 1               5                  10                  15      
Val Asn Thr Thr Asn Gln Asp Gly Leu Gln Gly Ser Ala Asn Lys Leu
            20                  25                  30          
Asn Gly Glu Thr Lys Ile Lys Ile Pro Met Ser Glu Leu Lys Pro Tyr
        35                  40                  45              
Lys Arg Tyr Val Phe Ser Gly Tyr Ser Lys Asp Pro Leu Thr Ser Asn
    50                  55                  60                  
Ser Ile Ile Val Lys Ile Lys Ala Lys Glu Glu Lys Thr Asp Tyr Leu
65                  70                  75                  80  
Val Pro Glu Gln Gly Tyr Thr Lys Phe Ser Tyr Glu Phe Glu Thr Thr
                85                  90                  95      
Glu Lys Asp Ser Ser Asn Ile Glu Ile Thr Leu Ile Gly Ser Gly Thr
            100                 105                 110         
Thr Tyr Leu Asp Asn Leu Ser Ile Thr Glu Leu Asn Ser Thr Pro Glu
        115                 120                 125             
Ile Leu Asp Glu Pro Glu Val Lys Ile Pro Thr Asp Gln Glu Ile Met
    130                 135                 140                 
Asp Ala His Lys Ile Tyr Phe Ala Asp Leu Asn Phe Asn Pro Ser Thr
145                 150                 155                 160 
Gly Asn Thr Tyr Ile Asn Gly Met Tyr Phe Ala Pro Thr Gln Thr Asn
                165                 170                 175     
Lys Glu Ala Leu Asp Tyr Ile Gln Lys Tyr Arg Val Glu Ala Thr Leu
            180                 185                 190         
Gln Tyr Ser Gly Phe Lys Asp Ile Gly Thr Lys Asp Lys Glu Met Arg
        195                 200                 205             
Asn Tyr Leu Gly Asp Pro Asn Gln Pro Lys Thr Asn Tyr Val Asn Leu
    210                 215                 220                 
Arg Ser Tyr Phe Thr Gly Gly Glu Asn Ile Met Thr Tyr Lys Lys Leu
225                 230                 235                 240 
Arg Ile Tyr Ala Ile Thr Pro Asp Asp Arg Glu Leu Leu Val Leu Ser
                245                 250                 255     
Val Asp Gly Gly His His His His His His
            260                 265     


<210> 35
<211> 801
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence of C52

<400> 35
atgaccaatt ttgatgatta taacaattat ccgagcactt ggagcaatgt gaataccacc 60
aatcaggatg gtctgcaggg tagcgcaaat aaactgaatg gtgaaaccaa aatcaaaatt 120
ccgatgagcg aactgaaacc gtataaacgt tatgtgttta gcggctatag caaagatccg 180
ctgaccagca atagcattat tgtgaaaatc aaagccaaag aagaaaaaac cgattatctg 240
gttccggaac agggttatac caaatttagc tatgaatttg aaaccaccga aaaagatagc 300
agtaatattg aaattaccct gattggtagc ggcaccacct atctggataa tctgagtatt 360
accgaactga atagcacacc ggaaattctg gatgaaccgg aagtgaaaat tccgaccgat 420
caagaaatta tggatgccca taaaatctat tttgccgatc tgaactttaa tccgagcacc 480
ggcaatacct atattaacgg catgtatttt gcaccgaccc agaccaataa agaagccctg 540
gattatattc agaaatatcg tgttgaagcc accctgcagt atagcggttt taaagatatt 600
ggcaccaaag ataaagaaat gcgtaattat ctgggcgatc cgaatcagcc gaaaaccaat 660
tatgttaatc tgcgcagcta ttttaccggt ggcgaaaaca ttatgaccta caaaaaactg 720
cgcatttatg ccattacacc ggatgatcgt gaactgctgg ttctgagcgt tgatggcggt 780
caccaccatc atcatcatta a                                           801

<210> 36
<211> 250
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of CdtB receptor binding
      domain without linker in N-term of sequence, from
      aa  636-876 (C55)

<400> 36
Met Asn Thr Thr Asn Gln Asp Gly Leu Gln Gly Ser Ala Asn Lys Leu
 1               5                  10                  15      
Asn Gly Glu Thr Lys Ile Lys Ile Pro Met Ser Glu Leu Lys Pro Tyr
            20                  25                  30          
Lys Arg Tyr Val Phe Ser Gly Tyr Ser Lys Asp Pro Leu Thr Ser Asn
        35                  40                  45              
Ser Ile Ile Val Lys Ile Lys Ala Lys Glu Glu Lys Thr Asp Tyr Leu
    50                  55                  60                  
Val Pro Glu Gln Gly Tyr Thr Lys Phe Ser Tyr Glu Phe Glu Thr Thr
65                  70                  75                  80  
Glu Lys Asp Ser Ser Asn Ile Glu Ile Thr Leu Ile Gly Ser Gly Thr
                85                  90                  95      
Thr Tyr Leu Asp Asn Leu Ser Ile Thr Glu Leu Asn Ser Thr Pro Glu
            100                 105                 110         
Ile Leu Asp Glu Pro Glu Val Lys Ile Pro Thr Asp Gln Glu Ile Met
        115                 120                 125             
Asp Ala His Lys Ile Tyr Phe Ala Asp Leu Asn Phe Asn Pro Ser Thr
    130                 135                 140                 
Gly Asn Thr Tyr Ile Asn Gly Met Tyr Phe Ala Pro Thr Gln Thr Asn
145                 150                 155                 160 
Lys Glu Ala Leu Asp Tyr Ile Gln Lys Tyr Arg Val Glu Ala Thr Leu
                165                 170                 175     
Gln Tyr Ser Gly Phe Lys Asp Ile Gly Thr Lys Asp Lys Glu Met Arg
            180                 185                 190         
Asn Tyr Leu Gly Asp Pro Asn Gln Pro Lys Thr Asn Tyr Val Asn Leu
        195                 200                 205             
Arg Ser Tyr Phe Thr Gly Gly Glu Asn Ile Met Thr Tyr Lys Lys Leu
    210                 215                 220                 
Arg Ile Tyr Ala Ile Thr Pro Asp Asp Arg Glu Leu Leu Val Leu Ser
225                 230                 235                 240 
Val Asp Gly Gly His His His His His His
                245                 250 


<210> 37
<211> 753
<212> DNA
<213> Artificial Sequence

<220> 
<223> nucleotide sequence of C55

<400> 37
atgaatacca ccaatcagga tggtctgcag ggtagcgcaa ataaactgaa tggtgaaacc 60
aaaatcaaaa ttccgatgag cgaactgaaa ccgtataaac gttatgtgtt tagcggctat 120
agcaaagatc cgctgaccag caatagcatt attgtgaaaa tcaaagccaa agaagaaaaa 180
accgattatc tggttccgga acagggttat accaaattta gctatgaatt tgaaaccacc 240
gaaaaagata gcagtaatat tgaaattacc ctgattggta gcggcaccac ctatctggat 300
aatctgagta ttaccgaact gaatagcaca ccggaaattc tggatgaacc ggaagtgaaa 360
attccgaccg atcaagaaat tatggatgcc cataaaatct attttgccga tctgaacttt 420
aatccgagca ccggcaatac ctatattaac ggcatgtatt ttgcaccgac ccagaccaat 480
aaagaagccc tggattatat tcagaaatat cgtgttgaag ccaccctgca gtatagcggt 540
tttaaagata ttggcaccaa agataaagaa atgcgtaatt atctgggcga tccgaatcag 600
ccgaaaacca attatgttaa tctgcgcagc tattttaccg gtggcgaaaa cattatgacc 660
tacaaaaaac tgcgcattta tgccattaca ccggatgatc gtgaactgct ggttctgagc 720
gttgatggcg gtcaccacca tcatcatcat taa                              753

<210> 38
<211> 170
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of CDTb prodomain sequence
      (long, aa43-211) (C58)

<400> 38
Met Glu Ile Val Asn Glu Asp Ile Leu Pro Asn Asn Gly Leu Met Gly
 1               5                  10                  15      
Tyr Tyr Phe Thr Asp Glu His Phe Lys Asp Leu Lys Leu Met Ala Pro
            20                  25                  30          
Ile Lys Asp Gly Asn Leu Lys Phe Glu Glu Lys Lys Val Asp Lys Leu
        35                  40                  45              
Leu Asp Lys Asp Lys Ser Asp Val Lys Ser Ile Arg Trp Thr Gly Arg
    50                  55                  60                  
Ile Ile Pro Ser Lys Asp Gly Glu Tyr Thr Leu Ser Thr Asp Arg Asp
65                  70                  75                  80  
Asp Val Leu Met Gln Val Asn Thr Glu Ser Thr Ile Ser Asn Thr Leu
                85                  90                  95      
Lys Val Asn Met Lys Lys Gly Lys Glu Tyr Lys Val Arg Ile Glu Leu
            100                 105                 110         
Gln Asp Lys Asn Leu Gly Ser Ile Asp Asn Leu Ser Ser Pro Asn Leu
        115                 120                 125             
Tyr Trp Glu Leu Asp Gly Met Lys Lys Ile Ile Pro Glu Glu Asn Leu
    130                 135                 140                 
Phe Leu Arg Asp Tyr Ser Asn Ile Glu Lys Asp Asp Pro Phe Ile Pro
145                 150                 155                 160 
Asn Asn Asn Phe Phe Asp Pro Lys Leu Met
                165                 170 


<210> 39
<211> 145
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of CDTb prodomain sequence
      (short, aa43-186) (C59)

<400> 39
Met Glu Ile Val Asn Glu Asp Ile Leu Pro Asn Asn Gly Leu Met Gly
 1               5                  10                  15      
Tyr Tyr Phe Thr Asp Glu His Phe Lys Asp Leu Lys Leu Met Ala Pro
            20                  25                  30          
Ile Lys Asp Gly Asn Leu Lys Phe Glu Glu Lys Lys Val Asp Lys Leu
        35                  40                  45              
Leu Asp Lys Asp Lys Ser Asp Val Lys Ser Ile Arg Trp Thr Gly Arg
    50                  55                  60                  
Ile Ile Pro Ser Lys Asp Gly Glu Tyr Thr Leu Ser Thr Asp Arg Asp
65                  70                  75                  80  
Asp Val Leu Met Gln Val Asn Thr Glu Ser Thr Ile Ser Asn Thr Leu
                85                  90                  95      
Lys Val Asn Met Lys Lys Gly Lys Glu Tyr Lys Val Arg Ile Glu Leu
            100                 105                 110         
Gln Asp Lys Asn Leu Gly Ser Ile Asp Asn Leu Ser Ser Pro Asn Leu
        115                 120                 125             
Tyr Trp Glu Leu Asp Gly Met Lys Lys Ile Ile Pro Glu Glu Asn Leu
    130                 135                 140                 
Phe
145 


<210> 40
<211> 489
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of Fusion CDTa N-term with
      linker (aa44-268) to CDTb receptor binding domain
      with linker in N term of sequence (aa621-876)
      (C60)

<400> 40
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Ser Leu Asp Phe Lys Asp Asp
    210                 215                 220                 
Thr Asn Phe Asp Asp Tyr Asn Asn Tyr Pro Ser Thr Trp Ser Asn Val
225                 230                 235                 240 
Asn Thr Thr Asn Gln Asp Gly Leu Gln Gly Ser Ala Asn Lys Leu Asn
                245                 250                 255     
Gly Glu Thr Lys Ile Lys Ile Pro Met Ser Glu Leu Lys Pro Tyr Lys
            260                 265                 270         
Arg Tyr Val Phe Ser Gly Tyr Ser Lys Asp Pro Leu Thr Ser Asn Ser
        275                 280                 285             
Ile Ile Val Lys Ile Lys Ala Lys Glu Glu Lys Thr Asp Tyr Leu Val
    290                 295                 300                 
Pro Glu Gln Gly Tyr Thr Lys Phe Ser Tyr Glu Phe Glu Thr Thr Glu
305                 310                 315                 320 
Lys Asp Ser Ser Asn Ile Glu Ile Thr Leu Ile Gly Ser Gly Thr Thr
                325                 330                 335     
Tyr Leu Asp Asn Leu Ser Ile Thr Glu Leu Asn Ser Thr Pro Glu Ile
            340                 345                 350         
Leu Asp Glu Pro Glu Val Lys Ile Pro Thr Asp Gln Glu Ile Met Asp
        355                 360                 365             
Ala His Lys Ile Tyr Phe Ala Asp Leu Asn Phe Asn Pro Ser Thr Gly
    370                 375                 380                 
Asn Thr Tyr Ile Asn Gly Met Tyr Phe Ala Pro Thr Gln Thr Asn Lys
385                 390                 395                 400 
Glu Ala Leu Asp Tyr Ile Gln Lys Tyr Arg Val Glu Ala Thr Leu Gln
                405                 410                 415     
Tyr Ser Gly Phe Lys Asp Ile Gly Thr Lys Asp Lys Glu Met Arg Asn
            420                 425                 430         
Tyr Leu Gly Asp Pro Asn Gln Pro Lys Thr Asn Tyr Val Asn Leu Arg
        435                 440                 445             
Ser Tyr Phe Thr Gly Gly Glu Asn Ile Met Thr Tyr Lys Lys Leu Arg
    450                 455                 460                 
Ile Tyr Ala Ile Thr Pro Asp Asp Arg Glu Leu Leu Val Leu Ser Val
465                 470                 475                 480 
Asp Gly Gly His His His His His His
                485                 


<210> 41
<211> 473
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of Fusion CDTa N-term with
      linker (aa44-268) to CDTb receptor binding domain
      without linker in N term of sequence (aa636-876)
      (C61)

<400> 41
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Ser Leu Asp Phe Lys Asp Asp
    210                 215                 220                 
Asn Thr Thr Asn Gln Asp Gly Leu Gln Gly Ser Ala Asn Lys Leu Asn
225                 230                 235                 240 
Gly Glu Thr Lys Ile Lys Ile Pro Met Ser Glu Leu Lys Pro Tyr Lys
                245                 250                 255     
Arg Tyr Val Phe Ser Gly Tyr Ser Lys Asp Pro Leu Thr Ser Asn Ser
            260                 265                 270         
Ile Ile Val Lys Ile Lys Ala Lys Glu Glu Lys Thr Asp Tyr Leu Val
        275                 280                 285             
Pro Glu Gln Gly Tyr Thr Lys Phe Ser Tyr Glu Phe Glu Thr Thr Glu
    290                 295                 300                 
Lys Asp Ser Ser Asn Ile Glu Ile Thr Leu Ile Gly Ser Gly Thr Thr
305                 310                 315                 320 
Tyr Leu Asp Asn Leu Ser Ile Thr Glu Leu Asn Ser Thr Pro Glu Ile
                325                 330                 335     
Leu Asp Glu Pro Glu Val Lys Ile Pro Thr Asp Gln Glu Ile Met Asp
            340                 345                 350         
Ala His Lys Ile Tyr Phe Ala Asp Leu Asn Phe Asn Pro Ser Thr Gly
        355                 360                 365             
Asn Thr Tyr Ile Asn Gly Met Tyr Phe Ala Pro Thr Gln Thr Asn Lys
    370                 375                 380                 
Glu Ala Leu Asp Tyr Ile Gln Lys Tyr Arg Val Glu Ala Thr Leu Gln
385                 390                 395                 400 
Tyr Ser Gly Phe Lys Asp Ile Gly Thr Lys Asp Lys Glu Met Arg Asn
                405                 410                 415     
Tyr Leu Gly Asp Pro Asn Gln Pro Lys Thr Asn Tyr Val Asn Leu Arg
            420                 425                 430         
Ser Tyr Phe Thr Gly Gly Glu Asn Ile Met Thr Tyr Lys Lys Leu Arg
        435                 440                 445             
Ile Tyr Ala Ile Thr Pro Asp Asp Arg Glu Leu Leu Val Leu Ser Val
    450                 455                 460                 
Asp Gly Gly His His His His His His
465                 470             


<210> 42
<211> 482
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of Fusion CDTa N-term without
      linker (aa44-260) to CDTb receptor binding domain
      with linker in N term of sequence (aa621-876)
      (C62)

<400> 42
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Thr Asn Phe Asp Asp Tyr Asn
    210                 215                 220                 
Asn Tyr Pro Ser Thr Trp Ser Asn Val Asn Thr Thr Asn Gln Asp Gly
225                 230                 235                 240 
Leu Gln Gly Ser Ala Asn Lys Leu Asn Gly Glu Thr Lys Ile Lys Ile
                245                 250                 255     
Pro Met Ser Glu Leu Lys Pro Tyr Lys Arg Tyr Val Phe Ser Gly Tyr
            260                 265                 270         
Ser Lys Asp Pro Leu Thr Ser Asn Ser Ile Ile Val Lys Ile Lys Ala
        275                 280                 285             
Lys Glu Glu Lys Thr Asp Tyr Leu Val Pro Glu Gln Gly Tyr Thr Lys
    290                 295                 300                 
Phe Ser Tyr Glu Phe Glu Thr Thr Glu Lys Asp Ser Ser Asn Ile Glu
305                 310                 315                 320 
Ile Thr Leu Ile Gly Ser Gly Thr Thr Tyr Leu Asp Asn Leu Ser Ile
                325                 330                 335     
Thr Glu Leu Asn Ser Thr Pro Glu Ile Leu Asp Glu Pro Glu Val Lys
            340                 345                 350         
Ile Pro Thr Asp Gln Glu Ile Met Asp Ala His Lys Ile Tyr Phe Ala
        355                 360                 365             
Asp Leu Asn Phe Asn Pro Ser Thr Gly Asn Thr Tyr Ile Asn Gly Met
    370                 375                 380                 
Tyr Phe Ala Pro Thr Gln Thr Asn Lys Glu Ala Leu Asp Tyr Ile Gln
385                 390                 395                 400 
Lys Tyr Arg Val Glu Ala Thr Leu Gln Tyr Ser Gly Phe Lys Asp Ile
                405                 410                 415     
Gly Thr Lys Asp Lys Glu Met Arg Asn Tyr Leu Gly Asp Pro Asn Gln
            420                 425                 430         
Pro Lys Thr Asn Tyr Val Asn Leu Arg Ser Tyr Phe Thr Gly Gly Glu
        435                 440                 445             
Asn Ile Met Thr Tyr Lys Lys Leu Arg Ile Tyr Ala Ile Thr Pro Asp
    450                 455                 460                 
Asp Arg Glu Leu Leu Val Leu Ser Val Asp Gly Gly His His His His
465                 470                 475                 480 
His His
        


<210> 43
<211> 466
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of Fusion CDTa N-term without
      linker (aa44-260) to CDTb receptor binding domain
      without linker in N term of sequence (aa636-876)
      (C63)

<400> 43
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Asn Thr Thr Asn Gln Asp Gly
    210                 215                 220                 
Leu Gln Gly Ser Ala Asn Lys Leu Asn Gly Glu Thr Lys Ile Lys Ile
225                 230                 235                 240 
Pro Met Ser Glu Leu Lys Pro Tyr Lys Arg Tyr Val Phe Ser Gly Tyr
                245                 250                 255     
Ser Lys Asp Pro Leu Thr Ser Asn Ser Ile Ile Val Lys Ile Lys Ala
            260                 265                 270         
Lys Glu Glu Lys Thr Asp Tyr Leu Val Pro Glu Gln Gly Tyr Thr Lys
        275                 280                 285             
Phe Ser Tyr Glu Phe Glu Thr Thr Glu Lys Asp Ser Ser Asn Ile Glu
    290                 295                 300                 
Ile Thr Leu Ile Gly Ser Gly Thr Thr Tyr Leu Asp Asn Leu Ser Ile
305                 310                 315                 320 
Thr Glu Leu Asn Ser Thr Pro Glu Ile Leu Asp Glu Pro Glu Val Lys
                325                 330                 335     
Ile Pro Thr Asp Gln Glu Ile Met Asp Ala His Lys Ile Tyr Phe Ala
            340                 345                 350         
Asp Leu Asn Phe Asn Pro Ser Thr Gly Asn Thr Tyr Ile Asn Gly Met
        355                 360                 365             
Tyr Phe Ala Pro Thr Gln Thr Asn Lys Glu Ala Leu Asp Tyr Ile Gln
    370                 375                 380                 
Lys Tyr Arg Val Glu Ala Thr Leu Gln Tyr Ser Gly Phe Lys Asp Ile
385                 390                 395                 400 
Gly Thr Lys Asp Lys Glu Met Arg Asn Tyr Leu Gly Asp Pro Asn Gln
                405                 410                 415     
Pro Lys Thr Asn Tyr Val Asn Leu Arg Ser Tyr Phe Thr Gly Gly Glu
            420                 425                 430         
Asn Ile Met Thr Tyr Lys Lys Leu Arg Ile Tyr Ala Ile Thr Pro Asp
        435                 440                 445             
Asp Arg Glu Leu Leu Val Leu Ser Val Asp Gly Gly His His His His
    450                 455                 460                 
His His
465     


<210> 44
<211> 1231
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of Fusion F2- CDTb receptor
      binding domain with linker in N term of sequence
      (aa621-876) (C64)

<400> 44
Met Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn
 1               5                  10                  15      
Thr Ala Ile Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe
            20                  25                  30          
Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro
        35                  40                  45              
Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile
    50                  55                  60                  
Glu Gly Gln Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly
65                  70                  75                  80  
Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg
                85                  90                  95      
Ile Ile Asn Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala
            100                 105                 110         
Ala Ile His Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr
        115                 120                 125             
Asp Gly Ile Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe
    130                 135                 140                 
Tyr Phe Asp Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys
145                 150                 155                 160 
Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn
                165                 170                 175     
Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu
            180                 185                 190         
Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly
        195                 200                 205             
Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala
    210                 215                 220                 
Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe
225                 230                 235                 240 
Asn Leu Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly
                245                 250                 255     
Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr
            260                 265                 270         
Thr Ser Ile Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met
        275                 280                 285             
Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro
    290                 295                 300                 
Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln
305                 310                 315                 320 
Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp
                325                 330                 335     
Ser Lys Ala Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr
            340                 345                 350         
Phe Asn Thr Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn
        355                 360                 365             
Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly
    370                 375                 380                 
Tyr Thr Ile Ile Ser Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile
385                 390                 395                 400 
Met Gln Ile Gly Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr Phe Ala
                405                 410                 415     
Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr
            420                 425                 430         
Gln Asn Arg Phe Leu Tyr Leu His Asp Asn Ile Tyr Tyr Phe Gly Asn
        435                 440                 445             
Asn Ser Lys Ala Ala Thr Gly Trp Val Thr Ile Asp Gly Asn Arg Tyr
    450                 455                 460                 
Tyr Phe Glu Pro Asn Thr Ala Met Gly Ala Asn Gly Tyr Lys Thr Ile
465                 470                 475                 480 
Asp Asn Lys Asn Phe Tyr Phe Arg Asn Gly Leu Pro Gln Ile Gly Val
                485                 490                 495     
Phe Lys Gly Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp
            500                 505                 510         
Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg Phe Leu
        515                 520                 525             
His Leu Leu Gly Lys Ile Tyr Tyr Phe Gly Asn Asn Ser Lys Ala Val
    530                 535                 540                 
Thr Gly Trp Gln Thr Ile Asn Gly Lys Val Tyr Tyr Phe Met Pro Asp
545                 550                 555                 560 
Thr Ala Met Ala Ala Ala Gly Gly Leu Asn Gln Ile Gly Asp Tyr Lys
                565                 570                 575     
Tyr Tyr Phe Asn Ser Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile
            580                 585                 590         
Asn Asp Asn Lys His Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly
        595                 600                 605             
Tyr Thr Glu Ile Asp Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu
    610                 615                 620                 
Met Gln Ile Gly Val Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala
625                 630                 635                 640 
His His Asn Glu Asp Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr
                645                 650                 655     
Ser Gly Ile Leu Asn Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser
            660                 665                 670         
Phe Thr Ala Val Val Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr
        675                 680                 685             
Tyr Phe Asp Glu Asp Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile
    690                 695                 700                 
Asn Asp Gly Gln Tyr Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly
705                 710                 715                 720 
Phe Val Thr Ile Asn Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile
                725                 730                 735     
Ile Glu Ser Gly Val Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp
            740                 745                 750         
Asp Asn Gly Ile Val Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr
        755                 760                 765             
Lys Tyr Phe Ala Pro Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln
    770                 775                 780                 
Ala Val Glu Tyr Ser Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr
785                 790                 795                 800 
Phe Gly Glu Thr Tyr Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu
                805                 810                 815     
Asn Glu Ser Asp Lys Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys
            820                 825                 830         
Lys Gly Ile Asn Leu Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys
        835                 840                 845             
Gly Ile Met Arg Thr Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr
    850                 855                 860                 
Phe Asn Glu Asn Gly Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp
865                 870                 875                 880 
Lys Met Phe Tyr Phe Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe
                885                 890                 895     
Asn Thr Pro Asp Gly Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp
            900                 905                 910         
Glu Asn Phe Glu Gly Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu
        915                 920                 925             
Asp Glu Lys Arg Tyr Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly
    930                 935                 940                 
Ser Val Ile Ile Asp Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala
945                 950                 955                 960 
Gln Leu Val Ile Ser Glu Thr Asn Phe Asp Asp Tyr Asn Asn Tyr Pro
                965                 970                 975     
Ser Thr Trp Ser Asn Val Asn Thr Thr Asn Gln Asp Gly Leu Gln Gly
            980                 985                 990         
Ser Ala Asn Lys Leu Asn Gly Glu Thr Lys Ile Lys Ile Pro Met Ser
        995                 1000                1005            
Glu Leu Lys Pro Tyr Lys Arg Tyr Val Phe Ser Gly Tyr Ser Lys Asp
    1010                1015                1020                
Pro Leu Thr Ser Asn Ser Ile Ile Val Lys Ile Lys Ala Lys Glu Glu
1025                1030                1035                1040
Lys Thr Asp Tyr Leu Val Pro Glu Gln Gly Tyr Thr Lys Phe Ser Tyr
                1045                1050                1055    
Glu Phe Glu Thr Thr Glu Lys Asp Ser Ser Asn Ile Glu Ile Thr Leu
            1060                1065                1070        
Ile Gly Ser Gly Thr Thr Tyr Leu Asp Asn Leu Ser Ile Thr Glu Leu
        1075                1080                1085            
Asn Ser Thr Pro Glu Ile Leu Asp Glu Pro Glu Val Lys Ile Pro Thr
    1090                1095                1100                
Asp Gln Glu Ile Met Asp Ala His Lys Ile Tyr Phe Ala Asp Leu Asn
1105                1110                1115                1120
Phe Asn Pro Ser Thr Gly Asn Thr Tyr Ile Asn Gly Met Tyr Phe Ala
                1125                1130                1135    
Pro Thr Gln Thr Asn Lys Glu Ala Leu Asp Tyr Ile Gln Lys Tyr Arg
            1140                1145                1150        
Val Glu Ala Thr Leu Gln Tyr Ser Gly Phe Lys Asp Ile Gly Thr Lys
        1155                1160                1165            
Asp Lys Glu Met Arg Asn Tyr Leu Gly Asp Pro Asn Gln Pro Lys Thr
    1170                1175                1180                
Asn Tyr Val Asn Leu Arg Ser Tyr Phe Thr Gly Gly Glu Asn Ile Met
1185                1190                1195                1200
Thr Tyr Lys Lys Leu Arg Ile Tyr Ala Ile Thr Pro Asp Asp Arg Glu
                1205                1210                1215    
Leu Leu Val Leu Ser Val Asp Gly Gly His His His His His His
            1220                1225                1230    


<210> 45
<211> 1219
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of Fusion of F2 to CDTb
      receptor binding domain without linker in N term
      of sequence (aa636-876) with 2 heterogeneous Gly
      residues between F2 and CTDb sequences (C65)

<400> 45
Met Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Thr Asn
 1               5                  10                  15      
Thr Ala Ile Ala Ser Thr Gly Tyr Thr Ile Ile Asn Gly Lys His Phe
            20                  25                  30          
Tyr Phe Asn Thr Asp Gly Ile Met Gln Ile Gly Val Phe Lys Gly Pro
        35                  40                  45              
Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp Ala Asn Asn Ile
    50                  55                  60                  
Glu Gly Gln Ala Ile Leu Tyr Gln Asn Glu Phe Leu Thr Leu Asn Gly
65                  70                  75                  80  
Lys Lys Tyr Tyr Phe Gly Ser Asp Ser Lys Ala Val Thr Gly Trp Arg
                85                  90                  95      
Ile Ile Asn Asn Lys Lys Tyr Tyr Phe Asn Pro Asn Asn Ala Ile Ala
            100                 105                 110         
Ala Ile His Leu Cys Thr Ile Asn Asn Asp Lys Tyr Tyr Phe Ser Tyr
        115                 120                 125             
Asp Gly Ile Leu Gln Asn Gly Tyr Ile Thr Ile Glu Arg Asn Asn Phe
    130                 135                 140                 
Tyr Phe Asp Ala Asn Asn Glu Ser Lys Met Val Thr Gly Val Phe Lys
145                 150                 155                 160 
Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr His Asn Asn
                165                 170                 175     
Asn Ile Glu Gly Gln Ala Ile Val Tyr Gln Asn Lys Phe Leu Thr Leu
            180                 185                 190         
Asn Gly Lys Lys Tyr Tyr Phe Asp Asn Asp Ser Lys Ala Val Thr Gly
        195                 200                 205             
Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe Asn Leu Asn Thr Ala
    210                 215                 220                 
Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly Lys Lys Tyr Tyr Phe
225                 230                 235                 240 
Asn Leu Asn Thr Ala Glu Ala Ala Thr Gly Trp Gln Thr Ile Asp Gly
                245                 250                 255     
Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Phe Ile Ala Ser Thr Gly Tyr
            260                 265                 270         
Thr Ser Ile Asn Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile Met
        275                 280                 285             
Gln Ile Gly Val Phe Lys Gly Pro Asn Gly Phe Glu Tyr Phe Ala Pro
    290                 295                 300                 
Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Leu Tyr Gln
305                 310                 315                 320 
Asn Lys Phe Leu Thr Leu Asn Gly Lys Lys Tyr Tyr Phe Gly Ser Asp
                325                 330                 335     
Ser Lys Ala Val Thr Gly Leu Arg Thr Ile Asp Gly Lys Lys Tyr Tyr
            340                 345                 350         
Phe Asn Thr Asn Thr Ala Val Ala Val Thr Gly Trp Gln Thr Ile Asn
        355                 360                 365             
Gly Lys Lys Tyr Tyr Phe Asn Thr Asn Thr Ser Ile Ala Ser Thr Gly
    370                 375                 380                 
Tyr Thr Ile Ile Ser Gly Lys His Phe Tyr Phe Asn Thr Asp Gly Ile
385                 390                 395                 400 
Met Gln Ile Gly Val Phe Lys Gly Pro Asp Gly Phe Glu Tyr Phe Ala
                405                 410                 415     
Pro Ala Asn Thr Asp Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr
            420                 425                 430         
Gln Asn Arg Phe Leu Tyr Leu His Asp Asn Ile Tyr Tyr Phe Gly Asn
        435                 440                 445             
Asn Ser Lys Ala Ala Thr Gly Trp Val Thr Ile Asp Gly Asn Arg Tyr
    450                 455                 460                 
Tyr Phe Glu Pro Asn Thr Ala Met Gly Ala Asn Gly Tyr Lys Thr Ile
465                 470                 475                 480 
Asp Asn Lys Asn Phe Tyr Phe Arg Asn Gly Leu Pro Gln Ile Gly Val
                485                 490                 495     
Phe Lys Gly Ser Asn Gly Phe Glu Tyr Phe Ala Pro Ala Asn Thr Asp
            500                 505                 510         
Ala Asn Asn Ile Glu Gly Gln Ala Ile Arg Tyr Gln Asn Arg Phe Leu
        515                 520                 525             
His Leu Leu Gly Lys Ile Tyr Tyr Phe Gly Asn Asn Ser Lys Ala Val
    530                 535                 540                 
Thr Gly Trp Gln Thr Ile Asn Gly Lys Val Tyr Tyr Phe Met Pro Asp
545                 550                 555                 560 
Thr Ala Met Ala Ala Ala Gly Gly Leu Asn Gln Ile Gly Asp Tyr Lys
                565                 570                 575     
Tyr Tyr Phe Asn Ser Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile
            580                 585                 590         
Asn Asp Asn Lys His Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly
        595                 600                 605             
Tyr Thr Glu Ile Asp Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu
    610                 615                 620                 
Met Gln Ile Gly Val Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala
625                 630                 635                 640 
His His Asn Glu Asp Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr
                645                 650                 655     
Ser Gly Ile Leu Asn Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser
            660                 665                 670         
Phe Thr Ala Val Val Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr
        675                 680                 685             
Tyr Phe Asp Glu Asp Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile
    690                 695                 700                 
Asn Asp Gly Gln Tyr Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly
705                 710                 715                 720 
Phe Val Thr Ile Asn Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile
                725                 730                 735     
Ile Glu Ser Gly Val Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp
            740                 745                 750         
Asp Asn Gly Ile Val Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr
        755                 760                 765             
Lys Tyr Phe Ala Pro Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln
    770                 775                 780                 
Ala Val Glu Tyr Ser Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr
785                 790                 795                 800 
Phe Gly Glu Thr Tyr Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu
                805                 810                 815     
Asn Glu Ser Asp Lys Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys
            820                 825                 830         
Lys Gly Ile Asn Leu Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys
        835                 840                 845             
Gly Ile Met Arg Thr Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr
    850                 855                 860                 
Phe Asn Glu Asn Gly Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp
865                 870                 875                 880 
Lys Met Phe Tyr Phe Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe
                885                 890                 895     
Asn Thr Pro Asp Gly Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp
            900                 905                 910         
Glu Asn Phe Glu Gly Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu
        915                 920                 925             
Asp Glu Lys Arg Tyr Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly
    930                 935                 940                 
Ser Val Ile Ile Asp Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala
945                 950                 955                 960 
Gln Leu Val Ile Ser Glu Gly Gly Asn Val Asn Thr Thr Asn Gln Asp
                965                 970                 975     
Gly Leu Gln Gly Ser Ala Asn Lys Leu Asn Gly Glu Thr Lys Ile Lys
            980                 985                 990         
Ile Pro Met Ser Glu Leu Lys Pro Tyr Lys Arg Tyr Val Phe Ser Gly
        995                 1000                1005            
Tyr Ser Lys Asp Pro Leu Thr Ser Asn Ser Ile Ile Val Lys Ile Lys
    1010                1015                1020                
Ala Lys Glu Glu Lys Thr Asp Tyr Leu Val Pro Glu Gln Gly Tyr Thr
1025                1030                1035                1040
Lys Phe Ser Tyr Glu Phe Glu Thr Thr Glu Lys Asp Ser Ser Asn Ile
                1045                1050                1055    
Glu Ile Thr Leu Ile Gly Ser Gly Thr Thr Tyr Leu Asp Asn Leu Ser
            1060                1065                1070        
Ile Thr Glu Leu Asn Ser Thr Pro Glu Ile Leu Asp Glu Pro Glu Val
        1075                1080                1085            
Lys Ile Pro Thr Asp Gln Glu Ile Met Asp Ala His Lys Ile Tyr Phe
    1090                1095                1100                
Ala Asp Leu Asn Phe Asn Pro Ser Thr Gly Asn Thr Tyr Ile Asn Gly
1105                1110                1115                1120
Met Tyr Phe Ala Pro Thr Gln Thr Asn Lys Glu Ala Leu Asp Tyr Ile
                1125                1130                1135    
Gln Lys Tyr Arg Val Glu Ala Thr Leu Gln Tyr Ser Gly Phe Lys Asp
            1140                1145                1150        
Ile Gly Thr Lys Asp Lys Glu Met Arg Asn Tyr Leu Gly Asp Pro Asn
        1155                1160                1165            
Gln Pro Lys Thr Asn Tyr Val Asn Leu Arg Ser Tyr Phe Thr Gly Gly
    1170                1175                1180                
Glu Asn Ile Met Thr Tyr Lys Lys Leu Arg Ile Tyr Ala Ile Thr Pro
1185                1190                1195                1200
Asp Asp Arg Glu Leu Leu Val Leu Ser Val Asp Gly Gly His His His
                1205                1210                1215    
His His His
            


<210> 46
<211> 419
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of CDTa without signal
      peptide, with two mutations (E428Q, E430Q, aa
      44-463) (C67)

<400> 46
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Ser Leu Asp Phe Lys Asp Asp
    210                 215                 220                 
Val Ser Lys Gly Asp Ser Trp Gly Lys Ala Asn Tyr Asn Asp Trp Ser
225                 230                 235                 240 
Asn Lys Leu Thr Pro Asn Glu Leu Ala Asp Val Asn Asp Tyr Met Arg
                245                 250                 255     
Gly Gly Tyr Thr Ala Ile Asn Asn Tyr Leu Ile Ser Asn Gly Pro Val
            260                 265                 270         
Asn Asn Pro Asn Pro Glu Leu Asp Ser Lys Ile Thr Asn Ile Glu Asn
        275                 280                 285             
Ala Leu Lys Arg Glu Pro Ile Pro Thr Asn Leu Thr Val Tyr Arg Arg
    290                 295                 300                 
Ser Gly Pro Gln Glu Phe Gly Leu Thr Leu Thr Ser Pro Glu Tyr Asp
305                 310                 315                 320 
Phe Asn Lys Leu Glu Asn Ile Asp Ala Phe Lys Ser Lys Trp Glu Gly
                325                 330                 335     
Gln Ala Leu Ser Tyr Pro Asn Phe Ile Ser Thr Ser Ile Gly Ser Val
            340                 345                 350         
Asn Met Ser Ala Phe Ala Lys Arg Lys Ile Val Leu Arg Ile Thr Ile
        355                 360                 365             
Pro Lys Gly Ser Pro Gly Ala Tyr Leu Ser Ala Ile Pro Gly Tyr Ala
    370                 375                 380                 
Gly Tyr Val Leu Leu Asn His Gly Ser Lys Phe Lys Ile Asn Lys Ile
385                 390                 395                 400 
Asp Ser Tyr Lys Asp Gly Thr Ile Thr Lys Leu Ile Val Asp Ala Thr
                405                 410                 415     
Leu Ile Pro
            


<210> 47
<211> 1266
<212> DNA
<213> Artificial Sequence

<220> 
<223> nucleotide sequence of C67

<400> 47
atggtttgca ataccaccta taaagcaccg attgaacgtc cggaagattt tctgaaagat 60
aaagaaaaag ccaaagaatg ggaacgcaaa gaagcagaac gtattgaaca gaaactggaa 120
cgtagcgaaa aagaagcact ggaaagctac aaaaaagata gcgtggaaat ttcaaaatat 180
agccagaccc gcaattattt ctatgattat cagattgaag ccaatagccg tgaaaaagaa 240
tataaagaac tgcgcaatgc cattagcaaa aacaaaattg ataaaccgat gtatgtgtat 300
tatttcgaaa gtccggaaaa atttgccttt aacaaagtga ttcgcaccga aaatcagaat 360
gaaattagcc tggaaaaatt caatgaattt aaagaaacca ttcagaataa actgtttaaa 420
caggatggct ttaaagatat ttcactgtat gaaccgggta aaggtgatga aaaaccgaca 480
ccgctgctga tgcatctgaa actgcctcgt aataccggta tgctgccgta taccaatacc 540
aataatgtta gcaccctgat tgaacagggc tatagcatca aaattgataa aattgtgcgc 600
attgtgattg atggcaaaca ttatatcaaa gccgaagcca gcgttgtttc aagcctggat 660
tttaaagatg atgtgagcaa aggcgatagc tggggtaaag caaactataa tgattggagc 720
aataaactga ccccgaatga actggcagat gtgaatgatt atatgcgtgg tggttatacc 780
gccattaaca attatctgat tagcaatggt ccggtgaata atccgaatcc ggaactggat 840
agcaaaatta ccaatattga aaatgccctg aaacgcgaac cgattccgac caatctgacc 900
gtttatcgtc gtagcggtcc gcaagaattt ggtctgaccc tgaccagtcc ggaatatgac 960
tttaacaaac tggaaaatat tgatgccttt aaaagcaaat gggaaggtca ggcactgagc 1020
tatccgaact ttattagcac cagcattggt agcgttaata tgagcgcatt tgccaaacgt 1080
aaaattgtgc tgcgtattac cattccgaaa ggtagtccgg gtgcatatct gagcgcaatt 1140
ccgggttatg ccggtcaata tcaggttctg ctgaatcatg gcagcaaatt caaaattaac 1200
aaaattgata gctataaaga tggcaccatt accaaactga ttgttgatgc aaccctgatt 1260
ccgtaa                                                            1266

<210> 48
<211> 421
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of CDTa without signal
      peptide, with seven mutations (R345A, Q350A,
      N385A, R402A, S388F, E428Q, E430Q, aa 44-463)
      (C69)

<400> 48
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Ser Leu Asp Phe Lys Asp Asp
    210                 215                 220                 
Val Ser Lys Gly Asp Ser Trp Gly Lys Ala Asn Tyr Asn Asp Trp Ser
225                 230                 235                 240 
Asn Lys Leu Thr Pro Asn Glu Leu Ala Asp Val Asn Asp Tyr Met Arg
                245                 250                 255     
Gly Gly Tyr Thr Ala Ile Asn Asn Tyr Leu Ile Ser Asn Gly Pro Val
            260                 265                 270         
Asn Asn Pro Asn Pro Glu Leu Asp Ser Lys Ile Thr Asn Ile Glu Asn
        275                 280                 285             
Ala Leu Lys Arg Glu Pro Ile Pro Thr Asn Leu Thr Val Tyr Ala Arg
    290                 295                 300                 
Ser Gly Pro Ala Glu Phe Gly Leu Thr Leu Thr Ser Pro Glu Tyr Asp
305                 310                 315                 320 
Phe Asn Lys Leu Glu Asn Ile Asp Ala Phe Lys Ser Lys Trp Glu Gly
                325                 330                 335     
Gln Ala Leu Ser Tyr Pro Ala Phe Ile Phe Thr Ser Ile Gly Ser Val
            340                 345                 350         
Asn Met Ser Ala Phe Ala Lys Ala Lys Ile Val Leu Arg Ile Thr Ile
        355                 360                 365             
Pro Lys Gly Ser Pro Gly Ala Tyr Leu Ser Ala Ile Pro Gly Tyr Ala
    370                 375                 380                 
Gly Gln Tyr Gln Val Leu Leu Asn His Gly Ser Lys Phe Lys Ile Asn
385                 390                 395                 400 
Lys Ile Asp Ser Tyr Lys Asp Gly Thr Ile Thr Lys Leu Ile Val Asp
                405                 410                 415     
Ala Thr Leu Ile Pro
            420     


<210> 49
<211> 1263
<212> DNA
<213> Artificial Sequence

<220> 
<223> nucleotide sequence of C69

<400> 49
atggtttgca ataccaccta taaagcaccg attgaacgtc cggaagattt tctgaaagat 60
aaagaaaaag ccaaagaatg ggaacgcaaa gaagcagaac gtattgaaca gaaactggaa 120
cgtagcgaaa aagaagcact ggaaagctac aaaaaagata gcgtggaaat ttcaaaatat 180
agccagaccc gcaattattt ctatgattat cagattgaag ccaatagccg tgaaaaagaa 240
tataaagaac tgcgcaatgc cattagcaaa aacaaaattg ataaaccgat gtatgtgtat 300
tatttcgaaa gtccggaaaa atttgccttt aacaaagtga ttcgcaccga aaatcagaat 360
gaaattagcc tggaaaaatt caatgaattt aaagaaacca ttcagaataa actgtttaaa 420
caggatggct ttaaagatat ttcactgtat gaaccgggta aaggtgatga aaaaccgaca 480
ccgctgctga tgcatctgaa actgcctcgt aataccggta tgctgccgta taccaatacc 540
aataatgtta gcaccctgat tgaacagggc tatagcatca aaattgataa aattgtgcgc 600
attgtgattg atggcaaaca ttatatcaaa gccgaagcca gcgttgtttc aagcctggat 660
tttaaagatg atgtgagcaa aggcgatagc tggggtaaag caaactataa tgattggagc 720
aataaactga ccccgaatga actggcagat gtgaatgatt atatgcgtgg tggttatacc 780
gccattaaca attatctgat tagcaatggt ccggtgaata atccgaatcc ggaactggat 840
agcaaaatta ccaatattga aaatgccctg aaacgcgaac cgattccgac caatctgacc 900
gtttatgcac gtagcggtcc ggcagaattt ggtctgaccc tgaccagtcc ggaatatgac 960
tttaacaaac tggaaaatat tgatgccttt aaaagcaaat gggaaggtca ggcactgagc 1020
tatccggcat ttattttcac cagcattggt agcgttaata tgagcgcatt tgccaaagca 1080
aaaattgtgc tgcgtattac cattccgaaa ggtagtccgg gtgcatatct gagcgcaatt 1140
ccgggttatg ccggtcagta tcaggttctg ctgaatcatg gcagcaaatt caaaattaac 1200
aaaattgata gctataaaga tggcaccatt accaaactga ttgttgatgc aaccctgatt 1260
ccg                                                               1263

<210> 50
<211> 672
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of CDTb without signal
      sequence and prodomain (mature fragment based on
      MS data) with Ca2+ binding motif mutation
      (aa212-876, mut Asp-9-11-13 Ala) (C97)

<400> 50
Met Ser Asp Trp Glu Asp Glu Asp Leu Ala Thr Ala Asn Ala Asn Ile
 1               5                  10                  15      
Pro Asp Ser Tyr Glu Arg Asn Gly Tyr Thr Ile Lys Asp Leu Ile Ala
            20                  25                  30          
Val Lys Trp Glu Asp Ser Phe Ala Glu Gln Gly Tyr Lys Lys Tyr Val
        35                  40                  45              
Ser Asn Tyr Leu Glu Ser Asn Thr Ala Gly Asp Pro Tyr Thr Asp Tyr
    50                  55                  60                  
Glu Lys Ala Ser Gly Ser Phe Asp Lys Ala Ile Lys Thr Glu Ala Arg
65                  70                  75                  80  
Asp Pro Leu Val Ala Ala Tyr Pro Ile Val Gly Val Gly Met Glu Lys
                85                  90                  95      
Leu Ile Ile Ser Thr Asn Glu His Ala Ser Thr Asp Gln Gly Lys Thr
            100                 105                 110         
Val Ser Arg Ala Thr Thr Asn Ser Lys Thr Glu Ser Asn Thr Ala Gly
        115                 120                 125             
Val Ser Val Asn Val Gly Tyr Gln Asn Gly Phe Thr Ala Asn Val Thr
    130                 135                 140                 
Thr Asn Tyr Ser His Thr Thr Asp Asn Ser Thr Ala Val Gln Asp Ser
145                 150                 155                 160 
Asn Gly Glu Ser Trp Asn Thr Gly Leu Ser Ile Asn Lys Gly Glu Ser
                165                 170                 175     
Ala Tyr Ile Asn Ala Asn Val Arg Tyr Tyr Asn Thr Gly Thr Ala Pro
            180                 185                 190         
Met Tyr Lys Val Thr Pro Thr Thr Asn Leu Val Leu Asp Gly Asp Thr
        195                 200                 205             
Leu Ser Thr Ile Lys Ala Gln Glu Asn Gln Ile Gly Asn Asn Leu Ser
    210                 215                 220                 
Pro Gly Asp Thr Tyr Pro Lys Lys Gly Leu Ser Pro Leu Ala Leu Asn
225                 230                 235                 240 
Thr Met Asp Gln Phe Ser Ser Arg Leu Ile Pro Ile Asn Tyr Asp Gln
                245                 250                 255     
Leu Lys Lys Leu Asp Ala Gly Lys Gln Ile Lys Leu Glu Thr Thr Gln
            260                 265                 270         
Val Ser Gly Asn Phe Gly Thr Lys Asn Ser Ser Gly Gln Ile Val Thr
        275                 280                 285             
Glu Gly Asn Ser Trp Ser Asp Tyr Ile Ser Gln Ile Asp Ser Ile Ser
    290                 295                 300                 
Ala Ser Ile Ile Leu Asp Thr Glu Asn Glu Ser Tyr Glu Arg Arg Val
305                 310                 315                 320 
Thr Ala Lys Asn Leu Gln Asp Pro Glu Asp Lys Thr Pro Glu Leu Thr
                325                 330                 335     
Ile Gly Glu Ala Ile Glu Lys Ala Phe Gly Ala Thr Lys Lys Asp Gly
            340                 345                 350         
Leu Leu Tyr Phe Asn Asp Ile Pro Ile Asp Glu Ser Cys Val Glu Leu
        355                 360                 365             
Ile Phe Asp Asp Asn Thr Ala Asn Lys Ile Lys Asp Ser Leu Lys Thr
    370                 375                 380                 
Leu Ser Asp Lys Lys Ile Tyr Asn Val Lys Leu Glu Arg Gly Met Asn
385                 390                 395                 400 
Ile Leu Ile Lys Thr Pro Thr Tyr Phe Thr Asn Phe Asp Asp Tyr Asn
                405                 410                 415     
Asn Tyr Pro Ser Thr Trp Ser Asn Val Asn Thr Thr Asn Gln Asp Gly
            420                 425                 430         
Leu Gln Gly Ser Ala Asn Lys Leu Asn Gly Glu Thr Lys Ile Lys Ile
        435                 440                 445             
Pro Met Ser Glu Leu Lys Pro Tyr Lys Arg Tyr Val Phe Ser Gly Tyr
    450                 455                 460                 
Ser Lys Asp Pro Leu Thr Ser Asn Ser Ile Ile Val Lys Ile Lys Ala
465                 470                 475                 480 
Lys Glu Glu Lys Thr Asp Tyr Leu Val Pro Glu Gln Gly Tyr Thr Lys
                485                 490                 495     
Phe Ser Tyr Glu Phe Glu Thr Thr Glu Lys Asp Ser Ser Asn Ile Glu
            500                 505                 510         
Ile Thr Leu Ile Gly Ser Gly Thr Thr Tyr Leu Asp Asn Leu Ser Ile
        515                 520                 525             
Thr Glu Leu Asn Ser Thr Pro Glu Ile Leu Asp Glu Pro Glu Val Lys
    530                 535                 540                 
Ile Pro Thr Asp Gln Glu Ile Met Asp Ala His Lys Ile Tyr Phe Ala
545                 550                 555                 560 
Asp Leu Asn Phe Asn Pro Ser Thr Gly Asn Thr Tyr Ile Asn Gly Met
                565                 570                 575     
Tyr Phe Ala Pro Thr Gln Thr Asn Lys Glu Ala Leu Asp Tyr Ile Gln
            580                 585                 590         
Lys Tyr Arg Val Glu Ala Thr Leu Gln Tyr Ser Gly Phe Lys Asp Ile
        595                 600                 605             
Gly Thr Lys Asp Lys Glu Met Arg Asn Tyr Leu Gly Asp Pro Asn Gln
    610                 615                 620                 
Pro Lys Thr Asn Tyr Val Asn Leu Arg Ser Tyr Phe Thr Gly Gly Glu
625                 630                 635                 640 
Asn Ile Met Thr Tyr Lys Lys Leu Arg Ile Tyr Ala Ile Thr Pro Asp
                645                 650                 655     
Asp Arg Glu Leu Leu Val Leu Ser Val Asp His His His His His His
            660                 665                 670         


<210> 51
<211> 672
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of CDTb with prodomain removed
      (CDTb'', aa212-876) (C55)

<400> 51
Met Ser Asp Trp Glu Asp Glu Asp Leu Asp Thr Asp Asn Asp Asn Ile
 1               5                  10                  15      
Pro Asp Ser Tyr Glu Arg Asn Gly Tyr Thr Ile Lys Asp Leu Ile Ala
            20                  25                  30          
Val Lys Trp Glu Asp Ser Phe Ala Glu Gln Gly Tyr Lys Lys Tyr Val
        35                  40                  45              
Ser Asn Tyr Leu Glu Ser Asn Thr Ala Gly Asp Pro Tyr Thr Asp Tyr
    50                  55                  60                  
Glu Lys Ala Ser Gly Ser Phe Asp Lys Ala Ile Lys Thr Glu Ala Arg
65                  70                  75                  80  
Asp Pro Leu Val Ala Ala Tyr Pro Ile Val Gly Val Gly Met Glu Lys
                85                  90                  95      
Leu Ile Ile Ser Thr Asn Glu His Ala Ser Thr Asp Gln Gly Lys Thr
            100                 105                 110         
Val Ser Arg Ala Thr Thr Asn Ser Lys Thr Glu Ser Asn Thr Ala Gly
        115                 120                 125             
Val Ser Val Asn Val Gly Tyr Gln Asn Gly Phe Thr Ala Asn Val Thr
    130                 135                 140                 
Thr Asn Tyr Ser His Thr Thr Asp Asn Ser Thr Ala Val Gln Asp Ser
145                 150                 155                 160 
Asn Gly Glu Ser Trp Asn Thr Gly Leu Ser Ile Asn Lys Gly Glu Ser
                165                 170                 175     
Ala Tyr Ile Asn Ala Asn Val Arg Tyr Tyr Asn Thr Gly Thr Ala Pro
            180                 185                 190         
Met Tyr Lys Val Thr Pro Thr Thr Asn Leu Val Leu Asp Gly Asp Thr
        195                 200                 205             
Leu Ser Thr Ile Lys Ala Gln Glu Asn Gln Ile Gly Asn Asn Leu Ser
    210                 215                 220                 
Pro Gly Asp Thr Tyr Pro Lys Lys Gly Leu Ser Pro Leu Ala Leu Asn
225                 230                 235                 240 
Thr Met Asp Gln Phe Ser Ser Arg Leu Ile Pro Ile Asn Tyr Asp Gln
                245                 250                 255     
Leu Lys Lys Leu Asp Ala Gly Lys Gln Ile Lys Leu Glu Thr Thr Gln
            260                 265                 270         
Val Ser Gly Asn Phe Gly Thr Lys Asn Ser Ser Gly Gln Ile Val Thr
        275                 280                 285             
Glu Gly Asn Ser Trp Ser Asp Tyr Ile Ser Gln Ile Asp Ser Ile Ser
    290                 295                 300                 
Ala Ser Ile Ile Leu Asp Thr Glu Asn Glu Ser Tyr Glu Arg Arg Val
305                 310                 315                 320 
Thr Ala Lys Asn Leu Gln Asp Pro Glu Asp Lys Thr Pro Glu Leu Thr
                325                 330                 335     
Ile Gly Glu Ala Ile Glu Lys Ala Phe Gly Ala Thr Lys Lys Asp Gly
            340                 345                 350         
Leu Leu Tyr Phe Asn Asp Ile Pro Ile Asp Glu Ser Cys Val Glu Leu
        355                 360                 365             
Ile Phe Asp Asp Asn Thr Ala Asn Lys Ile Lys Asp Ser Leu Lys Thr
    370                 375                 380                 
Leu Ser Asp Lys Lys Ile Tyr Asn Val Lys Leu Glu Arg Gly Met Asn
385                 390                 395                 400 
Ile Leu Ile Lys Thr Pro Thr Tyr Phe Thr Asn Phe Asp Asp Tyr Asn
                405                 410                 415     
Asn Tyr Pro Ser Thr Trp Ser Asn Val Asn Thr Thr Asn Gln Asp Gly
            420                 425                 430         
Leu Gln Gly Ser Ala Asn Lys Leu Asn Gly Glu Thr Lys Ile Lys Ile
        435                 440                 445             
Pro Met Ser Glu Leu Lys Pro Tyr Lys Arg Tyr Val Phe Ser Gly Tyr
    450                 455                 460                 
Ser Lys Asp Pro Leu Thr Ser Asn Ser Ile Ile Val Lys Ile Lys Ala
465                 470                 475                 480 
Lys Glu Glu Lys Thr Asp Tyr Leu Val Pro Glu Gln Gly Tyr Thr Lys
                485                 490                 495     
Phe Ser Tyr Glu Phe Glu Thr Thr Glu Lys Asp Ser Ser Asn Ile Glu
            500                 505                 510         
Ile Thr Leu Ile Gly Ser Gly Thr Thr Tyr Leu Asp Asn Leu Ser Ile
        515                 520                 525             
Thr Glu Leu Asn Ser Thr Pro Glu Ile Leu Asp Glu Pro Glu Val Lys
    530                 535                 540                 
Ile Pro Thr Asp Gln Glu Ile Met Asp Ala His Lys Ile Tyr Phe Ala
545                 550                 555                 560 
Asp Leu Asn Phe Asn Pro Ser Thr Gly Asn Thr Tyr Ile Asn Gly Met
                565                 570                 575     
Tyr Phe Ala Pro Thr Gln Thr Asn Lys Glu Ala Leu Asp Tyr Ile Gln
            580                 585                 590         
Lys Tyr Arg Val Glu Ala Thr Leu Gln Tyr Ser Gly Phe Lys Asp Ile
        595                 600                 605             
Gly Thr Lys Asp Lys Glu Met Arg Asn Tyr Leu Gly Asp Pro Asn Gln
    610                 615                 620                 
Pro Lys Thr Asn Tyr Val Asn Leu Arg Ser Tyr Phe Thr Gly Gly Glu
625                 630                 635                 640 
Asn Ile Met Thr Tyr Lys Lys Leu Arg Ile Tyr Ala Ile Thr Pro Asp
                645                 650                 655     
Asp Arg Glu Leu Leu Val Leu Ser Val Asp His His His His His His
            660                 665                 670         


<210> 52
<211> 427
<212> PRT
<213> Artificial Sequence

<220> 
<223> amino acid sequence of CDTa without signal
      peptide, with five mutations (R345A, Q350A, N385A,
      R402A, S388F, aa 44-463) (C107)

<400> 52
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Ser Leu Asp Phe Lys Asp Asp
    210                 215                 220                 
Val Ser Lys Gly Asp Ser Trp Gly Lys Ala Asn Tyr Asn Asp Trp Ser
225                 230                 235                 240 
Asn Lys Leu Thr Pro Asn Glu Leu Ala Asp Val Asn Asp Tyr Met Arg
                245                 250                 255     
Gly Gly Tyr Thr Ala Ile Asn Asn Tyr Leu Ile Ser Asn Gly Pro Val
            260                 265                 270         
Asn Asn Pro Asn Pro Glu Leu Asp Ser Lys Ile Thr Asn Ile Glu Asn
        275                 280                 285             
Ala Leu Lys Arg Glu Pro Ile Pro Thr Asn Leu Thr Val Tyr Ala Arg
    290                 295                 300                 
Ser Gly Pro Ala Glu Phe Gly Leu Thr Leu Thr Ser Pro Glu Tyr Asp
305                 310                 315                 320 
Phe Asn Lys Leu Glu Asn Ile Asp Ala Phe Lys Ser Lys Trp Glu Gly
                325                 330                 335     
Gln Ala Leu Ser Tyr Pro Ala Phe Ile Phe Thr Ser Ile Gly Ser Val
            340                 345                 350         
Asn Met Ser Ala Phe Ala Lys Ala Lys Ile Val Leu Arg Ile Thr Ile
        355                 360                 365             
Pro Lys Gly Ser Pro Gly Ala Tyr Leu Ser Ala Ile Pro Gly Tyr Ala
    370                 375                 380                 
Gly Glu Tyr Glu Val Leu Leu Asn His Gly Ser Lys Phe Lys Ile Asn
385                 390                 395                 400 
Lys Ile Asp Ser Tyr Lys Asp Gly Thr Ile Thr Lys Leu Ile Val Asp
                405                 410                 415     
Ala Thr Leu Ile Pro His His His His His His
            420                 425         


<210> 53
<211> 1287
<212> DNA
<213> Artificial Sequence

<220> 
<223> Polynucleotide sequence of CDTa without signal
      peptide, with five mutations (R345A, Q350A, N385A,
      R402A, S388F, aa 44-463) (C107)

<400> 53
atggtttgca ataccaccta taaagcaccg attgaacgtc cggaagattt tctgaaagat 60
aaagaaaaag ccaaagaatg ggaacgcaaa gaagcagaac gtattgaaca gaaactggaa 120
cgtagcgaaa aagaagcact ggaaagctac aaaaaagata gcgtggaaat ttcaaaatat 180
agccagaccc gcaattattt ctatgattat cagattgaag ccaatagccg tgaaaaagaa 240
tataaagaac tgcgcaatgc cattagcaaa aacaaaattg ataaaccgat gtatgtgtat 300
tatttcgaaa gtccggaaaa atttgccttt aacaaagtga ttcgcaccga aaatcagaat 360
gaaattagcc tggaaaaatt caatgaattt aaagaaacca ttcagaataa actgtttaaa 420
caggatggct ttaaagatat ttcactgtat gaaccgggta aaggtgatga aaaaccgaca 480
ccgctgctga tgcatctgaa actgcctcgt aataccggta tgctgccgta taccaatacc 540
aataatgtta gcaccctgat tgaacagggc tatagcatca aaattgataa aattgtgcgc 600
attgtgattg atggcaaaca ttatatcaaa gccgaagcca gcgttgtttc aagcctggat 660
tttaaagatg atgtgagcaa aggcgatagc tggggtaaag caaactataa tgattggagc 720
aataaactga ccccgaatga actggcagat gtgaatgatt atatgcgtgg tggttatacc 780
gccattaaca attatctgat tagcaatggt ccggtgaata atccgaatcc ggaactggat 840
agcaaaatta ccaatattga aaatgccctg aaacgcgaac cgattccgac caatctgacc 900
gtttatgcac gtagcggtcc ggcagaattt ggtctgaccc tgaccagtcc ggaatatgac 960
tttaacaaac tggaaaatat tgatgccttt aaaagcaaat gggaaggtca ggcactgagc 1020
tatccggcat ttattttcac cagcattggt agcgttaata tgagcgcatt tgccaaagca 1080
aaaattgtgc tgcgtattac cattccgaaa ggtagtccgg gtgcatatct gagcgcaatt 1140
ccgggttatg ccggtgaata tgaagttctg ctgaatcatg gcagcaaatt caaaattaac 1200
aaaattgata gctataaaga tggcaccatt accaaactga ttgttgatgc aaccctgatt 1260
ccgcaccacc atcatcatca ttaataa                                     1287

<210> 54
<211> 427
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of CDTa without signal
      peptide, with six mutations (R345A, Q350A, N385A,
      R402A, S388F, E430Q, aa 44-463) (C108)

<400> 54
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Ser Leu Asp Phe Lys Asp Asp
    210                 215                 220                 
Val Ser Lys Gly Asp Ser Trp Gly Lys Ala Asn Tyr Asn Asp Trp Ser
225                 230                 235                 240 
Asn Lys Leu Thr Pro Asn Glu Leu Ala Asp Val Asn Asp Tyr Met Arg
                245                 250                 255     
Gly Gly Tyr Thr Ala Ile Asn Asn Tyr Leu Ile Ser Asn Gly Pro Val
            260                 265                 270         
Asn Asn Pro Asn Pro Glu Leu Asp Ser Lys Ile Thr Asn Ile Glu Asn
        275                 280                 285             
Ala Leu Lys Arg Glu Pro Ile Pro Thr Asn Leu Thr Val Tyr Ala Arg
    290                 295                 300                 
Ser Gly Pro Ala Glu Phe Gly Leu Thr Leu Thr Ser Pro Glu Tyr Asp
305                 310                 315                 320 
Phe Asn Lys Leu Glu Asn Ile Asp Ala Phe Lys Ser Lys Trp Glu Gly
                325                 330                 335     
Gln Ala Leu Ser Tyr Pro Ala Phe Ile Phe Thr Ser Ile Gly Ser Val
            340                 345                 350         
Asn Met Ser Ala Phe Ala Lys Ala Lys Ile Val Leu Arg Ile Thr Ile
        355                 360                 365             
Pro Lys Gly Ser Pro Gly Ala Tyr Leu Ser Ala Ile Pro Gly Tyr Ala
    370                 375                 380                 
Gly Glu Tyr Gln Val Leu Leu Asn His Gly Ser Lys Phe Lys Ile Asn
385                 390                 395                 400 
Lys Ile Asp Ser Tyr Lys Asp Gly Thr Ile Thr Lys Leu Ile Val Asp
                405                 410                 415     
Ala Thr Leu Ile Pro His His His His His His
            420                 425         


<210> 55
<211> 1287
<212> DNA
<213> Artificial Sequence

<220> 
<223> Polynucleotide sequence of CDTa without signal
      peptide, with six mutations (R345A, Q350A, N385A,
      R402A, S388F, E430Q, aa 44-463) (C108)

<400> 55
atggtttgca ataccaccta taaagcaccg attgaacgtc cggaagattt tctgaaagat 60
aaagaaaaag ccaaagaatg ggaacgcaaa gaagcagaac gtattgaaca gaaactggaa 120
cgtagcgaaa aagaagcact ggaaagctac aaaaaagata gcgtggaaat ttcaaaatat 180
agccagaccc gcaattattt ctatgattat cagattgaag ccaatagccg tgaaaaagaa 240
tataaagaac tgcgcaatgc cattagcaaa aacaaaattg ataaaccgat gtatgtgtat 300
tatttcgaaa gtccggaaaa atttgccttt aacaaagtga ttcgcaccga aaatcagaat 360
gaaattagcc tggaaaaatt caatgaattt aaagaaacca ttcagaataa actgtttaaa 420
caggatggct ttaaagatat ttcactgtat gaaccgggta aaggtgatga aaaaccgaca 480
ccgctgctga tgcatctgaa actgcctcgt aataccggta tgctgccgta taccaatacc 540
aataatgtta gcaccctgat tgaacagggc tatagcatca aaattgataa aattgtgcgc 600
attgtgattg atggcaaaca ttatatcaaa gccgaagcca gcgttgtttc aagcctggat 660
tttaaagatg atgtgagcaa aggcgatagc tggggtaaag caaactataa tgattggagc 720
aataaactga ccccgaatga actggcagat gtgaatgatt atatgcgtgg tggttatacc 780
gccattaaca attatctgat tagcaatggt ccggtgaata atccgaatcc ggaactggat 840
agcaaaatta ccaatattga aaatgccctg aaacgcgaac cgattccgac caatctgacc 900
gtttatgcac gtagcggtcc ggcagaattt ggtctgaccc tgaccagtcc ggaatatgac 960
tttaacaaac tggaaaatat tgatgccttt aaaagcaaat gggaaggtca ggcactgagc 1020
tatccggcat ttattttcac cagcattggt agcgttaata tgagcgcatt tgccaaagca 1080
aaaattgtgc tgcgtattac cattccgaaa ggtagtccgg gtgcatatct gagcgcaatt 1140
ccgggttatg ccggtgaata tcaagttctg ctgaatcatg gcagcaaatt caaaattaac 1200
aaaattgata gctataaaga tggcaccatt accaaactga ttgttgatgc aaccctgatt 1260
ccgcaccacc atcatcatca ttaataa                                     1287

<210> 56
<211> 427
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of CDTa without signal
      peptide, with six mutations (R345A, Q350A, N385A,
      R402A, S388F, E428Q, aa 44-463) (C110

<400> 56
Met Val Cys Asn Thr Thr Tyr Lys Ala Pro Ile Glu Arg Pro Glu Asp
 1               5                  10                  15      
Phe Leu Lys Asp Lys Glu Lys Ala Lys Glu Trp Glu Arg Lys Glu Ala
            20                  25                  30          
Glu Arg Ile Glu Gln Lys Leu Glu Arg Ser Glu Lys Glu Ala Leu Glu
        35                  40                  45              
Ser Tyr Lys Lys Asp Ser Val Glu Ile Ser Lys Tyr Ser Gln Thr Arg
    50                  55                  60                  
Asn Tyr Phe Tyr Asp Tyr Gln Ile Glu Ala Asn Ser Arg Glu Lys Glu
65                  70                  75                  80  
Tyr Lys Glu Leu Arg Asn Ala Ile Ser Lys Asn Lys Ile Asp Lys Pro
                85                  90                  95      
Met Tyr Val Tyr Tyr Phe Glu Ser Pro Glu Lys Phe Ala Phe Asn Lys
            100                 105                 110         
Val Ile Arg Thr Glu Asn Gln Asn Glu Ile Ser Leu Glu Lys Phe Asn
        115                 120                 125             
Glu Phe Lys Glu Thr Ile Gln Asn Lys Leu Phe Lys Gln Asp Gly Phe
    130                 135                 140                 
Lys Asp Ile Ser Leu Tyr Glu Pro Gly Lys Gly Asp Glu Lys Pro Thr
145                 150                 155                 160 
Pro Leu Leu Met His Leu Lys Leu Pro Arg Asn Thr Gly Met Leu Pro
                165                 170                 175     
Tyr Thr Asn Thr Asn Asn Val Ser Thr Leu Ile Glu Gln Gly Tyr Ser
            180                 185                 190         
Ile Lys Ile Asp Lys Ile Val Arg Ile Val Ile Asp Gly Lys His Tyr
        195                 200                 205             
Ile Lys Ala Glu Ala Ser Val Val Ser Ser Leu Asp Phe Lys Asp Asp
    210                 215                 220                 
Val Ser Lys Gly Asp Ser Trp Gly Lys Ala Asn Tyr Asn Asp Trp Ser
225                 230                 235                 240 
Asn Lys Leu Thr Pro Asn Glu Leu Ala Asp Val Asn Asp Tyr Met Arg
                245                 250                 255     
Gly Gly Tyr Thr Ala Ile Asn Asn Tyr Leu Ile Ser Asn Gly Pro Val
            260                 265                 270         
Asn Asn Pro Asn Pro Glu Leu Asp Ser Lys Ile Thr Asn Ile Glu Asn
        275                 280                 285             
Ala Leu Lys Arg Glu Pro Ile Pro Thr Asn Leu Thr Val Tyr Ala Arg
    290                 295                 300                 
Ser Gly Pro Ala Glu Phe Gly Leu Thr Leu Thr Ser Pro Glu Tyr Asp
305                 310                 315                 320 
Phe Asn Lys Leu Glu Asn Ile Asp Ala Phe Lys Ser Lys Trp Glu Gly
                325                 330                 335     
Gln Ala Leu Ser Tyr Pro Ala Phe Ile Phe Thr Ser Ile Gly Ser Val
            340                 345                 350         
Asn Met Ser Ala Phe Ala Lys Ala Lys Ile Val Leu Arg Ile Thr Ile
        355                 360                 365             
Pro Lys Gly Ser Pro Gly Ala Tyr Leu Ser Ala Ile Pro Gly Tyr Ala
    370                 375                 380                 
Gly Gln Tyr Glu Val Leu Leu Asn His Gly Ser Lys Phe Lys Ile Asn
385                 390                 395                 400 
Lys Ile Asp Ser Tyr Lys Asp Gly Thr Ile Thr Lys Leu Ile Val Asp
                405                 410                 415     
Ala Thr Leu Ile Pro His His His His His His
            420                 425         

