                         SEQUENCE LISTING

<110>  Sandoz AG
       Boehringer Ingelheim RCV GmbH & Co KG
 
<120>  Method for producing a recombinant protein of interest

<130>  R 65201

<150>  ep12198007.2
<151>  2012-12-19

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CSFV-derived

<400>  1

Met Glu Pro Val Tyr Asp Thr Ala Gly Arg Pro Leu Phe Gly Asn Pro 
1               5                   10                  15      


Ser Glu Val His Pro Gln Ser Thr Leu Lys Leu Pro His Asp Arg Gly 
            20                  25                  30          


Arg Gly Asp Ile Arg Thr Thr Leu Arg Asp Leu Pro Arg Lys Gly Asp 
        35                  40                  45              


Cys Arg Ser Gly Asn His Leu Gly Pro Val Ser Gly Ile Tyr Ile Lys 
    50                  55                  60                  


Pro Gly Pro Val Tyr Tyr Gln Asp Tyr Thr Gly Pro Val Tyr His Arg 
65                  70                  75                  80  


Ala Pro Leu Glu Phe Phe His His His His His His 
                85                  90          


<210>  2
<211>  72
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CSFV-derived

<400>  2

Met His His His His His His Glu Thr Thr Lys Arg Ile Gly Arg Val 
1               5                   10                  15      


Thr Gly Ser Asp Gly Lys Leu Tyr His Ile Tyr Val Glu Val Asp Gly 
            20                  25                  30          


Glu Ile Leu Leu Lys Leu Ala Lys Arg Gly Thr Pro Arg Thr Leu Lys 
        35                  40                  45              


Trp Thr Arg Asn Thr Thr Asn Cys Pro Leu Trp Val Thr Ser Cys Ser 
    50                  55                  60                  


Trp Ser His Pro Gln Phe Glu Lys 
65                  70          


<210>  3
<211>  66
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CSFV-derived

<400>  3

Met Glu Thr Thr Lys Arg Ile Gly Arg Val Thr Gly Ser Asp Gly Lys 
1               5                   10                  15      


Leu Tyr His Ile Tyr Val Glu Val Asp Gly Glu Ile Leu Leu Lys Leu 
            20                  25                  30          


Ala Lys Arg Gly Thr Pro Arg Thr Leu Lys Trp Thr Arg Asn Thr Thr 
        35                  40                  45              


Asn Cys Pro Leu Trp Val Thr Ser Cys Ser Trp Ser His Pro Gln Phe 
    50                  55                  60                  


Glu Lys 
65      


<210>  4
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CSFV-derived

<400>  4

Met Glu Thr Thr Lys Arg Ile Gly Arg Val Thr Gly Ser Asp Gly Lys 
1               5                   10                  15      


Leu Tyr His Ile Tyr Val Glu Val Asp Gly Glu Ile Leu Leu Lys Leu 
            20                  25                  30          


Ala Lys Arg Gly Thr Pro Arg Thr Leu Lys Trp Thr Arg Asn Thr Thr 
        35                  40                  45              


Asn Cys Pro Leu Trp Val Thr Ser Cys Ser Val Asp Lys Leu Ala Ala 
    50                  55                  60                  


Ala Leu Glu His His His His His His 
65                  70              


<210>  5
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CSFV-derived

<400>  5

Met Glu Thr Thr Lys Arg Ile Gly Arg Val Thr Gly Ser Asp Gly Lys 
1               5                   10                  15      


Leu Tyr His Ile Tyr Val Glu Val Asp Gly Glu Ile Leu Leu Lys Leu 
            20                  25                  30          


Ala Lys Arg Gly Thr Pro Arg Thr Leu Lys Trp Thr Arg Asn Thr Thr 
        35                  40                  45              


Asn Cys Pro Leu Trp Val Thr Ser Cys Met Ala Arg Val Gln Phe Lys 
    50                  55                  60                  


Gln Arg Glu Ser Thr Asp Ala Ile Phe Val His Cys Ser Ala Thr Lys 
65                  70                  75                  80  


Pro Ser Gln Asn Val Gly Val Arg Glu Ile Arg Gln Trp His Lys Glu 
                85                  90                  95      


Gln Gly Trp Leu Asp Val Gly Tyr His Phe Ile Ile Lys Arg Asp Gly 
            100                 105                 110         


Thr Val Glu Ala Gly Arg Asp Glu Met Ala Val Gly Ser His Ala Lys 
        115                 120                 125             


Gly Tyr Asn His Asn Ser Ile Gly Val Cys Leu Val Gly Gly Ile Asp 
    130                 135                 140                 


Asp Lys Gly Lys Phe Asp Ala Asn Phe Thr Pro Ala Gln Met Gln Ser 
145                 150                 155                 160 


Leu Arg Ser Leu Leu Val Thr Leu Leu Ala Lys Tyr Glu Gly Ala Gly 
                165                 170                 175     


Leu Arg Ala His His Glu Val Ala Pro Lys Ala Cys Pro Ser Phe Asp 
            180                 185                 190         


Leu Lys Arg Trp Trp Glu Lys Asn Glu Leu Val Thr Ser Asp Arg Gly 
        195                 200                 205             


His His His His His His 
    210                 


<210>  6
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CSFV-derived

<400>  6
gaaattaata cgactcacta tagg                                              24


<210>  7
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CSFV-derived

<400>  7
atatagtcga cttattaaaa gaattccaga gg                                     32


<210>  8
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CSFV-derived

<400>  8
atagtcgact tattagtgat ggtgatggtg atgaaagaat tccagagg                    48


<210>  9
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CSFV-derived

<400>  9
tatacatatg gaaaccacta aacgtattgg c                                      31


<210>  10
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CSFV-derived

<400>  10
tatacatatg catcaccatc accatcacga aaccactaaa cgtattggc                   49


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CSFV-derived

<400>  11
gttatgctag ttattgctca gcgg                                              24


<210>  12
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  model peptide pep6His

<400>  12

Ser Val Asp Lys Leu Ala Ala Ala Leu Glu His His His His His His 
1               5                   10                  15      


<210>  13
<211>  148
<212>  PRT
<213>  artificial sequence

<220>
<223>  HoBi-Variant

<400>  13

Met Glu Pro Leu Tyr Asp Lys Asn Gly Ala Val Leu Phe Gly Glu Pro 
1               5                   10                  15      


Ser Asp Thr His Pro Gln Ser Thr Leu Lys Leu Pro His Pro Arg Gly 
            20                  25                  30          


Glu Lys Glu Val Ile Val Gly Ile Arg Asp Leu Pro Arg Lys Gly Asp 
        35                  40                  45              


Cys Arg Thr Gly Asn Arg Leu Gly Pro Val Ser Gly Leu Phe Val Lys 
    50                  55                  60                  


Pro Gly Pro Val Phe Tyr Gln Asp Tyr Ser Gly Pro Val Tyr His Arg 
65                  70                  75                  80  


Ala Pro Leu Glu Gln Phe Lys Gln Ala Pro Met Cys Glu Val Thr Lys 
                85                  90                  95      


Arg Ile Gly Arg Val Thr Gly Ser Asp Gly Asn Leu Tyr His Met Tyr 
            100                 105                 110         


Val Cys Thr Asp Gly Cys Ile Leu Val Lys Thr Ala Lys Arg Glu Gly 
        115                 120                 125             


Gln Asp Val Leu Lys Trp Val Tyr Asn Val Leu Asp Ser Pro Ile Trp 
    130                 135                 140                 


Val Thr Ser Cys 
145             


<210>  14
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  Npro Forward NdeI

<400>  14
cgcgacatat ggaactcaat catttcgaac                                        30


<210>  15
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  Npro 150 reverse SpeI

<400>  15
gctgcaacta gtgacc                                                       16


<210>  16
<211>  133
<212>  PRT
<213>  CSFV

<400>  16

Met Glu Thr Thr Lys Arg Ile Gly Arg Val Thr Gly Ser Asp Gly Lys 
1               5                   10                  15      


Leu Tyr His Ile Tyr Val Glu Val Asp Gly Glu Ile Leu Leu Lys Leu 
            20                  25                  30          


Ala Lys Arg Gly Thr Pro Arg Thr Leu Lys Trp Thr Arg Asn Thr Thr 
        35                  40                  45              


Asn Cys Pro Leu Trp Val Thr Ser Cys Gln Pro Asp Ala Ile Asn Ala 
    50                  55                  60                  


Pro Val Thr Cys Cys Tyr Asn Phe Thr Asn Arg Lys Ile Ser Val Gln 
65                  70                  75                  80  


Arg Leu Ala Ser Tyr Arg Arg Ile Thr Ser Ser Lys Cys Pro Lys Glu 
                85                  90                  95      


Ala Val Ile Phe Lys Thr Ile Val Ala Lys Glu Ile Cys Ala Asp Pro 
            100                 105                 110         


Lys Gln Lys Trp Val Gln Asp Ser Met Asp His Leu Asp Lys Gln Thr 
        115                 120                 125             


Gln Thr Pro Lys Thr 
    130             


<210>  17
<211>  224
<212>  PRT
<213>  CSFV

<400>  17

Met Glu Pro Val Tyr Asp Thr Ala Gly Arg Pro Leu Phe Gly Asn Pro 
1               5                   10                  15      


Ser Glu Val His Pro Gln Ser Thr Leu Lys Leu Pro His Asp Arg Gly 
            20                  25                  30          


Arg Gly Asp Ile Arg Thr Thr Leu Arg Asp Leu Pro Arg Lys Gly Asp 
        35                  40                  45              


Cys Arg Ser Gly Asn His Leu Gly Pro Val Ser Gly Ile Tyr Ile Lys 
    50                  55                  60                  


Pro Gly Pro Val Tyr Tyr Gln Asp Tyr Thr Gly Pro Val Tyr His Arg 
65                  70                  75                  80  


Ala Pro Leu Glu Phe Phe Asp Glu Thr Gln Phe Glu Glu Thr Thr Lys 
                85                  90                  95      


Arg Ile Gly Arg Val Thr Gly Ser Asp Gly Lys Leu Tyr His Ile Tyr 
            100                 105                 110         


Val Glu Val Asp Gly Glu Ile Leu Leu Lys Leu Ala Lys Arg Gly Thr 
        115                 120                 125             


Pro Arg Thr Leu Lys Trp Thr Arg Asn Thr Thr Asn Cys Pro Leu Trp 
    130                 135                 140                 


Val Thr Ser Cys Gln Pro Asp Ala Ile Asn Ala Pro Val Thr Cys Cys 
145                 150                 155                 160 


Tyr Asn Phe Thr Asn Arg Lys Ile Ser Val Gln Arg Leu Ala Ser Tyr 
                165                 170                 175     


Arg Arg Ile Thr Ser Ser Lys Cys Pro Lys Glu Ala Val Ile Phe Lys 
            180                 185                 190         


Thr Ile Val Ala Lys Glu Ile Cys Ala Asp Pro Lys Gln Lys Trp Val 
        195                 200                 205             


Gln Asp Ser Met Asp His Leu Asp Lys Gln Thr Gln Thr Pro Lys Thr 
    210                 215                 220                 


<210>  18
<211>  76
<212>  PRT
<213>  CSFV

<400>  18

Gln Pro Asp Ala Ile Asn Ala Pro Val Thr Cys Cys Tyr Asn Phe Thr 
1               5                   10                  15      


Asn Arg Lys Ile Ser Val Gln Arg Leu Ala Ser Tyr Arg Arg Ile Thr 
            20                  25                  30          


Ser Ser Lys Cys Pro Lys Glu Ala Val Ile Phe Lys Thr Ile Val Ala 
        35                  40                  45              


Lys Glu Ile Cys Ala Asp Pro Lys Gln Lys Trp Val Gln Asp Ser Met 
    50                  55                  60                  


Asp His Leu Asp Lys Gln Thr Gln Thr Pro Lys Thr 
65                  70                  75      


<210>  19
<211>  164
<212>  PRT
<213>  CSFV

<400>  19

Met Glu Pro Val Tyr Asp Thr Ala Gly Arg Pro Leu Phe Gly Asn Pro 
1               5                   10                  15      


Ser Glu Val His Pro Gln Ser Thr Leu Lys Leu Pro His Asp Arg Gly 
            20                  25                  30          


Arg Gly Asp Ile Arg Thr Thr Leu Arg Asp Leu Pro Arg Lys Gly Asp 
        35                  40                  45              


Cys Arg Ser Gly Asn His Leu Gly Pro Val Ser Gly Ile Tyr Ile Lys 
    50                  55                  60                  


Pro Gly Pro Val Tyr Tyr Gln Asp Tyr Thr Gly Pro Val Tyr His Arg 
65                  70                  75                  80  


Ala Pro Leu Glu Phe Phe Asp Glu Thr Gln Phe Glu Glu Thr Thr Lys 
                85                  90                  95      


Arg Ile Gly Arg Val Thr Gly Ser Asp Gly Lys Leu Tyr His Ile Tyr 
            100                 105                 110         


Val Glu Val Asp Gly Glu Ile Leu Leu Lys Leu Ala Lys Arg Gly Thr 
        115                 120                 125             


Pro Arg Thr Leu Lys Trp Thr Arg Asn Thr Thr Asn Cys Pro Leu Trp 
    130                 135                 140                 


Val Thr Ser Cys Ser Val Asp Lys Leu Ala Ala Ala Leu Glu His His 
145                 150                 155                 160 


His His His His 
                


<210>  20
<211>  16
<212>  PRT
<213>  CSFV

<400>  20

Ser Val Asp Lys Leu Ala Ala Ala Leu Glu His His His His His His 
1               5                   10                  15      


<210>  21
<211>  168
<212>  PRT
<213>  CSFV

<400>  21

Met Glu Leu Leu Asn Phe Glu Leu Leu Tyr Lys Thr Tyr Lys Gln Lys 
1               5                   10                  15      


Pro Ala Gly Val Gln Glu Pro Leu Tyr Asp Lys Asn Gly Ala Val Leu 
            20                  25                  30          


Phe Gly Glu Pro Ser Asp Thr His Pro Gln Ser Thr Leu Lys Leu Pro 
        35                  40                  45              


His Pro Arg Gly Glu Lys Glu Val Ile Val Gly Ile Arg Asp Leu Pro 
    50                  55                  60                  


Arg Lys Gly Asp Cys Arg Thr Gly Asn Arg Leu Gly Pro Val Ser Gly 
65                  70                  75                  80  


Leu Phe Val Lys Pro Gly Pro Val Phe Tyr Gln Asp Tyr Ser Gly Pro 
                85                  90                  95      


Val Tyr His Arg Ala Pro Leu Glu Gln Phe Lys Gln Ala Pro Met Cys 
            100                 105                 110         


Glu Val Thr Lys Arg Ile Gly Arg Val Thr Gly Ser Asp Gly Asn Leu 
        115                 120                 125             


Tyr His Met Tyr Val Cys Thr Asp Gly Cys Ile Leu Val Lys Thr Ala 
    130                 135                 140                 


Lys Arg Glu Gly Gln Asp Val Leu Lys Trp Val Tyr Asn Val Leu Asp 
145                 150                 155                 160 


Ser Pro Ile Trp Val Ala Ser Cys 
                165             


