                         SEQUENCE LISTING

<110>  Boehringer Ingelheim Vetmedica GmbH
 
<120>  Novel recombinant outer membrane proteins from Brachyspira 
       hyodysenteriae and uses thereof

<130>  P01-2891

<160>  18    

<170>  PatentIn version 3.3

<210>  1
<211>  210
<212>  PRT
<213>  Brachyspira hyodysenteriae

<400>  1

Met Leu Ile Ile Thr Cys Phe Met Ser Ser Ser Leu Phe Pro Leu Ile 
1               5                   10                  15      


Asp Ala Phe Tyr Leu Ala Pro Lys Val Ile Tyr Asn Phe Asn Asp Ser 
            20                  25                  30          


Gly Phe Arg Asn Ser Lys Asn Gln Arg Val Met Asn Asn Tyr Leu Gly 
        35                  40                  45              


Ala Gly Phe Ser Ala Gly Phe Asp Phe Tyr Arg Tyr Gln Arg Asn Ile 
    50                  55                  60                  


Pro Leu Arg Val Glu Leu Glu Tyr Thr Phe Lys Asp Gly Met Thr Gly 
65                  70                  75                  80  


Asn Tyr His Pro Ala Asn Ile Val Lys Gln Ser Gln His Ser Ile Leu 
                85                  90                  95      


Ala Ala Ala Tyr Tyr Ser Met His Ile Tyr His Ile Lys Lys Ser Glu 
            100                 105                 110         


Leu Arg Thr Ile Thr Ala Glu Glu Ile Tyr Ser Arg Thr Pro Ile Met 
        115                 120                 125             


Ser Leu Tyr Leu Gly Leu Leu Met Gly Thr Lys Ile Asn Ala Asn Thr 
    130                 135                 140                 


Tyr Asp Arg Trp Phe Glu Glu Asn Gly Arg Val Lys Ala Thr Val Thr 
145                 150                 155                 160 


Val Pro Ser Pro Thr Phe Ala Ile Gly Gly Ala Val Gly Val Asp Ile 
                165                 170                 175     


Tyr Val Thr Ser Phe Leu Asn Leu Asp Ile Gly Tyr Arg Ile Leu Tyr 
            180                 185                 190         


Gly Leu Asp Ser Val Leu Ser His Glu Phe Ala Ile Ala Ala Arg Phe 
        195                 200                 205             


Pro Ile 
    210 


<210>  2
<211>  630
<212>  DNA
<213>  Brachyspira hyodysenteriae

<400>  2
atgttaataa taacttgctt tatgagttca tctttgtttc ctctaataga tgcattttat     60

ttagcaccta aagttatata taattttaat gactcaggtt tcagaaatag taaaaatcag    120

agagttatga ataattatct aggagctgga ttttctgcag gatttgactt ctatagatac    180

caaagaaata tacctttgag agtagaatta gagtatactt ttaaagatgg tatgactgga    240

aattatcacc ctgcaaatat agtaaaacaa tctcagcatt ctatattagc agctgcttat    300

tacagcatgc atatatatca tatcaaaaaa agcgaattaa gaactattac agctgaagaa    360

atatatagca gaacgccaat aatgtctcta tatttagggc tgttaatggg tacaaaaata    420

aatgcaaata cttatgatag atggtttgaa gaaaatggaa gagttaaagc tactgtaaca    480

gtgcctagcc ctacatttgc aataggcggt gctgttggag ttgatatata tgttactagc    540

ttccttaact tggatatagg atacagaata ttatatggtt tggatagtgt tttatcacat    600

gaatttgcta tagcagcaag attcccaata                                     630


<210>  3
<211>  123
<212>  PRT
<213>  Brachyspira hyodysenteriae

<400>  3

Met Phe Ser Leu Leu Ile Phe Val Ile Ala Ala Cys Lys Ser Thr Pro 
1               5                   10                  15      


Thr Ser Thr Thr Pro Glu Asp Val Val Ile Ala Asp Asp Pro Ala Pro 
            20                  25                  30          


Ala Val Ala Glu Glu Glu Pro Lys Pro Glu Glu Val Val Ala Asp Thr 
        35                  40                  45              


Lys Thr Leu Ala Tyr Gly Ser Glu Glu Leu Tyr Leu Pro Met Asp Thr 
    50                  55                  60                  


Ser Ile Arg Asp Thr Glu Arg Gly Arg Ile Leu Glu Thr Thr Pro Lys 
65                  70                  75                  80  


Val Ile Phe Lys Phe Val Glu Thr Asn Met Pro Ala Thr Ala Glu Met 
                85                  90                  95      


Ser Phe Asn Gln Val Val Glu Phe Leu Glu Lys Asn Pro Asn Val Asn 
            100                 105                 110         


Ile Val Leu Glu Ala His Thr Ser Asn Arg Gly 
        115                 120             


<210>  4
<211>  369
<212>  DNA
<213>  Brachyspira hyodysenteriae

<400>  4
atgtttagtt tattgatctt tgtcatagca gcatgtaaaa gtacgcctac aagtacaaca     60

ccggaagatg tagttatagc agatgatcct gcccctgcag tagcagaaga agaaccaaaa    120

ccagaagaag tagtagctga tactaaaaca ttggcatatg gttctgaaga gctttatcta    180

cctatggata cttctataag agatactgaa agaggaagaa tactagaaac tactccaaaa    240

gtaatattca aatttgtaga gactaatatg cctgcaacag cagaaatgtc attcaatcaa    300

gttgtagagt ttttagagaa aaatcctaat gtgaatatag ttttggaagc tcatacaagc    360

aacagaggt                                                            369


<210>  5
<211>  189
<212>  PRT
<213>  Brachyspira hyodysenteriae

<400>  5

Met Leu Leu Met Ser Val Val Ile Ile Ala Ala Ala Cys Lys Ser Ala 
1               5                   10                  15      


Pro Val Ala Thr Gly Pro Glu Asp Thr Ser Gly Phe Lys Thr Thr Glu 
            20                  25                  30          


Ala Asn Asn Gln Asn Ala Lys Gly Leu Asn Leu Pro Asp Gly Ala Thr 
        35                  40                  45              


Val Arg Glu Thr Pro Arg Gly Lys Val Leu Val Leu His Asp Pro Lys 
    50                  55                  60                  


Ser Lys Thr Asp Val Gly Lys Pro Gly Ser Thr Tyr Glu Val Lys Phe 
65                  70                  75                  80  


Gly Phe Asp Asn Thr Ile Glu Ile Gly Thr Tyr Lys Glu Ala Tyr Asn 
                85                  90                  95      


Leu Val Tyr Gln Ile Ile Asn Asn Asn Pro Gly Val Arg Ile Met Val 
            100                 105                 110         


Glu Gly Asn Ser Ser Lys Glu Gly Pro Ala Pro Tyr Asn Tyr Lys Leu 
        115                 120                 125             


Ser Gln Arg Arg Ser Asp Lys Ser Phe Asn Tyr Ile Ile Lys Leu Gly 
    130                 135                 140                 


Val Glu Asn Ser Lys Leu Leu Lys Asn Ala Phe Gly Glu Ala Leu Pro 
145                 150                 155                 160 


Glu Tyr Pro Thr Leu Lys Glu Asn Arg Arg Ser Glu Phe Ile Ile Ile 
                165                 170                 175     


Met Thr Glu Asp Asp Leu Lys Lys Tyr Asn Asp Phe Ala 
            180                 185                 


<210>  6
<211>  567
<212>  DNA
<213>  Brachyspira hyodysenteriae

<400>  6
atgttactta tgtcagtagt tattatagca gctgcatgta aaagtgctcc tgttgcaacc     60

ggtcctgaag acacaagcgg tttcaaaaca acagaagcta ataatcaaaa tgctaaagga    120

cttaacttac ctgacggagc tactgtaaga gaaactccaa gaggtaaagt attagtatta    180

catgatccta aatctaaaac agatgttggt aaacctggtt caacttatga ggtaaaattt    240

ggatttgata atactataga aataggaaca tataaagagg cttataattt agtttatcag    300

ataataaata ataatcctgg tgttagaata atggtggaag gtaattcaag taaagaaggc    360

cctgctcctt ataactataa actatctcag agaagatctg ataaaagctt caattatata    420

ataaaattag gtgtagaaaa cagcaaatta ttaaaaaatg cttttggaga ggctttgcct    480

gaatacccta ctcttaaaga aaatagaaga agcgaattta taataataat gactgaagat    540

gatttgaaaa agtataacga ttttgcc                                        567


<210>  7
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  7
gaaccaaaac cagaagaagt ag                                              22


<210>  8
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  8
tttagccctt acaacagaaa g                                               21


<210>  9
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  9
ttttacttat gtcagtagtt attatagcag                                      30


<210>  10
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  10
aaatcgttat actttttcaa atcatc                                          26


<210>  11
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  11
ttcagaaata gtaaaaatca gagag                                           25


<210>  12
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  12
ttgggaatct tgctgc                                                     16


<210>  13
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  13
ttactcgagt ttagtttatt gatctttgtc atag                                 34


<210>  14
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  14
actgaattca gtttccctga tatgtacttt c                                    31


<210>  15
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  15
aaactcgagt tacttatgtc agtagttatt atagcag                              37


<210>  16
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  16
tcggaattct tggcaaaatc gttatacttt                                      30


<210>  17
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  17
ttactcgagt taataataac ttgctttatg agttc                                35


<210>  18
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  18
ctagattcgg tattgggaat cttgctg                                         27


