                         SEQUENCE LISTING

<110>  Lonza Ltd
 
<120>  Method for preparation of 3-hydroxytyrosol

<130>  LP2319PC00

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  446
<212>  PRT
<213>  Nocardia iowensis

<400>  1

Tyr Arg Glu Leu Trp Gln Arg Val Gly Glu Val Ala Ala Ala Trp His 
1               5                   10                  15      


His Asp Pro Glu Asn Pro Leu Arg Ala Gly Asp Phe Val Ala Leu Leu 
            20                  25                  30          


Gly Phe Thr Ser Ile Asp Tyr Ala Thr Leu Asp Leu Ala Asp Ile His 
        35                  40                  45              


Leu Gly Ala Val Thr Val Pro Leu Gln Ala Ser Ala Ala Val Ser Gln 
    50                  55                  60                  


Leu Ile Ala Ile Leu Thr Glu Thr Ser Pro Arg Leu Leu Ala Ser Thr 
65                  70                  75                  80  


Pro Glu His Leu Asp Ala Ala Val Glu Cys Leu Leu Ala Gly Thr Thr 
                85                  90                  95      


Pro Glu Arg Leu Val Val Phe Asp Tyr His Pro Glu Asp Asp Asp Gln 
            100                 105                 110         


Arg Ala Ala Phe Glu Ser Ala Arg Arg Arg Leu Ala Asp Ala Gly Ser 
        115                 120                 125             


Leu Val Ile Val Glu Thr Leu Asp Ala Val Arg Ala Arg Gly Arg Asp 
    130                 135                 140                 


Leu Pro Ala Ala Pro Leu Phe Val Pro Asp Thr Asp Asp Asp Pro Leu 
145                 150                 155                 160 


Ala Leu Leu Ile Tyr Thr Ser Gly Ser Thr Gly Thr Pro Lys Gly Ala 
                165                 170                 175     


Met Tyr Thr Asn Arg Leu Ala Ala Thr Met Trp Gln Gly Asn Ser Met 
            180                 185                 190         


Leu Gln Gly Asn Ser Gln Arg Val Gly Ile Asn Leu Asn Tyr Met Pro 
        195                 200                 205             


Met Ser His Ile Ala Gly Arg Ile Ser Leu Phe Gly Val Leu Ala Arg 
    210                 215                 220                 


Gly Gly Thr Ala Tyr Phe Ala Ala Lys Ser Asp Met Ser Thr Leu Phe 
225                 230                 235                 240 


Glu Asp Ile Gly Leu Val Arg Pro Thr Glu Ile Phe Phe Val Pro Arg 
                245                 250                 255     


Val Cys Asp Met Val Phe Gln Arg Tyr Gln Ser Glu Leu Asp Arg Arg 
            260                 265                 270         


Ser Val Ala Gly Ala Asp Leu Asp Thr Leu Asp Arg Glu Val Lys Ala 
        275                 280                 285             


Asp Leu Arg Gln Asn Tyr Leu Gly Gly Arg Phe Leu Val Ala Val Val 
    290                 295                 300                 


Gly Ser Ala Pro Leu Ala Ala Glu Met Lys Thr Phe Met Glu Ser Val 
305                 310                 315                 320 


Leu Asp Leu Pro Leu His Asp Gly Tyr Gly Ser Thr Glu Ala Gly Ala 
                325                 330                 335     


Ser Val Leu Leu Asp Asn Gln Ile Gln Arg Pro Pro Val Leu Asp Tyr 
            340                 345                 350         


Lys Leu Val Asp Val Pro Glu Leu Gly Tyr Phe Arg Thr Asp Arg Pro 
        355                 360                 365             


His Pro Arg Gly Glu Leu Leu Leu Lys Ala Glu Thr Thr Ile Pro Gly 
    370                 375                 380                 


Tyr Tyr Lys Arg Pro Glu Val Thr Ala Glu Ile Phe Asp Glu Asp Gly 
385                 390                 395                 400 


Phe Tyr Lys Thr Gly Asp Ile Val Ala Glu Leu Glu His Asp Arg Leu 
                405                 410                 415     


Val Tyr Val Asp Arg Arg Asn Asn Val Leu Lys Leu Ser Gln Gly Glu 
            420                 425                 430         


Phe Val Thr Val Ala His Leu Glu Ala Val Phe Ala Ser Ser 
        435                 440                 445     


<210>  2
<211>  455
<212>  PRT
<213>  Nocardia iowensis

<400>  2

Tyr Arg Glu Leu Trp Gln Arg Val Gly Glu Val Ala Ala Ala Trp His 
1               5                   10                  15      


His Asp Pro Glu Asn Pro Leu Arg Ala Gly Asp Phe Val Ala Leu Leu 
            20                  25                  30          


Gly Phe Thr Ser Ile Asp Tyr Ala Thr Leu Asp Leu Ala Asp Ile His 
        35                  40                  45              


Leu Gly Ala Val Thr Val Pro Leu Gln Ala Ser Ala Ala Val Ser Gln 
    50                  55                  60                  


Leu Ile Ala Ile Leu Thr Glu Thr Ser Pro Arg Leu Leu Ala Ser Thr 
65                  70                  75                  80  


Pro Glu His Leu Asp Ala Ala Val Glu Cys Leu Leu Ala Gly Thr Thr 
                85                  90                  95      


Pro Glu Arg Leu Val Val Phe Asp Tyr His Pro Glu Asp Asp Asp Gln 
            100                 105                 110         


Arg Ala Ala Phe Glu Ser Ala Arg Arg Arg Leu Ala Asp Ala Gly Ser 
        115                 120                 125             


Leu Val Ile Val Glu Thr Leu Asp Ala Val Arg Ala Arg Gly Arg Asp 
    130                 135                 140                 


Leu Pro Ala Ala Pro Leu Phe Val Pro Asp Thr Asp Asp Asp Pro Leu 
145                 150                 155                 160 


Ala Leu Leu Ile Tyr Thr Ser Gly Ser Thr Gly Thr Pro Lys Gly Ala 
                165                 170                 175     


Met Tyr Thr Asn Arg Leu Ala Ala Thr Met Trp Gln Gly Asn Ser Met 
            180                 185                 190         


Leu Gln Gly Asn Ser Gln Arg Val Gly Ile Asn Leu Asn Tyr Met Pro 
        195                 200                 205             


Met Ser His Ile Ala Gly Arg Ile Ser Leu Phe Gly Val Leu Ala Arg 
    210                 215                 220                 


Gly Gly Thr Ala Tyr Phe Ala Ala Lys Ser Asp Met Ser Thr Leu Phe 
225                 230                 235                 240 


Glu Asp Ile Gly Leu Val Arg Pro Thr Glu Ile Phe Phe Val Pro Arg 
                245                 250                 255     


Val Cys Asp Met Val Phe Gln Arg Tyr Gln Ser Glu Leu Asp Arg Arg 
            260                 265                 270         


Ser Val Ala Gly Ala Asp Leu Asp Thr Leu Asp Arg Glu Val Lys Ala 
        275                 280                 285             


Asp Leu Arg Gln Asn Tyr Leu Gly Gly Arg Phe Leu Val Ala Val Val 
    290                 295                 300                 


Gly Ser Ala Pro Leu Ala Ala Glu Met Lys Thr Phe Met Glu Ser Val 
305                 310                 315                 320 


Leu Asp Leu Pro Leu His Asp Gly Tyr Gly Ser Thr Glu Ala Gly Ala 
                325                 330                 335     


Ser Val Leu Leu Asp Asn Gln Ile Gln Arg Pro Pro Val Leu Asp Tyr 
            340                 345                 350         


Lys Leu Val Asp Val Pro Glu Leu Gly Tyr Phe Arg Thr Asp Arg Pro 
        355                 360                 365             


His Pro Arg Gly Glu Leu Leu Leu Lys Ala Glu Thr Thr Ile Pro Gly 
    370                 375                 380                 


Tyr Tyr Lys Arg Pro Glu Val Thr Ala Glu Ile Phe Asp Glu Asp Gly 
385                 390                 395                 400 


Phe Tyr Lys Thr Gly Asp Ile Val Ala Glu Leu Glu His Asp Arg Leu 
                405                 410                 415     


Val Tyr Val Asp Arg Arg Asn Asn Val Leu Lys Leu Ser Gln Gly Glu 
            420                 425                 430         


Phe Val Thr Val Ala His Leu Glu Ala Val Phe Ala Ser Ser Pro Leu 
        435                 440                 445             


Ile Arg Gln Ile Phe Ile Tyr 
    450                 455 


<210>  3
<211>  33
<212>  PRT
<213>  Nocardia iowensis

<400>  3

Val Ser Arg Ala Ala Lys Ala Met Leu Gly Val Ala Ser Ala Asp Met 
1               5                   10                  15      


Arg Pro Asp Ala His Phe Thr Asp Leu Gly Gly Asp Ser Leu Ser Ala 
            20                  25                  30          


Leu 
    


<210>  4
<211>  47
<212>  PRT
<213>  Nocardia iowensis

<400>  4

Leu Glu Thr Val Ser Arg Ala Ala Lys Ala Met Leu Gly Val Ala Ser 
1               5                   10                  15      


Ala Asp Met Arg Pro Asp Ala His Phe Thr Asp Leu Gly Gly Asp Ser 
            20                  25                  30          


Leu Ser Ala Leu Ser Phe Ser Asn Leu Leu His Glu Ile Phe Gly 
        35                  40                  45          


<210>  5
<211>  292
<212>  PRT
<213>  Nocardia iowensis

<400>  5

Tyr Leu Gly Arg Phe Leu Cys Leu Glu Trp Leu Glu Arg Leu Asp Lys 
1               5                   10                  15      


Thr Gly Gly Thr Leu Ile Cys Val Val Arg Gly Ser Asp Ala Ala Ala 
            20                  25                  30          


Ala Arg Lys Arg Leu Asp Ser Ala Phe Asp Ser Gly Asp Pro Gly Leu 
        35                  40                  45              


Leu Glu His Tyr Gln Gln Leu Ala Ala Arg Thr Leu Glu Val Leu Ala 
    50                  55                  60                  


Gly Asp Ile Gly Asp Pro Asn Leu Gly Leu Asp Asp Ala Thr Trp Gln 
65                  70                  75                  80  


Arg Leu Ala Glu Thr Val Asp Leu Ile Val His Pro Ala Ala Leu Val 
                85                  90                  95      


Asn His Val Leu Pro Tyr Thr Gln Leu Phe Gly Pro Asn Val Val Gly 
            100                 105                 110         


Thr Ala Glu Ile Val Arg Leu Ala Ile Thr Ala Arg Arg Lys Pro Val 
        115                 120                 125             


Thr Tyr Leu Ser Thr Val Gly Val Ala Asp Gln Val Asp Pro Ala Glu 
    130                 135                 140                 


Tyr Gln Glu Asp Ser Asp Val Arg Glu Met Ser Ala Val Arg Val Val 
145                 150                 155                 160 


Arg Glu Ser Tyr Ala Asn Gly Tyr Gly Asn Ser Lys Trp Ala Gly Glu 
                165                 170                 175     


Val Leu Leu Arg Glu Ala His Asp Leu Cys Gly Leu Pro Val Ala Val 
            180                 185                 190         


Phe Arg Ser Asp Met Ile Leu Ala His Ser Arg Tyr Ala Gly Gln Leu 
        195                 200                 205             


Asn Val Gln Asp Val Phe Thr Arg Leu Ile Leu Ser Leu Val Ala Thr 
    210                 215                 220                 


Gly Ile Ala Pro Tyr Ser Phe Tyr Arg Thr Asp Ala Asp Gly Asn Arg 
225                 230                 235                 240 


Gln Arg Ala His Tyr Asp Gly Leu Pro Ala Asp Phe Thr Ala Ala Ala 
                245                 250                 255     


Ile Thr Ala Leu Gly Ile Gln Ala Thr Glu Gly Phe Arg Thr Tyr Asp 
            260                 265                 270         


Val Leu Asn Pro Tyr Asp Asp Gly Ile Ser Leu Asp Glu Phe Val Asp 
        275                 280                 285             


Trp Leu Val Glu 
    290         


<210>  6
<211>  345
<212>  PRT
<213>  Nocardia iowensis

<400>  6

Arg Ala Ala Asp Leu Thr Leu Asp Lys Phe Ile Asp Ala Arg Thr Leu 
1               5                   10                  15      


Ala Ala Ala Asp Ser Ile Pro His Ala Pro Val Pro Ala Gln Thr Val 
            20                  25                  30          


Leu Leu Thr Gly Ala Asn Gly Tyr Leu Gly Arg Phe Leu Cys Leu Glu 
        35                  40                  45              


Trp Leu Glu Arg Leu Asp Lys Thr Gly Gly Thr Leu Ile Cys Val Val 
    50                  55                  60                  


Arg Gly Ser Asp Ala Ala Ala Ala Arg Lys Arg Leu Asp Ser Ala Phe 
65                  70                  75                  80  


Asp Ser Gly Asp Pro Gly Leu Leu Glu His Tyr Gln Gln Leu Ala Ala 
                85                  90                  95      


Arg Thr Leu Glu Val Leu Ala Gly Asp Ile Gly Asp Pro Asn Leu Gly 
            100                 105                 110         


Leu Asp Asp Ala Thr Trp Gln Arg Leu Ala Glu Thr Val Asp Leu Ile 
        115                 120                 125             


Val His Pro Ala Ala Leu Val Asn His Val Leu Pro Tyr Thr Gln Leu 
    130                 135                 140                 


Phe Gly Pro Asn Val Val Gly Thr Ala Glu Ile Val Arg Leu Ala Ile 
145                 150                 155                 160 


Thr Ala Arg Arg Lys Pro Val Thr Tyr Leu Ser Thr Val Gly Val Ala 
                165                 170                 175     


Asp Gln Val Asp Pro Ala Glu Tyr Gln Glu Asp Ser Asp Val Arg Glu 
            180                 185                 190         


Met Ser Ala Val Arg Val Val Arg Glu Ser Tyr Ala Asn Gly Tyr Gly 
        195                 200                 205             


Asn Ser Lys Trp Ala Gly Glu Val Leu Leu Arg Glu Ala His Asp Leu 
    210                 215                 220                 


Cys Gly Leu Pro Val Ala Val Phe Arg Ser Asp Met Ile Leu Ala His 
225                 230                 235                 240 


Ser Arg Tyr Ala Gly Gln Leu Asn Val Gln Asp Val Phe Thr Arg Leu 
                245                 250                 255     


Ile Leu Ser Leu Val Ala Thr Gly Ile Ala Pro Tyr Ser Phe Tyr Arg 
            260                 265                 270         


Thr Asp Ala Asp Gly Asn Arg Gln Arg Ala His Tyr Asp Gly Leu Pro 
        275                 280                 285             


Ala Asp Phe Thr Ala Ala Ala Ile Thr Ala Leu Gly Ile Gln Ala Thr 
    290                 295                 300                 


Glu Gly Phe Arg Thr Tyr Asp Val Leu Asn Pro Tyr Asp Asp Gly Ile 
305                 310                 315                 320 


Ser Leu Asp Glu Phe Val Asp Trp Leu Val Glu Ser Gly His Pro Ile 
                325                 330                 335     


Gln Arg Ile Thr Asp Tyr Ser Asp Trp 
            340                 345 


<210>  7
<211>  1174
<212>  PRT
<213>  Nocardia iowensis

<400>  7

Met Ala Val Asp Ser Pro Asp Glu Arg Leu Gln Arg Arg Ile Ala Gln 
1               5                   10                  15      


Leu Phe Ala Glu Asp Glu Gln Val Lys Ala Ala Arg Pro Leu Glu Ala 
            20                  25                  30          


Val Ser Ala Ala Val Ser Ala Pro Gly Met Arg Leu Ala Gln Ile Ala 
        35                  40                  45              


Ala Thr Val Met Ala Gly Tyr Ala Asp Arg Pro Ala Ala Gly Gln Arg 
    50                  55                  60                  


Ala Phe Glu Leu Asn Thr Asp Asp Ala Thr Gly Arg Thr Ser Leu Arg 
65                  70                  75                  80  


Leu Leu Pro Arg Phe Glu Thr Ile Thr Tyr Arg Glu Leu Trp Gln Arg 
                85                  90                  95      


Val Gly Glu Val Ala Ala Ala Trp His His Asp Pro Glu Asn Pro Leu 
            100                 105                 110         


Arg Ala Gly Asp Phe Val Ala Leu Leu Gly Phe Thr Ser Ile Asp Tyr 
        115                 120                 125             


Ala Thr Leu Asp Leu Ala Asp Ile His Leu Gly Ala Val Thr Val Pro 
    130                 135                 140                 


Leu Gln Ala Ser Ala Ala Val Ser Gln Leu Ile Ala Ile Leu Thr Glu 
145                 150                 155                 160 


Thr Ser Pro Arg Leu Leu Ala Ser Thr Pro Glu His Leu Asp Ala Ala 
                165                 170                 175     


Val Glu Cys Leu Leu Ala Gly Thr Thr Pro Glu Arg Leu Val Val Phe 
            180                 185                 190         


Asp Tyr His Pro Glu Asp Asp Asp Gln Arg Ala Ala Phe Glu Ser Ala 
        195                 200                 205             


Arg Arg Arg Leu Ala Asp Ala Gly Ser Leu Val Ile Val Glu Thr Leu 
    210                 215                 220                 


Asp Ala Val Arg Ala Arg Gly Arg Asp Leu Pro Ala Ala Pro Leu Phe 
225                 230                 235                 240 


Val Pro Asp Thr Asp Asp Asp Pro Leu Ala Leu Leu Ile Tyr Thr Ser 
                245                 250                 255     


Gly Ser Thr Gly Thr Pro Lys Gly Ala Met Tyr Thr Asn Arg Leu Ala 
            260                 265                 270         


Ala Thr Met Trp Gln Gly Asn Ser Met Leu Gln Gly Asn Ser Gln Arg 
        275                 280                 285             


Val Gly Ile Asn Leu Asn Tyr Met Pro Met Ser His Ile Ala Gly Arg 
    290                 295                 300                 


Ile Ser Leu Phe Gly Val Leu Ala Arg Gly Gly Thr Ala Tyr Phe Ala 
305                 310                 315                 320 


Ala Lys Ser Asp Met Ser Thr Leu Phe Glu Asp Ile Gly Leu Val Arg 
                325                 330                 335     


Pro Thr Glu Ile Phe Phe Val Pro Arg Val Cys Asp Met Val Phe Gln 
            340                 345                 350         


Arg Tyr Gln Ser Glu Leu Asp Arg Arg Ser Val Ala Gly Ala Asp Leu 
        355                 360                 365             


Asp Thr Leu Asp Arg Glu Val Lys Ala Asp Leu Arg Gln Asn Tyr Leu 
    370                 375                 380                 


Gly Gly Arg Phe Leu Val Ala Val Val Gly Ser Ala Pro Leu Ala Ala 
385                 390                 395                 400 


Glu Met Lys Thr Phe Met Glu Ser Val Leu Asp Leu Pro Leu His Asp 
                405                 410                 415     


Gly Tyr Gly Ser Thr Glu Ala Gly Ala Ser Val Leu Leu Asp Asn Gln 
            420                 425                 430         


Ile Gln Arg Pro Pro Val Leu Asp Tyr Lys Leu Val Asp Val Pro Glu 
        435                 440                 445             


Leu Gly Tyr Phe Arg Thr Asp Arg Pro His Pro Arg Gly Glu Leu Leu 
    450                 455                 460                 


Leu Lys Ala Glu Thr Thr Ile Pro Gly Tyr Tyr Lys Arg Pro Glu Val 
465                 470                 475                 480 


Thr Ala Glu Ile Phe Asp Glu Asp Gly Phe Tyr Lys Thr Gly Asp Ile 
                485                 490                 495     


Val Ala Glu Leu Glu His Asp Arg Leu Val Tyr Val Asp Arg Arg Asn 
            500                 505                 510         


Asn Val Leu Lys Leu Ser Gln Gly Glu Phe Val Thr Val Ala His Leu 
        515                 520                 525             


Glu Ala Val Phe Ala Ser Ser Pro Leu Ile Arg Gln Ile Phe Ile Tyr 
    530                 535                 540                 


Gly Ser Ser Glu Arg Ser Tyr Leu Leu Ala Val Ile Val Pro Thr Asp 
545                 550                 555                 560 


Asp Ala Leu Arg Gly Arg Asp Thr Ala Thr Leu Lys Ser Ala Leu Ala 
                565                 570                 575     


Glu Ser Ile Gln Arg Ile Ala Lys Asp Ala Asn Leu Gln Pro Tyr Glu 
            580                 585                 590         


Ile Pro Arg Asp Phe Leu Ile Glu Thr Glu Pro Phe Thr Ile Ala Asn 
        595                 600                 605             


Gly Leu Leu Ser Gly Ile Ala Lys Leu Leu Arg Pro Asn Leu Lys Glu 
    610                 615                 620                 


Arg Tyr Gly Ala Gln Leu Glu Gln Met Tyr Thr Asp Leu Ala Thr Gly 
625                 630                 635                 640 


Gln Ala Asp Glu Leu Leu Ala Leu Arg Arg Glu Ala Ala Asp Leu Pro 
                645                 650                 655     


Val Leu Glu Thr Val Ser Arg Ala Ala Lys Ala Met Leu Gly Val Ala 
            660                 665                 670         


Ser Ala Asp Met Arg Pro Asp Ala His Phe Thr Asp Leu Gly Gly Asp 
        675                 680                 685             


Ser Leu Ser Ala Leu Ser Phe Ser Asn Leu Leu His Glu Ile Phe Gly 
    690                 695                 700                 


Val Glu Val Pro Val Gly Val Val Val Ser Pro Ala Asn Glu Leu Arg 
705                 710                 715                 720 


Asp Leu Ala Asn Tyr Ile Glu Ala Glu Arg Asn Ser Gly Ala Lys Arg 
                725                 730                 735     


Pro Thr Phe Thr Ser Val His Gly Gly Gly Ser Glu Ile Arg Ala Ala 
            740                 745                 750         


Asp Leu Thr Leu Asp Lys Phe Ile Asp Ala Arg Thr Leu Ala Ala Ala 
        755                 760                 765             


Asp Ser Ile Pro His Ala Pro Val Pro Ala Gln Thr Val Leu Leu Thr 
    770                 775                 780                 


Gly Ala Asn Gly Tyr Leu Gly Arg Phe Leu Cys Leu Glu Trp Leu Glu 
785                 790                 795                 800 


Arg Leu Asp Lys Thr Gly Gly Thr Leu Ile Cys Val Val Arg Gly Ser 
                805                 810                 815     


Asp Ala Ala Ala Ala Arg Lys Arg Leu Asp Ser Ala Phe Asp Ser Gly 
            820                 825                 830         


Asp Pro Gly Leu Leu Glu His Tyr Gln Gln Leu Ala Ala Arg Thr Leu 
        835                 840                 845             


Glu Val Leu Ala Gly Asp Ile Gly Asp Pro Asn Leu Gly Leu Asp Asp 
    850                 855                 860                 


Ala Thr Trp Gln Arg Leu Ala Glu Thr Val Asp Leu Ile Val His Pro 
865                 870                 875                 880 


Ala Ala Leu Val Asn His Val Leu Pro Tyr Thr Gln Leu Phe Gly Pro 
                885                 890                 895     


Asn Val Val Gly Thr Ala Glu Ile Val Arg Leu Ala Ile Thr Ala Arg 
            900                 905                 910         


Arg Lys Pro Val Thr Tyr Leu Ser Thr Val Gly Val Ala Asp Gln Val 
        915                 920                 925             


Asp Pro Ala Glu Tyr Gln Glu Asp Ser Asp Val Arg Glu Met Ser Ala 
    930                 935                 940                 


Val Arg Val Val Arg Glu Ser Tyr Ala Asn Gly Tyr Gly Asn Ser Lys 
945                 950                 955                 960 


Trp Ala Gly Glu Val Leu Leu Arg Glu Ala His Asp Leu Cys Gly Leu 
                965                 970                 975     


Pro Val Ala Val Phe Arg Ser Asp Met Ile Leu Ala His Ser Arg Tyr 
            980                 985                 990         


Ala Gly Gln Leu Asn Val Gln Asp  Val Phe Thr Arg Leu  Ile Leu Ser 
        995                 1000                 1005             


Leu Val  Ala Thr Gly Ile Ala  Pro Tyr Ser Phe Tyr  Arg Thr Asp 
    1010                 1015                 1020             


Ala Asp  Gly Asn Arg Gln Arg  Ala His Tyr Asp Gly  Leu Pro Ala 
    1025                 1030                 1035             


Asp Phe  Thr Ala Ala Ala Ile  Thr Ala Leu Gly Ile  Gln Ala Thr 
    1040                 1045                 1050             


Glu Gly  Phe Arg Thr Tyr Asp  Val Leu Asn Pro Tyr  Asp Asp Gly 
    1055                 1060                 1065             


Ile Ser  Leu Asp Glu Phe Val  Asp Trp Leu Val Glu  Ser Gly His 
    1070                 1075                 1080             


Pro Ile  Gln Arg Ile Thr Asp  Tyr Ser Asp Trp Phe  His Arg Phe 
    1085                 1090                 1095             


Glu Thr  Ala Ile Arg Ala Leu  Pro Glu Lys Gln Arg  Gln Ala Ser 
    1100                 1105                 1110             


Val Leu  Pro Leu Leu Asp Ala  Tyr Arg Asn Pro Cys  Pro Ala Val 
    1115                 1120                 1125             


Arg Gly  Ala Ile Leu Pro Ala  Lys Glu Phe Gln Ala  Ala Val Gln 
    1130                 1135                 1140             


Thr Ala  Lys Ile Gly Pro Glu  Gln Asp Ile Pro His  Leu Ser Ala 
    1145                 1150                 1155             


Pro Leu  Ile Asp Lys Tyr Val  Ser Asp Leu Glu Leu  Leu Gln Leu 
    1160                 1165                 1170             


Leu 
    


<210>  8
<211>  4690
<212>  DNA
<213>  Nocardia iowensis

<400>  8
acggcgtagg cgatcacgta accggccacg atcagctgca aggcagcgcc ggaggtgtgc       60

agggaatcgc gaatcgacgg aatcgcgaca ttgacgatgg aggagtcgag caccgccatg      120

aactggccgg tgaggatcac cgtgaggatg gccagggtgc gccgcgcacc gtacgcggcc      180

ggtggagctg ccgggggcgc gacaaccggc gatgcggtgg ccgggtccgg cgacaagatc      240

tgggtcatat gctgatcttg cgagctcgat gacaccggta acaggagccc attaaaactg      300

gtaccggcaa tacctggata agcggtcgga tcctgggccg ctgcggtgga gtggccgccg      360

ttccggcccg atgtggccaa gaccactcga gtcaccgccg cgtatcacct tcccggaagt      420

atttacttag gctaacgtgt tttacgggtt gcagggcttt tcctacttat gacaagggag      480

gcttgccatg gcagtggatt caccggatga gcggctacag cgccgcattg cacagttgtt      540

tgcagaagat gagcaggtca aggccgcacg tccgctcgaa gcggtgagcg cggcggtgag      600

cgcgcccggt atgcggctgg cgcagatcgc cgccactgtt atggcgggtt acgccgaccg      660

cccggccgcc gggcagcgtg cgttcgaact gaacaccgac gacgcgacgg gccgcacctc      720

gctgcggtta cttccccgat tcgagaccat cacctatcgc gaactgtggc agcgagtcgg      780

cgaggttgcc gcggcctggc atcatgatcc cgagaacccc ttgcgcgcag gtgatttcgt      840

cgccctgctc ggcttcacca gcatcgacta cgccaccctc gacctggccg atatccacct      900

cggcgcggtt accgtgccgt tgcaggccag cgcggcggtg tcccagctga tcgctatcct      960

caccgagact tcgccgcggc tgctcgcctc gaccccggag cacctcgatg cggcggtcga     1020

gtgcctactc gcgggcacca caccggaacg actggtggtc ttcgactacc accccgagga     1080

cgacgaccag cgtgcggcct tcgaatccgc ccgccgccgc cttgccgacg cgggcagctt     1140

ggtgatcgtc gaaacgctcg atgccgtgcg tgcccggggc cgcgacttac cggccgcgcc     1200

actgttcgtt cccgacaccg acgacgaccc gctggccctg ctgatctaca cctccggcag     1260

caccggaacg ccgaagggcg cgatgtacac caatcggttg gccgccacga tgtggcaggg     1320

gaactcgatg ctgcagggga actcgcaacg ggtcgggatc aatctcaact acatgccgat     1380

gagccacatc gccggtcgca tatcgctgtt cggcgtgctc gctcgcggtg gcaccgcata     1440

cttcgcggcc aagagcgaca tgtcgacact gttcgaagac atcggcttgg tacgtcccac     1500

cgagatcttc ttcgtcccgc gcgtgtgcga catggtcttc cagcgctatc agagcgagct     1560

ggaccggcgc tcggtggcgg gcgccgacct ggacacgctc gatcgggaag tgaaagccga     1620

cctccggcag aactacctcg gtgggcgctt cctggtggcg gtcgtcggca gcgcgccgct     1680

ggccgcggag atgaagacgt tcatggagtc cgtcctcgat ctgccactgc acgacgggta     1740

cgggtcgacc gaggcgggcg caagcgtgct gctcgacaac cagatccagc ggccgccggt     1800

gctcgattac aagctcgtcg acgtgcccga actgggttac ttccgcaccg accggccgca     1860

tccgcgcggt gagctgttgt tgaaggcgga gaccacgatt ccgggctact acaagcggcc     1920

cgaggtcacc gcggagatct tcgacgagga cggcttctac aagaccggcg atatcgtggc     1980

cgagctcgag cacgatcggc tggtctatgt cgaccgtcgc aacaatgtgc tcaaactgtc     2040

gcagggcgag ttcgtgaccg tcgcccatct cgaggccgtg ttcgccagca gcccgctgat     2100

ccggcagatc ttcatctacg gcagcagcga acgttcctat ctgctcgcgg tgatcgtccc     2160

caccgacgac gcgctgcgcg gccgcgacac cgccaccttg aaatcggcac tggccgaatc     2220

gattcagcgc atcgccaagg acgcgaacct gcagccctac gagattccgc gcgatttcct     2280

gatcgagacc gagccgttca ccatcgccaa cggactgctc tccggcatcg cgaagctgct     2340

gcgccccaat ctgaaggaac gctacggcgc tcagctggag cagatgtaca ccgatctcgc     2400

gacaggccag gccgatgagc tgctcgccct gcgccgcgaa gccgccgacc tgccggtgct     2460

cgaaaccgtc agccgggcag cgaaagcgat gctcggcgtc gcctccgccg atatgcgtcc     2520

cgacgcgcac ttcaccgacc tgggcggcga ttccctttcc gcgctgtcgt tctcgaacct     2580

gctgcacgag atcttcgggg tcgaggtgcc ggtgggtgtc gtcgtcagcc cggcgaacga     2640

gctgcgcgat ctggcgaatt acattgaggc ggaacgcaac tcgggcgcga agcgtcccac     2700

cttcacctcg gtgcacggcg gcggttccga gatccgcgcc gccgatctga ccctcgacaa     2760

gttcatcgat gcccgcaccc tggccgccgc cgacagcatt ccgcacgcgc cggtgccagc     2820

gcagacggtg ctgctgaccg gcgcgaacgg ctacctcggc cggttcctgt gcctggaatg     2880

gctggagcgg ctggacaaga cgggtggcac gctgatctgc gtcgtgcgcg gtagtgacgc     2940

ggccgcggcc cgtaaacggc tggactcggc gttcgacagc ggcgatcccg gcctgctcga     3000

gcactaccag caactggccg cacggaccct ggaagtcctc gccggtgata tcggcgaccc     3060

gaatctcggt ctggacgacg cgacttggca gcggttggcc gaaaccgtcg acctgatcgt     3120

ccatcccgcc gcgttggtca accacgtcct tccctacacc cagctgttcg gccccaatgt     3180

cgtcggcacc gccgaaatcg tccggttggc gatcacggcg cggcgcaagc cggtcaccta     3240

cctgtcgacc gtcggagtgg ccgaccaggt cgacccggcg gagtatcagg aggacagcga     3300

cgtccgcgag atgagcgcgg tgcgcgtcgt gcgcgagagt tacgccaacg gctacggcaa     3360

cagcaagtgg gcgggggagg tcctgctgcg cgaagcacac gatctgtgtg gcttgccggt     3420

cgcggtgttc cgttcggaca tgatcctggc gcacagccgg tacgcgggtc agctcaacgt     3480

ccaggacgtg ttcacccggc tgatcctcag cctggtcgcc accggcatcg cgccgtactc     3540

gttctaccga accgacgcgg acggcaaccg gcagcgggcc cactatgacg gcttgccggc     3600

ggacttcacg gcggcggcga tcaccgcgct cggcatccaa gccaccgaag gcttccggac     3660

ctacgacgtg ctcaatccgt acgacgatgg catctccctc gatgaattcg tcgactggct     3720

cgtcgaatcc ggccacccga tccagcgcat caccgactac agcgactggt tccaccgttt     3780

cgagacggcg atccgcgcgc tgccggaaaa gcaacgccag gcctcggtgc tgccgttgct     3840

ggacgcctac cgcaacccct gcccggcggt ccgcggcgcg atactcccgg ccaaggagtt     3900

ccaagcggcg gtgcaaacag ccaaaatcgg tccggaacag gacatcccgc atttgtccgc     3960

gccactgatc gataagtacg tcagcgatct ggaactgctt cagctgctct gacggatatc     4020

aggccgccgc gcgcacctcg tcggtgcgtt cggcgccttc gcgccggagg cgaaacagga     4080

ataccgccga gccacccagg acagcggcgt agacgatgac gaagctgttg atcaggacct     4140

gggcgaccgg ccaccacggc gggaacagga acagcccgac gacaacgtag tccgggctgt     4200

attcccacgt ccacgcgccg atcgagacga agagcgcggc cgaggcaagc caccaccacg     4260

gctgcgactg cgccctgtgc agtagataga cgaacagggg aacgaaccac acccagtggt     4320

ggtcccagga gaacggcgag accgcgcagg cggtgaggcc ggcgagggtg accgcgagga     4380

gctgttcgcc acgccgatac aggccgatgg tgacggccag actcgccagc gcgacggagc     4440

ccgcgatgag cagccacagc cacaccggcg ccgggtgatg ggtcaggtgc gcgatggcgc     4500

cgcggatgga ttgattggac gggtgcatat cgtccgcgat ccgattggac tggaagaacg     4560

tcgaggtcca gtactgccgg gaatcggcgg gcagcacgat ccaggcgagg acgatggacg     4620

cgatgaacac cgccacggcg gtgcacgcgg accgccactg ccgcaacgcg aggaattgca     4680

cgacgaagta                                                            4690


<210>  9
<211>  3525
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nocardia sp. NRRL 5646 ATP/NADPH-dependent carboxylic acid 
       reductase (car), EMBL Nucleotide Sequence Database accession no. 
       AY495697, codon optimized for E.coli

<400>  9
atggcagtcg acagcccaga cgaacgcctg caaagacgca tcgcccaatt gttcgcagag       60

gacgagcagg tgaaggcggc tcgcccgctg gaagccgtga gcgcggctgt ttctgctccg      120

ggtatgcgcc tggcgcagat cgcagcgacg gtcatggccg gctatgccga ccgtcctgca      180

gccggtcaac gcgcattcga gctgaacact gatgacgcca ccggtcgcac cagcttgcgc      240

ctgctgccgc gttttgaaac catcacctat cgtgagctgt ggcaacgcgt gggcgaggtg      300

gcggctgcgt ggcatcatga cccggaaaac ccgttgcgtg cgggcgattt cgtggcactg      360

ctgggtttca ccagcattga ttatgcgacg ctggacctgg ccgacatcca tctgggtgct      420

gtgaccgtgc cgctgcaggc cagcgcagca gtgtcccaac tgattgcgat cctgacggaa      480

acctcccctc gcttgctggc gtctaccccg gagcacctgg acgcagcggt tgaatgtctg      540

ctggcgggta ccaccccgga gcgcctggtc gtgtttgatt atcacccgga agatgacgat      600

caacgcgcag cgtttgagag cgcgcgtcgc cgtctggctg atgcgggtag cctggttatc      660

gttgaaaccc tggacgcggt ccgtgcacgt ggccgtgacc tgccagcggc accgctgttc      720

gtcccggaca ctgacgatga tccactggcg ctgctgattt acaccagcgg cagcaccggt      780

acgccgaagg gcgcgatgta caccaatcgc ttggcggcga ccatgtggca aggtaacagc      840

atgctgcaag gtaactccca gcgtgttggc atcaatttga attacatgcc gatgagccat      900

attgcgggtc gtattagcct gttcggtgtg ctggctcgtg gcggtacggc ctatttcgca      960

gcgaagtccg atatgagcac cctgtttgaa gatatcggtc tggtccgtcc gaccgagatc     1020

ttcttcgttc cgcgtgtttg tgatatggtg tttcagcgtt atcagtcgga actggaccgt     1080

cgtagcgtcg caggtgcgga cctggacacg ctggatcgcg aggtcaaagc cgacctgcgc     1140

cagaattatc tgggcggtcg cttcctggtc gccgtggtgg gctccgctcc gctggcagcg     1200

gagatgaaaa cctttatgga gagcgtcttg gatctgccgc tgcacgatgg ctatggtagc     1260

acggaagcgg gtgcgtccgt cttgctggat aaccaaattc agcgtcctcc agtgctggac     1320

tataaactgg tggatgtgcc ggaactgggc tattttcgta ccgatcgccc gcatccgcgt     1380

ggtgagctgc tgctgaaggc ggaaactacc atcccgggtt actacaagcg tccggaggtt     1440

acggcagaaa tctttgatga agatggtttc tacaagacgg gtgacatcgt tgcggagctg     1500

gaacacgatc gtttggttta cgttgaccgt cgcaacaacg tgctgaaact gtctcaaggt     1560

gagttcgtca ctgttgctca cctggaagct gtgtttgcaa gcagcccgct gattcgtcag     1620

atcttcatct acggtagctc tgaacgcagc tacttgctgg ccgttatcgt tccgaccgac     1680

gacgcattgc gtggccgcga cactgcaacc ctgaagagcg cattggccga gagcatccaa     1740

cgtattgcaa aagacgcgaa cctgcaacca tacgagatcc cgcgtgattt cctgatcgaa     1800

accgaaccgt tcaccattgc gaatggtctg ctgagcggca ttgcaaagct gctgcgtccg     1860

aatctgaaag agcgttacgg tgcccagttg gagcagatgt acacggactt ggcgacgggt     1920

caggcggacg aattgctggc gctgcgtcgt gaggcggcag acctgccggt actggaaacc     1980

gttagccgtg cggccaaagc gatgctgggt gttgccagcg cggatatgcg tccggatgcg     2040

cactttaccg acttgggcgg tgacagcctg agcgcgctga gcttctctaa tctgctgcac     2100

gaaatctttg gcgtggaggt gccggttggc gtcgtggtga gccctgcaaa tgaattgcgt     2160

gatctggcta attacatcga ggccgaacgc aatagcggtg cgaaacgtcc gacctttacc     2220

agcgtgcatg gcggtggctc ggagattcgt gcggcggatc tgacgctgga taagtttatt     2280

gatgctcgta cgctggcagc cgctgacagc attccgcacg caccggtgcc agcgcagacg     2340

gtcctgctga cgggtgccaa cggttacctg ggtcgctttc tgtgcctgga gtggctggag     2400

cgtctggaca aaacgggcgg taccctgatc tgtgttgtgc gtggctcgga tgcagcagcc     2460

gcacgcaaac gtttggacag cgccttcgac tcgggtgacc cgggtctgtt ggagcattat     2520

cagcagctgg cggcacgcac cctggaggtt ctggcgggtg atattggcga tccgaatctg     2580

ggcctggacg atgcgacctg gcagcgtctg gcggagactg tcgatttgat tgtccacccg     2640

gcagcgttgg ttaaccatgt tctgccgtac acccaactgt tcggcccgaa cgttgtaggt     2700

acggccgaaa ttgtccgtct ggcaatcact gcgcgtcgca agccggtcac gtacctgagc     2760

accgttggcg tggccgatca ggttgacccg gcagagtatc aagaggattc tgacgttcgt     2820

gagatgagcg cggttcgcgt tgtccgtgag agctatgcga acggttacgg caacagcaag     2880

tgggcgggtg aagttttgct gcgtgaggct cacgatttgt gcggcttgcc ggtcgcagtt     2940

ttccgttctg acatgattct ggcccacagc cgttatgcgg gtcagctgaa tgttcaggat     3000

gtgtttacgc gcctgattct gagcttggtc gcgaccggta tcgcgccgta cagcttctac     3060

cgtacggacg ccgacggtaa ccgccagcgt gcccattacg acggcctgcc ggcagacttc     3120

acggctgcgg cgattaccgc gctgggtatt caagcgacgg aaggctttcg tacctatgat     3180

gtgctgaacc cgtacgacga cggtatcagc ctggacgagt tcgtggattg gctggtggag     3240

agcggccacc cgattcagcg tattacggac tatagcgatt ggtttcaccg cttcgaaacc     3300

gccattcgtg cgctgccgga aaaacagcgt caggcgtccg tcctgccgct gctggatgca     3360

tatcgtaatc cgtgcccagc agtccgcggt gctattctgc ctgccaaaga gtttcaagcg     3420

gcggtgcaaa ccgcaaagat tggcccggag caagacatcc cgcacctgtc tgcaccgctg     3480

atcgacaaat acgttagcga tttggaactg ctgcaactgc tgtaa                     3525


<210>  10
<211>  76
<212>  PRT
<213>  Nocardia iowensis

<400>  10

Leu Pro Val Leu Glu Thr Val Ser Arg Ala Ala Lys Ala Met Leu Gly 
1               5                   10                  15      


Val Ala Ser Ala Asp Met Arg Pro Asp Ala His Phe Thr Asp Leu Gly 
            20                  25                  30          


Gly Asp Ser Leu Ser Ala Leu Ser Phe Ser Asn Leu Leu His Glu Ile 
        35                  40                  45              


Phe Gly Val Glu Val Pro Val Gly Val Val Val Ser Pro Ala Asn Glu 
    50                  55                  60                  


Leu Arg Asp Leu Ala Asn Tyr Ile Glu Ala Glu Arg 
65                  70                  75      


<210>  11
<211>  630
<212>  DNA
<213>  Escherichia coli

<400>  11
ttaatcgtgt tggcacagcg ttatgactat cttttctttt atctgccagt gcacagcaaa       60

catctcattc tcacgatgaa tgatgacctg ctgtttattc cagctaatta tctgatagtc      120

cagaaaacct gcatcagttt ggatctcact tgccttaaat gcgctctctt tggcggaaaa      180

tgccagtgtc agcgccagag aaaaggctaa accgcagtct gcgagtcgct cgtgttccgc      240

tggtgtaata atgttgtctg tcaattctct tgcggtttgt acagaaaaaa tttcttctat      300

atcaatgcca atcggttgac gagataccac ggctaatgcc gtagtcccac agtggctaat      360

actgccgtat acctccgcag gccagacagg ttggcgtagc tcgccgattg cgggcacaca      420

tttatagcca tattcccgca aagcataaac agcagcgatc cgtccggcta aatgctctgt      480

tttacgttta cgtccagcgt gttgcagttg tgcgtagtgc ggcagccaga gtaaatcctg      540

ctcacaaaaa ttcgccggat cgaactcaac aaaatgcagc gtatgtccgg caaaggggag      600

ggaggtatgc gtagttttca tatcgaccat                                       630


<210>  12
<211>  209
<212>  PRT
<213>  Escherichia coli

<400>  12

Met Val Asp Met Lys Thr Thr His Thr Ser Leu Pro Phe Ala Gly His 
1               5                   10                  15      


Thr Leu His Phe Val Glu Phe Asp Pro Ala Asn Phe Cys Glu Gln Asp 
            20                  25                  30          


Leu Leu Trp Leu Pro His Tyr Ala Gln Leu Gln His Ala Gly Arg Lys 
        35                  40                  45              


Arg Lys Thr Glu His Leu Ala Gly Arg Ile Ala Ala Val Tyr Ala Leu 
    50                  55                  60                  


Arg Glu Tyr Gly Tyr Lys Cys Val Pro Ala Ile Gly Glu Leu Arg Gln 
65                  70                  75                  80  


Pro Val Trp Pro Ala Glu Val Tyr Gly Ser Ile Ser His Cys Gly Thr 
                85                  90                  95      


Thr Ala Leu Ala Val Val Ser Arg Gln Pro Ile Gly Ile Asp Ile Glu 
            100                 105                 110         


Glu Ile Phe Ser Val Gln Thr Ala Arg Glu Leu Thr Asp Asn Ile Ile 
        115                 120                 125             


Thr Pro Ala Glu His Glu Arg Leu Ala Asp Cys Gly Leu Ala Phe Ser 
    130                 135                 140                 


Leu Ala Leu Thr Leu Ala Phe Ser Ala Lys Glu Ser Ala Phe Lys Ala 
145                 150                 155                 160 


Ser Glu Ile Gln Thr Asp Ala Gly Phe Leu Asp Tyr Gln Ile Ile Ser 
                165                 170                 175     


Trp Asn Lys Gln Gln Val Ile Ile His Arg Glu Asn Glu Met Phe Ala 
            180                 185                 190         


Val His Trp Gln Ile Lys Glu Lys Ile Val Ile Thr Leu Cys Gln His 
        195                 200                 205             


Asp 
    


<210>  13
<211>  408
<212>  DNA
<213>  Corynebacterium glutamicum

<400>  13
ctactggata gtcaacacgc acgtcgccac cgcgtagtcg ccgtcgtggc tgatgctcaa       60

gctgctggaa aactccccga tggattcccg gaccactgcg gccaattcgg gcgccaattc      120

gatcgcgacg cgcccccacg cgtctgcgcg aacttcgatg tcccgccaca ccacggcttc      180

ttcggcgatc acgggtggct ggccataaat ggcctgtgac caggccttaa taaaggattc      240

ttttgccgcc caccgccccg ccaaatgctc ggcgtagcgg cttgcctggc gttcatttgc      300

tttacgacgc tcccctgcgg aaaacacctc catgaaggaa cttcccggct gtgcaagctg      360

ctctgcgaac gccgaaatgt gaacgaggtc ggttccaatg ctgatcat                   408


<210>  14
<211>  135
<212>  PRT
<213>  Corynebacterium glutamicum

<400>  14

Met Ile Ser Ile Gly Thr Asp Leu Val His Ile Ser Ala Phe Ala Glu 
1               5                   10                  15      


Gln Leu Ala Gln Pro Gly Ser Ser Phe Met Glu Val Phe Ser Ala Gly 
            20                  25                  30          


Glu Arg Arg Lys Ala Asn Glu Arg Gln Ala Ser Arg Tyr Ala Glu His 
        35                  40                  45              


Leu Ala Gly Arg Trp Ala Ala Lys Glu Ser Phe Ile Lys Ala Trp Ser 
    50                  55                  60                  


Gln Ala Ile Tyr Gly Gln Pro Pro Val Ile Ala Glu Glu Ala Val Val 
65                  70                  75                  80  


Trp Arg Asp Ile Glu Val Arg Ala Asp Ala Trp Gly Arg Val Ala Ile 
                85                  90                  95      


Glu Leu Ala Pro Glu Leu Ala Ala Val Val Arg Glu Ser Ile Gly Glu 
            100                 105                 110         


Phe Ser Ser Ser Leu Ser Ile Ser His Asp Gly Asp Tyr Ala Val Ala 
        115                 120                 125             


Thr Cys Val Leu Thr Ile Gln 
    130                 135 


<210>  15
<211>  7512
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gene sequence of plasmid pJexpress-404-9-CAR

<400>  15
ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc       60

ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct      120

tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa      180

ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag      240

tgtagccgta gttagcccac cacttcaaga actctgtagc accgcctaca tacctcgctc      300

tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg      360

actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca      420

cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat      480

gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg      540

tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc      600

ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc      660

ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc      720

cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg      780

cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga      840

gcgaggaagc ggaaggcgag agtagggaac tgccaggcat caaactaagc agaaggcccc      900

tgacggatgg cctttttgcg tttctacaaa ctctttctgt gttgtaaaac gacggccagt      960

cttaagctcg ggccccctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg     1020

taaaaacccg cttcggcggg tttttttatg gggggagttt agggaaagag catttgtcag     1080

aatatttaag ggcgcctgtc actttgcttg atatatgaga attatttaac cttataaatg     1140

agaaaaaagc aacgcacttt aaataagata cgttgctttt tcgattgatg aacacctata     1200

attaaactat tcatctatta tttatgattt tttgtatata caatatttct agtttgttaa     1260

agagaattaa gaaaataaat ctcgaaaata ataaagggaa aatcagtttt tgatatcaaa     1320

attatacatg tcaacgataa tacaaaatat aatacaaact ataagatgtt atcagtattt     1380

attatgcatt tagaataaat tttgtgtcgc ccttaattgt gagcggataa caattacgag     1440

cttcatgcac agtgaaatca tgaaaaattt atttgctttg tgagcggata acaattataa     1500

tatgtggaat tgtgagcgct cacaattcca caacggtttc cctctagaaa taattttgtt     1560

taacttttag gaggtaaaac atatggcagt cgacagccca gacgaacgcc tgcaaagacg     1620

catcgcccaa ttgttcgcag aggacgagca ggtgaaggcg gctcgcccgc tggaagccgt     1680

gagcgcggct gtttctgctc cgggtatgcg cctggcgcag atcgcagcga cggtcatggc     1740

cggctatgcc gaccgtcctg cagccggtca acgcgcattc gagctgaaca ctgatgacgc     1800

caccggtcgc accagcttgc gcctgctgcc gcgttttgaa accatcacct atcgtgagct     1860

gtggcaacgc gtgggcgagg tggcggctgc gtggcatcat gacccggaaa acccgttgcg     1920

tgcgggcgat ttcgtggcac tgctgggttt caccagcatt gattatgcga cgctggacct     1980

ggccgacatc catctgggtg ctgtgaccgt gccgctgcag gccagcgcag cagtgtccca     2040

actgattgcg atcctgacgg aaacctcccc tcgcttgctg gcgtctaccc cggagcacct     2100

ggacgcagcg gttgaatgtc tgctggcggg taccaccccg gagcgcctgg tcgtgtttga     2160

ttatcacccg gaagatgacg atcaacgcgc agcgtttgag agcgcgcgtc gccgtctggc     2220

tgatgcgggt agcctggtta tcgttgaaac cctggacgcg gtccgtgcac gtggccgtga     2280

cctgccagcg gcaccgctgt tcgtcccgga cactgacgat gatccactgg cgctgctgat     2340

ttacaccagc ggcagcaccg gtacgccgaa gggcgcgatg tacaccaatc gcttggcggc     2400

gaccatgtgg caaggtaaca gcatgctgca aggtaactcc cagcgtgttg gcatcaattt     2460

gaattacatg ccgatgagcc atattgcggg tcgtattagc ctgttcggtg tgctggctcg     2520

tggcggtacg gcctatttcg cagcgaagtc cgatatgagc accctgtttg aagatatcgg     2580

tctggtccgt ccgaccgaga tcttcttcgt tccgcgtgtt tgtgatatgg tgtttcagcg     2640

ttatcagtcg gaactggacc gtcgtagcgt cgcaggtgcg gacctggaca cgctggatcg     2700

cgaggtcaaa gccgacctgc gccagaatta tctgggcggt cgcttcctgg tcgccgtggt     2760

gggctccgct ccgctggcag cggagatgaa aacctttatg gagagcgtct tggatctgcc     2820

gctgcacgat ggctatggta gcacggaagc gggtgcgtcc gtcttgctgg ataaccaaat     2880

tcagcgtcct ccagtgctgg actataaact ggtggatgtg ccggaactgg gctattttcg     2940

taccgatcgc ccgcatccgc gtggtgagct gctgctgaag gcggaaacta ccatcccggg     3000

ttactacaag cgtccggagg ttacggcaga aatctttgat gaagatggtt tctacaagac     3060

gggtgacatc gttgcggagc tggaacacga tcgtttggtt tacgttgacc gtcgcaacaa     3120

cgtgctgaaa ctgtctcaag gtgagttcgt cactgttgct cacctggaag ctgtgtttgc     3180

aagcagcccg ctgattcgtc agatcttcat ctacggtagc tctgaacgca gctacttgct     3240

ggccgttatc gttccgaccg acgacgcatt gcgtggccgc gacactgcaa ccctgaagag     3300

cgcattggcc gagagcatcc aacgtattgc aaaagacgcg aacctgcaac catacgagat     3360

cccgcgtgat ttcctgatcg aaaccgaacc gttcaccatt gcgaatggtc tgctgagcgg     3420

cattgcaaag ctgctgcgtc cgaatctgaa agagcgttac ggtgcccagt tggagcagat     3480

gtacacggac ttggcgacgg gtcaggcgga cgaattgctg gcgctgcgtc gtgaggcggc     3540

agacctgccg gtactggaaa ccgttagccg tgcggccaaa gcgatgctgg gtgttgccag     3600

cgcggatatg cgtccggatg cgcactttac cgacttgggc ggtgacagcc tgagcgcgct     3660

gagcttctct aatctgctgc acgaaatctt tggcgtggag gtgccggttg gcgtcgtggt     3720

gagccctgca aatgaattgc gtgatctggc taattacatc gaggccgaac gcaatagcgg     3780

tgcgaaacgt ccgaccttta ccagcgtgca tggcggtggc tcggagattc gtgcggcgga     3840

tctgacgctg gataagttta ttgatgctcg tacgctggca gccgctgaca gcattccgca     3900

cgcaccggtg ccagcgcaga cggtcctgct gacgggtgcc aacggttacc tgggtcgctt     3960

tctgtgcctg gagtggctgg agcgtctgga caaaacgggc ggtaccctga tctgtgttgt     4020

gcgtggctcg gatgcagcag ccgcacgcaa acgtttggac agcgccttcg actcgggtga     4080

cccgggtctg ttggagcatt atcagcagct ggcggcacgc accctggagg ttctggcggg     4140

tgatattggc gatccgaatc tgggcctgga cgatgcgacc tggcagcgtc tggcggagac     4200

tgtcgatttg attgtccacc cggcagcgtt ggttaaccat gttctgccgt acacccaact     4260

gttcggcccg aacgttgtag gtacggccga aattgtccgt ctggcaatca ctgcgcgtcg     4320

caagccggtc acgtacctga gcaccgttgg cgtggccgat caggttgacc cggcagagta     4380

tcaagaggat tctgacgttc gtgagatgag cgcggttcgc gttgtccgtg agagctatgc     4440

gaacggttac ggcaacagca agtgggcggg tgaagttttg ctgcgtgagg ctcacgattt     4500

gtgcggcttg ccggtcgcag ttttccgttc tgacatgatt ctggcccaca gccgttatgc     4560

gggtcagctg aatgttcagg atgtgtttac gcgcctgatt ctgagcttgg tcgcgaccgg     4620

tatcgcgccg tacagcttct accgtacgga cgccgacggt aaccgccagc gtgcccatta     4680

cgacggcctg ccggcagact tcacggctgc ggcgattacc gcgctgggta ttcaagcgac     4740

ggaaggcttt cgtacctatg atgtgctgaa cccgtacgac gacggtatca gcctggacga     4800

gttcgtggat tggctggtgg agagcggcca cccgattcag cgtattacgg actatagcga     4860

ttggtttcac cgcttcgaaa ccgccattcg tgcgctgccg gaaaaacagc gtcaggcgtc     4920

cgtcctgccg ctgctggatg catatcgtaa tccgtgccca gcagtccgcg gtgctattct     4980

gcctgccaaa gagtttcaag cggcggtgca aaccgcaaag attggcccgg agcaagacat     5040

cccgcacctg tctgcaccgc tgatcgacaa atacgttagc gatttggaac tgctgcaact     5100

gctgtaactc gagccccaag ggcgacaccc cataattagc ccgggcgaaa ggcccagtct     5160

ttcgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg catggggagt     5220

ccccacacta ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg     5280

gaccaccgcg ctactgccgc caggcaaaca aggggtgtta tgagccatat tcaggtataa     5340

atgggctcgc gataatgttc agaattggtt aattggttgt aacactgacc cctatttgtt     5400

tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc     5460

ttcaataata ttgaaaaagg aagaatatga gtattcaaca tttccgtgtc gcccttattc     5520

ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa     5580

aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg     5640

gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag     5700

ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc     5760

gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta     5820

cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg     5880

cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca     5940

acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac     6000

caaacgacga gcgtgacacc acgatgcctg tagcgatggc aacaacgttg cgcaaactat     6060

taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg     6120

ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata     6180

aatccggagc cggtgagcgt ggttctcgcg gtatcatcgc agcgctgggg ccagatggta     6240

agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa     6300

atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaagcg gcgcgccatc     6360

gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg     6420

gtggtgaata tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat     6480

cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa     6540

gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg     6600

ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg     6660

caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg     6720

atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa     6780

cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa     6840

gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac     6900

agtattattt tctcccatga ggacggtacg cgactgggcg tggagcatct ggtcgcattg     6960

ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt     7020

ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa     7080

ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc     7140

gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt     7200

accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa     7260

gatagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg     7320

caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag     7380

ctgttgccag tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc     7440

tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa     7500

agcgggcagt ga                                                         7512


<210>  16
<211>  4566
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gene sequence of plasmid pJexpress-401-11-EcPPTase

<400>  16
ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc       60

ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct      120

tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa      180

ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag      240

tgtagccgta gttagcccac cacttcaaga actctgtagc accgcctaca tacctcgctc      300

tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg      360

actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca      420

cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat      480

gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg      540

tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc      600

ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc      660

ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc      720

cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg      780

cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga      840

gcgaggaagc ggaaggcgag agtagggaac tgccaggcat caaactaagc agaaggcccc      900

tgacggatgg cctttttgcg tttctacaaa ctctttctgt gttgtaaaac gacggccagt      960

cttaagctcg ggccccctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg     1020

taaaaacccg cttcggcggg tttttttatg gggggagttt agggaaagag catttgtcag     1080

aatatttaag ggcgcctgtc actttgcttg atatatgaga attatttaac cttataaatg     1140

agaaaaaagc aacgcacttt aaataagata cgttgctttt tcgattgatg aacacctata     1200

attaaactat tcatctatta tttatgattt tttgtatata caatatttct agtttgttaa     1260

agagaattaa gaaaataaat ctcgaaaata ataaagggaa aatcagtttt tgatatcaaa     1320

attatacatg tcaacgataa tacaaaatat aatacaaact ataagatgtt atcagtattt     1380

attatgcatt tagaataaat tttgtgtcgc ccttaattgt gagcggataa caattacgag     1440

cttcatgcac agtgaaatca tgaaaaattt atttgctttg tgagcggata acaattataa     1500

tatgtggaat tgtgagcgct cacaattcca caacggtttc cctctagaaa taattttgtt     1560

taacttttag gaggtaaaac atatggtcga tatgaaaact acgcatacct ccctcccctt     1620

tgccggacat acgctgcatt ttgttgagtt cgatccggcg aatttttgtg agcaggattt     1680

actctggctg ccgcactacg cacaactgca acacgctgga cgtaaacgta aaacagagca     1740

tttagccgga cggatcgctg ctgtttatgc tttgcgggaa tatggctata aatgtgtgcc     1800

cgcaatcggc gagctacgcc aacctgtctg gcctgcggag gtatacggca gtattagcca     1860

ctgtgggact acggcattag ccgtggtatc tcgtcaaccg attggcattg atatagaaga     1920

aattttttct gtacaaaccg caagagaatt gacagacaac attattacac cagcggaaca     1980

cgagcgactc gcagactgcg gtttagcctt ttctctggcg ctgacactgg cattttccgc     2040

caaagagagc gcatttaagg caagtgagat ccaaactgat gcaggttttc tggactatca     2100

gataattagc tggaataaac agcaggtcat cattcatcgt gagaatgaga tgtttgctgt     2160

gcactggcag ataaaagaaa agatagtcat aacgctgtgc caacacgatt aactcgagcc     2220

ccaagggcga caccccctaa ttagcccggg cgaaaggccc agtctttcga ctgagccttt     2280

cgttttattt gatgcctggc agttccctac tctcgcatgg ggagtcccca cactaccatc     2340

ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact     2400

gccgccaggc aaacaagggg tgttatgagc catattcagg tataaatggg ctcgcgataa     2460

tgttcagaat tggttaattg gttgtaacac tgacccctat ttgtttattt ttctaaatac     2520

attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa     2580

aaaggaagaa tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca     2640

tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga     2700

caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag     2760

gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta     2820

tgccacttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca     2880

ctgcgatccc cggaaaaaca gcgttccagg tattagaaga atatcctgat tcaggtgaaa     2940

atattgttga tgcgctggca gtgttcctgc gccggttgca ctcgattcct gtttgtaatt     3000

gtccttttaa cagcgatcgc gtatttcgcc tcgctcaggc gcaatcacga atgaataacg     3060

gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct     3120

ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt     3180

tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac     3240

gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt     3300

tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga     3360

ataaattgca gtttcatttg atgctcgatg agtttttcta agcggcgcgc catcgaatgg     3420

cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg     3480

aatatgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc     3540

gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa     3600

gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa     3660

cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt     3720

gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta     3780

gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc     3840

agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc     3900

tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt     3960

attttctccc atgaggacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac     4020

cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct     4080

ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac     4140

tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc     4200

actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag     4260

tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagatagc     4320

tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc     4380

agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg     4440

ccagtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc     4500

cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg     4560

cagtga                                                                4566


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 1

<400>  17
ttgtcagaat atttaagggc g                                                 21


<210>  18
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 2

<400>  18
tggtagtgtg gggactc                                                      17


<210>  19
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3

<400>  19
tatcgttgaa accctggac                                                    19


<210>  20
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 4

<400>  20
tgaaggcgga aactacc                                                      17


<210>  21
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 5

<400>  21
cacgaaatct ttggcgt                                                      17


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 6

<400>  22
gcagagtatc aagaggattc                                                   20


<210>  23
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 7

<400>  23
aatcgccatg gatgatcagc attggaaccg                                        30


<210>  24
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 8

<400>  24
aatcgaagct tctactggat agtcaacacg                                        30


