                         SEQUENCE LISTING

<110>  The University of Maryland, Baltimore
 
<120>  HIV-1 ENV-BINDING ANTIBODIES, FUSION PROTEINS, AND METHODS OF USE

<130>  61160

<150>  61/886,370
<151>  2013-10-03

<150>  61/733,172
<151>  2012-12-04

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  322
<212>  DNA
<213>  Homo sapien

<400>  1
gaaattgtgt tgacacagtc tccagccacc ctgtctgtgt ctccagggga aagagccacc       60

ctctcctgca gggccagtca gagtgttagc agcaacttag cctggtacca gcagaaacct      120

ggccaggctc ccaggctcct catctatggt gcatccacca gggccactgg tatcccagcc      180

aggttcagtg gcagtgggtc tgggacagag ttcactctca ccatcagcag cctgcagtct      240

gaagattttg cagtttatta ctgtcggcag tataataact ggcctcagac gttcggccaa      300

gggaccaaag tggatatcaa ac                                               322


<210>  2
<211>  107
<212>  PRT
<213>  Homo sapien

<400>  2

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Gly Ala Ser Thr Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Arg Gln Tyr Asn Asn Trp Pro Gln 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Asp Ile Lys 
            100                 105         


<210>  3
<211>  434
<212>  DNA
<213>  Homo sapien

<400>  3
cagctgcagc tgcaggagtc gggcccagga ctggtgaagc cttcggggac cctgtccctc       60

acctgctctg tctctggtgg ctccatgaac ggtcgaaata tctattgggc ctggatccgc      120

cagaccccag ggaaggggct ggagtggatt ggaagtgttg atgtaagtga tgaggatgcc      180

gggcgcacgt tttataagtg gcccattaag agtcgagcct ccatatccgc agacacgtcc      240

aagaatcagt tctccctgaa actgagctct gtgaccgtcg cagacacggg tgtctattac      300

tgtgcgcggg agactttata ctatgttggc aatggttatt actaccctct ggatgttttc      360

tactacaact attacatgga cgtctggggc aaggggacca cggtcagtgt ctcctcagcc      420

tccaccaagg gccc                                                        434


<210>  4
<211>  144
<212>  PRT
<213>  Homo sapien

<400>  4

Gln Leu Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gly 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ser Val Ser Gly Gly Ser Met Asn Gly Arg 
            20                  25                  30          


Asn Ile Tyr Trp Ala Trp Ile Arg Gln Thr Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Ile Gly Ser Val Asp Val Ser Asp Glu Asp Ala Gly Arg Thr Phe 
    50                  55                  60                  


Tyr Lys Trp Pro Ile Lys Ser Arg Ala Ser Ile Ser Ala Asp Thr Ser 
65                  70                  75                  80  


Lys Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Val Ala Asp Thr 
                85                  90                  95      


Gly Val Tyr Tyr Cys Ala Arg Glu Thr Leu Tyr Tyr Val Gly Asn Gly 
            100                 105                 110         


Tyr Tyr Tyr Pro Leu Asp Val Phe Tyr Tyr Asn Tyr Tyr Met Asp Val 
        115                 120                 125             


Trp Gly Lys Gly Thr Thr Val Ser Val Ser Ser Ala Ser Thr Lys Gly 
    130                 135                 140                 


<210>  5
<211>  95
<212>  PRT
<213>  Homo sapien

<400>  5

Glu Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Gly Ala Ser Thr Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Tyr Asn Asn Trp Pro 
                85                  90                  95  


<210>  6
<211>  99
<212>  PRT
<213>  Homo sapien

<400>  6

Gln Leu Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Gly Ser Ile Ser Ser Ser 
            20                  25                  30          


Ser Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu 
        35                  40                  45              


Trp Ile Gly Ser Ile Tyr Tyr Ser Gly Ser Thr Tyr Tyr Asn Pro Ser 
    50                  55                  60                  


Ile Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe 
65                  70                  75                  80  


Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Arg 
            


<210>  7
<211>  2349
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized gp120-ScFV construct comprising 
       gp120-b12-ScFV


<220>
<221>  CDS
<222>  (1)..(2349)

<400>  7
atg aga gtg acc gag atc aga aag tcc tac cag cat tgg tgg aga tgg         48
Met Arg Val Thr Glu Ile Arg Lys Ser Tyr Gln His Trp Trp Arg Trp           
1               5                   10                  15                

gga atc atg ctg ctc gga atc ctg atg atc tgc aac gcc gag gag aag         96
Gly Ile Met Leu Leu Gly Ile Leu Met Ile Cys Asn Ala Glu Glu Lys           
            20                  25                  30                    

ctg tgg gtg acc gtg tac tac ggc gtg ccc gtg tgg aag gag gcc acc        144
Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Thr           
        35                  40                  45                        

acc acc ctg ttc tgc gcc agc gac cgc aag gcc tac gac acc gag gtg        192
Thr Thr Leu Phe Cys Ala Ser Asp Arg Lys Ala Tyr Asp Thr Glu Val           
    50                  55                  60                            

cac aac gtg tgg gcc acc cac gcc tgc gtg ccc acc gac ccc aac ccc        240
His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro           
65                  70                  75                  80            

cag gag gtg gag ctg aag aac gtg acc gag aac ttc aac atg tgg aag        288
Gln Glu Val Glu Leu Lys Asn Val Thr Glu Asn Phe Asn Met Trp Lys           
                85                  90                  95                

aac aac atg gtg gag cag atg cac gag gac atc atc agc ctg tgg gac        336
Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp           
            100                 105                 110                   

cag agc ctg aag ccc tgc gtg aag ctg acc ccc ctg tgc gtg acc ctg        384
Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu           
        115                 120                 125                       

aac tgc acc gac ctg cgc aac gcc acc aac ggc aac gac acc aac acc        432
Asn Cys Thr Asp Leu Arg Asn Ala Thr Asn Gly Asn Asp Thr Asn Thr           
    130                 135                 140                           

act agt agc agc cgc ggc atg gtg ggc ggc ggc gag atg aag aac tgc        480
Thr Ser Ser Ser Arg Gly Met Val Gly Gly Gly Glu Met Lys Asn Cys           
145                 150                 155                 160           

agc ttc aac atc acc acc aac atc cgc ggc aag gtg cag aag gag tac        528
Ser Phe Asn Ile Thr Thr Asn Ile Arg Gly Lys Val Gln Lys Glu Tyr           
                165                 170                 175               

gcc ctg ttc tac aag ctg gac atc gcc ccc atc gac aac aac agc aac        576
Ala Leu Phe Tyr Lys Leu Asp Ile Ala Pro Ile Asp Asn Asn Ser Asn           
            180                 185                 190                   

aac cgc tac cgc ctg atc agc tgc aac acc agc gtg atc acc cag gcc        624
Asn Arg Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln Ala           
        195                 200                 205                       

tgc ccc aag gtg agc ttc gag ccc atc ccc atc cac tac tgc gcc ccc        672
Cys Pro Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro           
    210                 215                 220                           

gcc ggc ttc gcc atc ctg aag tgc aag gac aag aag ttc aac ggc aag        720
Ala Gly Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Lys           
225                 230                 235                 240           

ggc ccc tgc acc aac gtg agc acc gtg cag tgc acc cac ggc atc cgc        768
Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg           
                245                 250                 255               

ccc gtg gtg agc acc cag ctg ctg ctg aac ggc agc ctg gcc gag gag        816
Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu           
            260                 265                 270                   

gag gtg gtg atc cgc agc gcc aac ttc gcc gac aac gcc aag gtg atc        864
Glu Val Val Ile Arg Ser Ala Asn Phe Ala Asp Asn Ala Lys Val Ile           
        275                 280                 285                       

atc gtg cag ctg aac gag agc gtg gag atc aac tgc acc cgc ccc aac        912
Ile Val Gln Leu Asn Glu Ser Val Glu Ile Asn Cys Thr Arg Pro Asn           
    290                 295                 300                           

aac aac acc cgc aag tcc atc cac atc ggc ccc ggc cgc gcc ttc tac        960
Asn Asn Thr Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr           
305                 310                 315                 320           

acc acc ggc gag atc atc ggc gac atc cgc cag gcc cac tgc aac ctg       1008
Thr Thr Gly Glu Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Leu           
                325                 330                 335               

agc cgc gcc aag tgg aac gac acc ctg aac aag atc gtg atc aag ctg       1056
Ser Arg Ala Lys Trp Asn Asp Thr Leu Asn Lys Ile Val Ile Lys Leu           
            340                 345                 350                   

cgc gag cag ttc ggc aac aag acc atc gtg ttc aag cac agc agc ggc       1104
Arg Glu Gln Phe Gly Asn Lys Thr Ile Val Phe Lys His Ser Ser Gly           
        355                 360                 365                       

ggc gac ccc gag atc gtg acc cac agc ttc aat tgc ggc ggc gag ttc       1152
Gly Asp Pro Glu Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe           
    370                 375                 380                           

ttc tac tgc aac agc acc cag ctg ttc aac agc acc tgg aac gtg acc       1200
Phe Tyr Cys Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp Asn Val Thr           
385                 390                 395                 400           

gag gag agc aac aac acc gtg gag aac aac acc atc acc ctg ccc tgc       1248
Glu Glu Ser Asn Asn Thr Val Glu Asn Asn Thr Ile Thr Leu Pro Cys           
                405                 410                 415               

cgc atc aag cag atc atc aac atg tgg cag gag gtg ggc cgc gcc atg       1296
Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met           
            420                 425                 430                   

tac gcc ccc ccc atc cgc ggc cag atc cgc tgc agt tcg aac atc acc       1344
Tyr Ala Pro Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr           
        435                 440                 445                       

ggc ctg ctg ctg acc cgc gac ggc ggc ccc gag gac aac aag acc gag       1392
Gly Leu Leu Leu Thr Arg Asp Gly Gly Pro Glu Asp Asn Lys Thr Glu           
    450                 455                 460                           

gtg ttc cgc ccc ggc ggc ggc gac atg cgc gac aac tgg cgc agc gag       1440
Val Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu           
465                 470                 475                 480           

ctg tac aag tac aag gtg gtg aag atc gag ccc ctg ggc gtg gcc ccc       1488
Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro           
                485                 490                 495               

acc aag gcc aag cgc cgc gtg gtg cag cgc gag aag acc gga tcc tct       1536
Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Thr Gly Ser Ser           
            500                 505                 510                   

ggt ggc ggt ggc tcg ggc tcc gga gga ggt ggg tcg ggt ggc ggc gcg       1584
Gly Gly Gly Gly Ser Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ala           
        515                 520                 525                       

gcc gct cag gtc cag ctt gtg cag tct ggg gct gag gtg aag aag cct       1632
Ala Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro           
    530                 535                 540                           

ggg gcc tca gtg aag gtt tcc tgc cag gct tct gga tac agg ttc agc       1680
Gly Ala Ser Val Lys Val Ser Cys Gln Ala Ser Gly Tyr Arg Phe Ser           
545                 550                 555                 560           

aac ttc gtg atc cat tgg gtg cgc cag gcc ccc gga caa agg ttc gag       1728
Asn Phe Val Ile His Trp Val Arg Gln Ala Pro Gly Gln Arg Phe Glu           
                565                 570                 575               

tgg atg gga tgg atc aac cct tac aat ggt aac aag gag ttc tca gct       1776
Trp Met Gly Trp Ile Asn Pro Tyr Asn Gly Asn Lys Glu Phe Ser Ala           
            580                 585                 590                   

aag ttc cag gac aga gtc acc ttc acc gct gac aca tcc gcg aac aca       1824
Lys Phe Gln Asp Arg Val Thr Phe Thr Ala Asp Thr Ser Ala Asn Thr           
        595                 600                 605                       

gcc tac atg gag ctg agg agc ctg aga tct gct gac acg gct gtg tat       1872
Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Ala Asp Thr Ala Val Tyr           
    610                 615                 620                           

tac tgt gcg aga gtg gga ccc tac agc tgg gac gac agc ccc cag gac       1920
Tyr Cys Ala Arg Val Gly Pro Tyr Ser Trp Asp Asp Ser Pro Gln Asp           
625                 630                 635                 640           

aac tac tac atg gac gtc tgg ggc aaa ggg acc acg gtc atc gtc tcc       1968
Asn Tyr Tyr Met Asp Val Trp Gly Lys Gly Thr Thr Val Ile Val Ser           
                645                 650                 655               

tca gcg tcg acc ggc ggt ggc tct ggt ggc ggc ggt tcc ggt ggc ggt       2016
Ser Ala Ser Thr Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly           
            660                 665                 670                   

gga tcc gaa att gtg ttg acg cag tct cca ggc acc ctg tct ttg tct       2064
Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser           
        675                 680                 685                       

cca ggg gaa aga gcc acc ttc tcc tgc agg tcc agt cac agt att aga       2112
Pro Gly Glu Arg Ala Thr Phe Ser Cys Arg Ser Ser His Ser Ile Arg           
    690                 695                 700                           

agc aga agg gtg gcc tgg tac cag cac aaa cct ggc cag gct ccc agg       2160
Ser Arg Arg Val Ala Trp Tyr Gln His Lys Pro Gly Gln Ala Pro Arg           
705                 710                 715                 720           

ctc gtg atc cac ggt gtg tcc aac agg gcc agc ggc atc agc gac agg       2208
Leu Val Ile His Gly Val Ser Asn Arg Ala Ser Gly Ile Ser Asp Arg           
                725                 730                 735               

ttc agt ggc agt ggg tct ggg aca gac ttc act ctc acc atc acc aga       2256
Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Arg           
            740                 745                 750                   

gtg gag cct gaa gat ttt gca ctg tat tac tgt cag gtg tat ggt gcc       2304
Val Glu Pro Glu Asp Phe Ala Leu Tyr Tyr Cys Gln Val Tyr Gly Ala           
        755                 760                 765                       

tca agc tac act ttt ggc cag ggg acc aag ctg gag agg aaa tga           2349
Ser Ser Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Arg Lys                   
    770                 775                 780                           


<210>  8
<211>  782
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Met Arg Val Thr Glu Ile Arg Lys Ser Tyr Gln His Trp Trp Arg Trp 
1               5                   10                  15      


Gly Ile Met Leu Leu Gly Ile Leu Met Ile Cys Asn Ala Glu Glu Lys 
            20                  25                  30          


Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Thr 
        35                  40                  45              


Thr Thr Leu Phe Cys Ala Ser Asp Arg Lys Ala Tyr Asp Thr Glu Val 
    50                  55                  60                  


His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro 
65                  70                  75                  80  


Gln Glu Val Glu Leu Lys Asn Val Thr Glu Asn Phe Asn Met Trp Lys 
                85                  90                  95      


Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp 
            100                 105                 110         


Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu 
        115                 120                 125             


Asn Cys Thr Asp Leu Arg Asn Ala Thr Asn Gly Asn Asp Thr Asn Thr 
    130                 135                 140                 


Thr Ser Ser Ser Arg Gly Met Val Gly Gly Gly Glu Met Lys Asn Cys 
145                 150                 155                 160 


Ser Phe Asn Ile Thr Thr Asn Ile Arg Gly Lys Val Gln Lys Glu Tyr 
                165                 170                 175     


Ala Leu Phe Tyr Lys Leu Asp Ile Ala Pro Ile Asp Asn Asn Ser Asn 
            180                 185                 190         


Asn Arg Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln Ala 
        195                 200                 205             


Cys Pro Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro 
    210                 215                 220                 


Ala Gly Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Lys 
225                 230                 235                 240 


Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg 
                245                 250                 255     


Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu 
            260                 265                 270         


Glu Val Val Ile Arg Ser Ala Asn Phe Ala Asp Asn Ala Lys Val Ile 
        275                 280                 285             


Ile Val Gln Leu Asn Glu Ser Val Glu Ile Asn Cys Thr Arg Pro Asn 
    290                 295                 300                 


Asn Asn Thr Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr 
305                 310                 315                 320 


Thr Thr Gly Glu Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Leu 
                325                 330                 335     


Ser Arg Ala Lys Trp Asn Asp Thr Leu Asn Lys Ile Val Ile Lys Leu 
            340                 345                 350         


Arg Glu Gln Phe Gly Asn Lys Thr Ile Val Phe Lys His Ser Ser Gly 
        355                 360                 365             


Gly Asp Pro Glu Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe 
    370                 375                 380                 


Phe Tyr Cys Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp Asn Val Thr 
385                 390                 395                 400 


Glu Glu Ser Asn Asn Thr Val Glu Asn Asn Thr Ile Thr Leu Pro Cys 
                405                 410                 415     


Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met 
            420                 425                 430         


Tyr Ala Pro Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr 
        435                 440                 445             


Gly Leu Leu Leu Thr Arg Asp Gly Gly Pro Glu Asp Asn Lys Thr Glu 
    450                 455                 460                 


Val Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
465                 470                 475                 480 


Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro 
                485                 490                 495     


Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Thr Gly Ser Ser 
            500                 505                 510         


Gly Gly Gly Gly Ser Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ala 
        515                 520                 525             


Ala Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro 
    530                 535                 540                 


Gly Ala Ser Val Lys Val Ser Cys Gln Ala Ser Gly Tyr Arg Phe Ser 
545                 550                 555                 560 


Asn Phe Val Ile His Trp Val Arg Gln Ala Pro Gly Gln Arg Phe Glu 
                565                 570                 575     


Trp Met Gly Trp Ile Asn Pro Tyr Asn Gly Asn Lys Glu Phe Ser Ala 
            580                 585                 590         


Lys Phe Gln Asp Arg Val Thr Phe Thr Ala Asp Thr Ser Ala Asn Thr 
        595                 600                 605             


Ala Tyr Met Glu Leu Arg Ser Leu Arg Ser Ala Asp Thr Ala Val Tyr 
    610                 615                 620                 


Tyr Cys Ala Arg Val Gly Pro Tyr Ser Trp Asp Asp Ser Pro Gln Asp 
625                 630                 635                 640 


Asn Tyr Tyr Met Asp Val Trp Gly Lys Gly Thr Thr Val Ile Val Ser 
                645                 650                 655     


Ser Ala Ser Thr Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
            660                 665                 670         


Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser 
        675                 680                 685             


Pro Gly Glu Arg Ala Thr Phe Ser Cys Arg Ser Ser His Ser Ile Arg 
    690                 695                 700                 


Ser Arg Arg Val Ala Trp Tyr Gln His Lys Pro Gly Gln Ala Pro Arg 
705                 710                 715                 720 


Leu Val Ile His Gly Val Ser Asn Arg Ala Ser Gly Ile Ser Asp Arg 
                725                 730                 735     


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Arg 
            740                 745                 750         


Val Glu Pro Glu Asp Phe Ala Leu Tyr Tyr Cys Gln Val Tyr Gly Ala 
        755                 760                 765             


Ser Ser Tyr Thr Phe Gly Gln Gly Thr Lys Leu Glu Arg Lys 
    770                 775                 780         


<210>  9
<211>  2316
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized gp120-ScFV construct comprising 
       gp120-VRC01-ScFV


<220>
<221>  CDS
<222>  (1)..(2316)

<400>  9
atg aga gtg acc gag atc aga aag tcc tac cag cat tgg tgg aga tgg         48
Met Arg Val Thr Glu Ile Arg Lys Ser Tyr Gln His Trp Trp Arg Trp           
1               5                   10                  15                

gga atc atg ctg ctc gga atc ctg atg atc tgc aac gcc gag gag aag         96
Gly Ile Met Leu Leu Gly Ile Leu Met Ile Cys Asn Ala Glu Glu Lys           
            20                  25                  30                    

ctg tgg gtg acc gtg tac tac ggc gtg ccc gtg tgg aag gag gcc acc        144
Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Thr           
        35                  40                  45                        

acc acc ctg ttc tgc gcc agc gac cgc aag gcc tac gac acc gag gtg        192
Thr Thr Leu Phe Cys Ala Ser Asp Arg Lys Ala Tyr Asp Thr Glu Val           
    50                  55                  60                            

cac aac gtg tgg gcc acc cac gcc tgc gtg ccc acc gac ccc aac ccc        240
His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro           
65                  70                  75                  80            

cag gag gtg gag ctg aag aac gtg acc gag aac ttc aac atg tgg aag        288
Gln Glu Val Glu Leu Lys Asn Val Thr Glu Asn Phe Asn Met Trp Lys           
                85                  90                  95                

aac aac atg gtg gag cag atg cac gag gac atc atc agc ctg tgg gac        336
Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp           
            100                 105                 110                   

cag agc ctg aag ccc tgc gtg aag ctg acc ccc ctg tgc gtg acc ctg        384
Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu           
        115                 120                 125                       

aac tgc acc gac ctg cgc aac gcc acc aac ggc aac gac acc aac acc        432
Asn Cys Thr Asp Leu Arg Asn Ala Thr Asn Gly Asn Asp Thr Asn Thr           
    130                 135                 140                           

act agt agc agc cgc ggc atg gtg ggc ggc ggc gag atg aag aac tgc        480
Thr Ser Ser Ser Arg Gly Met Val Gly Gly Gly Glu Met Lys Asn Cys           
145                 150                 155                 160           

agc ttc aac atc acc acc aac atc cgc ggc aag gtg cag aag gag tac        528
Ser Phe Asn Ile Thr Thr Asn Ile Arg Gly Lys Val Gln Lys Glu Tyr           
                165                 170                 175               

gcc ctg ttc tac aag ctg gac atc gcc ccc atc gac aac aac agc aac        576
Ala Leu Phe Tyr Lys Leu Asp Ile Ala Pro Ile Asp Asn Asn Ser Asn           
            180                 185                 190                   

aac cgc tac cgc ctg atc agc tgc aac acc agc gtg atc acc cag gcc        624
Asn Arg Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln Ala           
        195                 200                 205                       

tgc ccc aag gtg agc ttc gag ccc atc ccc atc cac tac tgc gcc ccc        672
Cys Pro Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro           
    210                 215                 220                           

gcc ggc ttc gcc atc ctg aag tgc aag gac aag aag ttc aac ggc aag        720
Ala Gly Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Lys           
225                 230                 235                 240           

ggc ccc tgc acc aac gtg agc acc gtg cag tgc acc cac ggc atc cgc        768
Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg           
                245                 250                 255               

ccc gtg gtg agc acc cag ctg ctg ctg aac ggc agc ctg gcc gag gag        816
Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu           
            260                 265                 270                   

gag gtg gtg atc cgc agc gcc aac ttc gcc gac aac gcc aag gtg atc        864
Glu Val Val Ile Arg Ser Ala Asn Phe Ala Asp Asn Ala Lys Val Ile           
        275                 280                 285                       

atc gtg cag ctg aac gag agc gtg gag atc aac tgc acc cgc ccc aac        912
Ile Val Gln Leu Asn Glu Ser Val Glu Ile Asn Cys Thr Arg Pro Asn           
    290                 295                 300                           

aac aac acc cgc aag tcc atc cac atc ggc ccc ggc cgc gcc ttc tac        960
Asn Asn Thr Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr           
305                 310                 315                 320           

acc acc ggc gag atc atc ggc gac atc cgc cag gcc cac tgc aac ctg       1008
Thr Thr Gly Glu Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Leu           
                325                 330                 335               

agc cgc gcc aag tgg aac gac acc ctg aac aag atc gtg atc aag ctg       1056
Ser Arg Ala Lys Trp Asn Asp Thr Leu Asn Lys Ile Val Ile Lys Leu           
            340                 345                 350                   

cgc gag cag ttc ggc aac aag acc atc gtg ttc aag cac agc agc ggc       1104
Arg Glu Gln Phe Gly Asn Lys Thr Ile Val Phe Lys His Ser Ser Gly           
        355                 360                 365                       

ggc gac ccc gag atc gtg acc cac agc ttc aat tgc ggc ggc gag ttc       1152
Gly Asp Pro Glu Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe           
    370                 375                 380                           

ttc tac tgc aac agc acc cag ctg ttc aac agc acc tgg aac gtg acc       1200
Phe Tyr Cys Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp Asn Val Thr           
385                 390                 395                 400           

gag gag agc aac aac acc gtg gag aac aac acc atc acc ctg ccc tgc       1248
Glu Glu Ser Asn Asn Thr Val Glu Asn Asn Thr Ile Thr Leu Pro Cys           
                405                 410                 415               

cgc atc aag cag atc atc aac atg tgg cag gag gtg ggc cgc gcc atg       1296
Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met           
            420                 425                 430                   

tac gcc ccc ccc atc cgc ggc cag atc cgc tgc agt tcg aac atc acc       1344
Tyr Ala Pro Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr           
        435                 440                 445                       

ggc ctg ctg ctg acc cgc gac ggc ggc ccc gag gac aac aag acc gag       1392
Gly Leu Leu Leu Thr Arg Asp Gly Gly Pro Glu Asp Asn Lys Thr Glu           
    450                 455                 460                           

gtg ttc cgc ccc ggc ggc ggc gac atg cgc gac aac tgg cgc agc gag       1440
Val Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu           
465                 470                 475                 480           

ctg tac aag tac aag gtg gtg aag atc gag ccc ctg ggc gtg gcc ccc       1488
Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro           
                485                 490                 495               

acc aag gcc aag cgc cgc gtg gtg cag cgc gag aag acc gga tcc tct       1536
Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Thr Gly Ser Ser           
            500                 505                 510                   

ggt ggc ggt ggc tcg ggc tcc gga gga ggt ggg tcg ggt ggc ggc gcg       1584
Gly Gly Gly Gly Ser Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ala           
        515                 520                 525                       

gcc gct cag gtg cag ctg gtg cag tct ggg ggt cag atg aag aag cct       1632
Ala Ala Gln Val Gln Leu Val Gln Ser Gly Gly Gln Met Lys Lys Pro           
    530                 535                 540                           

ggc gag tcg atg aga att tct tgt cgg gct tct gga tat gaa ttt att       1680
Gly Glu Ser Met Arg Ile Ser Cys Arg Ala Ser Gly Tyr Glu Phe Ile           
545                 550                 555                 560           

gat tgt acg cta aat tgg att cgt ctg gcc ccc gga aaa agg cct gag       1728
Asp Cys Thr Leu Asn Trp Ile Arg Leu Ala Pro Gly Lys Arg Pro Glu           
                565                 570                 575               

tgg atg gga tgg ctg aag cct cgg ggg ggg gcc gtc aac tac gca cgt       1776
Trp Met Gly Trp Leu Lys Pro Arg Gly Gly Ala Val Asn Tyr Ala Arg           
            580                 585                 590                   

cca ctt cag ggc aga gtg acc atg act cga gac gtt tat tcc gac aca       1824
Pro Leu Gln Gly Arg Val Thr Met Thr Arg Asp Val Tyr Ser Asp Thr           
        595                 600                 605                       

gcc ttt ttg gag ctg cgc tcg ttg aca gta gac gac acg gcc gtc tac       1872
Ala Phe Leu Glu Leu Arg Ser Leu Thr Val Asp Asp Thr Ala Val Tyr           
    610                 615                 620                           

ttt tgt act agg gga aaa aac tgt gat tac aat tgg gac ttc gaa cac       1920
Phe Cys Thr Arg Gly Lys Asn Cys Asp Tyr Asn Trp Asp Phe Glu His           
625                 630                 635                 640           

tgg ggc cgg ggc acc ccg gtc atc gtc tca tca gcg tcg acc ggc ggt       1968
Trp Gly Arg Gly Thr Pro Val Ile Val Ser Ser Ala Ser Thr Gly Gly           
                645                 650                 655               

ggc tct ggt ggc ggc ggt tcc ggt ggc ggt gga tcc gaa att gtg ttg       2016
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu           
            660                 665                 670                   

aca cag tct cca ggc acc ctg tct ttg tct cca ggg gaa aca gcc atc       2064
Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Thr Ala Ile           
        675                 680                 685                       

atc tct tgt cgg acc agt cag tat ggt tcc tta gcc tgg tat caa cag       2112
Ile Ser Cys Arg Thr Ser Gln Tyr Gly Ser Leu Ala Trp Tyr Gln Gln           
    690                 695                 700                           

agg ccc ggc cag gcc ccc agg ctc gtc atc tat tcg ggc tct act cgg       2160
Arg Pro Gly Gln Ala Pro Arg Leu Val Ile Tyr Ser Gly Ser Thr Arg           
705                 710                 715                 720           

gcc gct ggc atc cca gac agg ttc agc ggc agt cgg tgg ggg cca gac       2208
Ala Ala Gly Ile Pro Asp Arg Phe Ser Gly Ser Arg Trp Gly Pro Asp           
                725                 730                 735               

tac aat ctc acc atc agc aac ctg gag tcg gga gat ttt ggt gtt tat       2256
Tyr Asn Leu Thr Ile Ser Asn Leu Glu Ser Gly Asp Phe Gly Val Tyr           
            740                 745                 750                   

tat tgc cag cag tat gaa ttt ttt ggc cag ggg acc aag gtc cag gtc       2304
Tyr Cys Gln Gln Tyr Glu Phe Phe Gly Gln Gly Thr Lys Val Gln Val           
        755                 760                 765                       

gac att aag tga                                                       2316
Asp Ile Lys                                                               
    770                                                                   


<210>  10
<211>  771
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  10

Met Arg Val Thr Glu Ile Arg Lys Ser Tyr Gln His Trp Trp Arg Trp 
1               5                   10                  15      


Gly Ile Met Leu Leu Gly Ile Leu Met Ile Cys Asn Ala Glu Glu Lys 
            20                  25                  30          


Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Thr 
        35                  40                  45              


Thr Thr Leu Phe Cys Ala Ser Asp Arg Lys Ala Tyr Asp Thr Glu Val 
    50                  55                  60                  


His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro 
65                  70                  75                  80  


Gln Glu Val Glu Leu Lys Asn Val Thr Glu Asn Phe Asn Met Trp Lys 
                85                  90                  95      


Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp 
            100                 105                 110         


Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu 
        115                 120                 125             


Asn Cys Thr Asp Leu Arg Asn Ala Thr Asn Gly Asn Asp Thr Asn Thr 
    130                 135                 140                 


Thr Ser Ser Ser Arg Gly Met Val Gly Gly Gly Glu Met Lys Asn Cys 
145                 150                 155                 160 


Ser Phe Asn Ile Thr Thr Asn Ile Arg Gly Lys Val Gln Lys Glu Tyr 
                165                 170                 175     


Ala Leu Phe Tyr Lys Leu Asp Ile Ala Pro Ile Asp Asn Asn Ser Asn 
            180                 185                 190         


Asn Arg Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln Ala 
        195                 200                 205             


Cys Pro Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro 
    210                 215                 220                 


Ala Gly Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Lys 
225                 230                 235                 240 


Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg 
                245                 250                 255     


Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu 
            260                 265                 270         


Glu Val Val Ile Arg Ser Ala Asn Phe Ala Asp Asn Ala Lys Val Ile 
        275                 280                 285             


Ile Val Gln Leu Asn Glu Ser Val Glu Ile Asn Cys Thr Arg Pro Asn 
    290                 295                 300                 


Asn Asn Thr Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr 
305                 310                 315                 320 


Thr Thr Gly Glu Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Leu 
                325                 330                 335     


Ser Arg Ala Lys Trp Asn Asp Thr Leu Asn Lys Ile Val Ile Lys Leu 
            340                 345                 350         


Arg Glu Gln Phe Gly Asn Lys Thr Ile Val Phe Lys His Ser Ser Gly 
        355                 360                 365             


Gly Asp Pro Glu Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe 
    370                 375                 380                 


Phe Tyr Cys Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp Asn Val Thr 
385                 390                 395                 400 


Glu Glu Ser Asn Asn Thr Val Glu Asn Asn Thr Ile Thr Leu Pro Cys 
                405                 410                 415     


Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met 
            420                 425                 430         


Tyr Ala Pro Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr 
        435                 440                 445             


Gly Leu Leu Leu Thr Arg Asp Gly Gly Pro Glu Asp Asn Lys Thr Glu 
    450                 455                 460                 


Val Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
465                 470                 475                 480 


Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro 
                485                 490                 495     


Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Thr Gly Ser Ser 
            500                 505                 510         


Gly Gly Gly Gly Ser Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ala 
        515                 520                 525             


Ala Ala Gln Val Gln Leu Val Gln Ser Gly Gly Gln Met Lys Lys Pro 
    530                 535                 540                 


Gly Glu Ser Met Arg Ile Ser Cys Arg Ala Ser Gly Tyr Glu Phe Ile 
545                 550                 555                 560 


Asp Cys Thr Leu Asn Trp Ile Arg Leu Ala Pro Gly Lys Arg Pro Glu 
                565                 570                 575     


Trp Met Gly Trp Leu Lys Pro Arg Gly Gly Ala Val Asn Tyr Ala Arg 
            580                 585                 590         


Pro Leu Gln Gly Arg Val Thr Met Thr Arg Asp Val Tyr Ser Asp Thr 
        595                 600                 605             


Ala Phe Leu Glu Leu Arg Ser Leu Thr Val Asp Asp Thr Ala Val Tyr 
    610                 615                 620                 


Phe Cys Thr Arg Gly Lys Asn Cys Asp Tyr Asn Trp Asp Phe Glu His 
625                 630                 635                 640 


Trp Gly Arg Gly Thr Pro Val Ile Val Ser Ser Ala Ser Thr Gly Gly 
                645                 650                 655     


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu 
            660                 665                 670         


Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly Glu Thr Ala Ile 
        675                 680                 685             


Ile Ser Cys Arg Thr Ser Gln Tyr Gly Ser Leu Ala Trp Tyr Gln Gln 
    690                 695                 700                 


Arg Pro Gly Gln Ala Pro Arg Leu Val Ile Tyr Ser Gly Ser Thr Arg 
705                 710                 715                 720 


Ala Ala Gly Ile Pro Asp Arg Phe Ser Gly Ser Arg Trp Gly Pro Asp 
                725                 730                 735     


Tyr Asn Leu Thr Ile Ser Asn Leu Glu Ser Gly Asp Phe Gly Val Tyr 
            740                 745                 750         


Tyr Cys Gln Gln Tyr Glu Phe Phe Gly Gln Gly Thr Lys Val Gln Val 
        755                 760                 765             


Asp Ile Lys 
    770     


<210>  11
<211>  2343
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized gp120-ScFV construct comprising 
       gp120-JR4-ScFV


<220>
<221>  CDS
<222>  (1)..(2343)

<400>  11
atg aga gtg acc gag atc aga aag tcc tac cag cat tgg tgg aga tgg         48
Met Arg Val Thr Glu Ile Arg Lys Ser Tyr Gln His Trp Trp Arg Trp           
1               5                   10                  15                

gga atc atg ctg ctc gga atc ctg atg atc tgc aac gcc gag gag aag         96
Gly Ile Met Leu Leu Gly Ile Leu Met Ile Cys Asn Ala Glu Glu Lys           
            20                  25                  30                    

ctg tgg gtg acc gtg tac tac ggc gtg ccc gtg tgg aag gag gcc acc        144
Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Thr           
        35                  40                  45                        

acc acc ctg ttc tgc gcc agc gac cgc aag gcc tac gac acc gag gtg        192
Thr Thr Leu Phe Cys Ala Ser Asp Arg Lys Ala Tyr Asp Thr Glu Val           
    50                  55                  60                            

cac aac gtg tgg gcc acc cac gcc tgc gtg ccc acc gac ccc aac ccc        240
His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro           
65                  70                  75                  80            

cag gag gtg gag ctg aag aac gtg acc gag aac ttc aac atg tgg aag        288
Gln Glu Val Glu Leu Lys Asn Val Thr Glu Asn Phe Asn Met Trp Lys           
                85                  90                  95                

aac aac atg gtg gag cag atg cac gag gac atc atc agc ctg tgg gac        336
Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp           
            100                 105                 110                   

cag agc ctg aag ccc tgc gtg aag ctg acc ccc ctg tgc gtg acc ctg        384
Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu           
        115                 120                 125                       

aac tgc acc gac ctg cgc aac gcc acc aac ggc aac gac acc aac acc        432
Asn Cys Thr Asp Leu Arg Asn Ala Thr Asn Gly Asn Asp Thr Asn Thr           
    130                 135                 140                           

act agt agc agc cgc ggc atg gtg ggc ggc ggc gag atg aag aac tgc        480
Thr Ser Ser Ser Arg Gly Met Val Gly Gly Gly Glu Met Lys Asn Cys           
145                 150                 155                 160           

agc ttc aac atc acc acc aac atc cgc ggc aag gtg cag aag gag tac        528
Ser Phe Asn Ile Thr Thr Asn Ile Arg Gly Lys Val Gln Lys Glu Tyr           
                165                 170                 175               

gcc ctg ttc tac aag ctg gac atc gcc ccc atc gac aac aac agc aac        576
Ala Leu Phe Tyr Lys Leu Asp Ile Ala Pro Ile Asp Asn Asn Ser Asn           
            180                 185                 190                   

aac cgc tac cgc ctg atc agc tgc aac acc agc gtg atc acc cag gcc        624
Asn Arg Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln Ala           
        195                 200                 205                       

tgc ccc aag gtg agc ttc gag ccc atc ccc atc cac tac tgc gcc ccc        672
Cys Pro Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro           
    210                 215                 220                           

gcc ggc ttc gcc atc ctg aag tgc aag gac aag aag ttc aac ggc aag        720
Ala Gly Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Lys           
225                 230                 235                 240           

ggc ccc tgc acc aac gtg agc acc gtg cag tgc acc cac ggc atc cgc        768
Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg           
                245                 250                 255               

ccc gtg gtg agc acc cag ctg ctg ctg aac ggc agc ctg gcc gag gag        816
Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu           
            260                 265                 270                   

gag gtg gtg atc cgc agc gcc aac ttc gcc gac aac gcc aag gtg atc        864
Glu Val Val Ile Arg Ser Ala Asn Phe Ala Asp Asn Ala Lys Val Ile           
        275                 280                 285                       

atc gtg cag ctg aac gag agc gtg gag atc aac tgc acc cgc ccc aac        912
Ile Val Gln Leu Asn Glu Ser Val Glu Ile Asn Cys Thr Arg Pro Asn           
    290                 295                 300                           

aac aac acc cgc aag tcc atc cac atc ggc ccc ggc cgc gcc ttc tac        960
Asn Asn Thr Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr           
305                 310                 315                 320           

acc acc ggc gag atc atc ggc gac atc cgc cag gcc cac tgc aac ctg       1008
Thr Thr Gly Glu Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Leu           
                325                 330                 335               

agc cgc gcc aag tgg aac gac acc ctg aac aag atc gtg atc aag ctg       1056
Ser Arg Ala Lys Trp Asn Asp Thr Leu Asn Lys Ile Val Ile Lys Leu           
            340                 345                 350                   

cgc gag cag ttc ggc aac aag acc atc gtg ttc aag cac agc agc ggc       1104
Arg Glu Gln Phe Gly Asn Lys Thr Ile Val Phe Lys His Ser Ser Gly           
        355                 360                 365                       

ggc gac ccc gag atc gtg acc cac agc ttc aat tgc ggc ggc gag ttc       1152
Gly Asp Pro Glu Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe           
    370                 375                 380                           

ttc tac tgc aac agc acc cag ctg ttc aac agc acc tgg aac gtg acc       1200
Phe Tyr Cys Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp Asn Val Thr           
385                 390                 395                 400           

gag gag agc aac aac acc gtg gag aac aac acc atc acc ctg ccc tgc       1248
Glu Glu Ser Asn Asn Thr Val Glu Asn Asn Thr Ile Thr Leu Pro Cys           
                405                 410                 415               

cgc atc aag cag atc atc aac atg tgg cag gag gtg ggc cgc gcc atg       1296
Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met           
            420                 425                 430                   

tac gcc ccc ccc atc cgc ggc cag atc cgc tgc agt tcg aac atc acc       1344
Tyr Ala Pro Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr           
        435                 440                 445                       

ggc ctg ctg ctg acc cgc gac ggc ggc ccc gag gac aac aag acc gag       1392
Gly Leu Leu Leu Thr Arg Asp Gly Gly Pro Glu Asp Asn Lys Thr Glu           
    450                 455                 460                           

gtg ttc cgc ccc ggc ggc ggc gac atg cgc gac aac tgg cgc agc gag       1440
Val Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu           
465                 470                 475                 480           

ctg tac aag tac aag gtg gtg aag atc gag ccc ctg ggc gtg gcc ccc       1488
Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro           
                485                 490                 495               

acc aag gcc aag cgc cgc gtg gtg cag cgc gag aag acc gga tcc tct       1536
Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Thr Gly Ser Ser           
            500                 505                 510                   

ggt ggc ggt ggc tcg ggc tcc gga gga ggt ggg tcg ggt ggc ggc gcg       1584
Gly Gly Gly Gly Ser Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ala           
        515                 520                 525                       

gcc gct gag gtg cag ctg gtg gag tcg ggc cca gga ctg gtg aag cct       1632
Ala Ala Glu Val Gln Leu Val Glu Ser Gly Pro Gly Leu Val Lys Pro           
    530                 535                 540                           

ttg gag act ctg tcc ctc acc tgc gct gtc cct ggt ggc tct atc aga       1680
Leu Glu Thr Leu Ser Leu Thr Cys Ala Val Pro Gly Gly Ser Ile Arg           
545                 550                 555                 560           

aga aac tac tgg agc tgg atc cgc cag ccc cca gga aag gga ctg gag       1728
Arg Asn Tyr Trp Ser Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu           
                565                 570                 575               

tgg att ggg cat tcc tat ggt agc gga ggt tct acc aac tac aac ccc       1776
Trp Ile Gly His Ser Tyr Gly Ser Gly Gly Ser Thr Asn Tyr Asn Pro           
            580                 585                 590                   

tcc ctc gag agt cga gtc acc ctg tca gta gat acg tcc aag aat ctt       1824
Ser Leu Glu Ser Arg Val Thr Leu Ser Val Asp Thr Ser Lys Asn Leu           
        595                 600                 605                       

ttc tcc ctg aag ctg acc tct gtg acc gcc gcg gac acg gcc gtt tat       1872
Phe Ser Leu Lys Leu Thr Ser Val Thr Ala Ala Asp Thr Ala Val Tyr           
    610                 615                 620                           

tac tgt gcg agg acc gtc tgg tat tac act agt ggt acc cat tac ttt       1920
Tyr Cys Ala Arg Thr Val Trp Tyr Tyr Thr Ser Gly Thr His Tyr Phe           
625                 630                 635                 640           

gac cac tgg ggc cag gga gtc ctg gtc act gtc tcc tca gcg tcg acc       1968
Asp His Trp Gly Gln Gly Val Leu Val Thr Val Ser Ser Ala Ser Thr           
                645                 650                 655               

ggc ggt ggc tct ggt ggc ggc ggt tcc ggt ggc ggt gga tcc cag tct       2016
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ser           
            660                 665                 670                   

gtg ttg acg cag ccg ccc tca gtg tct gcg gcc cca ggg cag aag gtc       2064
Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln Lys Val           
        675                 680                 685                       

acc atc tcc tgc tct gga agc agc tcc aac atc ggg aga agt tat gta       2112
Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Arg Ser Tyr Val           
    690                 695                 700                           

tct tgg tac cag cag gtc cca gga gcg gcc ccc aag ctc ctc atc tat       2160
Ser Trp Tyr Gln Gln Val Pro Gly Ala Ala Pro Lys Leu Leu Ile Tyr           
705                 710                 715                 720           

gac act aat aag cga ccc tca ggg gtt tct gac cga ttc tct ggc tcc       2208
Asp Thr Asn Lys Arg Pro Ser Gly Val Ser Asp Arg Phe Ser Gly Ser           
                725                 730                 735               

aag tct ggt tcc tcg gcc tcc ctg gcc atc act gga ctc caa act gga       2256
Lys Ser Gly Ser Ser Ala Ser Leu Ala Ile Thr Gly Leu Gln Thr Gly           
            740                 745                 750                   

gat gag gct gat tat tac tgc gga gca tgg gat ggc agc ctg aat gtt       2304
Asp Glu Ala Asp Tyr Tyr Cys Gly Ala Trp Asp Gly Ser Leu Asn Val           
        755                 760                 765                       

cat ata ttc gga agt ggc acc aag ttg acc gtc ctc tga                   2343
His Ile Phe Gly Ser Gly Thr Lys Leu Thr Val Leu                           
    770                 775                 780                           


<210>  12
<211>  780
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12

Met Arg Val Thr Glu Ile Arg Lys Ser Tyr Gln His Trp Trp Arg Trp 
1               5                   10                  15      


Gly Ile Met Leu Leu Gly Ile Leu Met Ile Cys Asn Ala Glu Glu Lys 
            20                  25                  30          


Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Thr 
        35                  40                  45              


Thr Thr Leu Phe Cys Ala Ser Asp Arg Lys Ala Tyr Asp Thr Glu Val 
    50                  55                  60                  


His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro 
65                  70                  75                  80  


Gln Glu Val Glu Leu Lys Asn Val Thr Glu Asn Phe Asn Met Trp Lys 
                85                  90                  95      


Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp 
            100                 105                 110         


Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu 
        115                 120                 125             


Asn Cys Thr Asp Leu Arg Asn Ala Thr Asn Gly Asn Asp Thr Asn Thr 
    130                 135                 140                 


Thr Ser Ser Ser Arg Gly Met Val Gly Gly Gly Glu Met Lys Asn Cys 
145                 150                 155                 160 


Ser Phe Asn Ile Thr Thr Asn Ile Arg Gly Lys Val Gln Lys Glu Tyr 
                165                 170                 175     


Ala Leu Phe Tyr Lys Leu Asp Ile Ala Pro Ile Asp Asn Asn Ser Asn 
            180                 185                 190         


Asn Arg Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln Ala 
        195                 200                 205             


Cys Pro Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro 
    210                 215                 220                 


Ala Gly Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Lys 
225                 230                 235                 240 


Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg 
                245                 250                 255     


Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu 
            260                 265                 270         


Glu Val Val Ile Arg Ser Ala Asn Phe Ala Asp Asn Ala Lys Val Ile 
        275                 280                 285             


Ile Val Gln Leu Asn Glu Ser Val Glu Ile Asn Cys Thr Arg Pro Asn 
    290                 295                 300                 


Asn Asn Thr Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr 
305                 310                 315                 320 


Thr Thr Gly Glu Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Leu 
                325                 330                 335     


Ser Arg Ala Lys Trp Asn Asp Thr Leu Asn Lys Ile Val Ile Lys Leu 
            340                 345                 350         


Arg Glu Gln Phe Gly Asn Lys Thr Ile Val Phe Lys His Ser Ser Gly 
        355                 360                 365             


Gly Asp Pro Glu Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe 
    370                 375                 380                 


Phe Tyr Cys Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp Asn Val Thr 
385                 390                 395                 400 


Glu Glu Ser Asn Asn Thr Val Glu Asn Asn Thr Ile Thr Leu Pro Cys 
                405                 410                 415     


Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met 
            420                 425                 430         


Tyr Ala Pro Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr 
        435                 440                 445             


Gly Leu Leu Leu Thr Arg Asp Gly Gly Pro Glu Asp Asn Lys Thr Glu 
    450                 455                 460                 


Val Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
465                 470                 475                 480 


Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro 
                485                 490                 495     


Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Thr Gly Ser Ser 
            500                 505                 510         


Gly Gly Gly Gly Ser Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ala 
        515                 520                 525             


Ala Ala Glu Val Gln Leu Val Glu Ser Gly Pro Gly Leu Val Lys Pro 
    530                 535                 540                 


Leu Glu Thr Leu Ser Leu Thr Cys Ala Val Pro Gly Gly Ser Ile Arg 
545                 550                 555                 560 


Arg Asn Tyr Trp Ser Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu 
                565                 570                 575     


Trp Ile Gly His Ser Tyr Gly Ser Gly Gly Ser Thr Asn Tyr Asn Pro 
            580                 585                 590         


Ser Leu Glu Ser Arg Val Thr Leu Ser Val Asp Thr Ser Lys Asn Leu 
        595                 600                 605             


Phe Ser Leu Lys Leu Thr Ser Val Thr Ala Ala Asp Thr Ala Val Tyr 
    610                 615                 620                 


Tyr Cys Ala Arg Thr Val Trp Tyr Tyr Thr Ser Gly Thr His Tyr Phe 
625                 630                 635                 640 


Asp His Trp Gly Gln Gly Val Leu Val Thr Val Ser Ser Ala Ser Thr 
                645                 650                 655     


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ser 
            660                 665                 670         


Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln Lys Val 
        675                 680                 685             


Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Arg Ser Tyr Val 
    690                 695                 700                 


Ser Trp Tyr Gln Gln Val Pro Gly Ala Ala Pro Lys Leu Leu Ile Tyr 
705                 710                 715                 720 


Asp Thr Asn Lys Arg Pro Ser Gly Val Ser Asp Arg Phe Ser Gly Ser 
                725                 730                 735     


Lys Ser Gly Ser Ser Ala Ser Leu Ala Ile Thr Gly Leu Gln Thr Gly 
            740                 745                 750         


Asp Glu Ala Asp Tyr Tyr Cys Gly Ala Trp Asp Gly Ser Leu Asn Val 
        755                 760                 765             


His Ile Phe Gly Ser Gly Thr Lys Leu Thr Val Leu 
    770                 775                 780 


<210>  13
<211>  2334
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chemically-synthesized gp120-ScFV construct comprising 
       gp120-JR48.1-ScFV


<220>
<221>  CDS
<222>  (1)..(2334)

<400>  13
atg aga gtg acc gag atc aga aag tcc tac cag cat tgg tgg aga tgg         48
Met Arg Val Thr Glu Ile Arg Lys Ser Tyr Gln His Trp Trp Arg Trp           
1               5                   10                  15                

gga atc atg ctg ctc gga atc ctg atg atc tgc aac gcc gag gag aag         96
Gly Ile Met Leu Leu Gly Ile Leu Met Ile Cys Asn Ala Glu Glu Lys           
            20                  25                  30                    

ctg tgg gtg acc gtg tac tac ggc gtg ccc gtg tgg aag gag gcc acc        144
Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Thr           
        35                  40                  45                        

acc acc ctg ttc tgc gcc agc gac cgc aag gcc tac gac acc gag gtg        192
Thr Thr Leu Phe Cys Ala Ser Asp Arg Lys Ala Tyr Asp Thr Glu Val           
    50                  55                  60                            

cac aac gtg tgg gcc acc cac gcc tgc gtg ccc acc gac ccc aac ccc        240
His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro           
65                  70                  75                  80            

cag gag gtg gag ctg aag aac gtg acc gag aac ttc aac atg tgg aag        288
Gln Glu Val Glu Leu Lys Asn Val Thr Glu Asn Phe Asn Met Trp Lys           
                85                  90                  95                

aac aac atg gtg gag cag atg cac gag gac atc atc agc ctg tgg gac        336
Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp           
            100                 105                 110                   

cag agc ctg aag ccc tgc gtg aag ctg acc ccc ctg tgc gtg acc ctg        384
Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu           
        115                 120                 125                       

aac tgc acc gac ctg cgc aac gcc acc aac ggc aac gac acc aac acc        432
Asn Cys Thr Asp Leu Arg Asn Ala Thr Asn Gly Asn Asp Thr Asn Thr           
    130                 135                 140                           

act agt agc agc cgc ggc atg gtg ggc ggc ggc gag atg aag aac tgc        480
Thr Ser Ser Ser Arg Gly Met Val Gly Gly Gly Glu Met Lys Asn Cys           
145                 150                 155                 160           

agc ttc aac atc acc acc aac atc cgc ggc aag gtg cag aag gag tac        528
Ser Phe Asn Ile Thr Thr Asn Ile Arg Gly Lys Val Gln Lys Glu Tyr           
                165                 170                 175               

gcc ctg ttc tac aag ctg gac atc gcc ccc atc gac aac aac agc aac        576
Ala Leu Phe Tyr Lys Leu Asp Ile Ala Pro Ile Asp Asn Asn Ser Asn           
            180                 185                 190                   

aac cgc tac cgc ctg atc agc tgc aac acc agc gtg atc acc cag gcc        624
Asn Arg Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln Ala           
        195                 200                 205                       

tgc ccc aag gtg agc ttc gag ccc atc ccc atc cac tac tgc gcc ccc        672
Cys Pro Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro           
    210                 215                 220                           

gcc ggc ttc gcc atc ctg aag tgc aag gac aag aag ttc aac ggc aag        720
Ala Gly Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Lys           
225                 230                 235                 240           

ggc ccc tgc acc aac gtg agc acc gtg cag tgc acc cac ggc atc cgc        768
Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg           
                245                 250                 255               

ccc gtg gtg agc acc cag ctg ctg ctg aac ggc agc ctg gcc gag gag        816
Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu           
            260                 265                 270                   

gag gtg gtg atc cgc agc gcc aac ttc gcc gac aac gcc aag gtg atc        864
Glu Val Val Ile Arg Ser Ala Asn Phe Ala Asp Asn Ala Lys Val Ile           
        275                 280                 285                       

atc gtg cag ctg aac gag agc gtg gag atc aac tgc acc cgc ccc aac        912
Ile Val Gln Leu Asn Glu Ser Val Glu Ile Asn Cys Thr Arg Pro Asn           
    290                 295                 300                           

aac aac acc cgc aag tcc atc cac atc ggc ccc ggc cgc gcc ttc tac        960
Asn Asn Thr Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr           
305                 310                 315                 320           

acc acc ggc gag atc atc ggc gac atc cgc cag gcc cac tgc aac ctg       1008
Thr Thr Gly Glu Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Leu           
                325                 330                 335               

agc cgc gcc aag tgg aac gac acc ctg aac aag atc gtg atc aag ctg       1056
Ser Arg Ala Lys Trp Asn Asp Thr Leu Asn Lys Ile Val Ile Lys Leu           
            340                 345                 350                   

cgc gag cag ttc ggc aac aag acc atc gtg ttc aag cac agc agc ggc       1104
Arg Glu Gln Phe Gly Asn Lys Thr Ile Val Phe Lys His Ser Ser Gly           
        355                 360                 365                       

ggc gac ccc gag atc gtg acc cac agc ttc aat tgc ggc ggc gag ttc       1152
Gly Asp Pro Glu Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe           
    370                 375                 380                           

ttc tac tgc aac agc acc cag ctg ttc aac agc acc tgg aac gtg acc       1200
Phe Tyr Cys Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp Asn Val Thr           
385                 390                 395                 400           

gag gag agc aac aac acc gtg gag aac aac acc atc acc ctg ccc tgc       1248
Glu Glu Ser Asn Asn Thr Val Glu Asn Asn Thr Ile Thr Leu Pro Cys           
                405                 410                 415               

cgc atc aag cag atc atc aac atg tgg cag gag gtg ggc cgc gcc atg       1296
Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met           
            420                 425                 430                   

tac gcc ccc ccc atc cgc ggc cag atc cgc tgc agt tcg aac atc acc       1344
Tyr Ala Pro Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr           
        435                 440                 445                       

ggc ctg ctg ctg acc cgc gac ggc ggc ccc gag gac aac aag acc gag       1392
Gly Leu Leu Leu Thr Arg Asp Gly Gly Pro Glu Asp Asn Lys Thr Glu           
    450                 455                 460                           

gtg ttc cgc ccc ggc ggc ggc gac atg cgc gac aac tgg cgc agc gag       1440
Val Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu           
465                 470                 475                 480           

ctg tac aag tac aag gtg gtg aag atc gag ccc ctg ggc gtg gcc ccc       1488
Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro           
                485                 490                 495               

acc aag gcc aag cgc cgc gtg gtg cag cgc gag aag acc gga tcc tct       1536
Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Thr Gly Ser Ser           
            500                 505                 510                   

ggt ggc ggt ggc tcg ggc tcc gga gga ggt ggg tcg ggt ggc ggc gcg       1584
Gly Gly Gly Gly Ser Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ala           
        515                 520                 525                       

gcc gct gag gtg cag ctg gtg gag tct ggg gga ggc gtg gtc cag cct       1632
Ala Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro           
    530                 535                 540                           

ggg agg tcc ctg aga ctc tcc tgt gca gcc tct gga ttc acc ttc agt       1680
Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser           
545                 550                 555                 560           

agc tct ggc atg aac tgg gtc cgc cag gct cca ggc aag ggg ctg gag       1728
Ser Ser Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu           
                565                 570                 575               

tgg gtg gca att ata tca tat gat gga agt aat aaa tat tat gca gcc       1776
Trp Val Ala Ile Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Ala           
            580                 585                 590                   

tcc gtg aag ggc cga ttc acc atc tcc aga gac att tcc aag aac atc       1824
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Ile Ser Lys Asn Ile           
        595                 600                 605                       

ctg ttt ctg caa atg aac agc ctg aga gct gat gac acg gct gtg tat       1872
Leu Phe Leu Gln Met Asn Ser Leu Arg Ala Asp Asp Thr Ala Val Tyr           
    610                 615                 620                           

tac tgt aca agg ggt gta gca gtg gcg gga ccc ggc tgg aac tgg ttc       1920
Tyr Cys Thr Arg Gly Val Ala Val Ala Gly Pro Gly Trp Asn Trp Phe           
625                 630                 635                 640           

gac ccc tgg ggc cag gga acc ctg gtc acc gtc tcc tca gcg tcg acc       1968
Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr           
                645                 650                 655               

ggc ggt ggc tct ggt ggc ggc ggt tcc ggt ggc ggt gga tcc gat att       2016
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile           
            660                 665                 670                   

gtg atg act cag tct cca tcc tcc ctg tct gca tct gtg gga gac aga       2064
Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg           
        675                 680                 685                       

gtc acc atc act tgc cgg gca agt cag agg att agc agc ttt tta aat       2112
Val Thr Ile Thr Cys Arg Ala Ser Gln Arg Ile Ser Ser Phe Leu Asn           
    690                 695                 700                           

tgg tat cag cag aaa cca ggg aaa gcc cct aag gtc ctg atc tat gct       2160
Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu Ile Tyr Ala           
705                 710                 715                 720           

gca tcc agt ttg caa agt ggg gtc ccc tca agg ttc agt ggc agt gga       2208
Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly           
                725                 730                 735               

tct ggg aca gat ttc act ctc acc atc agc agt ctg caa cct gaa gac       2256
Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp           
            740                 745                 750                   

ttt gcg act tac tac tgt caa cag act tac agt ctc ccg ctc act ttc       2304
Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Tyr Ser Leu Pro Leu Thr Phe           
        755                 760                 765                       

ggc gga ggg acc aag gta gag atc aaa tga                               2334
Gly Gly Gly Thr Lys Val Glu Ile Lys                                       
    770                 775                                               


<210>  14
<211>  777
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  14

Met Arg Val Thr Glu Ile Arg Lys Ser Tyr Gln His Trp Trp Arg Trp 
1               5                   10                  15      


Gly Ile Met Leu Leu Gly Ile Leu Met Ile Cys Asn Ala Glu Glu Lys 
            20                  25                  30          


Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Thr 
        35                  40                  45              


Thr Thr Leu Phe Cys Ala Ser Asp Arg Lys Ala Tyr Asp Thr Glu Val 
    50                  55                  60                  


His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro 
65                  70                  75                  80  


Gln Glu Val Glu Leu Lys Asn Val Thr Glu Asn Phe Asn Met Trp Lys 
                85                  90                  95      


Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp 
            100                 105                 110         


Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu 
        115                 120                 125             


Asn Cys Thr Asp Leu Arg Asn Ala Thr Asn Gly Asn Asp Thr Asn Thr 
    130                 135                 140                 


Thr Ser Ser Ser Arg Gly Met Val Gly Gly Gly Glu Met Lys Asn Cys 
145                 150                 155                 160 


Ser Phe Asn Ile Thr Thr Asn Ile Arg Gly Lys Val Gln Lys Glu Tyr 
                165                 170                 175     


Ala Leu Phe Tyr Lys Leu Asp Ile Ala Pro Ile Asp Asn Asn Ser Asn 
            180                 185                 190         


Asn Arg Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln Ala 
        195                 200                 205             


Cys Pro Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro 
    210                 215                 220                 


Ala Gly Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Lys 
225                 230                 235                 240 


Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg 
                245                 250                 255     


Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu 
            260                 265                 270         


Glu Val Val Ile Arg Ser Ala Asn Phe Ala Asp Asn Ala Lys Val Ile 
        275                 280                 285             


Ile Val Gln Leu Asn Glu Ser Val Glu Ile Asn Cys Thr Arg Pro Asn 
    290                 295                 300                 


Asn Asn Thr Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr 
305                 310                 315                 320 


Thr Thr Gly Glu Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Leu 
                325                 330                 335     


Ser Arg Ala Lys Trp Asn Asp Thr Leu Asn Lys Ile Val Ile Lys Leu 
            340                 345                 350         


Arg Glu Gln Phe Gly Asn Lys Thr Ile Val Phe Lys His Ser Ser Gly 
        355                 360                 365             


Gly Asp Pro Glu Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe 
    370                 375                 380                 


Phe Tyr Cys Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp Asn Val Thr 
385                 390                 395                 400 


Glu Glu Ser Asn Asn Thr Val Glu Asn Asn Thr Ile Thr Leu Pro Cys 
                405                 410                 415     


Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met 
            420                 425                 430         


Tyr Ala Pro Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr 
        435                 440                 445             


Gly Leu Leu Leu Thr Arg Asp Gly Gly Pro Glu Asp Asn Lys Thr Glu 
    450                 455                 460                 


Val Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
465                 470                 475                 480 


Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro 
                485                 490                 495     


Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Thr Gly Ser Ser 
            500                 505                 510         


Gly Gly Gly Gly Ser Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ala 
        515                 520                 525             


Ala Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro 
    530                 535                 540                 


Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser 
545                 550                 555                 560 


Ser Ser Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
                565                 570                 575     


Trp Val Ala Ile Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Ala 
            580                 585                 590         


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Ile Ser Lys Asn Ile 
        595                 600                 605             


Leu Phe Leu Gln Met Asn Ser Leu Arg Ala Asp Asp Thr Ala Val Tyr 
    610                 615                 620                 


Tyr Cys Thr Arg Gly Val Ala Val Ala Gly Pro Gly Trp Asn Trp Phe 
625                 630                 635                 640 


Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr 
                645                 650                 655     


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile 
            660                 665                 670         


Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg 
        675                 680                 685             


Val Thr Ile Thr Cys Arg Ala Ser Gln Arg Ile Ser Ser Phe Leu Asn 
    690                 695                 700                 


Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu Ile Tyr Ala 
705                 710                 715                 720 


Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 
                725                 730                 735     


Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp 
            740                 745                 750         


Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Tyr Ser Leu Pro Leu Thr Phe 
        755                 760                 765             


Gly Gly Gly Thr Lys Val Glu Ile Lys 
    770                 775         


<210>  15
<211>  693
<212>  PRT
<213>  Human immunodeficiency virus type 1

<400>  15

Met Arg Val Thr Glu Ile Arg Lys Ser Tyr Gln His Trp Trp Arg Trp 
1               5                   10                  15      


Gly Ile Met Leu Leu Gly Ile Leu Met Ile Cys Asn Ala Glu Glu Lys 
            20                  25                  30          


Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Thr 
        35                  40                  45              


Thr Thr Leu Phe Cys Ala Ser Asp Arg Lys Ala Tyr Asp Thr Glu Val 
    50                  55                  60                  


His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro 
65                  70                  75                  80  


Gln Glu Val Glu Leu Lys Asn Val Thr Glu Asn Phe Asn Met Trp Lys 
                85                  90                  95      


Asn Asn Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp 
            100                 105                 110         


Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu 
        115                 120                 125             


Asn Cys Thr Asp Leu Arg Asn Ala Thr Asn Gly Asn Asp Thr Asn Thr 
    130                 135                 140                 


Thr Ser Ser Ser Arg Gly Met Val Gly Gly Gly Glu Met Lys Asn Cys 
145                 150                 155                 160 


Ser Phe Asn Ile Thr Thr Asn Ile Arg Gly Lys Val Gln Lys Glu Tyr 
                165                 170                 175     


Ala Leu Phe Tyr Lys Leu Asp Ile Ala Pro Ile Asp Asn Asn Ser Asn 
            180                 185                 190         


Asn Arg Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln Ala 
        195                 200                 205             


Cys Pro Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro 
    210                 215                 220                 


Ala Gly Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Lys 
225                 230                 235                 240 


Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg 
                245                 250                 255     


Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu 
            260                 265                 270         


Glu Val Val Ile Arg Ser Ala Asn Phe Ala Asp Asn Ala Lys Val Ile 
        275                 280                 285             


Ile Val Gln Leu Asn Glu Ser Val Glu Ile Asn Cys Thr Arg Pro Asn 
    290                 295                 300                 


Asn Asn Thr Arg Lys Ser Ile His Ile Gly Pro Gly Arg Ala Phe Tyr 
305                 310                 315                 320 


Thr Thr Gly Glu Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Leu 
                325                 330                 335     


Ser Arg Ala Lys Trp Asn Asp Thr Leu Asn Lys Ile Val Ile Lys Leu 
            340                 345                 350         


Arg Glu Gln Phe Gly Asn Lys Thr Ile Val Phe Lys His Ser Ser Gly 
        355                 360                 365             


Gly Asp Pro Glu Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe 
    370                 375                 380                 


Phe Tyr Cys Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp Asn Val Thr 
385                 390                 395                 400 


Glu Glu Ser Asn Asn Thr Val Glu Asn Asn Thr Ile Thr Leu Pro Cys 
                405                 410                 415     


Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met 
            420                 425                 430         


Tyr Ala Pro Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr 
        435                 440                 445             


Gly Leu Leu Leu Thr Arg Asp Gly Gly Pro Glu Asp Asn Lys Thr Glu 
    450                 455                 460                 


Val Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
465                 470                 475                 480 


Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro 
                485                 490                 495     


Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Arg Ala Val Gly 
            500                 505                 510         


Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met 
        515                 520                 525             


Gly Ala Ala Ala Met Thr Leu Thr Val Gln Ala Arg Leu Leu Leu Ser 
    530                 535                 540                 


Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln 
545                 550                 555                 560 


Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala 
                565                 570                 575     


Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly 
            580                 585                 590         


Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp 
        595                 600                 605             


Asn Ala Ser Trp Ser Asn Lys Ser Leu Asn Lys Ile Trp Asp Asn Met 
    610                 615                 620                 


Thr Trp Ile Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser Ile Ile 
625                 630                 635                 640 


Tyr Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln 
                645                 650                 655     


Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp Phe Asp 
            660                 665                 670         


Ile Thr Lys Trp Leu Trp Tyr Ile Lys Ile Phe Ile Met Ile Val Gly 
        675                 680                 685             


Gly Leu Ile Gly Leu 
    690             


<210>  16
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GSA linker sequence

<400>  16

Gly Ser Ser Gly Gly Gly Gly Ser Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ala Ala Ala 
            20      


<210>  17
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS linker sequence

<400>  17

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


