                         SEQUENCE LISTING

<110>  Merck Sharp & Dohme Corp.
       Indiana University Research Corproation
       Meehl, Michael
       Li, Pengyun
       DiMarchi, Richard D
 
<120>  O-GLYCOSYLATED CARBOXY TERMINAL PORTION (CTP) PEPTIDE-BASED 
       INSULIN AND INSULIN ANALOGUES

<130>  23093

<150>  61/732,474
<151>  2012-12-03

<160>  94    

<170>  PatentIn version 3.5

<210>  1
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CTP peptide

<400>  1

Ser Ser Ser Ser Lys Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg 
1               5                   10                  15      


Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln 
            20                  25              


<210>  2
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  2

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  3
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain peptide core sequence

<400>  3

His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Glu Arg Gly Phe Phe 
            20      


<210>  4
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  4

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  5
<211>  35
<212>  PRT
<213>  Homo sapiens

<400>  5

Arg Arg Glu Ala Glu Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly 
1               5                   10                  15      


Gly Pro Gly Ala Gly Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu 
            20                  25                  30          


Gln Lys Arg 
        35  


<210>  6
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human pre-proinsulin

<400>  6

Met Ala Leu Trp Met Arg Leu Leu Pro Leu Leu Ala Leu Leu Ala Leu 
1               5                   10                  15      


Trp Gly Pro Asp Pro Ala Ala Ala Phe Val Asn Gln His Leu Cys Gly 
            20                  25                  30          


Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe 
        35                  40                  45              


Phe Tyr Thr Pro Lys Thr Arg Arg Glu Ala Glu Asp Leu Gln Val Gly 
    50                  55                  60                  


Gln Val Glu Leu Gly Gly Gly Pro Gly Ala Gly Ser Leu Gln Pro Leu 
65                  70                  75                  80  


Ala Leu Glu Gly Ser Leu Gln Lys Arg Gly Ile Val Glu Gln Cys Cys 
                85                  90                  95      


Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn 
            100                 105                 110 


<210>  7
<211>  85
<212>  PRT
<213>  Saccharomyces cerevisiea

<400>  7

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Leu Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Glu Lys Arg 
                85  


<210>  8
<211>  934
<212>  DNA
<213>  Pichia pastoris

<400>  8
aacatccaaa gacgaaaggt tgaatgaaac ctttttgcca tccgacatcc acaggtccat       60

tctcacacat aagtgccaaa cgcaacagga ggggatacac tagcagcaga ccgttgcaaa      120

cgcaggacct ccactcctct tctcctcaac acccactttt gccatcgaaa aaccagccca      180

gttattgggc ttgattggag ctcgctcatt ccaattcctt ctattaggct actaacacca      240

tgactttatt agcctgtcta tcctggcccc cctggcgagg ttcatgtttg tttatttccg      300

aatgcaacaa gctccgcatt acacccgaac atcactccag atgagggctt tctgagtgtg      360

gggtcaaata gtttcatgtt ccccaaatgg cccaaaactg acagtttaaa cgctgtcttg      420

gaacctaata tgacaaaagc gtgatctcat ccaagatgaa ctaagtttgg ttcgttgaaa      480

tgctaacggc cagttggtca aaaagaaact tccaaaagtc ggcataccgt ttgtcttgtt      540

tggtattgat tgacgaatgc tcaaaaataa tctcattaat gcttagcgca gtctctctat      600

cgcttctgaa ccccggtgca cctgtgccga aacgcaaatg gggaaacacc cgctttttgg      660

atgattatgc attgtctcca cattgtatgc ttccaagatt ctggtgggaa tactgctgat      720

agcctaacgt tcatgatcaa aatttaactg ttctaacccc tacttgacag caatatataa      780

acagaaggaa gctgccctgt cttaaacctt tttttttatc atcattatta gcttactttc      840

ataattgcga ctggttccaa ttgacaagct tttgatttta acgactttta acgacaactt      900

gagaagatca aaaaacaact aattattcga aacg                                  934


<210>  9
<211>  260
<212>  DNA
<213>  Pichia pastoris

<400>  9
tcaagaggat gtcagaatgc catttgcctg agagatgcag gcttcatttt gatacttttt       60

tatttgtaac ctatatagta taggattttt tttgtcattt tgtttcttct cgtacgagct      120

tgctcctgat cagcctatct cgcagctgat gaatatcttg tggtaggggt ttgggaaaat      180

cattcgagtt tgatgttttt cttggtattt cccactcctc ttcagagtac agaagattaa      240

gtgagacgtt cgtttgtgca                                                  260


<210>  10
<211>  375
<212>  DNA
<213>  Streptoalloteichus hindustanus

<400>  10
atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc       60

gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt      120

gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac      180

aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag      240

gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag      300

ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc      360

gaggagcagg actga                                                       375


<210>  11
<211>  427
<212>  DNA
<213>  Saccharomyces cerevisiea

<400>  11
gatcccccac acaccatagc ttcaaaatgt ttctactcct tttttactct tccagatttt       60

ctcggactcc gcgcatcgcc gtaccacttc aaaacaccca agcacagcat actaaatttc      120

ccctctttct tcctctaggg tgtcgttaat tacccgtact aaaggtttgg aaaagaaaaa      180

agagaccgcc tcgtttcttt ttcttcgtcg aaaaaggcaa taaaaatttt tatcacgttt      240

ctttttcttg aaaatttttt tttttgattt ttttctcttt cgatgacctc ccattgatat      300

ttaagttaat aaacggtctt caatttctca agtttcagtt tcatttttct tgttctatta      360

caactttttt tacttcttgc tcattagaaa gaaagcatag caatctaatc taagttttaa      420

ttacaaa                                                                427


<210>  12
<211>  293
<212>  DNA
<213>  Saccharomyces cerevisiea

<400>  12
acaggcccct tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc       60

cctcctccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc      120

cctatttatt ttttttaata gttatgttag tattaagaac gttatttata tttcaaattt      180

ttcttttttt tctgtacaaa cgcgtgtacg catgtaacat tatactgaaa accttgcttg      240

agaaggtttt gggacgctcg aaggctttaa tttgcaagct gccggctctt aag             293


<210>  13
<211>  1793
<212>  DNA
<213>  Pichia pastoris

<400>  13
ggtttctcaa ttactatata ctactaacca tttacctgta gcgtatttct tttccctctt       60

cgcgaaagct caagggcatc ttcttgactc atgaaaaata tctggatttc ttctgacaga      120

tcatcaccct tgagcccaac tctctagcct atgagtgtaa gtgatagtca tcttgcaaca      180

gattattttg gaacgcaact aacaaagcag atacaccctt cagcagaatc ctttctggat      240

attgtgaaga atgatcgcca aagtcacagt cctgagacag ttcctaatct ttaccccatt      300

tacaagttca tccaatcaga cttcttaacg cctcatctgg cttatatcaa gcttaccaac      360

agttcagaaa ctcccagtcc aagtttcttg cttgaaagtg cgaagaatgg tgacaccgtt      420

gacaggtaca cctttatggg acattccccc agaaaaataa tcaagactgg gcctttagag      480

ggtgctgaag ttgacccctt ggtgcttctg gaaaaagaac tgaagggcac cagacaagcg      540

caacttcctg gtattcctcg tctaagtggt ggtgccatag gatacatctc gtacgattgt      600

attaagtact ttgaaccaaa aactgaaaga aaactgaaag atgttttgca acttccggaa      660

gcagctttga tgttgttcga cacgatcgtg gcttttgaca atgtttatca aagattccag      720

gtaattggaa acgtttctct atccgttgat gactcggacg aagctattct tgagaaatat      780

tataagacaa gagaagaagt ggaaaagatc agtaaagtgg tatttgacaa taaaactgtt      840

ccctactatg aacagaaaga tattattcaa ggccaaacgt tcacctctaa tattggtcag      900

gaagggtatg aaaaccatgt tcgcaagctg aaagaacata ttctgaaagg agacatcttc      960

caagctgttc cctctcaaag ggtagccagg ccgacctcat tgcacccttt caacatctat     1020

cgtcatttga gaactgtcaa tccttctcca tacatgttct atattgacta tctagacttc     1080

caagttgttg gtgcttcacc tgaattacta gttaaatccg acaacaacaa caaaatcatc     1140

acacatccta ttgctggaac tcttcccaga ggtaaaacta tcgaagagga cgacaattat     1200

gctaagcaat tgaagtcgtc tttgaaagac agggccgagc acgtcatgct ggtagatttg     1260

gccagaaatg atattaaccg tgtgtgtgag cccaccagta ccacggttga tcgtttattg     1320

actgtggaga gattttctca tgtgatgcat cttgtgtcag aagtcagtgg aacattgaga     1380

ccaaacaaga ctcgcttcga tgctttcaga tccattttcc cagcaggaac cgtctccggt     1440

gctccgaagg taagagcaat gcaactcata ggagaattgg aaggagaaaa gagaggtgtt     1500

tatgcggggg ccgtaggaca ctggtcgtac gatggaaaat cgatggacac atgtattgcc     1560

ttaagaacaa tggtcgtcaa ggacggtgtc gcttaccttc aagccggagg tggaattgtc     1620

tacgattctg acccctatga cgagtacatc gaaaccatga acaaaatgag atccaacaat     1680

aacaccatct tggaggctga gaaaatctgg accgataggt tggccagaga cgagaatcaa     1740

agtgaatccg aagaaaacga tcaatgaacg gaggacgtaa gtaggaattt atg            1793


<210>  14
<211>  3029
<212>  DNA
<213>  Saccharomyces cerevisiea

<400>  14
aggcctcgca acaacctata attgagttaa gtgcctttcc aagctaaaaa gtttgaggtt       60

ataggggctt agcatccaca cgtcacaatc tcgggtatcg agtatagtat gtagaattac      120

ggcaggaggt ttcccaatga acaaaggaca ggggcacggt gagctgtcga aggtatccat      180

tttatcatgt ttcgtttgta caagcacgac atactaagac atttaccgta tgggagttgt      240

tgtcctagcg tagttctcgc tcccccagca aagctcaaaa aagtacgtca tttagaatag      300

tttgtgagca aattaccagt cggtatgcta cgttagaaag gcccacagta ttcttctacc      360

aaaggcgtgc ctttgttgaa ctcgatccat tatgagggct tccattattc cccgcatttt      420

tattactctg aacaggaata aaaagaaaaa acccagttta ggaaattatc cgggggcgaa      480

gaaatacgcg tagcgttaat cgaccccacg tccagggttt ttccatggag gtttctggaa      540

aaactgacga ggaatgtgat tataaatccc tttatgtgat gtctaagact tttaaggtac      600

gcccgatgtt tgcctattac catcatagag acgtttcttt tcgaggaatg cttaaacgac      660

tttgtttgac aaaaatgttg cctaagggct ctatagtaaa ccatttggaa gaaagatttg      720

acgacttttt ttttttggat ttcgatccta taatccttcc tcctgaaaag aaacatataa      780

atagatatgt attattcttc aaaacattct cttgttcttg tgcttttttt ttaccatata      840

tcttactttt ttttttctct cagagaaaca agcaaaacaa aaagcttttc ttttcactaa      900

cgtatatgat gcttttgcaa gctttccttt tccttttggc tggttttgca gccaaaatat      960

ctgcatcaat gacaaacgaa actagcgata gacctttggt ccacttcaca cccaacaagg     1020

gctggatgaa tgacccaaat gggttgtggt acgatgaaaa agatgccaaa tggcatctgt     1080

actttcaata caacccaaat gacaccgtat ggggtacgcc attgttttgg ggccatgcta     1140

cttccgatga tttgactaat tgggaagatc aacccattgc tatcgctccc aagcgtaacg     1200

attcaggtgc tttctctggc tccatggtgg ttgattacaa caacacgagt gggtttttca     1260

atgatactat tgatccaaga caaagatgcg ttgcgatttg gacttataac actcctgaaa     1320

gtgaagagca atacattagc tattctcttg atggtggtta cacttttact gaataccaaa     1380

agaaccctgt tttagctgcc aactccactc aattcagaga tccaaaggtg ttctggtatg     1440

aaccttctca aaaatggatt atgacggctg ccaaatcaca agactacaaa attgaaattt     1500

actcctctga tgacttgaag tcctggaagc tagaatctgc atttgccaat gaaggtttct     1560

taggctacca atacgaatgt ccaggtttga ttgaagtccc aactgagcaa gatccttcca     1620

aatcttattg ggtcatgttt atttctatca acccaggtgc acctgctggc ggttccttca     1680

accaatattt tgttggatcc ttcaatggta ctcattttga agcgtttgac aatcaatcta     1740

gagtggtaga ttttggtaag gactactatg ccttgcaaac tttcttcaac actgacccaa     1800

cctacggttc agcattaggt attgcctggg cttcaaactg ggagtacagt gcctttgtcc     1860

caactaaccc atggagatca tccatgtctt tggtccgcaa gttttctttg aacactgaat     1920

atcaagctaa tccagagact gaattgatca atttgaaagc cgaaccaata ttgaacatta     1980

gtaatgctgg tccctggtct cgttttgcta ctaacacaac tctaactaag gccaattctt     2040

acaatgtcga tttgagcaac tcgactggta ccctagagtt tgagttggtt tacgctgtta     2100

acaccacaca aaccatatcc aaatccgtct ttgccgactt atcactttgg ttcaagggtt     2160

tagaagatcc tgaagaatat ttgagaatgg gttttgaagt cagtgcttct tccttctttt     2220

tggaccgtgg taactctaag gtcaagtttg tcaaggagaa cccatatttc acaaacagaa     2280

tgtctgtcaa caaccaacca ttcaagtctg agaacgacct aagttactat aaagtgtacg     2340

gcctactgga tcaaaacatc ttggaattgt acttcaacga tggagatgtg gtttctacaa     2400

atacctactt catgaccacc ggtaacgctc taggatctgt gaacatgacc actggtgtcg     2460

ataatttgtt ctacattgac aagttccaag taagggaagt aaaatagagg ttataaaact     2520

tattgtcttt tttatttttt tcaaaagcca ttctaaaggg ctttagctaa cgagtgacga     2580

atgtaaaact ttatgatttc aaagaatacc tccaaaccat tgaaaatgta tttttatttt     2640

tattttctcc cgaccccagt tacctggaat ttgttcttta tgtactttat ataagtataa     2700

ttctcttaaa aatttttact actttgcaat agacatcatt ttttcacgta ataaacccac     2760

aatcgtaatg tagttgcctt acactactag gatggacctt tttgccttta tctgttttgt     2820

tactgacaca atgaaaccgg gtaaagtatt agttatgtga aaatttaaaa gcattaagta     2880

gaagtatacc atattgtaaa aaaaaaaagc gttgtcttct acgtaaaagt gttctcaaaa     2940

agaagtagtg agggaaatgg ataccaagct atctgtaaca ggagctaaaa aatctcaggg     3000

aaaagcttct ggtttgggaa acggtcgac                                       3029


<210>  15
<211>  898
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 5'-Region used for knock out of PpURA5

<400>  15
atcggccttt gttgatgcaa gttttacgtg gatcatggac taaggagttt tatttggacc       60

aagttcatcg tcctagacat tacggaaagg gttctgctcc tctttttgga aactttttgg      120

aacctctgag tatgacagct tggtggattg tacccatggt atggcttcct gtgaatttct      180

attttttcta cattggattc accaatcaaa acaaattagt cgccatggct ttttggcttt      240

tgggtctatt tgtttggacc ttcttggaat atgctttgca tagatttttg ttccacttgg      300

actactatct tccagagaat caaattgcat ttaccattca tttcttattg catgggatac      360

accactattt accaatggat aaatacagat tggtgatgcc acctacactt ttcattgtac      420

tttgctaccc aatcaagacg ctcgtctttt ctgttctacc atattacatg gcttgttctg      480

gatttgcagg tggattcctg ggctatatca tgtatgatgt cactcattac gttctgcatc      540

actccaagct gcctcgttat ttccaagagt tgaagaaata tcatttggaa catcactaca      600

agaattacga gttaggcttt ggtgtcactt ccaaattctg ggacaaagtc tttgggactt      660

atctgggtcc agacgatgtg tatcaaaaga caaattagag tatttataaa gttatgtaag      720

caaatagggg ctaataggga aagaaaaatt ttggttcttt atcagagctg gctcgcgcgc      780

agtgtttttc gtgctccttt gtaatagtca tttttgacta ctgttcagat tgaaatcaca      840

ttgaagatgt cactcgaggg gtaccaaaaa aggtttttgg atgctgcagt ggcttcgc        898


<210>  16
<211>  1060
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 3'-Region used for knock out of PpURA5

<400>  16
ggtcttttca acaaagctcc attagtgagt cagctggctg aatcttatgc acaggccatc       60

attaacagca acctggagat agacgttgta tttggaccag cttataaagg tattcctttg      120

gctgctatta ccgtgttgaa gttgtacgag ctcggcggca aaaaatacga aaatgtcgga      180

tatgcgttca atagaaaaga aaagaaagac cacggagaag gtggaagcat cgttggagaa      240

agtctaaaga ataaaagagt actgattatc gatgatgtga tgactgcagg tactgctatc      300

aacgaagcat ttgctataat tggagctgaa ggtgggagag ttgaaggtag tattattgcc      360

ctagatagaa tggagactac aggagatgac tcaaatacca gtgctaccca ggctgttagt      420

cagagatatg gtacccctgt cttgagtata gtgacattgg accatattgt ggcccatttg      480

ggcgaaactt tcacagcaga cgagaaatct caaatggaaa cgtatagaaa aaagtatttg      540

cccaaataag tatgaatctg cttcgaatga atgaattaat ccaattatct tctcaccatt      600

attttcttct gtttcggagc tttgggcacg gcggcgggtg gtgcgggctc aggttccctt      660

tcataaacag atttagtact tggatgctta atagtgaatg gcgaatgcaa aggaacaatt      720

tcgttcatct ttaacccttt cactcggggt acacgttctg gaatgtaccc gccctgttgc      780

aactcaggtg gaccgggcaa ttcttgaact ttctgtaacg ttgttggatg ttcaaccaga      840

aattgtccta ccaactgtat tagtttcctt ttggtcttat attgttcatc gagatacttc      900

ccactctcct tgatagccac tctcactctt cctggattac caaaatcttg aggatgagtc      960

ttttcaggct ccaggatgca aggtatatcc aagtacctgc aagcatctaa tattgtcttt     1020

gccagggggt tctccacacc atactccttt tggcgcatgc                           1060


<210>  17
<211>  957
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the PpURA5 auxotrophic marker

<400>  17
tctagaggga cttatctggg tccagacgat gtgtatcaaa agacaaatta gagtatttat       60

aaagttatgt aagcaaatag gggctaatag ggaaagaaaa attttggttc tttatcagag      120

ctggctcgcg cgcagtgttt ttcgtgctcc tttgtaatag tcatttttga ctactgttca      180

gattgaaatc acattgaaga tgtcactgga ggggtaccaa aaaaggtttt tggatgctgc      240

agtggcttcg caggccttga agtttggaac tttcaccttg aaaagtggaa gacagtctcc      300

atacttcttt aacatgggtc ttttcaacaa agctccatta gtgagtcagc tggctgaatc      360

ttatgctcag gccatcatta acagcaacct ggagatagac gttgtatttg gaccagctta      420

taaaggtatt cctttggctg ctattaccgt gttgaagttg tacgagctgg gcggcaaaaa      480

atacgaaaat gtcggatatg cgttcaatag aaaagaaaag aaagaccacg gagaaggtgg      540

aagcatcgtt ggagaaagtc taaagaataa aagagtactg attatcgatg atgtgatgac      600

tgcaggtact gctatcaacg aagcatttgc tataattgga gctgaaggtg ggagagttga      660

aggttgtatt attgccctag atagaatgga gactacagga gatgactcaa ataccagtgc      720

tacccaggct gttagtcaga gatatggtac ccctgtcttg agtatagtga cattggacca      780

tattgtggcc catttgggcg aaactttcac agcagacgag aaatctcaaa tggaaacgta      840

tagaaaaaag tatttgccca aataagtatg aatctgcttc gaatgaatga attaatccaa      900

ttatcttctc accattattt tcttctgttt cggagctttg ggcacggcgg cggatcc         957


<210>  18
<211>  709
<212>  DNA
<213>  Escherichia coli

<400>  18
cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga       60

tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc      120

cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc      180

cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc      240

cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct      300

gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg      360

cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa      420

cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa      480

ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc      540

tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat      600

cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga      660

agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccag                  709


<210>  19
<211>  2875
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 5'-Region used for knock out of PpOCH1

<400>  19
aaaacctttt ttcctattca aacacaaggc attgcttcaa cacgtgtgcg tatccttaac       60

acagatactc catacttcta ataatgtgat agacgaatac aaagatgttc actctgtgtt      120

gtgtctacaa gcatttctta ttctgattgg ggatattcta gttacagcac taaacaactg      180

gcgatacaaa cttaaattaa ataatccgaa tctagaaaat gaacttttgg atggtccgcc      240

tgttggttgg ataaatcaat accgattaaa tggattctat tccaatgaga gagtaatcca      300

agacactctg atgtcaataa tcatttgctt gcaacaacaa acccgtcatc taatcaaagg      360

gtttgatgag gcttaccttc aattgcagat aaactcattg ctgtccactg ctgtattatg      420

tgagaatatg ggtgatgaat ctggtcttct ccactcagct aacatggctg tttgggcaaa      480

ggtggtacaa ttatacggag atcaggcaat agtgaaattg ttgaatatgg ctactggacg      540

atgcttcaag gatgtacgtc tagtaggagc cgtgggaaga ttgctggcag aaccagttgg      600

cacgtcgcaa caatccccaa gaaatgaaat aagtgaaaac gtaacgtcaa agacagcaat      660

ggagtcaata ttgataacac cactggcaga gcggttcgta cgtcgttttg gagccgatat      720

gaggctcagc gtgctaacag cacgattgac aagaagactc tcgagtgaca gtaggttgag      780

taaagtattc gcttagattc ccaaccttcg ttttattctt tcgtagacaa agaagctgca      840

tgcgaacata gggacaactt ttataaatcc aattgtcaaa ccaacgtaaa accctctggc      900

accattttca acatatattt gtgaagcagt acgcaatatc gataaatact caccgttgtt      960

tgtaacagcc ccaacttgca tacgccttct aatgacctca aatggataag ccgcagcttg     1020

tgctaacata ccagcagcac cgcccgcggt cagctgcgcc cacacatata aaggcaatct     1080

acgatcatgg gaggaattag ttttgaccgt caggtcttca agagttttga actcttcttc     1140

ttgaactgtg taacctttta aatgacggga tctaaatacg tcatggatga gatcatgtgt     1200

gtaaaaactg actccagcat atggaatcat tccaaagatt gtaggagcga acccacgata     1260

aaagtttccc aaccttgcca aagtgtctaa tgctgtgact tgaaatctgg gttcctcgtt     1320

gaagaccctg cgtactatgc ccaaaaactt tcctccacga gccctattaa cttctctatg     1380

agtttcaaat gccaaacgga cacggattag gtccaatggg taagtgaaaa acacagagca     1440

aaccccagct aatgagccgg ccagtaaccg tcttggagct gtttcataag agtcattagg     1500

gatcaataac gttctaatct gttcataaca tacaaatttt atggctgcat agggaaaaat     1560

tctcaacagg gtagccgaat gaccctgata tagacctgcg acaccatcat acccatagat     1620

ctgcctgaca gccttaaaga gcccgctaaa agacccggaa aaccgagaga actctggatt     1680

agcagtctga aaaagaatct tcactctgtc tagtggagca attaatgtct tagcggcact     1740

tcctgctact ccgccagcta ctcctgaata gatcacatac tgcaaagact gcttgtcgat     1800

gaccttgggg ttatttagct tcaagggcaa tttttgggac attttggaca caggagactc     1860

agaaacagac acagagcgtt ctgagtcctg gtgctcctga cgtaggccta gaacaggaat     1920

tattggcttt atttgtttgt ccatttcata ggcttggggt aatagataga tgacagagaa     1980

atagagaaga cctaatattt tttgttcatg gcaaatcgcg ggttcgcggt cgggtcacac     2040

acggagaagt aatgagaaga gctggtaatc tggggtaaaa gggttcaaaa gaaggtcgcc     2100

tggtagggat gcaatacaag gttgtcttgg agtttacatt gaccagatga tttggctttt     2160

tctctgttca attcacattt ttcagcgaga atcggattga cggagaaatg gcggggtgtg     2220

gggtggatag atggcagaaa tgctcgcaat caccgcgaaa gaaagacttt atggaataga     2280

actactgggt ggtgtaagga ttacatagct agtccaatgg agtccgttgg aaaggtaaga     2340

agaagctaaa accggctaag taactaggga agaatgatca gactttgatt tgatgaggtc     2400

tgaaaatact ctgctgcttt ttcagttgct ttttccctgc aacctatcat tttccttttc     2460

ataagcctgc cttttctgtt ttcacttata tgagttccgc cgagacttcc ccaaattctc     2520

tcctggaaca ttctctatcg ctctccttcc aagttgcgcc ccctggcact gcctagtaat     2580

attaccacgc gacttatatt cagttccaca atttccagtg ttcgtagcaa atatcatcag     2640

ccatggcgaa ggcagatggc agtttgctct actataatcc tcacaatcca cccagaaggt     2700

attacttcta catggctata ttcgccgttt ctgtcatttg cgttttgtac ggaccctcac     2760

aacaattatc atctccaaaa atagactatg atccattgac gctccgatca cttgatttga     2820

agactttgga agctccttca cagttgagtc caggcaccgt agaagataat cttcg          2875


<210>  20
<211>  997
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 3'-Region used for knock out of PpOCH1

<400>  20
aaagctagag taaaatagat atagcgagat tagagaatga ataccttctt ctaagcgatc       60

gtccgtcatc atagaatatc atggactgta tagttttttt tttgtacata taatgattaa      120

acggtcatcc aacatctcgt tgacagatct ctcagtacgc gaaatccctg actatcaaag      180

caagaaccga tgaagaaaaa aacaacagta acccaaacac cacaacaaac actttatctt      240

ctccccccca acaccaatca tcaaagagat gtcggaacca aacaccaaga agcaaaaact      300

aaccccatat aaaaacatcc tggtagataa tgctggtaac ccgctctcct tccatattct      360

gggctacttc acgaagtctg accggtctca gttgatcaac atgatcctcg aaatgggtgg      420

caagatcgtt ccagacctgc ctcctctggt agatggagtg ttgtttttga caggggatta      480

caagtctatt gatgaagata ccctaaagca actgggggac gttccaatat acagagactc      540

cttcatctac cagtgttttg tgcacaagac atctcttccc attgacactt tccgaattga      600

caagaacgtc gacttggctc aagatttgat caatagggcc cttcaagagt ctgtggatca      660

tgtcacttct gccagcacag ctgcagctgc tgctgttgtt gtcgctacca acggcctgtc      720

ttctaaacca gacgctcgta ctagcaaaat acagttcact cccgaagaag atcgttttat      780

tcttgacttt gttaggagaa atcctaaacg aagaaacaca catcaactgt acactgagct      840

cgctcagcac atgaaaaacc atacgaatca ttctatccgc cacagatttc gtcgtaatct      900

ttccgctcaa cttgattggg tttatgatat cgatccattg accaaccaac ctcgaaaaga      960

tgaaaacggg aactacatca aggtacaagg ccttcca                               997


<210>  21
<211>  2159
<212>  DNA
<213>  Kluyveromyces lactis

<400>  21
aaacgtaacg cctggcactc tattttctca aacttctggg acggaagagc taaatattgt       60

gttgcttgaa caaacccaaa aaaacaaaaa aatgaacaaa ctaaaactac acctaaataa      120

accgtgtgta aaacgtagta ccatattact agaaaagatc acaagtgtat cacacatgtg      180

catctcatat tacatctttt atccaatcca ttctctctat cccgtctgtt cctgtcagat      240

tctttttcca taaaaagaag aagaccccga atctcaccgg tacaatgcaa aactgctgaa      300

aaaaaaagaa agttcactgg atacgggaac agtgccagta ggcttcacca catggacaaa      360

acaattgacg ataaaataag caggtgagct tctttttcaa gtcacgatcc ctttatgtct      420

cagaaacaat atatacaagc taaacccttt tgaaccagtt ctctcttcat agttatgttc      480

acataaattg cgggaacaag actccgctgg ctgtcaggta cacgttgtaa cgttttcgtc      540

cgcccaatta ttagcacaac attggcaaaa agaaaaactg ctcgttttct ctacaggtaa      600

attacaattt ttttcagtaa ttttcgctga aaaatttaaa gggcaggaaa aaaagacgat      660

ctcgactttg catagatgca agaactgtgg tcaaaacttg aaatagtaat tttgctgtgc      720

gtgaactaat aaatatatat atatatatat atatatattt gtgtattttg tatatgtaat      780

tgtgcacgtc ttggctattg gatataagat tttcgcgggt tgatgacata gagcgtgtac      840

tactgtaata gttgtatatt caaaagctgc tgcgtggaga aagactaaaa tagataaaaa      900

gcacacattt tgacttcggt accgtcaact tagtgggaca gtcttttata tttggtgtaa      960

gctcatttct ggtactattc gaaacagaac agtgttttct gtattaccgt ccaatcgttt     1020

gtcatgagtt ttgtattgat tttgtcgtta gtgttcggag gatgttgttc caatgtgatt     1080

agtttcgagc acatggtgca aggcagcaat ataaatttgg gaaatattgt tacattcact     1140

caattcgtgt ctgtgacgct aattcagttg cccaatgctt tggacttctc tcactttccg     1200

tttaggttgc gacctagaca cattcctctt aagatccata tgttagctgt gtttttgttc     1260

tttaccagtt cagtcgccaa taacagtgtg tttaaatttg acatttccgt tccgattcat     1320

attatcatta gattttcagg taccactttg acgatgataa taggttgggc tgtttgtaat     1380

aagaggtact ccaaacttca ggtgcaatct gccatcatta tgacgcttgg tgcgattgtc     1440

gcatcattat accgtgacaa agaattttca atggacagtt taaagttgaa tacggattca     1500

gtgggtatga cccaaaaatc tatgtttggt atctttgttg tgctagtggc cactgccttg     1560

atgtcattgt tgtcgttgct caacgaatgg acgtataaca agtacgggaa acattggaaa     1620

gaaactttgt tctattcgca tttcttggct ctaccgttgt ttatgttggg gtacacaagg     1680

ctcagagacg aattcagaga cctcttaatt tcctcagact caatggatat tcctattgtt     1740

aaattaccaa ttgctacgaa acttttcatg ctaatagcaa ataacgtgac ccagttcatt     1800

tgtatcaaag gtgttaacat gctagctagt aacacggatg ctttgacact ttctgtcgtg     1860

cttctagtgc gtaaatttgt tagtctttta ctcagtgtct acatctacaa gaacgtccta     1920

tccgtgactg catacctagg gaccatcacc gtgttcctgg gagctggttt gtattcatat     1980

ggttcggtca aaactgcact gcctcgctga aacaatccac gtctgtatga tactcgtttc     2040

agaatttttt tgattttctg ccggatatgg tttctcatct ttacaatcgc attcttaatt     2100

ataccagaac gtaattcaat gatcccagtg actcgtaact cttatatgtc aatttaagc      2159


<210>  22
<211>  870
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 5'-Region used for knock out of PpBMT2

<400>  22
ggccgagcgg gcctagattt tcactacaaa tttcaaaact acgcggattt attgtctcag       60

agagcaattt ggcatttctg agcgtagcag gaggcttcat aagattgtat aggaccgtac      120

caacaaattg ccgaggcaca acacggtatg ctgtgcactt atgtggctac ttccctacaa      180

cggaatgaaa ccttcctctt tccgcttaaa cgagaaagtg tgtcgcaatt gaatgcaggt      240

gcctgtgcgc cttggtgtat tgtttttgag ggcccaattt atcaggcgcc ttttttcttg      300

gttgttttcc cttagcctca agcaaggttg gtctatttca tctccgcttc tataccgtgc      360

ctgatactgt tggatgagaa cacgactcaa cttcctgctg ctctgtattg ccagtgtttt      420

gtctgtgatt tggatcggag tcctccttac ttggaatgat aataatcttg gcggaatctc      480

cctaaacgga ggcaaggatt ctgcctatga tgatctgcta tcattgggaa gcttcaacga      540

catggaggtc gactcctatg tcaccaacat ctacgacaat gctccagtgc taggatgtac      600

ggatttgtct tatcatggat tgttgaaagt caccccaaag catgacttag cttgcgattt      660

ggagttcata agagctcaga ttttggacat tgacgtttac tccgccataa aagacttaga      720

agataaagcc ttgactgtaa aacaaaaggt tgaaaaacac tggtttacgt tttatggtag      780

ttcagtcttt ctgcccgaac acgatgtgca ttacctggtt agacgagtca tcttttcggc      840

tgaaggaaag gcgaactctc cagtaacatc                                       870


<210>  23
<211>  1733
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 3'-Region used for knock out of PpBMT2

<400>  23
ccatatgatg ggtgtttgct cactcgtatg gatcaaaatt ccatggtttc ttctgtacaa       60

cttgtacact tatttggact tttctaacgg tttttctggt gatttgagaa gtccttattt      120

tggtgttcgc agcttatccg tgattgaacc atcagaaata ctgcagctcg ttatctagtt      180

tcagaatgtg ttgtagaata caatcaattc tgagtctagt ttgggtgggt cttggcgacg      240

ggaccgttat atgcatctat gcagtgttaa ggtacataga atgaaaatgt aggggttaat      300

cgaaagcatc gttaatttca gtagaacgta gttctattcc ctacccaaat aatttgccaa      360

gaatgcttcg tatccacata cgcagtggac gtagcaaatt tcactttgga ctgtgacctc      420

aagtcgttat cttctacttg gacattgatg gtcattacgt aatccacaaa gaattggata      480

gcctctcgtt ttatctagtg cacagcctaa tagcacttaa gtaagagcaa tggacaaatt      540

tgcatagaca ttgagctaga tacgtaactc agatcttgtt cactcatggt gtactcgaag      600

tactgctgga accgttacct cttatcattt cgctactggc tcgtgaaact actggatgaa      660

aaaaaaaaaa gagctgaaag cgagatcatc ccattttgtc atcatacaaa ttcacgcttg      720

cagttttgct tcgttaacaa gacaagatgt ctttatcaaa gacccgtttt ttcttcttga      780

agaatacttc cctgttgagc acatgcaaac catatttatc tcagatttca ctcaacttgg      840

gtgcttccaa gagaagtaaa attcttccca ctgcatcaac ttccaagaaa cccgtagacc      900

agtttctctt cagccaaaag aagttgctcg ccgatcaccg cggtaacaga ggagtcagaa      960

ggtttcacac ccttccatcc cgatttcaaa gtcaaagtgc tgcgttgaac caaggttttc     1020

aggttgccaa agcccagtct gcaaaaacta gttccaaatg gcctattaat tcccataaaa     1080

gtgttggcta cgtatgtatc ggtacctcca ttctggtatt tgctattgtt gtcgttggtg     1140

ggttgactag actgaccgaa tccggtcttt ccataacgga gtggaaacct atcactggtt     1200

cggttccccc actgactgag gaagactgga agttggaatt tgaaaaatac aaacaaagcc     1260

ctgagtttca ggaactaaat tctcacataa cattggaaga gttcaagttt atattttcca     1320

tggaatgggg acatagattg ttgggaaggg tcatcggcct gtcgtttgtt cttcccacgt     1380

tttacttcat tgcccgtcga aagtgttcca aagatgttgc attgaaactg cttgcaatat     1440

gctctatgat aggattccaa ggtttcatcg gctggtggat ggtgtattcc ggattggaca     1500

aacagcaatt ggctgaacgt aactccaaac caactgtgtc tccatatcgc ttaactaccc     1560

atcttggaac tgcatttgtt atttactgtt acatgattta cacagggctt caagttttga     1620

agaactataa gatcatgaaa cagcctgaag cgtatgttca aattttcaag caaattgcgt     1680

ctccaaaatt gaaaactttc aagagactct cttcagttct attaggcctg gtg            1733


<210>  24
<211>  981
<212>  DNA
<213>  Mus musculus

<400>  24
atgtctgcca acctaaaata tctttccttg ggaattttgg tgtttcagac taccagtctg       60

gttctaacga tgcggtattc taggacttta aaagaggagg ggcctcgtta tctgtcttct      120

acagcagtgg ttgtggctga atttttgaag ataatggcct gcatcttttt agtctacaaa      180

gacagtaagt gtagtgtgag agcactgaat agagtactgc atgatgaaat tcttaataag      240

cccatggaaa ccctgaagct cgctatcccg tcagggatat atactcttca gaacaactta      300

ctctatgtgg cactgtcaaa cctagatgca gccacttacc aggttacata tcagttgaaa      360

atacttacaa cagcattatt ttctgtgtct atgcttggta aaaaattagg tgtgtaccag      420

tggctctccc tagtaattct gatggcagga gttgcttttg tacagtggcc ttcagattct      480

caagagctga actctaagga cctttcaaca ggctcacagt ttgtaggcct catggcagtt      540

ctcacagcct gtttttcaag tggctttgct ggagtttatt ttgagaaaat cttaaaagaa      600

acaaaacagt cagtatggat aaggaacatt caacttggtt tctttggaag tatatttgga      660

ttaatgggtg tatacgttta tgatggagaa ttggtctcaa agaatggatt ttttcaggga      720

tataatcaac tgacgtggat agttgttgct ctgcaggcac ttggaggcct tgtaatagct      780

gctgtcatca aatatgcaga taacatttta aaaggatttg cgacctcctt atccataata      840

ttgtcaacaa taatatctta tttttggttg caagattttg tgccaaccag tgtctttttc      900

cttggagcca tccttgtaat agcagctact ttcttgtatg gttacgatcc caaacctgca      960

ggaaatccca ctaaagcata g                                                981


<210>  25
<211>  486
<212>  DNA
<213>  Pichia pastoris

<400>  25
tttttgtaga aatgtcttgg tgtcctcgtc caatcaggta gccatctctg aaatatctgg       60

ctccgttgca actccgaacg acctgctggc aacgtaaaat tctccggggt aaaacttaaa      120

tgtggagtaa tggaaccaga aacgtctctt cccttctctc tccttccacc gcccgttacc      180

gtccctagga aattttactc tgctggagag cttcttctac ggcccccttg cagcaatgct      240

cttcccagca ttacgttgcg ggtaaaacgg aggtcgtgta cccgacctag cagcccaggg      300

atggaaaagt cccggccgtc gctggcaata atagcgggcg gacgcatgtc atgagattat      360

tggaaaccac cagaatcgaa tataaaaggc gaacaccttt cccaattttg gtttctcctg      420

acccaaagac tttaaattta atttatttgt ccctatttca atcaattgaa caactatcaa      480

aacaca                                                                 486


<210>  26
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Yps1ss

<400>  26

Met Lys Leu Lys Thr Val Arg Ser Ala Val Leu Ser Ser Leu Phe Ala 
1               5                   10                  15      


Ser Gln Val Leu Gly 
            20      


<210>  27
<211>  1128
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 5'-Region used for knock out of PpMNN4L1

<400>  27
gatctggcca ttgtgaaact tgacactaaa gacaaaactc ttagagtttc caatcactta       60

ggagacgatg tttcctacaa cgagtacgat ccctcattga tcatgagcaa tttgtatgtg      120

aaaaaagtca tcgaccttga caccttggat aaaagggctg gaggaggtgg aaccacctgt      180

gcaggcggtc tgaaagtgtt caagtacgga tctactacca aatatacatc tggtaacctg      240

aacggcgtca ggttagtata ctggaacgaa ggaaagttgc aaagctccaa atttgtggtt      300

cgatcctcta attactctca aaagcttgga ggaaacagca acgccgaatc aattgacaac      360

aatggtgtgg gttttgcctc agctggagac tcaggcgcat ggattctttc caagctacaa      420

gatgttaggg agtaccagtc attcactgaa aagctaggtg aagctacgat gagcattttc      480

gatttccacg gtcttaaaca ggagacttct actacagggc ttggggtagt tggtatgatt      540

cattcttacg acggtgagtt caaacagttt ggtttgttca ctccaatgac atctattcta      600

caaagacttc aacgagtgac caatgtagaa tggtgtgtag cgggttgcga agatggggat      660

gtggacactg aaggagaaca cgaattgagt gatttggaac aactgcatat gcatagtgat      720

tccgactagt caggcaagag agagccctca aatttacctc tctgcccctc ctcactcctt      780

ttggtacgca taattgcagt ataaagaact tgctgccagc cagtaatctt atttcatacg      840

cagttctata tagcacataa tcttgcttgt atgtatgaaa tttaccgcgt tttagttgaa      900

attgtttatg ttgtgtgcct tgcatgaaat ctctcgttag ccctatcctt acatttaact      960

ggtctcaaaa cctctaccaa ttccattgct gtacaacaat atgaggcggc attactgtag     1020

ggttggaaaa aaattgtcat tccagctaga gatcacacga cttcatcacg cttattgctc     1080

ctcattgcta aatcatttac tcttgacttc gacccagaaa agttcgcc                  1128


<210>  28
<211>  1231
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 3'-Region used for knock out of PpMNN4L1

<400>  28
gcatgtcaaa cttgaacaca acgactagat agttgttttt tctatataaa acgaaacgtt       60

atcatcttta ataatcattg aggtttaccc ttatagttcc gtattttcgt ttccaaactt      120

agtaatcttt tggaaatatc atcaaagctg gtgccaatct tcttgtttga agtttcaaac      180

tgctccacca agctacttag agactgttct aggtctgaag caacttcgaa cacagagaca      240

gctgccgccg attgttcttt tttgtgtttt tcttctggaa gaggggcatc atcttgtatg      300

tccaatgccc gtatcctttc tgagttgtcc gacacattgt ccttcgaaga gtttcctgac      360

attgggcttc ttctatccgt gtattaattt tgggttaagt tcctcgtttg catagcagtg      420

gatacctcga tttttttggc tcctatttac ctgacataat attctactat aatccaactt      480

ggacgcgtca tctatgataa ctaggctctc ctttgttcaa aggggacgtc ttcataatcc      540

actggcacga agtaagtctg caacgaggcg gcttttgcaa cagaacgata gtgtcgtttc      600

gtacttggac tatgctaaac aaaaggatct gtcaaacatt tcaaccgtgt ttcaaggcac      660

tctttacgaa ttatcgacca agaccttcct agacgaacat ttcaacatat ccaggctact      720

gcttcaaggt ggtgcaaatg ataaaggtat agatattaga tgtgtttggg acctaaaaca      780

gttcttgcct gaagattccc ttgagcaaca ggcttcaata gccaagttag agaagcagta      840

ccaaatcggt aacaaaaggg ggaagcatat aaaaccttta ctattgcgac aaaatccatc      900

cttgaaagta aagctgtttg ttcaatgtaa agcatacgaa acgaaggagg tagatcctaa      960

gatggttaga gaacttaacg ggacatactc cagctgcatc ccatattacg atcgctggaa     1020

gacttttttc atgtacgtat cgcccaccaa cctttcaaag caagctaggt atgattttga     1080

cagttctcac aatccattgg ttttcatgca acttgaaaaa acccaactca aacttcatgg     1140

ggatccatac aatgtaaatc attacgagag ggcgaggttg aaaagtttcc attgcaatca     1200

cgtcgcatca tggctactga aaggccttaa c                                    1231


<210>  29
<211>  937
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 5'-Region used for knock out of PpPNO1 and PpMNN4

<400>  29
tcattctata tgttcaagaa aagggtagtg aaaggaaaga aaaggcatat aggcgaggga       60

gagttagcta gcatacaaga taatgaagga tcaatagcgg tagttaaagt gcacaagaaa      120

agagcacctg ttgaggctga tgataaagct ccaattacat tgccacagag aaacacagta      180

acagaaatag gaggggatgc accacgagaa gagcattcag tgaacaactt tgccaaattc      240

ataaccccaa gcgctaataa gccaatgtca aagtcggcta ctaacattaa tagtacaaca      300

actatcgatt ttcaaccaga tgtttgcaag gactacaaac agacaggtta ctgcggatat      360

ggtgacactt gtaagttttt gcacctgagg gatgatttca aacagggatg gaaattagat      420

agggagtggg aaaatgtcca aaagaagaag cataatactc tcaaaggggt taaggagatc      480

caaatgttta atgaagatga gctcaaagat atcccgttta aatgcattat atgcaaagga      540

gattacaaat cacccgtgaa aacttcttgc aatcattatt tttgcgaaca atgtttcctg      600

caacggtcaa gaagaaaacc aaattgtatt atatgtggca gagacacttt aggagttgct      660

ttaccagcaa agaagttgtc ccaatttctg gctaagatac ataataatga aagtaataaa      720

gtttagtaat tgcattgcgt tgactattga ttgcattgat gtcgtgtgat actttcaccg      780

aaaaaaaaca cgaagcgcaa taggagcggt tgcatattag tccccaaagc tatttaattg      840

tgcctgaaac tgttttttaa gctcatcaag cataattgta tgcattgcga cgtaaccaac      900

gtttaggcgc agtttaatca tagcccactg ctaagcc                               937


<210>  30
<211>  1906
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 3'-Region used for knock out of PpPNO1 and PpMNN4

<400>  30
cggaggaatg caaataataa tctccttaat tacccactga taagctcaag agacgcggtt       60

tgaaaacgat ataatgaatc atttggattt tataataaac cctgacagtt tttccactgt      120

attgttttaa cactcattgg aagctgtatt gattctaaga agctagaaat caatacggcc      180

atacaaaaga tgacattgaa taagcaccgg cttttttgat tagcatatac cttaaagcat      240

gcattcatgg ctacatagtt gttaaagggc ttcttccatt atcagtataa tgaattacat      300

aatcatgcac ttatatttgc ccatctctgt tctctcactc ttgcctgggt atattctatg      360

aaattgcgta tagcgtgtct ccagttgaac cccaagcttg gcgagtttga agagaatgct      420

aaccttgcgt attccttgct tcaggaaaca ttcaaggaga aacaggtcaa gaagccaaac      480

attttgatcc ttcccgagtt agcattgact ggctacaatt ttcaaagcca gcagcggata      540

gagccttttt tggaggaaac aaccaaggga gctagtaccc aatgggctca aaaagtatcc      600

aagacgtggg attgctttac tttaatagga tacccagaaa aaagtttaga gagccctccc      660

cgtatttaca acagtgcggt acttgtatcg cctcagggaa aagtaatgaa caactacaga      720

aagtccttct tgtatgaagc tgatgaacat tggggatgtt cggaatcttc tgatgggttt      780

caaacagtag atttattaat tgaaggaaag actgtaaaga catcatttgg aatttgcatg      840

gatttgaatc cttataaatt tgaagctcca ttcacagact tcgagttcag tggccattgc      900

ttgaaaaccg gtacaagact cattttgtgc ccaatggcct ggttgtcccc tctatcgcct      960

tccattaaaa aggatcttag tgatatagag aaaagcagac ttcaaaagtt ctaccttgaa     1020

aaaatagata ccccggaatt tgacgttaat tacgaattga aaaaagatga agtattgccc     1080

acccgtatga atgaaacgtt ggaaacaatt gactttgagc cttcaaaacc ggactactct     1140

aatataaatt attggatact aaggtttttt ccctttctga ctcatgtcta taaacgagat     1200

gtgctcaaag agaatgcagt tgcagtctta tgcaaccgag ttggcattga gagtgatgtc     1260

ttgtacggag gatcaaccac gattctaaac ttcaatggta agttagcatc gacacaagag     1320

gagctggagt tgtacgggca gactaatagt ctcaacccca gtgtggaagt attgggggcc     1380

cttggcatgg gtcaacaggg aattctagta cgagacattg aattaacata atatacaata     1440

tacaataaac acaaataaag aatacaagcc tgacaaaaat tcacaaatta ttgcctagac     1500

ttgtcgttat cagcagcgac ctttttccaa tgctcaattt cacgatatgc cttttctagc     1560

tctgctttaa gcttctcatt ggaattggct aactcgttga ctgcttggtc agtgatgagt     1620

ttctccaagg tccatttctc gatgttgttg ttttcgtttt cctttaatct cttgatataa     1680

tcaacagcct tctttaatat ctgagccttg ttcgagtccc ctgttggcaa cagagcggcc     1740

agttccttta ttccgtggtt tatattttct cttctacgcc tttctacttc tttgtgattc     1800

tctttacgca tcttatgcca ttcttcagaa ccagtggctg gcttaaccga atagccagag     1860

cctgaagaag ccgcactaga agaagcagtg gcattgttga ctatgg                    1906


<210>  31
<211>  411
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 5'-Region used for knock out of  BMT1

<400>  31
catatggtga gagccgttct gcacaactag atgttttcga gcttcgcatt gtttcctgca       60

gctcgactat tgaattaaga tttccggata tctccaatct cacaaaaact tatgttgacc      120

acgtgctttc ctgaggcgag gtgttttata tgcaagctgc caaaaatgga aaacgaatgg      180

ccatttttcg cccaggcaaa ttattcgatt actgctgtca taaagacagt gttgcaaggc      240

tcacattttt ttttaggatc cgagataaag tgaatacagg acagcttatc tctatatctt      300

gtaccattcg tgaatcttaa gagttcggtt agggggactc tagttgaggg ttggcactca      360

cgtatggctg ggcgcagaaa taaaattcag gcgcagcagc acttatcgat g               411


<210>  32
<211>  692
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 3'-Region used for knock out of BMT1

<400>  32
gaattcacag ttataaataa aaacaaaaac tcaaaaagtt tgggctccac aaaataactt       60

aatttaaatt tttgtctaat aaatgaatgt aattccaaga ttatgtgatg caagcacagt      120

atgcttcagc cctatgcagc tactaatgtc aatctcgcct gcgagcgggc ctagattttc      180

actacaaatt tcaaaactac gcggatttat tgtctcagag agcaatttgg catttctgag      240

cgtagcagga ggcttcataa gattgtatag gaccgtacca acaaattgcc gaggcacaac      300

acggtatgct gtgcacttat gtggctactt ccctacaacg gaatgaaacc ttcctctttc      360

cgcttaaacg agaaagtgtg tcgcaattga atgcaggtgc ctgtgcgcct tggtgtattg      420

tttttgaggg cccaatttat caggcgcctt ttttcttggt tgttttccct tagcctcaag      480

caaggttggt ctatttcatc tccgcttcta taccgtgcct gatactgttg gatgagaaca      540

cgactcaact tcctgctgct ctgtattgcc agtgttttgt ctgtgatttg gatcggagtc      600

ctccttactt ggaatgataa taatcttggc ggaatctccc taaacggagg caaggattct      660

gcctatgatg atctgctatc attgggaagc tt                                    692


<210>  33
<211>  1043
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 5'-Region used for knock out of BMT4

<400>  33
aagcttgttc accgttggga cttttccgtg gacaatgttg actactccag gagggattcc       60

agctttctct actagctcag caataatcaa tgcagcccca ggcgcccgtt ctgatggctt      120

gatgaccgtt gtattgcctg tcactatagc caggggtagg gtccataaag gaatcatagc      180

agggaaatta aaagggcata ttgatgcaat cactcccaat ggctctcttg ccattgaagt      240

ctccatatca gcactaactt ccaagaagga ccccttcaag tctgacgtga tagagcacgc      300

ttgctctgcc acctgtagtc ctctcaaaac gtcaccttgt gcatcagcaa agactttacc      360

ttgctccaat actatgacgg aggcaattct gtcaaaattc tctctcagca attcaaccaa      420

cttgaaagca aattgctgtc tcttgatgat ggagactttt ttccaagatt gaaatgcaat      480

gtgggacgac tcaattgctt cttccagctc ctcttcggtt gattgaggaa cttttgaaac      540

cacaaaattg gtcgttgggt catgtacatc aaaccattct gtagatttag attcgacgaa      600

agcgttgttg atgaaggaaa aggttggata cggtttgtcg gtctctttgg tatggccggt      660

ggggtatgca attgcagtag aagataattg gacagccatt gttgaaggta gagaaaaggt      720

cagggaactt gggggttatt tataccattt taccccacaa ataacaactg aaaagtaccc      780

attccatagt gagaggtaac cgacggaaaa agacgggccc atgttctggg accaatagaa      840

ctgtgtaatc cattgggact aatcaacaga cgattggcaa tataatgaaa tagttcgttg      900

aaaagccacg tcagctgtct tttcattaac tttggtcgga cacaacattt tctactgttg      960

tatctgtcct actttgctta tcatctgcca cagggcaagt ggatttcctt ctcgcgcggc     1020

tgggtgaaaa cggttaacgt gaa                                             1043


<210>  34
<211>  695
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 3'-Region used for knock out of BMT4

<400>  34
gccttggggg acttcaagtc tttgctagaa actagatgag gtcaggccct cttatggttg       60

tgtcccaatt gggcaatttc actcacctaa aaagcatgac aattatttag cgaaataggt      120

agtatatttt ccctcatctc ccaagcagtt tcgtttttgc atccatatct ctcaaatgag      180

cagctacgac tcattagaac cagagtcaag taggggtgag ctcagtcatc agccttcgtt      240

tctaaaacga ttgagttctt ttgttgctac aggaagcgcc ctagggaact ttcgcacttt      300

ggaaatagat tttgatgacc aagagcggga gttgatatta gagaggctgt ccaaagtaca      360

tgggatcagg ccggccaaat tgattggtgt gactaaacca ttgtgtactt ggacactcta      420

ttacaaaagc gaagatgatt tgaagtatta caagtcccga agtgttagag gattctatcg      480

agcccagaat gaaatcatca accgttatca gcagattgat aaactcttgg aaagcggtat      540

cccattttca ttattgaaga actacgataa tgaagatgtg agagacggcg accctctgaa      600

cgtagacgaa gaaacaaatc tacttttggg gtacaataga gaaagtgaat caagggaggt      660

atttgtggcc ataatactca actctatcat taatg                                 695


<210>  35
<211>  546
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 5'-Region used for knock out of BMT3

<400>  35
gatatctccc tggggacaat atgtgttgca actgttcgtt gttggtgccc cagtccccca       60

accggtacta atcggtctat gttcccgtaa ctcatattcg gttagaacta gaacaataag      120

tgcatcattg ttcaacattg tggttcaatt gtcgaacatt gctggtgctt atatctacag      180

ggaagacgat aagcctttgt acaagagagg taacagacag ttaattggta tttctttggg      240

agtcgttgcc ctctacgttg tctccaagac atactacatt ctgagaaaca gatggaagac      300

tcaaaaatgg gagaagctta gtgaagaaga gaaagttgcc tacttggaca gagctgagaa      360

ggagaacctg ggttctaaga ggctggactt tttgttcgag agttaaactg cataattttt      420

tctaagtaaa tttcatagtt atgaaatttc tgcagcttag tgtttactgc atcgtttact      480

gcatcaccct gtaaataatg tgagcttttt tccttccatt gcttggtatc ttccttgctg      540

ctgttt                                                                 546


<210>  36
<211>  378
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 3'-Region used for knock out of BMT3

<400>  36
acaaaacagt catgtacaga actaacgcct ttaagatgca gaccactgaa aagaattggg       60

tcccattttt cttgaaagac gaccaggaat ctgtccattt tgtttactcg ttcaatcctc      120

tgagagtact caactgcagt cttgataacg gtgcatgtga tgttctattt gagttaccac      180

atgattttgg catgtcttcc gagctacgtg gtgccactcc tatgctcaat cttcctcagg      240

caatcccgat ggcagacgac aaagaaattt gggtttcatt cccaagaacg agaatatcag      300

attgcgggtg ttctgaaaca atgtacaggc caatgttaat gctttttgtt agagaaggaa      360

caaacttttt tgctgagc                                                    378


<210>  37
<211>  2577
<212>  DNA
<213>  Leishmania major

<400>  37
atgggtaaaa gaaagggaaa ctccttggga gattctggtt ctgctgctac tgcttccaga       60

gaggcttctg ctcaagctga agatgctgct tcccagacta agactgcttc tccacctgct      120

aaggttatct tgttgccaaa gactttgact gacgagaagg acttcatcgg tatcttccca      180

tttccattct ggccagttca cttcgttttg actgttgttg ctttgttcgt tttggctgct      240

tcctgtttcc aggctttcac tgttagaatg atctccgttc aaatctacgg ttacttgatc      300

cacgaatttg acccatggtt caactacaga gctgctgagt acatgtctac tcacggatgg      360

agtgcttttt tctcctggtt cgattacatg tcctggtatc cattgggtag accagttggt      420

tctactactt acccaggatt gcagttgact gctgttgcta tccatagagc tttggctgct      480

gctggaatgc caatgtcctt gaacaatgtt tgtgttttga tgccagcttg gtttggtgct      540

atcgctactg ctactttggc tttctgtact tacgaggctt ctggttctac tgttgctgct      600

gctgcagctg ctttgtcctt ctccattatc cctgctcact tgatgagatc catggctggt      660

gagttcgaca acgagtgtat tgctgttgct gctatgttgt tgactttcta ctgttgggtt      720

cgttccttga gaactagatc ctcctggcca atcggtgttt tgacaggtgt tgcttacggt      780

tacatggctg ctgcttgggg aggttacatc ttcgttttga acatggttgc tatgcacgct      840

ggtatctctt ctatggttga ctgggctaga aacacttaca acccatcctt gttgagagct      900

tacactttgt tctacgttgt tggtactgct atcgctgttt gtgttccacc agttggaatg      960

tctccattca agtccttgga gcagttggga gctttgttgg ttttggtttt cttgtgtgga     1020

ttgcaagttt gtgaggtttt gagagctaga gctggtgttg aagttagatc cagagctaat     1080

ttcaagatca gagttagagt tttctccgtt atggctggtg ttgctgcttt ggctatctct     1140

gttttggctc caactggtta ctttggtcca ttgtctgtta gagttagagc tttgtttgtt     1200

gagcacacta gaactggtaa cccattggtt gactccgttg ctgaacatca accagcttct     1260

ccagaggcta tgtgggcttt cttgcatgtt tgtggtgtta cttggggatt gggttccatt     1320

gttttggctg tttccacttt cgttcactac tccccatcta aggttttctg gttgttgaac     1380

tccggtgctg tttactactt ctccactaga atggctagat tgttgttgtt gtccggtcca     1440

gctgcttgtt tgtccactgg tatcttcgtt ggtactatct tggaggctgc tgttcaattg     1500

tctttctggg actccgatgc tactaaggct aagaagcagc aaaagcaggc tcaaagacac     1560

caaagaggtg ctggtaaagg ttctggtaga gatgacgcta agaacgctac tactgctaga     1620

gctttctgtg acgttttcgc tggttcttct ttggcttggg gtcacagaat ggttttgtcc     1680

attgctatgt gggctttggt tactactact gctgtttcct tcttctcctc cgaatttgct     1740

tctcactcca ctaagttcgc tgaacaatcc tccaacccaa tgatcgtttt cgctgctgtt     1800

gttcagaaca gagctactgg aaagccaatg aacttgttgg ttgacgacta cttgaaggct     1860

tacgagtggt tgagagactc tactccagag gacgctagag ttttggcttg gtgggactac     1920

ggttaccaaa tcactggtat cggtaacaga acttccttgg ctgatggtaa cacttggaac     1980

cacgagcaca ttgctactat cggaaagatg ttgacttccc cagttgttga agctcactcc     2040

cttgttagac acatggctga ctacgttttg atttgggctg gtcaatctgg tgacttgatg     2100

aagtctccac acatggctag aatcggtaac tctgtttacc acgacatttg tccagatgac     2160

ccattgtgtc agcaattcgg tttccacaga aacgattact ccagaccaac tccaatgatg     2220

agagcttcct tgttgtacaa cttgcacgag gctggaaaaa gaaagggtgt taaggttaac     2280

ccatctttgt tccaagaggt ttactcctcc aagtacggac ttgttagaat cttcaaggtt     2340

atgaacgttt ccgctgagtc taagaagtgg gttgcagacc cagctaacag agtttgtcac     2400

ccacctggtt cttggatttg tcctggtcaa tacccacctg ctaaagaaat ccaagagatg     2460

ttggctcaca gagttccatt cgaccaggtt acaaacgctg acagaaagaa caatgttggt     2520

tcctaccaag aggaatacat gagaagaatg agagagtccg agaacagaag ataatag        2577


<210>  38
<211>  1215
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  38
atgtcagaag atcaaaaaag tgaaaattcc gtaccttcta aggttaatat ggtgaatcgc       60

accgatatac tgactacgat caagtcattg tcatggcttg acttgatgtt gccatttact      120

ataattctct ccataatcat tgcagtaata atttctgtct atgtgccttc ttcccgtcac      180

acttttgacg ctgaaggtca tcccaatcta atgggagtgt ccattccttt gactgttggt      240

atgattgtaa tgatgattcc cccgatctgc aaagtttcct gggagtctat tcacaagtac      300

ttctacagga gctatataag gaagcaacta gccctctcgt tatttttgaa ttgggtcatc      360

ggtcctttgt tgatgacagc attggcgtgg atggcgctat tcgattataa ggaataccgt      420

caaggcatta ttatgatcgg agtagctaga tgcattgcca tggtgctaat ttggaatcag      480

attgctggag gagacaatga tctctgcgtc gtgcttgtta ttacaaactc gcttttacag      540

atggtattat atgcaccatt gcagatattt tactgttatg ttatttctca tgaccacctg      600

aatacttcaa atagggtatt attcgaagag gttgcaaagt ctgtcggagt ttttctcggc      660

ataccactgg gaattggcat tatcatacgt ttgggaagtc ttaccatagc tggtaaaagt      720

aattatgaaa aatacatttt gagatttatt tctccatggg caatgatcgg atttcattac      780

actttatttg ttatttttat tagtagaggt tatcaattta tccacgaaat tggttctgca      840

atattgtgct ttgtcccatt ggtgctttac ttctttattg catggttttt gaccttcgca      900

ttaatgaggt acttatcaat atctaggagt gatacacaaa gagaatgtag ctgtgaccaa      960

gaactacttt taaagagggt ctggggaaga aagtcttgtg aagctagctt ttctattacg     1020

atgacgcaat gtttcactat ggcttcaaat aattttgaac tatccctggc aattgctatt     1080

tccttatatg gtaacaatag caagcaagca atagctgcaa catttgggcc gttgctagaa     1140

gttccaattt tattgatttt ggcaatagtc gcgagaatcc ttaaaccata ttatatatgg     1200

aacaatagaa attaa                                                      1215


<210>  39
<211>  600
<212>  DNA
<213>  Pichia pastoris

<400>  39
gttcttcgct tggtcttgta tctccttaca ctgtatcttc ccatttgcgt ttaggtggtt       60

atcaaaaact aaaaggaaaa atttcagatg tttatctcta aggttttttc tttttacagt      120

ataacacgtg atgcgtcacg tggtactaga ttacgtaagt tattttggtc cggtgggtaa      180

gtgggtaaga atagaaagca tgaaggttta caaaaacgca gtcacgaatt attgctactt      240

cgagcttgga accaccccaa agattatatt gtactgatgc actaccttct cgattttgct      300

cctccaagaa cctacgaaaa acatttcttg agccttttca acctagacta cacatcaagt      360

tatttaaggt atgttccgtt aacatgtaag aaaaggagag gatagatcgt ttatggggta      420

cgtcgcctga ttcaagcgtg accattcgaa gaataggcct tcgaaagctg aataaagcaa      480

atgtcagttg cgattggtat gctgacaaat tagcataaaa agcaatagac tttctaacca      540

cctgtttttt tccttttact ttatttatat tttgccaccg tactaacaag ttcagacaaa      600


<210>  40
<211>  1144
<212>  DNA
<213>  Pichia pastoris

<400>  40
caaatgcaag aggacattag aaatgtgttt ggtaagaaca tgaagccgga ggcatacaaa       60

cgattcacag atttgaagga ggaaaacaaa ctgcatccac cggaagtgcc agcagccgtg      120

tatgccaacc ttgctctcaa aggcattcct acggatctga gtgggaaata tctgagattc      180

acagacccac tattggaaca gtaccaaacc tagtttggcc gatccatgat tatgtaatgc      240

atatagtttt tgtcgatgct cacccgtttc gagtctgtct cgtatcgtct tacgtataag      300

ttcaagcatg tttaccaggt ctgttagaaa ctcctttgtg agggcaggac ctattcgtct      360

cggtcccgtt gtttctaaga gactgtacag ccaagcgcag aatggtggca ttaaccataa      420

gaggattctg atcggacttg gtctattggc tattggaacc accctttacg ggacaaccaa      480

ccctaccaag actcctattg catttgtgga accagccacg gaaagagcgt ttaaggacgg      540

agacgtctct gtgatttttg ttctcggagg tccaggagct ggaaaaggta cccaatgtgc      600

caaactagtg agtaattacg gatttgttca cctgtcagct ggagacttgt tacgtgcaga      660

acagaagagg gaggggtcta agtatggaga gatgatttcc cagtatatca gagatggact      720

gatagtacct caagaggtca ccattgcgct cttggagcag gccatgaagg aaaacttcga      780

gaaagggaag acacggttct tgattgatgg attccctcgt aagatggacc aggccaaaac      840

ttttgaggaa aaagtcgcaa agtccaaggt gacacttttc tttgattgtc ccgaatcagt      900

gctccttgag agattactta aaagaggaca gacaagcgga agagaggatg ataatgcgga      960

gagtatcaaa aaaagattca aaacattcgt ggaaacttcg atgcctgtgg tggactattt     1020

cgggaagcaa ggacgcgttt tgaaggtatc ttgtgaccac cctgtggatc aagtgtattc     1080

acaggttgtg tcggtgctaa aagagaaggg gatctttgcc gataacgaga cggagaataa     1140

ataa                                                                  1144


<210>  41
<211>  255
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encodes Saccharomyces cerevisiae mating factor pre-propeptide

<400>  41
atgagatttc cttcaatttt tactgcagtt ttattcgcag catcctccgc attagctgct       60

ccagtcaaca ctacaacaga agatgaaacg gcacaaattc cggctgaagc tgtcatcggt      120

tactcagatt tagaagggga tttcgatgtt gctgttttgc cattttccaa cagcacaaat      180

aacgggttat tgtttataaa tactactatt gccagcattg ctgctaaaga agaaggggta      240

tctctcgaga aaagg                                                       255


<210>  42
<211>  237
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encodes SCI (C peptide CTP)

<400>  42
tttgttaacc aacatttgtg tggttctcac cttgttgaag ctttgtacct tgtctgcgga       60

gagagaggat ttttctatac tcctaagaca tcttcctcaa gtaaagcccc acctccatca      120

ttgccttctc catccagact tcctggtcca tccgataccc ctattttgcc acaaggaatc      180

gtcgaacagt gttgcacttc aatttgtagt ttgtaccagc ttgagaacta ttgcaat         237


<210>  43
<211>  79
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SCI (C peptide CTP)

<400>  43

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Ser Ser 
            20                  25                  30          


Ser Ser Lys Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg Leu Pro 
        35                  40                  45              


Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln Gly Ile Val Glu Gln Cys 
    50                  55                  60                  


Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn 
65                  70                  75                  


<210>  44
<211>  240
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encodes SCI (C peptide CTP+K)

<400>  44
tttgttaacc aacatttgtg tggttctcac cttgttgaag ctttgtacct tgtctgcgga       60

gagagaggat ttttctatac tcctaagaca tcttcctcaa gtagagcccc acctccatca      120

ttgccttctc catccagact tcctggtcca tccgataccc ctattttgcc acaaaaagga      180

atcgtcgaac agtgttgcac ttcaatttgt agtttgtacc agcttgagaa ctattgcaat      240


<210>  45
<211>  80
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SCI (C peptide CTP+K)

<400>  45

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Ser Ser 
            20                  25                  30          


Ser Ser Arg Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg Leu Pro 
        35                  40                  45              


Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln Lys Gly Ile Val Glu Gln 
    50                  55                  60                  


Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn 
65                  70                  75                  80  


<210>  46
<211>  246
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encodes SCI (N-spacer CTP)

<400>  46
tcttcctcaa gtagagcacc acctccatca ttgccttctc catccagact tcctggtcca       60

tccgataccc ctattttgcc acaatttgtt aaccagcatt tgtgtggatc tcaccttgtt      120

gaagctttgt accttgtctg cggagagaga ggatttttct atactcctaa gacagctgcc      180

aaaggtattg tcgaacaatg ttgcacttca atctgtagtt tgtaccagct tgagaactat      240

tgcaat                                                                 246


<210>  47
<211>  82
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SCI (N-spacer CTP)

<400>  47

Ser Ser Ser Ser Arg Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg 
1               5                   10                  15      


Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln Phe Val Asn Gln 
            20                  25                  30          


His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly 
        35                  40                  45              


Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Ala Ala Lys Gly Ile Val 
    50                  55                  60                  


Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr 
65                  70                  75                  80  


Cys Asn 
        


<210>  48
<211>  249
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encodes SCI (N-spacer CTP +K)

<400>  48
tcttcctcaa gtagagcacc acctccatca ttgccttctc catccagact tcctggtcca       60

tccgataccc ctattttgcc acaaaagttt gttaaccagc atttgtgtgg atctcacctt      120

gttgaagctt tgtaccttgt ctgcggagag agaggatttt tctatactcc taagacagct      180

gccaaaggta ttgtcgaaca atgttgcact tcaatctgta gtttgtacca gcttgagaac      240

tattgcaat                                                              249


<210>  49
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SCI (N-spacer CTP +K)

<400>  49

Ser Ser Ser Ser Arg Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg 
1               5                   10                  15      


Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln Lys Phe Val Asn 
            20                  25                  30          


Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys 
        35                  40                  45              


Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Ala Ala Lys Gly Ile 
    50                  55                  60                  


Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn 
65                  70                  75                  80  


Tyr Cys Asn 
            


<210>  50
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encodes SCI (N-spacer CTP; C-peptide CTP)

<400>  50
tcttcctcaa gtaaggctcc acctccatca ttgccatctc cttccagact tccaggtcct       60

agtgatacac caattttgcc tcaatttgtt aaccagcatt tgtgtggatc tcaccttgtt      120

gaagctttgt accttgtctg cggagagaga ggatttttct atactccaaa gacatcttcc      180

tcaagtaaag cccctccacc ttccttgcca tcacctagta gacttccagg tccttctgac      240

accccaattt tgcctcaagg aatcgtcgaa cagtgttgca cttctatctg ttccttgtac      300

caacttgaga actattgcaa t                                                321


<210>  51
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SCI (N-spacer CTP; C-peptide CTP)

<400>  51

Ser Ser Ser Ser Lys Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg 
1               5                   10                  15      


Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln Phe Val Asn Gln 
            20                  25                  30          


His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly 
        35                  40                  45              


Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Ser Ser Ser Ser Lys Ala 
    50                  55                  60                  


Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg Leu Pro Gly Pro Ser Asp 
65                  70                  75                  80  


Thr Pro Ile Leu Pro Gln Gly Ile Val Glu Gln Cys Cys Thr Ser Ile 
                85                  90                  95      


Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn 
            100                 105         


<210>  52
<211>  324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encodes SCI (N-spacer CTP; C-peptide CTP+K)

<400>  52
tcttcttctt ctagagcccc acctccatca ttgccatctc cttccagact tccaggtcct       60

agtgatacac caattttgcc tcaatttgtt aaccagcatt tgtgtggatc tcaccttgtt      120

gaagctttgt accttgtctg cggagagaga ggatttttct atactccaaa gacatcttcc      180

tcaagtagag cccctccacc ttccttgcca tcacctagta gacttccagg tccttctgac      240

accccaattt tgcctcaaaa aggaatcgtc gaacagtgtt gcacttctat ctgttccttg      300

taccaacttg agaactattg caat                                             324


<210>  53
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SCI (N-spacer CTP; C-peptide CTP+K)

<400>  53

Ser Ser Ser Ser Arg Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg 
1               5                   10                  15      


Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln Phe Val Asn Gln 
            20                  25                  30          


His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly 
        35                  40                  45              


Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Ser Ser Ser Ser Arg Ala 
    50                  55                  60                  


Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg Leu Pro Gly Pro Ser Asp 
65                  70                  75                  80  


Thr Pro Ile Leu Pro Gln Lys Gly Ile Val Glu Gln Cys Cys Thr Ser 
                85                  90                  95      


Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn 
            100                 105             


<210>  54
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' MylI leader sequence

<400>  54
gagtcctctt                                                              10


<210>  55
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' stop-stop-FseI sequence

<400>  55
taatagggcc ggcc                                                         14


<210>  56
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  56

Gly Ala Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10          


<210>  57
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  57

Gly Ala Gly Ser Ser Ser Arg Arg Ala 
1               5                   


<210>  58
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  58

Gly Ala Gly Ser Ser Ser Arg Arg 
1               5               


<210>  59
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  59

Gly Gly Gly Pro Arg Arg 
1               5       


<210>  60
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  60

Gly Gly Gly Pro Gly Ala Gly 
1               5           


<210>  61
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  61

Gly Gly Gly Gly Gly Lys Arg 
1               5           


<210>  62
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  62

Gly Gly Gly Pro Gly Lys Arg 
1               5           


<210>  63
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  63

Val Gly Leu Ser Ser Gly Gln 
1               5           


<210>  64
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  64

Thr Gly Leu Gly Ser Gly Arg 
1               5           


<210>  65
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  65

Arg Arg Gly Pro Gly Gly Gly 
1               5           


<210>  66
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  66

Arg Arg Gly Gly Gly Gly Gly 
1               5           


<210>  67
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  67

Gly Gly Ala Pro Gly Asp Val Lys Arg 
1               5                   


<210>  68
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  68

Arg Arg Ala Pro Gly Asp Val Gly Gly 
1               5                   


<210>  69
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  69

Gly Gly Tyr Pro Gly Asp Val Leu Arg 
1               5                   


<210>  70
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  70

Arg Arg Tyr Pro Gly Asp Val Gly Gly 
1               5                   


<210>  71
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  71

Gly Gly His Pro Gly Asp Val Arg 
1               5               


<210>  72
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Connecting peptide

<400>  72

Arg Arg His Pro Gly Asp Val Gly Gly 
1               5                   


<210>  73
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Insulin lispro B-chain

<400>  73

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Lys Pro Thr 
            20                  25                  30  


<210>  74
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Insulin aspart B-chain

<400>  74

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Lys Thr 
            20                  25                  30  


<210>  75
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Insulin glulisine

<400>  75

Phe Val Lys Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Glu Thr 
            20                  25                  30  


<210>  76
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Insulin degludec, desB30, Lys B29 conjugated to hexadecandioic 
       acid

       (B29N(epsilon)-omega-carboxypentadecanoyl-gamma-L-glutamyl desB30
       human insulin)

<400>  76

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys 
            20                  25                  


<210>  77
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Insulin glargine A-chain

<400>  77

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Gly 
            20      


<210>  78
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Insulin glargine B-chain

<400>  78

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Arg Arg 
            20                  25                  30      


<210>  79
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ArgA0

<400>  79

Arg Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Asn 
            20          


<210>  80
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ArgA0A21

<400>  80

Arg Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln 
1               5                   10                  15      


Leu Glu Asn Tyr Cys Gly 
            20          


<210>  81
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Insulin degludec, desB30, Lys B29 conjugated to myristic acid

<400>  81

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys 
            20                  25                  


<210>  82
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TA57 pro

<400>  82

Gln Pro Ile Asp Asp Thr Glu Ser Gln Thr Thr Ser Val Asn Leu Met 
1               5                   10                  15      


Ala Asp Asp Thr Glu Ser Ala Phe Ala Thr Gln Thr Asn Ser Gly Gly 
            20                  25                  30          


Leu Asp Val Val Gly Leu Ile Ser Met Ala Lys Arg 
        35                  40                  


<210>  83
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal spacer

<400>  83

Glu Glu Gly Glu Pro Lys 
1               5       


<210>  84
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C peptide "AAK"

<400>  84

Ala Ala Lys 
1           


<210>  85
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Saccharomyces cerevisiae mating factor pre-signal peptide (DNA)

<400>  85
atgagattcc catccatctt cactgctgtt ttgttcgctg cttcttctgc tttggct          57


<210>  86
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Saccharomyces cerevisiae mating factor pre-signal peptide 
       (protein)

<400>  86

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala 
            


<210>  87
<211>  1494
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encodes Tricoderma reesei ?1,2-mannosidase catalytic domain

<400>  87
cgcgccggat ctcccaaccc tacgagggcg gcagcagtca aggccgcatt ccagacgtcg       60

tggaacgctt accaccattt tgcctttccc catgacgacc tccacccggt cagcaacagc      120

tttgatgatg agagaaacgg ctggggctcg tcggcaatcg atggcttgga cacggctatc      180

ctcatggggg atgccgacat tgtgaacacg atccttcagt atgtaccgca gatcaacttc      240

accacgactg cggttgccaa ccaaggcatc tccgtgttcg agaccaacat tcggtacctc      300

ggtggcctgc tttctgccta tgacctgttg cgaggtcctt tcagctcctt ggcgacaaac      360

cagaccctgg taaacagcct tctgaggcag gctcaaacac tggccaacgg cctcaaggtt      420

gcgttcacca ctcccagcgg tgtcccggac cctaccgtct tcttcaaccc tactgtccgg      480

agaagtggtg catctagcaa caacgtcgct gaaattggaa gcctggtgct cgagtggaca      540

cggttgagcg acctgacggg aaacccgcag tatgcccagc ttgcgcagaa gggcgagtcg      600

tatctcctga atccaaaggg aagcccggag gcatggcctg gcctgattgg aacgtttgtc      660

agcacgagca acggtacctt tcaggatagc agcggcagct ggtccggcct catggacagc      720

ttctacgagt acctgatcaa gatgtacctg tacgacccgg ttgcgtttgc acactacaag      780

gatcgctggg tccttgctgc cgactcgacc attgcgcatc tcgcctctca cccgtcgacg      840

cgcaaggact tgaccttttt gtcttcgtac aacggacagt ctacgtcgcc aaactcagga      900

catttggcca gttttgccgg tggcaacttc atcttgggag gcattctcct gaacgagcaa      960

aagtacattg actttggaat caagcttgcc agctcgtact ttgccacgta caaccagacg     1020

gcttctggaa tcggccccga aggcttcgcg tgggtggaca gcgtgacggg cgccggcggc     1080

tcgccgccct cgtcccagtc cgggttctac tcgtcggcag gattctgggt gacggcaccg     1140

tattacatcc tgcggccgga gacgctggag agcttgtact acgcataccg cgtcacgggc     1200

gactccaagt ggcaggacct ggcgtgggaa gcgttcagtg ccattgagga cgcatgccgc     1260

gccggcagcg cgtactcgtc catcaacgac gtgacgcagg ccaacggcgg gggtgcctct     1320

gacgatatgg agagcttctg gtttgccgag gcgctcaagt atgcgtacct gatctttgcg     1380

gaggagtcgg atgtgcaggt gcaggccaac ggcgggaaca aatttgtctt taacacggag     1440

gcgcacccct ttagcatccg ttcatcatca cgacggggcg gccaccttgc ttaa           1494


<210>  88
<211>  1231
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 5'-region to knock into the PpPRO1 locus

<400>  88
gaagggccat cgaattgtca tcgtctcctc aggtgccatc gctgtgggca tgaagagagt       60

caacatgaag cggaaaccaa aaaagttaca gcaagtgcag gcattggctg ctataggaca      120

aggccgtttg ataggacttt gggacgacct tttccgtcag ttgaatcagc ctattgcgca      180

gattttactg actagaacgg atttggtcga ttacacccag tttaagaacg ctgaaaatac      240

attggaacag cttattaaaa tgggtattat tcctattgtc aatgagaatg acaccctatc      300

cattcaagaa atcaaatttg gtgacaatga caccttatcc gccataacag ctggtatgtg      360

tcatgcagac tacctgtttt tggtgactga tgtggactgt ctttacacgg ataaccctcg      420

tacgaatccg gacgctgagc caatcgtgtt agttagaaat atgaggaatc taaacgtcaa      480

taccgaaagt ggaggttccg ccgtaggaac aggaggaatg acaactaaat tgatcgcagc      540

tgatttgggt gtatctgcag gtgttacaac gattatttgc aaaagtgaac atcccgagca      600

gattttggac attgtagagt acagtatccg tgctgataga gtcgaaaatg aggctaaata      660

tctggtcatc aacgaagagg aaactgtgga acaatttcaa gagatcaatc ggtcagaact      720

gagggagttg aacaagctgg acattccttt gcatacacgt ttcgttggcc acagttttaa      780

tgctgttaat aacaaagagt tttggttact ccatggacta aaggccaacg gagccattat      840

cattgatcca ggttgttata aggctatcac tagaaaaaac aaagctggta ttcttccagc      900

tggaattatt tccgtagagg gtaatttcca tgaatacgag tgtgttgatg ttaaggtagg      960

actaagagat ccagatgacc cacattcact agaccccaat gaagaacttt acgtcgttgg     1020

ccgtgcccgt tgtaattacc ccagcaatca aatcaacaaa attaagggtc tacaaagctc     1080

gcagatcgag caggttctag gttacgctga cggtgagtat gttgttcaca gggacaactt     1140

ggctttccca gtatttgccg atccagaact gttggatgtt gttgagagta ccctgtctga     1200

acaggagaga gaatccaaac caaataaata g                                    1231


<210>  89
<211>  1425
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 3'-region to knock into the PpPRO1 locus

<400>  89
aatttcacat atgctgcttg attatgtaat tataccttgc gttcgatggc atcgatttcc       60

tcttctgtca atcgcgcatc gcattaaaag tatacttttt tttttttcct atagtactat      120

tcgccttatt ataaactttg ctagtatgag ttctaccccc aagaaagagc ctgatttgac      180

tcctaagaag agtcagcctc caaagaatag tctcggtggg ggtaaaggct ttagtgagga      240

gggtttctcc caaggggact tcagcgctaa gcatatacta aatcgtcgcc ctaacaccga      300

aggctcttct gtggcttcga acgtcatcag ttcgtcatca ttgcaaaggt taccatcctc      360

tggatctgga agcgttgctg tgggaagtgt gttgggatct tcgccattaa ctctttctgg      420

agggttccac gggcttgatc caaccaagaa taaaatagac gttccaaagt cgaaacagtc      480

aaggagacaa agtgttcttt ctgacatgat ttccacttct catgcagcta gaaatgatca      540

ctcagagcag cagttacaaa ctggacaaca atcagaacaa aaagaagaag atggtagtcg      600

atcttctttt tctgtttctt cccccgcaag agatatccgg cacccagatg tactgaaaac      660

tgtcgagaaa catcttgcca atgacagcga gatcgactca tctttacaac ttcaaggtgg      720

agatgtcact agaggcattt atcaatgggt aactggagaa agtagtcaaa aagataaccc      780

gcctttgaaa cgagcaaata gttttaatga tttttcttct gtgcatggtg acgaggtagg      840

caaggcagat gctgaccacg atcgtgaaag cgtattcgac gaggatgata tctccattga      900

tgatatcaaa gttccgggag ggatgcgtcg aagtttttta ttacaaaagc atagagacca      960

acaactttct ggactgaata aaacggctca ccaaccaaaa caacttacta aacctaattt     1020

cttcacgaac aactttatag agtttttggc attgtatggg cattttgcag gtgaagattt     1080

ggaggaagac gaagatgaag atttagacag tggttccgaa tcagtcgcag tcagtgatag     1140

tgagggagaa ttcagtgagg ctgacaacaa tttgttgtat gatgaagagt ctctcctatt     1200

agcacctagt acctccaact atgcgagatc aagaatagga agtattcgta ctcctactta     1260

tggatctttc agttcaaatg ttggttcttc gtctattcat cagcagttaa tgaaaagtca     1320

aatcccgaag ctgaagaaac gtggacagca caagcataaa acacaatcaa aaatacgctc     1380

gaagaagcaa actaccaccg taaaagcagt gttgctgcta ttaaa                     1425


<210>  90
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Portion of human insulin A-chain

<400>  90

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys 
            20  


<210>  91
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTP+K

<400>  91

Ser Ser Ser Ser Lys Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg 
1               5                   10                  15      


Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln Lys 
            20                  25                  


<210>  92
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTP+R

<400>  92

Ser Ser Ser Ser Lys Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg 
1               5                   10                  15      


Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln Arg 
            20                  25                  


<210>  93
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTP+KR

<400>  93

Ser Ser Ser Ser Lys Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg 
1               5                   10                  15      


Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln Lys Arg 
            20                  25                  30  


<210>  94
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTP+RR

<400>  94

Ser Ser Ser Ser Lys Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg 
1               5                   10                  15      


Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln Arg Arg 
            20                  25                  30  


