                         SEQUENCE LISTING

<110>  MicroCoat Biotechnologie GmbH
 
<120>  Novel method for recombinant production of horseshoe crab 
       Factor C protein

<130>  PCT82127FZ210pau

<140>  not yet assigned
<141>  herewith

<150>  US 61/734,002
<151>  2012-12-06

<150>  EP12195742.7
<151>  2012-12-05

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  2982
<212>  DNA
<213>  Tachypleus tridentatus

<400>  1
agaggagtag atctgggctt gtgtgatgaa acgaggttcg agtgtaagtg tggagatcca       60

ggctatgtgt tcaacgtccc tatgaaacaa tgcacgtact tctatcgatg gaggccttat      120

tgtaaaccat gtgatgacct ggaggctaag gacatttgtc caaagtacaa acgatgtcaa      180

gagtgtaagg ctggtcttga tagttgtgtt acttgtccac ctaacaaata tggtacttgg      240

tgtagcggtg aatgtcaatg taagaatgga ggtatctgtg accagaggac aggagcttgt      300

acctgtcgtg acagatatga aggagcgcac tgtgaaattc tcaaaggttg tcctcttctt      360

ccatcggatt ctcaagttca ggaagtcaga aacccaccag ataatcccca aactattgac      420

tacagctgtt caccagggtt caagcttaaa ggcgtggcac gaattagctg tctcccaaat      480

ggacagtgga gtagctttcc acccaaatgt attcgagaat gtgccaaggt ttcatctcca      540

gaacacggga aagtgaatgc tcctagtggc aatatgatag aaggggctac tttacggttc      600

tcatgtgata gtccctacta cttgattggt caagaaacat taacctgcca gggtaatggt      660

cagtggagtg gacaaatacc acaatgtaag aagttggtct tctgtcctga ccttgatcct      720

gtaaaccatg ctgaacacca ggttaaaatt ggtgtggaac aaaaatatgg tcagtttcct      780

caaggcactg aagtgaccta tacgtgttcg ggtaactact tcttgatggg ttttaacacc      840

ttaaaatgta accctgatgg gtcctggtca ggatcacagc catcctgtgt taaagtggca      900

gacagagagg tcgactgtga cagtaaagct gtagacttct tggatgatgt tggtgaacct      960

gtcaggatcc actgtcctgc tggctgttct ttgacagctg gtactgtgtg gggtacagcc     1020

atataccacg aactttcctc agtgtgtcgt gcagccatcc atgctggcaa gcttccaaac     1080

tctggagggg cggtgcatgt agtgaacaat ggcccctact cggactttct gggtagtgac     1140

ctgaatggga taaaatcgga agagttgaag tctcttgccc gcagttttcg atttgattat     1200

gtcagttcat ccacagcagg tagatcagga tgtcctgatg gatggtttga ggtagaagag     1260

aactgtgtgt acgttacatc aaaacagaga gcctgggaaa gagctcaagg tgtgtgtacc     1320

aatatggctg ctcgtcttgc tgtgctagac aaagatctaa ttccgagttc cttgactgag     1380

actctacgag ggaaagggtt aacaaccaca tggataggat tgcacagact agatgctgag     1440

aagccctttg tttgggagct aatggatcgt agtaatgtgg ttctgaatga taacctaaca     1500

ttctgggcct ctggcgaacc tggaaatgaa actaactgtg tatatctgga catccgagat     1560

cagctgcagc ctgtgtggaa aaccaagtca tgttttcagc cctcaagctt tgcttgcatg     1620

atggatttgt cagacagaaa taaagccaaa tgcgatgacc ctggaccact ggaaaatgga     1680

cacgccacac ttcatggaca aagtattgat gggttctatg ctggttcttc tataaggtac     1740

agctgtgagg ttctccacta cctcagtgga actgagaccg taacttgtac aacaaatggc     1800

acatggagtg ctcctaaacc tcgatgtatc aaagtcatca cctgccaaaa ccctcctgta     1860

ccatcatatg gttctgtgga aatcaaaccc ccaagtcgga caaactcgat cagtcgtgtt     1920

gggtcacctt tcttgaggtt gccacggtta cccctcccat tagccagagc agccaaacct     1980

cctccaaaac ctagatcctc acaaccctct actgtggact tggcttctaa agttaaacta     2040

cctgaaggtc attaccgggt agggtctcga gccatttaca cgtgcgagtc gagatactac     2100

gaactacttg gatctcaagg cagaagatgt gactctaatg gaaactggag tggtcggccc     2160

gctagctgta ttccagtttg tggacggtca gactctcctc gttctccttt catctggaat     2220

gggaattcta cagaaatagg tcagtggccg tggcaggcag gaatctctcg atggcttgca     2280

gaccacaata tgtggtttct ccagtgtgga ggatccctat tgaatgagaa atggatcgtc     2340

actgctgccc actgtgtcac ctactctgct actgctgaga taattgatcc cagtcagttt     2400

aaaatctatc tgggcaagta ctaccgtgat gacagtagag acgatgacta cgtacaagta     2460

agagaggctc tcgagatcca cgtaaatcct aactacgacc ccggcaatct caactttgac     2520

atagccctaa ttcaactgaa aactcctgtt actttgacaa cacgagtcca accaatctgt     2580

ctgcctactg acatcacaac aagagaacac ttgaaggagg gaacattagc agtggtgaca     2640

ggttggggtt tgaatgaaaa caacacatat tcagagatga ttcaacaagc tgtgctacct     2700

gttgttgcag caagcacctg tgaagagggg tacaaggaag cagacttacc actgacagta     2760

acagagaaca tgttctgtgc aggttacaag aagggacgtt atgatgcctg cagtggggac     2820

agtggaggac cattagtgtt tgctgatgat tcccgtaccg aaaggcggtg ggtcttggaa     2880

gggattgtca gctggggcag tcccagtgga tgtggcaagg ctaaccagta tgggggcttc     2940

actaaagtta acgtttttct atcatggatt aggcagttca tt                        2982


<210>  2
<211>  994
<212>  PRT
<213>  Tachypleus tridentatus

<400>  2

Arg Gly Val Asp Leu Gly Leu Cys Asp Glu Thr Arg Phe Glu Cys Lys 
1               5                   10                  15      


Cys Gly Asp Pro Gly Tyr Val Phe Asn Val Pro Met Lys Gln Cys Thr 
            20                  25                  30          


Tyr Phe Tyr Arg Trp Arg Pro Tyr Cys Lys Pro Cys Asp Asp Leu Glu 
        35                  40                  45              


Ala Lys Asp Ile Cys Pro Lys Tyr Lys Arg Cys Gln Glu Cys Lys Ala 
    50                  55                  60                  


Gly Leu Asp Ser Cys Val Thr Cys Pro Pro Asn Lys Tyr Gly Thr Trp 
65                  70                  75                  80  


Cys Ser Gly Glu Cys Gln Cys Lys Asn Gly Gly Ile Cys Asp Gln Arg 
                85                  90                  95      


Thr Gly Ala Cys Thr Cys Arg Asp Arg Tyr Glu Gly Ala His Cys Glu 
            100                 105                 110         


Ile Leu Lys Gly Cys Pro Leu Leu Pro Ser Asp Ser Gln Val Gln Glu 
        115                 120                 125             


Val Arg Asn Pro Pro Asp Asn Pro Gln Thr Ile Asp Tyr Ser Cys Ser 
    130                 135                 140                 


Pro Gly Phe Lys Leu Lys Gly Val Ala Arg Ile Ser Cys Leu Pro Asn 
145                 150                 155                 160 


Gly Gln Trp Ser Ser Phe Pro Pro Lys Cys Ile Arg Glu Cys Ala Lys 
                165                 170                 175     


Val Ser Ser Pro Glu His Gly Lys Val Asn Ala Pro Ser Gly Asn Met 
            180                 185                 190         


Ile Glu Gly Ala Thr Leu Arg Phe Ser Cys Asp Ser Pro Tyr Tyr Leu 
        195                 200                 205             


Ile Gly Gln Glu Thr Leu Thr Cys Gln Gly Asn Gly Gln Trp Ser Gly 
    210                 215                 220                 


Gln Ile Pro Gln Cys Lys Lys Leu Val Phe Cys Pro Asp Leu Asp Pro 
225                 230                 235                 240 


Val Asn His Ala Glu His Gln Val Lys Ile Gly Val Glu Gln Lys Tyr 
                245                 250                 255     


Gly Gln Phe Pro Gln Gly Thr Glu Val Thr Tyr Thr Cys Ser Gly Asn 
            260                 265                 270         


Tyr Phe Leu Met Gly Phe Asn Thr Leu Lys Cys Asn Pro Asp Gly Ser 
        275                 280                 285             


Trp Ser Gly Ser Gln Pro Ser Cys Val Lys Val Ala Asp Arg Glu Val 
    290                 295                 300                 


Asp Cys Asp Ser Lys Ala Val Asp Phe Leu Asp Asp Val Gly Glu Pro 
305                 310                 315                 320 


Val Arg Ile His Cys Pro Ala Gly Cys Ser Leu Thr Ala Gly Thr Val 
                325                 330                 335     


Trp Gly Thr Ala Ile Tyr His Glu Leu Ser Ser Val Cys Arg Ala Ala 
            340                 345                 350         


Ile His Ala Gly Lys Leu Pro Asn Ser Gly Gly Ala Val His Val Val 
        355                 360                 365             


Asn Asn Gly Pro Tyr Ser Asp Phe Leu Gly Ser Asp Leu Asn Gly Ile 
    370                 375                 380                 


Lys Ser Glu Glu Leu Lys Ser Leu Ala Arg Ser Phe Arg Phe Asp Tyr 
385                 390                 395                 400 


Val Ser Ser Ser Thr Ala Gly Arg Ser Gly Cys Pro Asp Gly Trp Phe 
                405                 410                 415     


Glu Val Glu Glu Asn Cys Val Tyr Val Thr Ser Lys Gln Arg Ala Trp 
            420                 425                 430         


Glu Arg Ala Gln Gly Val Cys Thr Asn Met Ala Ala Arg Leu Ala Val 
        435                 440                 445             


Leu Asp Lys Asp Leu Ile Pro Ser Ser Leu Thr Glu Thr Leu Arg Gly 
    450                 455                 460                 


Lys Gly Leu Thr Thr Thr Trp Ile Gly Leu His Arg Leu Asp Ala Glu 
465                 470                 475                 480 


Lys Pro Phe Val Trp Glu Leu Met Asp Arg Ser Asn Val Val Leu Asn 
                485                 490                 495     


Asp Asn Leu Thr Phe Trp Ala Ser Gly Glu Pro Gly Asn Glu Thr Asn 
            500                 505                 510         


Cys Val Tyr Leu Asp Ile Arg Asp Gln Leu Gln Pro Val Trp Lys Thr 
        515                 520                 525             


Lys Ser Cys Phe Gln Pro Ser Ser Phe Ala Cys Met Met Asp Leu Ser 
    530                 535                 540                 


Asp Arg Asn Lys Ala Lys Cys Asp Asp Pro Gly Pro Leu Glu Asn Gly 
545                 550                 555                 560 


His Ala Thr Leu His Gly Gln Ser Ile Asp Gly Phe Tyr Ala Gly Ser 
                565                 570                 575     


Ser Ile Arg Tyr Ser Cys Glu Val Leu His Tyr Leu Ser Gly Thr Glu 
            580                 585                 590         


Thr Val Thr Cys Thr Thr Asn Gly Thr Trp Ser Ala Pro Lys Pro Arg 
        595                 600                 605             


Cys Ile Lys Val Ile Thr Cys Gln Asn Pro Pro Val Pro Ser Tyr Gly 
    610                 615                 620                 


Ser Val Glu Ile Lys Pro Pro Ser Arg Thr Asn Ser Ile Ser Arg Val 
625                 630                 635                 640 


Gly Ser Pro Phe Leu Arg Leu Pro Arg Leu Pro Leu Pro Leu Ala Arg 
                645                 650                 655     


Ala Ala Lys Pro Pro Pro Lys Pro Arg Ser Ser Gln Pro Ser Thr Val 
            660                 665                 670         


Asp Leu Ala Ser Lys Val Lys Leu Pro Glu Gly His Tyr Arg Val Gly 
        675                 680                 685             


Ser Arg Ala Ile Tyr Thr Cys Glu Ser Arg Tyr Tyr Glu Leu Leu Gly 
    690                 695                 700                 


Ser Gln Gly Arg Arg Cys Asp Ser Asn Gly Asn Trp Ser Gly Arg Pro 
705                 710                 715                 720 


Ala Ser Cys Ile Pro Val Cys Gly Arg Ser Asp Ser Pro Arg Ser Pro 
                725                 730                 735     


Phe Ile Trp Asn Gly Asn Ser Thr Glu Ile Gly Gln Trp Pro Trp Gln 
            740                 745                 750         


Ala Gly Ile Ser Arg Trp Leu Ala Asp His Asn Met Trp Phe Leu Gln 
        755                 760                 765             


Cys Gly Gly Ser Leu Leu Asn Glu Lys Trp Ile Val Thr Ala Ala His 
    770                 775                 780                 


Cys Val Thr Tyr Ser Ala Thr Ala Glu Ile Ile Asp Pro Ser Gln Phe 
785                 790                 795                 800 


Lys Ile Tyr Leu Gly Lys Tyr Tyr Arg Asp Asp Ser Arg Asp Asp Asp 
                805                 810                 815     


Tyr Val Gln Val Arg Glu Ala Leu Glu Ile His Val Asn Pro Asn Tyr 
            820                 825                 830         


Asp Pro Gly Asn Leu Asn Phe Asp Ile Ala Leu Ile Gln Leu Lys Thr 
        835                 840                 845             


Pro Val Thr Leu Thr Thr Arg Val Gln Pro Ile Cys Leu Pro Thr Asp 
    850                 855                 860                 


Ile Thr Thr Arg Glu His Leu Lys Glu Gly Thr Leu Ala Val Val Thr 
865                 870                 875                 880 


Gly Trp Gly Leu Asn Glu Asn Asn Thr Tyr Ser Glu Met Ile Gln Gln 
                885                 890                 895     


Ala Val Leu Pro Val Val Ala Ala Ser Thr Cys Glu Glu Gly Tyr Lys 
            900                 905                 910         


Glu Ala Asp Leu Pro Leu Thr Val Thr Glu Asn Met Phe Cys Ala Gly 
        915                 920                 925             


Tyr Lys Lys Gly Arg Tyr Asp Ala Cys Ser Gly Asp Ser Gly Gly Pro 
    930                 935                 940                 


Leu Val Phe Ala Asp Asp Ser Arg Thr Glu Arg Arg Trp Val Leu Glu 
945                 950                 955                 960 


Gly Ile Val Ser Trp Gly Ser Pro Ser Gly Cys Gly Lys Ala Asn Gln 
                965                 970                 975     


Tyr Gly Gly Phe Thr Lys Val Asn Val Phe Leu Ser Trp Ile Arg Gln 
            980                 985                 990         


Phe Ile 
        


<210>  3
<211>  2982
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of synthetic Factor C gene (nucleotide 
       sequence of Factor C from Tachypleus tridentatus codon 
       optimized for expression in Leishmania)

<400>  3
aggggtgtgg acctgggcct gtgcgacgag acccgcttcg agtgcaagtg cggcgacccg       60

ggctacgtgt tcaacgtgcc gatgaagcag tgcacgtact tctaccgctg gcgcccgtac      120

tgcaagccgt gcgacgacct ggaggcgaag gacatctgcc cgaagtacaa gcgctgccag      180

gagtgcaagg cgggcctgga cagctgcgtg acgtgcccgc cgaacaagta cggcacgtgg      240

tgcagcggcg agtgccagtg caagaacggt ggcatctgcg atcagcgcac gggcgcgtgc      300

acatgccgcg atcgctacga gggcgcgcac tgcgagatcc tgaagggctg cccgctgctg      360

ccgagcgaca gccaggtgca ggaggtgcgc aacccgccgg acaacccgca gacgatcgac      420

tactcgtgca gccccggctt caagctcaag ggcgtggcgc gcatcagctg cctcccgaac      480

ggtcagtggt cgagcttccc gccgaagtgc atccgcgagt gcgcgaaggt gagcagcccg      540

gagcacggca aggtgaacgc gccgagcggc aacatgatcg agggcgcgac gctgcgcttc      600

agctgcgaca gcccgtacta cctgatcggc caggagaccc tgacctgcca gggcaacggc      660

cagtggagcg gccagatccc gcagtgcaag aagctggtct tctgcccgga cctggacccg      720

gtgaaccacg cggagcacca ggtgaagatc ggcgtggagc agaagtacgg ccagttcccg      780

cagggcacgg aggtgacgta cacgtgcagc ggcaactact tcctgatggg cttcaacacg      840

ctgaagtgca acccggacgg tagctggtcg ggcagccagc cgtcctgcgt gaaggtggcg      900

gaccgcgagg tggactgcga cagcaaggcg gtggacttcc tggacgacgt gggcgagccg      960

gtgcgcatcc actgcccggc tggctgcagc ctgacagcgg gcacggtgtg gggcacggcg     1020

atctaccacg agctgtcgag cgtgtgccgc gctgcgatcc acgcgggcaa gctgccgaac     1080

agcggcggtg cggtgcacgt ggtgaacaac ggcccgtaca gcgacttcct gggcagcgac     1140

ctgaacggca tcaagagcga ggagctgaag agcctggccc gcagcttccg cttcgactac     1200

gtgagcagca gcacggctgg tcgcagcggc tgcccggacg gctggttcga ggtggaggag     1260

aactgcgtct acgtcacgag caagcagcgc gcgtgggagc gcgcgcaggg cgtgtgcacg     1320

aacatggcgg ctcgcctggc cgtgctggac aaggacctga tcccgagcag cctgacggag     1380

accctgcgcg gcaagggcct gacgacgacg tggatcggcc tgcaccgcct ggacgcggag     1440

aagccgttcg tgtgggagct gatggaccgc agcaacgtgg tgctgaacga caacctgacg     1500

ttctgggcga gcggcgagcc gggcaacgag accaactgcg tgtacctgga catccgcgac     1560

cagctgcagc cggtgtggaa gacgaagagc tgcttccagc cgagctcctt cgcgtgcatg     1620

atggacctga gcgaccgcaa caaggcgaag tgcgacgacc cgggtccgct ggagaacggc     1680

cacgcgacgc tgcacggcca gagcatcgac ggcttctacg cgggcagcag catccgctac     1740

agctgcgagg tgctgcacta cctgagcggc acggagaccg tgacgtgcac gacgaacggc     1800

acgtggtccg cgccgaagcc gcgctgcatc aaggtgatca cgtgccagaa cccgccggtg     1860

ccgagctacg gcagcgtgga gatcaagccg ccgtcgcgca cgaactcgat tagccgcgtg     1920

ggctcgccgt tcctgcgtct gccacgcctc ccactgccgc tggctcgtgc ggccaagccg     1980

ccaccgaagc cacgcagcag ccagccgagc acggtggacc tggccagcaa ggtgaagctg     2040

ccggagggcc actaccgcgt gggctcgcgc gcgatctaca cgtgcgagag ccgctactac     2100

gagctgctgg gcagccaggg tcgtcgctgc gacagcaacg gcaactggag cggtcgcccg     2160

gctagctgca tcccggtgtg cggtcgcagc gactccccgc gctcgccgtt catctggaac     2220

ggcaacagca cggagatcgg tcagtggccc tggcaggcgg gcatcagccg ctggctggcc     2280

gaccacaaca tgtggttcct ccagtgcggc ggcagcctgc tgaacgagaa gtggattgtg     2340

acggcggctc actgcgtgac gtactcggcg acggccgaga tcatcgaccc gagccagttc     2400

aagatctacc tgggcaagta ctaccgcgac gacagccgcg acgacgacta cgtgcaggtg     2460

cgcgaggcgc tggagatcca cgtgaacccg aactacgacc cgggcaacct gaacttcgat     2520

atcgcgctga tccagctcaa gacgccggtg acgctgacga cgcgcgtgca gccgatctgc     2580

ctgccgacgg acatcacgac gcgcgagcac ctgaaggagg gcacgctggc cgtcgtgacg     2640

ggctggggcc tgaacgagaa caacacgtac agcgagatga tccagcaggc ggtgctgccg     2700

gtggtggcgg cgagcacgtg cgaggagggc tacaaggagg cggacctgcc gctgacggtg     2760

acggagaaca tgttctgcgc gggctacaag aagggccgct acgacgcctg cagcggtgac     2820

agcggcggtc cgctggtgtt cgcggacgac agccgcacgg agcgccgctg ggtgctggag     2880

ggcatcgtga gctggggcag cccgagcggt tgcggcaagg cgaaccagta cggcggcttc     2940

acgaaggtga acgtgttcct cagctggatc cgccagttta tc                        2982


<210>  4
<211>  994
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of synthetic Factor C protein (amino acid 
       sequence encoded by the codon optimized nucleotide sequence of 
       SEQ ID NO: 3)

<400>  4

Arg Gly Val Asp Leu Gly Leu Cys Asp Glu Thr Arg Phe Glu Cys Lys 
1               5                   10                  15      


Cys Gly Asp Pro Gly Tyr Val Phe Asn Val Pro Met Lys Gln Cys Thr 
            20                  25                  30          


Tyr Phe Tyr Arg Trp Arg Pro Tyr Cys Lys Pro Cys Asp Asp Leu Glu 
        35                  40                  45              


Ala Lys Asp Ile Cys Pro Lys Tyr Lys Arg Cys Gln Glu Cys Lys Ala 
    50                  55                  60                  


Gly Leu Asp Ser Cys Val Thr Cys Pro Pro Asn Lys Tyr Gly Thr Trp 
65                  70                  75                  80  


Cys Ser Gly Glu Cys Gln Cys Lys Asn Gly Gly Ile Cys Asp Gln Arg 
                85                  90                  95      


Thr Gly Ala Cys Thr Cys Arg Asp Arg Tyr Glu Gly Ala His Cys Glu 
            100                 105                 110         


Ile Leu Lys Gly Cys Pro Leu Leu Pro Ser Asp Ser Gln Val Gln Glu 
        115                 120                 125             


Val Arg Asn Pro Pro Asp Asn Pro Gln Thr Ile Asp Tyr Ser Cys Ser 
    130                 135                 140                 


Pro Gly Phe Lys Leu Lys Gly Val Ala Arg Ile Ser Cys Leu Pro Asn 
145                 150                 155                 160 


Gly Gln Trp Ser Ser Phe Pro Pro Lys Cys Ile Arg Glu Cys Ala Lys 
                165                 170                 175     


Val Ser Ser Pro Glu His Gly Lys Val Asn Ala Pro Ser Gly Asn Met 
            180                 185                 190         


Ile Glu Gly Ala Thr Leu Arg Phe Ser Cys Asp Ser Pro Tyr Tyr Leu 
        195                 200                 205             


Ile Gly Gln Glu Thr Leu Thr Cys Gln Gly Asn Gly Gln Trp Ser Gly 
    210                 215                 220                 


Gln Ile Pro Gln Cys Lys Lys Leu Val Phe Cys Pro Asp Leu Asp Pro 
225                 230                 235                 240 


Val Asn His Ala Glu His Gln Val Lys Ile Gly Val Glu Gln Lys Tyr 
                245                 250                 255     


Gly Gln Phe Pro Gln Gly Thr Glu Val Thr Tyr Thr Cys Ser Gly Asn 
            260                 265                 270         


Tyr Phe Leu Met Gly Phe Asn Thr Leu Lys Cys Asn Pro Asp Gly Ser 
        275                 280                 285             


Trp Ser Gly Ser Gln Pro Ser Cys Val Lys Val Ala Asp Arg Glu Val 
    290                 295                 300                 


Asp Cys Asp Ser Lys Ala Val Asp Phe Leu Asp Asp Val Gly Glu Pro 
305                 310                 315                 320 


Val Arg Ile His Cys Pro Ala Gly Cys Ser Leu Thr Ala Gly Thr Val 
                325                 330                 335     


Trp Gly Thr Ala Ile Tyr His Glu Leu Ser Ser Val Cys Arg Ala Ala 
            340                 345                 350         


Ile His Ala Gly Lys Leu Pro Asn Ser Gly Gly Ala Val His Val Val 
        355                 360                 365             


Asn Asn Gly Pro Tyr Ser Asp Phe Leu Gly Ser Asp Leu Asn Gly Ile 
    370                 375                 380                 


Lys Ser Glu Glu Leu Lys Ser Leu Ala Arg Ser Phe Arg Phe Asp Tyr 
385                 390                 395                 400 


Val Ser Ser Ser Thr Ala Gly Arg Ser Gly Cys Pro Asp Gly Trp Phe 
                405                 410                 415     


Glu Val Glu Glu Asn Cys Val Tyr Val Thr Ser Lys Gln Arg Ala Trp 
            420                 425                 430         


Glu Arg Ala Gln Gly Val Cys Thr Asn Met Ala Ala Arg Leu Ala Val 
        435                 440                 445             


Leu Asp Lys Asp Leu Ile Pro Ser Ser Leu Thr Glu Thr Leu Arg Gly 
    450                 455                 460                 


Lys Gly Leu Thr Thr Thr Trp Ile Gly Leu His Arg Leu Asp Ala Glu 
465                 470                 475                 480 


Lys Pro Phe Val Trp Glu Leu Met Asp Arg Ser Asn Val Val Leu Asn 
                485                 490                 495     


Asp Asn Leu Thr Phe Trp Ala Ser Gly Glu Pro Gly Asn Glu Thr Asn 
            500                 505                 510         


Cys Val Tyr Leu Asp Ile Arg Asp Gln Leu Gln Pro Val Trp Lys Thr 
        515                 520                 525             


Lys Ser Cys Phe Gln Pro Ser Ser Phe Ala Cys Met Met Asp Leu Ser 
    530                 535                 540                 


Asp Arg Asn Lys Ala Lys Cys Asp Asp Pro Gly Pro Leu Glu Asn Gly 
545                 550                 555                 560 


His Ala Thr Leu His Gly Gln Ser Ile Asp Gly Phe Tyr Ala Gly Ser 
                565                 570                 575     


Ser Ile Arg Tyr Ser Cys Glu Val Leu His Tyr Leu Ser Gly Thr Glu 
            580                 585                 590         


Thr Val Thr Cys Thr Thr Asn Gly Thr Trp Ser Ala Pro Lys Pro Arg 
        595                 600                 605             


Cys Ile Lys Val Ile Thr Cys Gln Asn Pro Pro Val Pro Ser Tyr Gly 
    610                 615                 620                 


Ser Val Glu Ile Lys Pro Pro Ser Arg Thr Asn Ser Ile Ser Arg Val 
625                 630                 635                 640 


Gly Ser Pro Phe Leu Arg Leu Pro Arg Leu Pro Leu Pro Leu Ala Arg 
                645                 650                 655     


Ala Ala Lys Pro Pro Pro Lys Pro Arg Ser Ser Gln Pro Ser Thr Val 
            660                 665                 670         


Asp Leu Ala Ser Lys Val Lys Leu Pro Glu Gly His Tyr Arg Val Gly 
        675                 680                 685             


Ser Arg Ala Ile Tyr Thr Cys Glu Ser Arg Tyr Tyr Glu Leu Leu Gly 
    690                 695                 700                 


Ser Gln Gly Arg Arg Cys Asp Ser Asn Gly Asn Trp Ser Gly Arg Pro 
705                 710                 715                 720 


Ala Ser Cys Ile Pro Val Cys Gly Arg Ser Asp Ser Pro Arg Ser Pro 
                725                 730                 735     


Phe Ile Trp Asn Gly Asn Ser Thr Glu Ile Gly Gln Trp Pro Trp Gln 
            740                 745                 750         


Ala Gly Ile Ser Arg Trp Leu Ala Asp His Asn Met Trp Phe Leu Gln 
        755                 760                 765             


Cys Gly Gly Ser Leu Leu Asn Glu Lys Trp Ile Val Thr Ala Ala His 
    770                 775                 780                 


Cys Val Thr Tyr Ser Ala Thr Ala Glu Ile Ile Asp Pro Ser Gln Phe 
785                 790                 795                 800 


Lys Ile Tyr Leu Gly Lys Tyr Tyr Arg Asp Asp Ser Arg Asp Asp Asp 
                805                 810                 815     


Tyr Val Gln Val Arg Glu Ala Leu Glu Ile His Val Asn Pro Asn Tyr 
            820                 825                 830         


Asp Pro Gly Asn Leu Asn Phe Asp Ile Ala Leu Ile Gln Leu Lys Thr 
        835                 840                 845             


Pro Val Thr Leu Thr Thr Arg Val Gln Pro Ile Cys Leu Pro Thr Asp 
    850                 855                 860                 


Ile Thr Thr Arg Glu His Leu Lys Glu Gly Thr Leu Ala Val Val Thr 
865                 870                 875                 880 


Gly Trp Gly Leu Asn Glu Asn Asn Thr Tyr Ser Glu Met Ile Gln Gln 
                885                 890                 895     


Ala Val Leu Pro Val Val Ala Ala Ser Thr Cys Glu Glu Gly Tyr Lys 
            900                 905                 910         


Glu Ala Asp Leu Pro Leu Thr Val Thr Glu Asn Met Phe Cys Ala Gly 
        915                 920                 925             


Tyr Lys Lys Gly Arg Tyr Asp Ala Cys Ser Gly Asp Ser Gly Gly Pro 
    930                 935                 940                 


Leu Val Phe Ala Asp Asp Ser Arg Thr Glu Arg Arg Trp Val Leu Glu 
945                 950                 955                 960 


Gly Ile Val Ser Trp Gly Ser Pro Ser Gly Cys Gly Lys Ala Asn Gln 
                965                 970                 975     


Tyr Gly Gly Phe Thr Lys Val Asn Val Phe Leu Ser Trp Ile Arg Gln 
            980                 985                 990         


Phe Ile 
       
 

<210>  5
<211>  23
<212>  PRT
<213>  Leishmania tarentolae
 

<400>  5

Met Ala Ser Arg Leu Val Arg Val Leu Ala Ala Ala Met Leu Val Ala 
1               5                   10                  15      


Ala Ala Val Ser Val Asp Ala 
            20              






