                         SEQUENCE LISTING

<110>  Universitaet des Saarlandes
 
<120>  Progranulin as marker for autoimmune disorders

<130>  SAR14603PCT

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  593
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc
<222>  (1)..(593)
<223>  Progranulin

<400>  1

Met Trp Thr Leu Val Ser Trp Val Ala Leu Thr Ala Gly Leu Val Ala 
1               5                   10                  15      


Gly Thr Arg Cys Pro Asp Gly Gln Phe Cys Pro Val Ala Cys Cys Leu 
            20                  25                  30          


Asp Pro Gly Gly Ala Ser Tyr Ser Cys Cys Arg Pro Leu Leu Asp Lys 
        35                  40                  45              


Trp Pro Thr Thr Leu Ser Arg His Leu Gly Gly Pro Cys Gln Val Asp 
    50                  55                  60                  


Ala His Cys Ser Ala Gly His Ser Cys Ile Phe Thr Val Ser Gly Thr 
65                  70                  75                  80  


Ser Ser Cys Cys Pro Phe Pro Glu Ala Val Ala Cys Gly Asp Gly His 
                85                  90                  95      


His Cys Cys Pro Arg Gly Phe His Cys Ser Ala Asp Gly Arg Ser Cys 
            100                 105                 110         


Phe Gln Arg Ser Gly Asn Asn Ser Val Gly Ala Ile Gln Cys Pro Asp 
        115                 120                 125             


Ser Gln Phe Glu Cys Pro Asp Phe Ser Thr Cys Cys Val Met Val Asp 
    130                 135                 140                 


Gly Ser Trp Gly Cys Cys Pro Met Pro Gln Ala Ser Cys Cys Glu Asp 
145                 150                 155                 160 


Arg Val His Cys Cys Pro His Gly Ala Phe Cys Asp Leu Val His Thr 
                165                 170                 175     


Arg Cys Ile Thr Pro Thr Gly Thr His Pro Leu Ala Lys Lys Leu Pro 
            180                 185                 190         


Ala Gln Arg Thr Asn Arg Ala Val Ala Leu Ser Ser Ser Val Met Cys 
        195                 200                 205             


Pro Asp Ala Arg Ser Arg Cys Pro Asp Gly Ser Thr Cys Cys Glu Leu 
    210                 215                 220                 


Pro Ser Gly Lys Tyr Gly Cys Cys Pro Met Pro Asn Ala Thr Cys Cys 
225                 230                 235                 240 


Ser Asp His Leu His Cys Cys Pro Gln Asp Thr Val Cys Asp Leu Ile 
                245                 250                 255     


Gln Ser Lys Cys Leu Ser Lys Glu Asn Ala Thr Thr Asp Leu Leu Thr 
            260                 265                 270         


Lys Leu Pro Ala His Thr Val Gly Asp Val Lys Cys Asp Met Glu Val 
        275                 280                 285             


Ser Cys Pro Asp Gly Tyr Thr Cys Cys Arg Leu Gln Ser Gly Ala Trp 
    290                 295                 300                 


Gly Cys Cys Pro Phe Thr Gln Ala Val Cys Cys Glu Asp His Ile His 
305                 310                 315                 320 


Cys Cys Pro Ala Gly Phe Thr Cys Asp Thr Gln Lys Gly Thr Cys Glu 
                325                 330                 335     


Gln Gly Pro His Gln Val Pro Trp Met Glu Lys Ala Pro Ala His Leu 
            340                 345                 350         


Ser Leu Pro Asp Pro Gln Ala Leu Lys Arg Asp Val Pro Cys Asp Asn 
        355                 360                 365             


Val Ser Ser Cys Pro Ser Ser Asp Thr Cys Cys Gln Leu Thr Ser Gly 
    370                 375                 380                 


Glu Trp Gly Cys Cys Pro Ile Pro Glu Ala Val Cys Cys Ser Asp His 
385                 390                 395                 400 


Gln His Cys Cys Pro Gln Gly Tyr Thr Cys Val Ala Glu Gly Gln Cys 
                405                 410                 415     


Gln Arg Gly Ser Glu Ile Val Ala Gly Leu Glu Lys Met Pro Ala Arg 
            420                 425                 430         


Arg Ala Ser Leu Ser His Pro Arg Asp Ile Gly Cys Asp Gln His Thr 
        435                 440                 445             


Ser Cys Pro Val Gly Gln Thr Cys Cys Pro Ser Leu Gly Gly Ser Trp 
    450                 455                 460                 


Ala Cys Cys Gln Leu Pro His Ala Val Cys Cys Glu Asp Arg Gln His 
465                 470                 475                 480 


Cys Cys Pro Ala Gly Tyr Thr Cys Asn Val Lys Ala Arg Ser Cys Glu 
                485                 490                 495     


Lys Glu Val Val Ser Ala Gln Pro Ala Thr Phe Leu Ala Arg Ser Pro 
            500                 505                 510         


His Val Gly Val Lys Asp Val Glu Cys Gly Glu Gly His Phe Cys His 
        515                 520                 525             


Asp Asn Gln Thr Cys Cys Arg Asp Asn Arg Gln Gly Trp Ala Cys Cys 
    530                 535                 540                 


Pro Tyr Arg Gln Gly Val Cys Cys Ala Asp Arg Arg His Cys Cys Pro 
545                 550                 555                 560 


Ala Gly Phe Arg Cys Ala Ala Arg Gly Thr Lys Cys Leu Arg Arg Glu 
                565                 570                 575     


Ala Pro Arg Trp Asp Ala Pro Leu Arg Asp Pro Ala Leu Arg Gln Leu 
            580                 585                 590         


Leu 
    


<210>  2
<211>  112
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc
<222>  (1)..(112)
<223>  N-terminal 1-112 amino acids of progranulin

<400>  2

Met Trp Thr Leu Val Ser Trp Val Ala Leu Thr Ala Gly Leu Val Ala 
1               5                   10                  15      


Gly Thr Arg Cys Pro Asp Gly Gln Phe Cys Pro Val Ala Cys Cys Leu 
            20                  25                  30          


Asp Pro Gly Gly Ala Ser Tyr Ser Cys Cys Arg Pro Leu Leu Asp Lys 
        35                  40                  45              


Trp Pro Thr Thr Leu Ser Arg His Leu Gly Gly Pro Cys Gln Val Asp 
    50                  55                  60                  


Ala His Cys Ser Ala Gly His Ser Cys Ile Phe Thr Val Ser Gly Thr 
65                  70                  75                  80  


Ser Ser Cys Cys Pro Phe Pro Glu Ala Val Ala Cys Gly Asp Gly His 
                85                  90                  95      


His Cys Cys Pro Arg Gly Phe His Cys Ser Ala Asp Gly Arg Ser Cys 
            100                 105                 110         


<210>  3
<211>  1782
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc
<222>  (1)..(1782)
<223>  Progranulin

<400>  3
atgtggaccc tggtgagctg ggtggcctta acagcagggc tggtggctgg aacgcggtgc       60

ccagatggtc agttctgccc tgtggcctgc tgcctggacc ccggaggagc cagctacagc      120

tgctgccgtc cccttctgga caaatggccc acaacactga gcaggcatct gggtggcccc      180

tgccaggttg atgcccactg ctctgccggc cactcctgca tctttaccgt ctcagggact      240

tccagttgct gccccttccc agaggccgtg gcatgcgggg atggccatca ctgctgccca      300

cggggcttcc actgcagtgc agacgggcga tcctgcttcc aaagatcagg taacaactcc      360

gtgggtgcca tccagtgccc tgatagtcag ttcgaatgcc cggacttctc cacgtgctgt      420

gttatggtcg atggctcctg ggggtgctgc cccatgcccc aggcttcctg ctgtgaagac      480

agggtgcact gctgtccgca cggtgccttc tgcgacctgg ttcacacccg ctgcatcaca      540

cccacgggca cccaccccct ggcaaagaag ctccctgccc agaggactaa cagggcagtg      600

gccttgtcca gctcggtcat gtgtccggac gcacggtccc ggtgccctga tggttctacc      660

tgctgtgagc tgcccagtgg gaagtatggc tgctgcccaa tgcccaacgc cacctgctgc      720

tccgatcacc tgcactgctg cccccaagac actgtgtgtg acctgatcca gagtaagtgc      780

ctctccaagg agaacgctac cacggacctc ctcactaagc tgcctgcgca cacagtgggg      840

gatgtgaaat gtgacatgga ggtgagctgc ccagatggct atacctgctg ccgtctacag      900

tcgggggcct ggggctgctg cccttttacc caggctgtgt gctgtgagga ccacatacac      960

tgctgtcccg cggggtttac gtgtgacacg cagaagggta cctgtgaaca ggggccccac     1020

caggtgccct ggatggagaa ggccccagct cacctcagcc tgccagaccc acaagccttg     1080

aagagagatg tcccctgtga taatgtcagc agctgtccct cctccgatac ctgctgccaa     1140

ctcacgtctg gggagtgggg ctgctgtcca atcccagagg ctgtctgctg ctcggaccac     1200

cagcactgct gcccccaggg ctacacgtgt gtagctgagg ggcagtgtca gcgaggaagc     1260

gagatcgtgg ctggactgga gaagatgcct gcccgccggg cttccttatc ccaccccaga     1320

gacatcggct gtgaccagca caccagctgc ccggtggggc agacctgctg cccgagcctg     1380

ggtgggagct gggcctgctg ccagttgccc catgctgtgt gctgcgagga tcgccagcac     1440

tgctgcccgg ctggctacac ctgcaacgtg aaggctcgat cctgcgagaa ggaagtggtc     1500

tctgcccagc ctgccacctt cctggcccgt agccctcacg tgggtgtgaa ggacgtggag     1560

tgtggggaag gacacttctg ccatgataac cagacctgct gccgagacaa ccgacagggc     1620

tgggcctgct gtccctaccg ccagggcgtc tgttgtgctg atcggcgcca ctgctgtcct     1680

gctggcttcc gctgcgcagc caggggtacc aagtgtttgc gcagggaggc cccgcgctgg     1740

gacgcccctt tgagggaccc agccttgaga cagctgctgt ga                        1782


<210>  4
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide derived from human progranulin gene

<400>  4
gatatcatgt ggaccctggt gagctgg                                           27


<210>  5
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  oligonucleotide derived from human progranulin gene

<400>  5
gatatccagc agctgtctca aggctgg                                           27


<210>  6
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide derived from human progranulin gene

<400>  6
gatatcatgg atagtcagtt cgaatgcccg                                        30


<210>  7
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide derived from human progranulin gene

<400>  7
gatatcatgc aggttgatgc ccactgc                                           27


<210>  8
<211>  48
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide derived from human progranulin gene and for 
       amplifying His-tag

<400>  8
gatatcatgc atcaccatca ccatcacatg tggaccctgg tgagctgg                    48


<210>  9
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide derived from human progranulin gene and for 
       amplifying His-tag

<400>  9
gatatcatgc atcaccatca ccatcacctg agcaggcatc tgggt                       45


<210>  10
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide derived from human progranulin gene and for 
       amplifying His-tag

<400>  10
gatatcatgc atcaccatca ccatcacaac aactccgtgg gtgcc                       45


<210>  11
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide derived from human progranulin gene and for 
       amplifying His-tag

<400>  11
gatatcatgc atcaccatca ccatcacgtg gccttgtcca gctcg                       45


<210>  12
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide derived from human progranulin gene and for 
       amplifying His-tag

<400>  12
gatatcgcac ttactctgga tcag                                              24


<210>  13
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide derived from human progranulin gene and for 
       amplifying His-tag

<400>  13
gatatcacag gtacccttct gcgt                                              24


<210>  14
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide derived from human progranulin gene and for 
       amplifying His-tag

<400>  14
gatatcctga cactgcccct cagc                                              24


<210>  15
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide derived from human progranulin gene

<400>  15
ccccggagga gccgcctaca gctgctgccg                                        30


<210>  16
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide derived from human progranulin gene

<400>  16
cggcagcagc tgtaggcggc tcctccgggg                                        30


<210>  17
<211>  29
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide derived from human progranulin gene

<400>  17
accgtctcag ggactgccag ttgctgccc                                         29


<210>  18
<211>  29
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide derived from human progranulin gene

<400>  18
gggcagcaac tggcagtccc tgagacggt                                         29


<210>  19
<211>  27
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc
<222>  (1)..(27)
<223>  Progranulin fragment

<400>  19

Ser Gly Gly Gly Gly Ser His Ser Cys Ile Phe Thr Val Ser Gly Thr 
1               5                   10                  15      


Ser Ser Cys Cys Pro Phe Pro Glu Ala Val Ala 
            20                  25          


<210>  20
<211>  27
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc
<222>  (1)..(27)
<223>  p-Progranulin fragment

<400>  20

Ser Gly Gly Gly Gly Ser His Ser Cys Ile Phe Thr Val Ser Gly Thr 
1               5                   10                  15      


Ser Ser Cys Cys Pro Phe Pro Glu Ala Val Ala 
            20                  25          


