                         SEQUENCE LISTING

<110>  SERVICIO ANDALUZ DE SALUD
       FIMABIS     
       UNIVERSIDAD DE MLAGA
 
<120>  Protena recombinante y usos en el diagnstico de la esclerosis 
       mltiple

<130>  IMABIS-80

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  2479
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  secuencia nucleotdica que codifica para IFNAR2.3

<400>  1
agatgtaaaa gtcaagagaa gactctaaaa atagcaaaga tgcttttgag ccagaatgcc       60

ttcatcttca gatcacttaa tttggttctc atggtgtata tcagcctcgt gtttggtatt      120

tcatatgatt cgcctgatta cacagatgaa tcttgcactt tcaagatatc attgcgaaat      180

ttccggtcca tcttatcatg ggaattaaaa aaccactcca ttgtaccaac tcactataca      240

ttgctgtata caatcatgag taaaccagaa gatttgaagg tggttaagaa ctgtgcaaat      300

accacaagat cattttgtga cctcacagat gagtggagaa gcacacacga ggcctatgtc      360

accgtcctag aaggattcag cgggaacaca acgttgttca gttgctcaca caatttctgg      420

ctggccatag acatgtcttt tgaaccacca gagtttgaga ttgttggttt taccaaccac      480

attaatgtga tggtgaaatt tccatctatt gttgaggaag aattacagtt tgatttatct      540

ctcgtcattg aagaacagtc agagggaatt gttaagaagc ataaacccga aataaaagga      600

aacatgagtg gaaatttcac ctatatcatt gacaagttaa ttccaaacac gaactactgt      660

gtatctgttt atttagagca cagtgatgag caagcagtaa taaagtctcc cttaaaatgc      720

accctccttc cacctggcca ggaatcagaa ttttcataac tttttagcct ggccatttcc      780

taacctgcca ccgttggaag ccatggatat ggtggaggtc atttacatca acagaaagaa      840

gaaagtgtgg gattataatt atgatgatga aagtgatagc gatactgagg cagcgcccag      900

gacaagtggc ggtggctata ccatgcatgg actgactgtc aggcctctgg gtcaggcctc      960

tgccacctct acagaatccc agttgataga cccggagtcc gaggaggagc ctgacctgcc     1020

tgaggttgat gtggagctcc ccacgatgcc aaaggacagc cctcagcagt tggaactctt     1080

gagtgggccc tgtgagagga gaaagagtcc actccaggac ccttttcccg aagaggacta     1140

cagctccacg gaggggtctg ggggcagaat taccttcaat gtggacttaa actctgtgtt     1200

tttgagagtt cttgatgacg aggacagtga cgacttagaa gcccctctga tgctatcgtc     1260

tcatctggaa gagatggttg acccagagga tcctgataat gtgcaatcaa accatttgct     1320

ggccagcggg gaagggacac agccaacctt tcccagcccc tcttcagagg gcctgtggtc     1380

cgaagatgct ccatctgatc aaagtgacac ttctgagtca gatgttgacc ttggggatgg     1440

ttatataatg agatgactcc aaaactattg aatgaacttg gacagacaag cacctacagg     1500

gttctttgtc tctgcatcct aacttgctgc cttatcgtct gcaagtgttc tccaagggaa     1560

ggaggaggaa actgtggtgt tcctttcttc caggtgacat cacctatgca cattcccagt     1620

atggggacca tagtatcatt cagtgcattg tttacatatt caaagtggtg cactttgaag     1680

gaagcacatg tgcacctttc ctttacacta atgcacttag gatgtttctg catcatgtct     1740

accagggagc agggttcccc acagtttcag aggtggtcca ggaccctatg atatttctct     1800

tctttcgttc tttttttttt ttttttgaga cagagtctcg ttctgtcgcc caagctggag     1860

cgcaatggtg tgatcttggc tcactgcaac atccgcctcc cgggttcagg tgattctcct     1920

gcctcagcct ccctcgcaag tagctgggat tacaggcgcc tgccaccatg cctagcaaat     1980

ttttgtattt ttagtggaga caggatttta ccatgttggc caggctggtc tcgaactcct     2040

gacctcaagt gatctgccct cctcagcctc gtaaagtgct gggattacag gggtgagccg     2100

ctgtgcctgg ctggccctgt gatatttctg tgaaataaat tgggccaggg tgggagcagg     2160

gaaagaaaag gaaaatagta gcaagagctg caaagcaggc aggaagggag gaggagagcc     2220

aggtgagcag tggagagaag gggggccctg cacaaggaaa cagggaagag ccatcgaagt     2280

ttcagtcggt gagccttggg cacctcaccc atgtcacatc ctgtctcctg caattggaat     2340

tccaccttgt ccagccctcc ccagttaaag tggggaagac agactttagg atcacgtgtg     2400

tgactaatac agaaaggaaa catggcgtcg gggagaggga taaaacctga atgccatatt     2460

ttaagttaaa aaaaaaaaa                                                  2479


<210>  2
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  secuencia aminoacdica IFNAR2.3

<400>  2

Met Leu Leu Ser Gln Asn Ala Phe Ile Phe Arg Ser Leu Asn Leu Val 
1               5                   10                  15      


Leu Met Val Tyr Ile Ser Leu Val Phe Gly Ile Ser Tyr Asp Ser Pro 
            20                  25                  30          


Asp Tyr Thr Asp Glu Ser Cys Thr Phe Lys Ile Ser Leu Arg Asn Phe 
        35                  40                  45              


Arg Ser Ile Leu Ser Trp Glu Leu Lys Asn His Ser Ile Val Pro Thr 
    50                  55                  60                  


His Tyr Thr Leu Leu Tyr Thr Ile Met Ser Lys Pro Glu Asp Leu Lys 
65                  70                  75                  80  


Val Val Lys Asn Cys Ala Asn Thr Thr Arg Ser Phe Cys Asp Leu Thr 
                85                  90                  95      


Asp Glu Trp Arg Ser Thr His Glu Ala Tyr Val Thr Val Leu Glu Gly 
            100                 105                 110         


Phe Ser Gly Asn Thr Thr Leu Phe Ser Cys Ser His Asn Phe Trp Leu 
        115                 120                 125             


Ala Ile Asp Met Ser Phe Glu Pro Pro Glu Phe Glu Ile Val Gly Phe 
    130                 135                 140                 


Thr Asn His Ile Asn Val Met Val Lys Phe Pro Ser Ile Val Glu Glu 
145                 150                 155                 160 


Glu Leu Gln Phe Asp Leu Ser Leu Val Ile Glu Glu Gln Ser Glu Gly 
                165                 170                 175     


Ile Val Lys Lys His Lys Pro Glu Ile Lys Gly Asn Met Ser Gly Asn 
            180                 185                 190         


Phe Thr Tyr Ile Ile Asp Lys Leu Ile Pro Asn Thr Asn Tyr Cys Val 
        195                 200                 205             


Ser Val Tyr Leu Glu His Ser Asp Glu Gln Ala Val Ile Lys Ser Pro 
    210                 215                 220                 


Leu Lys Cys Thr Leu Leu Pro Pro Gly Gln Glu Ser Glu Phe Ser 
225                 230                 235                 


<210>  3
<211>  331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Secuencia aminoacdica del anticuerpo de captura

<400>  3

Met Leu Leu Ser Gln Asn Ala Phe Ile Val Arg Ser Leu Asn Leu Val 
1               5                   10                  15      


Leu Met Val Tyr Ile Ser Leu Val Phe Gly Ile Ser Tyr Asp Ser Pro 
            20                  25                  30          


Asp Tyr Thr Asp Glu Ser Cys Thr Phe Lys Ile Ser Leu Arg Asn Phe 
        35                  40                  45              


Arg Ser Ile Leu Ser Trp Glu Leu Lys Asn His Ser Ile Val Pro Thr 
    50                  55                  60                  


His Tyr Thr Leu Leu Tyr Thr Ile Met Ser Lys Pro Glu Asp Leu Lys 
65                  70                  75                  80  


Val Val Lys Asn Cys Ala Asn Thr Thr Arg Ser Phe Cys Asp Leu Thr 
                85                  90                  95      


Asp Glu Trp Arg Ser Thr His Glu Ala Tyr Val Thr Val Leu Glu Gly 
            100                 105                 110         


Phe Ser Gly Asn Thr Thr Leu Phe Ser Cys Ser His Asn Phe Trp Leu 
        115                 120                 125             


Ala Ile Asp Met Ser Phe Glu Pro Pro Glu Phe Glu Ile Val Gly Phe 
    130                 135                 140                 


Thr Asn His Ile Asn Val Met Val Lys Phe Pro Ser Ile Val Glu Glu 
145                 150                 155                 160 


Glu Leu Gln Phe Asp Leu Ser Leu Val Ile Glu Glu Gln Ser Glu Gly 
                165                 170                 175     


Ile Val Lys Lys His Lys Pro Glu Ile Lys Gly Asn Met Ser Gly Asn 
            180                 185                 190         


Phe Thr Tyr Ile Ile Asp Lys Leu Ile Pro Asn Thr Asn Tyr Cys Val 
        195                 200                 205             


Ser Val Tyr Leu Glu His Ser Asp Glu Gln Ala Val Ile Lys Ser Pro 
    210                 215                 220                 


Leu Lys Cys Thr Leu Leu Pro Pro Gly Gln Glu Ser Glu Ser Ala Glu 
225                 230                 235                 240 


Ser Ala Lys Ile Gly Gly Ile Ile Thr Val Phe Leu Ile Ala Leu Val 
                245                 250                 255     


Leu Thr Ser Thr Ile Val Thr Leu Lys Trp Ile Gly Tyr Ile Cys Leu 
            260                 265                 270         


Arg Asn Ser Leu Pro Lys Val Leu Arg Gln Gly Leu Thr Lys Gly Trp 
        275                 280                 285             


Asn Ala Val Ala Ile His Arg Cys Ser His Asn Ala Leu Gln Ser Glu 
    290                 295                 300                 


Thr Pro Glu Leu Lys Gln Ser Ser Cys Leu Ser Phe Pro Ser Ser Trp 
305                 310                 315                 320 


Asp Tyr Lys Arg Ala Ser Leu Cys Pro Ser Asp 
                325                 330     


<210>  4
<211>  331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  secuencia aminoacdica del anticuerpo de deteccin

<400>  4

Met Leu Leu Ser Gln Asn Ala Phe Ile Val Arg Ser Leu Asn Leu Val 
1               5                   10                  15      


Leu Met Val Tyr Ile Ser Leu Val Phe Gly Ile Ser Tyr Asp Ser Pro 
            20                  25                  30          


Asp Tyr Thr Asp Glu Ser Cys Thr Phe Lys Ile Ser Leu Arg Asn Phe 
        35                  40                  45              


Arg Ser Ile Leu Ser Trp Glu Leu Lys Asn His Ser Ile Val Pro Thr 
    50                  55                  60                  


His Tyr Thr Leu Leu Tyr Thr Ile Met Ser Lys Pro Glu Asp Leu Lys 
65                  70                  75                  80  


Val Val Lys Asn Cys Ala Asn Thr Thr Arg Ser Phe Cys Asp Leu Thr 
                85                  90                  95      


Asp Glu Trp Arg Ser Thr His Glu Ala Tyr Val Thr Val Leu Glu Gly 
            100                 105                 110         


Phe Ser Gly Asn Thr Thr Leu Phe Ser Cys Ser His Asn Phe Trp Leu 
        115                 120                 125             


Ala Ile Asp Met Ser Phe Glu Pro Pro Glu Phe Glu Ile Val Gly Phe 
    130                 135                 140                 


Thr Asn His Ile Asn Val Met Val Lys Phe Pro Ser Ile Val Glu Glu 
145                 150                 155                 160 


Glu Leu Gln Phe Asp Leu Ser Leu Val Ile Glu Glu Gln Ser Glu Gly 
                165                 170                 175     


Ile Val Lys Lys His Lys Pro Glu Ile Lys Gly Asn Met Ser Gly Asn 
            180                 185                 190         


Phe Thr Tyr Ile Ile Asp Lys Leu Ile Pro Asn Thr Asn Tyr Cys Val 
        195                 200                 205             


Ser Val Tyr Leu Glu His Ser Asp Glu Gln Ala Val Ile Lys Ser Pro 
    210                 215                 220                 


Leu Lys Cys Thr Leu Leu Pro Pro Gly Gln Glu Ser Glu Ser Ala Glu 
225                 230                 235                 240 


Ser Ala Lys Ile Gly Gly Ile Ile Thr Val Phe Leu Ile Ala Leu Val 
                245                 250                 255     


Leu Thr Ser Thr Ile Val Thr Leu Lys Trp Ile Gly Tyr Ile Cys Leu 
            260                 265                 270         


Arg Asn Ser Leu Pro Lys Val Leu Arg Gln Gly Leu Thr Lys Gly Trp 
        275                 280                 285             


Asn Ala Val Ala Ile His Arg Cys Ser His Asn Ala Leu Gln Ser Glu 
    290                 295                 300                 


Thr Pro Glu Leu Lys Gln Ser Ser Cys Leu Ser Phe Pro Ser Ser Trp 
305                 310                 315                 320 


Asp Tyr Lys Arg Ala Ser Leu Cys Pro Ser Asp 
                325                 330     


<210>  5
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador sentido

<400>  5
taaggcctct gtcgacattt catatgattc gcctgattac acgatg                      46


<210>  6
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cebador secuencia antisentido

<400>  6
cagaattcgc aagctttgaa aattctgatt cctggccagg tggaa                       45


