               SEQUENCE LISTING

<110> CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

<120> In vitro production of DNA minicircles comprising less than 250 base pairs

<130> Y719EP

<140> EP12306454.5
<141> 2012-11-22

<160> 21

<170> BiSSAP 1.2

<210> 1
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<400> 1
ccaaggcctc aagactgatc tacagatcaa ctccttgcca ttgccgtacg agatctagcc     60

tctagctagt cgtgctgatc atactacgag catgg                                95


<210> 2
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<400> 2
gcctcaacca aggactgatc tacagatcaa ctccttgcca ttgccgtacg agatctagcc     60

tctagctagt cgtgctgatc atactacgag catgg                                95


<210> 3
<211> 84
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..84
<223> /mol_type="unassigned DNA"
      /note="linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(84)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 84 of complementary
      strand"

<400> 3
ccaaggcctc aagactgatc tacagatcaa ctccttgcca ttgccgtacg agatctagcc     60

tctagctagt cgtgctgatc atgg                                            84


<210> 4
<211> 116
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..116
<223> /mol_type="unassigned DNA"
      /note="linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> complement(21)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 21 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(21^22)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 21 and 22 of complementary strand"

<220> 
<221> misc_feature
<222> 55^56
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56"

<220> 
<221> misc_feature
<222> 56
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 56"

<220> 
<221> misc_feature
<222> complement(70)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 70 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(70^71)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 70 and 71 of complementary strand"

<220> 
<221> misc_feature
<222> 95^96
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 95 and 96"

<220> 
<221> misc_feature
<222> 96
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 96"

<220> 
<221> misc_feature
<222> complement(116)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 116 of complementary
      strand"

<400> 4
ccaaggcctc aagactgatc tacagatcaa ctccttgcca ttgccgtacg agatctagcc     60

tctagctagt cgtgctgatc atactacgag catggcgagt gagcttgacg cgatgg        116


<210> 5
<211> 158
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..158
<223> /mol_type="unassigned DNA"
      /note="linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> 55^56
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 and 56 of
      complementary strand"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> 96
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 96"

<220> 
<221> misc_feature
<222> 95^96
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 95 and 96"

<220> 
<221> misc_feature
<222> complement(110^111)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 110 and 111 of complementary strand"

<220> 
<221> misc_feature
<222> complement(158)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 158 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(110)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 110 of complementary
      strand"

<400> 5
ccaaggcctc aagactgatc tacagatcaa ctccttgcca ttgccgtacg agatctagcc     60

tctagctagt cgtgctgatc atactacgag catggcgagt gagcttgacg cgatgacttg    120

gaccaagaca ggaagcacat gttcctggag attactgg                            158


<210> 6
<211> 199
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..199
<223> /mol_type="unassigned DNA"
      /note="Linear nicked blunt-ended suplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> 96
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 96"

<220> 
<221> misc_feature
<222> 159
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 159"

<220> 
<221> misc_feature
<222> complement(173)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 173 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(110)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 110 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(199)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 199 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> 95^96
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 95 and 96"

<220> 
<221> misc_feature
<222> complement(110^111)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 110 and 111 of complementary strand"

<220> 
<221> misc_feature
<222> 158^159
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 158 and 159"

<220> 
<221> misc_feature
<222> complement(173^174)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 173 and 174 of complementary strand"

<400> 6
ccaaggcctc aagactgatc tacagatcaa ctccttgcca ttgccgtacg agatctagcc     60

tctagctagt cgtgctgatc atactacgag catggcgagt gagcttgacg cgatgacttg    120

gaccaagaca ggaagcacat gttcctggag attactggcc tagcacgtga cggctgcagg    180

tatctaagag agcgagtgg                                                 199


<210> 7
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 29..38
<223> /note="Consensus sequence for NF kappa B transcription factor
      from nucleotide 29 to nucleotide 38"

<400> 7
ccaaggcctc aagactgatc tacagatcgg gactttccca ttgccgtacg agatctagcc     60

tctagctagt cgtgctgatc atactacgag catgg                                95


<210> 8
<211> 96
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..96
<223> /mol_type="unassigned DNA"
      /note="linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> 29..38
<223> /note="Consensus sequence for NF kappa B transcription factor
      from nucleotide 29 to nucleotide 38"

<220> 
<221> misc_feature
<222> 66..75
<223> /note="Consensus sequence for NF kappa B transcription factor
      from nucleotide 66 to nucleotide 75"

<400> 8
ccaaggcctc aagactgatc tacagatcgg gactttccca ttgccgtacg agatctagcc     60

tctaggggac tttccctgat catactacga gcatgg                               96


<210> 9
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> 30..39
<223> /note="Consensus sequence for NF kappa B transcription factor
      from nucleotide 30 to nucleotide 39"

<220> 
<221> misc_feature
<222> 45..54
<223> /note="Consensus sequence for NF kappa B transcription factor
      from nucleotide 45 to nucleotide 54"

<220> 
<221> misc_feature
<222> 60..69
<223> /note="Consensus sequence for NF kappa B transcription factor
      from nucleotide 60 to nucleotide 69"

<400> 9
ccttcggatt atcggacggc gctagcctgg ggactttcca gctggggact ttccagctgg     60

ggactttcca ggagattcag gatcaggtga cttgg                                95


<210> 10
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Linear nicked blunt-ended sequence"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> 8..17
<223> /note="Consensus sequence for NF kappa B transcription factor
      from nucleotide 8 to nucleotide 17"

<220> 
<221> misc_feature
<222> 21..30
<223> /note="Consensus sequence for NF kappa B transcription factor
      from nucleotide 21 to nucleotide 30"

<220> 
<221> misc_feature
<222> 34..43
<223> /note="Consensus sequence for NF kappa B transcription factor
      from nucleotide 34 to nucleotide 43"

<220> 
<221> misc_feature
<222> 54..64
<223> /note="Consensus sequence for NF kappa B transcription factor
      from nucleotide 54 to nucleotide 64"

<220> 
<221> misc_feature
<222> 68..77
<223> /note="Consensus sequence for NF kappa B transcription factor
      from nucleotide 68 to nucleotide 77"

<220> 
<221> misc_feature
<222> 81..90
<223> /note="Consensus sequence for NF kappa B transcription factor
      from nucleotide 81 to nucleotide 90"

<400> 10
ccttatgggg actttccatg gggactttcc atggggactt tccatagctt gctggggact     60

ttccatgggg actttccatg gggactttcc attgg                                95


<210> 11
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Liner nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> 19..28
<223> /note="Consensus sequence for NF kappa B transcription factor
      from nucleotide 19 to nucleotide 28"

<220> 
<221> misc_feature
<222> 49..57
<223> /note="Consensus sequence for STAT3 transcription factor from
      nucleotide 49 to nucleotide 57"

<220> 
<221> misc_feature
<222> 73..84
<223> /note="Consensus sequence for ETS1 transcription factor from
      nucleotide 73 to nucleotide 84"

<400> 11
ccttcggatt atcggacggg gactttccgt actcgttgct acgtgcattt cccgtaaatc     60

tccatggatc caggaagcac ttcctggtga cttgg                                95


<210> 12
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Linear nicked blunt-ended duplec"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> 7..90
<223> /note="human telomeric sequence from nucleotide 7 to nucleotide
      90"

<400> 12
ccttggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg     60

ttagggttag ggttagggtt agggttaggg tttgg                                95


<210> 13
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> 55^56
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> complement(7)
<223> /note="mismatch, nucleotide 7 of complementary strand is g"

<220> 
<221> misc_feature
<222> complement(23)
<223> /note="mismatch, nucleotide 23 of complementary strand is g"

<220> 
<221> misc_feature
<222> complement(71)
<223> /note="mismatch, nucleotide 71 of complementary strand is g"

<400> 13
ccaaggtctc aagactgatc tatagatcaa ctccttgcca ttgccgtacg agatctagcc     60

tctagctagt tgtgctgatc atactacgag catgg                                95


<210> 14
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> complement(41)
<223> /note="nucleotide 71 on complementary strand is cyanine 3 dt"

<220> 
<221> misc_feature
<222> complement(71)
<223> /note="nucleotide 71 on complementary strand is 8-oxoguanine"

<400> 14
ccaaggcctc aagactgatc tacagatcaa ctccttgcca ttgccgtacg agatctagcc     60

tctagctagt cgtgctgatc atactacgag catgg                                95


<210> 15
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> complement(20)
<223> /note="nucleotide 20 on complementary strand is 8 oxoguanine"

<220> 
<221> misc_feature
<222> complement(49)
<223> /note="nucleotide 49 on complementary strand is 8 oxoguanine"

<400> 15
ccaaggcctc aagactgatc tacagatcaa ctccttgcca ttgccgtacg agatctagcc     60

tctagctagt cgtgctgatc atactacgag catgg                                95


<210> 16
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> complement(85)
<223> /note="nucleotide 85 on complementary strand is biotin dt"

<400> 16
ccttcggatt atcggacggc gctagcctgg ggactttcca gctggggact ttccagctgg     60

ggactttcca ggagattcag gatcaggtga cttgg                                95


<210> 17
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 23
<223> /note="nucleotide 23 is biotine dt"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(85)
<223> /note="nucleotide 85 on complementary strand is biotine dt"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<400> 17
ccttcggatt atcggacggc gcnagcctgg ggactttcca gctggggact ttccagctgg     60

ggactttcca ggagattcag gatcaggtga cttgg                                95


<210> 18
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> complement(85)
<223> /note="nucleotide 85 on complementary strand is cyanin 3 dt"

<400> 18
ccttcggatt atcggacggc gctagcctgg ggactttcca gctggggact ttccagctgg     60

ggactttcca ggagattcag gatcaggtga cttgg                                95


<210> 19
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Linear nickd blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 23
<223> /note="nucleotide 23 is cyanine3 dt"

<220> 
<221> misc_feature
<222> complement(85)
<223> /note="nucleotide 85 on complementary strand is cyanine 3 dt"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<400> 19
ccttcggatt atcggacggc gcnagcctgg ggactttcca gctggggact ttccagctgg     60

ggactttcca ggagattcag gatcaggtga cttgg                                95


<210> 20
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Linear nicked blunt-ended duplex"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 of complementary strand"

<220> 
<221> misc_feature
<222> complement(85)
<223> /note="nucleotide 85 on complementary strand is
      carboxyfluoroscein dt"

<400> 20
ccttcggatt atcggacggc gctagcctgg ggactttcca gctggggact ttccagctgg     60

ggactttcca ggagattcag gatcaggtga cttgg                                95


<210> 21
<211> 95
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..95
<223> /mol_type="unassigned DNA"
      /note="Linear nicked blunt-ended sequence"
      /organism="Artificial Sequence"

<220> 
<221> misc_feature
<222> 23
<223> /note="nucleotide 23 is carboxyfluoroscein dt"

<220> 
<221> misc_feature
<222> complement(85)
<223> /note="nucleotide 85 on complementary strand is
      carboxyfluoroscein dt"

<220> 
<221> misc_feature
<222> 1
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 1"

<220> 
<221> misc_feature
<222> 41
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 41"

<220> 
<221> misc_feature
<222> complement(55)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 55 of complementary
      strand"

<220> 
<221> misc_feature
<222> complement(95)
<223> /note="A phosphate group is attached to the 5' carbon in the
      sugar-ring of the deoxyribose of nucleotide 95 of complementary
      strand"

<220> 
<221> misc_feature
<222> 40^41
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 40 and 41"

<220> 
<221> misc_feature
<222> complement(55^56)
<223> /note="Presence of a nick (no phosphodiester bond) between
      adjacent nucleotides 55 and 56 on complementary strand"

<400> 21
ccttcggatt atcggacggc gcnagcctgg ggactttcca gctggggact ttccagctgg     60

ggactttcca ggagattcag gatcaggtga cttgg                                95


