                         SEQUENCE LISTING

<110>  toxogen GmbH
 
<120>  Methods for the manufacture of proteolytically processed 
       polypeptides

<130>  757-1

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  333
<212>  PRT
<213>  Clostridium Botulinum

<400>  1

Val Gln Gly Gln Ser Val Lys Gly Val Gly Lys Thr Ser Leu Asp Gly 
1               5                   10                  15      


Leu Val Asn Ile Asp Val Thr Tyr Gly Asn Gly Lys Tyr Tyr Leu Lys 
            20                  25                  30          


Asp Ser Asn Lys Asn Ile Tyr Leu Tyr Asp Leu Lys Asn Gln Val Asp 
        35                  40                  45              


Glu Tyr Asp Leu Tyr Asn Tyr Leu Ser Arg Pro Asn Tyr Lys Gln Ile 
    50                  55                  60                  


Leu Met Ser Lys Ser Glu Leu Ile Ser Asn Tyr Asn Asn Asn Phe Ile 
65                  70                  75                  80  


Ala Asn Asn Gln Val Asn Ser Val Asp Ala Tyr Val Asn Thr Asn Lys 
                85                  90                  95      


Thr Tyr Asp Tyr Tyr Lys Asn Lys Leu Asn Arg Asn Ser Ile Asp Asn 
            100                 105                 110         


Lys Gly Met Asn Ile Asn Gly Phe Val His Val Gly Arg Asn Tyr Gly 
        115                 120                 125             


Asn Ala Phe Trp Tyr Gly Pro Tyr Asp Gly Met Phe Phe Gly Asp Gly 
    130                 135                 140                 


Asp Gly Ile Tyr Phe Ser Ser Leu Ala Lys Ser Leu Asp Val Val Gly 
145                 150                 155                 160 


His Glu Leu Ser His Gly Val Thr Asn Lys Glu Ser Asn Leu Lys Tyr 
                165                 170                 175     


Glu Asn Glu Ser Gly Ala Leu Asn Glu Ser Phe Ser Asp Ile Met Gly 
            180                 185                 190         


Val Ala Val Glu Gly Lys Asn Phe Val Leu Gly Glu Asp Cys Trp Val 
        195                 200                 205             


Ala Gly Gly Val Met Arg Asp Met Glu Asn Pro Ser Arg Gly Gly Gln 
    210                 215                 220                 


Pro Ala His Met Lys Asp Tyr Lys Tyr Lys Thr Met Asn Asp Asp Asn 
225                 230                 235                 240 


Gly Gly Val His Thr Asn Ser Gly Ile Ile Asn His Ala Ala Tyr Leu 
                245                 250                 255     


Val Ala Asp Gly Ile Glu Lys Thr Gly Ala Lys Asn Ser Lys Asp Ile 
            260                 265                 270         


Met Gly Lys Ile Phe Tyr Thr Ala Asn Cys Tyr Lys Trp Asp Glu Thr 
        275                 280                 285             


Thr Asn Phe Ala Lys Cys Arg Asn Asp Val Val Gln Val Thr Lys Glu 
    290                 295                 300                 


Leu Tyr Gly Glu Asn Ser Asn Tyr Val Lys Ile Val Glu Lys Ala Phe 
305                 310                 315                 320 


Asp Gln Val Gly Ile Thr Ala Thr Pro Gln Leu Pro Leu 
                325                 330             


<210>  2
<211>  581
<212>  PRT
<213>  Clostridium Botulinum

<400>  2

Met Lys Ser Lys Lys Leu Leu Ala Thr Val Leu Ser Ala Val Ile Thr 
1               5                   10                  15      


Phe Ser Thr Val Ser Ala Val Tyr Ala Ala Pro Val Gly Lys Glu Ser 
            20                  25                  30          


Lys Val Glu Pro Lys Thr Thr Thr Ile Thr Trp Glu Lys Asn Glu Gln 
        35                  40                  45              


Asn Thr Lys Lys Ala Ala Thr Asp Ile Thr Glu Lys Lys Phe Asn Asn 
    50                  55                  60                  


Ser Glu Glu Ile Thr Lys Phe Phe Glu Lys Asn Ile Ser Lys Phe Gly 
65                  70                  75                  80  


Val Gln Lys Gly Ser Leu Lys Asn Thr Lys Thr Val Lys Asp Glu Lys 
                85                  90                  95      


Gly Lys Thr Asn Tyr His Met Ile Tyr Glu Val Glu Gly Ile Pro Val 
            100                 105                 110         


Tyr Tyr Gly Arg Ile Val Phe Thr Thr Glu Lys Asp Ser Ser Met Asp 
        115                 120                 125             


Ser Ile Asn Gly Arg Ile Asp Thr Val Phe Glu Asn Gly Asn Trp Lys 
    130                 135                 140                 


Asn Lys Ile Lys Leu Ser Lys Glu Asp Ala Ile Ala Lys Ala Lys Asn 
145                 150                 155                 160 


Asp Ile Lys Asp Glu Lys Ala Thr Ser Lys Lys Thr Asp Leu Tyr Leu 
                165                 170                 175     


Tyr Asn Phe Glu Gly Lys Pro Tyr Val Val Tyr Leu Val Asp Leu Ile 
            180                 185                 190         


Thr Asp Asn Gly Ser Trp Thr Val Phe Val Asn Ala Glu Asp Gly Ser 
        195                 200                 205             


Ile Val Asn Lys Phe Asn Asn Thr Pro Thr Leu Ile Asp Thr Lys Asp 
    210                 215                 220                 


Gln Lys Leu Pro Asn Ala Lys Lys Ile Lys Asp Glu Ala Lys Lys Ala 
225                 230                 235                 240 


Ser Asn Ala Asn Asn Val Ile Asp Val Gln Gly Gln Ser Val Lys Gly 
                245                 250                 255     


Val Gly Lys Thr Ser Leu Asp Gly Leu Val Asn Ile Asp Val Thr Tyr 
            260                 265                 270         


Gly Asn Gly Lys Tyr Tyr Leu Lys Asp Ser Asn Lys Asn Ile Tyr Leu 
        275                 280                 285             


Tyr Asp Leu Lys Asn Gln Val Asp Glu Tyr Asp Leu Tyr Asn Tyr Leu 
    290                 295                 300                 


Ser Arg Pro Asn Tyr Lys Gln Ile Leu Met Ser Lys Ser Glu Leu Ile 
305                 310                 315                 320 


Ser Asn Tyr Asn Asn Asn Phe Ile Ala Asn Asn Gln Val Asn Ser Val 
                325                 330                 335     


Asp Ala Tyr Val Asn Thr Asn Lys Thr Tyr Asp Tyr Tyr Lys Asn Lys 
            340                 345                 350         


Leu Asn Arg Asn Ser Ile Asp Asn Lys Gly Met Asn Ile Asn Gly Phe 
        355                 360                 365             


Val His Val Gly Arg Asn Tyr Gly Asn Ala Phe Trp Tyr Gly Pro Tyr 
    370                 375                 380                 


Asp Gly Met Phe Phe Gly Asp Gly Asp Gly Ile Tyr Phe Ser Ser Leu 
385                 390                 395                 400 


Ala Lys Ser Leu Asp Val Val Gly His Glu Leu Ser His Gly Val Thr 
                405                 410                 415     


Asn Lys Glu Ser Asn Leu Lys Tyr Glu Asn Glu Ser Gly Ala Leu Asn 
            420                 425                 430         


Glu Ser Phe Ser Asp Ile Met Gly Val Ala Val Glu Gly Lys Asn Phe 
        435                 440                 445             


Val Leu Gly Glu Asp Cys Trp Val Ala Gly Gly Val Met Arg Asp Met 
    450                 455                 460                 


Glu Asn Pro Ser Arg Gly Gly Gln Pro Ala His Met Lys Asp Tyr Lys 
465                 470                 475                 480 


Tyr Lys Thr Met Asn Asp Asp Asn Gly Gly Val His Thr Asn Ser Gly 
                485                 490                 495     


Ile Ile Asn His Ala Ala Tyr Leu Val Ala Asp Gly Ile Glu Lys Thr 
            500                 505                 510         


Gly Ala Lys Asn Ser Lys Asp Ile Met Gly Lys Ile Phe Tyr Thr Ala 
        515                 520                 525             


Asn Cys Tyr Lys Trp Asp Glu Thr Thr Asn Phe Ala Lys Cys Arg Asn 
    530                 535                 540                 


Asp Val Val Gln Val Thr Lys Glu Leu Tyr Gly Glu Asn Ser Asn Tyr 
545                 550                 555                 560 


Val Lys Ile Val Glu Lys Ala Phe Asp Gln Val Gly Ile Thr Ala Thr 
                565                 570                 575     


Pro Gln Leu Pro Leu 
            580     


<210>  3
<211>  1296
<212>  PRT
<213>  Clostridium Botulinum

<400>  3

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Asn Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Gln Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  4
<211>  25
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/A1
<222>  (1)..(25)

<400>  4

Cys Val Arg Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly 
1               5                   10                  15      


Tyr Asn Lys Ala Leu Asn Asp Leu Cys 
            20                  25  


<210>  5
<211>  25
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/A2/A6
<222>  (1)..(25)

<400>  5

Cys Val Arg Gly Ile Ile Pro Phe Lys Thr Lys Ser Leu Asp Glu Gly 
1               5                   10                  15      


Tyr Asn Lys Ala Leu Asn Asp Leu Cys 
            20                  25  


<210>  6
<211>  25
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/A3
<222>  (1)..(25)

<400>  6

Cys Val Arg Gly Ile Ile Pro Phe Lys Thr Lys Ser Leu Asp Glu Gly 
1               5                   10                  15      


Tyr Asn Lys Ala Leu Asn Asp Leu Cys 
            20                  25  


<210>  7
<211>  25
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/A3
<222>  (1)..(25)

<400>  7

Cys Val Arg Gly Ile Ile Pro Phe Lys Thr Lys Ser Leu Asp Glu Gly 
1               5                   10                  15      


Tyr Asn Lys Ala Leu Asn Tyr Leu Cys 
            20                  25  


<210>  8
<211>  25
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/A4
<222>  (1)..(25)

<400>  8

Cys Val Arg Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Glu Gly 
1               5                   10                  15      


Tyr Asn Lys Ala Leu Asn Glu Leu Cys 
            20                  25  


<210>  9
<211>  25
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/A5
<222>  (1)..(25)

<400>  9

Cys Val Arg Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Glu Gly 
1               5                   10                  15      


Tyr Asn Lys Ala Leu Asn Asp Leu Cys 
            20                  25  


<210>  10
<211>  25
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/A7
<222>  (1)..(25)

<400>  10

Trp Val Arg Gly Ile Ile Pro Phe Lys Pro Lys Ser Leu Asp Glu Gly 
1               5                   10                  15      


Ser Asn Lys Ala Leu Asn Asp Leu Cys 
            20                  25  


<210>  11
<211>  10
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/B1/B4bv/B6
<222>  (1)..(10)

<400>  11

Cys Lys Ser Val Lys Ala Pro Gly Ile Cys 
1               5                   10  


<210>  12
<211>  10
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/B2/B3
<222>  (1)..(10)

<400>  12

Cys Lys Ser Val Arg Ala Pro Gly Ile Cys 
1               5                   10  


<210>  13
<211>  10
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/B5np
<222>  (1)..(10)

<400>  13

Cys Lys Ser Val Lys Val Pro Gly Ile Cys 
1               5                   10  


<210>  14
<211>  17
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/C/CD
<222>  (1)..(17)

<400>  14

Cys His Lys Ala Ile Asp Gly Arg Ser Leu Tyr Asn Lys Thr Leu Asp 
1               5                   10                  15      


Cys 
    


<210>  15
<211>  14
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/D
<222>  (1)..(14)

<400>  15

Cys Leu Arg Leu Thr Lys Asn Ser Arg Asp Asp Ser Thr Cys 
1               5                   10                  


<210>  16
<211>  14
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/DC
<222>  (1)..(14)

<400>  16

Cys Leu Arg Leu Thr Arg Asn Ser Arg Asp Asp Ser Thr Cys 
1               5                   10                  


<210>  17
<211>  15
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/E1-E5
<222>  (1)..(15)

<400>  17

Cys Lys Asn Ile Val Ser Val Lys Gly Ile Arg Lys Ser Ile Cys 
1               5                   10                  15  


<210>  18
<211>  15
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/E6
<222>  (1)..(15)

<400>  18

Cys Lys Asn Ile Val Phe Ser Lys Gly Ile Arg Lys Ser Ile Cys 
1               5                   10                  15  


<210>  19
<211>  17
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/F1/F6
<222>  (1)..(17)

<400>  19

Cys Lys Ser Val Ile Pro Arg Lys Gly Thr Lys Ala Pro Pro Arg Leu 
1               5                   10                  15      


Cys 
    


<210>  20
<211>  17
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/F2/F3
<222>  (1)..(17)

<400>  20

Cys Lys Ser Ile Ile Pro Arg Lys Gly Thr Lys Gln Ser Pro Ser Leu 
1               5                   10                  15      


Cys 
    


<210>  21
<211>  17
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/F4
<222>  (1)..(17)

<400>  21

Cys Lys Ser Ile Ile Pro Arg Lys Gly Thr Lys Ala Pro Pro Arg Leu 
1               5                   10                  15      


Cys 
    


<210>  22
<211>  15
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/F5
<222>  (1)..(15)

<400>  22

Cys Leu Asn Ser Ser Phe Lys Lys Asn Thr Lys Lys Pro Leu Cys 
1               5                   10                  15  


<210>  23
<211>  15
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/F7
<222>  (1)..(15)

<400>  23

Cys Lys Ser Ile Val Ser Lys Lys Gly Thr Lys Asn Ser Leu Cys 
1               5                   10                  15  


<210>  24
<211>  15
<212>  PRT
<213>  Clostridium Botulinum


<220>
<221>  Loop BoNT/G
<222>  (1)..(15)

<400>  24

Cys Lys Pro Val Met Tyr Lys Asn Thr Gly Lys Ser Glu Gln Cys 
1               5                   10                  15  


<210>  25
<211>  29
<212>  PRT
<213>  Clostridium Tetani


<220>
<221>  Loop TeNT
<222>  (1)..(29)

<400>  25

Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile Arg Glu Asn Leu Tyr Asn 
1               5                   10                  15      


Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly Glu Leu Cys 
            20                  25                  


<210>  26
<211>  1005
<212>  DNA
<213>  C. Botulinum

<400>  26
atggttcaag gtcaaagcgt taaaggagta ggaaaaacta gcttggatgg actagtaaat       60

attgatgtaa cttatggaaa tggaaaatac tatttaaaag atagcaacaa aaatatttat      120

ctatatgact taaaaaatca agttgatgaa tatgatctat acaattatct tagtagacct      180

aactataaac aaatattaat gagcaaatct gaattaatat ctaattacaa taataatttt      240

atagccaaca atcaggttaa ttctgtagat gcttatgtaa acacaaataa aacctatgat      300

tattataaaa acaaattaaa tagaaacagt attgataata agggtatgaa tattaatggg      360

tttgttcatg taggtagaaa ttatggtaat gctttttggt acggtccata tgatgggatg      420

ttctttggcg atggcgacgg aatatacttc tcttcccttg caaaatcttt agatgttgta      480

ggccacgaat taagtcatgg tgtaacaaat aaagagtcta atcttaaata tgaaaatgaa      540

tctggtgccc taaatgaatc tttctcagat attatgggag tagctgttga gggtaaaaac      600

tttgtactag gtgaagattg ctgggttgct ggaggagtaa tgagagatat ggaaaatcca      660

tccagaggag gccaaccagc tcatatgaaa gattataaat acaaaactat gaatgacgat      720

aacggtggtg ttcatacaaa ttcaggtata ataaaccatg ctgcttattt agttgcagat      780

ggaatagaaa aaactggtgc aaaaaatagt aaagatatta tgggaaaaat attctataca      840

gctaattgct ataaatggga tgaaacaaca aattttgcta agtgcagaaa tgatgtagtc      900

caagttacta aagaacttta tggcgaaaat agcaactatg taaaaattgt tgaaaaagct      960

tttgaccaag ttggaataac tgctacacct caattaccat tataa                     1005


<210>  27
<211>  1746
<212>  DNA
<213>  Clostridium Botulinum

<400>  27
atgaaaagta aaaaattatt agctacagtg ctaagtgccg tgatcacttt ttctactgtt       60

tctgcagttt atgctgcgcc tgtaggaaaa gaaagtaaag ttgaaccaaa aactacaaca      120

ataacttggg aaaaaaatga acaaaatact aaaaaagctg ctactgatat aactgaaaag      180

aaatttaaca attctgagga gataactaaa ttctttgaaa aaaatatatc taaatttggt      240

gtacaaaaag gttctcttaa aaacaccaag actgtaaaag acgaaaaagg taaaactaac      300

tatcatatga tttatgaagt agaaggtata cctgtatact atggaagaat tgtttttaca      360

actgaaaaag actcctccat ggattctata aacggtagaa ttgatactgt ttttgaaaat      420

gggaattgga aaaacaaaat caaactatca aaagaagatg ctatagcaaa agctaaaaat      480

gatattaaag atgaaaaagc aactagtaaa aagaccgatt tatatctgta taattttgag      540

ggcaaacctt atgtagttta tttagtagat ctaattacag acaacgggag ttggacggtt      600

ttcgttaatg ctgaggatgg ttctatagta aataaattta ataatactcc tactttaatt      660

gatactaaag atcaaaaatt acccaatgct aaaaaaatta aagatgaagc taaaaaagct      720

agtaatgcaa ataatgtaat tgatgttcaa ggtcaaagcg ttaaaggagt aggaaaaact      780

agcttggatg gactagtaaa tattgatgta acttatggaa atggaaaata ctatttaaaa      840

gatagcaaca aaaatattta tctatatgac ttaaaaaatc aagttgatga atatgatcta      900

tacaattatc ttagtagacc taactataaa caaatattaa tgagcaaatc tgaattaata      960

tctaattaca ataataattt tatagccaac aatcaggtta attctgtaga tgcttatgta     1020

aacacaaata aaacctatga ttattataaa aacaaattaa atagaaacag tattgataat     1080

aagggtatga atattaatgg gtttgttcat gtaggtagaa attatggtaa tgctttttgg     1140

tacggtccat atgatgggat gttctttggc gatggcgacg gaatatactt ctcttccctt     1200

gcaaaatctt tagatgttgt aggccacgaa ttaagtcatg gtgtaacaaa taaagagtct     1260

aatcttaaat atgaaaatga atctggtgcc ctaaatgaat ctttctcaga tattatggga     1320

gtagctgttg agggtaaaaa ctttgtacta ggtgaagatt gctgggttgc tggaggagta     1380

atgagagata tggaaaatcc atccagagga ggccaaccag ctcatatgaa agattataaa     1440

tacaaaacta tgaatgacga taacggtggt gttcatacaa attcaggtat aataaaccat     1500

gctgcttatt tagttgcaga tggaatagaa aaaactggtg caaaaaatag taaagatatt     1560

atgggaaaaa tattctatac agctaattgc tataaatggg atgaaacaac aaattttgct     1620

aagtgcagaa atgatgtagt ccaagttact aaagaacttt atggcgaaaa tagcaactat     1680

gtaaaaattg ttgaaaaagc ttttgaccaa gttggaataa ctgctacacc tcaattacca     1740

ttataa                                                                1746


