                         SEQUENCE LISTING

<110>  UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED
 
<120>  Combining Genetic Traits for Furfural Tolerance

<130>  UF.1067XPCT

<150>  US 61/727,360
<151>  2012-11-16

<160>  57    

<170>  PatentIn version 3.5

<210>  1
<211>  85
<212>  DNA
<213>  Escherichia coli

<400>  1
ttgaaaatgc aatggttgac tcaggtaaaa tgtatggctc ccataaatta tttaatacat       60

cagttcctgg tttgtattac acatt                                             85


<210>  2
<211>  609
<212>  DNA
<213>  Escherichia coli

<400>  2
accacgtgac ttattaaaga tctttactgc ggctatactc ctgcgacgct aattgagcag       60

ctttttggta agattgatca aaaatggaga gaaacggggc cgaatggcga tgctactgtc      120

atattcagat atgcaacaag tacaaataat ttagttttct acaaaccgac gcagcttgga      180

cctacaggtg taaaattaca gtggagtcag ttagataccg cttctggtgg tggttttctt      240

tattgcaaca gaagtgatag cacaagtggt agcgcaatgc gtattgaaaa tgcaatggtt      300

gactcaggta aaatgtatgg ctcccataaa ttatttaata catcagttcc tggtttgtat      360

tacacattat taatttcaaa catgtggtca gcttacggta ccgtaactaa cgttagttca      420

cctgggatat acattggtga ctctgcagaa caatattttt cgtggtataa tccaagcgaa      480

gacgtgttat attggagttg caataatgcg aatagcaccc gtaaatactg ggctgtaggt      540

ggtatttatc agacccttac aattgaattc tatacagata caaactttga tccatatcag      600

gagagcatt                                                              609


<210>  3
<211>  1574
<212>  DNA
<213>  Escherichia coli

<400>  3
atccacgttt tgtattagca aacgtcaaac tctcatcgct gacagaactc accgcaaaag       60

accttctcgg ttaatgacca ggggcagtga tcgtctcatg gccttgccat ggtgttctct      120

atgttgctgg cggcgattat ctggaacctg ggtacctggt actttggttt acctgcatcc      180

agctctcata cgctgattgg cgcgatcgct tctaaagcag cccgcatgcg ttccatcgtc      240

gttcctgcgc cagaagcgca aaatgatcgg ccagcccacc aggaagccca gcgagtaaat      300

taagccgtca tagccggagg taaacaccag cgcggagatc tcatttttaa ccgcgtcaat      360

cagcattgaa gagtcctggc ttaagtcata gcccggcgga ttaaccacct gcatttccag      420

ttcaataccg agggtaaaag gttcagaaac atgaaaatcg ggtaatggca taggtttctc      480

ttaagttggc gttttctatt cagtatagaa gtcggagcgg ctgggcgaga tgcggaagtt      540

ctggaatgtt tctttttttg gtgatggtga ctgaagcaat ttggctactt ttgcaatgtg      600

acaagttatg gcacggctgg ctggtggcga agaattttga cgattgaggc atgcagaaaa      660

aaaacgggtt cagctttcag ttgatcctcc cagaactttg ctctgggggg atacggtccc      720

cgctgttccc cgtcgcttaa tctgcattat gccgcgtaac tatggcgcgg cgtttaagtt      780

tccttgccga tagcggcggc tggcagcgtt ggttctttgc cggtattgcg attggtatta      840

gcgtgatcaa attccgctgg cggttatctc tggcccaacg tttgcgaaag aactggcggc      900

aggtttacct acagctattt cgctggcctc gaccgatcag gaatgcccag tgttgtattc      960

agacgtccac gtgacttatt aaagatcttt actgcggcta tactcctgcg acgctaattg     1020

agcagctttt tggtaagatt gatcaaaaat ggagagaaac ggggccgaat ggcgatgcta     1080

ctgtcatatt cagatatgca acaagtacaa ataatttagt tttctacaaa ccgacgcagc     1140

ttggacctac aggtgtaaaa ttacagtgga gtcagttaga taccgcttct ggtggtggtt     1200

ttctttattg caacagaagt gatagcacaa gtggtagcgc aatgcgtatt gaaaatgcaa     1260

tggttgactc aggtaaaatg tatggctccc ataaattatt taatacatca gttcctggtt     1320

tgtattacac attattaatt tcaaacatgt ggtcagctta cggtaccgta actaacgtta     1380

gttcacctgg gatatacatt ggtgactctg cagaacaata tttttcgtgg tataatccaa     1440

gcgaagacgt gttatattgg agttgcaata atgcgaatag cacccgtaaa tactgggctg     1500

taggtggtat ttatcagacc cttacaattg aattctatac agatacaaac tttgatccat     1560

atcaggagag catt                                                       1574


<210>  4
<211>  1000
<212>  DNA
<213>  Escherichia coli

<400>  4
accgatttac tgtttgttgg ccttgtgcaa ctcaatgatg tggagtcatt gaaaatgatt       60

cagcgcagca gtgagctaac acaacgcctg aaatgatgca taaagcagcg actggattga      120

gattttcctg aattagtgag ctgatccgca gcaatatttt gtttatcctg tattttcaga      180

gggaatggag tgtaacgctc tgtattaaca aggagagcat taaaatgggt aaactcacgg      240

gcaagacagc actgattacg ggcgcattgc agggaattgg cgaaggaatt gccagaactt      300

ttgcacgtca tggcgcgaac ctaatcttgc tggatatctc ccctgagatc gaaaagctgg      360

cggacgaact gtgtggtcgt ggtcatcgct gtacggcggt tgtcgccgat gtgcgtgacc      420

cggcgtcggt agccgcagct atcaaacgcg cgaaggaaaa agaagggcgc attgatatcc      480

tggtgaataa cgcaggcgtt tgtcgtctgg gcagtttcct cgatatgagc gatgacgatc      540

gcgatttcca tattgacatc aatattaaag gcgtatggaa cgtcacgaag gcggtgctgc      600

cggagatgat tgcccgcaaa gatggtcgca ttgtgatgat gtcttcagtc actggtgata      660

tggtggccga tcctggcgaa caggcgtacg ccttaacgaa agcggcgatt gttggcctga      720

caaaatcgct ggcggtggag tacgcgcagt ctggtattcg cgttaacgcc atttgcccgg      780

gatacgtgcg cacaccaatg gcggaaagca ttgcccgcca gtcgaacccg gaagatccag      840

agtcggtgct gactgaaatg gcgaaagcaa tcccgatgcg tcgcctcgcc gatccgctgg      900

aagtcggcga actggcggcc ttcctcgcat cggatgaatc cagctattta accggtacac      960

agaatgtgat tgatggcggc agcacactgc cggagacggt                           1000


<210>  5
<211>  263
<212>  PRT
<213>  Escherichia coli

<400>  5

Met Gly Lys Leu Thr Gly Lys Thr Ala Leu Ile Thr Gly Ala Leu Gln 
1               5                   10                  15      


Gly Ile Gly Glu Gly Ile Ala Arg Thr Phe Ala Arg His Gly Ala Asn 
            20                  25                  30          


Leu Ile Leu Leu Asp Ile Ser Pro Glu Ile Glu Lys Leu Ala Asp Glu 
        35                  40                  45              


Leu Cys Gly Arg Gly His Arg Cys Thr Ala Val Val Ala Asp Val Arg 
    50                  55                  60                  


Asp Pro Ala Ser Val Ala Ala Ala Ile Lys Arg Ala Lys Glu Lys Glu 
65                  70                  75                  80  


Gly Arg Ile Asp Ile Leu Val Asn Asn Ala Gly Val Cys Arg Leu Gly 
                85                  90                  95      


Ser Phe Leu Asp Met Ser Asp Asp Asp Arg Asp Phe His Ile Asp Ile 
            100                 105                 110         


Asn Ile Lys Gly Val Trp Asn Val Thr Lys Ala Val Leu Pro Glu Met 
        115                 120                 125             


Ile Ala Arg Lys Asp Gly Arg Ile Val Met Met Ser Ser Val Thr Gly 
    130                 135                 140                 


Asp Met Val Ala Asp Pro Gly Glu Gln Ala Tyr Ala Leu Thr Lys Ala 
145                 150                 155                 160 


Ala Ile Val Gly Leu Thr Lys Ser Leu Ala Val Glu Tyr Ala Gln Ser 
                165                 170                 175     


Gly Ile Arg Val Asn Ala Ile Cys Pro Gly Tyr Val Arg Thr Pro Met 
            180                 185                 190         


Ala Glu Ser Ile Ala Arg Gln Ser Asn Pro Glu Asp Pro Glu Ser Val 
        195                 200                 205             


Leu Thr Glu Met Ala Lys Ala Ile Pro Met Arg Arg Leu Ala Asp Pro 
    210                 215                 220                 


Leu Glu Val Gly Glu Leu Ala Ala Phe Leu Ala Ser Asp Glu Ser Ser 
225                 230                 235                 240 


Tyr Leu Thr Gly Thr Gln Asn Val Ile Asp Gly Gly Ser Thr Leu Pro 
                245                 250                 255     


Glu Thr Val Ser Val Gly Ile 
            260             


<210>  6
<211>  1149
<212>  DNA
<213>  Escherichia coli

<400>  6
ttaccaggcg gtatggtaaa gctctacaat atcctcaagc gttgcttcac gcgggttgcc       60

accggtacaa acatcatcca gtgccgcctg agccagtgcc ggaatgtctt ccttgcgtac      120

accaacatca cgcaaatgtg gcggaatacc gacatcacgg ttgagagcaa acaccgcttc      180

aacagcggca ttacgcgcct cttccaggct cataccttcc actttcacgc ccataacgcg      240

cgcgatatcg cggtacttct caccggtaaa gtcagcgtta tagcgcatga catgcggtaa      300

caggatggcg ttcgcaacac cgtgtggagt gttgtaaaac gcgcccagtg gatgcgccat      360

accatgcacc aaccctaacc caacattcga gaagcccata cccgcaacat actgcccgag      420

cgccatttct tctccggcat ccttatcacc agcaaccgat cctcgcagcg ccccagcaat      480

gatttcaatc gctttaatgt gcagtgcatc ggttagcgcc cacgcgccac gggtaatata      540

cccctcaata gcatgagtga gcgcatcgac acccgtcgca gctttcagcg ctggaggcat      600

accatccatc atgtcagcgt caataaacgc cacctgcggg atatcatgcg gatcaacgca      660

aacaaacttg cgccgttttt cttcgtcagt gatcacgtag ttaatggtca cttctgccgc      720

agtgcctgct gtggtgggga ttgccagaat cggtacactg ggtttattgg tcggggaaag      780

tccttccagg ctacgcacat cggcaaactc cgggttgttg ctgataatgc caatcgcttt      840

acaagtatcc tgtggagaac caccaccaat agcgatcagg taatccgcgc cgctattctg      900

gaatacaccg agcccttctt tgacgacagt aattgttggg ttgggcacta cgccgtcgta      960

aatcgcccat gccagccctg cagcatccat cttatcggtc actttcgcca ccacgccgca     1020

ttgcaccagc gttttatcgg tgacgatcag cgccttctga taaccacggc gtttcacctc     1080

atcggttaaa gccccaacag caccccgacc aaaccatgcc gtttcgttca gaatcattct     1140

gttagccat                                                             1149


<210>  7
<211>  382
<212>  PRT
<213>  Escherichia coli

<400>  7

Met Ala Asn Arg Met Ile Leu Asn Glu Thr Ala Trp Phe Gly Arg Gly 
1               5                   10                  15      


Ala Val Gly Ala Leu Thr Asp Glu Val Lys Arg Arg Gly Tyr Gln Lys 
            20                  25                  30          


Ala Leu Ile Val Thr Asp Lys Thr Leu Val Gln Cys Gly Val Val Ala 
        35                  40                  45              


Lys Val Thr Asp Lys Met Asp Ala Ala Gly Leu Ala Trp Ala Ile Tyr 
    50                  55                  60                  


Asp Gly Val Val Pro Asn Pro Thr Ile Thr Val Val Lys Glu Gly Leu 
65                  70                  75                  80  


Gly Val Phe Gln Asn Ser Gly Ala Asp Tyr Leu Ile Ala Ile Gly Gly 
                85                  90                  95      


Gly Ser Pro Gln Asp Thr Cys Lys Ala Ile Gly Ile Ile Ser Asn Asn 
            100                 105                 110         


Pro Glu Phe Ala Asp Val Arg Ser Leu Glu Gly Leu Ser Pro Thr Asn 
        115                 120                 125             


Lys Pro Ser Val Pro Ile Leu Ala Ile Pro Thr Thr Ala Gly Thr Ala 
    130                 135                 140                 


Ala Glu Val Thr Ile Asn Tyr Val Ile Thr Asp Glu Glu Lys Arg Arg 
145                 150                 155                 160 


Lys Phe Val Cys Val Asp Pro His Asp Ile Pro Gln Val Ala Phe Ile 
                165                 170                 175     


Asp Ala Asp Met Met Asp Gly Met Pro Pro Ala Leu Lys Ala Ala Thr 
            180                 185                 190         


Gly Val Asp Ala Leu Thr His Ala Ile Glu Gly Tyr Ile Thr Arg Gly 
        195                 200                 205             


Ala Trp Ala Leu Thr Asp Ala Leu His Ile Lys Ala Ile Glu Ile Ile 
    210                 215                 220                 


Ala Gly Ala Leu Arg Gly Ser Val Ala Gly Asp Lys Asp Ala Gly Glu 
225                 230                 235                 240 


Glu Met Ala Leu Gly Gln Tyr Val Ala Gly Met Gly Phe Ser Asn Val 
                245                 250                 255     


Gly Leu Gly Leu Val His Gly Met Ala His Pro Leu Gly Ala Phe Tyr 
            260                 265                 270         


Asn Thr Pro His Gly Val Ala Asn Ala Ile Leu Leu Pro His Val Met 
        275                 280                 285             


Arg Tyr Asn Ala Asp Phe Thr Gly Glu Lys Tyr Arg Asp Ile Ala Arg 
    290                 295                 300                 


Val Met Gly Val Lys Val Glu Gly Met Ser Leu Glu Glu Ala Arg Asn 
305                 310                 315                 320 


Ala Ala Val Glu Ala Val Phe Ala Leu Asn Arg Asp Val Gly Ile Pro 
                325                 330                 335     


Pro His Leu Arg Asp Val Gly Val Arg Lys Glu Asp Ile Pro Ala Leu 
            340                 345                 350         


Ala Gln Ala Ala Leu Asp Asp Val Cys Thr Gly Gly Asn Pro Arg Glu 
        355                 360                 365             


Ala Thr Leu Glu Asp Ile Val Glu Leu Tyr His Thr Ala Trp 
    370                 375                 380         


<210>  8
<211>  1164
<212>  DNA
<213>  Artificial sequence

<220>
<223>  yqhD nucleic acid sequence

<400>  8
atgaacaact ttaatctgca caccccaacc cgcattctgt ttggtaaagg cgcaatcgct       60

ggtttacgcg aacaaattcc tcacgatgct cgcgtattga ttacctacgg cggcggcagc      120

gtgaaaaaaa ccggcgttct cgatcaagtt ctggatgccc tgaaaggcat ggacgtgctg      180

gaatttggcg gtattgagcc aaacccggct tatgaaacgc tgatgaacgc cgtgaaactg      240

gttcgcgaac agaaagtgac tttcctgctg gcggttggcg gcggttctgt actggacggc      300

accaaattta tcgccgcagc ggctaactat ccggaaaata tcgatccgtg gcacattctg      360

caaacgggcg gtaaagagat taaaagcgcc atcccgatgg gctgtgtgct gacgctgcca      420

gcaaccggtt cagaatccaa cgcaggcgcg gtgatctccc gtaaaaccac aggcgacaag      480

caggcgttcc attctgccca tgttcagccg gtatttgccg tgctcgatcc ggtttatacc      540

tacaccctgc cgccgcgtca ggtggctaac ggcgtagtgg acgcctttgt acacaccgtg      600

gaacagtatg ttaccaaacc ggttgatgcc aaaattcagg accgtttcgc agaaggcatt      660

ttgctgacgc taatcgaaga tggtccgaaa gccctgaaag agccagaaaa ctacgatgtg      720

cgcgccaacg tcatgtgggc ggcgactcag gcgctgaacg gtttgattgg cgctggcgta      780

ccgcaggact gggcaacgca tatgctgggc cacgaactga ctgcgatgca cggtctggat      840

cacgcgcaaa cactggctat cgtcctgcct gcactgtgga atgaaaaacg cgataccaag      900

cgcgctaagc tgctgcaata tgctgaacgc gtctggaaca tcactgaagg ttccgatgat      960

gagcgtattg acgccgcgat tgccgcaacc cgcaatttct ttgagcaatt aggcgtgccg     1020

acccacctct ccgactacgg tctggacggc agctccatcc cggctttgct gaaaaaactg     1080

gaagagcacg gcatgaccca actgggcgaa aatcatgaca ttacgttgga tgtcagccgc     1140

cgtatatacg aagccgcccg ctaa                                            1164


<210>  9
<211>  387
<212>  PRT
<213>  Artificial sequence

<220>
<223>  yqhD amino acid sequence

<400>  9

Met Asn Asn Phe Asn Leu His Thr Pro Thr Arg Ile Leu Phe Gly Lys 
1               5                   10                  15      


Gly Ala Ile Ala Gly Leu Arg Glu Gln Ile Pro His Asp Ala Arg Val 
            20                  25                  30          


Leu Ile Thr Tyr Gly Gly Gly Ser Val Lys Lys Thr Gly Val Leu Asp 
        35                  40                  45              


Gln Val Leu Asp Ala Leu Lys Gly Met Asp Val Leu Glu Phe Gly Gly 
    50                  55                  60                  


Ile Glu Pro Asn Pro Ala Tyr Glu Thr Leu Met Asn Ala Val Lys Leu 
65                  70                  75                  80  


Val Arg Glu Gln Lys Val Thr Phe Leu Leu Ala Val Gly Gly Gly Ser 
                85                  90                  95      


Val Leu Asp Gly Thr Lys Phe Ile Ala Ala Ala Ala Asn Tyr Pro Glu 
            100                 105                 110         


Asn Ile Asp Pro Trp His Ile Leu Gln Thr Gly Gly Lys Glu Ile Lys 
        115                 120                 125             


Ser Ala Ile Pro Met Gly Cys Val Leu Thr Leu Pro Ala Thr Gly Ser 
    130                 135                 140                 


Glu Ser Asn Ala Gly Ala Val Ile Ser Arg Lys Thr Thr Gly Asp Lys 
145                 150                 155                 160 


Gln Ala Phe His Ser Ala His Val Gln Pro Val Phe Ala Val Leu Asp 
                165                 170                 175     


Pro Val Tyr Thr Tyr Thr Leu Pro Pro Arg Gln Val Ala Asn Gly Val 
            180                 185                 190         


Val Asp Ala Phe Val His Thr Val Glu Gln Tyr Val Thr Lys Pro Val 
        195                 200                 205             


Asp Ala Lys Ile Gln Asp Arg Phe Ala Glu Gly Ile Leu Leu Thr Leu 
    210                 215                 220                 


Ile Glu Asp Gly Pro Lys Ala Leu Lys Glu Pro Glu Asn Tyr Asp Val 
225                 230                 235                 240 


Arg Ala Asn Val Met Trp Ala Ala Thr Gln Ala Leu Asn Gly Leu Ile 
                245                 250                 255     


Gly Ala Gly Val Pro Gln Asp Trp Ala Thr His Met Leu Gly His Glu 
            260                 265                 270         


Leu Thr Ala Met His Gly Leu Asp His Ala Gln Thr Leu Ala Ile Val 
        275                 280                 285             


Leu Pro Ala Leu Trp Asn Glu Lys Arg Asp Thr Lys Arg Ala Lys Leu 
    290                 295                 300                 


Leu Gln Tyr Ala Glu Arg Val Trp Asn Ile Thr Glu Gly Ser Asp Asp 
305                 310                 315                 320 


Glu Arg Ile Asp Ala Ala Ile Ala Ala Thr Arg Asn Phe Phe Glu Gln 
                325                 330                 335     


Leu Gly Val Pro Thr His Leu Ser Asp Tyr Gly Leu Asp Gly Ser Ser 
            340                 345                 350         


Ile Pro Ala Leu Leu Lys Lys Leu Glu Glu His Gly Met Thr Gln Leu 
        355                 360                 365             


Gly Glu Asn His Asp Ile Thr Leu Asp Val Ser Arg Arg Ile Tyr Glu 
    370                 375                 380                 


Ala Ala Arg 
385         


<210>  10
<211>  1533
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pntA nucleic acid

<400>  10
atgcgaattg gcataccaag agaacggtta accaatgaaa cccgtgttgc agcaacgcca       60

aaaacagtgg aacagctgct gaaactgggt tttaccgtcg cggtagagag cggcgcgggt      120

caactggcaa gttttgacga taaagcgttt gtgcaagcgg gcgctgaaat tgtagaaggg      180

aatagcgtct ggcagtcaga gatcattctg aaggtcaatg cgccgttaga tgatgaaatt      240

gcgttactga atcctgggac aacgctggtg agttttatct ggcctgcgca gaatccggaa      300

ttaatgcaaa aacttgcgga acgtaacgtg accgtgatgg cgatggactc tgtgccgcgt      360

atctcacgcg cacaatcgct ggacgcacta agctcgatgg cgaacatcgc cggttatcgc      420

gccattgttg aagcggcaca tgaatttggg cgcttcttta ccgggcaaat tactgcggcc      480

gggaaagtgc caccggcaaa agtgatggtg attggtgcgg gtgttgcagg tctggccgcc      540

attggcgcag caaacagtct cggcgcgatt gtgcgtgcat tcgacacccg cccggaagtg      600

aaagaacaag ttcaaagtat gggcgcggaa ttcctcgagc tggattttaa agaggaagct      660

ggcagcggcg atggctatgc caaagtgatg tcggacgcgt tcatcaaagc ggaaatggaa      720

ctctttgccg cccaggcaaa agaggtcgat atcattgtca ccaccgcgct tattccaggc      780

aaaccagcgc cgaagctaat tacccgtgaa atggttgact ccatgaaggc gggcagtgtg      840

attgtcgacc tggcagccca aaacggcggc aactgtgaat acaccgtgcc gggtgaaatc      900

ttcactacgg aaaatggtgt caaagtgatt ggttataccg atcttccggg ccgtctgccg      960

acgcaatcct cacagcttta cggcacaaac ctcgttaatc tgctgaaact gttgtgcaaa     1020

gagaaagacg gcaatatcac tgttgatttt gatgatgtgg tgattcgcgg cgtgaccgtg     1080

atccgtgcgg gcgaaattac ctggccggca ccgccgattc aggtatcagc tcagccgcag     1140

gcggcacaaa aagcggcacc ggaagtgaaa actgaggaaa aatgtacctg ctcaccgtgg     1200

cgtaaatacg cgttgatggc gctggcaatc attctttttg gctggatggc aagcgttgcg     1260

ccgaaagaat tccttgggca cttcaccgtt ttcgcgctgg cctgcgttgt cggttattac     1320

gtggtgtgga atgtatcgca cgcgctgcat acaccgttga tgtcggtcac caacgcgatt     1380

tcagggatta ttgttgtcgg agcactgttg cagattggcc agggcggctg ggttagcttc     1440

cttagtttta tcgcggtgct tatagccagc attaatattt tcggtggctt caccgtgact     1500

cagcgcatgc tgaaaatgtt ccgcaaaaat taa                                  1533


<210>  11
<211>  510
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pntA amino acid sequence

<400>  11

Met Arg Ile Gly Ile Pro Arg Glu Arg Leu Thr Asn Glu Thr Arg Val 
1               5                   10                  15      


Ala Ala Thr Pro Lys Thr Val Glu Gln Leu Leu Lys Leu Gly Phe Thr 
            20                  25                  30          


Val Ala Val Glu Ser Gly Ala Gly Gln Leu Ala Ser Phe Asp Asp Lys 
        35                  40                  45              


Ala Phe Val Gln Ala Gly Ala Glu Ile Val Glu Gly Asn Ser Val Trp 
    50                  55                  60                  


Gln Ser Glu Ile Ile Leu Lys Val Asn Ala Pro Leu Asp Asp Glu Ile 
65                  70                  75                  80  


Ala Leu Leu Asn Pro Gly Thr Thr Leu Val Ser Phe Ile Trp Pro Ala 
                85                  90                  95      


Gln Asn Pro Glu Leu Met Gln Lys Leu Ala Glu Arg Asn Val Thr Val 
            100                 105                 110         


Met Ala Met Asp Ser Val Pro Arg Ile Ser Arg Ala Gln Ser Leu Asp 
        115                 120                 125             


Ala Leu Ser Ser Met Ala Asn Ile Ala Gly Tyr Arg Ala Ile Val Glu 
    130                 135                 140                 


Ala Ala His Glu Phe Gly Arg Phe Phe Thr Gly Gln Ile Thr Ala Ala 
145                 150                 155                 160 


Gly Lys Val Pro Pro Ala Lys Val Met Val Ile Gly Ala Gly Val Ala 
                165                 170                 175     


Gly Leu Ala Ala Ile Gly Ala Ala Asn Ser Leu Gly Ala Ile Val Arg 
            180                 185                 190         


Ala Phe Asp Thr Arg Pro Glu Val Lys Glu Gln Val Gln Ser Met Gly 
        195                 200                 205             


Ala Glu Phe Leu Glu Leu Asp Phe Lys Glu Glu Ala Gly Ser Gly Asp 
    210                 215                 220                 


Gly Tyr Ala Lys Val Met Ser Asp Ala Phe Ile Lys Ala Glu Met Glu 
225                 230                 235                 240 


Leu Phe Ala Ala Gln Ala Lys Glu Val Asp Ile Ile Val Thr Thr Ala 
                245                 250                 255     


Leu Ile Pro Gly Lys Pro Ala Pro Lys Leu Ile Thr Arg Glu Met Val 
            260                 265                 270         


Asp Ser Met Lys Ala Gly Ser Val Ile Val Asp Leu Ala Ala Gln Asn 
        275                 280                 285             


Gly Gly Asn Cys Glu Tyr Thr Val Pro Gly Glu Ile Phe Thr Thr Glu 
    290                 295                 300                 


Asn Gly Val Lys Val Ile Gly Tyr Thr Asp Leu Pro Gly Arg Leu Pro 
305                 310                 315                 320 


Thr Gln Ser Ser Gln Leu Tyr Gly Thr Asn Leu Val Asn Leu Leu Lys 
                325                 330                 335     


Leu Leu Cys Lys Glu Lys Asp Gly Asn Ile Thr Val Asp Phe Asp Asp 
            340                 345                 350         


Val Val Ile Arg Gly Val Thr Val Ile Arg Ala Gly Glu Ile Thr Trp 
        355                 360                 365             


Pro Ala Pro Pro Ile Gln Val Ser Ala Gln Pro Gln Ala Ala Gln Lys 
    370                 375                 380                 


Ala Ala Pro Glu Val Lys Thr Glu Glu Lys Cys Thr Cys Ser Pro Trp 
385                 390                 395                 400 


Arg Lys Tyr Ala Leu Met Ala Leu Ala Ile Ile Leu Phe Gly Trp Met 
                405                 410                 415     


Ala Ser Val Ala Pro Lys Glu Phe Leu Gly His Phe Thr Val Phe Ala 
            420                 425                 430         


Leu Ala Cys Val Val Gly Tyr Tyr Val Val Trp Asn Val Ser His Ala 
        435                 440                 445             


Leu His Thr Pro Leu Met Ser Val Thr Asn Ala Ile Ser Gly Ile Ile 
    450                 455                 460                 


Val Val Gly Ala Leu Leu Gln Ile Gly Gln Gly Gly Trp Val Ser Phe 
465                 470                 475                 480 


Leu Ser Phe Ile Ala Val Leu Ile Ala Ser Ile Asn Ile Phe Gly Gly 
                485                 490                 495     


Phe Thr Val Thr Gln Arg Met Leu Lys Met Phe Arg Lys Asn 
            500                 505                 510 


<210>  12
<211>  400
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleic acid containing adhE promoter sequence

<400>  12
gacagcattt ttcacctcct aactacttaa aattgctatc attcgttatt gttatctagt       60

tgtgcaaaac atgctaatgt agccaccaaa tcatactaca atttattaac tgttagctat      120

aatggcgaaa agcgatgctg aaaggtgtca gctttgcaaa aatttgattt ggatcacgta      180

atcagtaccc agaagtgagt aatcttgctt acgccacctg gaagtgacgc attagagata      240

ataactctaa tgtttaaact cttttagtaa atcacagtga gtgtgagcgc gagtaagctt      300

ttgattttca taggttaagc aaatcatcac cgcactgact atactctcgt attcgagcag      360

atgatttact aaaaaagttt aacattatca ggagagcatt                            400


<210>  13
<211>  3732
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleic acid sequence for adhE::pntAB


<220>
<221>  misc_feature
<222>  (1)..(400)
<223>  adhE sequence upstream of adhE start codon

<220>
<221>  misc_feature
<222>  (401)..(1933)
<223>  pntA open reading frame

<220>
<221>  misc_feature
<222>  (1934)..(1943)
<223>  native intergenic region between pntA and pntB

<220>
<221>  misc_feature
<222>  (1944)..(3332)
<223>  pntB open reading frame

<220>
<221>  misc_feature
<222>  (3333)..(3732)
<223>  adhE sequence downstream of adhE stop codon

<400>  13
gacagcattt ttcacctcct aactacttaa aattgctatc attcgttatt gttatctagt       60

tgtgcaaaac atgctaatgt agccaccaaa tcatactaca atttattaac tgttagctat      120

aatggcgaaa agcgatgctg aaaggtgtca gctttgcaaa aatttgattt ggatcacgta      180

atcagtaccc agaagtgagt aatcttgctt acgccacctg gaagtgacgc attagagata      240

ataactctaa tgtttaaact cttttagtaa atcacagtga gtgtgagcgc gagtaagctt      300

ttgattttca taggttaagc aaatcatcac cgcactgact atactctcgt attcgagcag      360

atgatttact aaaaaagttt aacattatca ggagagcatt atgcgaattg gcataccaag      420

agaacggtta accaatgaaa cccgtgttgc agcaacgcca aaaacagtgg aacagctgct      480

gaaactgggt tttaccgtcg cggtagagag cggcgcgggt caactggcaa gttttgacga      540

taaagcgttt gtgcaagcgg gcgctgaaat tgtagaaggg aatagcgtct ggcagtcaga      600

gatcattctg aaggtcaatg cgccgttaga tgatgaaatt gcgttactga atcctgggac      660

aacgctggtg agttttatct ggcctgcgca gaatccggaa ttaatgcaaa aacttgcgga      720

acgtaacgtg accgtgatgg cgatggactc tgtgccgcgt atctcacgcg cacaatcgct      780

ggacgcacta agctcgatgg cgaacatcgc cggttatcgc gccattgttg aagcggcaca      840

tgaatttggg cgcttcttta ccgggcaaat tactgcggcc gggaaagtgc caccggcaaa      900

agtgatggtg attggtgcgg gtgttgcagg tctggccgcc attggcgcag caaacagtct      960

cggcgcgatt gtgcgtgcat tcgacacccg cccggaagtg aaagaacaag ttcaaagtat     1020

gggcgcggaa ttcctcgagc tggattttaa agaggaagct ggcagcggcg atggctatgc     1080

caaagtgatg tcggacgcgt tcatcaaagc ggaaatggaa ctctttgccg cccaggcaaa     1140

agaggtcgat atcattgtca ccaccgcgct tattccaggc aaaccagcgc cgaagctaat     1200

tacccgtgaa atggttgact ccatgaaggc gggcagtgtg attgtcgacc tggcagccca     1260

aaacggcggc aactgtgaat acaccgtgcc gggtgaaatc ttcactacgg aaaatggtgt     1320

caaagtgatt ggttataccg atcttccggg ccgtctgccg acgcaatcct cacagcttta     1380

cggcacaaac ctcgttaatc tgctgaaact gttgtgcaaa gagaaagacg gcaatatcac     1440

tgttgatttt gatgatgtgg tgattcgcgg cgtgaccgtg atccgtgcgg gcgaaattac     1500

ctggccggca ccgccgattc aggtatcagc tcagccgcag gcggcacaaa aagcggcacc     1560

ggaagtgaaa actgaggaaa aatgtacctg ctcaccgtgg cgtaaatacg cgttgatggc     1620

gctggcaatc attctttttg gctggatggc aagcgttgcg ccgaaagaat tccttgggca     1680

cttcaccgtt ttcgcgctgg cctgcgttgt cggttattac gtggtgtgga atgtatcgca     1740

cgcgctgcat acaccgttga tgtcggtcac caacgcgatt tcagggatta ttgttgtcgg     1800

agcactgttg cagattggcc agggcggctg ggttagcttc cttagtttta tcgcggtgct     1860

tatagccagc attaatattt tcggtggctt caccgtgact cagcgcatgc tgaaaatgtt     1920

ccgcaaaaat taaggggtaa catatgtctg gaggattagt tacagctgca tacattgttg     1980

ccgcgatcct gtttatcttc agtctggccg gtctttcgaa acatgaaacg tctcgccagg     2040

gtaacaactt cggtatcgcc gggatggcga ttgcgttaat cgcaaccatt tttggaccgg     2100

atacgggtaa tgttggctgg atcttgctgg cgatggtcat tggtggggca attggtatcc     2160

gtctggcgaa gaaagttgaa atgaccgaaa tgccagaact ggtggcgatc ctgcatagct     2220

tcgtgggtct ggcggcagtg ctggttggct ttaacagcta tctgcatcat gacgcgggaa     2280

tggcaccgat tctggtcaat attcacctga cggaagtgtt cctcggtatc ttcatcgggg     2340

cggtaacgtt cacgggttcg gtggtggcgt tcggcaaact gtgtggcaag atttcgtcta     2400

aaccattgat gctgccaaac cgtcacaaaa tgaacctggc ggctctggtc gtttccttcc     2460

tgctgctgat tgtatttgtt cgcacggaca gcgtcggcct gcaagtgctg gcattgctga     2520

taatgaccgc aattgcgctg gtattcggct ggcatttagt cgcctccatc ggtggtgcag     2580

atatgccagt ggtggtgtcg atgctgaact cgtactccgg ctgggcggct gcggctgcgg     2640

gctttatgct cagcaacgac ctgctgattg tgaccggtgc gctggtcggt tcttcggggg     2700

ctatcctttc ttacattatg tgtaaggcga tgaaccgttc ctttatcagc gttattgcgg     2760

gtggtttcgg caccgacggc tcttctactg gcgatgatca ggaagtgggt gagcaccgcg     2820

aaatcaccgc agaagagaca gcggaactgc tgaaaaactc ccattcagtg atcattactc     2880

cggggtacgg catggcagtc gcgcaggcgc aatatcctgt cgctgaaatt actgagaaat     2940

tgcgcgctcg tggtattaat gtgcgtttcg gtatccaccc ggtcgcgggg cgtttgcctg     3000

gacatatgaa cgtattgctg gctgaagcaa aagtaccgta tgacatcgtg ctggaaatgg     3060

acgagatcaa tgatgacttt gctgataccg ataccgtact ggtgattggt gctaacgata     3120

cggttaaccc ggcggcgcag gatgatccga agagtccgat tgctggtatg cctgtgctgg     3180

aagtgtggaa agcgcagaac gtgattgtct ttaaacgttc gatgaacact ggctatgctg     3240

gtgtgcaaaa cccgctgttc ttcaaggaaa acacccacat gctgtttggt gacgccaaag     3300

ccagcgtgga tgcaatcctg aaagctctgt aatcagtagc gctgtctggc aacataaacg     3360

gccccttctg ggcaatgccg atcagttaag gattagttga ccgatcctta aactgaggca     3420

ctataacggc ttccacaaca gggagccgtt ttcttatgcc acttctcaat gatctgctcg     3480

atttcagtga ccatccgctt atgcctccgc cctctgcaca actatttgca gaacaccttc     3540

ccaccgagtg gatacaacac tgcctgacgc tttctgctca tgcgaccgtt cgccgccgtc     3600

gtttaccggg ggacatggtt atctggatgg tggtgcaatg agccaattac cgatgttgtt     3660

cgccgtctga acctgagcgc ggatggcgaa gcggggatga acctgctggc ccgcagcgct     3720

gtcacccagg cg                                                         3732


<210>  14
<211>  3007
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nucleic acid sequence for PyadC'fucO-ucpA

<400>  14
tgcgctacgc tctatggctc cctgacgttt ttttagccag gaccacgtga cttattaaag       60

atctttactg cggctatact cctgcgacgc taattgagca gctttttggt aagattgatc      120

aaaaatggag agaaacgggg ccgaatggcg atgctactgt catattcaga tatgcaacaa      180

gtacaaataa tttagttttc tacaaaccga cgcagcttgg acctacaggt gtaaaattac      240

agtggagtca gttagatacc gcttctggtg gtggttttct ttattgcaac agaagtgata      300

gcacaagtgg tagcgcaatg cgtattgaaa atgcaatggt tgactcaggt aaaatgtatg      360

gctcccataa attatttaat acatcagttc ctggtttgta ttacacatta ttaatttcaa      420

acatgtggtc agcttacggt accgtaacta acgttagttc acctgggata tacattggtg      480

actctgcaga acaatatttt tcgtggtata atccaagcga agacgtgtta tattggagtt      540

gcaataatgc gaatagcacc cgtaaatact gggctgtagg tggtatttat cagaccctta      600

caattgaatt ctatacagat acaaactttg atccatatca ggagagcatt atgatggcta      660

acagaatgat tctgaacgaa acggcatggt ttggtcgggg tgctgttggg gctttaaccg      720

atgaggtgaa acgccgtggt tatcagaagg cgctgatcgt caccgataaa acgctggtgc      780

aatgcggcgt ggtggcgaaa gtgaccgata agatggatgc tgcagggctg gcatgggcga      840

tttacgacgg cgtagtgccc aacccaacaa ttactgtcgt caaagaaggg ctcggtgtat      900

tccagaatag cggcgcggat tacctgatcg ctattggtgg tggttctcca caggatactt      960

gtaaagcgat tggcattatc agcaacaacc cggagtttgc cgatgtgcgt agcctggaag     1020

gactttcccc gaccaataaa cccagtgtac cgattctggc aatccccacc acagcaggca     1080

ctgcggcaga agtgaccatt aactacgtga tcactgacga agaaaaacgg cgcaagtttg     1140

tttgcgttga tccgcatgat atcccgcagg tggcgtttat tgacgctgac atgatggatg     1200

gtatgcctcc agcgctgaaa gctgcgacgg gtgtcgatgc gctcactcat gctattgagg     1260

ggtatattac ccgtggcgcg tgggcgctaa ccgatgcact gcacattaaa gcgattgaaa     1320

tcattgctgg ggcgctgcga ggatcggttg ctggtgataa ggatgccgga gaagaaatgg     1380

cgctcgggca gtatgttgcg ggtatgggct tctcgaatgt tgggttaggg ttggtgcatg     1440

gtatggcgca tccactgggc gcgttttaca acactccaca cggtgttgcg aacgccatcc     1500

tgttaccgca tgtcatgcgc tataacgctg actttaccgg tgagaagtac cgcgatatcg     1560

cgcgcgttat gggcgtgaaa gtggaaggta tgagcctgga agaggcgcgt aatgccgctg     1620

ttgaagcggt gtttgctctc aaccgtgatg tcggtattcc gccacatttg cgtgatgttg     1680

gtgtacgcaa ggaagacatt ccggcactgg ctcaggcggc actggatgat gtttgtaccg     1740

gtggcaaccc gcgtgaagca acgcttgagg atattgtaga gctttaccat accgcctggt     1800

aaaattgaag aaggaataag gtatgggtaa actcacgggc aagacagcac tgattacggg     1860

cgcattgcag ggaattggcg aaggaattgc cagaactttt gcgcgtcatg gcgcaaacct     1920

aatcttgctg gatatctccc ctgagatcga aaagctggcg gacgaactgt gtggtcgtgg     1980

tcatcgctgt acggcggttg tcgccgatgt gcgtgacccg gcgtcggtag ccgcagctat     2040

caaacgcgcg aaggaaaaag aagggcgcat tgatatcctg gtgaataacg caggcgtttg     2100

tcgtctgggc agtttcctcg atatgagcga tgaagatcgc gatttccata ttgacatcaa     2160

tattaaaggc gtatggaacg tcacgaaggc ggtgctgcca gagatgattg cgcgcaaaga     2220

tggtcgcatt gtgatgatgt cttcagtcac tggtgatatg gtggccgatc ctggcgaaac     2280

ggcgtacgcc ttaacgaaag cggcgattgt tggcctgaca aaatcgctgg cggtggagta     2340

cgcgcagtct ggtattcgcg ttaacgccat ttgcccggga tacgtgcgca caccaatggc     2400

ggaaagcatt gcccgccagt cgaacccgga agatccagag tcggtgctga ctgaaatggc     2460

gaaagcaatc ccgatgcgtc gcctcgccga tccgctggaa gtcggcgaac tggcggcctt     2520

cctcgcatcg gatgaatcca gctatttaac cggtacacag aatgtgattg atggcggcag     2580

cacactgccg gagacggtta gcgtcggtat ctgatttcac accgccagct cagctggcgg     2640

tgctgttttg taacccgcca aatcggcggt aacgaaagag gataaaccgt gtcccgtatt     2700

attatgctga tccctaccgg aaccagcgtc ggtctgacca gcgtcagcct tggcgtgatc     2760

cgtgcaatgg aacgcaaagg cgttcgtctg agcgttttca aacctatcgc tcagccgcgt     2820

accggtggcg atgcgcccga tcagactacg actatcgtgc gtgcgaactc ttccaccacg     2880

acggccgctg aaccgctgaa aatgagctac gttgaaggtc tgctttccag caatcagaaa     2940

gatgtgctga tggaagagat cgtcgcaaac taccacgcta acaccaaaga cgctgaagtc     3000

gtttaat                                                               3007


<210>  15
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  intergenic sequence

<400>  15
aattgaagaa ggaataaggt                                                   20


<210>  16
<211>  1777
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(178)
<223>  ackA' upstream sequence

<220>
<221>  misc_feature
<222>  (1750)..(1777)
<223>  fucO downstream ORF

<400>  16
tacttgagtc gtcaaattca tatacattat gccattggct gaaaattacg caaaatggca       60

tagactcaag atatttcttc catcatgcaa aaaaaatttg cagtgcatga tgttaatcat      120

aaatgtcggt gtcatcatgc gctacgctct atggctccct gacgtttttt tagccaggat      180

ccacgttttg tattagcaaa cgtcaaactc tcatcgctgc aaatcaccgc aaaagacctt      240

ctcggttaat gaccaggggc agtgatcgtc tcatggcctt gccatggtgt tctctatgtt      300

gctggcggcg attatctgga acctgggtac ctggtacttt ggtttacctg catccagctc      360

tcatacgctg attggcgcga tcgcttctaa agcagcccgc atgcgttcca tcgtcgttcc      420

tgcgccagaa gcgcaaaatg atcggccagc ccaccaggaa gcccagcgag taaattaagc      480

cgtcatagcc ggaggtaaac accagcgcgg agatctcatt tttaaccgcg tcaatcagca      540

ttgaagagtc ctggcttaag tcatagcccg gcggattaac cacctgcatt tccagttcaa      600

taccgagggt aaaaggttca gaaacatgaa aatcgggtaa tggcataggt ttctcttaag      660

ttggcgtttt ctattcagta tagaagtcgg agcggctggg cgagatgcgg aagttctgga      720

atgtttcttt ttttggtgat ggtgactgaa gcaatttggc tacttttgca atgtgacaag      780

ttatggcacg gctggctggt ggcgaagaat tttgacgatt gaggcatgca gaaaaaaaac      840

gggttcagct ttcagttgat cctcccagaa ctttgctctg gggggatacg gtccccgctg      900

ttccccgtcg cttaatctgc attatgccgc gtaactatgg cgcggcgttt aagtttcctt      960

gccgatagcg gcggctggca gcgttggttc tttgccggta ttgcgattgg tattagcgtg     1020

atcaaattcc gctggcggtt atctctggcc caacgtttgc gaaagaactg gcggcaggtt     1080

tacctacagc tatttcgctg gcctcgaccg atcaggaatg cccagtgttg tattcagacg     1140

tccacgtgac ttattaaaga tctttactgc ggctatactc ctgcgacgct aattgagcag     1200

ctttttggta agattgatca aaaatggaga gaaacggggc cgaatggcga tgctactgtc     1260

atattcagat atgcaacaag tacaaataat ttagttttct acaaaccgac gcagcttgga     1320

cctacaggtg taaaattaca gtggagtcag ttagataccg cttctggtgg tggttttctt     1380

tattgcaaca gaagtgatag cacaagtggt agcgcaatgc gtattgaaaa tgcaatggtt     1440

gactcaggta aaatgtatgg ctcccataaa ttatttaata catcagttcc tggtttgtat     1500

tacacattat taatttcaaa catgtggtca gcttacggta ccgtaactaa cgttagttca     1560

cctgggatat acattggtga ctctgcagaa caatattttt cgtggtataa tccaagcgaa     1620

gacgtgttat attggagttg caataatgcg aatagcaccc gtaaatactg ggctgtaggt     1680

ggtatttatc agacccttac aattgaattc tatacagata caaactttga tccatatcag     1740

gagagcatta tgatggctaa cagaatgatt ctgaacg                              1777


<210>  17
<211>  808
<212>  DNA
<213>  Escherichia coli


<220>
<221>  misc_feature
<222>  (1)..(171)
<223>  ackA' upstream sequence

<220>
<221>  misc_feature
<222>  (781)..(808)
<223>  fucO ORF

<400>  17
gtcgtcaaat tcatatacat tatgccattg gctgaaaatt acgcaaaatg gcatagactc       60

aagatatttc ttccatcatg caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc      120

ggtgtcatca tgcgctacgc tctatggctc cctgacgttt ttttagccag gaccacgtga      180

cttattaaag atctttactg cggctatact cctgcgacgc taattgagca gctttttggt      240

aagattgatc aaaaatggag agaaacgggg ccgaatggcg atgctactgt catattcaga      300

tatgcaacaa gtacaaataa tttagttttc tacaaaccga cgcagcttgg acctacaggt      360

gtaaaattac agtggagtca gttagatacc gcttctggtg gtggttttct ttattgcaac      420

agaagtgata gcacaagtgg tagcgcaatg cgtattgaaa atgcaatggt tgactcaggt      480

aaaatgtatg gctcccataa attatttaat acatcagttc ctggtttgta ttacacatta      540

ttaatttcaa acatgtggtc agcttacggt accgtaacta acgttagttc acctgggata      600

tacattggtg actctgcaga acaatatttt tcgtggtata atccaagcga agacgtgtta      660

tattggagtt gcaataatgc gaatagcacc cgtaaatact gggctgtagg tggtatttat      720

cagaccctta caattgaatt ctatacagat acaaactttg atccatatca ggagagcatt      780

atgatggcta acagaatgat tctgaacg                                         808


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  yqhD up

<400>  18
tatgatgcca ggctcgtaca                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  yqhD Down

<400>  19
gatcatgcct ttccatgctt                                                   20


<210>  20
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  yqhD 1

<400>  20
gctttttacg cctcaaactt tcgt                                              24


<210>  21
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  yqhD 2

<400>  21
tacttgctcc ctttgctgg                                                    19


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adhE up

<400>  22
caatacgcct tttgacagca                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adhE down

<400>  23
gccatcaatg gcaaaaagtt                                                   20


<210>  24
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adhE-pntAB ORF up

<400>  24
tactaaaaaa gtttaacatt atcaggagag cattatgcga attggcatac caagag           56


<210>  25
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adhE-pntAB ORF down

<400>  25
tgccagacag cgctactgat tacagagctt tcaggattgc a                           41


<210>  26
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adhE-pntAB 1

<400>  26
tgcaatcctg aaagctctgt aatcagtagc gctgtctggc a                           41


<210>  27
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adhE-pntAB 2

<400>  27
ctcttggtat gccaattcgc ataatgctct cctgataatg ttaaactttt ttagta           56


<210>  28
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTrcFucO-UcpA left

<400>  28
cttgcccgtg agtttaccca taccttattc cttcttcaat tttaccaggc ggtatggtaa       60

agct                                                                    64


<210>  29
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTrcFucO-UcpA right

<400>  29
cggttagcgt cggtatctga atgcgctgat gtgataatgc cggat                       45


<210>  30
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTrcFucO-UcpA ORFup

<400>  30
aattgaagaa ggaataaggt atgggtaaac tcacgggcaa g                           41


<210>  31
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTrcFucO-UcpA ORF down

<400>  31
atccggcatt atcacatcag cgcattcaga taccgacgct aaccg                       45


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ackA 10

<400>  32
gactcttccg gcatagtctg                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ackA 20

<400>  33
gcatgagcgt tgacgcaatc                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ackA up

<400>  34
ctggttctga actgcggtag                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ackA down

<400>  35
cgcgataacc agttcttcgt                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ackAup 200

<400>  36
ttagcagcct gaaggcctaa                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ackAdown 200

<400>  37
acgacttcag cgtctttggt                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC-up

<400>  38
cacctcgcta acggattcac                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC-down

<400>  39
ggatgacgat gagcgcattg                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ackA 1

<400>  40
tttcacaccg ccagctcagc                                                   20


<210>  41
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ackA 2

<400>  41
ggaagtacct ataattgata cgtggctaaa aaaacgt                                37


<210>  42
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ackApAC up

<400>  42
gtatcaatta taggtacttc catgatggct aacagaatga ttctg                       45


<210>  43
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ackApAC down

<400>  43
gctgagctgg cggtgtgaaa tcagataccg acgctaaccg tctcc                       45


<210>  44
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC PacI

<400>  44
gcatttaatt aacctgtgga acacctacat ct                                     32


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pACYC HindIII

<400>  45
aaccaagcct atgcctacag                                                   20


<210>  46
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HindIII ackA fucO

<400>  46
gcataagctt ttagcagcct gaaggcctaa gtagtacata ttcat                       45


<210>  47
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ackA fucO PacI

<400>  47
gcatttaatt aaacgacttc agcgtctttg gtgttagcgt g                           41


<210>  48
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fucO RBS

<400>  48
tatcaggaga gcattatgat ggctaacaga atgattctga acgaaacg                    48


<210>  49
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fucO BamHI

<400>  49
ggatcctggc taaaaaaacg tcagggagcc atagagcgta gcgcatgatg a                51


<210>  50
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adhE-fucO ORF up

<400>  50
tactaaaaaa gtttaacatt atcaggagag cattatgatg gctaacagaa tgattctgaa       60

c                                                                       61


<210>  51
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adhE-fucO ORF down

<400>  51
tgccagacag cgctactgat taccaggcgg tatggtaaag                             40


<210>  52
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adhE-fucO 1

<400>  52
ctttaccata ccgcctggta atcagtagcg ctgtctggca                             40


<210>  53
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  adhE-fucO 2

<400>  53
gttcagaatc attctgttag ccatcataat gctctcctga taatgttaaa cttttttagt       60

a                                                                       61


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fucO ORF left

<400>  54
accagcgttt tatcggtgac                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ackA up 200

<400>  55
ttagcagcct gaaggcctaa                                                   20


<210>  56
<211>  1389
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pntB nucleic acid sequence

<400>  56
atgtctggag gattagttac agctgcatac attgttgccg cgatcctgtt tatcttcagt       60

ctggccggtc tttcgaaaca tgaaacgtct cgccagggta acaacttcgg tatcgccggg      120

atggcgattg cgttaatcgc aaccattttt ggaccggata cgggtaatgt tggctggatc      180

ttgctggcga tggtcattgg tggggcaatt ggtatccgtc tggcgaagaa agttgaaatg      240

accgaaatgc cagaactggt ggcgatcctg catagcttcg tgggtctggc ggcagtgctg      300

gttggcttta acagctatct gcatcatgac gcgggaatgg caccgattct ggtcaatatt      360

cacctgacgg aagtgttcct cggtatcttc atcggggcgg taacgttcac gggttcggtg      420

gtggcgttcg gcaaactgtg tggcaagatt tcgtctaaac cattgatgct gccaaaccgt      480

cacaaaatga acctggcggc tctggtcgtt tccttcctgc tgctgattgt atttgttcgc      540

acggacagcg tcggcctgca agtgctggca ttgctgataa tgaccgcaat tgcgctggta      600

ttcggctggc atttagtcgc ctccatcggt ggtgcagata tgccagtggt ggtgtcgatg      660

ctgaactcgt actccggctg ggcggctgcg gctgcgggct ttatgctcag caacgacctg      720

ctgattgtga ccggtgcgct ggtcggttct tcgggggcta tcctttctta cattatgtgt      780

aaggcgatga accgttcctt tatcagcgtt attgcgggtg gtttcggcac cgacggctct      840

tctactggcg atgatcagga agtgggtgag caccgcgaaa tcaccgcaga agagacagcg      900

gaactgctga aaaactccca ttcagtgatc attactccgg ggtacggcat ggcagtcgcg      960

caggcgcaat atcctgtcgc tgaaattact gagaaattgc gcgctcgtgg tattaatgtg     1020

cgtttcggta tccacccggt cgcggggcgt ttgcctggac atatgaacgt attgctggct     1080

gaagcaaaag taccgtatga catcgtgctg gaaatggacg agatcaatga tgactttgct     1140

gataccgata ccgtactggt gattggtgct aacgatacgg ttaacccggc ggcgcaggat     1200

gatccgaaga gtccgattgc tggtatgcct gtgctggaag tgtggaaagc gcagaacgtg     1260

attgtcttta aacgttcgat gaacactggc tatgctggtg tgcaaaaccc gctgttcttc     1320

aaggaaaaca cccacatgct gtttggtgac gccaaagcca gcgtggatgc aatcctgaaa     1380

gctctgtaa                                                             1389


<210>  57
<211>  462
<212>  PRT
<213>  Artificial sequence

<220>
<223>  pntB amino acid sequence

<400>  57

Met Ser Gly Gly Leu Val Thr Ala Ala Tyr Ile Val Ala Ala Ile Leu 
1               5                   10                  15      


Phe Ile Phe Ser Leu Ala Gly Leu Ser Lys His Glu Thr Ser Arg Gln 
            20                  25                  30          


Gly Asn Asn Phe Gly Ile Ala Gly Met Ala Ile Ala Leu Ile Ala Thr 
        35                  40                  45              


Ile Phe Gly Pro Asp Thr Gly Asn Val Gly Trp Ile Leu Leu Ala Met 
    50                  55                  60                  


Val Ile Gly Gly Ala Ile Gly Ile Arg Leu Ala Lys Lys Val Glu Met 
65                  70                  75                  80  


Thr Glu Met Pro Glu Leu Val Ala Ile Leu His Ser Phe Val Gly Leu 
                85                  90                  95      


Ala Ala Val Leu Val Gly Phe Asn Ser Tyr Leu His His Asp Ala Gly 
            100                 105                 110         


Met Ala Pro Ile Leu Val Asn Ile His Leu Thr Glu Val Phe Leu Gly 
        115                 120                 125             


Ile Phe Ile Gly Ala Val Thr Phe Thr Gly Ser Val Val Ala Phe Gly 
    130                 135                 140                 


Lys Leu Cys Gly Lys Ile Ser Ser Lys Pro Leu Met Leu Pro Asn Arg 
145                 150                 155                 160 


His Lys Met Asn Leu Ala Ala Leu Val Val Ser Phe Leu Leu Leu Ile 
                165                 170                 175     


Val Phe Val Arg Thr Asp Ser Val Gly Leu Gln Val Leu Ala Leu Leu 
            180                 185                 190         


Ile Met Thr Ala Ile Ala Leu Val Phe Gly Trp His Leu Val Ala Ser 
        195                 200                 205             


Ile Gly Gly Ala Asp Met Pro Val Val Val Ser Met Leu Asn Ser Tyr 
    210                 215                 220                 


Ser Gly Trp Ala Ala Ala Ala Ala Gly Phe Met Leu Ser Asn Asp Leu 
225                 230                 235                 240 


Leu Ile Val Thr Gly Ala Leu Val Gly Ser Ser Gly Ala Ile Leu Ser 
                245                 250                 255     


Tyr Ile Met Cys Lys Ala Met Asn Arg Ser Phe Ile Ser Val Ile Ala 
            260                 265                 270         


Gly Gly Phe Gly Thr Asp Gly Ser Ser Thr Gly Asp Asp Gln Glu Val 
        275                 280                 285             


Gly Glu His Arg Glu Ile Thr Ala Glu Glu Thr Ala Glu Leu Leu Lys 
    290                 295                 300                 


Asn Ser His Ser Val Ile Ile Thr Pro Gly Tyr Gly Met Ala Val Ala 
305                 310                 315                 320 


Gln Ala Gln Tyr Pro Val Ala Glu Ile Thr Glu Lys Leu Arg Ala Arg 
                325                 330                 335     


Gly Ile Asn Val Arg Phe Gly Ile His Pro Val Ala Gly Arg Leu Pro 
            340                 345                 350         


Gly His Met Asn Val Leu Leu Ala Glu Ala Lys Val Pro Tyr Asp Ile 
        355                 360                 365             


Val Leu Glu Met Asp Glu Ile Asn Asp Asp Phe Ala Asp Thr Asp Thr 
    370                 375                 380                 


Val Leu Val Ile Gly Ala Asn Asp Thr Val Asn Pro Ala Ala Gln Asp 
385                 390                 395                 400 


Asp Pro Lys Ser Pro Ile Ala Gly Met Pro Val Leu Glu Val Trp Lys 
                405                 410                 415     


Ala Gln Asn Val Ile Val Phe Lys Arg Ser Met Asn Thr Gly Tyr Ala 
            420                 425                 430         


Gly Val Gln Asn Pro Leu Phe Phe Lys Glu Asn Thr His Met Leu Phe 
        435                 440                 445             


Gly Asp Ala Lys Ala Ser Val Asp Ala Ile Leu Lys Ala Leu 
    450                 455                 460         


