                         SEQUENCE LISTING

<110>  Olink AB
 
<120>  RCA Reporters

<130>  27.35.114482/02

<150>  GB 1220504.3
<151>  2012-11-14

<150>  GB 1309328.1
<151>  2013-05-23

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  61
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Target


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5' Biotin

<400>  1
aaaaaaaaaa cgcgtccgcc ccgcgaaagc ctcgcctttg ccgaaaccgc gctcgtcgtc       60

g                                                                       61


<210>  2
<211>  144
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Continuous RCA two part probe part 1

<400>  2
cgacgacgag cgcggaaaag acaggcaaag cggaggggaa acaaggaaaa auuccuuguu       60

uccccuccgc uuugccuguc ugaagcggtt tttgactcga gacgaagtct cgagtcaaaa      120

accgctttgc ctgtctcgtg cttg                                             144


<210>  3
<211>  89
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Continuous RCA probe two part probe part 2


<220>
<221>  modified_base
<222>  (89)..(89)
<223>  3' Biotin

<400>  3
tgcagtgagg gctcgtttgc ggttctaaat tccttgtttc ccctcactgc acaagcacga       60

aacgcggggc ggacgcgagt gagctagac                                         89


<210>  4
<211>  158
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Cleavage strand RCA probe RCA template strand

<400>  4
cgacgacgag cgcggaaaag acaggcaaag cggaggggaa acaaggaaga gtcaaaaacc       60

gctttgcctg tctcgtgctt gtgcagtgag ggctcgtttg cggttctgaa ttccttgttt      120

cccctcactg cacaagcacg gaacgcgggg cggacgcg                              158


<210>  5
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Cleavage strand

<400>  5
uuuuugactc uuccuuguuu ccccuccgcu uugccugucu                             40


<210>  6
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Prox probe domain 1


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5' thiol

<220>
<221>  modified_base
<222>  (35)..(35)
<223>  3' RNA Ome

<400>  6
aaaaaaaaaa gacgctaata gttaagacgc ttuuu                                  35


<210>  7
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Prox probe domain 2


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5' thiol

<400>  7
aaaaaaaaaa tatgacagaa ctagacactc tt                                     32


<210>  8
<211>  182
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Two part linear RCA template strand

<400>  8
tctctctctc aagagtgtct agttctgtca tagaaagaca ggcaaagcgg aggggaaaca       60

aggaagagtc aaaaaccgct ttgcctgtct attgcttgtg cagtgagggc tcgtttgcgg      120

ttctgaattc cttgtttccc ctcactgcac aagcaatgaa aagcgtctta actattagcg      180

tc                                                                     182


<210>  9
<211>  103
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Target


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5' Biotin

<220>
<221>  modified_base
<222>  (103)..(103)
<223>  3' RNA Ome

<400>  9
tctctctctc tgctgcttcg ttgtggaagt ctcggttttc cgcgaagctt tcgttggtgg       60

cgaactcgtt tgcggttctg aattccttgt ttcccctgaa uuu                        103


<210>  10
<211>  55
<212>  DNA
<213>  Artificial sequence

<220>
<223>  RCA primer part


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5' Biotin

<400>  10
agtgagctag acaggggaaa caaggaagaa cgacgaacgt gatacgagtc aaaaa            55


<210>  11
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Connection template 1

<400>  11
ggcaaagcgg uuuuugacuc                                                   20


<210>  12
<211>  137
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Padlock 1

<400>  12
ccgctttgcc tgtctgaacg tgcttgtttc cgtgcagtgg ccagtatcac gttcgtcgtg       60

tgcgactttc tcatgttgtg cgtttggcca ctgcacggaa acaagcacgg aaagaaccgc      120

aaacgaggag tcaaaaa                                                     137


<210>  13
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Connection template 2

<400>  13
caagcggacc uuuuugacuc                                                   20


<210>  14
<211>  122
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Padlock 2

<400>  14
ggtccgcttg tcttggaagc ctttgcttcg tctgcagtgg acaaacaccg tccatacgcc       60

tgcttgtcgt gtccactgca gacgaagcaa aggcgaagcc accaacgaaa gcgagtcaaa      120

aa                                                                     122


<210>  15
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Cleavage strand 1

<400>  15
ccugcuuguc gugucccgcu uugccugucu uuuuugacuc                             40


<210>  16
<211>  58
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Ligation 1

<400>  16
agacaggcaa agcgggacac gacaagcagg gaagcggaaa accgagacga gtcaaaaa         58


<210>  17
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Cleavage strand 2

<400>  17
uguugugcgu uuggcggucc gcuugucuug uuuuugacuc                             40


<210>  18
<211>  58
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Ligation 2

<400>  18
caagacaagc ggaccgccaa acgcacaaca gaacacaacg aagcagcaga gtcaaaaa         58


<210>  19
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Cleavage strand 3

<400>  19
uuuuugacuc cgacgaacgu gauac                                             25


<210>  20
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Detection oligo 1


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5' Cy3

<400>  20
tgcgtttggc ggtccgcttg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Detection oligo 2


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5' Cy5

<400>  21
ttgtcgtgtc ccgctttgcc                                                   20


<210>  22
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Correct target


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5' Biotin

<220>
<221>  modified_base
<222>  (48)..(48)
<223>  3' RNA Ome

<400>  22
tctctctctc tctctccgcg ctcgtcgtcg cgcgtccgcc ccgcguuu                    48


<210>  23
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Wrong target


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5' Biotin

<220>
<221>  modified_base
<222>  (48)..(48)
<223>  3' RNA Ome

<400>  23
tctctctctc tctctccgcg ctcgtcgtcg agcgtccgcc ccgcguuu                    48


<210>  24
<211>  158
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Two part RCA template strand


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5' phosphorylation

<400>  24
cgacgacgag cgcggaaaag acaggcaaag cggaggggaa acaaggaaga gtcaaaaacc       60

gctttgcctg tctcgtgctt gtgcagtgag ggctcgtttg cggttctgaa ttccttgttt      120

cccctcactg cacaagcacg gaacgcgggg cggacgcg                              158


<210>  25
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Detection oligonucleotide


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5' Cy3

<400>  25
ttccttgttt cccctccgct ttgcctgtct                                        30


